AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1137830


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 16 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCTTCAACAACGACTCTT+TGG 0.323142 7_4:-6818130 Msa1137830:intron
TACAAAGACAGGGACCAATT+TGG 0.363523 7_4:-6818853 Msa1137830:CDS
TTGGGAGTAAACCACCAAAT+TGG 0.374281 7_4:+6818839 None:intergenic
TCTTGCAGTTGTGCTTGAGC+AGG 0.458357 7_4:-6817815 Msa1137830:CDS
TGAGAATGGCTTACAAAGAC+AGG 0.462803 7_4:-6818864 None:intergenic
CATAGGACAACAACCAAATC+AGG 0.467136 7_4:-6818176 Msa1137830:intron
CAAATCAGGCAAAAGATCAT+TGG 0.472983 7_4:-6818162 Msa1137830:CDS
AAAGACAGGGACCAATTTGG+TGG 0.516882 7_4:-6818850 Msa1137830:intron
TTGATTGTGTTCTTGAGCAA+AGG 0.535779 7_4:-6817785 Msa1137830:CDS
TAGCTTGTGTGCTTGAGCAA+AGG 0.600399 7_4:-6817755 Msa1137830:CDS
ATCATCTGAACAAACGAGGG+AGG 0.617476 7_4:-6818528 Msa1137830:CDS
TTGCAGGTGATCAAGAGAGT+CGG 0.619347 7_4:-6818573 Msa1137830:intron
TCATCTGAACAAACGAGGGA+GGG 0.623604 7_4:-6818527 Msa1137830:intron
GAGAATGGCTTACAAAGACA+GGG 0.659869 7_4:-6818863 None:intergenic
CAGATCATCTGAACAAACGA+GGG 0.691520 7_4:-6818531 Msa1137830:CDS
ACAGATCATCTGAACAAACG+AGG 0.713669 7_4:-6818532 Msa1137830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTAAAATTTAGATTTTTTT+GGG + chr7_4:6817763-6817782 None:intergenic 10.0%
!!! TGTTAATTTTAATATTTGTA+TGG - chr7_4:6817808-6817827 Msa1137830:CDS 10.0%
!!! CTTCAATTTTTTTTTTTAAT+TGG - chr7_4:6817959-6817978 Msa1137830:intron 10.0%
!!! GTTAAAATTTAGATTTTTTT+GGG + chr7_4:6817763-6817782 None:intergenic 10.0%
!!! TGTTAATTTTAATATTTGTA+TGG - chr7_4:6817808-6817827 Msa1137830:CDS 10.0%
!!! CTTCAATTTTTTTTTTTAAT+TGG - chr7_4:6817959-6817978 Msa1137830:intron 10.0%
!!! GGTTAAAATTTAGATTTTTT+TGG + chr7_4:6817764-6817783 None:intergenic 15.0%
!!! AATCTAAATTTTAACCTTTA+AGG - chr7_4:6817768-6817787 Msa1137830:CDS 15.0%
!!! ATCTAAATTTTAACCTTTAA+GGG - chr7_4:6817769-6817788 Msa1137830:CDS 15.0%
!!! GGTTAAAATTTAGATTTTTT+TGG + chr7_4:6817764-6817783 None:intergenic 15.0%
!!! AATCTAAATTTTAACCTTTA+AGG - chr7_4:6817768-6817787 Msa1137830:CDS 15.0%
!!! ATCTAAATTTTAACCTTTAA+GGG - chr7_4:6817769-6817788 Msa1137830:CDS 15.0%
!!! ATTTCAAATTTGTTCTTCAT+AGG - chr7_4:6818388-6818407 Msa1137830:intron 20.0%
!! ATTACACAGAAAATTGTTTA+AGG + chr7_4:6818727-6818746 None:intergenic 20.0%
!!! ATTTCAAATTTGTTCTTCAT+AGG - chr7_4:6818388-6818407 Msa1137830:intron 20.0%
!! ATTACACAGAAAATTGTTTA+AGG + chr7_4:6818727-6818746 None:intergenic 20.0%
! AACATGATCAATCAAAACAA+CGG + chr7_4:6817920-6817939 None:intergenic 25.0%
!!! CTGTGTAATTTTGTTTAGTT+AGG - chr7_4:6818738-6818757 Msa1137830:intron 25.0%
! AACATGATCAATCAAAACAA+CGG + chr7_4:6817920-6817939 None:intergenic 25.0%
!!! CTGTGTAATTTTGTTTAGTT+AGG - chr7_4:6818738-6818757 Msa1137830:intron 25.0%
! CAAAACAACGGTTTTTCTTA+AGG + chr7_4:6817908-6817927 None:intergenic 30.0%
TCTTTCACACCTCTATATAA+AGG - chr7_4:6818497-6818516 Msa1137830:intron 30.0%
AACACTTGATATACAATCAG+AGG + chr7_4:6818701-6818720 None:intergenic 30.0%
! CAAAACAACGGTTTTTCTTA+AGG + chr7_4:6817908-6817927 None:intergenic 30.0%
TCTTTCACACCTCTATATAA+AGG - chr7_4:6818497-6818516 Msa1137830:intron 30.0%
AACACTTGATATACAATCAG+AGG + chr7_4:6818701-6818720 None:intergenic 30.0%
!! TGGTGATGTTTATGCGATTT+TGG - chr7_4:6817979-6817998 Msa1137830:intron 35.0%
!!! GCGATTTTGGTTTATTTTGC+AGG - chr7_4:6817992-6818011 Msa1137830:intron 35.0%
TGACAACACTTTCGAAAACA+AGG + chr7_4:6818129-6818148 None:intergenic 35.0%
!!! GTTTTCGAAAGTGTTGTCAT+TGG - chr7_4:6818130-6818149 Msa1137830:intron 35.0%
CATAGATGTTCCTTTGGATA+AGG - chr7_4:6818360-6818379 Msa1137830:intron 35.0%
ATGTTCCTTTGGATAAGGAA+TGG - chr7_4:6818365-6818384 Msa1137830:intron 35.0%
GAAATCCATTCCTTATCCAA+AGG + chr7_4:6818373-6818392 None:intergenic 35.0%
! CAATGATCTTTTGCCTGATT+TGG + chr7_4:6818421-6818440 None:intergenic 35.0%
CAAATCAGGCAAAAGATCAT+TGG - chr7_4:6818419-6818438 Msa1137830:intron 35.0%
GTCTTCACTCCTTTATATAG+AGG + chr7_4:6818509-6818528 None:intergenic 35.0%
TCTTGCATTACCAATTCTCT+AGG + chr7_4:6818531-6818550 None:intergenic 35.0%
AAACAATTTCAGCAGAGGTT+TGG + chr7_4:6818590-6818609 None:intergenic 35.0%
ATGCAAAACAATTTCAGCAG+AGG + chr7_4:6818595-6818614 None:intergenic 35.0%
TTGATTGTGTTCTTGAGCAA+AGG - chr7_4:6818796-6818815 Msa1137830:intron 35.0%
!! TGGTGATGTTTATGCGATTT+TGG - chr7_4:6817979-6817998 Msa1137830:intron 35.0%
!!! GCGATTTTGGTTTATTTTGC+AGG - chr7_4:6817992-6818011 Msa1137830:intron 35.0%
TGACAACACTTTCGAAAACA+AGG + chr7_4:6818129-6818148 None:intergenic 35.0%
!!! GTTTTCGAAAGTGTTGTCAT+TGG - chr7_4:6818130-6818149 Msa1137830:intron 35.0%
CATAGATGTTCCTTTGGATA+AGG - chr7_4:6818360-6818379 Msa1137830:intron 35.0%
ATGTTCCTTTGGATAAGGAA+TGG - chr7_4:6818365-6818384 Msa1137830:intron 35.0%
GAAATCCATTCCTTATCCAA+AGG + chr7_4:6818373-6818392 None:intergenic 35.0%
! CAATGATCTTTTGCCTGATT+TGG + chr7_4:6818421-6818440 None:intergenic 35.0%
CAAATCAGGCAAAAGATCAT+TGG - chr7_4:6818419-6818438 Msa1137830:intron 35.0%
GTCTTCACTCCTTTATATAG+AGG + chr7_4:6818509-6818528 None:intergenic 35.0%
TCTTGCATTACCAATTCTCT+AGG + chr7_4:6818531-6818550 None:intergenic 35.0%
AAACAATTTCAGCAGAGGTT+TGG + chr7_4:6818590-6818609 None:intergenic 35.0%
ATGCAAAACAATTTCAGCAG+AGG + chr7_4:6818595-6818614 None:intergenic 35.0%
TTGATTGTGTTCTTGAGCAA+AGG - chr7_4:6818796-6818815 Msa1137830:intron 35.0%
TACAAAGACAGGGACCAATT+TGG - chr7_4:6817728-6817747 Msa1137830:CDS 40.0%
TTGGGAGTAAACCACCAAAT+TGG + chr7_4:6817745-6817764 None:intergenic 40.0%
! GCACTGTTCTTTTCCCTTAA+AGG + chr7_4:6817785-6817804 None:intergenic 40.0%
ACAGATCATCTGAACAAACG+AGG - chr7_4:6818049-6818068 Msa1137830:intron 40.0%
CAGATCATCTGAACAAACGA+GGG - chr7_4:6818050-6818069 Msa1137830:intron 40.0%
ACTTTCGAAAACAAGGAGAG+AGG + chr7_4:6818122-6818141 None:intergenic 40.0%
! TGTTTTCTTGTCTTGTAGCG+TGG + chr7_4:6818317-6818336 None:intergenic 40.0%
ATCCAAAGGAACATCTATGC+AGG + chr7_4:6818359-6818378 None:intergenic 40.0%
CATAGGACAACAACCAAATC+AGG - chr7_4:6818405-6818424 Msa1137830:intron 40.0%
AAGCTTCAACAACGACTCTT+TGG - chr7_4:6818451-6818470 Msa1137830:intron 40.0%
TACAAAGACAGGGACCAATT+TGG - chr7_4:6817728-6817747 Msa1137830:CDS 40.0%
TTGGGAGTAAACCACCAAAT+TGG + chr7_4:6817745-6817764 None:intergenic 40.0%
! GCACTGTTCTTTTCCCTTAA+AGG + chr7_4:6817785-6817804 None:intergenic 40.0%
ACAGATCATCTGAACAAACG+AGG - chr7_4:6818049-6818068 Msa1137830:intron 40.0%
CAGATCATCTGAACAAACGA+GGG - chr7_4:6818050-6818069 Msa1137830:intron 40.0%
ACTTTCGAAAACAAGGAGAG+AGG + chr7_4:6818122-6818141 None:intergenic 40.0%
! TGTTTTCTTGTCTTGTAGCG+TGG + chr7_4:6818317-6818336 None:intergenic 40.0%
ATCCAAAGGAACATCTATGC+AGG + chr7_4:6818359-6818378 None:intergenic 40.0%
CATAGGACAACAACCAAATC+AGG - chr7_4:6818405-6818424 Msa1137830:intron 40.0%
AAGCTTCAACAACGACTCTT+TGG - chr7_4:6818451-6818470 Msa1137830:intron 40.0%
AAAGACAGGGACCAATTTGG+TGG - chr7_4:6817731-6817750 Msa1137830:CDS 45.0%
TTGCAGGTGATCAAGAGAGT+CGG - chr7_4:6818008-6818027 Msa1137830:intron 45.0%
ATCATCTGAACAAACGAGGG+AGG - chr7_4:6818053-6818072 Msa1137830:intron 45.0%
TCATCTGAACAAACGAGGGA+GGG - chr7_4:6818054-6818073 Msa1137830:intron 45.0%
!! TTGTCATTGGTTCGTGCTGT+TGG - chr7_4:6818143-6818162 Msa1137830:CDS 45.0%
!! TGTCATTGGTTCGTGCTGTT+GGG - chr7_4:6818144-6818163 Msa1137830:CDS 45.0%
GGAGTGAAGACCTAGAGAAT+TGG - chr7_4:6818518-6818537 Msa1137830:intron 45.0%
! TAGCTTGTGTGCTTGAGCAA+AGG - chr7_4:6818826-6818845 Msa1137830:intron 45.0%
AAAGACAGGGACCAATTTGG+TGG - chr7_4:6817731-6817750 Msa1137830:CDS 45.0%
TTGCAGGTGATCAAGAGAGT+CGG - chr7_4:6818008-6818027 Msa1137830:intron 45.0%
ATCATCTGAACAAACGAGGG+AGG - chr7_4:6818053-6818072 Msa1137830:intron 45.0%
TCATCTGAACAAACGAGGGA+GGG - chr7_4:6818054-6818073 Msa1137830:intron 45.0%
!! TTGTCATTGGTTCGTGCTGT+TGG - chr7_4:6818143-6818162 Msa1137830:CDS 45.0%
!! TGTCATTGGTTCGTGCTGTT+GGG - chr7_4:6818144-6818163 Msa1137830:CDS 45.0%
GGAGTGAAGACCTAGAGAAT+TGG - chr7_4:6818518-6818537 Msa1137830:intron 45.0%
! TAGCTTGTGTGCTTGAGCAA+AGG - chr7_4:6818826-6818845 Msa1137830:intron 45.0%
!! GTCATTGGTTCGTGCTGTTG+GGG - chr7_4:6818145-6818164 Msa1137830:CDS 50.0%
!! TCATTGGTTCGTGCTGTTGG+GGG - chr7_4:6818146-6818165 Msa1137830:CDS 50.0%
ATGCAGGCGAAGCTGTATTC+TGG + chr7_4:6818343-6818362 None:intergenic 50.0%
CGCCTGCATAGATGTTCCTT+TGG - chr7_4:6818354-6818373 Msa1137830:intron 50.0%
! ACAACGACTCTTTGGTGTGC+TGG - chr7_4:6818459-6818478 Msa1137830:intron 50.0%
TCTTGCAGTTGTGCTTGAGC+AGG - chr7_4:6818766-6818785 Msa1137830:intron 50.0%
!! GTCATTGGTTCGTGCTGTTG+GGG - chr7_4:6818145-6818164 Msa1137830:CDS 50.0%
!! TCATTGGTTCGTGCTGTTGG+GGG - chr7_4:6818146-6818165 Msa1137830:CDS 50.0%
ATGCAGGCGAAGCTGTATTC+TGG + chr7_4:6818343-6818362 None:intergenic 50.0%
CGCCTGCATAGATGTTCCTT+TGG - chr7_4:6818354-6818373 Msa1137830:intron 50.0%
! ACAACGACTCTTTGGTGTGC+TGG - chr7_4:6818459-6818478 Msa1137830:intron 50.0%
TCTTGCAGTTGTGCTTGAGC+AGG - chr7_4:6818766-6818785 Msa1137830:intron 50.0%
!! CGAGGGAGGGTACTAGTTTG+CGG - chr7_4:6818067-6818086 Msa1137830:intron 55.0%
!! CGAGGGAGGGTACTAGTTTG+CGG - chr7_4:6818067-6818086 Msa1137830:intron 55.0%
!! GGGAGGGTACTAGTTTGCGG+AGG - chr7_4:6818070-6818089 Msa1137830:intron 60.0%
!! GGGAGGGTACTAGTTTGCGG+AGG - chr7_4:6818070-6818089 Msa1137830:intron 60.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 6817722 6818881 6817722 ID=Msa1137830;Name=Msa1137830
chr7_4 mRNA 6817722 6818881 6817722 ID=Msa1137830-mRNA-1;Parent=Msa1137830;Name=Msa1137830-mRNA-1;_AED=0.80;_eAED=0.80;_QI=0|0|0|0.25|1|1|4|0|93
chr7_4 exon 6818851 6818881 6818851 ID=Msa1137830-mRNA-1:exon:1927;Parent=Msa1137830-mRNA-1
chr7_4 exon 6818528 6818589 6818528 ID=Msa1137830-mRNA-1:exon:1926;Parent=Msa1137830-mRNA-1
chr7_4 exon 6818131 6818193 6818131 ID=Msa1137830-mRNA-1:exon:1925;Parent=Msa1137830-mRNA-1
chr7_4 exon 6817722 6817847 6817722 ID=Msa1137830-mRNA-1:exon:1924;Parent=Msa1137830-mRNA-1
chr7_4 CDS 6818851 6818881 6818851 ID=Msa1137830-mRNA-1:cds;Parent=Msa1137830-mRNA-1
chr7_4 CDS 6818528 6818589 6818528 ID=Msa1137830-mRNA-1:cds;Parent=Msa1137830-mRNA-1
chr7_4 CDS 6818131 6818193 6818131 ID=Msa1137830-mRNA-1:cds;Parent=Msa1137830-mRNA-1
chr7_4 CDS 6817722 6817847 6817722 ID=Msa1137830-mRNA-1:cds;Parent=Msa1137830-mRNA-1
Gene Sequence

>Msa1137830

ATGGCTTACAAAGACAGGGACCAATTTGGTGGTGATCAAGAGAGTCGGAATCTAGTTATTCATATTACAGATCATCTGAACAAACGAGGGAGGGACAACAACCAAATCAGGCAAAAGATCATTGGTGAAAGATCAAGCTTCAACAACGACTCTTTGTTAGGAAGTCTCTTGCAGTTGTGCTTGAGCAGGAAGTCTCTTGATTGTGTTCTTGAGCAAAGGAAGTCTCTAGCTTGTGTGCTTGAGCAAAGGAAGTCTCTTGCTTGTGTGCTTGAGCATTTGTAA

Protein sequence

>Msa1137830

MAYKDRDQFGGDQESRNLVIHITDHLNKRGRDNNQIRQKIIGERSSFNNDSLLGSLLQLCLSRKSLDCVLEQRKSLACVLEQRKSLACVLEHL*