AlfalfaGEDB | Msa0086180

M. sativa cultivar zhongmu-4 / Msa0086180

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0086180	XP_003592080.1	94.565	184	10	0	12	195	1	184	4.63e-124	358

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0086180	G7IBX7	94.565	184	10	0	12	195	1	184	2.21e-124	358

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006290	Msa0086180	0.800659	1.299103e-48	-8.615850e-47
Msa0011960	Msa0086180	0.830760	2.448944e-55	-8.615850e-47
Msa0013050	Msa0086180	-0.801213	1.001263e-48	-8.615850e-47
Msa0025730	Msa0086180	0.817582	3.060101e-52	-8.615850e-47
Msa0027770	Msa0086180	0.801420	9.079820e-49	-8.615850e-47
Msa0032580	Msa0086180	0.823355	1.448377e-53	-8.615850e-47
Msa0033110	Msa0086180	0.820704	5.959488e-53	-8.615850e-47
Msa0033260	Msa0086180	0.812829	3.481850e-51	-8.615850e-47
Msa0043660	Msa0086180	0.867415	1.391620e-65	-8.615850e-47
Msa0047160	Msa0086180	0.839685	1.367137e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0086180	MtrunA17_Chr1g0201701	94.565	184	10	0	12	195	1	184	4.26e-128	358

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0086180	AT5G09270.1	55.789	95	37	2	101	195	19	108	1.88e-30	108
Msa0086180	AT5G09270.2	55.789	95	37	2	101	195	19	108	1.88e-30	108

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Find 53 sgRNAs with CRISPR-Local

Find 392 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAAGCTTTCTATATCTAT+AGG	0.413891	1_2:+66793325	None:intergenic
AAGTTCTGCAGCACCTATCC+TGG	0.422828	1_2:-66793504	Msa0086180:CDS
ACAACGTGAGATGGTTGAAA+AGG	0.483137	1_2:-66793267	Msa0086180:CDS
ACCTATCCTGGAAACTGGTG+AGG	0.641675	1_2:-66793492	Msa0086180:CDS
ACTGGCGAGGATCAAGTAGT+CGG	0.654403	1_2:-66793650	Msa0086180:CDS
ACTGGTGAGGACCAAGTAGT+CGG	0.664340	1_2:-66793479	Msa0086180:CDS
ACTTATCCAGAAAACTGGCG+AGG	0.696112	1_2:-66793663	Msa0086180:CDS
AGAAACTTATCCTTATGTAT+GGG	0.501079	1_2:-66793390	Msa0086180:CDS
AGCTTCTTAGGACAGAGGAC+AGG	0.494425	1_2:-66793310	Msa0086180:CDS
AGGATAGGTGCTGCAGAACT+TGG	0.446859	1_2:+66793506	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGTAACACATATACCTCTT+AGG	-	chr1_2:66793250-66793269	Msa0086180:CDS	30.0%
ACTATGAAAGAACATACACT+TGG	+	chr1_2:66793318-66793337	None:intergenic	30.0%
ATATTGCAGAAGTGATTGAT+AGG	-	chr1_2:66793581-66793600	Msa0086180:CDS	30.0%
GAAATCTATGAGAAAAAGTG+TGG	-	chr1_2:66794083-66794102	Msa0086180:intron	30.0%
AAAACGAATGCAATCAACAA+GGG	+	chr1_2:66794259-66794278	None:intergenic	30.0%
AAAAACGAATGCAATCAACA+AGG	+	chr1_2:66794260-66794279	None:intergenic	30.0%
AACATACTTCTATGTTCAGT+GGG	-	chr1_2:66794291-66794310	Msa0086180:intron	30.0%
TAATTCAGGGACTAATTTGA+TGG	+	chr1_2:66794773-66794792	None:intergenic	30.0%
AACTTGATAAACTAGAGGAA+AGG	-	chr1_2:66795208-66795227	Msa0086180:intron	30.0%
TTGTCAAACATCCATCAAAA+AGG	+	chr1_2:66795575-66795594	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	66793181	66799195	66793181	ID=Msa0086180;Name=Msa0086180
chr1_2	mRNA	66793181	66799195	66793181	ID=Msa0086180-mRNA-1;Parent=Msa0086180;Name=Msa0086180-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0\|0\|0\|0.66\|0\|0.33\|3\|0\|195
chr1_2	exon	66799192	66799195	66799192	ID=Msa0086180-mRNA-1:exon:22585;Parent=Msa0086180-mRNA-1
chr1_2	exon	66793796	66793803	66793796	ID=Msa0086180-mRNA-1:exon:22584;Parent=Msa0086180-mRNA-1
chr1_2	exon	66793181	66793756	66793181	ID=Msa0086180-mRNA-1:exon:22583;Parent=Msa0086180-mRNA-1
chr1_2	CDS	66799192	66799195	66799192	ID=Msa0086180-mRNA-1:cds;Parent=Msa0086180-mRNA-1
chr1_2	CDS	66793796	66793803	66793796	ID=Msa0086180-mRNA-1:cds;Parent=Msa0086180-mRNA-1
chr1_2	CDS	66793181	66793756	66793181	ID=Msa0086180-mRNA-1:cds;Parent=Msa0086180-mRNA-1

Gene Sequence

>Msa0086180

ATGATGCTGAAACTGGAACACTTAGCCAAGAAAATGGCAAATCAACCAAATAATGAAAAGCAGCAAGCACCAAGTTCTGAAGCACTTATCCAGAAAACTGGCGAGGATCAAGTAGTCGGTGGCTCTTCTGCTACGGGCTTTATGGATTCATTGAAAAATCGGTATCAAAAAATCATAGATAGCTGGAACACTCCAGCCAAGGACATGGCAAATCAACCAAATAATGAAAAGCAACAAGCACCAAGTTCTGCAGCACCTATCCTGGAAACTGGTGAGGACCAAGTAGTCGGTGCCCCTCCTGCTGCAGGCTTTATGGATTCATTGGAAAATCGGTATAAAAAAATCAAAGAGCATGCAGAAACTTATCCTTATGTATGGGGTTCCTATATTCTTGTATATGGCGGATTTGGTCTTTGGTTTGCCTATAGATATAGAAAGCTTCTTAGGACAGAGGACAGGGTACGGGCACTTCAAGCAAGACAACGTGAGATGGTTGAAAAGGATAAGATTGTTGAAGCTGAAAATTCTAGTAATTCTGCCAAAGTGGTTGAAAAGGGTTCCACATCTTCTGAGATTCCTTCCAAGTAG

Protein sequence

>Msa0086180

MMLKLEHLAKKMANQPNNEKQQAPSSEALIQKTGEDQVVGGSSATGFMDSLKNRYQKIIDSWNTPAKDMANQPNNEKQQAPSSAAPILETGEDQVVGAPPAAGFMDSLENRYKKIKEHAETYPYVWGSYILVYGGFGLWFAYRYRKLLRTEDRVRALQARQREMVEKDKIVEAENSSNSAKVVEKGSTSSEIPSK*