AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047723.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047723.01.T01 MTR_4g133770 96.859 191 6 0 1 191 1 191 3.47e-133 371
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047723.01.T01 AT4G16410 62.609 115 41 2 77 190 71 184 2.02e-43 143

Find 39 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGAGGGGTTGGAGGAATT+TGG 0.164087 8:-90033523 None:intergenic
ATTCTCATCATTGCTATTCT+TGG 0.233178 8:+90033961 MsG0880047723.01.T01:CDS
CACTTCTGGAAGAAGAGTTT+TGG 0.247345 8:+90033833 MsG0880047723.01.T01:CDS
GAGCAAAAGGCATTGGCTTT+TGG 0.251573 8:+90033799 MsG0880047723.01.T01:CDS
ATGATGAGAATTGCAGTAAT+TGG 0.254419 8:-90033949 None:intergenic
ATGGGTTTGATAAATACTTT+TGG 0.309917 8:-90033559 None:intergenic
TGGACCTGAAGGACCACTTC+TGG 0.347564 8:+90033819 MsG0880047723.01.T01:CDS
TCTTCCAGAAGTGGTCCTTC+AGG 0.352779 8:-90033823 None:intergenic
TTTAATGCCTAATACTTTGA+TGG 0.358229 8:-90033578 None:intergenic
AATTCAAAGATTGTGATAAT+AGG 0.375780 8:+90033733 MsG0880047723.01.T01:CDS
AGAAATGGAAGTTTCATTGA+TGG 0.380684 8:-90033700 None:intergenic
ATCAAACCCATCAAAGTATT+AGG 0.381123 8:+90033571 MsG0880047723.01.T01:CDS
CCACAGTTGGTGTGTGTTTG+AGG 0.388523 8:-90033660 None:intergenic
GGTGTGTGTTTGAGGAAAAT+TGG 0.393174 8:-90033652 None:intergenic
TTCTCAAGTCCTTACTGCTA+TGG 0.416025 8:+90034002 MsG0880047723.01.T01:CDS
CTCATCATTGCTATTCTTGG+AGG 0.417146 8:+90033964 MsG0880047723.01.T01:CDS
ATCATTGCTATTCTTGGAGG+TGG 0.422996 8:+90033967 MsG0880047723.01.T01:CDS
CTTTGTGCATCTTCCACAGT+TGG 0.439217 8:-90033673 None:intergenic
GAAAAGAGAACAAGAGGGGT+TGG 0.443409 8:-90033533 None:intergenic
GAATGGAATGGATGAGCAAA+AGG 0.459747 8:+90033786 MsG0880047723.01.T01:CDS
AAGAGAACAAGAGGGGTTGG+AGG 0.462285 8:-90033530 None:intergenic
TTAATGCCTAATACTTTGAT+GGG 0.463239 8:-90033577 None:intergenic
ATAATAGGAGCAGTTTCTCT+TGG 0.477281 8:+90033748 MsG0880047723.01.T01:CDS
TGGGACAACGTGAGAAGGTA+TGG 0.479294 8:+90033853 MsG0880047723.01.T01:CDS
AACTGTGGAAGATGCACAAA+GGG 0.491879 8:+90033675 MsG0880047723.01.T01:CDS
GGTTGGAGGAATTTGGAAGC+TGG 0.507532 8:-90033516 None:intergenic
TTCTTGAGAAGCTCAAATAT+AGG 0.508858 8:-90033925 None:intergenic
AGGTGCAATATACACAATCT+TGG 0.535873 8:+90033900 MsG0880047723.01.T01:CDS
CAAGTCCTTACTGCTATGGT+TGG 0.540150 8:+90034006 MsG0880047723.01.T01:CDS
CAAAACTCTTCTTCCAGAAG+TGG 0.542465 8:-90033832 None:intergenic
TTTGTTGGTTCTGAATGGAA+TGG 0.544407 8:+90033774 MsG0880047723.01.T01:CDS
TATGCACTAACTGTTAGTAC+AGG 0.569987 8:+90033880 MsG0880047723.01.T01:CDS
AATGGATGAGCAAAAGGCAT+TGG 0.578741 8:+90033792 MsG0880047723.01.T01:CDS
AAACGCCAACCATAGCAGTA+AGG 0.591938 8:-90034011 None:intergenic
TGGAGTGAAAAGAGAACAAG+AGG 0.597398 8:-90033539 None:intergenic
CCTCAAACACACACCAACTG+TGG 0.606028 8:+90033660 MsG0880047723.01.T01:CDS
GAGTGAAAAGAGAACAAGAG+GGG 0.620816 8:-90033537 None:intergenic
CAACTGTGGAAGATGCACAA+AGG 0.630847 8:+90033674 MsG0880047723.01.T01:CDS
GGAGTGAAAAGAGAACAAGA+GGG 0.645371 8:-90033538 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCAAAGATTGTGATAAT+AGG + Chr8:90033733-90033752 MsG0880047723.01.T01:CDS 20.0%
!!! AGTTTTATTGCATATTTTGA+AGG - Chr8:90033619-90033638 None:intergenic 20.0%
! TTAATGCCTAATACTTTGAT+GGG - Chr8:90033580-90033599 None:intergenic 25.0%
! TTTAATGCCTAATACTTTGA+TGG - Chr8:90033581-90033600 None:intergenic 25.0%
!! ATGGGTTTGATAAATACTTT+TGG - Chr8:90033562-90033581 None:intergenic 25.0%
!!! GAATTCTCATTTTCAAGAAA+TGG - Chr8:90033718-90033737 None:intergenic 25.0%
!!! TTATTGCATATTTTGAAGGT+GGG - Chr8:90033615-90033634 None:intergenic 25.0%
!!! TTTATTGCATATTTTGAAGG+TGG - Chr8:90033616-90033635 None:intergenic 25.0%
AGAAATGGAAGTTTCATTGA+TGG - Chr8:90033703-90033722 None:intergenic 30.0%
ATGATGAGAATTGCAGTAAT+TGG - Chr8:90033952-90033971 None:intergenic 30.0%
TTCTTGAGAAGCTCAAATAT+AGG - Chr8:90033928-90033947 None:intergenic 30.0%
! AGTTTCTCTTGGAATTTTGT+TGG + Chr8:90033759-90033778 MsG0880047723.01.T01:CDS 30.0%
! ATCAAACCCATCAAAGTATT+AGG + Chr8:90033571-90033590 MsG0880047723.01.T01:CDS 30.0%
! ATTCTCATCATTGCTATTCT+TGG + Chr8:90033961-90033980 MsG0880047723.01.T01:CDS 30.0%
AGGTGCAATATACACAATCT+TGG + Chr8:90033900-90033919 MsG0880047723.01.T01:CDS 35.0%
ATAATAGGAGCAGTTTCTCT+TGG + Chr8:90033748-90033767 MsG0880047723.01.T01:CDS 35.0%
TATGCACTAACTGTTAGTAC+AGG + Chr8:90033880-90033899 MsG0880047723.01.T01:CDS 35.0%
!! ACTTCTGGAAGAAGAGTTTT+GGG + Chr8:90033834-90033853 MsG0880047723.01.T01:CDS 35.0%
!! TTTGTTGGTTCTGAATGGAA+TGG + Chr8:90033774-90033793 MsG0880047723.01.T01:CDS 35.0%
!!! GGAATTTTGTTGGTTCTGAA+TGG + Chr8:90033769-90033788 MsG0880047723.01.T01:CDS 35.0%
AACTGTGGAAGATGCACAAA+GGG + Chr8:90033675-90033694 MsG0880047723.01.T01:CDS 40.0%
CAAAACTCTTCTTCCAGAAG+TGG - Chr8:90033835-90033854 None:intergenic 40.0%
GAATGGAATGGATGAGCAAA+AGG + Chr8:90033786-90033805 MsG0880047723.01.T01:CDS 40.0%
GAGTGAAAAGAGAACAAGAG+GGG - Chr8:90033540-90033559 None:intergenic 40.0%
GGAGTGAAAAGAGAACAAGA+GGG - Chr8:90033541-90033560 None:intergenic 40.0%
GGTGTGTGTTTGAGGAAAAT+TGG - Chr8:90033655-90033674 None:intergenic 40.0%
TGGAGTGAAAAGAGAACAAG+AGG - Chr8:90033542-90033561 None:intergenic 40.0%
TTCTCAAGTCCTTACTGCTA+TGG + Chr8:90034002-90034021 MsG0880047723.01.T01:CDS 40.0%
! AATGGATGAGCAAAAGGCAT+TGG + Chr8:90033792-90033811 MsG0880047723.01.T01:CDS 40.0%
! ATCATTGCTATTCTTGGAGG+TGG + Chr8:90033967-90033986 MsG0880047723.01.T01:CDS 40.0%
! CTCATCATTGCTATTCTTGG+AGG + Chr8:90033964-90033983 MsG0880047723.01.T01:CDS 40.0%
!! CACTTCTGGAAGAAGAGTTT+TGG + Chr8:90033833-90033852 MsG0880047723.01.T01:CDS 40.0%
AAACGCCAACCATAGCAGTA+AGG - Chr8:90034014-90034033 None:intergenic 45.0%
CAACTGTGGAAGATGCACAA+AGG + Chr8:90033674-90033693 MsG0880047723.01.T01:CDS 45.0%
CAAGTCCTTACTGCTATGGT+TGG + Chr8:90034006-90034025 MsG0880047723.01.T01:CDS 45.0%
CTTTGTGCATCTTCCACAGT+TGG - Chr8:90033676-90033695 None:intergenic 45.0%
GAAAAGAGAACAAGAGGGGT+TGG - Chr8:90033536-90033555 None:intergenic 45.0%
!!! AGTTTTGGGACAACGTGAGA+AGG + Chr8:90033848-90033867 MsG0880047723.01.T01:CDS 45.0%
!!! GAGCAAAAGGCATTGGCTTT+TGG + Chr8:90033799-90033818 MsG0880047723.01.T01:CDS 45.0%
AAGAGAACAAGAGGGGTTGG+AGG - Chr8:90033533-90033552 None:intergenic 50.0%
CAAGAGGGGTTGGAGGAATT+TGG - Chr8:90033526-90033545 None:intergenic 50.0%
CCTCAAACACACACCAACTG+TGG + Chr8:90033660-90033679 MsG0880047723.01.T01:CDS 50.0%
GGTTGGAGGAATTTGGAAGC+TGG - Chr8:90033519-90033538 None:intergenic 50.0%
TCTTCCAGAAGTGGTCCTTC+AGG - Chr8:90033826-90033845 None:intergenic 50.0%
TGGGACAACGTGAGAAGGTA+TGG + Chr8:90033853-90033872 MsG0880047723.01.T01:CDS 50.0%
! CCACAGTTGGTGTGTGTTTG+AGG - Chr8:90033663-90033682 None:intergenic 50.0%
!!! GCATTGGCTTTTGGACCTGA+AGG + Chr8:90033808-90033827 MsG0880047723.01.T01:CDS 50.0%
TGGACCTGAAGGACCACTTC+TGG + Chr8:90033819-90033838 MsG0880047723.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 90033487 90034062 90033487 ID=MsG0880047723.01;Name=MsG0880047723.01
Chr8 mRNA 90033487 90034062 90033487 ID=MsG0880047723.01.T01;Parent=MsG0880047723.01;Name=MsG0880047723.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|191
Chr8 exon 90033487 90034062 90033487 ID=MsG0880047723.01.T01:exon:2587;Parent=MsG0880047723.01.T01
Chr8 CDS 90033487 90034062 90033487 ID=MsG0880047723.01.T01:cds;Parent=MsG0880047723.01.T01
Gene Sequence

>MsG0880047723.01.T01

ATGAAAAATCTTAGCTTGAAATTAAAGCTCCAGCTTCCAAATTCCTCCAACCCCTCTTGTTCTCTTTTCACTCCAAAAGTATTTATCAAACCCATCAAAGTATTAGGCATTAAAAACTTCAAAAACCCACCTTCAAAATATGCAATAAAACTTAGAGAAGCTTCTCCAATTTTCCTCAAACACACACCAACTGTGGAAGATGCACAAAGGGCACCATCAATGAAACTTCCATTTCTTGAAAATGAGAATTCAAAGATTGTGATAATAGGAGCAGTTTCTCTTGGAATTTTGTTGGTTCTGAATGGAATGGATGAGCAAAAGGCATTGGCTTTTGGACCTGAAGGACCACTTCTGGAAGAAGAGTTTTGGGACAACGTGAGAAGGTATGGTTTGTATGCACTAACTGTTAGTACAGGTGCAATATACACAATCTTGGTGCCTATATTTGAGCTTCTCAAGAATCCAATTACTGCAATTCTCATCATTGCTATTCTTGGAGGTGGTTTCTACATTGTTTCTCAAGTCCTTACTGCTATGGTTGGCGTTTCTGATTTTAGTTATGATTATGCAAACTAG

Protein sequence

>MsG0880047723.01.T01

MKNLSLKLKLQLPNSSNPSCSLFTPKVFIKPIKVLGIKNFKNPPSKYAIKLREASPIFLKHTPTVEDAQRAPSMKLPFLENENSKIVIIGAVSLGILLVLNGMDEQKALAFGPEGPLLEEEFWDNVRRYGLYALTVSTGAIYTILVPIFELLKNPITAILIIAILGGGFYIVSQVLTAMVGVSDFSYDYAN*