AlfalfaGEDB | Msa0834130

M. sativa cultivar zhongmu-4 / Msa0834130

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0834130	XP_013453441.1	92.881	295	18	1	1	292	1	295	7.18e-171	484

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0834130	A0A072UNQ3	92.881	295	18	1	1	292	1	295	3.43e-171	484

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0008850	Msa0834130	0.837802	4.193062e-57	-8.615850e-47
Msa0011720	Msa0834130	0.806132	9.544444e-50	-8.615850e-47
Msa0017120	Msa0834130	0.800509	1.394278e-48	-8.615850e-47
Msa0020630	Msa0834130	0.804502	2.094864e-49	-8.615850e-47
Msa0027540	Msa0834130	0.817765	2.782523e-52	-8.615850e-47
Msa0030000	Msa0834130	0.812255	4.649282e-51	-8.615850e-47
Msa0044710	Msa0834130	0.801823	7.506915e-49	-8.615850e-47
Msa0075310	Msa0834130	0.820909	5.346810e-53	-8.615850e-47
Msa0084680	Msa0834130	0.807624	4.616268e-50	-8.615850e-47
Msa0091770	Msa0834130	0.806735	7.120602e-50	-8.615850e-47
Msa0105340	Msa0834130	0.808093	3.668943e-50	-8.615850e-47
Msa0605620	Msa0834130	0.805705	1.173065e-49	-8.615850e-47
Msa0606060	Msa0834130	0.822116	2.813127e-53	-8.615850e-47
Msa0617370	Msa0834130	0.801092	1.059935e-48	-8.615850e-47
Msa0619330	Msa0834130	0.826931	2.068459e-54	-8.615850e-47
Msa0621890	Msa0834130	0.818551	1.847747e-52	-8.615850e-47
Msa0624730	Msa0834130	0.840971	6.305416e-58	-8.615850e-47
Msa0631840	Msa0834130	0.810458	1.141331e-50	-8.615850e-47
Msa0647000	Msa0834130	0.827122	1.861934e-54	-8.615850e-47
Msa0655660	Msa0834130	-0.817402	3.358949e-52	-8.615850e-47
Msa0674220	Msa0834130	0.843967	1.011552e-58	-8.615850e-47
Msa0674300	Msa0834130	0.818860	1.572597e-52	-8.615850e-47
Msa0681890	Msa0834130	0.818271	2.137969e-52	-8.615850e-47
Msa0686610	Msa0834130	0.830663	2.586762e-55	-8.615850e-47
Msa0707780	Msa0834130	0.805725	1.161798e-49	-8.615850e-47
Msa0714600	Msa0834130	0.828564	8.379537e-55	-8.615850e-47
Msa0723140	Msa0834130	0.802188	6.315508e-49	-8.615850e-47
Msa0724050	Msa0834130	0.834311	3.226082e-56	-8.615850e-47
Msa0726610	Msa0834130	0.827391	1.605582e-54	-8.615850e-47
Msa0730180	Msa0834130	0.801787	7.637049e-49	-8.615850e-47
Msa0733000	Msa0834130	0.818842	1.587001e-52	-8.615850e-47
Msa0739570	Msa0834130	0.811964	5.380247e-51	-8.615850e-47
Msa0750380	Msa0834130	0.802474	5.514862e-49	-8.615850e-47
Msa0754860	Msa0834130	0.931231	5.122447e-94	-8.615850e-47
Msa0763880	Msa0834130	0.824212	9.120320e-54	-8.615850e-47
Msa0763910	Msa0834130	0.809614	1.734508e-50	-8.615850e-47
Msa0772150	Msa0834130	0.803309	3.706623e-49	-8.615850e-47
Msa0772210	Msa0834130	0.810820	9.529573e-51	-8.615850e-47
Msa0791070	Msa0834130	0.808639	2.805513e-50	-8.615850e-47
Msa0796590	Msa0834130	0.979836	7.509268e-149	-8.615850e-47
Msa0796810	Msa0834130	0.904236	1.514553e-79	-8.615850e-47
Msa0810020	Msa0834130	0.801132	1.040006e-48	-8.615850e-47
Msa0811510	Msa0834130	0.810236	1.274077e-50	-8.615850e-47
Msa0820670	Msa0834130	0.805223	1.480486e-49	-8.615850e-47
Msa0828570	Msa0834130	0.802905	4.494203e-49	-8.615850e-47
Msa0830720	Msa0834130	0.801083	1.064234e-48	-8.615850e-47
Msa0833940	Msa0834130	0.949009	3.021973e-107	-8.615850e-47
Msa0834130	Msa0837990	0.816100	6.581910e-52	-8.615850e-47
Msa0834130	Msa0847200	0.812930	3.308932e-51	-8.615850e-47
Msa0834130	Msa0851740	0.816232	6.148990e-52	-8.615850e-47
Msa0834130	Msa0860270	0.815883	7.356392e-52	-8.615850e-47
Msa0834130	Msa0890250	0.800405	1.463774e-48	-8.615850e-47
Msa0834130	Msa0903730	0.801095	1.058411e-48	-8.615850e-47
Msa0834130	Msa0925160	0.806611	7.564028e-50	-8.615850e-47
Msa0834130	Msa0952950	0.806413	8.327977e-50	-8.615850e-47
Msa0834130	Msa0952990	0.813448	2.547186e-51	-8.615850e-47
Msa0834130	Msa0971860	0.810268	1.254489e-50	-8.615850e-47
Msa0834130	Msa0973230	0.815863	7.434924e-52	-8.615850e-47
Msa0834130	Msa0980440	0.800283	1.549930e-48	-8.615850e-47
Msa0834130	Msa0990520	0.818374	2.027017e-52	-8.615850e-47
Msa0834130	Msa1038320	0.857395	1.680902e-62	-8.615850e-47
Msa0834130	Msa1044040	0.801415	9.103764e-49	-8.615850e-47
Msa0834130	Msa1072350	0.813069	3.084848e-51	-8.615850e-47
Msa0834130	Msa1089530	0.821889	3.175963e-53	-8.615850e-47
Msa0834130	Msa1118140	0.816463	5.459963e-52	-8.615850e-47
Msa0834130	Msa1140010	0.809183	2.146286e-50	-8.615850e-47
Msa0834130	Msa1166180	0.800289	1.545549e-48	-8.615850e-47
Msa0834130	Msa1172590	0.810875	9.272232e-51	-8.615850e-47
Msa0834130	Msa1204120	0.812263	4.628386e-51	-8.615850e-47
Msa0834130	Msa1210770	0.807051	6.105081e-50	-8.615850e-47
Msa0834130	Msa1220230	0.801523	8.651695e-49	-8.615850e-47
Msa0834130	Msa1262310	0.807264	5.503232e-50	-8.615850e-47
Msa0834130	Msa1265960	0.819794	9.632439e-53	-8.615850e-47
Msa0834130	Msa1303780	0.809514	1.822331e-50	-8.615850e-47
Msa0834130	Msa1335970	-0.805178	1.513148e-49	-8.615850e-47
Msa0834130	Msa1341930	0.802912	4.479353e-49	-8.615850e-47
Msa0834130	Msa1343820	0.808765	2.637073e-50	-8.615850e-47
Msa0834130	Msa1347620	0.813770	2.162845e-51	-8.615850e-47
Msa0834130	Msa1352290	0.810085	1.373494e-50	-8.615850e-47
Msa0834130	Msa1355260	0.823418	1.400291e-53	-8.615850e-47
Msa0834130	Msa1381130	0.809902	1.503965e-50	-8.615850e-47
Msa0834130	Msa1388040	0.987131	3.642106e-169	-8.615850e-47
Msa0834130	Msa1395570	0.816103	6.570302e-52	-8.615850e-47
Msa0834130	Msa1405180	0.806654	7.405155e-50	-8.615850e-47
Msa0834130	Msa1418200	0.832414	9.583603e-56	-8.615850e-47
Msa0834130	Msa1428420	0.827690	1.361052e-54	-8.615850e-47
Msa0834130	Msa1431000	0.822601	2.170993e-53	-8.615850e-47
Msa0834130	Msa1435470	0.805676	1.190117e-49	-8.615850e-47
Msa0834130	Msa1447560	0.847425	1.165650e-59	-8.615850e-47
Msa0834130	Msa1456810	0.855295	6.945838e-62	-8.615850e-47
Msa0834130	Msa1458920	0.810179	1.310620e-50	-8.615850e-47
Msa0834130	Msa1464560	0.850247	1.920673e-60	-8.615850e-47
Msa0125230	Msa0834130	0.812733	3.654626e-51	-8.615850e-47
Msa0141670	Msa0834130	0.801877	7.317430e-49	-8.615850e-47
Msa0146020	Msa0834130	0.803655	3.142502e-49	-8.615850e-47
Msa0159750	Msa0834130	0.807021	6.194338e-50	-8.615850e-47
Msa0164360	Msa0834130	0.850073	2.149545e-60	-8.615850e-47
Msa0168100	Msa0834130	0.814397	1.572817e-51	-8.615850e-47
Msa0182930	Msa0834130	0.801216	9.997547e-49	-8.615850e-47
Msa0185390	Msa0834130	0.803124	4.048783e-49	-8.615850e-47
Msa0185480	Msa0834130	0.818796	1.625992e-52	-8.615850e-47
Msa0188630	Msa0834130	0.804993	1.654310e-49	-8.615850e-47
Msa0194570	Msa0834130	0.801594	8.365960e-49	-8.615850e-47
Msa0203640	Msa0834130	0.803704	3.070769e-49	-8.615850e-47
Msa0207000	Msa0834130	0.824789	6.671846e-54	-8.615850e-47
Msa0208520	Msa0834130	0.814373	1.592469e-51	-8.615850e-47
Msa0222070	Msa0834130	0.813876	2.049541e-51	-8.615850e-47
Msa0222120	Msa0834130	0.816347	5.794958e-52	-8.615850e-47
Msa0227500	Msa0834130	0.808598	2.862681e-50	-8.615850e-47
Msa0371610	Msa0834130	0.809335	1.990484e-50	-8.615850e-47
Msa0413740	Msa0834130	0.807930	3.973342e-50	-8.615850e-47
Msa0418000	Msa0834130	0.807241	5.565257e-50	-8.615850e-47
Msa0423360	Msa0834130	-0.806693	7.266248e-50	-8.615850e-47
Msa0427050	Msa0834130	0.805560	1.258656e-49	-8.615850e-47
Msa0458790	Msa0834130	0.805553	1.262748e-49	-8.615850e-47
Msa0459120	Msa0834130	0.805320	1.413062e-49	-8.615850e-47
Msa0480320	Msa0834130	0.804571	2.026426e-49	-8.615850e-47
Msa0482680	Msa0834130	0.822376	2.448284e-53	-8.615850e-47
Msa0240780	Msa0834130	0.817341	3.467274e-52	-8.615850e-47
Msa0252180	Msa0834130	0.847441	1.154113e-59	-8.615850e-47
Msa0257130	Msa0834130	0.804357	2.245283e-49	-8.615850e-47
Msa0263620	Msa0834130	0.811023	8.615181e-51	-8.615850e-47
Msa0278620	Msa0834130	0.830416	2.973506e-55	-8.615850e-47
Msa0282170	Msa0834130	0.800200	1.611537e-48	-8.615850e-47
Msa0288240	Msa0834130	0.822309	2.537565e-53	-8.615850e-47
Msa0288330	Msa0834130	0.817284	3.571503e-52	-8.615850e-47
Msa0296760	Msa0834130	0.810373	1.190754e-50	-8.615850e-47
Msa0298940	Msa0834130	0.814418	1.556061e-51	-8.615850e-47
Msa0298970	Msa0834130	0.812814	3.507672e-51	-8.615850e-47
Msa0301180	Msa0834130	0.827891	1.217847e-54	-8.615850e-47
Msa0306130	Msa0834130	0.817359	3.435837e-52	-8.615850e-47
Msa0318000	Msa0834130	0.832584	8.698577e-56	-8.615850e-47
Msa0320540	Msa0834130	0.813222	2.855768e-51	-8.615850e-47
Msa0330270	Msa0834130	0.817317	3.511172e-52	-8.615850e-47
Msa0336270	Msa0834130	0.823736	1.179469e-53	-8.615850e-47
Msa0342200	Msa0834130	0.817085	3.959116e-52	-8.615850e-47
Msa0357380	Msa0834130	0.813524	2.450953e-51	-8.615850e-47
Msa0357390	Msa0834130	0.809869	1.528575e-50	-8.615850e-47
Msa0505390	Msa0834130	0.808398	3.158773e-50	-8.615850e-47
Msa0505660	Msa0834130	0.823464	1.365644e-53	-8.615850e-47
Msa0508200	Msa0834130	0.800299	1.538266e-48	-8.615850e-47
Msa0524050	Msa0834130	0.832078	1.160762e-55	-8.615850e-47
Msa0524090	Msa0834130	0.818420	1.978545e-52	-8.615850e-47
Msa0524120	Msa0834130	0.851772	7.139015e-61	-8.615850e-47
Msa0535450	Msa0834130	0.823779	1.152538e-53	-8.615850e-47
Msa0540320	Msa0834130	0.841454	4.704947e-58	-8.615850e-47
Msa0546740	Msa0834130	0.809466	1.866449e-50	-8.615850e-47
Msa0576420	Msa0834130	0.809232	2.094405e-50	-8.615850e-47
Msa0582110	Msa0834130	0.846247	2.449258e-59	-8.615850e-47
Msa0596100	Msa0834130	0.831830	1.336665e-55	-8.615850e-47
Msa0598250	Msa0834130	0.822925	1.824863e-53	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0834130	MtrunA17_Chr5g0399691	93.040	273	16	1	1	270	1	273	2.82e-157	440

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0834130	AT4G17540.1	45.714	280	123	5	10	285	28	282	3.96e-65	206

Find 101 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATCCATTTACTTTGAAATT+TGG	0.141463	5_4:-7178050	Msa0834130:CDS
TGTCCAAATTTCAAAGTAAA+TGG	0.158302	5_4:+7178047	None:intergenic
GCATTAGATGACAGGTATTT+AGG	0.201098	5_4:-7174373	Msa0834130:three_prime_UTR
GTTGGAAGCCCTTTAAATTT+AGG	0.210875	5_4:-7177981	Msa0834130:intron
GGACAAGTATTTACTGGTTT+TGG	0.211578	5_4:-7178029	Msa0834130:CDS
TAAGGGAGATCTCACGGTTT+AGG	0.233689	5_4:+7174513	None:intergenic
GCTTGCACGTTATCGCTTAA+AGG	0.246676	5_4:-7174542	Msa0834130:three_prime_UTR
AGAGTAGTACCTAAATTTAA+AGG	0.274370	5_4:+7177972	None:intergenic
CCTATACTATATCCTGTAAT+TGG	0.287565	5_4:+7174440	None:intergenic
AAGGGAGATCTCACGGTTTA+GGG	0.290126	5_4:+7174514	None:intergenic
ATGGGTTACTTACCAATTAC+AGG	0.311687	5_4:-7174452	Msa0834130:three_prime_UTR
CAGTCTGAGAAATTTGATTC+TGG	0.317467	5_4:-7178242	None:intergenic
GCCACAGGTGCAGTCAGTTC+TGG	0.319464	5_4:-7176488	Msa0834130:CDS
TTCATATAGTTGAGGAAGAT+TGG	0.320215	5_4:-7177576	Msa0834130:intron
AAATTTGGACAAGTATTTAC+TGG	0.322518	5_4:-7178035	Msa0834130:CDS
CAAGTTCTTCAATCATTTGC+TGG	0.323313	5_4:+7174749	None:intergenic
CTACAAGTTGCCCCTGCTAA+TGG	0.340138	5_4:-7174471	Msa0834130:three_prime_UTR
GTTGGATGTGGAGTTGGTTT+CGG	0.345001	5_4:-7177531	Msa0834130:CDS
CTTGCACGTTATCGCTTAAA+GGG	0.350496	5_4:-7174541	Msa0834130:three_prime_UTR
ACAAAGATGATGATGAAGTT+TGG	0.354809	5_4:-7176566	Msa0834130:CDS
ACCCGGATTAAACGGTAAAC+TGG	0.357165	5_4:+7176535	None:intergenic
AGAACTAGCTTCAAGTTTGC+TGG	0.358017	5_4:+7174672	None:intergenic
TCCCAGTTTACCGTTTAATC+CGG	0.367717	5_4:-7176537	Msa0834130:CDS
TACAAGTTGCCCCTGCTAAT+GGG	0.377837	5_4:-7174470	Msa0834130:three_prime_UTR
TTGGTTGGAGAGGAGTAAAA+TGG	0.387600	5_4:-7174267	Msa0834130:three_prime_UTR
AAACTTGAAGCTAGTTCTTC+AGG	0.400339	5_4:-7174667	Msa0834130:CDS
TCAACCAATGAACATTGGTT+TGG	0.414979	5_4:-7176405	Msa0834130:CDS
TACATGGGCAAGGGTCTGTT+TGG	0.419304	5_4:-7174286	Msa0834130:three_prime_UTR
TCTGCAAAATCCACTGTTGA+AGG	0.419732	5_4:-7176303	Msa0834130:CDS
AAAGCCAAACCAATGTTCAT+TGG	0.422194	5_4:+7176401	None:intergenic
TGCACCTGTGGCACTTTGTA+AGG	0.422373	5_4:+7176499	None:intergenic
GAGTAGTACCTAAATTTAAA+GGG	0.424503	5_4:+7177973	None:intergenic
GTGGGTGTTGGATGTGGAGT+TGG	0.433318	5_4:-7177537	Msa0834130:CDS
GGCAATGCACCCGGATTAAA+CGG	0.440473	5_4:+7176527	None:intergenic
CATTAGCAGGGGCAACTTGT+AGG	0.443920	5_4:+7174472	None:intergenic
ATCAAGTTATGAGTGCTACT+AGG	0.451482	5_4:-7177720	Msa0834130:CDS
TTTGGAATCGGTTGTGGAGT+TGG	0.458433	5_4:-7178011	Msa0834130:CDS
ATGAGAGCTTACTGATTCGC+TGG	0.460415	5_4:+7176265	None:intergenic
TCAAGTTATGAGTGCTACTA+GGG	0.471040	5_4:-7177719	Msa0834130:CDS
TTGGTAAGTAACCCATTAGC+AGG	0.472572	5_4:+7174459	None:intergenic
TGTGGAGTTGGTTTCGGTCA+TGG	0.472934	5_4:-7177525	Msa0834130:CDS
ACAGGACTTGCTGTGAAACC+TGG	0.477065	5_4:-7176715	Msa0834130:intron
CCAATTACAGGATATAGTAT+AGG	0.478483	5_4:-7174440	Msa0834130:three_prime_UTR
GAATCAATGGAAGCAATTGT+TGG	0.479648	5_4:+7178213	None:intergenic
GTTGGTTTCGGTCATGGTGT+TGG	0.481887	5_4:-7177519	Msa0834130:CDS
ATTGAAGTGGGTGTTGGATG+TGG	0.485704	5_4:-7177543	Msa0834130:CDS
TGGAGGAAAATGAGAAGCTT+CGG	0.486885	5_4:-7174726	Msa0834130:CDS
GCGGTTACAGCTTGAATCAA+TGG	0.487029	5_4:+7178200	None:intergenic
CCTCCTTCTCCCTTCAACAG+TGG	0.488432	5_4:+7176293	None:intergenic
TGGGCAAGGGTCTGTTTGGT+TGG	0.489899	5_4:-7174282	Msa0834130:three_prime_UTR
AAAAGATAAGAGTAGTATTG+AGG	0.494638	5_4:-7178113	Msa0834130:five_prime_UTR
AAGGGTCTGTTTGGTTGGAG+AGG	0.496557	5_4:-7174277	Msa0834130:three_prime_UTR
ACTTCAGCCGACTCTACATA+TGG	0.500649	5_4:-7176356	Msa0834130:CDS
TGTGGAGTTGGTATCGGTGT+TGG	0.502325	5_4:-7177999	Msa0834130:CDS
TTCGGTCATGGTGTTGGAGT+TGG	0.502955	5_4:-7177513	Msa0834130:intron
CCCAGTTTACCGTTTAATCC+GGG	0.504429	5_4:-7176536	Msa0834130:CDS
TGAATTTGATTCAACACCCC+AGG	0.508485	5_4:+7176697	None:intergenic
ACTAATTAGCATTAGATGAC+AGG	0.510013	5_4:-7174381	Msa0834130:three_prime_UTR
AAGAAAACAAAGAAGAAAGT+AGG	0.525920	5_4:-7174599	Msa0834130:exon
CACTCAAAACAATCAGTACA+TGG	0.527145	5_4:+7174625	None:intergenic
CTGCAAAATCCACTGTTGAA+GGG	0.528070	5_4:-7176302	Msa0834130:CDS
AAGAATATTGAAGTGGGTGT+TGG	0.528611	5_4:-7177549	Msa0834130:CDS
ATCGGTTGTGGAGTTGGTAT+CGG	0.538819	5_4:-7178005	Msa0834130:CDS
ATAAGAGTAGTATTGAGGAA+TGG	0.547460	5_4:-7178108	Msa0834130:exon
AGATGACAGGTATTTAGGAA+TGG	0.555064	5_4:-7174368	Msa0834130:three_prime_UTR
CCCGGATTAAACGGTAAACT+GGG	0.556288	5_4:+7176536	None:intergenic
GCAAATGATTGAAGAACTTG+TGG	0.559081	5_4:-7174746	Msa0834130:CDS
AAGGATGCTGGCAATGCACC+CGG	0.560291	5_4:+7176518	None:intergenic
CAGGACTTGCTGTGAAACCT+GGG	0.561209	5_4:-7176714	Msa0834130:CDS
AGAGAACAAAATACTTCAGA+TGG	0.561726	5_4:-7174920	Msa0834130:intron
ATAATATAGTAAAAGATACA+TGG	0.562231	5_4:-7174302	Msa0834130:three_prime_UTR
TAGTAAAAGATACATGGGCA+AGG	0.571477	5_4:-7174296	Msa0834130:three_prime_UTR
TCCAGAACTGACTGCACCTG+TGG	0.578803	5_4:+7176487	None:intergenic
TGGTAAGTAACCCATTAGCA+GGG	0.583261	5_4:+7174460	None:intergenic
CTAGCTTCAAGTTTGCTGGA+TGG	0.583563	5_4:+7174676	None:intergenic
TGAGAGCTTACTGATTCGCT+GGG	0.586043	5_4:+7176266	None:intergenic
GTGGCACTTTGTAAGGATGC+TGG	0.588800	5_4:+7176506	None:intergenic
AATCCACTGTTGAAGGGAGA+AGG	0.589188	5_4:-7176296	Msa0834130:CDS
GCATCCTTACAAAGTGCCAC+AGG	0.589278	5_4:-7176503	Msa0834130:CDS
TCAGCTGATCAGTTATCTCA+AGG	0.590242	5_4:-7176440	Msa0834130:intron
GTTGGAGAGGAGTAAAATGG+AGG	0.596132	5_4:-7174264	None:intergenic
ATATGGCAGTCGAACTGAGA+AGG	0.597072	5_4:-7176339	Msa0834130:CDS
TGGAGCTAAGAATATTGAAG+TGG	0.597204	5_4:-7177556	Msa0834130:CDS
CCACTGTTGAAGGGAGAAGG+AGG	0.603974	5_4:-7176293	Msa0834130:CDS
GGAGCTAAGAATATTGAAGT+GGG	0.605534	5_4:-7177555	Msa0834130:CDS
AGTAAAAGATACATGGGCAA+GGG	0.606431	5_4:-7174295	Msa0834130:three_prime_UTR
AATGATTGAAGAACTTGTGG+AGG	0.616671	5_4:-7174743	Msa0834130:CDS
TAATATAGTAAAAGATACAT+GGG	0.620520	5_4:-7174301	Msa0834130:three_prime_UTR
CTGTTGAAGGGAGAAGGAGG+AGG	0.623525	5_4:-7176290	Msa0834130:CDS
TCGACTGCCATATGTAGAGT+CGG	0.624499	5_4:+7176349	None:intergenic
GGGTCTCAACCAATGAACAT+TGG	0.632984	5_4:-7176410	Msa0834130:CDS
GCTTCGGCAGATACTAGTGA+AGG	0.640377	5_4:-7174710	Msa0834130:CDS
TGTTGCAGTGAGTTGAGTTG+AGG	0.643118	5_4:+7178155	None:intergenic
CTCATAACTTGATTCAACAT+CGG	0.655826	5_4:+7177730	None:intergenic
TTGCACGTTATCGCTTAAAG+GGG	0.660290	5_4:-7174540	Msa0834130:three_prime_UTR
AGGGAGATCTCACGGTTTAG+GGG	0.678296	5_4:+7174515	None:intergenic
GGTAAGTAACCCATTAGCAG+GGG	0.690059	5_4:+7174461	None:intergenic
AGGACTTGCTGTGAAACCTG+GGG	0.707838	5_4:-7176713	Msa0834130:CDS
CAAGTTATGAGTGCTACTAG+GGG	0.719794	5_4:-7177718	Msa0834130:CDS
ATCGTATTCGTAGAACGTGG+CGG	0.735634	5_4:+7178181	None:intergenic
AGCATCGTATTCGTAGAACG+TGG	0.739027	5_4:+7178178	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATATATATATATATATAT+TGG	+	chr5_4:7177421-7177440	None:intergenic	0.0%
!!	AAAAAAAAAAAAAGTGAAAA+AGG	+	chr5_4:7174663-7174682	None:intergenic	10.0%
!!!	AATTTTACAAAATACTATTT+TGG	+	chr5_4:7176654-7176673	None:intergenic	10.0%
!!!	TATTGTTATTTTTTTCAAAA+CGG	-	chr5_4:7176821-7176840	Msa0834130:intron	10.0%
!!!	ATTATTTCTTAATTTTTCTT+GGG	+	chr5_4:7178177-7178196	None:intergenic	10.0%
!!!	TATTATTTCTTAATTTTTCT+TGG	+	chr5_4:7178178-7178197	None:intergenic	10.0%
!!!	TAACTTTAAAAAGTGTATTT+AGG	-	chr5_4:7175074-7175093	Msa0834130:intron	15.0%
!!!	AACTTTAAAAAGTGTATTTA+GGG	-	chr5_4:7175075-7175094	Msa0834130:intron	15.0%
!!!	TTTTGAATTATTGATCATTT+AGG	-	chr5_4:7175101-7175120	Msa0834130:intron	15.0%
!!!	TTTTCTTTTATTATTATGAC+AGG	-	chr5_4:7175761-7175780	Msa0834130:intron	15.0%
!!!	TTGTTTCTTTATATACTTTT+TGG	-	chr5_4:7177266-7177285	Msa0834130:intron	15.0%
!!!	TGTTTATTTATTTTTTCCTA+TGG	-	chr5_4:7177705-7177724	Msa0834130:CDS	15.0%
!!	ATAATATAGTAAAAGATACA+TGG	-	chr5_4:7178192-7178211	Msa0834130:five_prime_UTR	15.0%
!!	TAATATAGTAAAAGATACAT+GGG	-	chr5_4:7178193-7178212	Msa0834130:five_prime_UTR	15.0%
!!!	AATCCATTTACTTTGAAATT+TGG	-	chr5_4:7174444-7174463	Msa0834130:three_prime_UTR	20.0%
!!!	CGAATTTTTTTAGATCATTT+AGG	-	chr5_4:7175154-7175173	Msa0834130:intron	20.0%
!!!	CATAATATTGAAAATTTTCC+AGG	-	chr5_4:7175186-7175205	Msa0834130:intron	20.0%
!!!	ATCTATTCATGAGTTTTTTT+GGG	+	chr5_4:7176677-7176696	None:intergenic	20.0%
!!	TTTCAAATATCACATTTGTT+TGG	-	chr5_4:7177027-7177046	Msa0834130:intron	20.0%
!!!	TTATTTTTTCCTATGGTTAT+AGG	-	chr5_4:7177712-7177731	Msa0834130:CDS	20.0%
!	AAAAGATAAGAGTAGTATTG+AGG	-	chr5_4:7174381-7174400	Msa0834130:three_prime_UTR	25.0%
!	TGTCCAAATTTCAAAGTAAA+TGG	+	chr5_4:7174450-7174469	None:intergenic	25.0%
!	AAATTTGGACAAGTATTTAC+TGG	-	chr5_4:7174459-7174478	Msa0834130:three_prime_UTR	25.0%
!	GAGTAGTACCTAAATTTAAA+GGG	+	chr5_4:7174524-7174543	None:intergenic	25.0%
!	AGAGTAGTACCTAAATTTAA+AGG	+	chr5_4:7174525-7174544	None:intergenic	25.0%
!	AATCCATAGTAATGTGAAAT+TGG	+	chr5_4:7174570-7174589	None:intergenic	25.0%
!	TTTCACATTACTATGGATTT+CGG	-	chr5_4:7174571-7174590	Msa0834130:three_prime_UTR	25.0%
!	AAAAGGTAATAAGAGATACA+TGG	+	chr5_4:7174646-7174665	None:intergenic	25.0%
!!!	TCTTATTCTGAATTTGTTGT+AGG	-	chr5_4:7174734-7174753	Msa0834130:CDS	25.0%
!!!	ACTTGTTTTTCATATAGTTG+AGG	-	chr5_4:7174910-7174929	Msa0834130:intron	25.0%
!	TGAATTATTGATCATTTAGG+AGG	-	chr5_4:7175104-7175123	Msa0834130:intron	25.0%
!!	AATATTGAAAATTTTCCAGG+AGG	-	chr5_4:7175189-7175208	Msa0834130:intron	25.0%
!	ATCTATGATCTATAATACAC+AGG	+	chr5_4:7175249-7175268	None:intergenic	25.0%
!	ATAACAGTTCATAAAGCATA+AGG	-	chr5_4:7175338-7175357	Msa0834130:intron	25.0%
!	TAACAGTTCATAAAGCATAA+GGG	-	chr5_4:7175339-7175358	Msa0834130:intron	25.0%
!!!	GTTTTTTAATATACAGTTCG+AGG	+	chr5_4:7175460-7175479	None:intergenic	25.0%
!	AAAGAAAATATGGAAAGGAT+GGG	+	chr5_4:7175651-7175670	None:intergenic	25.0%
!	TAAAGAAAATATGGAAAGGA+TGG	+	chr5_4:7175652-7175671	None:intergenic	25.0%
!	ATGAAGTGGTAAAGAAAATA+TGG	+	chr5_4:7175661-7175680	None:intergenic	25.0%
!!	ATTTTCTTTACCACTTCATA+TGG	-	chr5_4:7175662-7175681	Msa0834130:intron	25.0%
!!	TTTTCTTTACCACTTCATAT+GGG	-	chr5_4:7175663-7175682	Msa0834130:intron	25.0%
!	TTAAACATGAGATACACAAA+AGG	+	chr5_4:7175883-7175902	None:intergenic	25.0%
!	TAAAAAATCCTCATTCCAAA+TGG	+	chr5_4:7176468-7176487	None:intergenic	25.0%
!!!	ATTTGGAATGAGGATTTTTT+AGG	-	chr5_4:7176467-7176486	Msa0834130:CDS	25.0%
!!!	TTTGGAATGAGGATTTTTTA+GGG	-	chr5_4:7176468-7176487	Msa0834130:CDS	25.0%
!!!	CATCTATTCATGAGTTTTTT+TGG	+	chr5_4:7176678-7176697	None:intergenic	25.0%
!	AAAAACTCATGAATAGATGT+TGG	-	chr5_4:7176679-7176698	Msa0834130:intron	25.0%
!	AAAACTCATGAATAGATGTT+GGG	-	chr5_4:7176680-7176699	Msa0834130:intron	25.0%
!!	TTAAATTTTCTTGAGACAGA+GGG	+	chr5_4:7177068-7177087	None:intergenic	25.0%
!	CTCAAGAAAATTTAAGTACA+AGG	-	chr5_4:7177073-7177092	Msa0834130:intron	25.0%
!!	TGATATTTAAGATACATTGC+AGG	-	chr5_4:7177535-7177554	Msa0834130:CDS	25.0%
!	AAGAAAACAAAGAAGAAAGT+AGG	-	chr5_4:7177895-7177914	Msa0834130:intron	25.0%
!!!	AGATCTCCCTTAATTTTTTT+GGG	-	chr5_4:7177992-7178011	Msa0834130:CDS	25.0%
!	ACAAGACCCAAAAAAATTAA+GGG	+	chr5_4:7178001-7178020	None:intergenic	25.0%
!	AACAAGACCCAAAAAAATTA+AGG	+	chr5_4:7178002-7178021	None:intergenic	25.0%
!	ATAAGAGTAGTATTGAGGAA+TGG	-	chr5_4:7174386-7174405	Msa0834130:three_prime_UTR	30.0%
	GGAATGGAAAACAAAAATGA+AGG	-	chr5_4:7174402-7174421	Msa0834130:three_prime_UTR	30.0%
!!!	GTATTTACTGGTTTTGGAAT+CGG	-	chr5_4:7174471-7174490	Msa0834130:three_prime_UTR	30.0%
	AATTGGAAGAGAATAAGGAA+GGG	+	chr5_4:7174553-7174572	None:intergenic	30.0%
	AAATTGGAAGAGAATAAGGA+AGG	+	chr5_4:7174554-7174573	None:intergenic	30.0%
	TGTGAAATTGGAAGAGAATA+AGG	+	chr5_4:7174558-7174577	None:intergenic	30.0%
	CTTCCAATTTCACATTACTA+TGG	-	chr5_4:7174564-7174583	Msa0834130:three_prime_UTR	30.0%
!!!	TTTTTGAGTAGGGGAATTTT+GGG	+	chr5_4:7174623-7174642	None:intergenic	30.0%
!!!	AGATACATGGTTTTTGAGTA+GGG	+	chr5_4:7174633-7174652	None:intergenic	30.0%
	CTCATAACTTGATTCAACAT+CGG	+	chr5_4:7174767-7174786	None:intergenic	30.0%
	TTCATATAGTTGAGGAAGAT+TGG	-	chr5_4:7174918-7174937	Msa0834130:intron	30.0%
!	TGGTTTGAAAACCATTTTCA+TGG	+	chr5_4:7175290-7175309	None:intergenic	30.0%
	AACAGTTCATAAAGCATAAG+GGG	-	chr5_4:7175340-7175359	Msa0834130:intron	30.0%
	GTGGTAAAGAAAATATGGAA+AGG	+	chr5_4:7175656-7175675	None:intergenic	30.0%
	TGAAGAATGTTCACAAAACA+TGG	+	chr5_4:7175726-7175745	None:intergenic	30.0%
!	ACAAAGATGATGATGAAGTT+TGG	-	chr5_4:7175928-7175947	Msa0834130:intron	30.0%
	CACTTTATGCTTCACATAAA+TGG	-	chr5_4:7176369-7176388	Msa0834130:CDS	30.0%
!!!	AGGATTTTTTAGGGCTATAA+GGG	-	chr5_4:7176477-7176496	Msa0834130:CDS	30.0%
!	GCAAGAATCAAATTTTTCCT+AGG	+	chr5_4:7176605-7176624	None:intergenic	30.0%
!!!	CATACTTTTCCAAAAGTGTT+TGG	-	chr5_4:7176624-7176643	Msa0834130:intron	30.0%
!	TTTGGAAAACCAAACACTTT+TGG	+	chr5_4:7176636-7176655	None:intergenic	30.0%
	AAACTCATGAATAGATGTTG+GGG	-	chr5_4:7176681-7176700	Msa0834130:intron	30.0%
!!!	TTTTGGCGATAATGGAATTT+GGG	-	chr5_4:7176704-7176723	Msa0834130:CDS	30.0%
!!!	TTTTTTTCAAAACGGTATCC+TGG	-	chr5_4:7176829-7176848	Msa0834130:intron	30.0%
!!!	TTTTTTCAAAACGGTATCCT+GGG	-	chr5_4:7176830-7176849	Msa0834130:intron	30.0%
!	TAAATTTTCTTGAGACAGAG+GGG	+	chr5_4:7177067-7177086	None:intergenic	30.0%
!	CTTAAATTTTCTTGAGACAG+AGG	+	chr5_4:7177069-7177088	None:intergenic	30.0%
	TTGTTGAGACTGGAAAAATA+TGG	-	chr5_4:7177125-7177144	Msa0834130:intron	30.0%
!!	TACTTTTTGGTGATTAACTG+TGG	-	chr5_4:7177279-7177298	Msa0834130:intron	30.0%
	AGAGAACAAAATACTTCAGA+TGG	-	chr5_4:7177574-7177593	Msa0834130:intron	30.0%
	GTTTCAAAACCTATAACCAT+AGG	+	chr5_4:7177724-7177743	None:intergenic	30.0%
	AAAAATTAAGGGAGATCTCA+CGG	+	chr5_4:7177990-7178009	None:intergenic	30.0%
!!!	GAGATCTCCCTTAATTTTTT+TGG	-	chr5_4:7177991-7178010	Msa0834130:CDS	30.0%
	CCTATACTATATCCTGTAAT+TGG	+	chr5_4:7178057-7178076	None:intergenic	30.0%
	CCAATTACAGGATATAGTAT+AGG	-	chr5_4:7178054-7178073	Msa0834130:CDS	30.0%
	ACTAATTAGCATTAGATGAC+AGG	-	chr5_4:7178113-7178132	Msa0834130:five_prime_UTR	30.0%
	GAATCAATGGAAGCAATTGT+TGG	+	chr5_4:7174284-7174303	None:intergenic	35.0%
!!!	GGACAAGTATTTACTGGTTT+TGG	-	chr5_4:7174465-7174484	Msa0834130:three_prime_UTR	35.0%
	GTTGGAAGCCCTTTAAATTT+AGG	-	chr5_4:7174513-7174532	Msa0834130:three_prime_UTR	35.0%
!!	TTTTGAGTAGGGGAATTTTG+GGG	+	chr5_4:7174622-7174641	None:intergenic	35.0%
!!!	GTTTTTGAGTAGGGGAATTT+TGG	+	chr5_4:7174624-7174643	None:intergenic	35.0%
!!!	GATACATGGTTTTTGAGTAG+GGG	+	chr5_4:7174632-7174651	None:intergenic	35.0%
!!!	GAGATACATGGTTTTTGAGT+AGG	+	chr5_4:7174634-7174653	None:intergenic	35.0%
!	ATCAAGTTATGAGTGCTACT+AGG	-	chr5_4:7174774-7174793	Msa0834130:intron	35.0%
!	TCAAGTTATGAGTGCTACTA+GGG	-	chr5_4:7174775-7174794	Msa0834130:intron	35.0%
	TGGAGCTAAGAATATTGAAG+TGG	-	chr5_4:7174938-7174957	Msa0834130:CDS	35.0%
	GGAGCTAAGAATATTGAAGT+GGG	-	chr5_4:7174939-7174958	Msa0834130:CDS	35.0%
	AAGAATATTGAAGTGGGTGT+TGG	-	chr5_4:7174945-7174964	Msa0834130:intron	35.0%
!	TTGAAAATTTTCCAGGAGGT+AGG	-	chr5_4:7175193-7175212	Msa0834130:intron	35.0%
	AGTTATGCACTCCATGAAAA+TGG	-	chr5_4:7175276-7175295	Msa0834130:intron	35.0%
!	AAATGGTTTTCAAACCACCT+CGG	-	chr5_4:7175293-7175312	Msa0834130:intron	35.0%
	TTGAAGCAGAAGATGTTGTT+AGG	-	chr5_4:7175532-7175551	Msa0834130:intron	35.0%
	TGAAGCAGAAGATGTTGTTA+GGG	-	chr5_4:7175533-7175552	Msa0834130:intron	35.0%
	AAATATGGAAAGGATGGGAT+AGG	+	chr5_4:7175646-7175665	None:intergenic	35.0%
	CATCTTTGTCATTGTCATCT+AGG	+	chr5_4:7175917-7175936	None:intergenic	35.0%
	AGTTATCTCAAGGTCTAGTA+GGG	-	chr5_4:7176064-7176083	Msa0834130:intron	35.0%
!	TCAACCAATGAACATTGGTT+TGG	-	chr5_4:7176089-7176108	Msa0834130:intron	35.0%
	AAAGCCAAACCAATGTTCAT+TGG	+	chr5_4:7176096-7176115	None:intergenic	35.0%
	GCTTCAATGATCAGAAGAAA+AGG	+	chr5_4:7176291-7176310	None:intergenic	35.0%
!!	ACCATTTTCCATTTGGAATG+AGG	-	chr5_4:7176457-7176476	Msa0834130:CDS	35.0%
	TCCTCATTCCAAATGGAAAA+TGG	+	chr5_4:7176461-7176480	None:intergenic	35.0%
!!!	GAGGATTTTTTAGGGCTATA+AGG	-	chr5_4:7176476-7176495	Msa0834130:CDS	35.0%
!!!	ATTTTTTAGGGCTATAAGGG+AGG	-	chr5_4:7176480-7176499	Msa0834130:CDS	35.0%
!	ATGAATAGATGTTGGGGTTT+TGG	-	chr5_4:7176687-7176706	Msa0834130:CDS	35.0%
!!!	GTTTTGGCGATAATGGAATT+TGG	-	chr5_4:7176703-7176722	Msa0834130:CDS	35.0%
!	AATGTTCGCGTATTGTTTTC+AGG	+	chr5_4:7176966-7176985	None:intergenic	35.0%
	GCTGTTTAAATTGTTGAGAC+TGG	-	chr5_4:7177115-7177134	Msa0834130:intron	35.0%
	AAATATGGTTATCTGCTGAG+AGG	-	chr5_4:7177140-7177159	Msa0834130:intron	35.0%
!	CTGTGTGACAATTTTAAACG+AGG	-	chr5_4:7177231-7177250	Msa0834130:intron	35.0%
	TGGTGATTAACTGTGGTATT+TGG	-	chr5_4:7177286-7177305	Msa0834130:intron	35.0%
	GGTGATTAACTGTGGTATTT+GGG	-	chr5_4:7177287-7177306	Msa0834130:intron	35.0%
	CAGAAAAAACTCACGAAAGA+GGG	+	chr5_4:7177489-7177508	None:intergenic	35.0%
	TCAGAAAAAACTCACGAAAG+AGG	+	chr5_4:7177490-7177509	None:intergenic	35.0%
!	ATTTAAGATACATTGCAGGC+TGG	-	chr5_4:7177539-7177558	Msa0834130:CDS	35.0%
!!!	CTTGTTGATTTTTTTTGCGC+TGG	-	chr5_4:7177659-7177678	Msa0834130:intron	35.0%
	CAAGTTCTTCAATCATTTGC+TGG	+	chr5_4:7177748-7177767	None:intergenic	35.0%
	GCAAATGATTGAAGAACTTG+TGG	-	chr5_4:7177748-7177767	Msa0834130:intron	35.0%
	AATGATTGAAGAACTTGTGG+AGG	-	chr5_4:7177751-7177770	Msa0834130:intron	35.0%
!	AAACTTGAAGCTAGTTCTTC+AGG	-	chr5_4:7177827-7177846	Msa0834130:intron	35.0%
	CACTCAAAACAATCAGTACA+TGG	+	chr5_4:7177872-7177891	None:intergenic	35.0%
!	ATGGGTTACTTACCAATTAC+AGG	-	chr5_4:7178042-7178061	Msa0834130:CDS	35.0%
	GCATTAGATGACAGGTATTT+AGG	-	chr5_4:7178121-7178140	Msa0834130:five_prime_UTR	35.0%
	AGATGACAGGTATTTAGGAA+TGG	-	chr5_4:7178126-7178145	Msa0834130:five_prime_UTR	35.0%
	TAGTAAAAGATACATGGGCA+AGG	-	chr5_4:7178198-7178217	Msa0834130:five_prime_UTR	35.0%
	AGTAAAAGATACATGGGCAA+GGG	-	chr5_4:7178199-7178218	Msa0834130:five_prime_UTR	35.0%
	GAAGAGAATAAGGAAGGGAA+AGG	+	chr5_4:7174548-7174567	None:intergenic	40.0%
!	CAAGTTATGAGTGCTACTAG+GGG	-	chr5_4:7174776-7174795	Msa0834130:intron	40.0%
!!	ATGCATTTTCTGGTGTTAGC+AGG	-	chr5_4:7174807-7174826	Msa0834130:intron	40.0%
!	CAAGCCTTATCGCTTTAAGT+GGG	-	chr5_4:7175395-7175414	Msa0834130:intron	40.0%
!	AAGCCTTATCGCTTTAAGTG+GGG	-	chr5_4:7175396-7175415	Msa0834130:intron	40.0%
	TCTGCAGTATCCAGAGATTT+AGG	+	chr5_4:7175577-7175596	None:intergenic	40.0%
	TCTTTACCACTTCATATGGG+TGG	-	chr5_4:7175666-7175685	Msa0834130:intron	40.0%
	CATAATCCACCCATATGAAG+TGG	+	chr5_4:7175675-7175694	None:intergenic	40.0%
	TGAATTTGATTCAACACCCC+AGG	+	chr5_4:7175800-7175819	None:intergenic	40.0%
	TCCCAGTTTACCGTTTAATC+CGG	-	chr5_4:7175957-7175976	Msa0834130:intron	40.0%
	TCAGCTGATCAGTTATCTCA+AGG	-	chr5_4:7176054-7176073	Msa0834130:intron	40.0%
	CAGTTATCTCAAGGTCTAGT+AGG	-	chr5_4:7176063-7176082	Msa0834130:intron	40.0%
	TCTGCAAAATCCACTGTTGA+AGG	-	chr5_4:7176191-7176210	Msa0834130:intron	40.0%
	CTGCAAAATCCACTGTTGAA+GGG	-	chr5_4:7176192-7176211	Msa0834130:intron	40.0%
!	TGCTGAGACCATTTTCCATT+TGG	-	chr5_4:7176450-7176469	Msa0834130:CDS	40.0%
	GAGAAGGTAGGCTAAAATTC+AGG	-	chr5_4:7176531-7176550	Msa0834130:CDS	40.0%
!!	GTGCCATTTGACTTGTATCA+CGG	-	chr5_4:7176577-7176596	Msa0834130:CDS	40.0%
!!!	CTTGGTTTTTGGCCAAAACA+GGG	-	chr5_4:7176884-7176903	Msa0834130:intron	40.0%
!!!	TTGGTTTTTGGCCAAAACAG+GGG	-	chr5_4:7176885-7176904	Msa0834130:intron	40.0%
	AAAAACTCACGAAAGAGGGA+AGG	+	chr5_4:7177485-7177504	None:intergenic	40.0%
	TTCCAAGTCTATCAGTGTTG+AGG	+	chr5_4:7177616-7177635	None:intergenic	40.0%
	TGGAGGAAAATGAGAAGCTT+CGG	-	chr5_4:7177768-7177787	Msa0834130:intron	40.0%
!	AGAACTAGCTTCAAGTTTGC+TGG	+	chr5_4:7177825-7177844	None:intergenic	40.0%
	CTTGCACGTTATCGCTTAAA+GGG	-	chr5_4:7177953-7177972	Msa0834130:intron	40.0%
	TTGCACGTTATCGCTTAAAG+GGG	-	chr5_4:7177954-7177973	Msa0834130:intron	40.0%
	TGGTAAGTAACCCATTAGCA+GGG	+	chr5_4:7178037-7178056	None:intergenic	40.0%
	TTGGTAAGTAACCCATTAGC+AGG	+	chr5_4:7178038-7178057	None:intergenic	40.0%
	GCGGTTACAGCTTGAATCAA+TGG	+	chr5_4:7174297-7174316	None:intergenic	45.0%
	ATCGTATTCGTAGAACGTGG+CGG	+	chr5_4:7174316-7174335	None:intergenic	45.0%
	AGCATCGTATTCGTAGAACG+TGG	+	chr5_4:7174319-7174338	None:intergenic	45.0%
!	TGTTGCAGTGAGTTGAGTTG+AGG	+	chr5_4:7174342-7174361	None:intergenic	45.0%
!!!	ACTGGTTTTGGAATCGGTTG+TGG	-	chr5_4:7174477-7174496	Msa0834130:three_prime_UTR	45.0%
!	TTTGGAATCGGTTGTGGAGT+TGG	-	chr5_4:7174483-7174502	Msa0834130:three_prime_UTR	45.0%
!!	ATCGGTTGTGGAGTTGGTAT+CGG	-	chr5_4:7174489-7174508	Msa0834130:three_prime_UTR	45.0%
!!	GGGGAATTTTGGGGAGATTT+GGG	+	chr5_4:7174613-7174632	None:intergenic	45.0%
!!	AGGGGAATTTTGGGGAGATT+TGG	+	chr5_4:7174614-7174633	None:intergenic	45.0%
!	GGTGCAACTGATGCATTTTC+TGG	-	chr5_4:7174797-7174816	Msa0834130:intron	45.0%
!!	ATTGAAGTGGGTGTTGGATG+TGG	-	chr5_4:7174951-7174970	Msa0834130:intron	45.0%
!	GTTGGATGTGGAGTTGGTTT+CGG	-	chr5_4:7174963-7174982	Msa0834130:intron	45.0%
!	TTCCAGGAGGTAGGTTTTTG+TGG	-	chr5_4:7175202-7175221	Msa0834130:intron	45.0%
	TTCCACAAAAACCTACCTCC+TGG	+	chr5_4:7175207-7175226	None:intergenic	45.0%
	CCACTTAAAGCGATAAGGCT+TGG	+	chr5_4:7175397-7175416	None:intergenic	45.0%
!	CCAAGCCTTATCGCTTTAAG+TGG	-	chr5_4:7175394-7175413	Msa0834130:intron	45.0%
!	CTTATCGCTTTAAGTGGGGT+CGG	-	chr5_4:7175400-7175419	Msa0834130:intron	45.0%
	GTTCGAGGCATAGAACACTA+TGG	+	chr5_4:7175445-7175464	None:intergenic	45.0%
	CCCGGATTAAACGGTAAACT+GGG	+	chr5_4:7175961-7175980	None:intergenic	45.0%
	CCCAGTTTACCGTTTAATCC+GGG	-	chr5_4:7175958-7175977	Msa0834130:intron	45.0%
	ACCCGGATTAAACGGTAAAC+TGG	+	chr5_4:7175962-7175981	None:intergenic	45.0%
	GGGTCTCAACCAATGAACAT+TGG	-	chr5_4:7176084-7176103	Msa0834130:intron	45.0%
!!	GTCGGCTGAAGTGTCTTTTA+TGG	+	chr5_4:7176130-7176149	None:intergenic	45.0%
!	ACTTCAGCCGACTCTACATA+TGG	-	chr5_4:7176138-7176157	Msa0834130:intron	45.0%
	TCGACTGCCATATGTAGAGT+CGG	+	chr5_4:7176148-7176167	None:intergenic	45.0%
	ATATGGCAGTCGAACTGAGA+AGG	-	chr5_4:7176155-7176174	Msa0834130:intron	45.0%
	AATCCACTGTTGAAGGGAGA+AGG	-	chr5_4:7176198-7176217	Msa0834130:intron	45.0%
!!	TGAGAGCTTACTGATTCGCT+GGG	+	chr5_4:7176231-7176250	None:intergenic	45.0%
!!	ATGAGAGCTTACTGATTCGC+TGG	+	chr5_4:7176232-7176251	None:intergenic	45.0%
	TAGTGTGGAGATGAGAAGGT+AGG	-	chr5_4:7176519-7176538	Msa0834130:CDS	45.0%
	TCAAATGGCACAAAGCCCTT+TGG	+	chr5_4:7176568-7176587	None:intergenic	45.0%
	GAGCCGTGATACAAGTCAAA+TGG	+	chr5_4:7176583-7176602	None:intergenic	45.0%
!!	TGTTGGGGTTTTGGCGATAA+TGG	-	chr5_4:7176696-7176715	Msa0834130:CDS	45.0%
!!	TACTGGTATGGTGGGTAGAA+AGG	-	chr5_4:7176769-7176788	Msa0834130:intron	45.0%
	GGTAGAAAGGATGGATGAGT+TGG	-	chr5_4:7176782-7176801	Msa0834130:intron	45.0%
!!	GATGGATGAGTTGGTCATGT+TGG	-	chr5_4:7176791-7176810	Msa0834130:intron	45.0%
!!	GATGAGTTGGTCATGTTGGT+GGG	-	chr5_4:7176795-7176814	Msa0834130:intron	45.0%
!	TGGGGTTTTCTTTAACACCC+AGG	+	chr5_4:7176850-7176869	None:intergenic	45.0%
!!	CTCCCTATGAGCTTGGTTTT+TGG	-	chr5_4:7176873-7176892	Msa0834130:intron	45.0%
	GGCCAAAAACCAAGCTCATA+GGG	+	chr5_4:7176878-7176897	None:intergenic	45.0%
	TGGCCAAAAACCAAGCTCAT+AGG	+	chr5_4:7176879-7176898	None:intergenic	45.0%
!!!	GCTTGGTTTTTGGCCAAAAC+AGG	-	chr5_4:7176883-7176902	Msa0834130:intron	45.0%
!	GATGCAACATTCCCCTGTTT+TGG	+	chr5_4:7176899-7176918	None:intergenic	45.0%
	TAACAACAGACGATGCCTAC+AGG	+	chr5_4:7177209-7177228	None:intergenic	45.0%
	GTCCTCAACACTGATAGACT+TGG	-	chr5_4:7177611-7177630	Msa0834130:intron	45.0%
!!	CTAGCTTCAAGTTTGCTGGA+TGG	+	chr5_4:7177821-7177840	None:intergenic	45.0%
	GCTTGCACGTTATCGCTTAA+AGG	-	chr5_4:7177952-7177971	Msa0834130:intron	45.0%
	AAGGGAGATCTCACGGTTTA+GGG	+	chr5_4:7177983-7178002	None:intergenic	45.0%
	TAAGGGAGATCTCACGGTTT+AGG	+	chr5_4:7177984-7178003	None:intergenic	45.0%
	TACAAGTTGCCCCTGCTAAT+GGG	-	chr5_4:7178024-7178043	Msa0834130:CDS	45.0%
	GGTAAGTAACCCATTAGCAG+GGG	+	chr5_4:7178036-7178055	None:intergenic	45.0%
!!	TGTGGAGTTGGTATCGGTGT+TGG	-	chr5_4:7174495-7174514	Msa0834130:three_prime_UTR	50.0%
!	TGTGGAGTTGGTTTCGGTCA+TGG	-	chr5_4:7174969-7174988	Msa0834130:intron	50.0%
!	GTTGGTTTCGGTCATGGTGT+TGG	-	chr5_4:7174975-7174994	Msa0834130:intron	50.0%
!	TTCGGTCATGGTGTTGGAGT+TGG	-	chr5_4:7174981-7175000	Msa0834130:intron	50.0%
!	TTTTCAAACCACCTCGGCAC+TGG	-	chr5_4:7175299-7175318	Msa0834130:intron	50.0%
	CGACCCCACTTAAAGCGATA+AGG	+	chr5_4:7175402-7175421	None:intergenic	50.0%
	TTTAAGTGGGGTCGGCTACA+AGG	-	chr5_4:7175408-7175427	Msa0834130:intron	50.0%
!!	ACAGGACTTGCTGTGAAACC+TGG	-	chr5_4:7175779-7175798	Msa0834130:intron	50.0%
!!	CAGGACTTGCTGTGAAACCT+GGG	-	chr5_4:7175780-7175799	Msa0834130:intron	50.0%
!!	AGGACTTGCTGTGAAACCTG+GGG	-	chr5_4:7175781-7175800	Msa0834130:intron	50.0%
	GGCAATGCACCCGGATTAAA+CGG	+	chr5_4:7175970-7175989	None:intergenic	50.0%
	GTGGCACTTTGTAAGGATGC+TGG	+	chr5_4:7175991-7176010	None:intergenic	50.0%
!	GCATCCTTACAAAGTGCCAC+AGG	-	chr5_4:7175991-7176010	Msa0834130:intron	50.0%
	TGCACCTGTGGCACTTTGTA+AGG	+	chr5_4:7175998-7176017	None:intergenic	50.0%
!	CCACACTACGAGTTGGAACT+TGG	+	chr5_4:7176507-7176526	None:intergenic	50.0%
	CCAAGTTCCAACTCGTAGTG+TGG	-	chr5_4:7176504-7176523	Msa0834130:CDS	50.0%
	CTCATCTCCACACTACGAGT+TGG	+	chr5_4:7176514-7176533	None:intergenic	50.0%
	CTCGTAGTGTGGAGATGAGA+AGG	-	chr5_4:7176515-7176534	Msa0834130:CDS	50.0%
	TCAGGCAAAGTAGCCTCCAA+AGG	-	chr5_4:7176549-7176568	Msa0834130:CDS	50.0%
	CAGGCAAAGTAGCCTCCAAA+GGG	-	chr5_4:7176550-7176569	Msa0834130:CDS	50.0%
	AATGGCACAAAGCCCTTTGG+AGG	+	chr5_4:7176565-7176584	None:intergenic	50.0%
!	TGACTTGTATCACGGCTCCT+AGG	-	chr5_4:7176585-7176604	Msa0834130:intron	50.0%
	CACCACAATAGCAGCCATTG+CGG	+	chr5_4:7176745-7176764	None:intergenic	50.0%
!!	TGCTATTGTGGTGGTGCTAC+TGG	-	chr5_4:7176752-7176771	Msa0834130:intron	50.0%
!!	TTGTGGTGGTGCTACTGGTA+TGG	-	chr5_4:7176757-7176776	Msa0834130:intron	50.0%
!!	GGTATGGTGGGTAGAAAGGA+TGG	-	chr5_4:7176773-7176792	Msa0834130:intron	50.0%
!!	GGATGAGTTGGTCATGTTGG+TGG	-	chr5_4:7176794-7176813	Msa0834130:intron	50.0%
	CAAGCTCATAGGGAGAGATG+GGG	+	chr5_4:7176868-7176887	None:intergenic	50.0%
	CCAAGCTCATAGGGAGAGAT+GGG	+	chr5_4:7176869-7176888	None:intergenic	50.0%
	ACCAAGCTCATAGGGAGAGA+TGG	+	chr5_4:7176870-7176889	None:intergenic	50.0%
	TGCACATGTCCTCTTCCTGT+AGG	-	chr5_4:7177191-7177210	Msa0834130:intron	50.0%
	ACTCACGAAAGAGGGAAGGT+GGG	+	chr5_4:7177481-7177500	None:intergenic	50.0%
	AACTCACGAAAGAGGGAAGG+TGG	+	chr5_4:7177482-7177501	None:intergenic	50.0%
	GCTTCGGCAGATACTAGTGA+AGG	-	chr5_4:7177784-7177803	Msa0834130:intron	50.0%
	AGGGAGATCTCACGGTTTAG+GGG	+	chr5_4:7177982-7178001	None:intergenic	50.0%
	CATTAGCAGGGGCAACTTGT+AGG	+	chr5_4:7178025-7178044	None:intergenic	50.0%
	CTACAAGTTGCCCCTGCTAA+TGG	-	chr5_4:7178023-7178042	Msa0834130:CDS	50.0%
	TACATGGGCAAGGGTCTGTT+TGG	-	chr5_4:7178208-7178227	Msa0834130:five_prime_UTR	50.0%
	AAGGGTCTGTTTGGTTGGAG+AGG	-	chr5_4:7178217-7178236	Msa0834130:five_prime_UTR	50.0%
!!	GTGGGTGTTGGATGTGGAGT+TGG	-	chr5_4:7174957-7174976	Msa0834130:intron	55.0%
	AATTGTAGCCTCCAGTGCCG+AGG	+	chr5_4:7175313-7175332	None:intergenic	55.0%
!	TTGTTAGGGTGCCACAACCG+CGG	-	chr5_4:7175547-7175566	Msa0834130:intron	55.0%
	ATTTAGGCATGCCGCGGTTG+TGG	+	chr5_4:7175561-7175580	None:intergenic	55.0%
	CCAGAGATTTAGGCATGCCG+CGG	+	chr5_4:7175567-7175586	None:intergenic	55.0%
	AAGGATGCTGGCAATGCACC+CGG	+	chr5_4:7175979-7175998	None:intergenic	55.0%
	TCCAGAACTGACTGCACCTG+TGG	+	chr5_4:7176010-7176029	None:intergenic	55.0%
	CCTCCTTCTCCCTTCAACAG+TGG	+	chr5_4:7176204-7176223	None:intergenic	55.0%
	CCACTGTTGAAGGGAGAAGG+AGG	-	chr5_4:7176201-7176220	Msa0834130:intron	55.0%
	CTGTTGAAGGGAGAAGGAGG+AGG	-	chr5_4:7176204-7176223	Msa0834130:intron	55.0%
	ACAGCAATCGCAAGCCGCAA+TGG	-	chr5_4:7176728-7176747	Msa0834130:intron	55.0%
	AGCCGCAATGGCTGCTATTG+TGG	-	chr5_4:7176740-7176759	Msa0834130:intron	55.0%
	CGCAATGGCTGCTATTGTGG+TGG	-	chr5_4:7176743-7176762	Msa0834130:intron	55.0%
!!	TGGTGGTGCTACTGGTATGG+TGG	-	chr5_4:7176760-7176779	Msa0834130:intron	55.0%
!!	GGTGGTGCTACTGGTATGGT+GGG	-	chr5_4:7176761-7176780	Msa0834130:intron	55.0%
	CCCATCTCTCCCTATGAGCT+TGG	-	chr5_4:7176866-7176885	Msa0834130:intron	55.0%
	CAGACGATGCCTACAGGAAG+AGG	+	chr5_4:7177203-7177222	None:intergenic	55.0%
	CTCACGAAAGAGGGAAGGTG+GGG	+	chr5_4:7177480-7177499	None:intergenic	55.0%
	TGGGCAAGGGTCTGTTTGGT+TGG	-	chr5_4:7178212-7178231	Msa0834130:five_prime_UTR	55.0%
!	TCAAACCACCTCGGCACTGG+AGG	-	chr5_4:7175302-7175321	Msa0834130:intron	60.0%
	CCGCGGCATGCCTAAATCTC+TGG	-	chr5_4:7175564-7175583	Msa0834130:intron	60.0%
	GCCACAGGTGCAGTCAGTTC+TGG	-	chr5_4:7176006-7176025	Msa0834130:intron	60.0%
!	TGTAGCCTCCAGTGCCGAGG+TGG	+	chr5_4:7175310-7175329	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	7174267	7178249	7174267	ID=Msa0834130;Name=Msa0834130
chr5_4	mRNA	7174267	7178249	7174267	ID=Msa0834130-mRNA-1;Parent=Msa0834130;Name=Msa0834130-mRNA-1;_AED=0.18;_eAED=0.18;_QI=138\|0.85\|0.75\|1\|0.71\|0.75\|8\|333\|292
chr5_4	exon	7174267	7174782	7174267	ID=Msa0834130-mRNA-1:exon:3160;Parent=Msa0834130-mRNA-1
chr5_4	exon	7174921	7174959	7174921	ID=Msa0834130-mRNA-1:exon:3159;Parent=Msa0834130-mRNA-1
chr5_4	exon	7176277	7176431	7176277	ID=Msa0834130-mRNA-1:exon:3158;Parent=Msa0834130-mRNA-1
chr5_4	exon	7176441	7176597	7176441	ID=Msa0834130-mRNA-1:exon:3157;Parent=Msa0834130-mRNA-1
chr5_4	exon	7176684	7176733	7176684	ID=Msa0834130-mRNA-1:exon:3156;Parent=Msa0834130-mRNA-1
chr5_4	exon	7177514	7177589	7177514	ID=Msa0834130-mRNA-1:exon:3155;Parent=Msa0834130-mRNA-1
chr5_4	exon	7177672	7177760	7177672	ID=Msa0834130-mRNA-1:exon:3154;Parent=Msa0834130-mRNA-1
chr5_4	exon	7177982	7178249	7177982	ID=Msa0834130-mRNA-1:exon:3153;Parent=Msa0834130-mRNA-1
chr5_4	five_prime_UTR	7178112	7178249	7178112	ID=Msa0834130-mRNA-1:five_prime_utr;Parent=Msa0834130-mRNA-1
chr5_4	CDS	7177982	7178111	7177982	ID=Msa0834130-mRNA-1:cds;Parent=Msa0834130-mRNA-1
chr5_4	CDS	7177672	7177760	7177672	ID=Msa0834130-mRNA-1:cds;Parent=Msa0834130-mRNA-1
chr5_4	CDS	7177514	7177589	7177514	ID=Msa0834130-mRNA-1:cds;Parent=Msa0834130-mRNA-1
chr5_4	CDS	7176684	7176733	7176684	ID=Msa0834130-mRNA-1:cds;Parent=Msa0834130-mRNA-1
chr5_4	CDS	7176441	7176597	7176441	ID=Msa0834130-mRNA-1:cds;Parent=Msa0834130-mRNA-1
chr5_4	CDS	7176277	7176431	7176277	ID=Msa0834130-mRNA-1:cds;Parent=Msa0834130-mRNA-1
chr5_4	CDS	7174921	7174959	7174921	ID=Msa0834130-mRNA-1:cds;Parent=Msa0834130-mRNA-1
chr5_4	CDS	7174600	7174782	7174600	ID=Msa0834130-mRNA-1:cds;Parent=Msa0834130-mRNA-1
chr5_4	three_prime_UTR	7174267	7174599	7174267	ID=Msa0834130-mRNA-1:three_prime_utr;Parent=Msa0834130-mRNA-1

Gene Sequence

>Msa0834130

ATGGAAAACAAAAATGAAGGAAATGAAATTCGATTTTCGAATCCATTTACTTTGAAATTTGGACAAGTATTTACTGGTTTTGGAATCGGTTGTGGAGTTGGTATCGGTGTTGGAAGCCCTTTAAATTTAGGTGCAATACCGATGTTGAATCAAGTTATGAGTGCTACTAGGGGTGCAACTGATGCATTTTCTGGTGTTAGCAGGCATGTTAATACTTCTTTGAGGAAGATTGGAGCTAAGAATATTGAAGTGGGTGTTGGATGTGGAGTTGGTTTCGGTCATGGTGTTGGAGTTGGACTTGCTGTGAAACCTGGGGTGTTGAATCAAATTCAATCATGTCTCGTAATGACAATGACAAAGATGATGATGAAGTTTGGAATTACTCCCAGTTTACCGTTTAATCCGGGTGCATTGCCAGCATCCTTACAAAGTGCCACAGGTGCAGTCAGTTCTGGAAGCGTGACACAGTTAGCAACTAAATCAGCTGATCAGTTATCTCAAGGGTCTCAACCAATGAACATTGGTTTGGCTTTTGATAAAACTGCCATAAAAGACACTTCAGCCGACTCTACATATGGCAGTCGAACTGAGAAGGTTCTTAACAATTTTCTGCAAAATCCACTGTTGAAGGGAGAAGGAGGAGGACCCAGCGAATCAGCTGGACGTTTGATCACAGAGAACAAAATACTTCAGATGGTTTTGAAACACCAGCAAATGATTGAAGAACTTGTGGAGGAAAATGAGAAGCTTCGGCAGATACTAGTGAAGGAGCTGAAAGTTCCATCCAGCAAACTTGAAGCTAGTTCTTCAGGTAGAATTAGAAATAAGTTGCCATGTACTGATTGTTTTGAGTGTCGAAGAAAACAAAGAAGAAAGTAG

Protein sequence

>Msa0834130

MENKNEGNEIRFSNPFTLKFGQVFTGFGIGCGVGIGVGSPLNLGAIPMLNQVMSATRGATDAFSGVSRHVNTSLRKIGAKNIEVGVGCGVGFGHGVGVGLAVKPGVLNQIQSCLVMTMTKMMMKFGITPSLPFNPGALPASLQSATGAVSSGSVTQLATKSADQLSQGSQPMNIGLAFDKTAIKDTSADSTYGSRTEKVLNNFLQNPLLKGEGGGPSESAGRLITENKILQMVLKHQQMIEELVEENEKLRQILVKELKVPSSKLEASSSGRIRNKLPCTDCFECRRKQRRK*