Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33754.t1 | XP_013457084.1 | 96.9 | 193 | 6 | 0 | 1 | 193 | 1 | 193 | 3.00E-42 | 181.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33754.t1 | I3STZ6 | 96.9 | 193 | 6 | 0 | 1 | 193 | 1 | 193 | 2.2e-42 | 181.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene33754 | 0.806914 | 6.53E-50 | -1.69E-46 |
| MS.gene049171 | MS.gene33754 | 0.8951 | 1.32E-75 | -1.69E-46 |
| MS.gene049173 | MS.gene33754 | 0.904551 | 1.09E-79 | -1.69E-46 |
| MS.gene049217 | MS.gene33754 | 0.8738 | 1.11E-67 | -1.69E-46 |
| MS.gene049222 | MS.gene33754 | 0.880592 | 4.83E-70 | -1.69E-46 |
| MS.gene049232 | MS.gene33754 | 0.802037 | 6.78E-49 | -1.69E-46 |
| MS.gene049285 | MS.gene33754 | 0.908267 | 2.06E-81 | -1.69E-46 |
| MS.gene049320 | MS.gene33754 | 0.888633 | 4.96E-73 | -1.69E-46 |
| MS.gene04939 | MS.gene33754 | 0.835778 | 1.38E-56 | -1.69E-46 |
| MS.gene049427 | MS.gene33754 | 0.834997 | 2.17E-56 | -1.69E-46 |
| MS.gene049609 | MS.gene33754 | 0.837575 | 4.79E-57 | -1.69E-46 |
| MS.gene049614 | MS.gene33754 | 0.819601 | 1.07E-52 | -1.69E-46 |
| MS.gene049627 | MS.gene33754 | 0.813401 | 2.61E-51 | -1.69E-46 |
| MS.gene049633 | MS.gene33754 | 0.850621 | 1.51E-60 | -1.69E-46 |
| MS.gene049805 | MS.gene33754 | 0.863276 | 2.79E-64 | -1.69E-46 |
| MS.gene05010 | MS.gene33754 | 0.822192 | 2.70E-53 | -1.69E-46 |
| MS.gene050163 | MS.gene33754 | 0.916877 | 1.05E-85 | -1.69E-46 |
| MS.gene050165 | MS.gene33754 | 0.90406 | 1.82E-79 | -1.69E-46 |
| MS.gene050323 | MS.gene33754 | 0.816993 | 4.15E-52 | -1.69E-46 |
| MS.gene050333 | MS.gene33754 | 0.869513 | 2.93E-66 | -1.69E-46 |
| MS.gene050353 | MS.gene33754 | -0.813181 | 2.91E-51 | -1.69E-46 |
| MS.gene050432 | MS.gene33754 | 0.815121 | 1.09E-51 | -1.69E-46 |
| MS.gene050444 | MS.gene33754 | 0.867562 | 1.25E-65 | -1.69E-46 |
| MS.gene050504 | MS.gene33754 | 0.891009 | 5.87E-74 | -1.69E-46 |
| MS.gene050518 | MS.gene33754 | 0.873277 | 1.67E-67 | -1.69E-46 |
| MS.gene050538 | MS.gene33754 | 0.814454 | 1.53E-51 | -1.69E-46 |
| MS.gene050577 | MS.gene33754 | 0.841094 | 5.85E-58 | -1.69E-46 |
| MS.gene050612 | MS.gene33754 | 0.888328 | 6.51E-73 | -1.69E-46 |
| MS.gene050675 | MS.gene33754 | 0.845637 | 3.59E-59 | -1.69E-46 |
| MS.gene050699 | MS.gene33754 | 0.86452 | 1.15E-64 | -1.69E-46 |
| MS.gene050747 | MS.gene33754 | 0.839517 | 1.51E-57 | -1.69E-46 |
| MS.gene050767 | MS.gene33754 | 0.830762 | 2.45E-55 | -1.69E-46 |
| MS.gene050770 | MS.gene33754 | 0.833216 | 6.06E-56 | -1.69E-46 |
| MS.gene050772 | MS.gene33754 | 0.86129 | 1.14E-63 | -1.69E-46 |
| MS.gene05080 | MS.gene33754 | 0.827874 | 1.23E-54 | -1.69E-46 |
| MS.gene05081 | MS.gene33754 | 0.802247 | 6.14E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene33754 | 0.816894 | 4.37E-52 | -1.69E-46 |
| MS.gene050871 | MS.gene33754 | 0.815536 | 8.79E-52 | -1.69E-46 |
| MS.gene050876 | MS.gene33754 | 0.815286 | 1.00E-51 | -1.69E-46 |
| MS.gene050950 | MS.gene33754 | 0.837748 | 4.33E-57 | -1.69E-46 |
| MS.gene050986 | MS.gene33754 | 0.871721 | 5.51E-67 | -1.69E-46 |
| MS.gene05100 | MS.gene33754 | 0.885168 | 1.03E-71 | -1.69E-46 |
| MS.gene051228 | MS.gene33754 | 0.893751 | 4.70E-75 | -1.69E-46 |
| MS.gene051317 | MS.gene33754 | 0.837783 | 4.24E-57 | -1.69E-46 |
| MS.gene051551 | MS.gene33754 | 0.853165 | 2.86E-61 | -1.69E-46 |
| MS.gene051617 | MS.gene33754 | 0.834999 | 2.17E-56 | -1.69E-46 |
| MS.gene051666 | MS.gene33754 | 0.855525 | 5.95E-62 | -1.69E-46 |
| MS.gene051692 | MS.gene33754 | 0.847351 | 1.22E-59 | -1.69E-46 |
| MS.gene051694 | MS.gene33754 | 0.811212 | 7.84E-51 | -1.69E-46 |
| MS.gene051810 | MS.gene33754 | 0.912007 | 3.19E-83 | -1.69E-46 |
| MS.gene051838 | MS.gene33754 | 0.806789 | 6.93E-50 | -1.69E-46 |
| MS.gene051858 | MS.gene33754 | 0.833067 | 6.60E-56 | -1.69E-46 |
| MS.gene051949 | MS.gene33754 | 0.861647 | 8.85E-64 | -1.69E-46 |
| MS.gene052164 | MS.gene33754 | 0.848704 | 5.17E-60 | -1.69E-46 |
| MS.gene052271 | MS.gene33754 | 0.888928 | 3.82E-73 | -1.69E-46 |
| MS.gene052290 | MS.gene33754 | 0.853505 | 2.29E-61 | -1.69E-46 |
| MS.gene052379 | MS.gene33754 | 0.887951 | 9.08E-73 | -1.69E-46 |
| MS.gene052481 | MS.gene33754 | 0.804473 | 2.12E-49 | -1.69E-46 |
| MS.gene052484 | MS.gene33754 | 0.847506 | 1.11E-59 | -1.69E-46 |
| MS.gene052486 | MS.gene33754 | 0.804148 | 2.48E-49 | -1.69E-46 |
| MS.gene052503 | MS.gene33754 | 0.820429 | 6.89E-53 | -1.69E-46 |
| MS.gene052615 | MS.gene33754 | 0.854165 | 1.48E-61 | -1.69E-46 |
| MS.gene052823 | MS.gene33754 | 0.809398 | 1.93E-50 | -1.69E-46 |
| MS.gene052825 | MS.gene33754 | 0.810928 | 9.03E-51 | -1.69E-46 |
| MS.gene052870 | MS.gene33754 | 0.846887 | 1.64E-59 | -1.69E-46 |
| MS.gene052872 | MS.gene33754 | 0.850948 | 1.22E-60 | -1.69E-46 |
| MS.gene052916 | MS.gene33754 | 0.804016 | 2.64E-49 | -1.69E-46 |
| MS.gene052966 | MS.gene33754 | 0.844432 | 7.59E-59 | -1.69E-46 |
| MS.gene053009 | MS.gene33754 | 0.842892 | 1.96E-58 | -1.69E-46 |
| MS.gene053103 | MS.gene33754 | 0.845666 | 3.52E-59 | -1.69E-46 |
| MS.gene053104 | MS.gene33754 | 0.861865 | 7.60E-64 | -1.69E-46 |
| MS.gene053203 | MS.gene33754 | 0.827157 | 1.83E-54 | -1.69E-46 |
| MS.gene053207 | MS.gene33754 | 0.877782 | 4.77E-69 | -1.69E-46 |
| MS.gene053213 | MS.gene33754 | 0.873227 | 1.73E-67 | -1.69E-46 |
| MS.gene053284 | MS.gene33754 | 0.824346 | 8.48E-54 | -1.69E-46 |
| MS.gene053285 | MS.gene33754 | 0.826666 | 2.39E-54 | -1.69E-46 |
| MS.gene053286 | MS.gene33754 | 0.874651 | 5.72E-68 | -1.69E-46 |
| MS.gene053322 | MS.gene33754 | 0.874305 | 7.49E-68 | -1.69E-46 |
| MS.gene053480 | MS.gene33754 | 0.80598 | 1.03E-49 | -1.69E-46 |
| MS.gene053574 | MS.gene33754 | 0.824472 | 7.92E-54 | -1.69E-46 |
| MS.gene053632 | MS.gene33754 | 0.90113 | 3.66E-78 | -1.69E-46 |
| MS.gene053824 | MS.gene33754 | 0.828396 | 9.20E-55 | -1.69E-46 |
| MS.gene053916 | MS.gene33754 | 0.830248 | 3.27E-55 | -1.69E-46 |
| MS.gene054376 | MS.gene33754 | 0.804918 | 1.72E-49 | -1.69E-46 |
| MS.gene054408 | MS.gene33754 | 0.833774 | 4.40E-56 | -1.69E-46 |
| MS.gene054595 | MS.gene33754 | 0.840269 | 9.63E-58 | -1.69E-46 |
| MS.gene054600 | MS.gene33754 | 0.907883 | 3.13E-81 | -1.69E-46 |
| MS.gene054642 | MS.gene33754 | 0.906017 | 2.32E-80 | -1.69E-46 |
| MS.gene05470 | MS.gene33754 | 0.834473 | 2.94E-56 | -1.69E-46 |
| MS.gene054954 | MS.gene33754 | 0.87805 | 3.84E-69 | -1.69E-46 |
| MS.gene05505 | MS.gene33754 | 0.807168 | 5.77E-50 | -1.69E-46 |
| MS.gene055206 | MS.gene33754 | 0.860509 | 1.97E-63 | -1.69E-46 |
| MS.gene055212 | MS.gene33754 | 0.85698 | 2.23E-62 | -1.69E-46 |
| MS.gene055254 | MS.gene33754 | 0.82074 | 5.85E-53 | -1.69E-46 |
| MS.gene055289 | MS.gene33754 | 0.802825 | 4.67E-49 | -1.69E-46 |
| MS.gene055362 | MS.gene33754 | 0.890148 | 1.28E-73 | -1.69E-46 |
| MS.gene055397 | MS.gene33754 | 0.85764 | 1.42E-62 | -1.69E-46 |
| MS.gene055412 | MS.gene33754 | 0.83206 | 1.17E-55 | -1.69E-46 |
| MS.gene055499 | MS.gene33754 | 0.866626 | 2.48E-65 | -1.69E-46 |
| MS.gene055502 | MS.gene33754 | 0.891644 | 3.29E-74 | -1.69E-46 |
| MS.gene055577 | MS.gene33754 | 0.861454 | 1.01E-63 | -1.69E-46 |
| MS.gene055636 | MS.gene33754 | 0.844997 | 5.34E-59 | -1.69E-46 |
| MS.gene055638 | MS.gene33754 | 0.839757 | 1.31E-57 | -1.69E-46 |
| MS.gene055647 | MS.gene33754 | 0.858257 | 9.33E-63 | -1.69E-46 |
| MS.gene055722 | MS.gene33754 | 0.867618 | 1.20E-65 | -1.69E-46 |
| MS.gene055748 | MS.gene33754 | 0.849518 | 3.07E-60 | -1.69E-46 |
| MS.gene055750 | MS.gene33754 | 0.813597 | 2.36E-51 | -1.69E-46 |
| MS.gene055751 | MS.gene33754 | 0.84346 | 1.38E-58 | -1.69E-46 |
| MS.gene055776 | MS.gene33754 | 0.821457 | 4.00E-53 | -1.69E-46 |
| MS.gene05589 | MS.gene33754 | 0.821977 | 3.03E-53 | -1.69E-46 |
| MS.gene05592 | MS.gene33754 | 0.840296 | 9.47E-58 | -1.69E-46 |
| MS.gene055941 | MS.gene33754 | 0.867844 | 1.01E-65 | -1.69E-46 |
| MS.gene055956 | MS.gene33754 | -0.82536 | 4.89E-54 | -1.69E-46 |
| MS.gene056083 | MS.gene33754 | 0.841529 | 4.50E-58 | -1.69E-46 |
| MS.gene056374 | MS.gene33754 | 0.898276 | 6.22E-77 | -1.69E-46 |
| MS.gene056449 | MS.gene33754 | 0.826912 | 2.09E-54 | -1.69E-46 |
| MS.gene056504 | MS.gene33754 | 0.843718 | 1.18E-58 | -1.69E-46 |
| MS.gene056510 | MS.gene33754 | 0.882461 | 1.02E-70 | -1.69E-46 |
| MS.gene056549 | MS.gene33754 | 0.820331 | 7.26E-53 | -1.69E-46 |
| MS.gene056609 | MS.gene33754 | 0.809686 | 1.67E-50 | -1.69E-46 |
| MS.gene056657 | MS.gene33754 | 0.892974 | 9.67E-75 | -1.69E-46 |
| MS.gene056692 | MS.gene33754 | 0.820418 | 6.93E-53 | -1.69E-46 |
| MS.gene056897 | MS.gene33754 | 0.874734 | 5.36E-68 | -1.69E-46 |
| MS.gene056898 | MS.gene33754 | 0.822889 | 1.86E-53 | -1.69E-46 |
| MS.gene057011 | MS.gene33754 | 0.814855 | 1.25E-51 | -1.69E-46 |
| MS.gene057076 | MS.gene33754 | 0.811318 | 7.43E-51 | -1.69E-46 |
| MS.gene057129 | MS.gene33754 | 0.890989 | 5.98E-74 | -1.69E-46 |
| MS.gene057246 | MS.gene33754 | 0.83087 | 2.30E-55 | -1.69E-46 |
| MS.gene057295 | MS.gene33754 | 0.822492 | 2.30E-53 | -1.69E-46 |
| MS.gene057331 | MS.gene33754 | 0.835084 | 2.06E-56 | -1.69E-46 |
| MS.gene057416 | MS.gene33754 | 0.87047 | 1.42E-66 | -1.69E-46 |
| MS.gene057658 | MS.gene33754 | 0.833339 | 5.65E-56 | -1.69E-46 |
| MS.gene057805 | MS.gene33754 | 0.804806 | 1.81E-49 | -1.69E-46 |
| MS.gene057831 | MS.gene33754 | 0.844366 | 7.90E-59 | -1.69E-46 |
| MS.gene057856 | MS.gene33754 | 0.801187 | 1.01E-48 | -1.69E-46 |
| MS.gene057857 | MS.gene33754 | 0.801373 | 9.28E-49 | -1.69E-46 |
| MS.gene057926 | MS.gene33754 | 0.828706 | 7.74E-55 | -1.69E-46 |
| MS.gene058006 | MS.gene33754 | 0.802503 | 5.44E-49 | -1.69E-46 |
| MS.gene058019 | MS.gene33754 | 0.826845 | 2.17E-54 | -1.69E-46 |
| MS.gene058323 | MS.gene33754 | 0.804026 | 2.63E-49 | -1.69E-46 |
| MS.gene058338 | MS.gene33754 | 0.810603 | 1.06E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene33754 | 0.897886 | 9.09E-77 | -1.69E-46 |
| MS.gene058544 | MS.gene33754 | 0.869442 | 3.09E-66 | -1.69E-46 |
| MS.gene058723 | MS.gene33754 | 0.858426 | 8.31E-63 | -1.69E-46 |
| MS.gene058773 | MS.gene33754 | 0.890955 | 6.17E-74 | -1.69E-46 |
| MS.gene058838 | MS.gene33754 | 0.851905 | 6.54E-61 | -1.69E-46 |
| MS.gene059022 | MS.gene33754 | 0.896425 | 3.74E-76 | -1.69E-46 |
| MS.gene059023 | MS.gene33754 | 0.857076 | 2.09E-62 | -1.69E-46 |
| MS.gene059029 | MS.gene33754 | 0.859929 | 2.94E-63 | -1.69E-46 |
| MS.gene05912 | MS.gene33754 | 0.809443 | 1.89E-50 | -1.69E-46 |
| MS.gene05913 | MS.gene33754 | 0.839016 | 2.04E-57 | -1.69E-46 |
| MS.gene05914 | MS.gene33754 | 0.813336 | 2.70E-51 | -1.69E-46 |
| MS.gene05934 | MS.gene33754 | 0.827443 | 1.56E-54 | -1.69E-46 |
| MS.gene059455 | MS.gene33754 | 0.803386 | 3.57E-49 | -1.69E-46 |
| MS.gene059536 | MS.gene33754 | 0.83811 | 3.49E-57 | -1.69E-46 |
| MS.gene059677 | MS.gene33754 | 0.903242 | 4.25E-79 | -1.69E-46 |
| MS.gene059718 | MS.gene33754 | 0.883163 | 5.66E-71 | -1.69E-46 |
| MS.gene059756 | MS.gene33754 | 0.830572 | 2.72E-55 | -1.69E-46 |
| MS.gene059801 | MS.gene33754 | 0.814182 | 1.75E-51 | -1.69E-46 |
| MS.gene059858 | MS.gene33754 | 0.837083 | 6.41E-57 | -1.69E-46 |
| MS.gene059865 | MS.gene33754 | 0.852051 | 5.95E-61 | -1.69E-46 |
| MS.gene059902 | MS.gene33754 | 0.824497 | 7.82E-54 | -1.69E-46 |
| MS.gene059960 | MS.gene33754 | 0.843965 | 1.01E-58 | -1.69E-46 |
| MS.gene060341 | MS.gene33754 | 0.865449 | 5.86E-65 | -1.69E-46 |
| MS.gene060356 | MS.gene33754 | 0.81119 | 7.93E-51 | -1.69E-46 |
| MS.gene060482 | MS.gene33754 | 0.806168 | 9.38E-50 | -1.69E-46 |
| MS.gene060703 | MS.gene33754 | 0.866676 | 2.39E-65 | -1.69E-46 |
| MS.gene060727 | MS.gene33754 | 0.852226 | 5.30E-61 | -1.69E-46 |
| MS.gene060728 | MS.gene33754 | 0.8506 | 1.53E-60 | -1.69E-46 |
| MS.gene060762 | MS.gene33754 | 0.87895 | 1.85E-69 | -1.69E-46 |
| MS.gene06077 | MS.gene33754 | 0.843106 | 1.72E-58 | -1.69E-46 |
| MS.gene060804 | MS.gene33754 | 0.832575 | 8.74E-56 | -1.69E-46 |
| MS.gene060818 | MS.gene33754 | 0.819562 | 1.09E-52 | -1.69E-46 |
| MS.gene06092 | MS.gene33754 | 0.853518 | 2.27E-61 | -1.69E-46 |
| MS.gene060982 | MS.gene33754 | 0.831766 | 1.39E-55 | -1.69E-46 |
| MS.gene061041 | MS.gene33754 | 0.816742 | 4.73E-52 | -1.69E-46 |
| MS.gene061134 | MS.gene33754 | 0.80218 | 6.34E-49 | -1.69E-46 |
| MS.gene061150 | MS.gene33754 | 0.89652 | 3.41E-76 | -1.69E-46 |
| MS.gene061200 | MS.gene33754 | 0.867663 | 1.16E-65 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33754.t1 | MTR_4g089080 | 97.409 | 193 | 5 | 0 | 1 | 193 | 1 | 193 | 2.42e-133 | 371 |
| MS.gene33754.t1 | MTR_3g026020 | 39.429 | 175 | 93 | 4 | 25 | 191 | 18 | 187 | 1.80e-20 | 84.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33754.t1 | AT1G74730 | 65.625 | 192 | 64 | 2 | 3 | 193 | 8 | 198 | 4.61e-67 | 204 |
| MS.gene33754.t1 | AT5G08050 | 40.000 | 125 | 67 | 2 | 72 | 193 | 39 | 158 | 6.02e-15 | 69.3 |
Find 49 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGGCTGTTGGGTTGTTTGC+TGG | 0.240768 | 4.4:-23527776 | MS.gene33754:CDS |
| TGTTGTTCTCGGCGGGTTGC+AGG | 0.251096 | 4.4:-23527750 | MS.gene33754:CDS |
| CTCTTGGTGTTGGGGCTGTT+GGG | 0.261115 | 4.4:-23527787 | MS.gene33754:CDS |
| GCTCTTGGTGTTGGGGCTGT+TGG | 0.285475 | 4.4:-23527788 | MS.gene33754:CDS |
| TACTACTCTTGTTGTAGTTC+AGG | 0.286824 | 4.4:-23527825 | MS.gene33754:CDS |
| TCGCTGCTGCTCTTGGTGTT+GGG | 0.299413 | 4.4:-23527796 | MS.gene33754:CDS |
| TCTGGCGCTTCCGACGCTTT+TGG | 0.312429 | 4.4:-23527885 | MS.gene33754:CDS |
| AAAGACGGTGAAAGGTTTAT+TGG | 0.327341 | 4.4:+23528287 | None:intergenic |
| GTCGCTGCTGCTCTTGGTGT+TGG | 0.327834 | 4.4:-23527797 | MS.gene33754:CDS |
| TCCTTCCGTCTTGGCTTCTC+TGG | 0.328777 | 4.4:-23527903 | MS.gene33754:CDS |
| TGAACAAGTACAACGATAAC+TGG | 0.365581 | 4.4:-23528159 | MS.gene33754:CDS |
| TTTGCTGGATCTGTTGTTCT+CGG | 0.382286 | 4.4:-23527761 | MS.gene33754:CDS |
| TTATCCAAAGCTGAAGAATT+CGG | 0.394470 | 4.4:-23528019 | MS.gene33754:CDS |
| GACGCCGAATTCTTCAGCTT+TGG | 0.394886 | 4.4:+23528015 | None:intergenic |
| CGCAGTCTCTCCTTCCGTCT+TGG | 0.401430 | 4.4:-23527912 | MS.gene33754:CDS |
| GCTGTTGTCGCTGCTGCTCT+TGG | 0.417121 | 4.4:-23527803 | MS.gene33754:CDS |
| CTGGATCTGTTGTTCTCGGC+GGG | 0.425288 | 4.4:-23527757 | MS.gene33754:CDS |
| CGCTGCTGCTCTTGGTGTTG+GGG | 0.467142 | 4.4:-23527795 | MS.gene33754:CDS |
| AAAGGTTTATTGGAGGAAGT+AGG | 0.473704 | 4.4:+23528297 | None:intergenic |
| AAGGTTTATTGGAGGAAGTA+GGG | 0.490170 | 4.4:+23528298 | None:intergenic |
| TGGAGAGCTGAGGTTGGAGC+AGG | 0.490180 | 4.4:+23528372 | None:intergenic |
| AACTGGAAGAAACAATGGTT+CGG | 0.517807 | 4.4:-23528142 | MS.gene33754:CDS |
| ACCTTTCACCGTCTTTGCCA+TGG | 0.518610 | 4.4:-23528280 | MS.gene33754:CDS |
| AAGAAACAATGGTTCGGAGC+AGG | 0.525520 | 4.4:-23528136 | MS.gene33754:CDS |
| GCCAGAGAAGCCAAGACGGA+AGG | 0.526449 | 4.4:+23527902 | None:intergenic |
| AGCGATAGTGACGGAGGGTG+CGG | 0.535126 | 4.4:+23528325 | None:intergenic |
| ACAAGAGTAGTAGAATCATC+AGG | 0.544351 | 4.4:+23527836 | None:intergenic |
| TTTGGGAGCCATGGCAAAGA+CGG | 0.549608 | 4.4:+23528272 | None:intergenic |
| GACGGTGAAAGGTTTATTGG+AGG | 0.550094 | 4.4:+23528290 | None:intergenic |
| GCCATGGCAAAGACGGTGAA+AGG | 0.551635 | 4.4:+23528279 | None:intergenic |
| AGGAAGTGAAGAAGTAGAAG+TGG | 0.552394 | 4.4:-23528101 | MS.gene33754:CDS |
| CTCTCTTCAATCGAGAAACT+AGG | 0.554038 | 4.4:-23527989 | MS.gene33754:CDS |
| AAGCGCCAGAGAAGCCAAGA+CGG | 0.557107 | 4.4:+23527898 | None:intergenic |
| TGTTCTCGGCGGGTTGCAGG+AGG | 0.559794 | 4.4:-23527747 | MS.gene33754:CDS |
| GGAGGGTGCGGTGGTTAGTG+TGG | 0.562291 | 4.4:+23528337 | None:intergenic |
| TTTGGCTGCTGTTGCGACGG+TGG | 0.563007 | 4.4:-23527867 | MS.gene33754:CDS |
| TAGTGTGGATGTGTTGAAGA+TGG | 0.568146 | 4.4:+23528352 | None:intergenic |
| TGAAGATGGAGAGCTGAGGT+TGG | 0.585555 | 4.4:+23528366 | None:intergenic |
| GGTTGGAGCAGGAACAGCCA+TGG | 0.616976 | 4.4:+23528383 | None:intergenic |
| GCTGGATCTGTTGTTCTCGG+CGG | 0.618325 | 4.4:-23527758 | MS.gene33754:CDS |
| ACGATAACTGGAAGAAACAA+TGG | 0.622411 | 4.4:-23528147 | MS.gene33754:CDS |
| CAAGACGGAAGGAGAGACTG+CGG | 0.624177 | 4.4:+23527913 | None:intergenic |
| GATAGTGACGGAGGGTGCGG+TGG | 0.630396 | 4.4:+23528328 | None:intergenic |
| TAGGGAATGAGCGATAGTGA+CGG | 0.635156 | 4.4:+23528316 | None:intergenic |
| GCAACAGCAGCCAAAAGCGT+CGG | 0.640980 | 4.4:+23527875 | None:intergenic |
| GGAATGAGCGATAGTGACGG+AGG | 0.658654 | 4.4:+23528319 | None:intergenic |
| GAATGAGCGATAGTGACGGA+GGG | 0.663461 | 4.4:+23528320 | None:intergenic |
| GGAGCAGGAATATTCTACGA+AGG | 0.698664 | 4.4:-23528121 | MS.gene33754:CDS |
| GTGTTGAAGATGGAGAGCTG+AGG | 0.722843 | 4.4:+23528362 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTATTTGAATTTATGTTAA+TGG | - | chr4.4:23527928-23527947 | MS.gene33754:CDS | 10.0% |
| !!! | TGAATTTATGTTAATGGATA+TGG | - | chr4.4:23527934-23527953 | MS.gene33754:CDS | 20.0% |
| ! | AAATAATGACGAAAATGATG+AGG | + | chr4.4:23527896-23527915 | None:intergenic | 25.0% |
| !! | TTTATGTTAATGGATATGGA+AGG | - | chr4.4:23527938-23527957 | MS.gene33754:CDS | 25.0% |
| TTATCCAAAGCTGAAGAATT+CGG | - | chr4.4:23528101-23528120 | MS.gene33754:CDS | 30.0% | |
| ! | TGTGTATGTACCTTCTTTTT+GGG | + | chr4.4:23527868-23527887 | None:intergenic | 30.0% |
| ! | TTGTGTATGTACCTTCTTTT+TGG | + | chr4.4:23527869-23527888 | None:intergenic | 30.0% |
| !!! | GAAACTAGGACTTTTTTCTA+AGG | - | chr4.4:23528145-23528164 | MS.gene33754:CDS | 30.0% |
| AAAGACGGTGAAAGGTTTAT+TGG | + | chr4.4:23527836-23527855 | None:intergenic | 35.0% | |
| AAAGGTTTATTGGAGGAAGT+AGG | + | chr4.4:23527826-23527845 | None:intergenic | 35.0% | |
| AACTGGAAGAAACAATGGTT+CGG | - | chr4.4:23527978-23527997 | MS.gene33754:CDS | 35.0% | |
| AAGGTTTATTGGAGGAAGTA+GGG | + | chr4.4:23527825-23527844 | None:intergenic | 35.0% | |
| ACAAGAGTAGTAGAATCATC+AGG | + | chr4.4:23528287-23528306 | None:intergenic | 35.0% | |
| ACGATAACTGGAAGAAACAA+TGG | - | chr4.4:23527973-23527992 | MS.gene33754:CDS | 35.0% | |
| TGAACAAGTACAACGATAAC+TGG | - | chr4.4:23527961-23527980 | MS.gene33754:CDS | 35.0% | |
| TTAAGCAACGTTGAAAAAGC+AGG | - | chr4.4:23528074-23528093 | MS.gene33754:CDS | 35.0% | |
| ! | TACTACTCTTGTTGTAGTTC+AGG | - | chr4.4:23528295-23528314 | MS.gene33754:CDS | 35.0% |
| !!! | ACTAGGACTTTTTTCTAAGG+CGG | - | chr4.4:23528148-23528167 | MS.gene33754:CDS | 35.0% |
| AGGAAGTGAAGAAGTAGAAG+TGG | - | chr4.4:23528019-23528038 | MS.gene33754:CDS | 40.0% | |
| CTCTCTTCAATCGAGAAACT+AGG | - | chr4.4:23528131-23528150 | MS.gene33754:CDS | 40.0% | |
| TAGTGTGGATGTGTTGAAGA+TGG | + | chr4.4:23527771-23527790 | None:intergenic | 40.0% | |
| TTTGCTGGATCTGTTGTTCT+CGG | - | chr4.4:23528359-23528378 | MS.gene33754:CDS | 40.0% | |
| !!! | TTTTCTAAGGCGGAAGAACT+TGG | - | chr4.4:23528158-23528177 | MS.gene33754:CDS | 40.0% |
| GACGGTGAAAGGTTTATTGG+AGG | + | chr4.4:23527833-23527852 | None:intergenic | 45.0% | |
| GGAGCAGGAATATTCTACGA+AGG | - | chr4.4:23527999-23528018 | MS.gene33754:CDS | 45.0% | |
| TAGGGAATGAGCGATAGTGA+CGG | + | chr4.4:23527807-23527826 | None:intergenic | 45.0% | |
| ! | AAGAAACAATGGTTCGGAGC+AGG | - | chr4.4:23527984-23528003 | MS.gene33754:CDS | 45.0% |
| !! | TACCTTCTTTTTGGGAGCCA+TGG | + | chr4.4:23527860-23527879 | None:intergenic | 45.0% |
| ACCTTTCACCGTCTTTGCCA+TGG | - | chr4.4:23527840-23527859 | MS.gene33754:CDS | 50.0% | |
| GACGCCGAATTCTTCAGCTT+TGG | + | chr4.4:23528108-23528127 | None:intergenic | 50.0% | |
| GTGTTGAAGATGGAGAGCTG+AGG | + | chr4.4:23527761-23527780 | None:intergenic | 50.0% | |
| TGAAGATGGAGAGCTGAGGT+TGG | + | chr4.4:23527757-23527776 | None:intergenic | 50.0% | |
| TGCCATGGCTCCCAAAAAGA+AGG | - | chr4.4:23527855-23527874 | MS.gene33754:CDS | 50.0% | |
| TTTGGGAGCCATGGCAAAGA+CGG | + | chr4.4:23527851-23527870 | None:intergenic | 50.0% | |
| ! | GAATGAGCGATAGTGACGGA+GGG | + | chr4.4:23527803-23527822 | None:intergenic | 50.0% |
| AAGCGCCAGAGAAGCCAAGA+CGG | + | chr4.4:23528225-23528244 | None:intergenic | 55.0% | |
| CAAGACGGAAGGAGAGACTG+CGG | + | chr4.4:23528210-23528229 | None:intergenic | 55.0% | |
| CTGGATCTGTTGTTCTCGGC+GGG | - | chr4.4:23528363-23528382 | MS.gene33754:CDS | 55.0% | |
| GCCATGGCAAAGACGGTGAA+AGG | + | chr4.4:23527844-23527863 | None:intergenic | 55.0% | |
| GCTGGATCTGTTGTTCTCGG+CGG | - | chr4.4:23528362-23528381 | MS.gene33754:CDS | 55.0% | |
| GGAATGAGCGATAGTGACGG+AGG | + | chr4.4:23527804-23527823 | None:intergenic | 55.0% | |
| TCCTTCCGTCTTGGCTTCTC+TGG | - | chr4.4:23528217-23528236 | MS.gene33754:intron | 55.0% | |
| ! | GCAACAGCAGCCAAAAGCGT+CGG | + | chr4.4:23528248-23528267 | None:intergenic | 55.0% |
| !! | CTCTTGGTGTTGGGGCTGTT+GGG | - | chr4.4:23528333-23528352 | MS.gene33754:CDS | 55.0% |
| !! | GCTTTTGGCTGCTGTTGCGA+CGG | - | chr4.4:23528250-23528269 | MS.gene33754:intron | 55.0% |
| !! | TCGCTGCTGCTCTTGGTGTT+GGG | - | chr4.4:23528324-23528343 | MS.gene33754:CDS | 55.0% |
| CGCAGTCTCTCCTTCCGTCT+TGG | - | chr4.4:23528208-23528227 | MS.gene33754:intron | 60.0% | |
| GCCAGAGAAGCCAAGACGGA+AGG | + | chr4.4:23528221-23528240 | None:intergenic | 60.0% | |
| GCTGTTGTCGCTGCTGCTCT+TGG | - | chr4.4:23528317-23528336 | MS.gene33754:CDS | 60.0% | |
| TGGAGAGCTGAGGTTGGAGC+AGG | + | chr4.4:23527751-23527770 | None:intergenic | 60.0% | |
| TTTGGCTGCTGTTGCGACGG+TGG | - | chr4.4:23528253-23528272 | MS.gene33754:intron | 60.0% | |
| ! | AGCGATAGTGACGGAGGGTG+CGG | + | chr4.4:23527798-23527817 | None:intergenic | 60.0% |
| ! | GGGGCTGTTGGGTTGTTTGC+TGG | - | chr4.4:23528344-23528363 | MS.gene33754:CDS | 60.0% |
| ! | GTCGCTGCTGCTCTTGGTGT+TGG | - | chr4.4:23528323-23528342 | MS.gene33754:CDS | 60.0% |
| ! | TCTGGCGCTTCCGACGCTTT+TGG | - | chr4.4:23528235-23528254 | MS.gene33754:intron | 60.0% |
| !! | CGCTGCTGCTCTTGGTGTTG+GGG | - | chr4.4:23528325-23528344 | MS.gene33754:CDS | 60.0% |
| !! | GCTCTTGGTGTTGGGGCTGT+TGG | - | chr4.4:23528332-23528351 | MS.gene33754:CDS | 60.0% |
| !! | TGTTGTTCTCGGCGGGTTGC+AGG | - | chr4.4:23528370-23528389 | MS.gene33754:CDS | 60.0% |
| ! | GATAGTGACGGAGGGTGCGG+TGG | + | chr4.4:23527795-23527814 | None:intergenic | 65.0% |
| ! | GGAGGGTGCGGTGGTTAGTG+TGG | + | chr4.4:23527786-23527805 | None:intergenic | 65.0% |
| !! | TGTTCTCGGCGGGTTGCAGG+AGG | - | chr4.4:23528373-23528392 | MS.gene33754:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 23527739 | 23528403 | 23527739 | ID=MS.gene33754 |
| chr4.4 | mRNA | 23527739 | 23528403 | 23527739 | ID=MS.gene33754.t1;Parent=MS.gene33754 |
| chr4.4 | exon | 23528266 | 23528403 | 23528266 | ID=MS.gene33754.t1.exon1;Parent=MS.gene33754.t1 |
| chr4.4 | CDS | 23528266 | 23528403 | 23528266 | ID=cds.MS.gene33754.t1;Parent=MS.gene33754.t1 |
| chr4.4 | exon | 23527739 | 23528182 | 23527739 | ID=MS.gene33754.t1.exon2;Parent=MS.gene33754.t1 |
| chr4.4 | CDS | 23527739 | 23528182 | 23527739 | ID=cds.MS.gene33754.t1;Parent=MS.gene33754.t1 |
| Gene Sequence |
| Protein sequence |
