AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780035939.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780035939.01.T01 MTR_6g004100 90.909 99 7 1 1 97 36 134 2.67e-63 188
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780035939.01.T01 AT5G08410 60.227 88 32 3 10 97 98 182 1.95e-28 101
MsG0780035939.01.T01 AT5G08410 60.227 88 32 3 10 97 118 202 3.01e-28 101
MsG0780035939.01.T01 AT5G23440 62.963 81 27 3 19 99 105 182 8.27e-28 100

Find 20 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAACAGAACGTGACTTTC+TGG 0.222174 7:+352992 MsG0780035939.01.T01:CDS
CGAAGATTGGAGCGCGTGTT+AGG 0.325158 7:+352896 MsG0780035939.01.T01:CDS
GCAACAAATTCAAACTTATA+AGG 0.346872 7:-353045 None:intergenic
GAGATTAACTTCAGGAACTT+TGG 0.356776 7:-352950 None:intergenic
AGATTAACTTCAGGAACTTT+GGG 0.370050 7:-352949 None:intergenic
TGGAGCGCGTGTTAGGGTTA+AGG 0.393600 7:+352903 MsG0780035939.01.T01:CDS
GATTCATCAACTGCGAAGAT+TGG 0.433656 7:+352883 MsG0780035939.01.T01:CDS
CTTCAGGAACTTTGGGAACA+TGG 0.469999 7:-352942 None:intergenic
GAAGATTGGAGCGCGTGTTA+GGG 0.483952 7:+352897 MsG0780035939.01.T01:CDS
TTTGTTGCTGATGATATTCA+AGG 0.490454 7:+353060 MsG0780035939.01.T01:CDS
GTTCCTGAAGTTAATCTCAC+TGG 0.520219 7:+352955 MsG0780035939.01.T01:CDS
GAAGAAGAATGATTCAGATA+AGG 0.523801 7:+352842 None:intergenic
CTACCAGTGAGATTAACTTC+AGG 0.525807 7:-352958 None:intergenic
GGAACATGGTAGACTTTGAG+AGG 0.538879 7:-352928 None:intergenic
GTTAATCTCACTGGTAGAGA+AGG 0.541716 7:+352964 MsG0780035939.01.T01:CDS
ATTCAGATAAGGTGTGATGT+TGG 0.562003 7:+352853 MsG0780035939.01.T01:CDS
AGATGAGCAACAAACTTGAG+AGG 0.590824 7:-353093 None:intergenic
TTAATCTCACTGGTAGAGAA+GGG 0.619049 7:+352965 MsG0780035939.01.T01:CDS
GATGATATTCAAGGTCCTCG+TGG 0.683280 7:+353069 MsG0780035939.01.T01:CDS
ACAAACTTGAGAGGACCACG+AGG 0.762532 7:-353084 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GCAACAAATTCAAACTTATA+AGG - Chr7:353048-353067 None:intergenic 25.0%
AGATTAACTTCAGGAACTTT+GGG - Chr7:352952-352971 None:intergenic 30.0%
TTTGTTGCTGATGATATTCA+AGG + Chr7:353060-353079 MsG0780035939.01.T01:CDS 30.0%
ATTCAGATAAGGTGTGATGT+TGG + Chr7:352853-352872 MsG0780035939.01.T01:CDS 35.0%
GAGATTAACTTCAGGAACTT+TGG - Chr7:352953-352972 None:intergenic 35.0%
TTAATCTCACTGGTAGAGAA+GGG + Chr7:352965-352984 MsG0780035939.01.T01:CDS 35.0%
AGATGAGCAACAAACTTGAG+AGG - Chr7:353096-353115 None:intergenic 40.0%
CTACCAGTGAGATTAACTTC+AGG - Chr7:352961-352980 None:intergenic 40.0%
GATTCATCAACTGCGAAGAT+TGG + Chr7:352883-352902 MsG0780035939.01.T01:CDS 40.0%
GTTAATCTCACTGGTAGAGA+AGG + Chr7:352964-352983 MsG0780035939.01.T01:CDS 40.0%
GTTCCTGAAGTTAATCTCAC+TGG + Chr7:352955-352974 MsG0780035939.01.T01:CDS 40.0%
TCAAACAGAACGTGACTTTC+TGG + Chr7:352992-353011 MsG0780035939.01.T01:CDS 40.0%
GATGATATTCAAGGTCCTCG+TGG + Chr7:353069-353088 MsG0780035939.01.T01:CDS 45.0%
GGAACATGGTAGACTTTGAG+AGG - Chr7:352931-352950 None:intergenic 45.0%
! CTTCAGGAACTTTGGGAACA+TGG - Chr7:352945-352964 None:intergenic 45.0%
ACAAACTTGAGAGGACCACG+AGG - Chr7:353087-353106 None:intergenic 50.0%
GAAGATTGGAGCGCGTGTTA+GGG + Chr7:352897-352916 MsG0780035939.01.T01:CDS 50.0%
CGAAGATTGGAGCGCGTGTT+AGG + Chr7:352896-352915 MsG0780035939.01.T01:CDS 55.0%
TGGAGCGCGTGTTAGGGTTA+AGG + Chr7:352903-352922 MsG0780035939.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 352850 353149 352850 ID=MsG0780035939.01;Name=MsG0780035939.01
Chr7 mRNA 352850 353149 352850 ID=MsG0780035939.01.T01;Parent=MsG0780035939.01;Name=MsG0780035939.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|99
Chr7 exon 352850 353149 352850 ID=MsG0780035939.01.T01:exon:38222;Parent=MsG0780035939.01.T01
Chr7 CDS 352850 353149 352850 ID=MsG0780035939.01.T01:cds;Parent=MsG0780035939.01.T01
Gene Sequence

>MsG0780035939.01.T01

ATGATTCAGATAAGGTGTGATGTTGGAGAAGAAGATTCATCAACTGCGAAGATTGGAGCGCGTGTTAGGGTTAAGGTTCCTCTCAAAGTCTACCATGTTCCCAAAGTTCCTGAAGTTAATCTCACTGGTAGAGAAGGGCGAATCAAACAGAACGTGACTTTCTGGAAAGATAATAAGCGAATCTCTGCTAATCTTCCTTATAAGTTTGAATTTGTTGCTGATGATATTCAAGGTCCTCGTGGTCCTCTCAAGTTTGTTGCTCATCTCAAAGATGATGAATTCAAATTTCTCTCTCAGTAG

Protein sequence

>MsG0780035939.01.T01

MIQIRCDVGEEDSSTAKIGARVRVKVPLKVYHVPKVPEVNLTGREGRIKQNVTFWKDNKRISANLPYKFEFVADDIQGPRGPLKFVAHLKDDEFKFLSQ*