AlfalfaGEDB | MS.gene30542

M. sativa cultivar XinJiangDaYe / MS.gene30542

KEGG: K14686
GO: GO:0000041 GO:0003674 GO:0005215 GO:0005375 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0016021 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0034220 GO:0035434 GO:0046873 GO:0046915 GO:0051179 GO:0051234 GO:0055085 GO:0098655 GO:0098660 GO:0098662 GO:0110165
PFAM: PF04145.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30542.t1	XP_013456169.1	84.2	165	23	1	1	162	1	165	9.30E-69	269.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30542.t1	Q8GWP3	50.3	147	67	3	12	156	1	143	1.9e-30	133.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30542.t1	A0A072UWE8	84.2	165	23	1	1	162	1	165	6.7e-69	269.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene30542	0.872955	2.14E-67	-1.69E-46
MS.gene049173	MS.gene30542	0.814955	1.18E-51	-1.69E-46
MS.gene049217	MS.gene30542	0.866853	2.10E-65	-1.69E-46
MS.gene049222	MS.gene30542	0.873841	1.08E-67	-1.69E-46
MS.gene049232	MS.gene30542	0.879046	1.71E-69	-1.69E-46
MS.gene049285	MS.gene30542	0.9005	6.89E-78	-1.69E-46
MS.gene049320	MS.gene30542	0.912642	1.54E-83	-1.69E-46
MS.gene04939	MS.gene30542	0.816426	5.56E-52	-1.69E-46
MS.gene049609	MS.gene30542	0.837515	4.97E-57	-1.69E-46
MS.gene049611	MS.gene30542	-0.820694	5.99E-53	-1.69E-46
MS.gene049627	MS.gene30542	0.805872	1.08E-49	-1.69E-46
MS.gene049633	MS.gene30542	0.848865	4.67E-60	-1.69E-46
MS.gene049805	MS.gene30542	0.828639	8.04E-55	-1.69E-46
MS.gene05010	MS.gene30542	0.877688	5.14E-69	-1.69E-46
MS.gene050163	MS.gene30542	0.881366	2.55E-70	-1.69E-46
MS.gene050165	MS.gene30542	0.87666	1.17E-68	-1.69E-46
MS.gene050170	MS.gene30542	0.850339	1.81E-60	-1.69E-46
MS.gene050216	MS.gene30542	0.833235	5.99E-56	-1.69E-46
MS.gene050323	MS.gene30542	0.880845	3.92E-70	-1.69E-46
MS.gene050333	MS.gene30542	0.849715	2.71E-60	-1.69E-46
MS.gene050381	MS.gene30542	-0.820687	6.01E-53	-1.69E-46
MS.gene050444	MS.gene30542	0.849542	3.03E-60	-1.69E-46
MS.gene050496	MS.gene30542	0.856892	2.37E-62	-1.69E-46
MS.gene050504	MS.gene30542	0.919556	3.88E-87	-1.69E-46
MS.gene050518	MS.gene30542	0.883497	4.27E-71	-1.69E-46
MS.gene050577	MS.gene30542	0.885024	1.16E-71	-1.69E-46
MS.gene050612	MS.gene30542	0.908015	2.71E-81	-1.69E-46
MS.gene050662	MS.gene30542	0.811615	6.41E-51	-1.69E-46
MS.gene050675	MS.gene30542	0.889084	3.32E-73	-1.69E-46
MS.gene050699	MS.gene30542	0.886893	2.30E-72	-1.69E-46
MS.gene050747	MS.gene30542	0.835991	1.22E-56	-1.69E-46
MS.gene050772	MS.gene30542	0.860669	1.76E-63	-1.69E-46
MS.gene05080	MS.gene30542	0.834522	2.86E-56	-1.69E-46
MS.gene050868	MS.gene30542	0.88504	1.15E-71	-1.69E-46
MS.gene050871	MS.gene30542	0.886984	2.13E-72	-1.69E-46
MS.gene050876	MS.gene30542	0.807539	4.81E-50	-1.69E-46
MS.gene050883	MS.gene30542	-0.80778	4.28E-50	-1.69E-46
MS.gene050904	MS.gene30542	0.818638	1.77E-52	-1.69E-46
MS.gene050950	MS.gene30542	0.834377	3.10E-56	-1.69E-46
MS.gene050986	MS.gene30542	0.912664	1.50E-83	-1.69E-46
MS.gene05100	MS.gene30542	0.846062	2.75E-59	-1.69E-46
MS.gene05104	MS.gene30542	-0.819147	1.35E-52	-1.69E-46
MS.gene051127	MS.gene30542	-0.830588	2.70E-55	-1.69E-46
MS.gene051138	MS.gene30542	-0.809479	1.85E-50	-1.69E-46
MS.gene051167	MS.gene30542	-0.802691	4.98E-49	-1.69E-46
MS.gene051228	MS.gene30542	0.808106	3.64E-50	-1.69E-46
MS.gene051268	MS.gene30542	0.842275	2.86E-58	-1.69E-46
MS.gene051317	MS.gene30542	0.858942	5.82E-63	-1.69E-46
MS.gene05141	MS.gene30542	-0.806663	7.37E-50	-1.69E-46
MS.gene051509	MS.gene30542	0.82774	1.32E-54	-1.69E-46
MS.gene051551	MS.gene30542	0.889801	1.75E-73	-1.69E-46
MS.gene051651	MS.gene30542	-0.809074	2.26E-50	-1.69E-46
MS.gene051694	MS.gene30542	0.808308	3.30E-50	-1.69E-46
MS.gene051810	MS.gene30542	0.867724	1.11E-65	-1.69E-46
MS.gene051838	MS.gene30542	0.814131	1.80E-51	-1.69E-46
MS.gene051858	MS.gene30542	0.811093	8.32E-51	-1.69E-46
MS.gene051863	MS.gene30542	-0.803264	3.79E-49	-1.69E-46
MS.gene051949	MS.gene30542	0.815107	1.10E-51	-1.69E-46
MS.gene052164	MS.gene30542	0.879949	8.20E-70	-1.69E-46
MS.gene052271	MS.gene30542	0.809924	1.49E-50	-1.69E-46
MS.gene052379	MS.gene30542	0.815117	1.09E-51	-1.69E-46
MS.gene052482	MS.gene30542	0.853287	2.64E-61	-1.69E-46
MS.gene052486	MS.gene30542	0.854621	1.09E-61	-1.69E-46
MS.gene052503	MS.gene30542	0.870562	1.33E-66	-1.69E-46
MS.gene052504	MS.gene30542	0.803126	4.05E-49	-1.69E-46
MS.gene052506	MS.gene30542	0.820788	5.70E-53	-1.69E-46
MS.gene052615	MS.gene30542	0.847824	9.06E-60	-1.69E-46
MS.gene052822	MS.gene30542	0.835807	1.35E-56	-1.69E-46
MS.gene052823	MS.gene30542	0.878511	2.65E-69	-1.69E-46
MS.gene052825	MS.gene30542	0.867107	1.75E-65	-1.69E-46
MS.gene052872	MS.gene30542	0.818793	1.63E-52	-1.69E-46
MS.gene052916	MS.gene30542	0.802676	5.01E-49	-1.69E-46
MS.gene052966	MS.gene30542	0.835491	1.63E-56	-1.69E-46
MS.gene053018	MS.gene30542	0.801451	8.95E-49	-1.69E-46
MS.gene053117	MS.gene30542	0.80175	7.77E-49	-1.69E-46
MS.gene053203	MS.gene30542	0.874696	5.52E-68	-1.69E-46
MS.gene053207	MS.gene30542	0.824425	8.13E-54	-1.69E-46
MS.gene053213	MS.gene30542	0.880698	4.43E-70	-1.69E-46
MS.gene053286	MS.gene30542	0.886478	3.30E-72	-1.69E-46
MS.gene053420	MS.gene30542	-0.834179	3.48E-56	-1.69E-46
MS.gene053421	MS.gene30542	-0.813	3.19E-51	-1.69E-46
MS.gene053436	MS.gene30542	0.847773	9.35E-60	-1.69E-46
MS.gene053479	MS.gene30542	0.833803	4.32E-56	-1.69E-46
MS.gene053480	MS.gene30542	0.869047	4.15E-66	-1.69E-46
MS.gene053574	MS.gene30542	0.856825	2.48E-62	-1.69E-46
MS.gene053626	MS.gene30542	0.833369	5.55E-56	-1.69E-46
MS.gene053632	MS.gene30542	0.872705	2.59E-67	-1.69E-46
MS.gene053633	MS.gene30542	0.803954	2.72E-49	-1.69E-46
MS.gene053824	MS.gene30542	0.842497	2.49E-58	-1.69E-46
MS.gene053916	MS.gene30542	0.849708	2.72E-60	-1.69E-46
MS.gene054003	MS.gene30542	0.840577	8.00E-58	-1.69E-46
MS.gene054408	MS.gene30542	0.820361	7.15E-53	-1.69E-46
MS.gene054595	MS.gene30542	0.880411	5.61E-70	-1.69E-46
MS.gene054600	MS.gene30542	0.852084	5.82E-61	-1.69E-46
MS.gene054642	MS.gene30542	0.887503	1.35E-72	-1.69E-46
MS.gene054706	MS.gene30542	-0.831926	1.27E-55	-1.69E-46
MS.gene05470	MS.gene30542	0.8877	1.13E-72	-1.69E-46
MS.gene054927	MS.gene30542	0.803848	2.87E-49	-1.69E-46
MS.gene054954	MS.gene30542	0.918187	2.12E-86	-1.69E-46
MS.gene05505	MS.gene30542	0.824421	8.15E-54	-1.69E-46
MS.gene055154	MS.gene30542	-0.824643	7.22E-54	-1.69E-46
MS.gene055206	MS.gene30542	0.826859	2.15E-54	-1.69E-46
MS.gene055212	MS.gene30542	0.817993	2.47E-52	-1.69E-46
MS.gene055289	MS.gene30542	0.804674	1.93E-49	-1.69E-46
MS.gene055362	MS.gene30542	0.909473	5.48E-82	-1.69E-46
MS.gene055502	MS.gene30542	0.895261	1.13E-75	-1.69E-46
MS.gene055577	MS.gene30542	0.863699	2.07E-64	-1.69E-46
MS.gene055629	MS.gene30542	0.899652	1.60E-77	-1.69E-46
MS.gene055722	MS.gene30542	0.822253	2.62E-53	-1.69E-46
MS.gene055748	MS.gene30542	0.853029	3.13E-61	-1.69E-46
MS.gene055750	MS.gene30542	0.840764	7.14E-58	-1.69E-46
MS.gene055751	MS.gene30542	0.856189	3.81E-62	-1.69E-46
MS.gene055776	MS.gene30542	0.88051	5.17E-70	-1.69E-46
MS.gene05589	MS.gene30542	0.873248	1.70E-67	-1.69E-46
MS.gene05592	MS.gene30542	0.813645	2.30E-51	-1.69E-46
MS.gene055941	MS.gene30542	0.847888	8.70E-60	-1.69E-46
MS.gene056040	MS.gene30542	0.811484	6.84E-51	-1.69E-46
MS.gene056046	MS.gene30542	0.825838	3.77E-54	-1.69E-46
MS.gene056083	MS.gene30542	0.830628	2.64E-55	-1.69E-46
MS.gene056224	MS.gene30542	-0.802481	5.50E-49	-1.69E-46
MS.gene056374	MS.gene30542	0.862306	5.56E-64	-1.69E-46
MS.gene056387	MS.gene30542	0.856529	3.03E-62	-1.69E-46
MS.gene056446	MS.gene30542	0.834174	3.49E-56	-1.69E-46
MS.gene056487	MS.gene30542	0.840128	1.05E-57	-1.69E-46
MS.gene056504	MS.gene30542	0.913986	3.25E-84	-1.69E-46
MS.gene056510	MS.gene30542	0.892048	2.27E-74	-1.69E-46
MS.gene056657	MS.gene30542	0.907806	3.40E-81	-1.69E-46
MS.gene056692	MS.gene30542	0.844851	5.85E-59	-1.69E-46
MS.gene056878	MS.gene30542	0.814071	1.86E-51	-1.69E-46
MS.gene056898	MS.gene30542	0.839096	1.94E-57	-1.69E-46
MS.gene057014	MS.gene30542	-0.801757	7.75E-49	-1.69E-46
MS.gene057076	MS.gene30542	0.813012	3.18E-51	-1.69E-46
MS.gene057086	MS.gene30542	0.826798	2.23E-54	-1.69E-46
MS.gene057416	MS.gene30542	0.852689	3.91E-61	-1.69E-46
MS.gene05753	MS.gene30542	0.808941	2.42E-50	-1.69E-46
MS.gene057658	MS.gene30542	0.839427	1.59E-57	-1.69E-46
MS.gene057831	MS.gene30542	0.850941	1.23E-60	-1.69E-46
MS.gene057856	MS.gene30542	0.805213	1.49E-49	-1.69E-46
MS.gene057857	MS.gene30542	0.816829	4.52E-52	-1.69E-46
MS.gene05787	MS.gene30542	0.839209	1.82E-57	-1.69E-46
MS.gene057926	MS.gene30542	0.814351	1.61E-51	-1.69E-46
MS.gene058099	MS.gene30542	-0.803754	3.00E-49	-1.69E-46
MS.gene058130	MS.gene30542	0.809117	2.22E-50	-1.69E-46
MS.gene058157	MS.gene30542	0.843514	1.34E-58	-1.69E-46
MS.gene058171	MS.gene30542	-0.814944	1.19E-51	-1.69E-46
MS.gene058173	MS.gene30542	-0.803036	4.22E-49	-1.69E-46
MS.gene058175	MS.gene30542	-0.816885	4.39E-52	-1.69E-46
MS.gene058268	MS.gene30542	-0.801005	1.10E-48	-1.69E-46
MS.gene058269	MS.gene30542	-0.81127	7.62E-51	-1.69E-46
MS.gene058334	MS.gene30542	-0.821879	3.19E-53	-1.69E-46
MS.gene058338	MS.gene30542	0.815755	7.86E-52	-1.69E-46
MS.gene05837	MS.gene30542	0.835441	1.68E-56	-1.69E-46
MS.gene058419	MS.gene30542	0.901896	1.68E-78	-1.69E-46
MS.gene058544	MS.gene30542	0.881354	2.57E-70	-1.69E-46
MS.gene058719	MS.gene30542	0.81415	1.78E-51	-1.69E-46
MS.gene058723	MS.gene30542	0.834125	3.59E-56	-1.69E-46
MS.gene058773	MS.gene30542	0.869575	2.79E-66	-1.69E-46
MS.gene058838	MS.gene30542	0.839231	1.79E-57	-1.69E-46
MS.gene05883	MS.gene30542	0.806426	8.27E-50	-1.69E-46
MS.gene059022	MS.gene30542	0.910245	2.32E-82	-1.69E-46
MS.gene059023	MS.gene30542	0.85515	7.65E-62	-1.69E-46
MS.gene059029	MS.gene30542	0.803453	3.46E-49	-1.69E-46
MS.gene05913	MS.gene30542	0.852003	6.14E-61	-1.69E-46
MS.gene059223	MS.gene30542	-0.812712	3.69E-51	-1.69E-46
MS.gene059305	MS.gene30542	0.810176	1.31E-50	-1.69E-46
MS.gene05934	MS.gene30542	0.819092	1.39E-52	-1.69E-46
MS.gene059367	MS.gene30542	0.855896	4.64E-62	-1.69E-46
MS.gene059667	MS.gene30542	0.814624	1.40E-51	-1.69E-46
MS.gene059677	MS.gene30542	0.907578	4.36E-81	-1.69E-46
MS.gene059699	MS.gene30542	-0.823685	1.21E-53	-1.69E-46
MS.gene059701	MS.gene30542	-0.804297	2.31E-49	-1.69E-46
MS.gene059703	MS.gene30542	-0.836281	1.03E-56	-1.69E-46
MS.gene059718	MS.gene30542	0.885181	1.01E-71	-1.69E-46
MS.gene059756	MS.gene30542	0.848656	5.33E-60	-1.69E-46
MS.gene059801	MS.gene30542	0.804341	2.26E-49	-1.69E-46
MS.gene059865	MS.gene30542	0.905873	2.71E-80	-1.69E-46
MS.gene059902	MS.gene30542	0.809736	1.63E-50	-1.69E-46
MS.gene06000	MS.gene30542	0.858326	8.90E-63	-1.69E-46
MS.gene060341	MS.gene30542	0.824561	7.55E-54	-1.69E-46
MS.gene060482	MS.gene30542	0.839732	1.33E-57	-1.69E-46
MS.gene060595	MS.gene30542	-0.802876	4.56E-49	-1.69E-46
MS.gene060602	MS.gene30542	-0.803429	3.50E-49	-1.69E-46
MS.gene060703	MS.gene30542	0.856635	2.82E-62	-1.69E-46
MS.gene060728	MS.gene30542	0.830058	3.64E-55	-1.69E-46
MS.gene060762	MS.gene30542	0.88986	1.66E-73	-1.69E-46
MS.gene060778	MS.gene30542	0.883105	5.94E-71	-1.69E-46
MS.gene06077	MS.gene30542	0.89091	6.42E-74	-1.69E-46
MS.gene060835	MS.gene30542	0.813622	2.33E-51	-1.69E-46
MS.gene06092	MS.gene30542	0.829382	5.31E-55	-1.69E-46
MS.gene06097	MS.gene30542	0.814873	1.23E-51	-1.69E-46
MS.gene060982	MS.gene30542	0.87766	5.26E-69	-1.69E-46
MS.gene061134	MS.gene30542	0.846633	1.92E-59	-1.69E-46
MS.gene061150	MS.gene30542	0.851084	1.12E-60	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30542.t1	MTR_4g064963	93.333	165	8	1	1	162	1	165	9.19e-109	307
MS.gene30542.t1	MTR_4g065660	77.852	149	30	3	14	162	8	153	1.05e-67	202
MS.gene30542.t1	MTR_4g065123	72.662	139	36	2	12	150	1	137	1.51e-58	179
MS.gene30542.t1	MTR_7g066070	45.139	144	72	2	14	156	2	139	4.53e-38	127
MS.gene30542.t1	MTR_4g019870	47.899	119	58	1	40	154	13	131	4.26e-36	122
MS.gene30542.t1	MTR_0027s0220	51.240	121	53	4	21	138	11	128	1.46e-29	105
MS.gene30542.t1	MTR_1g015000	32.639	144	72	4	39	162	1	139	1.24e-16	72.4
MS.gene30542.t1	MTR_3g105330	29.078	141	73	3	39	156	1	137	2.92e-13	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30542.t1	AT2G26975	50.340	147	67	3	12	156	1	143	3.15e-42	138
MS.gene30542.t1	AT5G59030	50.806	124	57	2	43	162	45	168	1.88e-35	121
MS.gene30542.t1	AT3G46900	48.333	120	60	2	44	161	34	153	2.57e-34	118
MS.gene30542.t1	AT5G59040	43.651	126	71	0	34	159	24	149	1.42e-32	113
MS.gene30542.t1	AT2G37925	48.544	103	50	1	39	138	29	131	2.63e-29	105
MS.gene30542.t1	AT5G20650	34.400	125	57	1	39	138	1	125	9.05e-15	67.8

Find 42 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAGGGTTGGGTTAGCTTATT+TGG	0.161485	4.2:+40319813	MS.gene30542:CDS
ACCCATCATCATGGTGTTAT+TGG	0.242191	4.2:-40319569	None:intergenic
GTTGATGATGATGATGGTTT+TGG	0.274914	4.2:-40319547	None:intergenic
TCTTGTGTAGCAGTGCTATT+AGG	0.286924	4.2:+40319908	MS.gene30542:CDS
TGATGCACATGACATTCTTC+TGG	0.295699	4.2:+40319608	MS.gene30542:CDS
TAAGCCTTATCATCATGTTT+TGG	0.336110	4.2:-40319934	None:intergenic
CTTTAATTCTCTTCAACAAT+TGG	0.349322	4.2:+40319641	MS.gene30542:CDS
GCTGTGCTTGGTCATGCTCT+TGG	0.350764	4.2:+40319880	MS.gene30542:CDS
TTGGCTCTTATGTCTTTCAA+TGG	0.387989	4.2:+40319841	MS.gene30542:CDS
GTTAGCTTATTTGGTTATGT+TGG	0.388968	4.2:+40319822	MS.gene30542:CDS
GGCAGCAACATGGTTCGAAC+CGG	0.422987	4.2:-40319761	None:intergenic
GAAACCAAAACATGATGATA+AGG	0.457740	4.2:+40319930	MS.gene30542:CDS
GATGCACATGACATTCTTCT+GGG	0.470744	4.2:+40319609	MS.gene30542:CDS
AACCAATAACACCATGATGA+TGG	0.477184	4.2:+40319567	MS.gene30542:CDS
CTCTTCAACAATTGGCCTAA+CGG	0.488088	4.2:+40319649	MS.gene30542:CDS
ATGCACGGTGGCGGCATGGA+TGG	0.492115	4.2:+40319511	MS.gene30542:CDS
AATTGGCCTAACGGTGATAC+CGG	0.503435	4.2:+40319658	MS.gene30542:CDS
CAAAAGTGTCTGAAACAAAC+CGG	0.510839	4.2:-40319782	None:intergenic
GGTTCGAACCATGTTGCTGC+CGG	0.517508	4.2:+40319763	MS.gene30542:CDS
TTATTGGTTGATGATGATGA+TGG	0.527547	4.2:-40319553	None:intergenic
TGATACCGGTATGTATGTAT+TGG	0.532754	4.2:+40319672	MS.gene30542:CDS
ACCAATAACACCATGATGAT+GGG	0.534019	4.2:+40319568	MS.gene30542:CDS
GGTGGTAACATGCACGGTGG+CGG	0.534398	4.2:+40319502	MS.gene30542:CDS
GCTCTTATGTCTTTCAATGG+TGG	0.539076	4.2:+40319844	MS.gene30542:CDS
GATCTCACATCCATGGATCA+TGG	0.541471	4.2:+40319481	None:intergenic
TGAGAGTAATTCAATGAGTA+CGG	0.561035	4.2:-40319719	None:intergenic
GCAGCAACATGGTTCGAACC+GGG	0.567283	4.2:-40319760	None:intergenic
CATGTTACCACCATGATCCA+TGG	0.570126	4.2:-40319491	None:intergenic
CATCTTATGACCCATCATCA+TGG	0.576922	4.2:-40319578	None:intergenic
TTGAATTACTCTCACGTACA+CGG	0.584631	4.2:+40319728	MS.gene30542:CDS
CAACGCCAATACATACATAC+CGG	0.590800	4.2:-40319677	None:intergenic
TAACATGCACGGTGGCGGCA+TGG	0.592169	4.2:+40319507	MS.gene30542:CDS
CGTACACGGTTCATTAAACC+CGG	0.599386	4.2:+40319742	MS.gene30542:CDS
GAAACAAACCGGCAGCAACA+TGG	0.616307	4.2:-40319771	None:intergenic
ATGATGGGTCATAAGATGCA+CGG	0.627281	4.2:+40319583	MS.gene30542:CDS
GTACGGACATGACGAAAACA+AGG	0.653972	4.2:-40319702	None:intergenic
AACAATTAACAAGACAAGGG+AGG	0.655863	4.2:-40319964	None:intergenic
TACATACCGGTATCACCGTT+AGG	0.669036	4.2:-40319664	None:intergenic
ATGCACATGACATTCTTCTG+GGG	0.688076	4.2:+40319610	MS.gene30542:CDS
GATCATGGTGGTAACATGCA+CGG	0.714076	4.2:+40319496	MS.gene30542:CDS
CTCACATCCATGGATCATGG+TGG	0.733160	4.2:+40319484	None:intergenic
CATGGTGGTAACATGCACGG+TGG	0.787274	4.2:+40319499	MS.gene30542:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CTTTAATTCTCTTCAACAAT+TGG	+	chr4.2:40319641-40319660	MS.gene30542:CDS	25.0%
	GAAACCAAAACATGATGATA+AGG	+	chr4.2:40319930-40319949	MS.gene30542:CDS	30.0%
	TGAGAGTAATTCAATGAGTA+CGG	-	chr4.2:40319722-40319741	None:intergenic	30.0%
!	GTTAGCTTATTTGGTTATGT+TGG	+	chr4.2:40319822-40319841	MS.gene30542:CDS	30.0%
!!	TAAGCCTTATCATCATGTTT+TGG	-	chr4.2:40319937-40319956	None:intergenic	30.0%
!!	TTATTGGTTGATGATGATGA+TGG	-	chr4.2:40319556-40319575	None:intergenic	30.0%
	AACCAATAACACCATGATGA+TGG	+	chr4.2:40319567-40319586	MS.gene30542:CDS	35.0%
	ACCAATAACACCATGATGAT+GGG	+	chr4.2:40319568-40319587	MS.gene30542:CDS	35.0%
	TGATACCGGTATGTATGTAT+TGG	+	chr4.2:40319672-40319691	MS.gene30542:CDS	35.0%
	TTGAATTACTCTCACGTACA+CGG	+	chr4.2:40319728-40319747	MS.gene30542:CDS	35.0%
!	AGACACTTTTGCATGTTCTT+AGG	+	chr4.2:40319794-40319813	MS.gene30542:CDS	35.0%
!	GACACTTTTGCATGTTCTTA+GGG	+	chr4.2:40319795-40319814	MS.gene30542:CDS	35.0%
!	TTGGCTCTTATGTCTTTCAA+TGG	+	chr4.2:40319841-40319860	MS.gene30542:CDS	35.0%
!	TTTTGCATGTTCTTAGGGTT+GGG	+	chr4.2:40319800-40319819	MS.gene30542:CDS	35.0%
!!	CAAAAGTGTCTGAAACAAAC+CGG	-	chr4.2:40319785-40319804	None:intergenic	35.0%
!!	TTTCAATGGTGGTGTGTTTT+TGG	+	chr4.2:40319855-40319874	MS.gene30542:CDS	35.0%
!!!	GTTGATGATGATGATGGTTT+TGG	-	chr4.2:40319550-40319569	None:intergenic	35.0%
	ATGCACATGACATTCTTCTG+GGG	+	chr4.2:40319610-40319629	MS.gene30542:CDS	40.0%
	CAACGCCAATACATACATAC+CGG	-	chr4.2:40319680-40319699	None:intergenic	40.0%
	CATCTTATGACCCATCATCA+TGG	-	chr4.2:40319581-40319600	None:intergenic	40.0%
	GATGCACATGACATTCTTCT+GGG	+	chr4.2:40319609-40319628	MS.gene30542:CDS	40.0%
	GCTCTTATGTCTTTCAATGG+TGG	+	chr4.2:40319844-40319863	MS.gene30542:CDS	40.0%
	TGATGCACATGACATTCTTC+TGG	+	chr4.2:40319608-40319627	MS.gene30542:CDS	40.0%
!	CTCTTCAACAATTGGCCTAA+CGG	+	chr4.2:40319649-40319668	MS.gene30542:CDS	40.0%
!	CTTTTGCATGTTCTTAGGGT+TGG	+	chr4.2:40319799-40319818	MS.gene30542:CDS	40.0%
!	TCTTGTGTAGCAGTGCTATT+AGG	+	chr4.2:40319908-40319927	MS.gene30542:CDS	40.0%
!!	ACCCATCATCATGGTGTTAT+TGG	-	chr4.2:40319572-40319591	None:intergenic	40.0%
!!	ATGATGGGTCATAAGATGCA+CGG	+	chr4.2:40319583-40319602	MS.gene30542:CDS	40.0%
!!	TAGGGTTGGGTTAGCTTATT+TGG	+	chr4.2:40319813-40319832	MS.gene30542:CDS	40.0%
!!!	GATGATGATGATGGTTTTGG+TGG	-	chr4.2:40319547-40319566	None:intergenic	40.0%
	CGTACACGGTTCATTAAACC+CGG	+	chr4.2:40319742-40319761	MS.gene30542:CDS	45.0%
	GATCATGGTGGTAACATGCA+CGG	+	chr4.2:40319496-40319515	MS.gene30542:CDS	45.0%
	GTACGGACATGACGAAAACA+AGG	-	chr4.2:40319705-40319724	None:intergenic	45.0%
	TACATACCGGTATCACCGTT+AGG	-	chr4.2:40319667-40319686	None:intergenic	45.0%
!	AATTGGCCTAACGGTGATAC+CGG	+	chr4.2:40319658-40319677	MS.gene30542:CDS	45.0%
!!!	GTGTTTTTGGTTGCTGTGCT+TGG	+	chr4.2:40319868-40319887	MS.gene30542:CDS	45.0%
	GAAACAAACCGGCAGCAACA+TGG	-	chr4.2:40319774-40319793	None:intergenic	50.0%
	CATGGTGGTAACATGCACGG+TGG	+	chr4.2:40319499-40319518	MS.gene30542:CDS	55.0%
	GCAGCAACATGGTTCGAACC+GGG	-	chr4.2:40319763-40319782	None:intergenic	55.0%
	GCTGTGCTTGGTCATGCTCT+TGG	+	chr4.2:40319880-40319899	MS.gene30542:CDS	55.0%
	GGCAGCAACATGGTTCGAAC+CGG	-	chr4.2:40319764-40319783	None:intergenic	55.0%
	GGTTCGAACCATGTTGCTGC+CGG	+	chr4.2:40319763-40319782	MS.gene30542:CDS	55.0%
	GGTGGTAACATGCACGGTGG+CGG	+	chr4.2:40319502-40319521	MS.gene30542:CDS	60.0%
!	TAACATGCACGGTGGCGGCA+TGG	+	chr4.2:40319507-40319526	MS.gene30542:CDS	60.0%
!	ATGCACGGTGGCGGCATGGA+TGG	+	chr4.2:40319511-40319530	MS.gene30542:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	40319493	40319981	40319493	ID=MS.gene30542
chr4.2	mRNA	40319493	40319981	40319493	ID=MS.gene30542.t1;Parent=MS.gene30542
chr4.2	exon	40319493	40319981	40319493	ID=MS.gene30542.t1.exon1;Parent=MS.gene30542.t1
chr4.2	CDS	40319493	40319981	40319493	ID=cds.MS.gene30542.t1;Parent=MS.gene30542.t1

Gene Sequence

>MS.gene30542

ATGGATCATGGTGGTAACATGCACGGTGGCGGCATGGATGGAATGAACATGCCACCAAAACCATCATCATCATCAACCAATAACACCATGATGATGGGTCATAAGATGCACGGCATGATGCACATGACATTCTTCTGGGGCAAAGATGCTTTAATTCTCTTCAACAATTGGCCTAACGGTGATACCGGTATGTATGTATTGGCGTTGATCCTTGTTTTCGTCATGTCCGTACTCATTGAATTACTCTCACGTACACGGTTCATTAAACCCGGTTCGAACCATGTTGCTGCCGGTTTGTTTCAGACACTTTTGCATGTTCTTAGGGTTGGGTTAGCTTATTTGGTTATGTTGGCTCTTATGTCTTTCAATGGTGGTGTGTTTTTGGTTGCTGTGCTTGGTCATGCTCTTGGATTCTTCTTGTGTAGCAGTGCTATTAGGAAACCAAAACATGATGATAAGGCTTATGATCTTCCTCCCTTGTCTTGTTAA

Protein sequence

>MS.gene30542.t1

MDHGGNMHGGGMDGMNMPPKPSSSSTNNTMMMGHKMHGMMHMTFFWGKDALILFNNWPNGDTGMYVLALILVFVMSVLIELLSRTRFIKPGSNHVAAGLFQTLLHVLRVGLAYLVMLALMSFNGGVFLVAVLGHALGFFLCSSAIRKPKHDDKAYDLPPLSC