AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0142660


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0142660 MtrunA17_Chr2g0286621 92.248 129 9 1 1 128 1 129 3.38e-81 234
Msa0142660 MtrunA17_Chr2g0286631 80.435 138 17 3 1 128 1 138 1.77e-57 174
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAATGGTTAATGTTAATT+TGG 0.155770 1_4:+1604524 Msa0142660:CDS
GATTCTGAAGGTGACCTTAT+TGG 0.270756 1_4:+1604270 Msa0142660:CDS
AAGGAAGATGAGAATGATAA+AGG 0.271877 1_4:+1604396 Msa0142660:CDS
TCATAAAGAAATGAATGATA+TGG 0.276443 1_4:+1604206 None:intergenic
ATGTTGATGATAAACTATTT+TGG 0.278652 1_4:+1604550 Msa0142660:CDS
TACCCTGCTGCTAATTACTT+TGG 0.359178 1_4:+1604306 Msa0142660:CDS
TTCATCTTTGTTGCTTATTG+AGG 0.383420 1_4:+1604239 Msa0142660:CDS
ATAGAGTAAAGAAGCCAATA+AGG 0.396823 1_4:-1604284 None:intergenic
ACCCTGCTGCTAATTACTTT+GGG 0.397321 1_4:+1604307 Msa0142660:CDS
TGGGAGATATGTATGGAAGT+AGG 0.434821 1_4:+1604570 Msa0142660:CDS
GGGAGATATGTATGGAAGTA+GGG 0.453186 1_4:+1604571 Msa0142660:CDS
TGGTATGTGTAGCTTAGTTA+AGG 0.466313 1_4:+1604377 Msa0142660:CDS
AGGAAGATGAGAATGATAAA+GGG 0.474639 1_4:+1604397 Msa0142660:CDS
GATGAGAATGATAAAGGGTC+AGG 0.478591 1_4:+1604402 Msa0142660:CDS
TGCTAATTACTTTGGGCACA+AGG 0.479009 1_4:+1604314 Msa0142660:CDS
TGCCCAAAGTAATTAGCAGC+AGG 0.488758 1_4:-1604309 None:intergenic
TCCTCATCATCAACAAGTAA+AGG 0.503410 1_4:-1604495 None:intergenic
ACCTTTACTTGTTGATGATG+AGG 0.506285 1_4:+1604494 Msa0142660:CDS
ACCATCATTGTCACCCTCAC+AGG 0.527345 1_4:-1604358 None:intergenic
AGAAGAGCATTCTATTTCCA+TGG 0.535708 1_4:-1604469 None:intergenic
TGATGAGGAGGAAATAAGAA+TGG 0.564678 1_4:+1604509 Msa0142660:CDS
TTTACTTGTTGATGATGAGG+AGG 0.584354 1_4:+1604497 Msa0142660:CDS
GTGGTTATCAGATGCAACCA+TGG 0.627194 1_4:+1604452 Msa0142660:CDS
ACCTGTGAGGGTGACAATGA+TGG 0.639274 1_4:+1604357 Msa0142660:CDS
GAGGACAGTGCAGATTCTGA+AGG 0.642136 1_4:+1604258 Msa0142660:CDS
GCCCAAAGTAATTAGCAGCA+GGG 0.657577 1_4:-1604308 None:intergenic
GCTGAGTCATGTACCTGTGA+GGG 0.671916 1_4:+1604345 Msa0142660:CDS
TGCTGAGTCATGTACCTGTG+AGG 0.698564 1_4:+1604344 Msa0142660:CDS
GGTTGCATCTGATAACCACA+TGG 0.727761 1_4:-1604448 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATGGTTAATGTTAATT+TGG + chr1_4:1604524-1604543 Msa0142660:CDS 20.0%
!!! ATGTTGATGATAAACTATTT+TGG + chr1_4:1604550-1604569 Msa0142660:CDS 20.0%
!!! TGTTGATGATAAACTATTTT+GGG + chr1_4:1604551-1604570 Msa0142660:CDS 20.0%
! TTCATCTTTGTTGCTTATTG+AGG + chr1_4:1604239-1604258 Msa0142660:CDS 30.0%
ATAGAGTAAAGAAGCCAATA+AGG - chr1_4:1604287-1604306 None:intergenic 30.0%
AAGGAAGATGAGAATGATAA+AGG + chr1_4:1604396-1604415 Msa0142660:CDS 30.0%
AGGAAGATGAGAATGATAAA+GGG + chr1_4:1604397-1604416 Msa0142660:CDS 30.0%
!! ACTATTTTGGGAGATATGTA+TGG + chr1_4:1604563-1604582 Msa0142660:CDS 30.0%
TGGTATGTGTAGCTTAGTTA+AGG + chr1_4:1604377-1604396 Msa0142660:CDS 35.0%
!! AGAAGAGCATTCTATTTCCA+TGG - chr1_4:1604472-1604491 None:intergenic 35.0%
!! ACCTTTACTTGTTGATGATG+AGG + chr1_4:1604494-1604513 Msa0142660:CDS 35.0%
TCCTCATCATCAACAAGTAA+AGG - chr1_4:1604498-1604517 None:intergenic 35.0%
!! TTTACTTGTTGATGATGAGG+AGG + chr1_4:1604497-1604516 Msa0142660:CDS 35.0%
TGATGAGGAGGAAATAAGAA+TGG + chr1_4:1604509-1604528 Msa0142660:CDS 35.0%
! GATTCTGAAGGTGACCTTAT+TGG + chr1_4:1604270-1604289 Msa0142660:CDS 40.0%
TACCCTGCTGCTAATTACTT+TGG + chr1_4:1604306-1604325 Msa0142660:CDS 40.0%
ACCCTGCTGCTAATTACTTT+GGG + chr1_4:1604307-1604326 Msa0142660:CDS 40.0%
TGCTAATTACTTTGGGCACA+AGG + chr1_4:1604314-1604333 Msa0142660:CDS 40.0%
GATGAGAATGATAAAGGGTC+AGG + chr1_4:1604402-1604421 Msa0142660:CDS 40.0%
TGGGAGATATGTATGGAAGT+AGG + chr1_4:1604570-1604589 Msa0142660:CDS 40.0%
GGGAGATATGTATGGAAGTA+GGG + chr1_4:1604571-1604590 Msa0142660:CDS 40.0%
GCCCAAAGTAATTAGCAGCA+GGG - chr1_4:1604311-1604330 None:intergenic 45.0%
TGCCCAAAGTAATTAGCAGC+AGG - chr1_4:1604312-1604331 None:intergenic 45.0%
!!! GCTGTTTTAGCTCTACCATG+TGG + chr1_4:1604433-1604452 Msa0142660:CDS 45.0%
GGTTGCATCTGATAACCACA+TGG - chr1_4:1604451-1604470 None:intergenic 45.0%
GTGGTTATCAGATGCAACCA+TGG + chr1_4:1604452-1604471 Msa0142660:CDS 45.0%
GAGGACAGTGCAGATTCTGA+AGG + chr1_4:1604258-1604277 Msa0142660:CDS 50.0%
TGCTGAGTCATGTACCTGTG+AGG + chr1_4:1604344-1604363 Msa0142660:CDS 50.0%
GCTGAGTCATGTACCTGTGA+GGG + chr1_4:1604345-1604364 Msa0142660:CDS 50.0%
ACCTGTGAGGGTGACAATGA+TGG + chr1_4:1604357-1604376 Msa0142660:CDS 50.0%
ACCATCATTGTCACCCTCAC+AGG - chr1_4:1604361-1604380 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 1604216 1604602 1604216 ID=Msa0142660;Name=Msa0142660
chr1_4 mRNA 1604216 1604602 1604216 ID=Msa0142660-mRNA-1;Parent=Msa0142660;Name=Msa0142660-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|128
chr1_4 exon 1604216 1604602 1604216 ID=Msa0142660-mRNA-1:exon:600;Parent=Msa0142660-mRNA-1
chr1_4 CDS 1604216 1604602 1604216 ID=Msa0142660-mRNA-1:cds;Parent=Msa0142660-mRNA-1
Gene Sequence

>Msa0142660

ATGAATGATATGGATGTATCATGTTCATCTTTGTTGCTTATTGAGGACAGTGCAGATTCTGAAGGTGACCTTATTGGCTTCTTTACTCTATACCCTGCTGCTAATTACTTTGGGCACAAGGAAGATGATGCTGAGTCATGTACCTGTGAGGGTGACAATGATGGTATGTGTAGCTTAGTTAAGGAAGATGAGAATGATAAAGGGTCAGGTGAATTTTGCTGTTTTAGCTCTACCATGTGGTTATCAGATGCAACCATGGAAATAGAATGCTCTTCTTCACCTTTACTTGTTGATGATGAGGAGGAAATAAGAATGGTTAATGTTAATTTGGATGATGTTGATGATAAACTATTTTGGGAGATATGTATGGAAGTAGGGTACCCTTGA

Protein sequence

>Msa0142660

MNDMDVSCSSLLLIEDSADSEGDLIGFFTLYPAANYFGHKEDDAESCTCEGDNDGMCSLVKEDENDKGSGEFCCFSSTMWLSDATMEIECSSSPLLVDDEEEIRMVNVNLDDVDDKLFWEICMEVGYP*