AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1434040


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1434040 MtrunA17_Chr4g0068451 95.489 133 6 0 1 133 1 133 3.36e-90 262
Msa1434040 MtrunA17_Chr3g0122861 34.328 134 77 3 6 128 8 141 4.62e-19 80.1
Msa1434040 MtrunA17_Chr8g0365881 36.508 126 67 3 15 131 77 198 1.13e-14 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1434040 AT4G16210.1 80.916 131 25 0 3 133 6 136 6.39e-75 224
Msa1434040 AT5G43280.2 30.822 146 82 3 1 128 3 147 1.05e-17 76.3
Msa1434040 AT5G43280.1 30.822 146 82 3 1 128 3 147 2.49e-17 75.9
Msa1434040 AT1G60550.1 38.095 126 65 3 15 131 86 207 2.76e-15 70.9
Msa1434040 AT5G65940.2 37.805 82 49 2 1 81 5 85 2.07e-11 60.1
Msa1434040 AT5G65940.3 37.805 82 49 2 1 81 5 85 2.50e-11 60.1
Msa1434040 AT5G65940.1 37.805 82 49 2 1 81 5 85 2.76e-11 60.1
Msa1434040 AT5G65940.4 37.805 82 49 2 1 81 5 85 2.76e-11 60.1

Find 43 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGATAATTGGAGCGATTAA+AGG 0.217612 tig0027457:+59331 Msa1434040:CDS
TGCGCTTGCTTGTGATATTT+TGG 0.236137 tig0027457:+59381 Msa1434040:CDS
AGCATGAGTGTCCATGAATT+TGG 0.273255 tig0027457:-59419 None:intergenic
CCTTTAATCGCTCCAATTAT+CGG 0.278625 tig0027457:-59331 None:intergenic
CACCGCTCCATTTGCACAAC+CGG 0.282409 tig0027457:-59301 None:intergenic
CGGTGTCGTAAACCGATAAT+TGG 0.358730 tig0027457:+59319 Msa1434040:CDS
GTCACCACCGCGATTCCGTT+TGG 0.392759 tig0027457:-59079 None:intergenic
CCGCGATTCCGTTTGGTTCG+CGG 0.414612 tig0027457:-59072 None:intergenic
AGACCGGGACGAATCGGTTC+GGG 0.422302 tig0027457:+59177 Msa1434040:CDS
CTGTTCCGGCGTTGACCTTA+CGG 0.432274 tig0027457:+59231 Msa1434040:CDS
GAATCTAGACCGGGACGAAT+CGG 0.433081 tig0027457:+59171 Msa1434040:CDS
ACGGGTAGATCGTTCTGTTC+CGG 0.437262 tig0027457:+59217 Msa1434040:CDS
GAGTGACCCGGTTGTGCAAA+TGG 0.438312 tig0027457:+59294 Msa1434040:CDS
AAAGGATTCGCTGTTACAGC+TGG 0.459087 tig0027457:+59349 Msa1434040:CDS
AGTTAGCGAGTTAAGAGAAC+CGG 0.463305 tig0027457:-59113 None:intergenic
ACAGAACGATCTACCCGTTC+CGG 0.466284 tig0027457:-59212 None:intergenic
TAGACCGGGACGAATCGGTT+CGG 0.471210 tig0027457:+59176 Msa1434040:CDS
TGCTAAAGGAGCCAAATTCA+TGG 0.473611 tig0027457:+59408 Msa1434040:CDS
CAAGGGTGATGTGAAGGATC+CGG 0.474232 tig0027457:+59270 Msa1434040:CDS
CTCGTCAACCGCGAACCAAA+CGG 0.483517 tig0027457:+59064 Msa1434040:CDS
GTTCGGGTGATTATATTAAC+CGG 0.485972 tig0027457:+59193 Msa1434040:CDS
TGCACAACCGGGTCACTCTC+CGG 0.487515 tig0027457:-59289 None:intergenic
GTGATTATATTAACCGGAAC+GGG 0.488459 tig0027457:+59199 Msa1434040:CDS
TCGTGCAATGAACGTGGACC+TGG 0.500472 tig0027457:+59135 Msa1434040:CDS
GATTCTTGAAAGCTTGAGCC+AGG 0.526158 tig0027457:-59153 None:intergenic
AAGCTTTCAAGAATCTAGAC+CGG 0.546865 tig0027457:+59161 Msa1434040:CDS
AGCTTTCAAGAATCTAGACC+GGG 0.573728 tig0027457:+59162 Msa1434040:CDS
AAACATCCTCCGCTGCCGTA+AGG 0.580087 tig0027457:-59246 None:intergenic
AATTCATGGACACTCATGCT+AGG 0.584729 tig0027457:+59422 Msa1434040:CDS
ACCGCTCCATTTGCACAACC+GGG 0.599495 tig0027457:-59300 None:intergenic
TCACCCGAACCGATTCGTCC+CGG 0.601302 tig0027457:-59180 None:intergenic
GTGGTGACTATTAACCGTCC+CGG 0.603318 tig0027457:+59094 Msa1434040:CDS
CGTTGACCTTACGGCAGCGG+AGG 0.606251 tig0027457:+59240 Msa1434040:CDS
GGTGATTATATTAACCGGAA+CGG 0.610789 tig0027457:+59198 Msa1434040:CDS
CGCTGCCGTAAGGTCAACGC+CGG 0.612184 tig0027457:-59236 None:intergenic
AATCGGGTCGAACATGACAT+CGG 0.623182 tig0027457:+59030 None:intergenic
GAAGGATCCGGAGAGTGACC+CGG 0.627080 tig0027457:+59282 Msa1434040:CDS
CCGCGAACCAAACGGAATCG+CGG 0.633717 tig0027457:+59072 Msa1434040:CDS
GCTAACTCGTGCAATGAACG+TGG 0.642463 tig0027457:+59129 Msa1434040:CDS
ACCCGGTTGTGCAAATGGAG+CGG 0.671005 tig0027457:+59299 Msa1434040:CDS
GTTAGCGAGTTAAGAGAACC+GGG 0.676058 tig0027457:-59112 None:intergenic
GCGAGTTAAGAGAACCGGGA+CGG 0.686101 tig0027457:-59108 None:intergenic
CGAACCAAACGGAATCGCGG+TGG 0.692597 tig0027457:+59075 Msa1434040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGCTTTCAAGAATCTAGAC+CGG + tig0027457:59161-59180 Msa1434040:CDS 35.0%
GTTCGGGTGATTATATTAAC+CGG + tig0027457:59193-59212 Msa1434040:CDS 35.0%
GGTGATTATATTAACCGGAA+CGG + tig0027457:59198-59217 Msa1434040:CDS 35.0%
GTGATTATATTAACCGGAAC+GGG + tig0027457:59199-59218 Msa1434040:CDS 35.0%
CCTTTAATCGCTCCAATTAT+CGG - tig0027457:59334-59353 None:intergenic 35.0%
!!! GATATTTTGGTTGCTGCTAA+AGG + tig0027457:59394-59413 Msa1434040:CDS 35.0%
AGTTAGCGAGTTAAGAGAAC+CGG - tig0027457:59116-59135 None:intergenic 40.0%
AGCTTTCAAGAATCTAGACC+GGG + tig0027457:59162-59181 Msa1434040:CDS 40.0%
!! TGTTTTCAAGGGTGATGTGA+AGG + tig0027457:59264-59283 Msa1434040:CDS 40.0%
CCGATAATTGGAGCGATTAA+AGG + tig0027457:59331-59350 Msa1434040:CDS 40.0%
! TGCGCTTGCTTGTGATATTT+TGG + tig0027457:59381-59400 Msa1434040:CDS 40.0%
TGCTAAAGGAGCCAAATTCA+TGG + tig0027457:59408-59427 Msa1434040:CDS 40.0%
AGCATGAGTGTCCATGAATT+TGG - tig0027457:59422-59441 None:intergenic 40.0%
GTTAGCGAGTTAAGAGAACC+GGG - tig0027457:59115-59134 None:intergenic 45.0%
GATTCTTGAAAGCTTGAGCC+AGG - tig0027457:59156-59175 None:intergenic 45.0%
!! CGGTGTCGTAAACCGATAAT+TGG + tig0027457:59319-59338 Msa1434040:CDS 45.0%
AAAGGATTCGCTGTTACAGC+TGG + tig0027457:59349-59368 Msa1434040:CDS 45.0%
GTGGTGACTATTAACCGTCC+CGG + tig0027457:59094-59113 Msa1434040:CDS 50.0%
GCTAACTCGTGCAATGAACG+TGG + tig0027457:59129-59148 Msa1434040:CDS 50.0%
GAATCTAGACCGGGACGAAT+CGG + tig0027457:59171-59190 Msa1434040:CDS 50.0%
ACAGAACGATCTACCCGTTC+CGG - tig0027457:59215-59234 None:intergenic 50.0%
ACGGGTAGATCGTTCTGTTC+CGG + tig0027457:59217-59236 Msa1434040:CDS 50.0%
! GCAGCGGAGGATGTTTTCAA+GGG + tig0027457:59253-59272 Msa1434040:CDS 50.0%
CAAGGGTGATGTGAAGGATC+CGG + tig0027457:59270-59289 Msa1434040:CDS 50.0%
CTCGTCAACCGCGAACCAAA+CGG + tig0027457:59064-59083 Msa1434040:CDS 55.0%
GCGAGTTAAGAGAACCGGGA+CGG - tig0027457:59111-59130 None:intergenic 55.0%
TCGTGCAATGAACGTGGACC+TGG + tig0027457:59135-59154 Msa1434040:CDS 55.0%
TAGACCGGGACGAATCGGTT+CGG + tig0027457:59176-59195 Msa1434040:CDS 55.0%
!! CTGTTCCGGCGTTGACCTTA+CGG + tig0027457:59231-59250 Msa1434040:CDS 55.0%
AAACATCCTCCGCTGCCGTA+AGG - tig0027457:59249-59268 None:intergenic 55.0%
! GGCAGCGGAGGATGTTTTCA+AGG + tig0027457:59252-59271 Msa1434040:CDS 55.0%
GAGTGACCCGGTTGTGCAAA+TGG + tig0027457:59294-59313 Msa1434040:CDS 55.0%
ACCCGGTTGTGCAAATGGAG+CGG + tig0027457:59299-59318 Msa1434040:CDS 55.0%
ACCGCTCCATTTGCACAACC+GGG - tig0027457:59303-59322 None:intergenic 55.0%
CACCGCTCCATTTGCACAAC+CGG - tig0027457:59304-59323 None:intergenic 55.0%
! CCGCGATTCCGTTTGGTTCG+CGG - tig0027457:59075-59094 None:intergenic 60.0%
CCGCGAACCAAACGGAATCG+CGG + tig0027457:59072-59091 Msa1434040:CDS 60.0%
CGAACCAAACGGAATCGCGG+TGG + tig0027457:59075-59094 Msa1434040:CDS 60.0%
GTCACCACCGCGATTCCGTT+TGG - tig0027457:59082-59101 None:intergenic 60.0%
! AGACCGGGACGAATCGGTTC+GGG + tig0027457:59177-59196 Msa1434040:CDS 60.0%
!! TCACCCGAACCGATTCGTCC+CGG - tig0027457:59183-59202 None:intergenic 60.0%
GAAGGATCCGGAGAGTGACC+CGG + tig0027457:59282-59301 Msa1434040:CDS 60.0%
TGCACAACCGGGTCACTCTC+CGG - tig0027457:59292-59311 None:intergenic 60.0%
CGCTGCCGTAAGGTCAACGC+CGG - tig0027457:59239-59258 None:intergenic 65.0%
!! CGGCGTTGACCTTACGGCAG+CGG + tig0027457:59237-59256 Msa1434040:CDS 65.0%
CGTTGACCTTACGGCAGCGG+AGG + tig0027457:59240-59259 Msa1434040:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0027457 gene 59043 59441 59043 ID=Msa1434040;Name=Msa1434040
tig0027457 mRNA 59043 59441 59043 ID=Msa1434040-mRNA-1;Parent=Msa1434040;Name=Msa1434040-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|133
tig0027457 exon 59043 59441 59043 ID=Msa1434040-mRNA-1:exon:8920;Parent=Msa1434040-mRNA-1
tig0027457 CDS 59043 59441 59043 ID=Msa1434040-mRNA-1:cds;Parent=Msa1434040-mRNA-1
Gene Sequence

>Msa1434040

ATGACATCGGAGAAGCTGATACTCGTCAACCGCGAACCAAACGGAATCGCGGTGGTGACTATTAACCGTCCCGGTTCTCTTAACTCGCTAACTCGTGCAATGAACGTGGACCTGGCTCAAGCTTTCAAGAATCTAGACCGGGACGAATCGGTTCGGGTGATTATATTAACCGGAACGGGTAGATCGTTCTGTTCCGGCGTTGACCTTACGGCAGCGGAGGATGTTTTCAAGGGTGATGTGAAGGATCCGGAGAGTGACCCGGTTGTGCAAATGGAGCGGTGTCGTAAACCGATAATTGGAGCGATTAAAGGATTCGCTGTTACAGCTGGATTTGAGATTGCGCTTGCTTGTGATATTTTGGTTGCTGCTAAAGGAGCCAAATTCATGGACACTCATGCT

Protein sequence

>Msa1434040

MTSEKLILVNREPNGIAVVTINRPGSLNSLTRAMNVDLAQAFKNLDRDESVRVIILTGTGRSFCSGVDLTAAEDVFKGDVKDPESDPVVQMERCRKPIIGAIKGFAVTAGFEIALACDILVAAKGAKFMDTHA