AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0214710


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0214710 MtrunA17_Chr2g0315721 98.276 58 1 0 1 58 1 58 3.01e-37 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0214710 AT1G10865.2 74.576 59 15 0 1 59 1 59 2.32e-26 90.9
Msa0214710 AT1G10865.1 74.576 59 15 0 1 59 1 59 3.78e-26 90.9

Find 14 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTGAGTGTGTTGGTCTT+AGG 0.172998 2_1:+59296135 Msa0214710:CDS
AGCTGAGTGTGTTGGTCTTA+GGG 0.357346 2_1:+59296136 Msa0214710:CDS
GGCTAATCCTTTGCAAGACT+TGG 0.367609 2_1:-59295927 None:intergenic
ACCTATTTCAATTGCAAAAG+AGG 0.400990 2_1:+59296161 Msa0214710:CDS
CAGTGAATCTGATTGTGTTA+AGG 0.440858 2_1:+59295970 Msa0214710:CDS
TCAATTTCAGCTGAGTGTGT+TGG 0.497821 2_1:+59296128 Msa0214710:CDS
ACACACTCAGCTGAAATTGA+AGG 0.502802 2_1:-59296125 None:intergenic
TTCACGTTTAAGAGAGGTGA+AGG 0.516785 2_1:+59295888 None:intergenic
TTTCAATTGCAAAAGAGGAC+AGG 0.525074 2_1:+59296166 Msa0214710:CDS
AGATCATACAAGGAGTGTGC+TGG 0.532397 2_1:+59296092 Msa0214710:CDS
GAGACACTTAACTAATTCCA+TGG 0.532404 2_1:-59295948 None:intergenic
GTCTTGCAAAGGATTAGCCA+TGG 0.600408 2_1:+59295931 Msa0214710:CDS
GGTTGATAATAGATCATACA+AGG 0.601750 2_1:+59296082 Msa0214710:intron
ACAATGTCCAAGTCTTGCAA+AGG 0.613550 2_1:+59295920 Msa0214710:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATGTTTATGATTATGAAT+AGG + chr2_1:59296061-59296080 Msa0214710:intron 15.0%
!!! TGATTTTTGTGATGTTAAAA+TGG + chr2_1:59296037-59296056 Msa0214710:intron 20.0%
!!! GATTTTTGTGATGTTAAAAT+GGG + chr2_1:59296038-59296057 Msa0214710:intron 20.0%
!! GGTTGATAATAGATCATACA+AGG + chr2_1:59296082-59296101 Msa0214710:intron 30.0%
ACCTATTTCAATTGCAAAAG+AGG + chr2_1:59296161-59296180 Msa0214710:CDS 30.0%
! TCCTCTTTTGCAATTGAAAT+AGG - chr2_1:59296165-59296184 None:intergenic 30.0%
GAGACACTTAACTAATTCCA+TGG - chr2_1:59295951-59295970 None:intergenic 35.0%
CAGTGAATCTGATTGTGTTA+AGG + chr2_1:59295970-59295989 Msa0214710:CDS 35.0%
TTTCAATTGCAAAAGAGGAC+AGG + chr2_1:59296166-59296185 Msa0214710:CDS 35.0%
ACAATGTCCAAGTCTTGCAA+AGG + chr2_1:59295920-59295939 Msa0214710:exon 40.0%
ACACACTCAGCTGAAATTGA+AGG - chr2_1:59296128-59296147 None:intergenic 40.0%
TCAATTTCAGCTGAGTGTGT+TGG + chr2_1:59296128-59296147 Msa0214710:CDS 40.0%
GGCTAATCCTTTGCAAGACT+TGG - chr2_1:59295930-59295949 None:intergenic 45.0%
!! GTCTTGCAAAGGATTAGCCA+TGG + chr2_1:59295931-59295950 Msa0214710:CDS 45.0%
! AGATCATACAAGGAGTGTGC+TGG + chr2_1:59296092-59296111 Msa0214710:CDS 45.0%
!! AGCTGAGTGTGTTGGTCTTA+GGG + chr2_1:59296136-59296155 Msa0214710:CDS 45.0%
!! CAGCTGAGTGTGTTGGTCTT+AGG + chr2_1:59296135-59296154 Msa0214710:CDS 50.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 59295894 59296190 59295894 ID=Msa0214710;Name=Msa0214710
chr2_1 mRNA 59295894 59296190 59295894 ID=Msa0214710-mRNA-1;Parent=Msa0214710;Name=Msa0214710-mRNA-1;_AED=0.04;_eAED=0.04;_QI=29|1|0.5|1|1|1|2|0|59
chr2_1 exon 59295894 59295991 59295894 ID=Msa0214710-mRNA-1:exon:16319;Parent=Msa0214710-mRNA-1
chr2_1 exon 59296083 59296190 59296083 ID=Msa0214710-mRNA-1:exon:16320;Parent=Msa0214710-mRNA-1
chr2_1 five_prime_UTR 59295894 59295922 59295894 ID=Msa0214710-mRNA-1:five_prime_utr;Parent=Msa0214710-mRNA-1
chr2_1 CDS 59295923 59295991 59295923 ID=Msa0214710-mRNA-1:cds;Parent=Msa0214710-mRNA-1
chr2_1 CDS 59296083 59296190 59296083 ID=Msa0214710-mRNA-1:cds;Parent=Msa0214710-mRNA-1
Gene Sequence

>Msa0214710

ATGTCCAAGTCTTGCAAAGGATTAGCCATGGAATTAGTTAAGTGTCTCAGTGAATCTGATTGTGTTAAGGTTGATAATAGATCATACAAGGAGTGTGCTGGAGAGAAGACTCCTTCAATTTCAGCTGAGTGTGTTGGTCTTAGGGAAACCTATTTCAATTGCAAAAGAGGACAGGCA

Protein sequence

>Msa0214710

MSKSCKGLAMELVKCLSESDCVKVDNRSYKECAGEKTPSISAECVGLRETYFNCKRGQA