AlfalfaGEDB | Msa0936690

M. sativa cultivar zhongmu-4 / Msa0936690

KEGG: K21374
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936690	XP_013450928.1	93.258	89	6	0	1	89	396	484	2.19e-52	180

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936690	sp\|Q6VAB3\|U85A8_STERE	55.682	88	39	0	1	88	392	479	1.77e-29	112

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0004340	Msa0936690	0.850171	2.017399e-60	-8.615850e-47
Msa0006050	Msa0936690	0.868372	6.856941e-66	-8.615850e-47
Msa0012820	Msa0936690	-0.807621	4.623289e-50	-8.615850e-47
Msa0014410	Msa0936690	0.809943	1.473611e-50	-8.615850e-47
Msa0040550	Msa0936690	-0.805620	1.222377e-49	-8.615850e-47
Msa0044180	Msa0936690	0.829857	4.070306e-55	-8.615850e-47
Msa0056130	Msa0936690	0.802264	6.093503e-49	-8.615850e-47
Msa0064550	Msa0936690	0.831503	1.608720e-55	-8.615850e-47
Msa0068410	Msa0936690	0.811119	8.210638e-51	-8.615850e-47
Msa0072830	Msa0936690	0.833828	4.261221e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936690	MtrunA17_Chr6g0452771	93.258	89	6	1	89	396	484	2.01e-56	180
Msa0936690	MtrunA17_Chr6g0454331	85.227	88	13	1	88	395	482	1.13e-49	162
Msa0936690	MtrunA17_Chr6g0454171	82.022	89	16	1	89	226	314	3.67e-49	157
Msa0936690	MtrunA17_Chr6g0454141	82.955	88	15	1	88	396	483	1.60e-48	159
Msa0936690	MtrunA17_Chr8g0336331	84.091	88	14	1	88	393	480	2.26e-47	156
Msa0936690	MtrunA17_Chr6g0454201	84.091	88	14	1	88	397	484	2.98e-47	156
Msa0936690	MtrunA17_Chr6g0454151	82.022	89	16	1	89	394	482	1.09e-46	155
Msa0936690	MtrunA17_Chr6g0454291	80.460	87	17	1	87	393	479	1.04e-45	152
Msa0936690	MtrunA17_Chr5g0427561	79.545	88	18	1	88	1	88	1.15e-44	139
Msa0936690	MtrunA17_Chr7g0217881	77.528	89	20	1	89	378	466	1.92e-44	148

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936690	AT1G22400.1	50.562	89	43	1	1	88	397	485	5.37e-24	94.4
Msa0936690	AT1G78270.1	50.000	86	42	1	1	85	396	481	6.11e-24	94.0
Msa0936690	AT1G22380.1	48.276	87	44	1	1	86	396	482	1.35e-23	93.2
Msa0936690	AT1G22340.1	48.864	88	44	1	1	87	397	484	4.24e-23	91.7
Msa0936690	AT1G22360.1	46.067	89	47	1	1	88	393	481	3.10e-20	83.6
Msa0936690	AT1G22370.2	52.809	89	41	1	1	88	391	479	2.49e-17	75.5
Msa0936690	AT5G59590.1	36.905	84	53	0	1	84	366	449	2.92e-17	75.1
Msa0936690	AT1G22370.1	52.809	89	41	1	1	88	221	309	2.42e-16	72.0
Msa0936690	AT3G11340.1	35.294	85	55	0	1	85	361	445	3.48e-16	72.0
Msa0936690	AT3G46660.1	35.366	82	53	0	1	82	372	453	1.66e-14	67.4

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Find 23 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTGATTAATGAATTGATGG+TGG	0.584937	6_3:-7534014	Msa0936690:CDS
AGGAAAGAAGATGAGGCAAA+AGG	0.504752	6_3:-7533984	Msa0936690:CDS
ATTTGCCAAGAATGGAAGAT+TGG	0.375696	6_3:-7534079	Msa0936690:CDS
CCAAGAATGGAAGATTGGGA+TGG	0.581034	6_3:-7534074	Msa0936690:CDS
CCATCCCAATCTTCCATTCT+TGG	0.164386	6_3:+7534074	None:intergenic
CCGGAGTGCCAATGTTGTGT+AGG	0.599133	6_3:-7534138	None:intergenic
CTGATTAATGAATTGATGGT+GGG	0.598725	6_3:-7534013	Msa0936690:CDS
CTTGGACAAAGTGATCAATG+AGG	0.725106	6_3:-7533900	Msa0936690:CDS
GAAACTGATTAATGAATTGA+TGG	0.464761	6_3:-7534017	Msa0936690:CDS
GAAGATGAGGCAAAAGGCCA+TGG	0.548548	6_3:-7533978	Msa0936690:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGGCAAATAATTCTACTGTT+AGG	+	chr6_3:7533916-7533935	None:intergenic	30.0%
GTAGAATTATTTGCCAAGAA+TGG	-	chr6_3:7533920-7533939	Msa0936690:CDS	30.0%
ACTGATTAATGAATTGATGG+TGG	-	chr6_3:7533993-7534012	Msa0936690:CDS	30.0%
TGGTTGTTCATATATGAACT+TGG	-	chr6_3:7534089-7534108	Msa0936690:CDS	30.0%
GGTAGTTGATCAGCAAAAAA+TGG	+	chr6_3:7533895-7533914	None:intergenic	35.0%
ATTTGCCAAGAATGGAAGAT+TGG	-	chr6_3:7533928-7533947	Msa0936690:CDS	35.0%
TTTGCCAAGAATGGAAGATT+GGG	-	chr6_3:7533929-7533948	Msa0936690:CDS	35.0%
TACCAATGTAAAGAGAGAAG+AGG	-	chr6_3:7533963-7533982	Msa0936690:CDS	35.0%
TACCTCTTCTCTCTTTACAT+TGG	+	chr6_3:7533968-7533987	None:intergenic	35.0%
GAGATAAAGGAAAGAAGATG+AGG	-	chr6_3:7534016-7534035	Msa0936690:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	7533880	7534149	7533880	ID=Msa0936690;Name=Msa0936690
chr6_3	mRNA	7533880	7534149	7533880	ID=Msa0936690-mRNA-1;Parent=Msa0936690;Name=Msa0936690-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|89
chr6_3	exon	7533880	7534149	7533880	ID=Msa0936690-mRNA-1:exon:2515;Parent=Msa0936690-mRNA-1
chr6_3	CDS	7533880	7534149	7533880	ID=Msa0936690-mRNA-1:cds;Parent=Msa0936690-mRNA-1

Gene Sequence	>Msa0936690 ATGTTGTGTAGGCCATTTTTTGCTGATCAACTACCTAACAGTAGAATTATTTGCCAAGAATGGAAGATTGGGATGGAAATTGATACCAATGTAAAGAGAGAAGAGGTAGAGAAACTGATTAATGAATTGATGGTGGGAGATAAAGGAAAGAAGATGAGGCAAAAGGCCATGGAGTTGAAGAAGAAGGCAGATGAGGACACAAGATTTGGTGGTTGTTCATATATGAACTTGGACAAAGTGATCAATGAGGTCTTGCTTAAACAATATTAG
Protein sequence	>Msa0936690 MLCRPFFADQLPNSRIICQEWKIGMEIDTNVKREEVEKLINELMVGDKGKKMRQKAMELKKKADEDTRFGGCSYMNLDKVINEVLLKQY*