AlfalfaGEDB | Msa1271200

M. sativa cultivar zhongmu-4 / Msa1271200

KEGG: K08237
GO: -
PFAM: PF00201.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1271200	XP_013443796.2	95.754	471	20	0	1	471	1	471	0.0	924

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1271200	sp\|Q9AR73\|HQGT_RAUSE	48.203	473	231	10	4	470	1	465	6.30e-148	433

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1271200	A0A396GIN6	95.754	471	20	0	1	471	1	471	0.0	924

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006700	Msa1271200	0.819593	1.070815e-52	-8.615850e-47
Msa0011720	Msa1271200	0.818631	1.772523e-52	-8.615850e-47
Msa0015100	Msa1271200	0.801887	7.284400e-49	-8.615850e-47
Msa0049770	Msa1271200	0.808067	3.715612e-50	-8.615850e-47
Msa0050720	Msa1271200	0.817016	4.102423e-52	-8.615850e-47
Msa0050760	Msa1271200	0.802511	5.418758e-49	-8.615850e-47
Msa0050770	Msa1271200	0.805073	1.591879e-49	-8.615850e-47
Msa0071240	Msa1271200	0.800197	1.613627e-48	-8.615850e-47
Msa0072000	Msa1271200	0.811896	5.566172e-51	-8.615850e-47
Msa0077390	Msa1271200	0.824064	9.882850e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1271200	MtrunA17_Chr8g0335141	95.754	471	20	0	1	471	1	471	0.0	924
Msa1271200	MtrunA17_Chr8g0335161	85.928	469	65	1	4	471	1	469	0.0	829
Msa1271200	MtrunA17_Chr6g0459311	72.766	470	123	3	4	471	8	474	0.0	704
Msa1271200	MtrunA17_Chr6g0486251	71.336	464	128	3	4	465	1	461	0.0	682
Msa1271200	MtrunA17_Chr6g0486201	71.336	464	128	3	4	465	1	461	0.0	682
Msa1271200	MtrunA17_Chr8g0361371	58.351	473	179	9	3	464	1	466	0.0	536
Msa1271200	MtrunA17_Chr8g0334831	55.809	482	186	10	4	465	1	475	1.53e-178	509
Msa1271200	MtrunA17_Chr7g0232411	46.414	474	242	7	4	471	1	468	3.46e-140	410
Msa1271200	MtrunA17_Chr7g0232391	46.835	474	240	7	4	471	1	468	5.76e-140	410
Msa1271200	MtrunA17_Chr7g0232341	45.781	474	244	8	4	470	1	468	2.24e-138	406

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1271200	AT4G01070.1	45.299	468	242	10	5	465	4	464	1.41e-133	394
Msa1271200	AT1G01420.2	45.923	466	235	12	8	465	10	466	8.10e-131	387
Msa1271200	AT1G01420.1	45.923	466	235	12	8	465	7	463	9.76e-131	387
Msa1271200	AT1G01390.1	45.923	466	234	12	8	464	7	463	4.69e-120	360
Msa1271200	AT1G01390.2	45.923	466	234	12	8	464	24	480	7.37e-120	360
Msa1271200	AT3G50740.1	37.967	482	268	12	3	465	1	470	1.43e-98	305
Msa1271200	AT5G26310.1	36.810	489	272	15	1	471	1	470	2.57e-96	299
Msa1271200	AT5G66690.1	36.864	472	265	13	1	457	1	454	6.28e-92	288
Msa1271200	AT2G18570.1	35.908	479	277	16	7	469	3	467	1.23e-82	263
Msa1271200	AT4G36770.1	35.683	454	264	13	11	450	6	445	2.22e-80	257

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Find 90 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGGCTAAGTCCAGGGGA+AGG	0.656957	8_3:+4663262	None:intergenic
AAGAAACAGAGAAAATGGAT+TGG	0.554526	8_3:-4662300	Msa1271200:CDS
AAGAAGTTGCTAACTGTGTA+AGG	0.451651	8_3:-4662049	Msa1271200:CDS
AATAGAGGCCACGCAATCAT+AGG	0.415417	8_3:+4662167	None:intergenic
AATTAAATGGCTAAGTCCAG+GGG	0.708782	8_3:+4663258	None:intergenic
AATTAGACTCATTTCTGTTA+AGG	0.345841	8_3:+4662573	None:intergenic
ACATAAAGAACAGAACCTGG+TGG	0.755413	8_3:+4662503	None:intergenic
ACATTAATTCAATGTGTTTA+AGG	0.145467	8_3:-4662541	Msa1271200:CDS
ACATTCATCATTCCAACACT+TGG	0.561131	8_3:-4663181	Msa1271200:CDS
ACTTTGGAGAGTGTGGTTAA+TGG	0.345363	8_3:-4662194	Msa1271200:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAGAATGAAGTCTATGTT+TGG	+	chr8_3:4662097-4662116	None:intergenic	30.0%
GGAATATCTTTGATATGGTA+AGG	+	chr8_3:4662445-4662464	None:intergenic	30.0%
GATCAGGAATATCTTTGATA+TGG	+	chr8_3:4662450-4662469	None:intergenic	30.0%
CAGAAATTCTCATTAGTTGA+TGG	-	chr8_3:4662523-4662542	Msa1271200:CDS	30.0%
TTAGACTCATTTCTGTTAAG+GGG	+	chr8_3:4662637-4662656	None:intergenic	30.0%
CCATTTGGTAAATACTCTAA+CGG	+	chr8_3:4662880-4662899	None:intergenic	30.0%
GTATTTACCAAATGGTTTCT+TGG	-	chr8_3:4662885-4662904	Msa1271200:CDS	30.0%
TTTCTTTCCAAGAAACCATT+TGG	+	chr8_3:4662895-4662914	None:intergenic	30.0%
TTGGAAAGAAACAGAGAAAA+TGG	-	chr8_3:4662904-4662923	Msa1271200:CDS	30.0%
AAGAAACAGAGAAAATGGAT+TGG	-	chr8_3:4662909-4662928	Msa1271200:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	4661908	4663323	4661908	ID=Msa1271200;Name=Msa1271200
chr8_3	mRNA	4661908	4663323	4661908	ID=Msa1271200-mRNA-1;Parent=Msa1271200;Name=Msa1271200-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|471
chr8_3	exon	4661908	4663323	4661908	ID=Msa1271200-mRNA-1:exon:1609;Parent=Msa1271200-mRNA-1
chr8_3	CDS	4661908	4663323	4661908	ID=Msa1271200-mRNA-1:cds;Parent=Msa1271200-mRNA-1

Gene Sequence

>Msa1271200

ATGCACATGATGAATAAAAAACCATGTATTGCTATGGTTCCTTCCCCTGGACTTAGCCATTTAATTCCACAAGTTGAATTTGCCAAACAATTACTTCAACAACACAATGAATTCATTGTCACATTCATCATTCCAACACTTGGTCCTCTTACTCCTTCAATGCAACAAGTCTTGAACAATCTTCCACCAAACATAGACTTCATTCTTCTTCCTCAAGTAAAAAACATTCAACAAAGCCATGATCCAGCAGCACAAATGAAACTCATTGTGAAACATTCTATTCCATTTCTACAACAAGAAGTCAAATCACTCATTTCCAAAACTAACCTTGTTTCTTTAATCTTTAGTATATTTTCAACAGATGCATATGAAGTTGCTAAACAGTTCAACTTGTCTTCTTATCTTTTCTATGCCTCAGGAGCTGTTCCAACATCGTTTTGTCTCACTCTTCCGATTCTCAACGACTCTATTTCCTCGGAAATAGAGTTCTTAGAGTCGGCTTATGAAACTGTGAATGTTCCAGGTAGTATAGTCCCTTACCATATCAAAGATATTCCTGATCCACTTCTATGTGAAAGATCAAGTTTAGAGTACAAATCATTTCTTGATGTATGTCAGAAATTCTCATTAGTTGATGGTCTTATCATAAACACTTTCAATGATTTGGAACCTGAGGTTATAAGGCTTCTTCAAGAAAAAAAGAAACCTTGTTTTTATCCAGTGGGACCCCTTAACAGAAATGAGTCTAATTGTGAAGATAACATTAATTCAATGTGTTTAAGGTGGTTGGAAAATCAACCACCAGGTTCTGTTCTTTATGTATGTTTTGGGAGTGGTGGAACAGTTTCTCATGAACAACTCAATGAGTTAGCATTTGGATTAGAATTGAGTGGCAAGAAATTCTTGTGGGTTGTGAGAGTTCCAAGTAAAGCTGTTAACTCTGCTTATTTTGTTGGAGCAAAAGATGATCCGTTAGAGTATTTACCAAATGGTTTCTTGGAAAGAAACAGAGAAAATGGATTGGTTGTTCCATCATGGGCACCTCAAGTTGAGATTCTTGGTCATGGTTCAATTGGTGGTTTTTTGAGTCATTGTGGTTGGGGTTCAACTTTGGAGAGTGTGGTTAATGGTGTTCCTATGATTGCGTGGCCTCTATTTGCAGAACAAAGGATGAATGCAAAATTATTGACAGATGAGCTCAAGGTGGCTGTGAGGCCGAAGATTGATGATGAAAGTGGGATAGTGAAAAGAGAAGAAGTTGCTAACTGTGTAAGGAGAATCATGGAAGGTGATGAAAGCTTGGAAATACAAAAGAGAATCAAGGAATTGAGTGTTGGTGCTGCTGTTGCAACAAGTGAAAATGGTTCATCTAAGAAGGCGCTCTCCTCTTTAGCATCGAGATTGCATAACTTTTGA

Protein sequence

>Msa1271200

MHMMNKKPCIAMVPSPGLSHLIPQVEFAKQLLQQHNEFIVTFIIPTLGPLTPSMQQVLNNLPPNIDFILLPQVKNIQQSHDPAAQMKLIVKHSIPFLQQEVKSLISKTNLVSLIFSIFSTDAYEVAKQFNLSSYLFYASGAVPTSFCLTLPILNDSISSEIEFLESAYETVNVPGSIVPYHIKDIPDPLLCERSSLEYKSFLDVCQKFSLVDGLIINTFNDLEPEVIRLLQEKKKPCFYPVGPLNRNESNCEDNINSMCLRWLENQPPGSVLYVCFGSGGTVSHEQLNELAFGLELSGKKFLWVVRVPSKAVNSAYFVGAKDDPLEYLPNGFLERNRENGLVVPSWAPQVEILGHGSIGGFLSHCGWGSTLESVVNGVPMIAWPLFAEQRMNAKLLTDELKVAVRPKIDDESGIVKREEVANCVRRIMEGDESLEIQKRIKELSVGAAVATSENGSSKKALSSLASRLHNF*