Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60454.t1 | AFK43236.1 | 98.6 | 138 | 2 | 0 | 98 | 235 | 95 | 232 | 5.90E-72 | 281.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60454.t1 | P82658 | 80.0 | 140 | 28 | 0 | 98 | 237 | 81 | 220 | 7.3e-63 | 242.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60454.t1 | G7IWL6 | 98.6 | 138 | 2 | 0 | 98 | 235 | 96 | 233 | 4.3e-72 | 281.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene60454 | 0.836133 | 1.12E-56 | -1.69E-46 |
| MS.gene049171 | MS.gene60454 | 0.842359 | 2.71E-58 | -1.69E-46 |
| MS.gene049173 | MS.gene60454 | 0.869625 | 2.69E-66 | -1.69E-46 |
| MS.gene049217 | MS.gene60454 | 0.866956 | 1.95E-65 | -1.69E-46 |
| MS.gene049222 | MS.gene60454 | 0.872238 | 3.71E-67 | -1.69E-46 |
| MS.gene049285 | MS.gene60454 | 0.869573 | 2.80E-66 | -1.69E-46 |
| MS.gene049320 | MS.gene60454 | 0.868567 | 5.93E-66 | -1.69E-46 |
| MS.gene049356 | MS.gene60454 | 0.804085 | 2.56E-49 | -1.69E-46 |
| MS.gene049369 | MS.gene60454 | 0.821184 | 4.62E-53 | -1.69E-46 |
| MS.gene04939 | MS.gene60454 | 0.811001 | 8.71E-51 | -1.69E-46 |
| MS.gene049427 | MS.gene60454 | 0.86022 | 2.40E-63 | -1.69E-46 |
| MS.gene049609 | MS.gene60454 | 0.848385 | 6.34E-60 | -1.69E-46 |
| MS.gene049614 | MS.gene60454 | 0.811946 | 5.43E-51 | -1.69E-46 |
| MS.gene049615 | MS.gene60454 | 0.802432 | 5.63E-49 | -1.69E-46 |
| MS.gene049661 | MS.gene60454 | 0.805894 | 1.07E-49 | -1.69E-46 |
| MS.gene049805 | MS.gene60454 | 0.865209 | 6.97E-65 | -1.69E-46 |
| MS.gene050076 | MS.gene60454 | 0.847322 | 1.24E-59 | -1.69E-46 |
| MS.gene050163 | MS.gene60454 | 0.875775 | 2.36E-68 | -1.69E-46 |
| MS.gene050165 | MS.gene60454 | 0.852701 | 3.88E-61 | -1.69E-46 |
| MS.gene050333 | MS.gene60454 | 0.847317 | 1.25E-59 | -1.69E-46 |
| MS.gene050432 | MS.gene60454 | 0.835776 | 1.38E-56 | -1.69E-46 |
| MS.gene050444 | MS.gene60454 | 0.824378 | 8.34E-54 | -1.69E-46 |
| MS.gene050504 | MS.gene60454 | 0.838618 | 2.58E-57 | -1.69E-46 |
| MS.gene050518 | MS.gene60454 | 0.833293 | 5.80E-56 | -1.69E-46 |
| MS.gene050538 | MS.gene60454 | 0.823172 | 1.60E-53 | -1.69E-46 |
| MS.gene050577 | MS.gene60454 | 0.819859 | 9.31E-53 | -1.69E-46 |
| MS.gene050593 | MS.gene60454 | 0.833826 | 4.27E-56 | -1.69E-46 |
| MS.gene050612 | MS.gene60454 | 0.826035 | 3.38E-54 | -1.69E-46 |
| MS.gene050675 | MS.gene60454 | 0.817403 | 3.36E-52 | -1.69E-46 |
| MS.gene050686 | MS.gene60454 | 0.816192 | 6.28E-52 | -1.69E-46 |
| MS.gene050699 | MS.gene60454 | 0.843751 | 1.16E-58 | -1.69E-46 |
| MS.gene050747 | MS.gene60454 | 0.849491 | 3.13E-60 | -1.69E-46 |
| MS.gene050767 | MS.gene60454 | 0.856579 | 2.93E-62 | -1.69E-46 |
| MS.gene050770 | MS.gene60454 | 0.85633 | 3.46E-62 | -1.69E-46 |
| MS.gene050772 | MS.gene60454 | 0.87023 | 1.71E-66 | -1.69E-46 |
| MS.gene050781 | MS.gene60454 | 0.8068 | 6.90E-50 | -1.69E-46 |
| MS.gene050783 | MS.gene60454 | 0.807719 | 4.41E-50 | -1.69E-46 |
| MS.gene05080 | MS.gene60454 | 0.806097 | 9.71E-50 | -1.69E-46 |
| MS.gene050812 | MS.gene60454 | 0.802754 | 4.83E-49 | -1.69E-46 |
| MS.gene050950 | MS.gene60454 | 0.83166 | 1.47E-55 | -1.69E-46 |
| MS.gene050986 | MS.gene60454 | 0.814709 | 1.34E-51 | -1.69E-46 |
| MS.gene05100 | MS.gene60454 | 0.903406 | 3.58E-79 | -1.69E-46 |
| MS.gene05101 | MS.gene60454 | 0.816118 | 6.52E-52 | -1.69E-46 |
| MS.gene05105 | MS.gene60454 | 0.842906 | 1.94E-58 | -1.69E-46 |
| MS.gene051228 | MS.gene60454 | 0.846379 | 2.25E-59 | -1.69E-46 |
| MS.gene051387 | MS.gene60454 | 0.829502 | 4.97E-55 | -1.69E-46 |
| MS.gene051551 | MS.gene60454 | 0.835461 | 1.66E-56 | -1.69E-46 |
| MS.gene051666 | MS.gene60454 | 0.885569 | 7.27E-72 | -1.69E-46 |
| MS.gene051692 | MS.gene60454 | 0.84377 | 1.14E-58 | -1.69E-46 |
| MS.gene051694 | MS.gene60454 | 0.80278 | 4.77E-49 | -1.69E-46 |
| MS.gene051810 | MS.gene60454 | 0.834205 | 3.43E-56 | -1.69E-46 |
| MS.gene051858 | MS.gene60454 | 0.840591 | 7.93E-58 | -1.69E-46 |
| MS.gene051949 | MS.gene60454 | 0.816744 | 4.72E-52 | -1.69E-46 |
| MS.gene052164 | MS.gene60454 | 0.822027 | 2.95E-53 | -1.69E-46 |
| MS.gene052271 | MS.gene60454 | 0.882465 | 1.02E-70 | -1.69E-46 |
| MS.gene052290 | MS.gene60454 | 0.840898 | 6.59E-58 | -1.69E-46 |
| MS.gene052310 | MS.gene60454 | 0.81883 | 1.60E-52 | -1.69E-46 |
| MS.gene052379 | MS.gene60454 | 0.853855 | 1.81E-61 | -1.69E-46 |
| MS.gene052421 | MS.gene60454 | 0.846713 | 1.83E-59 | -1.69E-46 |
| MS.gene052481 | MS.gene60454 | 0.822051 | 2.91E-53 | -1.69E-46 |
| MS.gene052484 | MS.gene60454 | 0.828106 | 1.08E-54 | -1.69E-46 |
| MS.gene052485 | MS.gene60454 | 0.802492 | 5.47E-49 | -1.69E-46 |
| MS.gene052486 | MS.gene60454 | 0.800623 | 1.32E-48 | -1.69E-46 |
| MS.gene052501 | MS.gene60454 | 0.838213 | 3.29E-57 | -1.69E-46 |
| MS.gene052503 | MS.gene60454 | 0.844961 | 5.47E-59 | -1.69E-46 |
| MS.gene052505 | MS.gene60454 | 0.816875 | 4.41E-52 | -1.69E-46 |
| MS.gene052615 | MS.gene60454 | 0.842181 | 3.02E-58 | -1.69E-46 |
| MS.gene052670 | MS.gene60454 | 0.803042 | 4.21E-49 | -1.69E-46 |
| MS.gene052823 | MS.gene60454 | 0.832962 | 7.01E-56 | -1.69E-46 |
| MS.gene052825 | MS.gene60454 | 0.829085 | 6.27E-55 | -1.69E-46 |
| MS.gene052872 | MS.gene60454 | 0.80336 | 3.62E-49 | -1.69E-46 |
| MS.gene052916 | MS.gene60454 | 0.820547 | 6.48E-53 | -1.69E-46 |
| MS.gene053009 | MS.gene60454 | 0.803938 | 2.75E-49 | -1.69E-46 |
| MS.gene053103 | MS.gene60454 | 0.86735 | 1.46E-65 | -1.69E-46 |
| MS.gene053104 | MS.gene60454 | 0.882243 | 1.23E-70 | -1.69E-46 |
| MS.gene053207 | MS.gene60454 | 0.833376 | 5.53E-56 | -1.69E-46 |
| MS.gene053213 | MS.gene60454 | 0.881265 | 2.77E-70 | -1.69E-46 |
| MS.gene053286 | MS.gene60454 | 0.871392 | 7.08E-67 | -1.69E-46 |
| MS.gene053322 | MS.gene60454 | 0.880979 | 3.51E-70 | -1.69E-46 |
| MS.gene053436 | MS.gene60454 | 0.83936 | 1.66E-57 | -1.69E-46 |
| MS.gene053449 | MS.gene60454 | 0.803523 | 3.35E-49 | -1.69E-46 |
| MS.gene053574 | MS.gene60454 | 0.850864 | 1.29E-60 | -1.69E-46 |
| MS.gene053632 | MS.gene60454 | 0.888806 | 4.26E-73 | -1.69E-46 |
| MS.gene053813 | MS.gene60454 | 0.811887 | 5.59E-51 | -1.69E-46 |
| MS.gene053824 | MS.gene60454 | 0.811963 | 5.38E-51 | -1.69E-46 |
| MS.gene053915 | MS.gene60454 | 0.807046 | 6.12E-50 | -1.69E-46 |
| MS.gene053916 | MS.gene60454 | 0.842876 | 1.98E-58 | -1.69E-46 |
| MS.gene054376 | MS.gene60454 | 0.83641 | 9.51E-57 | -1.69E-46 |
| MS.gene054408 | MS.gene60454 | 0.824748 | 6.82E-54 | -1.69E-46 |
| MS.gene054595 | MS.gene60454 | 0.825669 | 4.13E-54 | -1.69E-46 |
| MS.gene054600 | MS.gene60454 | 0.858917 | 5.93E-63 | -1.69E-46 |
| MS.gene054642 | MS.gene60454 | 0.877963 | 4.12E-69 | -1.69E-46 |
| MS.gene054802 | MS.gene60454 | 0.837613 | 4.69E-57 | -1.69E-46 |
| MS.gene054803 | MS.gene60454 | 0.833469 | 5.24E-56 | -1.69E-46 |
| MS.gene054804 | MS.gene60454 | 0.838742 | 2.40E-57 | -1.69E-46 |
| MS.gene054927 | MS.gene60454 | 0.815729 | 7.96E-52 | -1.69E-46 |
| MS.gene054954 | MS.gene60454 | 0.833712 | 4.56E-56 | -1.69E-46 |
| MS.gene05505 | MS.gene60454 | 0.870887 | 1.04E-66 | -1.69E-46 |
| MS.gene055116 | MS.gene60454 | 0.806866 | 6.68E-50 | -1.69E-46 |
| MS.gene055154 | MS.gene60454 | -0.804057 | 2.59E-49 | -1.69E-46 |
| MS.gene055206 | MS.gene60454 | 0.839931 | 1.18E-57 | -1.69E-46 |
| MS.gene055212 | MS.gene60454 | 0.872712 | 2.58E-67 | -1.69E-46 |
| MS.gene055254 | MS.gene60454 | 0.808243 | 3.41E-50 | -1.69E-46 |
| MS.gene055289 | MS.gene60454 | 0.824069 | 9.85E-54 | -1.69E-46 |
| MS.gene055362 | MS.gene60454 | 0.865024 | 7.97E-65 | -1.69E-46 |
| MS.gene055397 | MS.gene60454 | 0.870002 | 2.03E-66 | -1.69E-46 |
| MS.gene055412 | MS.gene60454 | 0.81086 | 9.34E-51 | -1.69E-46 |
| MS.gene055499 | MS.gene60454 | 0.866484 | 2.76E-65 | -1.69E-46 |
| MS.gene055502 | MS.gene60454 | 0.863966 | 1.71E-64 | -1.69E-46 |
| MS.gene055577 | MS.gene60454 | 0.82488 | 6.35E-54 | -1.69E-46 |
| MS.gene055636 | MS.gene60454 | 0.81712 | 3.89E-52 | -1.69E-46 |
| MS.gene055638 | MS.gene60454 | 0.821778 | 3.37E-53 | -1.69E-46 |
| MS.gene055647 | MS.gene60454 | 0.857413 | 1.66E-62 | -1.69E-46 |
| MS.gene055722 | MS.gene60454 | 0.872352 | 3.40E-67 | -1.69E-46 |
| MS.gene055748 | MS.gene60454 | 0.801226 | 9.95E-49 | -1.69E-46 |
| MS.gene055750 | MS.gene60454 | 0.817536 | 3.13E-52 | -1.69E-46 |
| MS.gene05592 | MS.gene60454 | 0.843824 | 1.10E-58 | -1.69E-46 |
| MS.gene055941 | MS.gene60454 | 0.858632 | 7.21E-63 | -1.69E-46 |
| MS.gene055956 | MS.gene60454 | -0.809444 | 1.89E-50 | -1.69E-46 |
| MS.gene056083 | MS.gene60454 | 0.814272 | 1.68E-51 | -1.69E-46 |
| MS.gene056374 | MS.gene60454 | 0.874385 | 7.04E-68 | -1.69E-46 |
| MS.gene056410 | MS.gene60454 | 0.807416 | 5.11E-50 | -1.69E-46 |
| MS.gene056422 | MS.gene60454 | 0.801841 | 7.44E-49 | -1.69E-46 |
| MS.gene056449 | MS.gene60454 | 0.805957 | 1.04E-49 | -1.69E-46 |
| MS.gene056487 | MS.gene60454 | 0.830388 | 3.02E-55 | -1.69E-46 |
| MS.gene056504 | MS.gene60454 | 0.815327 | 9.79E-52 | -1.69E-46 |
| MS.gene056510 | MS.gene60454 | 0.814035 | 1.89E-51 | -1.69E-46 |
| MS.gene056511 | MS.gene60454 | 0.814627 | 1.40E-51 | -1.69E-46 |
| MS.gene056549 | MS.gene60454 | 0.825649 | 4.18E-54 | -1.69E-46 |
| MS.gene056609 | MS.gene60454 | 0.837221 | 5.91E-57 | -1.69E-46 |
| MS.gene05664 | MS.gene60454 | 0.804232 | 2.38E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene60454 | 0.897473 | 1.36E-76 | -1.69E-46 |
| MS.gene056897 | MS.gene60454 | 0.809536 | 1.80E-50 | -1.69E-46 |
| MS.gene056942 | MS.gene60454 | 0.817423 | 3.32E-52 | -1.69E-46 |
| MS.gene057011 | MS.gene60454 | 0.823776 | 1.15E-53 | -1.69E-46 |
| MS.gene057048 | MS.gene60454 | 0.800184 | 1.62E-48 | -1.69E-46 |
| MS.gene057076 | MS.gene60454 | 0.831186 | 1.92E-55 | -1.69E-46 |
| MS.gene057123 | MS.gene60454 | 0.812243 | 4.68E-51 | -1.69E-46 |
| MS.gene057129 | MS.gene60454 | 0.885853 | 5.68E-72 | -1.69E-46 |
| MS.gene057146 | MS.gene60454 | 0.853363 | 2.51E-61 | -1.69E-46 |
| MS.gene057295 | MS.gene60454 | 0.82893 | 6.84E-55 | -1.69E-46 |
| MS.gene057331 | MS.gene60454 | 0.82084 | 5.54E-53 | -1.69E-46 |
| MS.gene057416 | MS.gene60454 | 0.869903 | 2.18E-66 | -1.69E-46 |
| MS.gene057658 | MS.gene60454 | 0.822835 | 1.91E-53 | -1.69E-46 |
| MS.gene057734 | MS.gene60454 | 0.804733 | 1.87E-49 | -1.69E-46 |
| MS.gene057823 | MS.gene60454 | 0.802724 | 4.90E-49 | -1.69E-46 |
| MS.gene057831 | MS.gene60454 | 0.828914 | 6.90E-55 | -1.69E-46 |
| MS.gene057926 | MS.gene60454 | 0.842182 | 3.02E-58 | -1.69E-46 |
| MS.gene058019 | MS.gene60454 | 0.907723 | 3.72E-81 | -1.69E-46 |
| MS.gene058293 | MS.gene60454 | 0.847221 | 1.33E-59 | -1.69E-46 |
| MS.gene058323 | MS.gene60454 | 0.806815 | 6.85E-50 | -1.69E-46 |
| MS.gene058338 | MS.gene60454 | 0.812359 | 4.41E-51 | -1.69E-46 |
| MS.gene058419 | MS.gene60454 | 0.888711 | 4.63E-73 | -1.69E-46 |
| MS.gene058544 | MS.gene60454 | 0.839354 | 1.67E-57 | -1.69E-46 |
| MS.gene058660 | MS.gene60454 | 0.845226 | 4.63E-59 | -1.69E-46 |
| MS.gene058723 | MS.gene60454 | 0.83958 | 1.46E-57 | -1.69E-46 |
| MS.gene058773 | MS.gene60454 | 0.820227 | 7.67E-53 | -1.69E-46 |
| MS.gene058838 | MS.gene60454 | 0.83233 | 1.01E-55 | -1.69E-46 |
| MS.gene059022 | MS.gene60454 | 0.837052 | 6.52E-57 | -1.69E-46 |
| MS.gene059023 | MS.gene60454 | 0.811471 | 6.89E-51 | -1.69E-46 |
| MS.gene059029 | MS.gene60454 | 0.846997 | 1.53E-59 | -1.69E-46 |
| MS.gene05913 | MS.gene60454 | 0.86495 | 8.41E-65 | -1.69E-46 |
| MS.gene05934 | MS.gene60454 | 0.867858 | 1.00E-65 | -1.69E-46 |
| MS.gene059367 | MS.gene60454 | 0.836757 | 7.76E-57 | -1.69E-46 |
| MS.gene059368 | MS.gene60454 | 0.816856 | 4.46E-52 | -1.69E-46 |
| MS.gene059455 | MS.gene60454 | 0.833166 | 6.23E-56 | -1.69E-46 |
| MS.gene059536 | MS.gene60454 | 0.870078 | 1.91E-66 | -1.69E-46 |
| MS.gene059597 | MS.gene60454 | 0.820113 | 8.14E-53 | -1.69E-46 |
| MS.gene059676 | MS.gene60454 | 0.815865 | 7.43E-52 | -1.69E-46 |
| MS.gene059677 | MS.gene60454 | 0.858646 | 7.14E-63 | -1.69E-46 |
| MS.gene059688 | MS.gene60454 | 0.835148 | 1.99E-56 | -1.69E-46 |
| MS.gene059692 | MS.gene60454 | 0.836406 | 9.53E-57 | -1.69E-46 |
| MS.gene059695 | MS.gene60454 | 0.844279 | 8.34E-59 | -1.69E-46 |
| MS.gene059711 | MS.gene60454 | 0.813414 | 2.59E-51 | -1.69E-46 |
| MS.gene059718 | MS.gene60454 | 0.857933 | 1.16E-62 | -1.69E-46 |
| MS.gene059756 | MS.gene60454 | 0.828081 | 1.10E-54 | -1.69E-46 |
| MS.gene059801 | MS.gene60454 | 0.817034 | 4.06E-52 | -1.69E-46 |
| MS.gene059858 | MS.gene60454 | 0.829357 | 5.39E-55 | -1.69E-46 |
| MS.gene059865 | MS.gene60454 | 0.806038 | 9.99E-50 | -1.69E-46 |
| MS.gene059902 | MS.gene60454 | 0.834844 | 2.37E-56 | -1.69E-46 |
| MS.gene059960 | MS.gene60454 | 0.885736 | 6.29E-72 | -1.69E-46 |
| MS.gene060193 | MS.gene60454 | 0.81807 | 2.37E-52 | -1.69E-46 |
| MS.gene060341 | MS.gene60454 | 0.804988 | 1.66E-49 | -1.69E-46 |
| MS.gene060403 | MS.gene60454 | 0.803067 | 4.16E-49 | -1.69E-46 |
| MS.gene060482 | MS.gene60454 | 0.823894 | 1.08E-53 | -1.69E-46 |
| MS.gene060703 | MS.gene60454 | 0.829826 | 4.14E-55 | -1.69E-46 |
| MS.gene060727 | MS.gene60454 | 0.808575 | 2.90E-50 | -1.69E-46 |
| MS.gene060762 | MS.gene60454 | 0.821705 | 3.50E-53 | -1.69E-46 |
| MS.gene06077 | MS.gene60454 | 0.817667 | 2.93E-52 | -1.69E-46 |
| MS.gene060804 | MS.gene60454 | 0.899568 | 1.74E-77 | -1.69E-46 |
| MS.gene060818 | MS.gene60454 | 0.843817 | 1.11E-58 | -1.69E-46 |
| MS.gene060835 | MS.gene60454 | 0.84692 | 1.60E-59 | -1.69E-46 |
| MS.gene060837 | MS.gene60454 | 0.815916 | 7.23E-52 | -1.69E-46 |
| MS.gene06092 | MS.gene60454 | 0.829945 | 3.88E-55 | -1.69E-46 |
| MS.gene06097 | MS.gene60454 | 0.802328 | 5.91E-49 | -1.69E-46 |
| MS.gene061041 | MS.gene60454 | 0.89646 | 3.61E-76 | -1.69E-46 |
| MS.gene061051 | MS.gene60454 | -0.807271 | 5.48E-50 | -1.69E-46 |
| MS.gene061150 | MS.gene60454 | 0.842948 | 1.89E-58 | -1.69E-46 |
| MS.gene061200 | MS.gene60454 | 0.900964 | 4.32E-78 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60454.t1 | MTR_3g028150 | 96.170 | 235 | 7 | 2 | 1 | 235 | 1 | 233 | 7.83e-163 | 453 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60454.t1 | AT3G63540 | 79.545 | 132 | 27 | 0 | 106 | 237 | 3 | 134 | 1.38e-77 | 233 |
Find 68 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATATTTAACTTTCCTTTC+TGG | 0.121727 | 3.2:-19594863 | MS.gene60454:CDS |
| TCTTTCTCTGTTCTCTTCTT+TGG | 0.152693 | 3.2:+19594884 | None:intergenic |
| GGTGGAGGAGGAGGAGAAAA+TGG | 0.215436 | 3.2:+19595249 | None:intergenic |
| GGTGGTGGTGCTGGTGGAGG+AGG | 0.233443 | 3.2:+19595237 | None:intergenic |
| AAACGTTCCTTCCAACCTTC+TGG | 0.272048 | 3.2:+19594959 | None:intergenic |
| AATATTTAACTTTCCTTTCT+GGG | 0.289515 | 3.2:-19594862 | MS.gene60454:CDS |
| TTAATATCAAAAGTTGAAAA+AGG | 0.291067 | 3.2:-19594938 | MS.gene60454:CDS |
| TTCTGCTGAGTCTTTCTTGT+TGG | 0.307382 | 3.2:+19594997 | None:intergenic |
| AAAGATTTCAATGTGGGAAT+TGG | 0.309517 | 3.2:-19594277 | MS.gene60454:CDS |
| GGTGGTGCTGGTGGAGGAGG+AGG | 0.318310 | 3.2:+19595240 | None:intergenic |
| GGTGGTGGTGGTGCTGGTGG+AGG | 0.347609 | 3.2:+19595234 | None:intergenic |
| TGTGGTGGTTGTTGTGGTGG+TGG | 0.368516 | 3.2:+19595219 | None:intergenic |
| GGTGGTTGTGGTGGTTGTTG+TGG | 0.401207 | 3.2:+19595213 | None:intergenic |
| CAATTTCAATATCAACAGTC+TGG | 0.404205 | 3.2:+19594164 | None:intergenic |
| CCACATTGAAATCTTTAAAC+CGG | 0.413073 | 3.2:+19594314 | None:intergenic |
| GTTAAGGTGGTGGTTATTAG+AGG | 0.416358 | 3.2:+19595151 | None:intergenic |
| TTGTTCAAGACAGCGTCTTT+TGG | 0.421438 | 3.2:+19594824 | None:intergenic |
| GGTTGTGGTGGTTGTTGTGG+TGG | 0.423034 | 3.2:+19595216 | None:intergenic |
| GGTGGTTGTTGTGGTGGTGG+TGG | 0.425792 | 3.2:+19595222 | None:intergenic |
| TTATTTGTCTCACATTGCTC+AGG | 0.435723 | 3.2:-19594190 | MS.gene60454:CDS |
| TTTCAATGTGGGAATTGAAC+GGG | 0.448126 | 3.2:-19594302 | MS.gene60454:CDS |
| TATTATGTTTATGAGATTGA+TGG | 0.453881 | 3.2:-19594710 | MS.gene60454:CDS |
| GTGGAGGAGGAGGAGAAAAT+GGG | 0.454320 | 3.2:+19595250 | None:intergenic |
| GTGAGGGTGGATGTTGAAGT+TGG | 0.468165 | 3.2:-19594235 | MS.gene60454:CDS |
| TGTACCGTAGTAGAGATTGT+AGG | 0.471430 | 3.2:+19595060 | None:intergenic |
| GGGAGATGATTCTGCTGCTA+TGG | 0.482398 | 3.2:+19595081 | None:intergenic |
| GAAGAAGTGAAAGATGATAA+AGG | 0.482410 | 3.2:-19594740 | MS.gene60454:CDS |
| TCTTTATTCCACTCAGGAGT+AGG | 0.485770 | 3.2:+19594617 | None:intergenic |
| GCAGCAAACTACGGCGGCTA+CGG | 0.501712 | 3.2:-19595028 | MS.gene60454:CDS |
| AGGTGGTGATGTTGTGAGAA+TGG | 0.508602 | 3.2:+19595105 | None:intergenic |
| TGATATGGTGGTGGTTGTGG+TGG | 0.513282 | 3.2:+19595204 | None:intergenic |
| AATTTGCAAGATATTATTAG+TGG | 0.515902 | 3.2:-19594782 | MS.gene60454:CDS |
| TCCTTATCTTTATTCCACTC+AGG | 0.519893 | 3.2:+19594611 | None:intergenic |
| ATCCACCCTCACGCCTAGCG+TGG | 0.520319 | 3.2:+19594246 | None:intergenic |
| TTGTGTTTGAATGAGATAAG+AGG | 0.520933 | 3.2:+19595178 | None:intergenic |
| CGACGTTCCAGAAGGTTGGA+AGG | 0.523300 | 3.2:-19594966 | MS.gene60454:CDS |
| CCTCCCGCTTAAAACTCATT+CGG | 0.523545 | 3.2:-19594122 | MS.gene60454:CDS |
| TGCTGCTGCTGTTAAGGTGG+TGG | 0.525546 | 3.2:+19595141 | None:intergenic |
| TCCTGAGTGGAATAAAGATA+AGG | 0.526998 | 3.2:-19594612 | MS.gene60454:CDS |
| TTTACGACGTTCCAGAAGGT+TGG | 0.527621 | 3.2:-19594970 | MS.gene60454:CDS |
| CGGTTTAAAGATTTCAATGT+GGG | 0.529670 | 3.2:-19594313 | MS.gene60454:CDS |
| TCAGGCCCACGCTAGGCGTG+AGG | 0.536275 | 3.2:-19594252 | MS.gene60454:CDS |
| ATTGGACTCAGGCCCACGCT+AGG | 0.544401 | 3.2:-19594259 | MS.gene60454:CDS |
| TGCTGCTGCTGCTGTTAAGG+TGG | 0.548707 | 3.2:+19595138 | None:intergenic |
| GAGGAGGAGGAGAAAATGGG+TGG | 0.548714 | 3.2:+19595253 | None:intergenic |
| GTACCGTAGTAGAGATTGTA+GGG | 0.551840 | 3.2:+19595061 | None:intergenic |
| TCAATGTGGGAATTGGACTC+AGG | 0.555453 | 3.2:-19594270 | MS.gene60454:CDS |
| ATTTCAATGTGGGAATTGAA+CGG | 0.557096 | 3.2:-19594303 | MS.gene60454:CDS |
| GCCCACGCTAGGCGTGAGGG+TGG | 0.558340 | 3.2:-19594248 | MS.gene60454:CDS |
| CGGGCTAAAGATTTCAATGT+GGG | 0.568974 | 3.2:-19594283 | MS.gene60454:CDS |
| GAGGAGGAGAAAATGGGTGG+TGG | 0.570174 | 3.2:+19595256 | None:intergenic |
| CAGGCCCACGCTAGGCGTGA+GGG | 0.573779 | 3.2:-19594251 | MS.gene60454:CDS |
| TGTTGATATTGAAATTGTGA+TGG | 0.582207 | 3.2:-19594158 | MS.gene60454:CDS |
| GATGATTCTGCTGCTATGGA+AGG | 0.583786 | 3.2:+19595085 | None:intergenic |
| AATTTCAATATCAACAGTCT+GGG | 0.591228 | 3.2:+19594165 | None:intergenic |
| GCAGCAAGTGCAGCAAACTA+CGG | 0.594999 | 3.2:-19595037 | MS.gene60454:CDS |
| TCCACCCTCACGCCTAGCGT+GGG | 0.596178 | 3.2:+19594247 | None:intergenic |
| TACATTTACGACGTTCCAGA+AGG | 0.599346 | 3.2:-19594974 | MS.gene60454:CDS |
| TCTCCCTACAATCTCTACTA+CGG | 0.607493 | 3.2:-19595064 | MS.gene60454:CDS |
| GCAAACTACGGCGGCTACGG+CGG | 0.613621 | 3.2:-19595025 | MS.gene60454:CDS |
| GCAAGTGCAGCAAACTACGG+CGG | 0.619443 | 3.2:-19595034 | MS.gene60454:CDS |
| CTAACTGACGAAACCCAGAA+AGG | 0.634618 | 3.2:+19594849 | None:intergenic |
| TTAATGCTCCTACTCCTGAG+TGG | 0.644053 | 3.2:-19594625 | MS.gene60454:CDS |
| GATTCTGCTGCTATGGAAGG+TGG | 0.667537 | 3.2:+19595088 | None:intergenic |
| CCGGTTTAAAGATTTCAATG+TGG | 0.678539 | 3.2:-19594314 | MS.gene60454:CDS |
| TGGTGATGTTGTGAGAATGG+TGG | 0.683595 | 3.2:+19595108 | None:intergenic |
| TGGTGATGATGAGAAAATTG+TGG | 0.687506 | 3.2:+19595276 | None:intergenic |
| ACGGGCTAAAGATTTCAATG+TGG | 0.712707 | 3.2:-19594284 | MS.gene60454:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATATCAAAAGTTGAAAA+AGG | - | chr3.2:19594447-19594466 | MS.gene60454:intron | 15.0% |
| !! | AATTTGCAAGATATTATTAG+TGG | - | chr3.2:19594603-19594622 | MS.gene60454:CDS | 20.0% |
| !! | TATTATGTTTATGAGATTGA+TGG | - | chr3.2:19594675-19594694 | MS.gene60454:CDS | 20.0% |
| !!! | AATATGGTTTTGTTTAACAA+TGG | - | chr3.2:19594873-19594892 | MS.gene60454:CDS | 20.0% |
| !!! | AATATTTAACTTTCCTTTCT+GGG | - | chr3.2:19594523-19594542 | MS.gene60454:intron | 20.0% |
| !!! | ATATTCTATGATAATGAGAA+TGG | - | chr3.2:19594896-19594915 | MS.gene60454:CDS | 20.0% |
| ! | AATTTCAATATCAACAGTCT+GGG | + | chr3.2:19595223-19595242 | None:intergenic | 25.0% |
| ! | TGTTGATATTGAAATTGTGA+TGG | - | chr3.2:19595227-19595246 | MS.gene60454:CDS | 25.0% |
| !! | AAAGTTGAAAAAGGTACAAA+TGG | - | chr3.2:19594456-19594475 | MS.gene60454:intron | 25.0% |
| !! | GAATATTTAACTTTCCTTTC+TGG | - | chr3.2:19594522-19594541 | MS.gene60454:intron | 25.0% |
| AAAGATTTCAATGTGGGAAT+TGG | - | chr3.2:19595078-19595097 | MS.gene60454:CDS | 30.0% | |
| ATTTAACATGATATCAGAGC+CGG | - | chr3.2:19595052-19595071 | MS.gene60454:CDS | 30.0% | |
| ATTTCAATGTGGGAATTGAA+CGG | - | chr3.2:19595082-19595101 | MS.gene60454:CDS | 30.0% | |
| CAATTTCAATATCAACAGTC+TGG | + | chr3.2:19595224-19595243 | None:intergenic | 30.0% | |
| CCACATTGAAATCTTTAAAC+CGG | + | chr3.2:19595074-19595093 | None:intergenic | 30.0% | |
| CGGTTTAAAGATTTCAATGT+GGG | - | chr3.2:19595072-19595091 | MS.gene60454:CDS | 30.0% | |
| GAAGAAGTGAAAGATGATAA+AGG | - | chr3.2:19594645-19594664 | MS.gene60454:CDS | 30.0% | |
| TATCTCAAAAGCTAACTCAA+AGG | + | chr3.2:19595020-19595039 | None:intergenic | 30.0% | |
| TATTTATACACTCAACAGCT+TGG | + | chr3.2:19594980-19594999 | None:intergenic | 30.0% | |
| TTATTTGTCTCATATTGCTC+AGG | - | chr3.2:19594951-19594970 | MS.gene60454:CDS | 30.0% | |
| TTGTGTTTGAATGAGATAAG+AGG | + | chr3.2:19594210-19594229 | None:intergenic | 30.0% | |
| !! | TGAGATAAGAGGTTTTGATA+TGG | + | chr3.2:19594199-19594218 | None:intergenic | 30.0% |
| CCGGTTTAAAGATTTCAATG+TGG | - | chr3.2:19595071-19595090 | MS.gene60454:CDS | 35.0% | |
| TCCTGAGTGGAATAAAGATA+AGG | - | chr3.2:19594773-19594792 | MS.gene60454:CDS | 35.0% | |
| TCCTTATCTTTATTCCACTC+AGG | + | chr3.2:19594777-19594796 | None:intergenic | 35.0% | |
| TCTTTCTCTGTTCTCTTCTT+TGG | + | chr3.2:19594504-19594523 | None:intergenic | 35.0% | |
| TTATTTGTCTCACATTGCTC+AGG | - | chr3.2:19595195-19595214 | MS.gene60454:CDS | 35.0% | |
| TTTCAATGTGGGAATTGAAC+GGG | - | chr3.2:19595083-19595102 | MS.gene60454:CDS | 35.0% | |
| ! | TGGTGATGATGAGAAAATTG+TGG | + | chr3.2:19594112-19594131 | None:intergenic | 35.0% |
| !! | GATAAGAGGTTTTGATATGG+TGG | + | chr3.2:19594196-19594215 | None:intergenic | 35.0% |
| !!! | AATGCCGAATGAGTTTTAAG+CGG | + | chr3.2:19595270-19595289 | None:intergenic | 35.0% |
| CGGGCTAAAGATTTCAATGT+GGG | - | chr3.2:19595102-19595121 | MS.gene60454:CDS | 40.0% | |
| GTACCGTAGTAGAGATTGTA+GGG | + | chr3.2:19594327-19594346 | None:intergenic | 40.0% | |
| TACATTTACGACGTTCCAGA+AGG | - | chr3.2:19594411-19594430 | MS.gene60454:intron | 40.0% | |
| TCTCCCTACAATCTCTACTA+CGG | - | chr3.2:19594321-19594340 | MS.gene60454:intron | 40.0% | |
| TCTTTATTCCACTCAGGAGT+AGG | + | chr3.2:19594771-19594790 | None:intergenic | 40.0% | |
| TGTACCGTAGTAGAGATTGT+AGG | + | chr3.2:19594328-19594347 | None:intergenic | 40.0% | |
| TTCTGCTGAGTCTTTCTTGT+TGG | + | chr3.2:19594391-19594410 | None:intergenic | 40.0% | |
| ! | ACGGGCTAAAGATTTCAATG+TGG | - | chr3.2:19595101-19595120 | MS.gene60454:CDS | 40.0% |
| ! | GTTAAGGTGGTGGTTATTAG+AGG | + | chr3.2:19594237-19594256 | None:intergenic | 40.0% |
| ! | TTGTTCAAGACAGCGTCTTT+TGG | + | chr3.2:19594564-19594583 | None:intergenic | 40.0% |
| !! | AAGAGGTTTTGATATGGTGG+TGG | + | chr3.2:19594193-19594212 | None:intergenic | 40.0% |
| !!! | ATGCCGAATGAGTTTTAAGC+GGG | + | chr3.2:19595269-19595288 | None:intergenic | 40.0% |
| !!! | TTTTGATATGGTGGTGGTTG+TGG | + | chr3.2:19594187-19594206 | None:intergenic | 40.0% |
| AAACGTTCCTTCCAACCTTC+TGG | + | chr3.2:19594429-19594448 | None:intergenic | 45.0% | |
| CCTCCCGCTTAAAACTCATT+CGG | - | chr3.2:19595263-19595282 | MS.gene60454:CDS | 45.0% | |
| CTAACTGACGAAACCCAGAA+AGG | + | chr3.2:19594539-19594558 | None:intergenic | 45.0% | |
| GATGATTCTGCTGCTATGGA+AGG | + | chr3.2:19594303-19594322 | None:intergenic | 45.0% | |
| TCAATGTGGGAATTGGACTC+AGG | - | chr3.2:19595115-19595134 | MS.gene60454:CDS | 45.0% | |
| TTAATGCTCCTACTCCTGAG+TGG | - | chr3.2:19594760-19594779 | MS.gene60454:CDS | 45.0% | |
| TTTACGACGTTCCAGAAGGT+TGG | - | chr3.2:19594415-19594434 | MS.gene60454:intron | 45.0% | |
| ! | AGGTGGTGATGTTGTGAGAA+TGG | + | chr3.2:19594283-19594302 | None:intergenic | 45.0% |
| ! | TGGTGATGTTGTGAGAATGG+TGG | + | chr3.2:19594280-19594299 | None:intergenic | 45.0% |
| !!! | AGTTTTAAGCGGGAGGTCTA+GGG | + | chr3.2:19595259-19595278 | None:intergenic | 45.0% |
| !!! | TTTTTGATATATAAATAATA+TGG | - | chr3.2:19594857-19594876 | MS.gene60454:CDS | 5.0% |
| GATTCTGCTGCTATGGAAGG+TGG | + | chr3.2:19594300-19594319 | None:intergenic | 50.0% | |
| GCAGCAAGTGCAGCAAACTA+CGG | - | chr3.2:19594348-19594367 | MS.gene60454:intron | 50.0% | |
| GGGAGATGATTCTGCTGCTA+TGG | + | chr3.2:19594307-19594326 | None:intergenic | 50.0% | |
| GTGAGGGTGGATGTTGAAGT+TGG | - | chr3.2:19595150-19595169 | MS.gene60454:CDS | 50.0% | |
| GTGGAGGAGGAGGAGAAAAT+GGG | + | chr3.2:19594138-19594157 | None:intergenic | 50.0% | |
| !! | TGATATGGTGGTGGTTGTGG+TGG | + | chr3.2:19594184-19594203 | None:intergenic | 50.0% |
| !!! | CCGAATGAGTTTTAAGCGGG+AGG | + | chr3.2:19595266-19595285 | None:intergenic | 50.0% |
| !!! | GAGTTTTAAGCGGGAGGTCT+AGG | + | chr3.2:19595260-19595279 | None:intergenic | 50.0% |
| AGCTGCTGCTGCTGCTGTTA+AGG | + | chr3.2:19594253-19594272 | None:intergenic | 55.0% | |
| CGACGTTCCAGAAGGTTGGA+AGG | - | chr3.2:19594419-19594438 | MS.gene60454:intron | 55.0% | |
| GAGGAGGAGAAAATGGGTGG+TGG | + | chr3.2:19594132-19594151 | None:intergenic | 55.0% | |
| GAGGAGGAGGAGAAAATGGG+TGG | + | chr3.2:19594135-19594154 | None:intergenic | 55.0% | |
| GCAAGTGCAGCAAACTACGG+CGG | - | chr3.2:19594351-19594370 | MS.gene60454:intron | 55.0% | |
| GGTGGAGGAGGAGGAGAAAA+TGG | + | chr3.2:19594139-19594158 | None:intergenic | 55.0% | |
| GGTGGTTGTGGTGGTTGTTG+TGG | + | chr3.2:19594175-19594194 | None:intergenic | 55.0% | |
| GGTTGTGGTGGTTGTTGTGG+TGG | + | chr3.2:19594172-19594191 | None:intergenic | 55.0% | |
| TGCTGCTGCTGCTGTTAAGG+TGG | + | chr3.2:19594250-19594269 | None:intergenic | 55.0% | |
| TGCTGCTGCTGTTAAGGTGG+TGG | + | chr3.2:19594247-19594266 | None:intergenic | 55.0% | |
| TGTGGTGGTTGTTGTGGTGG+TGG | + | chr3.2:19594169-19594188 | None:intergenic | 55.0% | |
| ATTGGACTCAGGCCCACGCT+AGG | - | chr3.2:19595126-19595145 | MS.gene60454:CDS | 60.0% | |
| GCAGCAAACTACGGCGGCTA+CGG | - | chr3.2:19594357-19594376 | MS.gene60454:intron | 60.0% | |
| !! | GGTGGTTGTTGTGGTGGTGG+TGG | + | chr3.2:19594166-19594185 | None:intergenic | 60.0% |
| !! | TGTTGTGGTGGTGGTGGTGC+TGG | + | chr3.2:19594160-19594179 | None:intergenic | 60.0% |
| ATCCACCCTCACGCCTAGCG+TGG | + | chr3.2:19595142-19595161 | None:intergenic | 65.0% | |
| GCAAACTACGGCGGCTACGG+CGG | - | chr3.2:19594360-19594379 | MS.gene60454:intron | 65.0% | |
| TCCACCCTCACGCCTAGCGT+GGG | + | chr3.2:19595141-19595160 | None:intergenic | 65.0% | |
| !! | TGTGGTGGTGGTGGTGCTGG+TGG | + | chr3.2:19594157-19594176 | None:intergenic | 65.0% |
| CAGGCCCACGCTAGGCGTGA+GGG | - | chr3.2:19595134-19595153 | MS.gene60454:CDS | 70.0% | |
| TCAGGCCCACGCTAGGCGTG+AGG | - | chr3.2:19595133-19595152 | MS.gene60454:CDS | 70.0% | |
| !! | GGTGGTGCTGGTGGAGGAGG+AGG | + | chr3.2:19594148-19594167 | None:intergenic | 70.0% |
| !! | GGTGGTGGTGCTGGTGGAGG+AGG | + | chr3.2:19594151-19594170 | None:intergenic | 70.0% |
| !! | GGTGGTGGTGGTGCTGGTGG+AGG | + | chr3.2:19594154-19594173 | None:intergenic | 70.0% |
| GCCCACGCTAGGCGTGAGGG+TGG | - | chr3.2:19595137-19595156 | MS.gene60454:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 19594105 | 19595302 | 19594105 | ID=MS.gene60454 |
| chr3.2 | mRNA | 19594105 | 19595302 | 19594105 | ID=MS.gene60454.t1;Parent=MS.gene60454 |
| chr3.2 | exon | 19594598 | 19595302 | 19594598 | ID=MS.gene60454.t1.exon1;Parent=MS.gene60454.t1 |
| chr3.2 | CDS | 19594598 | 19595302 | 19594598 | ID=cds.MS.gene60454.t1;Parent=MS.gene60454.t1 |
| chr3.2 | exon | 19594105 | 19594338 | 19594105 | ID=MS.gene60454.t1.exon2;Parent=MS.gene60454.t1 |
| chr3.2 | CDS | 19594105 | 19594338 | 19594105 | ID=cds.MS.gene60454.t1;Parent=MS.gene60454.t1 |
| Gene Sequence |
| Protein sequence |
