AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1449820


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1449820 MtrunA17_Chr8g0387141 98.077 52 1 0 18 69 545 596 4.21e-31 113
Msa1449820 MtrunA17_Chr8g0387181 78.846 52 11 0 18 69 577 628 2.49e-25 96.7
Msa1449820 MtrunA17_Chr8g0387161 71.154 52 15 0 18 69 246 297 3.46e-21 83.6
Msa1449820 MtrunA17_Chr1g0186281 76.087 46 11 0 18 63 534 579 2.84e-18 76.6
Msa1449820 MtrunA17_Chr1g0186261 76.087 46 11 0 18 63 537 582 4.11e-18 76.3
Msa1449820 MtrunA17_Chr5g0420461 75.610 41 10 0 29 69 157 197 1.93e-17 72.0
Msa1449820 MtrunA17_Chr1g0186191 73.913 46 12 0 18 63 544 589 4.27e-17 73.6
Msa1449820 MtrunA17_Chr6g0479271 78.378 37 8 0 33 69 292 328 1.00e-15 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 12 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGATGACGAAGTGGAATT+TGG 0.312152 tig0032705:-38442 Msa1449820:CDS
AGCTATCAGGCATCCATCTA+TGG 0.376521 tig0032705:-38392 Msa1449820:CDS
GCATCCATCTATGGAATTCA+TGG 0.384589 tig0032705:-38383 Msa1449820:CDS
CACTCCATGAATTCCATAGA+TGG 0.401034 tig0032705:+38379 None:intergenic
GTTTCGGAAGGAAAGCTATC+AGG 0.412531 tig0032705:-38405 Msa1449820:CDS
TTGCCTTCCTTCGAAAGCAT+TGG 0.437415 tig0032705:+38555 None:intergenic
TCATCACTGTCAACAGCATT+CGG 0.478391 tig0032705:+38460 None:intergenic
CATCCAATGCTTTCGAAGGA+AGG 0.521611 tig0032705:-38558 None:intergenic
AATCCATCCAATGCTTTCGA+AGG 0.574078 tig0032705:-38562 None:intergenic
AAGGAAGGCAAGAAAAGACT+TGG 0.584242 tig0032705:-38543 Msa1449820:CDS
AGGCAAGAAAAGACTTGGTG+AGG 0.649236 tig0032705:-38538 Msa1449820:CDS
TGACAGTGATGATGACGAAG+TGG 0.678639 tig0032705:-38449 Msa1449820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAATTTGGATCTTTTGATGA+TGG - tig0032705:38470-38489 Msa1449820:CDS 30.0%
!!! GGATCTTTTGATGATGGTTT+CGG - tig0032705:38476-38495 Msa1449820:CDS 35.0%
AAGGAAGGCAAGAAAAGACT+TGG - tig0032705:38354-38373 Msa1449820:CDS 40.0%
TCATCACTGTCAACAGCATT+CGG + tig0032705:38440-38459 None:intergenic 40.0%
! GATGATGACGAAGTGGAATT+TGG - tig0032705:38455-38474 Msa1449820:CDS 40.0%
!!! CTTTTGATGATGGTTTCGGA+AGG - tig0032705:38480-38499 Msa1449820:CDS 40.0%
GCATCCATCTATGGAATTCA+TGG - tig0032705:38514-38533 Msa1449820:intron 40.0%
CACTCCATGAATTCCATAGA+TGG + tig0032705:38521-38540 None:intergenic 40.0%
!! AGGCAAGAAAAGACTTGGTG+AGG - tig0032705:38359-38378 Msa1449820:CDS 45.0%
! TGACAGTGATGATGACGAAG+TGG - tig0032705:38448-38467 Msa1449820:CDS 45.0%
GTTTCGGAAGGAAAGCTATC+AGG - tig0032705:38492-38511 Msa1449820:intron 45.0%
AGCTATCAGGCATCCATCTA+TGG - tig0032705:38505-38524 Msa1449820:intron 45.0%
Chromosome Type Strat End Strand Name
tig0032705 gene 38345 38574 38345 ID=Msa1449820;Name=Msa1449820
tig0032705 mRNA 38345 38574 38345 ID=Msa1449820-mRNA-1;Parent=Msa1449820;Name=Msa1449820-mRNA-1;_AED=0.49;_eAED=0.61;_QI=0|0|0|1|0|0.5|2|0|69
tig0032705 exon 38525 38574 38525 ID=Msa1449820-mRNA-1:exon:17147;Parent=Msa1449820-mRNA-1
tig0032705 exon 38345 38504 38345 ID=Msa1449820-mRNA-1:exon:17146;Parent=Msa1449820-mRNA-1
tig0032705 CDS 38525 38574 38525 ID=Msa1449820-mRNA-1:cds;Parent=Msa1449820-mRNA-1
tig0032705 CDS 38345 38504 38345 ID=Msa1449820-mRNA-1:cds;Parent=Msa1449820-mRNA-1
Gene Sequence

>Msa1449820

ATGCTTTCGAAGGAAGGCAAGAAAAGACTTGGTGAGGTATTACTTTCAATAAGCTATCAGAATCGAGTGACTCCGAATGCTGTTGACAGTGATGATGACGAAGTGGAATTTGGATCTTTTGATGATGGTTTCGGAAGGAAAGCTATCAGGCATCCATCTATGGAATTCATGGAGTGTACAGAGAACAAGCAATGCTTTGATTTTGAGTAG

Protein sequence

>Msa1449820

MLSKEGKKRLGEVLLSISYQNRVTPNAVDSDDDEVEFGSFDDGFGRKAIRHPSMEFMECTENKQCFDFE*