AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0978250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0978250 MtrunA17_Chr6g0457131 59.302 86 20 3 1 80 428 504 2.78e-22 90.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 26 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAATTATGATGAATTTA+TGG 0.129045 6_4:+13965126 Msa0978250:three_prime_UTR
TCAAATTCCATTATGATTCT+TGG 0.218604 6_4:+13963736 Msa0978250:CDS
CTTGTTTATTACAAATCTTC+TGG 0.227401 6_4:+13964800 Msa0978250:three_prime_UTR
ATCAACCCTGCTCAGTTCTT+TGG 0.273336 6_4:-13964850 None:intergenic
TTTCTCATTCTAGCTGTGTT+TGG 0.341726 6_4:+13964938 Msa0978250:three_prime_UTR
CTGGAGGTTGGCTGAAATCA+TGG 0.348465 6_4:+13964819 Msa0978250:three_prime_UTR
ATGCTATAGGAATGATATCT+TGG 0.360848 6_4:-13963714 None:intergenic
CATTTAGAATTGAACATAAA+CGG 0.379912 6_4:-13963782 None:intergenic
TAAAGACAACAAAACATAAT+AGG 0.384446 6_4:-13965075 None:intergenic
ATCTTGAAGTTTGCTGTGAC+AGG 0.430780 6_4:-13964740 None:intergenic
ACCTTGTCCAAGAATCATAA+TGG 0.436088 6_4:-13963743 None:intergenic
TTCTTGCCAAAGAACTGAGC+AGG 0.437750 6_4:+13964844 Msa0978250:three_prime_UTR
ATTACAAATCTTCTGGAGGT+TGG 0.463039 6_4:+13964807 Msa0978250:three_prime_UTR
AGATTATCGGTTCATGGGAC+TGG 0.465055 6_4:+13964664 Msa0978250:intron
TTGTAGGAAATCAATCATCT+TGG 0.469304 6_4:+13964054 Msa0978250:intron
TTGATATGAAGACATGATCT+TGG 0.479772 6_4:+13964868 Msa0978250:three_prime_UTR
TTGAACATAAACGGAAAGAA+AGG 0.481150 6_4:-13963773 None:intergenic
ATAATGGAATTTGATGCTAT+AGG 0.488771 6_4:-13963727 None:intergenic
ATGCATACATGTGTGGCTCC+AGG 0.497973 6_4:+13964093 Msa0978250:CDS
GATATCTTGGATCTTCTCAC+TGG 0.507926 6_4:-13963701 None:intergenic
CTATGAAAGACAATATATCC+TGG 0.525201 6_4:-13964111 None:intergenic
AGCTGTTGAATTCGACTGCA+CGG 0.548685 6_4:-13964695 None:intergenic
TCCATTATGATTCTTGGACA+AGG 0.564164 6_4:+13963742 Msa0978250:CDS
GTTTATTACAAATCTTCTGG+AGG 0.590293 6_4:+13964803 Msa0978250:three_prime_UTR
TCTTGCCAAAGAACTGAGCA+GGG 0.609369 6_4:+13964845 Msa0978250:three_prime_UTR
CGTTCTAATGCATACATGTG+TGG 0.675678 6_4:+13964086 Msa0978250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTATTTTATTAATTATCA+TGG + chr6_4:13964135-13964154 Msa0978250:intron 10.0%
!!! AAGTATTTTATTAATTATCA+TGG + chr6_4:13964135-13964154 Msa0978250:intron 10.0%
!!! ATTTCGTTTCTTTTTTATAT+TGG + chr6_4:13963503-13963522 Msa0978250:intron 15.0%
!! GAAATATAAATTCTATCAAA+AGG - chr6_4:13964525-13964544 None:intergenic 15.0%
!! GAAAAATCATTAAAATTATC+TGG - chr6_4:13965110-13965129 None:intergenic 15.0%
!! TTTCAATTATGATGAATTTA+TGG + chr6_4:13965126-13965145 Msa0978250:three_prime_UTR 15.0%
!!! ATTTCGTTTCTTTTTTATAT+TGG + chr6_4:13963503-13963522 Msa0978250:intron 15.0%
!! GAAATATAAATTCTATCAAA+AGG - chr6_4:13964525-13964544 None:intergenic 15.0%
!! GAAAAATCATTAAAATTATC+TGG - chr6_4:13965110-13965129 None:intergenic 15.0%
!! TTTCAATTATGATGAATTTA+TGG + chr6_4:13965126-13965145 Msa0978250:three_prime_UTR 15.0%
!! TATCTCTTGTAAATAAAATC+TGG + chr6_4:13963408-13963427 Msa0978250:intron 20.0%
!! TTCAACAATAGATTGAAAAT+TGG - chr6_4:13963578-13963597 None:intergenic 20.0%
!!! CATTTAGAATTGAACATAAA+CGG - chr6_4:13963785-13963804 None:intergenic 20.0%
!!! TAAATTTTTCATTATGCATC+TGG + chr6_4:13963833-13963852 Msa0978250:intron 20.0%
!! TGTTATTACATAACTTATGA+CGG - chr6_4:13964234-13964253 None:intergenic 20.0%
!! TCATAAGTTATGTAATAACA+TGG + chr6_4:13964234-13964253 Msa0978250:intron 20.0%
!!! TTTTTTTTGGTGTATATATG+TGG + chr6_4:13964384-13964403 Msa0978250:intron 20.0%
!! TATGTAAATATTCTGTTAGA+GGG - chr6_4:13964475-13964494 None:intergenic 20.0%
!! TTATGTAAATATTCTGTTAG+AGG - chr6_4:13964476-13964495 None:intergenic 20.0%
!!! TTACCGTTATTTTAGATTAT+CGG + chr6_4:13964651-13964670 Msa0978250:intron 20.0%
!! TAAAGACAACAAAACATAAT+AGG - chr6_4:13965078-13965097 None:intergenic 20.0%
!! TATCTCTTGTAAATAAAATC+TGG + chr6_4:13963408-13963427 Msa0978250:intron 20.0%
!! TTCAACAATAGATTGAAAAT+TGG - chr6_4:13963578-13963597 None:intergenic 20.0%
!!! CATTTAGAATTGAACATAAA+CGG - chr6_4:13963785-13963804 None:intergenic 20.0%
!!! TAAATTTTTCATTATGCATC+TGG + chr6_4:13963833-13963852 Msa0978250:intron 20.0%
!! TGTTATTACATAACTTATGA+CGG - chr6_4:13964234-13964253 None:intergenic 20.0%
!! TCATAAGTTATGTAATAACA+TGG + chr6_4:13964234-13964253 Msa0978250:intron 20.0%
!!! TTTTTTTTGGTGTATATATG+TGG + chr6_4:13964384-13964403 Msa0978250:intron 20.0%
!! TATGTAAATATTCTGTTAGA+GGG - chr6_4:13964475-13964494 None:intergenic 20.0%
!! TTATGTAAATATTCTGTTAG+AGG - chr6_4:13964476-13964495 None:intergenic 20.0%
!!! TTACCGTTATTTTAGATTAT+CGG + chr6_4:13964651-13964670 Msa0978250:intron 20.0%
!! TAAAGACAACAAAACATAAT+AGG - chr6_4:13965078-13965097 None:intergenic 20.0%
!! AAATATCTCCTTTTTGCTTT+TGG - chr6_4:13963251-13963270 None:intergenic 25.0%
! ACAATATGCTTAAAAATGCA+AGG - chr6_4:13963294-13963313 None:intergenic 25.0%
! TTGAAACAAAGGAATATCAA+GGG - chr6_4:13963348-13963367 None:intergenic 25.0%
! TTGTAAATAAAATCTGGATG+AGG + chr6_4:13963414-13963433 Msa0978250:intron 25.0%
! TACCTGAACATAATTAATGA+AGG + chr6_4:13963438-13963457 Msa0978250:intron 25.0%
!! ATAATGGAATTTGATGCTAT+AGG - chr6_4:13963730-13963749 None:intergenic 25.0%
! TCAAATTCCATTATGATTCT+TGG + chr6_4:13963736-13963755 Msa0978250:CDS 25.0%
! TATGTTCAATTCTAAATGCA+AGG + chr6_4:13963787-13963806 Msa0978250:intron 25.0%
! ATGTTCAATTCTAAATGCAA+GGG + chr6_4:13963788-13963807 Msa0978250:intron 25.0%
!! TGAGCAGTGAAAATTTTATA+CGG - chr6_4:13963902-13963921 None:intergenic 25.0%
!!! TTAATGTAAGCTTTGTTTGT+AGG + chr6_4:13964038-13964057 Msa0978250:intron 25.0%
! TTATTACATAACTTATGACG+GGG - chr6_4:13964232-13964251 None:intergenic 25.0%
! GTTATTACATAACTTATGAC+GGG - chr6_4:13964233-13964252 None:intergenic 25.0%
! ATGTTCTTAATTCTTGAGTT+AGG + chr6_4:13964262-13964281 Msa0978250:intron 25.0%
!!! CTTGTTTCGAAACTTTTTTT+TGG + chr6_4:13964371-13964390 Msa0978250:intron 25.0%
! AATATTTACATAATCATCGC+CGG + chr6_4:13964483-13964502 Msa0978250:intron 25.0%
! AACTAGAGTTGTAATGTATA+AGG - chr6_4:13964577-13964596 None:intergenic 25.0%
!! GAACCGATAATCTAAAATAA+CGG - chr6_4:13964657-13964676 None:intergenic 25.0%
!!! TATTTTAGATTATCGGTTCA+TGG + chr6_4:13964658-13964677 Msa0978250:intron 25.0%
!!! ATTTTAGATTATCGGTTCAT+GGG + chr6_4:13964659-13964678 Msa0978250:intron 25.0%
! CATGAATACAACAAAAATGT+TGG + chr6_4:13964765-13964784 Msa0978250:exon 25.0%
! CTTGTTTATTACAAATCTTC+TGG + chr6_4:13964800-13964819 Msa0978250:three_prime_UTR 25.0%
!! CTTTTATGAAAGATGTTTTG+TGG + chr6_4:13964963-13964982 Msa0978250:three_prime_UTR 25.0%
!! AAATATCTCCTTTTTGCTTT+TGG - chr6_4:13963251-13963270 None:intergenic 25.0%
! ACAATATGCTTAAAAATGCA+AGG - chr6_4:13963294-13963313 None:intergenic 25.0%
! TTGAAACAAAGGAATATCAA+GGG - chr6_4:13963348-13963367 None:intergenic 25.0%
! TTGTAAATAAAATCTGGATG+AGG + chr6_4:13963414-13963433 Msa0978250:intron 25.0%
! TACCTGAACATAATTAATGA+AGG + chr6_4:13963438-13963457 Msa0978250:intron 25.0%
!! ATAATGGAATTTGATGCTAT+AGG - chr6_4:13963730-13963749 None:intergenic 25.0%
! TCAAATTCCATTATGATTCT+TGG + chr6_4:13963736-13963755 Msa0978250:CDS 25.0%
! TATGTTCAATTCTAAATGCA+AGG + chr6_4:13963787-13963806 Msa0978250:intron 25.0%
! ATGTTCAATTCTAAATGCAA+GGG + chr6_4:13963788-13963807 Msa0978250:intron 25.0%
!! TGAGCAGTGAAAATTTTATA+CGG - chr6_4:13963902-13963921 None:intergenic 25.0%
!!! TTAATGTAAGCTTTGTTTGT+AGG + chr6_4:13964038-13964057 Msa0978250:intron 25.0%
! TTATTACATAACTTATGACG+GGG - chr6_4:13964232-13964251 None:intergenic 25.0%
! GTTATTACATAACTTATGAC+GGG - chr6_4:13964233-13964252 None:intergenic 25.0%
! ATGTTCTTAATTCTTGAGTT+AGG + chr6_4:13964262-13964281 Msa0978250:intron 25.0%
!!! CTTGTTTCGAAACTTTTTTT+TGG + chr6_4:13964371-13964390 Msa0978250:intron 25.0%
! AATATTTACATAATCATCGC+CGG + chr6_4:13964483-13964502 Msa0978250:intron 25.0%
! AACTAGAGTTGTAATGTATA+AGG - chr6_4:13964577-13964596 None:intergenic 25.0%
!! GAACCGATAATCTAAAATAA+CGG - chr6_4:13964657-13964676 None:intergenic 25.0%
!!! TATTTTAGATTATCGGTTCA+TGG + chr6_4:13964658-13964677 Msa0978250:intron 25.0%
!!! ATTTTAGATTATCGGTTCAT+GGG + chr6_4:13964659-13964678 Msa0978250:intron 25.0%
! CATGAATACAACAAAAATGT+TGG + chr6_4:13964765-13964784 Msa0978250:exon 25.0%
! CTTGTTTATTACAAATCTTC+TGG + chr6_4:13964800-13964819 Msa0978250:three_prime_UTR 25.0%
!! CTTTTATGAAAGATGTTTTG+TGG + chr6_4:13964963-13964982 Msa0978250:three_prime_UTR 25.0%
AGCAAAAAGGAGATATTTGA+AGG + chr6_4:13963253-13963272 Msa0978250:intron 30.0%
GTTGAAACAAAGGAATATCA+AGG - chr6_4:13963349-13963368 None:intergenic 30.0%
CAAGTACAAAGTTGAAACAA+AGG - chr6_4:13963359-13963378 None:intergenic 30.0%
! CTTTGTTTCAACTTTGTACT+TGG + chr6_4:13963357-13963376 Msa0978250:intron 30.0%
TGCCTTCATTAATTATGTTC+AGG - chr6_4:13963443-13963462 None:intergenic 30.0%
TCAAAGCTATTATTAGACCT+AGG + chr6_4:13963530-13963549 Msa0978250:intron 30.0%
ATGCTATAGGAATGATATCT+TGG - chr6_4:13963717-13963736 None:intergenic 30.0%
TTGAACATAAACGGAAAGAA+AGG - chr6_4:13963776-13963795 None:intergenic 30.0%
! TAAAATTTTCACTGCTCAAC+AGG + chr6_4:13963904-13963923 Msa0978250:intron 30.0%
TTGTAGGAAATCAATCATCT+TGG + chr6_4:13964054-13964073 Msa0978250:intron 30.0%
CTATGAAAGACAATATATCC+TGG - chr6_4:13964114-13964133 None:intergenic 30.0%
!! GAAAGGTATGTAAACTTTTC+TGG + chr6_4:13964333-13964352 Msa0978250:intron 30.0%
AGGTGAAACAAATAGTTATC+CGG - chr6_4:13964505-13964524 None:intergenic 30.0%
GTTTATTACAAATCTTCTGG+AGG + chr6_4:13964803-13964822 Msa0978250:three_prime_UTR 30.0%
TTGATATGAAGACATGATCT+TGG + chr6_4:13964868-13964887 Msa0978250:three_prime_UTR 30.0%
AGCAAAAAGGAGATATTTGA+AGG + chr6_4:13963253-13963272 Msa0978250:intron 30.0%
GTTGAAACAAAGGAATATCA+AGG - chr6_4:13963349-13963368 None:intergenic 30.0%
CAAGTACAAAGTTGAAACAA+AGG - chr6_4:13963359-13963378 None:intergenic 30.0%
! CTTTGTTTCAACTTTGTACT+TGG + chr6_4:13963357-13963376 Msa0978250:intron 30.0%
TGCCTTCATTAATTATGTTC+AGG - chr6_4:13963443-13963462 None:intergenic 30.0%
TCAAAGCTATTATTAGACCT+AGG + chr6_4:13963530-13963549 Msa0978250:intron 30.0%
ATGCTATAGGAATGATATCT+TGG - chr6_4:13963717-13963736 None:intergenic 30.0%
TTGAACATAAACGGAAAGAA+AGG - chr6_4:13963776-13963795 None:intergenic 30.0%
! TAAAATTTTCACTGCTCAAC+AGG + chr6_4:13963904-13963923 Msa0978250:intron 30.0%
TTGTAGGAAATCAATCATCT+TGG + chr6_4:13964054-13964073 Msa0978250:intron 30.0%
CTATGAAAGACAATATATCC+TGG - chr6_4:13964114-13964133 None:intergenic 30.0%
!! GAAAGGTATGTAAACTTTTC+TGG + chr6_4:13964333-13964352 Msa0978250:intron 30.0%
AGGTGAAACAAATAGTTATC+CGG - chr6_4:13964505-13964524 None:intergenic 30.0%
GTTTATTACAAATCTTCTGG+AGG + chr6_4:13964803-13964822 Msa0978250:three_prime_UTR 30.0%
TTGATATGAAGACATGATCT+TGG + chr6_4:13964868-13964887 Msa0978250:three_prime_UTR 30.0%
TTCAGTGACCAAAAGCAAAA+AGG + chr6_4:13963240-13963259 Msa0978250:intron 35.0%
TATTCAGTCAACACTTTCCT+AGG - chr6_4:13963550-13963569 None:intergenic 35.0%
ATTCTCTTCCAAAATGCCTT+TGG + chr6_4:13963601-13963620 Msa0978250:intron 35.0%
TCCATTATGATTCTTGGACA+AGG + chr6_4:13963742-13963761 Msa0978250:CDS 35.0%
ACCTTGTCCAAGAATCATAA+TGG - chr6_4:13963746-13963765 None:intergenic 35.0%
TGAAAAATCGAAGCAACTTC+AGG - chr6_4:13963953-13963972 None:intergenic 35.0%
! AGTTGCTTCGATTTTTCATC+TGG + chr6_4:13963955-13963974 Msa0978250:CDS 35.0%
!!! GTAAACTTTTCTGGTTACCT+CGG + chr6_4:13964342-13964361 Msa0978250:intron 35.0%
! ATTACAAATCTTCTGGAGGT+TGG + chr6_4:13964807-13964826 Msa0978250:three_prime_UTR 35.0%
! AATTTTGCTTCTCTGCTTGT+TGG + chr6_4:13964892-13964911 Msa0978250:three_prime_UTR 35.0%
! TTTCTCATTCTAGCTGTGTT+TGG + chr6_4:13964938-13964957 Msa0978250:three_prime_UTR 35.0%
! TGAAAGATGTTTTGTGGTGT+TGG + chr6_4:13964969-13964988 Msa0978250:three_prime_UTR 35.0%
TTCAGTGACCAAAAGCAAAA+AGG + chr6_4:13963240-13963259 Msa0978250:intron 35.0%
TATTCAGTCAACACTTTCCT+AGG - chr6_4:13963550-13963569 None:intergenic 35.0%
ATTCTCTTCCAAAATGCCTT+TGG + chr6_4:13963601-13963620 Msa0978250:intron 35.0%
TCCATTATGATTCTTGGACA+AGG + chr6_4:13963742-13963761 Msa0978250:CDS 35.0%
ACCTTGTCCAAGAATCATAA+TGG - chr6_4:13963746-13963765 None:intergenic 35.0%
TGAAAAATCGAAGCAACTTC+AGG - chr6_4:13963953-13963972 None:intergenic 35.0%
! AGTTGCTTCGATTTTTCATC+TGG + chr6_4:13963955-13963974 Msa0978250:CDS 35.0%
!!! GTAAACTTTTCTGGTTACCT+CGG + chr6_4:13964342-13964361 Msa0978250:intron 35.0%
! ATTACAAATCTTCTGGAGGT+TGG + chr6_4:13964807-13964826 Msa0978250:three_prime_UTR 35.0%
! AATTTTGCTTCTCTGCTTGT+TGG + chr6_4:13964892-13964911 Msa0978250:three_prime_UTR 35.0%
! TTTCTCATTCTAGCTGTGTT+TGG + chr6_4:13964938-13964957 Msa0978250:three_prime_UTR 35.0%
! TGAAAGATGTTTTGTGGTGT+TGG + chr6_4:13964969-13964988 Msa0978250:three_prime_UTR 35.0%
GATATCTTGGATCTTCTCAC+TGG - chr6_4:13963704-13963723 None:intergenic 40.0%
CGTTCTAATGCATACATGTG+TGG + chr6_4:13964086-13964105 Msa0978250:CDS 40.0%
! ACATAACTTATGACGGGGAA+AGG - chr6_4:13964227-13964246 None:intergenic 40.0%
AATTCTTGAGTTAGGTGCTC+TGG + chr6_4:13964270-13964289 Msa0978250:intron 40.0%
ATAGCGCATCCTTAAGATAC+TGG + chr6_4:13964303-13964322 Msa0978250:intron 40.0%
TCGAGATCACCAGTATCTTA+AGG - chr6_4:13964315-13964334 None:intergenic 40.0%
AAGATACTGGTGATCTCGAA+AGG + chr6_4:13964316-13964335 Msa0978250:intron 40.0%
TTCGAAACAAGTAAGAACCG+AGG - chr6_4:13964362-13964381 None:intergenic 40.0%
GGTGTATATATGTGGCTGTT+AGG + chr6_4:13964392-13964411 Msa0978250:intron 40.0%
!! ATCTTGAAGTTTGCTGTGAC+AGG - chr6_4:13964743-13964762 None:intergenic 40.0%
!! AAGATGTTTTGTGGTGTTGG+AGG + chr6_4:13964972-13964991 Msa0978250:three_prime_UTR 40.0%
GATATCTTGGATCTTCTCAC+TGG - chr6_4:13963704-13963723 None:intergenic 40.0%
CGTTCTAATGCATACATGTG+TGG + chr6_4:13964086-13964105 Msa0978250:CDS 40.0%
! ACATAACTTATGACGGGGAA+AGG - chr6_4:13964227-13964246 None:intergenic 40.0%
AATTCTTGAGTTAGGTGCTC+TGG + chr6_4:13964270-13964289 Msa0978250:intron 40.0%
ATAGCGCATCCTTAAGATAC+TGG + chr6_4:13964303-13964322 Msa0978250:intron 40.0%
TCGAGATCACCAGTATCTTA+AGG - chr6_4:13964315-13964334 None:intergenic 40.0%
AAGATACTGGTGATCTCGAA+AGG + chr6_4:13964316-13964335 Msa0978250:intron 40.0%
TTCGAAACAAGTAAGAACCG+AGG - chr6_4:13964362-13964381 None:intergenic 40.0%
GGTGTATATATGTGGCTGTT+AGG + chr6_4:13964392-13964411 Msa0978250:intron 40.0%
!! ATCTTGAAGTTTGCTGTGAC+AGG - chr6_4:13964743-13964762 None:intergenic 40.0%
!! AAGATGTTTTGTGGTGTTGG+AGG + chr6_4:13964972-13964991 Msa0978250:three_prime_UTR 40.0%
!!! GAAGCTCTCCAAAGGCATTT+TGG - chr6_4:13963612-13963631 None:intergenic 45.0%
ATGAGACAGAAGCTCTCCAA+AGG - chr6_4:13963620-13963639 None:intergenic 45.0%
CTTAAGGATGCGCTATAGTG+AGG - chr6_4:13964299-13964318 None:intergenic 45.0%
TATGTGGCTGTTAGGAGTCT+AGG + chr6_4:13964400-13964419 Msa0978250:intron 45.0%
!! AGATTATCGGTTCATGGGAC+TGG + chr6_4:13964664-13964683 Msa0978250:intron 45.0%
AGCTGTTGAATTCGACTGCA+CGG - chr6_4:13964698-13964717 None:intergenic 45.0%
!! TTTGCTGTGACAGGTTTTGC+AGG - chr6_4:13964734-13964753 None:intergenic 45.0%
TTCTTGCCAAAGAACTGAGC+AGG + chr6_4:13964844-13964863 Msa0978250:three_prime_UTR 45.0%
TCTTGCCAAAGAACTGAGCA+GGG + chr6_4:13964845-13964864 Msa0978250:three_prime_UTR 45.0%
ATCAACCCTGCTCAGTTCTT+TGG - chr6_4:13964853-13964872 None:intergenic 45.0%
!!! GAAGCTCTCCAAAGGCATTT+TGG - chr6_4:13963612-13963631 None:intergenic 45.0%
ATGAGACAGAAGCTCTCCAA+AGG - chr6_4:13963620-13963639 None:intergenic 45.0%
CTTAAGGATGCGCTATAGTG+AGG - chr6_4:13964299-13964318 None:intergenic 45.0%
TATGTGGCTGTTAGGAGTCT+AGG + chr6_4:13964400-13964419 Msa0978250:intron 45.0%
!! AGATTATCGGTTCATGGGAC+TGG + chr6_4:13964664-13964683 Msa0978250:intron 45.0%
AGCTGTTGAATTCGACTGCA+CGG - chr6_4:13964698-13964717 None:intergenic 45.0%
!! TTTGCTGTGACAGGTTTTGC+AGG - chr6_4:13964734-13964753 None:intergenic 45.0%
TTCTTGCCAAAGAACTGAGC+AGG + chr6_4:13964844-13964863 Msa0978250:three_prime_UTR 45.0%
TCTTGCCAAAGAACTGAGCA+GGG + chr6_4:13964845-13964864 Msa0978250:three_prime_UTR 45.0%
ATCAACCCTGCTCAGTTCTT+TGG - chr6_4:13964853-13964872 None:intergenic 45.0%
ATGCATACATGTGTGGCTCC+AGG + chr6_4:13964093-13964112 Msa0978250:CDS 50.0%
! GACAGGTTTTGCAGGACAGA+TGG - chr6_4:13964726-13964745 None:intergenic 50.0%
! CTGGAGGTTGGCTGAAATCA+TGG + chr6_4:13964819-13964838 Msa0978250:three_prime_UTR 50.0%
ATGCATACATGTGTGGCTCC+AGG + chr6_4:13964093-13964112 Msa0978250:CDS 50.0%
! GACAGGTTTTGCAGGACAGA+TGG - chr6_4:13964726-13964745 None:intergenic 50.0%
! CTGGAGGTTGGCTGAAATCA+TGG + chr6_4:13964819-13964838 Msa0978250:three_prime_UTR 50.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 13963195 13965156 13963195 ID=Msa0978250;Name=Msa0978250
chr6_4 mRNA 13963195 13965156 13963195 ID=Msa0978250-mRNA-1;Parent=Msa0978250;Name=Msa0978250-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|0.25|0.6|0.8|0|0|5|387|113
chr6_4 exon 13963195 13963201 13963195 ID=Msa0978250-mRNA-1:exon:4603;Parent=Msa0978250-mRNA-1
chr6_4 exon 13963683 13963789 13963683 ID=Msa0978250-mRNA-1:exon:4604;Parent=Msa0978250-mRNA-1
chr6_4 exon 13963926 13963976 13963926 ID=Msa0978250-mRNA-1:exon:4605;Parent=Msa0978250-mRNA-1
chr6_4 exon 13964060 13964132 13964060 ID=Msa0978250-mRNA-1:exon:4606;Parent=Msa0978250-mRNA-1
chr6_4 exon 13964666 13965156 13964666 ID=Msa0978250-mRNA-1:exon:4607;Parent=Msa0978250-mRNA-1
chr6_4 CDS 13963195 13963201 13963195 ID=Msa0978250-mRNA-1:cds;Parent=Msa0978250-mRNA-1
chr6_4 CDS 13963683 13963789 13963683 ID=Msa0978250-mRNA-1:cds;Parent=Msa0978250-mRNA-1
chr6_4 CDS 13963926 13963976 13963926 ID=Msa0978250-mRNA-1:cds;Parent=Msa0978250-mRNA-1
chr6_4 CDS 13964060 13964132 13964060 ID=Msa0978250-mRNA-1:cds;Parent=Msa0978250-mRNA-1
chr6_4 CDS 13964666 13964769 13964666 ID=Msa0978250-mRNA-1:cds;Parent=Msa0978250-mRNA-1
chr6_4 three_prime_UTR 13964770 13965156 13964770 ID=Msa0978250-mRNA-1:three_prime_utr;Parent=Msa0978250-mRNA-1
Gene Sequence

>Msa0978250

ATGGACATTACTCAAGTTCTTTTCTCCAGTGAGAAGATCCAAGATATCATTCCTATAGCATCAAATTCCATTATGATTCTTGGACAAGGTAATTTTTCCTTTCTTTCCGTTTATGAAGTTTATATGCATATGCAATTTCCTGAAGTTGCTTCGATTTTTCATCTGGAAATCAATCATCTTGGCTTGCTTGACGTTCTAATGCATACATGTGTGGCTCCAGGATATATTGTCTTTCATAATTATCGGTTCATGGGACTGGTTAGATATCCGTGCAGTCGAATTCAACAGCTCAAAGCCATCTGTCCTGCAAAACCTGTCACAGCAAACTTCAAGATAACATGA
Protein sequence

>Msa0978250

MDITQVLFSSEKIQDIIPIASNSIMILGQGNFSFLSVYEVYMHMQFPEVASIFHLEINHLGLLDVLMHTCVAPGYIVFHNYRFMGLVRYPCSRIQQLKAICPAKPVTANFKIT*