AlfalfaGEDB | Msa0311730

M. sativa cultivar zhongmu-4 / Msa0311730

KEGG: -
GO: -
PFAM: PF04749.20

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0311730	XP_013462453.1	99.190	247	2	0	1	247	1	247	0.0	508

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0311730	sp\|Q9M815\|PCR8_ARATH	29.655	145	70	7	71	210	48	165	4.69e-11	63.5

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0011930	Msa0311730	0.818244	2.168657e-52	-8.615850e-47
Msa0020390	Msa0311730	0.822012	2.974762e-53	-8.615850e-47
Msa0031990	Msa0311730	-0.800046	1.731904e-48	-8.615850e-47
Msa0032860	Msa0311730	0.800366	1.490953e-48	-8.615850e-47
Msa0038870	Msa0311730	0.807534	4.824156e-50	-8.615850e-47
Msa0065680	Msa0311730	-0.813082	3.064993e-51	-8.615850e-47
Msa0086420	Msa0311730	0.802673	5.017479e-49	-8.615850e-47
Msa0090570	Msa0311730	0.816126	6.494573e-52	-8.615850e-47
Msa0106620	Msa0311730	0.807933	3.968140e-50	-8.615850e-47
Msa0106710	Msa0311730	0.815470	9.093321e-52	-8.615850e-47
Msa0108390	Msa0311730	0.804996	1.651704e-49	-8.615850e-47
Msa0127800	Msa0311730	0.801019	1.096889e-48	-8.615850e-47
Msa0130720	Msa0311730	0.808581	2.886338e-50	-8.615850e-47
Msa0139670	Msa0311730	0.826644	2.422986e-54	-8.615850e-47
Msa0159420	Msa0311730	0.833971	3.924935e-56	-8.615850e-47
Msa0167980	Msa0311730	0.810427	1.158860e-50	-8.615850e-47
Msa0183850	Msa0311730	0.833662	4.689737e-56	-8.615850e-47
Msa0192640	Msa0311730	0.811470	6.888455e-51	-8.615850e-47
Msa0204960	Msa0311730	0.838467	2.827260e-57	-8.615850e-47
Msa0206690	Msa0311730	0.804678	1.924720e-49	-8.615850e-47
Msa0209500	Msa0311730	0.801642	8.178844e-49	-8.615850e-47
Msa0217670	Msa0311730	0.823711	1.195621e-53	-8.615850e-47
Msa0223330	Msa0311730	0.822178	2.722527e-53	-8.615850e-47
Msa0233380	Msa0311730	0.879987	7.953308e-70	-8.615850e-47
Msa0241830	Msa0311730	0.802931	4.439179e-49	-8.615850e-47
Msa0257490	Msa0311730	0.845897	3.048913e-59	-8.615850e-47
Msa0259210	Msa0311730	0.839486	1.539254e-57	-8.615850e-47
Msa0271360	Msa0311730	0.814944	1.190434e-51	-8.615850e-47
Msa0281530	Msa0311730	0.828701	7.765925e-55	-8.615850e-47
Msa0283420	Msa0311730	0.803730	3.031513e-49	-8.615850e-47
Msa0288080	Msa0311730	0.805330	1.406273e-49	-8.615850e-47
Msa0296790	Msa0311730	0.869414	3.152077e-66	-8.615850e-47
Msa0296820	Msa0311730	0.804132	2.502010e-49	-8.615850e-47
Msa0311060	Msa0311730	0.806481	8.055654e-50	-8.615850e-47
Msa0311720	Msa0311730	0.827854	1.242878e-54	-8.615850e-47
Msa0311730	Msa0312910	0.809132	2.200578e-50	-8.615850e-47
Msa0311730	Msa0317970	0.822932	1.817926e-53	-8.615850e-47
Msa0311730	Msa0320590	0.803960	2.715888e-49	-8.615850e-47
Msa0311730	Msa0322900	0.801906	7.217711e-49	-8.615850e-47
Msa0311730	Msa0326480	0.803213	3.881129e-49	-8.615850e-47
Msa0311730	Msa0336310	0.820886	5.412420e-53	-8.615850e-47
Msa0311730	Msa0336320	0.840670	7.562179e-58	-8.615850e-47
Msa0311730	Msa0339910	0.823311	1.483444e-53	-8.615850e-47
Msa0311730	Msa0343850	0.821753	3.414548e-53	-8.615850e-47
Msa0311730	Msa0381610	-0.800868	1.177854e-48	-8.615850e-47
Msa0311730	Msa0382370	0.838700	2.461505e-57	-8.615850e-47
Msa0311730	Msa0385500	0.820333	7.249788e-53	-8.615850e-47
Msa0311730	Msa0395980	0.808447	3.082989e-50	-8.615850e-47
Msa0311730	Msa0403770	0.824655	7.175662e-54	-8.615850e-47
Msa0311730	Msa0432160	0.834019	3.818131e-56	-8.615850e-47
Msa0311730	Msa0450400	-0.828282	9.801937e-55	-8.615850e-47
Msa0311730	Msa0469620	-0.803785	2.954031e-49	-8.615850e-47
Msa0311730	Msa0476680	0.835128	2.009778e-56	-8.615850e-47
Msa0311730	Msa0491000	0.823794	1.143586e-53	-8.615850e-47
Msa0311730	Msa0511770	0.826790	2.235509e-54	-8.615850e-47
Msa0311730	Msa0521010	0.840180	1.015832e-57	-8.615850e-47
Msa0311730	Msa0542290	0.814446	1.534680e-51	-8.615850e-47
Msa0311730	Msa0542560	0.806109	9.649050e-50	-8.615850e-47
Msa0311730	Msa0547160	0.804191	2.432036e-49	-8.615850e-47
Msa0311730	Msa0547990	0.802599	5.196188e-49	-8.615850e-47
Msa0311730	Msa0567240	0.810843	9.423903e-51	-8.615850e-47
Msa0311730	Msa0574390	0.804628	1.971914e-49	-8.615850e-47
Msa0311730	Msa0600080	0.808496	3.010016e-50	-8.615850e-47
Msa0311730	Msa0600450	0.821736	3.446393e-53	-8.615850e-47
Msa0311730	Msa0604290	0.808074	3.703108e-50	-8.615850e-47
Msa0311730	Msa0629240	0.812704	3.707729e-51	-8.615850e-47
Msa0311730	Msa0640940	0.823113	1.649808e-53	-8.615850e-47
Msa0311730	Msa0642970	0.803335	3.661536e-49	-8.615850e-47
Msa0311730	Msa0644030	0.801292	9.645167e-49	-8.615850e-47
Msa0311730	Msa0679360	0.829546	4.844989e-55	-8.615850e-47
Msa0311730	Msa0682680	0.808336	3.255469e-50	-8.615850e-47
Msa0311730	Msa0697720	0.801320	9.520004e-49	-8.615850e-47
Msa0311730	Msa0709120	0.824074	9.829891e-54	-8.615850e-47
Msa0311730	Msa0721280	0.808611	2.844698e-50	-8.615850e-47
Msa0311730	Msa0746170	0.802056	6.723529e-49	-8.615850e-47
Msa0311730	Msa0746210	0.805110	1.563941e-49	-8.615850e-47
Msa0311730	Msa0747260	0.802901	4.501593e-49	-8.615850e-47
Msa0311730	Msa0748920	0.810250	1.265679e-50	-8.615850e-47
Msa0311730	Msa0750250	0.808165	3.540934e-50	-8.615850e-47
Msa0311730	Msa0763890	0.800415	1.456923e-48	-8.615850e-47
Msa0311730	Msa0763920	0.809869	1.528574e-50	-8.615850e-47
Msa0311730	Msa0767100	0.817335	3.478184e-52	-8.615850e-47
Msa0311730	Msa0789560	0.814730	1.327321e-51	-8.615850e-47
Msa0311730	Msa0826150	0.803101	4.092802e-49	-8.615850e-47
Msa0311730	Msa0861500	0.806731	7.135541e-50	-8.615850e-47
Msa0311730	Msa0890550	0.812849	3.446927e-51	-8.615850e-47
Msa0311730	Msa0892640	0.804697	1.907927e-49	-8.615850e-47
Msa0311730	Msa0894710	0.811130	8.166334e-51	-8.615850e-47
Msa0311730	Msa0897200	0.828955	6.740079e-55	-8.615850e-47
Msa0311730	Msa0921420	0.801035	1.088549e-48	-8.615850e-47
Msa0311730	Msa0923780	0.835161	1.971198e-56	-8.615850e-47
Msa0311730	Msa0944200	0.827321	1.669060e-54	-8.615850e-47
Msa0311730	Msa0962840	0.847707	9.750298e-60	-8.615850e-47
Msa0311730	Msa0969370	0.813027	3.150972e-51	-8.615850e-47
Msa0311730	Msa0970990	0.803153	3.993096e-49	-8.615850e-47
Msa0311730	Msa0973320	0.806879	6.639763e-50	-8.615850e-47
Msa0311730	Msa0974950	0.804147	2.483957e-49	-8.615850e-47
Msa0311730	Msa0994370	0.826613	2.463878e-54	-8.615850e-47
Msa0311730	Msa1000930	0.819515	1.115513e-52	-8.615850e-47
Msa0311730	Msa1006010	-0.803224	3.860218e-49	-8.615850e-47
Msa0311730	Msa1011090	0.806379	8.464059e-50	-8.615850e-47
Msa0311730	Msa1036610	0.812952	3.272730e-51	-8.615850e-47
Msa0311730	Msa1037520	0.816504	5.346087e-52	-8.615850e-47
Msa0311730	Msa1063420	0.802677	5.009041e-49	-8.615850e-47
Msa0311730	Msa1068870	0.807299	5.408858e-50	-8.615850e-47
Msa0311730	Msa1072900	0.831602	1.520690e-55	-8.615850e-47
Msa0311730	Msa1089450	0.806312	8.745414e-50	-8.615850e-47
Msa0311730	Msa1125790	-0.802337	5.885783e-49	-8.615850e-47
Msa0311730	Msa1131490	0.826483	2.646348e-54	-8.615850e-47
Msa0311730	Msa1177780	0.800804	1.213607e-48	-8.615850e-47
Msa0311730	Msa1206270	0.815581	8.591133e-52	-8.615850e-47
Msa0311730	Msa1206290	0.819227	1.297102e-52	-8.615850e-47
Msa0311730	Msa1208790	0.805067	1.596281e-49	-8.615850e-47
Msa0311730	Msa1232740	0.805042	1.615353e-49	-8.615850e-47
Msa0311730	Msa1234620	0.804256	2.356869e-49	-8.615850e-47
Msa0311730	Msa1265280	0.803746	3.009690e-49	-8.615850e-47
Msa0311730	Msa1271130	0.855230	7.250646e-62	-8.615850e-47
Msa0311730	Msa1272620	0.803822	2.901288e-49	-8.615850e-47
Msa0311730	Msa1276970	0.818596	1.804718e-52	-8.615850e-47
Msa0311730	Msa1310400	0.802827	4.663157e-49	-8.615850e-47
Msa0311730	Msa1313320	0.802432	5.626434e-49	-8.615850e-47
Msa0311730	Msa1332050	0.805581	1.245846e-49	-8.615850e-47
Msa0311730	Msa1337480	0.814895	1.220233e-51	-8.615850e-47
Msa0311730	Msa1357890	0.809940	1.475578e-50	-8.615850e-47
Msa0311730	Msa1359270	0.909583	4.848909e-82	-8.615850e-47
Msa0311730	Msa1365800	0.807659	4.536622e-50	-8.615850e-47
Msa0311730	Msa1368530	0.804692	1.912200e-49	-8.615850e-47
Msa0311730	Msa1390700	0.827891	1.218086e-54	-8.615850e-47
Msa0311730	Msa1392910	0.835589	1.537168e-56	-8.615850e-47
Msa0311730	Msa1398090	0.813500	2.480650e-51	-8.615850e-47
Msa0311730	Msa1407190	0.802153	6.421924e-49	-8.615850e-47
Msa0311730	Msa1424350	0.806060	9.882902e-50	-8.615850e-47
Msa0311730	Msa1458090	0.815231	1.027863e-51	-8.615850e-47
Msa0311730	Msa1458610	0.830544	2.767197e-55	-8.615850e-47
Msa0311730	Msa1460320	-0.815216	1.035508e-51	-8.615850e-47
Msa0311730	Msa1465570	0.820276	7.470004e-53	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0311730	MtrunA17_Chr2g0280931	99.190	247	2	0	1	247	1	247	0.0	508
Msa0311730	MtrunA17_Chr4g0048561	69.340	212	65	0	33	244	31	242	9.25e-106	306
Msa0311730	MtrunA17_Chr8g0356161	87.879	33	4	0	96	128	12	44	5.33e-14	65.1
Msa0311730	MtrunA17_Chr2g0331531	28.571	140	68	6	76	210	33	145	2.36e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0311730	AT1G11380.1	58.252	206	83	3	31	235	41	244	2.22e-86	257
Msa0311730	AT1G52200.1	29.655	145	70	7	71	210	48	165	4.76e-12	63.5
Msa0311730	AT1G52200.2	30.323	155	75	7	71	219	48	175	1.52e-11	62.0
Msa0311730	AT5G05350.1	34.091	132	77	3	76	197	307	438	2.10e-11	63.9

Find 101 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTACTGGTGGAGCATATTT+AGG	0.217317	2_4:+6622498	Msa0311730:CDS
TTTGGAGGGTATAGCTGTTT+TGG	0.232920	2_4:+6620841	Msa0311730:CDS
TCTTGGTTCTTGCTATATTC+AGG	0.268571	2_4:+6622195	Msa0311730:CDS
TATGCTCTATTTCTTCTATA+AGG	0.282864	2_4:-6623409	None:intergenic
AATACAAGTGCAAAGGAAAT+AGG	0.296607	2_4:-6624058	None:intergenic
TGAAGAGAATGAGAGGCTTT+TGG	0.304843	2_4:+6620823	Msa0311730:CDS
AGTTGTCCCTGCTACCGATT+TGG	0.306191	2_4:+6622151	Msa0311730:intron
ATTCATCTTAACTCTCTTGT+AGG	0.309483	2_4:-6624006	None:intergenic
GAAATCTAAGACAACCCTTT+TGG	0.313872	2_4:-6620655	None:intergenic
AGTATGTCATTCTAAACTAA+TGG	0.321468	2_4:+6623861	Msa0311730:three_prime_UTR
GAAAATAAGTCTACACTGTT+TGG	0.329716	2_4:+6623624	Msa0311730:three_prime_UTR
GAATTGCTCTTGAATCTTCA+TGG	0.338376	2_4:+6621020	Msa0311730:CDS
ATCAAGCTTGGCTCTATTAT+AGG	0.343228	2_4:+6624027	Msa0311730:three_prime_UTR
TTAGAATGACATACTCTATA+TGG	0.350268	2_4:-6623853	None:intergenic
TACAAACTGCAAATGGAACT+TGG	0.350486	2_4:+6620894	Msa0311730:CDS
ACAGTCACCTTCATCATTAC+TGG	0.358307	2_4:+6622483	Msa0311730:CDS
TACAGCAAGGACAGACAAAA+TGG	0.360421	2_4:-6623046	None:intergenic
GACCAAAGTGCATGTAAGTT+TGG	0.366129	2_4:-6623724	None:intergenic
GTAGGCCTACTTGAGAATAA+AGG	0.372321	2_4:-6623988	None:intergenic
GTTGTCCCTGCTACCGATTT+GGG	0.374348	2_4:+6622152	Msa0311730:intron
GTATGTCATTCTAAACTAAT+GGG	0.376856	2_4:+6623862	Msa0311730:three_prime_UTR
AGATGGCACTTGGCACGGTC+GGG	0.384709	2_4:+6623284	Msa0311730:CDS
GTTGCTTTACAAACTGCAAA+TGG	0.416678	2_4:+6620887	Msa0311730:CDS
CCATATGCATAGGTGGCATT+AGG	0.420138	2_4:+6623313	Msa0311730:CDS
AACATGAAGCGAGCTGGTCT+TGG	0.436073	2_4:+6622178	Msa0311730:CDS
CCTAATGCCACCTATGCATA+TGG	0.438749	2_4:-6623313	None:intergenic
GGGTTGTCTTAGATTTCAAA+AGG	0.443283	2_4:+6620661	Msa0311730:five_prime_UTR
ACCCATTAAGCAAAACCAAA+AGG	0.447938	2_4:+6620640	Msa0311730:five_prime_UTR
CTAATCAAACTATATACTAC+AGG	0.453185	2_4:-6623903	None:intergenic
TGTTGTTGTTGGTGGGGTGT+AGG	0.458669	2_4:-6620579	None:intergenic
GTGATGTCTCGTGACGATAA+AGG	0.459117	2_4:-6622445	None:intergenic
ACAAACTGCAAATGGAACTT+GGG	0.459731	2_4:+6620895	Msa0311730:CDS
ATTTCTTTCGCATACGAGTT+CGG	0.461273	2_4:-6622527	None:intergenic
GTTCTTCCCAAATCGGTAGC+AGG	0.463827	2_4:-6622158	None:intergenic
TTTCATCTGAATTGTATCCC+TGG	0.470447	2_4:+6623486	Msa0311730:three_prime_UTR
GCTTCATGTTCTTCCCAAAT+CGG	0.471525	2_4:-6622165	None:intergenic
AAGATGGCACTTGGCACGGT+CGG	0.472962	2_4:+6623283	Msa0311730:CDS
TAATGATGAAGGTGACTGTC+AGG	0.473340	2_4:-6622479	None:intergenic
TCCTTGCTGTACATTATGTC+AGG	0.477662	2_4:+6623058	Msa0311730:CDS
AAATATGCCGGGCAGACACC+AGG	0.483030	2_4:-6623504	None:intergenic
TTAATCCTTTATTCTCAAGT+AGG	0.488913	2_4:+6623983	Msa0311730:three_prime_UTR
GAAACAACTAATGTTTGTGT+TGG	0.490041	2_4:+6623602	Msa0311730:three_prime_UTR
AAACTATATACTACAGGGGC+TGG	0.491204	2_4:-6623897	None:intergenic
GAACAGCGTGGTGAGGAGTT+TGG	0.502636	2_4:+6620944	Msa0311730:CDS
TGCATAGGTGGCATTAGGAA+TGG	0.509331	2_4:+6623318	Msa0311730:CDS
ATATTGAGAATAAGTCTACT+TGG	0.509797	2_4:-6623756	None:intergenic
TATCGAGTCGATCAAGCTTA+CGG	0.510820	2_4:+6623374	Msa0311730:CDS
TTCCAAACTTACATGCACTT+TGG	0.511346	2_4:+6623722	Msa0311730:three_prime_UTR
GATGGCACTTGGCACGGTCG+GGG	0.514885	2_4:+6623285	Msa0311730:CDS
CCCGGTACGATCGGTGCTGT+TGG	0.518805	2_4:-6623555	None:intergenic
CCCATTAAGCAAAACCAAAA+GGG	0.526690	2_4:+6620641	Msa0311730:five_prime_UTR
AAGGTGATGGTGATGGATGA+AGG	0.527235	2_4:+6620785	Msa0311730:CDS
TTTGTTACAATCATTAAGAA+GGG	0.528792	2_4:-6623944	None:intergenic
CTAAGAGGTAGTGGCAATCA+CGG	0.529900	2_4:+6623579	Msa0311730:three_prime_UTR
GCATAGGTGGCATTAGGAAT+GGG	0.533084	2_4:+6623319	Msa0311730:CDS
AGCTTGATCGACTCGATAGG+AGG	0.534493	2_4:-6623369	None:intergenic
GATGAAGGAGAAGGAAGAGA+AGG	0.534692	2_4:+6620800	Msa0311730:CDS
CCGATCGTACCGGGACTAAG+AGG	0.537306	2_4:+6623564	Msa0311730:three_prime_UTR
GAGAAGGTGAAGAGAATGAG+AGG	0.541850	2_4:+6620816	Msa0311730:CDS
GCCAACAGCACCGATCGTAC+CGG	0.543319	2_4:+6623554	Msa0311730:three_prime_UTR
GAAGTTGAAAAGAGCACAGA+TGG	0.544266	2_4:-6622418	None:intergenic
TTGTATCCCTGGTGTCTGCC+CGG	0.545230	2_4:+6623497	Msa0311730:three_prime_UTR
ACAACGTTCAAGATGGCACT+TGG	0.547096	2_4:+6623274	Msa0311730:CDS
CCAACAGCACCGATCGTACC+GGG	0.551891	2_4:+6623555	Msa0311730:three_prime_UTR
CCTCTTAGTCCCGGTACGAT+CGG	0.555815	2_4:-6623564	None:intergenic
TGCTCCACCAGTAATGATGA+AGG	0.556716	2_4:-6622490	None:intergenic
GCGAAAGAAATTCAACATCA+AGG	0.559237	2_4:+6622539	Msa0311730:CDS
GGGAAGAACATGAAGCGAGC+TGG	0.560543	2_4:+6622172	Msa0311730:CDS
AAGCACAAGTGGGAAGATGT+TGG	0.565703	2_4:-6620541	None:intergenic
GGTTGTCTTAGATTTCAAAA+GGG	0.568294	2_4:+6620662	Msa0311730:five_prime_UTR
GAGATTAACAACGTTCAAGA+TGG	0.577162	2_4:+6623267	Msa0311730:CDS
TTCTTCCCAAATCGGTAGCA+GGG	0.579813	2_4:-6622157	None:intergenic
GTACCGGGACTAAGAGGTAG+TGG	0.587925	2_4:+6623570	Msa0311730:three_prime_UTR
CAAACTGCAAATGGAACTTG+GGG	0.589199	2_4:+6620896	Msa0311730:CDS
TTTGGAGAAGCAACAAGAGA+AGG	0.590249	2_4:+6620766	Msa0311730:CDS
TAAGAGGTAGTGGCAATCAC+GGG	0.592065	2_4:+6623580	Msa0311730:three_prime_UTR
CGGGGTGACACCATATGCAT+AGG	0.592251	2_4:+6623303	Msa0311730:CDS
TAATCAAACTATATACTACA+GGG	0.601716	2_4:-6623902	None:intergenic
GTAAGCTTGATCGACTCGAT+AGG	0.604079	2_4:-6623372	None:intergenic
GAGTTAAGATGAATCAAGCT+TGG	0.612315	2_4:+6624015	Msa0311730:three_prime_UTR
AATCTTGAATCAAAGTTGTG+AGG	0.612660	2_4:+6623444	Msa0311730:three_prime_UTR
TTGATCGACTCGATAGGAGG+AGG	0.612757	2_4:-6623366	None:intergenic
ACAAGAGAAGGTGATGGTGA+TGG	0.614123	2_4:+6620778	Msa0311730:CDS
CTATTGAAAGCAAGCACAAG+TGG	0.616688	2_4:-6620552	None:intergenic
GTCACCTTCATCATTACTGG+TGG	0.618594	2_4:+6622486	Msa0311730:CDS
GAAGCAACAAGAGAAGGTGA+TGG	0.622784	2_4:+6620772	Msa0311730:CDS
ACTTGTTAATACAAGTGCAA+AGG	0.635681	2_4:-6624065	None:intergenic
GTTCAAGATGGCACTTGGCA+CGG	0.636982	2_4:+6623279	Msa0311730:CDS
TCAGGGTAGCGACAGTATGG+TGG	0.637621	2_4:+6623010	Msa0311730:intron
ACCTGACATAATGTACAGCA+AGG	0.643383	2_4:-6623059	None:intergenic
TATTGAAAGCAAGCACAAGT+GGG	0.643837	2_4:-6620551	None:intergenic
GGTGACACCATATGCATAGG+TGG	0.644206	2_4:+6623306	Msa0311730:CDS
TTGCCACTACCTCTTAGTCC+CGG	0.648542	2_4:-6623573	None:intergenic
TATGTCATTCTAAACTAATG+GGG	0.654822	2_4:+6623863	Msa0311730:three_prime_UTR
CAGCGTGGTGAGGAGTTTGG+TGG	0.655669	2_4:+6620947	Msa0311730:CDS
AATATGCCGGGCAGACACCA+GGG	0.660338	2_4:-6623503	None:intergenic
AATCAAACTATATACTACAG+GGG	0.676678	2_4:-6623901	None:intergenic
ATGGTGATGGATGAAGGAGA+AGG	0.692827	2_4:+6620791	Msa0311730:CDS
AGATGATGAACAGCGTGGTG+AGG	0.702639	2_4:+6620937	Msa0311730:CDS
AAACTGCAAATGGAACTTGG+GGG	0.738736	2_4:+6620897	Msa0311730:CDS
GGTGGAGATGATGAACAGCG+TGG	0.824994	2_4:+6620932	Msa0311730:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAACATTTTAACTTTAAATT+CGG	-	chr2_4:6621677-6621696	None:intergenic	10.0%
!!	TATGTTTAAGAAAACAAAAA+AGG	-	chr2_4:6621065-6621084	None:intergenic	15.0%
!!!	TTAATAAGTTTTAGAATGAA+TGG	+	chr2_4:6621470-6621489	Msa0311730:intron	15.0%
!!!	TAGCAACTAAAATTTAATTT+TGG	+	chr2_4:6622748-6622767	Msa0311730:intron	15.0%
!!	AAAATTCAATACAATAATGT+TGG	-	chr2_4:6623209-6623228	None:intergenic	15.0%
!!!	AAGTTGGTAACTTTTTTTTT+TGG	+	chr2_4:6620724-6620743	Msa0311730:five_prime_UTR	20.0%
!!!	AGTTGGTAACTTTTTTTTTT+GGG	+	chr2_4:6620725-6620744	Msa0311730:five_prime_UTR	20.0%
!!!	TTTTGTTTTTGTTCATTTTG+AGG	+	chr2_4:6621142-6621161	Msa0311730:intron	20.0%
!!!	CTATTTTTGAGATAGAATTT+TGG	-	chr2_4:6621440-6621459	None:intergenic	20.0%
!!	TATATTGGGACATTAATTTA+AGG	-	chr2_4:6621627-6621646	None:intergenic	20.0%
!!	GAAAATAACCAAGATATATT+GGG	-	chr2_4:6621641-6621660	None:intergenic	20.0%
!!	TGAAAATAACCAAGATATAT+TGG	-	chr2_4:6621642-6621661	None:intergenic	20.0%
!!!	AATATATCTTGGTTATTTTC+AGG	+	chr2_4:6621641-6621660	Msa0311730:intron	20.0%
!!!	TTTTGTTAAAGCTTTAAGTT+AGG	-	chr2_4:6622279-6622298	None:intergenic	20.0%
!!!	TAACTTAAAGCTTTAACAAA+AGG	+	chr2_4:6622278-6622297	Msa0311730:intron	20.0%
!!	TGAACAAGACAAATTATATT+TGG	-	chr2_4:6622656-6622675	None:intergenic	20.0%
!!!	TGTGCTTTAAATTAAATCAA+GGG	-	chr2_4:6622719-6622738	None:intergenic	20.0%
!!!	TTGTGCTTTAAATTAAATCA+AGG	-	chr2_4:6622720-6622739	None:intergenic	20.0%
!!	ATAGTTGAACTAAATTAGAA+AGG	-	chr2_4:6623158-6623177	None:intergenic	20.0%
!!!	TAATAAATATGCTCTCATTT+TGG	-	chr2_4:6623183-6623202	None:intergenic	20.0%
!!!	TTTTTTGAAATCAAATATGC+CGG	-	chr2_4:6623519-6623538	None:intergenic	20.0%
!!	TTCAAAAAAGAATGTAATGA+AGG	+	chr2_4:6623530-6623549	Msa0311730:three_prime_UTR	20.0%
!!	TAATCAAACTATATACTACA+GGG	-	chr2_4:6623905-6623924	None:intergenic	20.0%
!!	TTTGTTACAATCATTAAGAA+GGG	-	chr2_4:6623947-6623966	None:intergenic	20.0%
!!!	TTTTGTTACAATCATTAAGA+AGG	-	chr2_4:6623948-6623967	None:intergenic	20.0%
!!!	ACTTTTTTTTTTGGGTTTTG+AGG	+	chr2_4:6620733-6620752	Msa0311730:five_prime_UTR	25.0%
!!!	TCATTTTGAGGTTTTTTGTT+TGG	+	chr2_4:6621154-6621173	Msa0311730:intron	25.0%
!	AAACATAACAAGCAAACAAT+TGG	-	chr2_4:6621278-6621297	None:intergenic	25.0%
!	ATCACAAAAGAATATCTTAC+AGG	-	chr2_4:6621363-6621382	None:intergenic	25.0%
!	AAAGTCTAAACATAGGATTA+AGG	-	chr2_4:6621394-6621413	None:intergenic	25.0%
!	TTAATCCTATGTTTAGACTT+TGG	+	chr2_4:6621393-6621412	Msa0311730:intron	25.0%
!	ATTAATGTCCCAATATATCT+TGG	+	chr2_4:6621630-6621649	Msa0311730:intron	25.0%
!	AAAATCAGAGAATTTGAACA+TGG	-	chr2_4:6622328-6622347	None:intergenic	25.0%
!!!	ATATCCTTGTTTTATATTGC+AGG	+	chr2_4:6622374-6622393	Msa0311730:intron	25.0%
!!	TAATCTAAAGAGTGCATATT+AGG	+	chr2_4:6622684-6622703	Msa0311730:intron	25.0%
!!	AATCTAAAGAGTGCATATTA+GGG	+	chr2_4:6622685-6622704	Msa0311730:intron	25.0%
!	ATATCTAATGCAGTATATGT+TGG	+	chr2_4:6622978-6622997	Msa0311730:intron	25.0%
!!	ATATGTTGGTATCTATTTTC+AGG	+	chr2_4:6622992-6623011	Msa0311730:intron	25.0%
!!	TATGTTGGTATCTATTTTCA+GGG	+	chr2_4:6622993-6623012	Msa0311730:intron	25.0%
!!	TATGCTCTATTTCTTCTATA+AGG	-	chr2_4:6623412-6623431	None:intergenic	25.0%
!!!	TTTTTGAAATCAAATATGCC+GGG	-	chr2_4:6623518-6623537	None:intergenic	25.0%
!	ATATTGAGAATAAGTCTACT+TGG	-	chr2_4:6623759-6623778	None:intergenic	25.0%
!!!	ATATGAGATAGCTTAGTTTT+TGG	+	chr2_4:6623775-6623794	Msa0311730:three_prime_UTR	25.0%
!!	ATTTTGCAAACTAACAACAT+GGG	-	chr2_4:6623831-6623850	None:intergenic	25.0%
!	TTAGAATGACATACTCTATA+TGG	-	chr2_4:6623856-6623875	None:intergenic	25.0%
!	AGTATGTCATTCTAAACTAA+TGG	+	chr2_4:6623861-6623880	Msa0311730:three_prime_UTR	25.0%
!	GTATGTCATTCTAAACTAAT+GGG	+	chr2_4:6623862-6623881	Msa0311730:three_prime_UTR	25.0%
!	TATGTCATTCTAAACTAATG+GGG	+	chr2_4:6623863-6623882	Msa0311730:three_prime_UTR	25.0%
!	AATCAAACTATATACTACAG+GGG	-	chr2_4:6623904-6623923	None:intergenic	25.0%
!	CTAATCAAACTATATACTAC+AGG	-	chr2_4:6623906-6623925	None:intergenic	25.0%
!	TTAATCCTTTATTCTCAAGT+AGG	+	chr2_4:6623983-6624002	Msa0311730:three_prime_UTR	25.0%
!!	TTTTACGTTGTTGTTGTTGT+TGG	-	chr2_4:6620593-6620612	None:intergenic	30.0%
!!!	CTTAATGGGTTTTCAGAAAA+TGG	-	chr2_4:6620630-6620649	None:intergenic	30.0%
	GGTTGTCTTAGATTTCAAAA+GGG	+	chr2_4:6620662-6620681	Msa0311730:five_prime_UTR	30.0%
	TGAGAAAGAGAAGAAAAAGT+TGG	+	chr2_4:6620708-6620727	Msa0311730:five_prime_UTR	30.0%
!!!	TTTTTTGGGTTTTGAGGTAA+TGG	+	chr2_4:6620739-6620758	Msa0311730:exon	30.0%
!!!	TTTTTGGGTTTTGAGGTAAT+GGG	+	chr2_4:6620740-6620759	Msa0311730:exon	30.0%
!!!	TGAGGTTTTTTGTTTGGTTT+TGG	+	chr2_4:6621160-6621179	Msa0311730:intron	30.0%
	GAAGTTTAGCTAATTGTGTA+AGG	+	chr2_4:6621217-6621236	Msa0311730:intron	30.0%
	TAGCTAATTGTGTAAGGAAT+TGG	+	chr2_4:6621223-6621242	Msa0311730:intron	30.0%
!!	TGCTTGTTATGTTTCATTTC+TGG	+	chr2_4:6621284-6621303	Msa0311730:intron	30.0%
	ACTGAAACTATAAGCTCAAA+GGG	-	chr2_4:6621320-6621339	None:intergenic	30.0%
	AACTGAAACTATAAGCTCAA+AGG	-	chr2_4:6621321-6621340	None:intergenic	30.0%
	TGGGACATTAATTTAAGGAT+TGG	-	chr2_4:6621622-6621641	None:intergenic	30.0%
	CATGTCATGAACAAATTCAT+AGG	-	chr2_4:6621988-6622007	None:intergenic	30.0%
	GGAAATTGTCAATTCGAAAT+AGG	-	chr2_4:6622307-6622326	None:intergenic	30.0%
	AATGCCTGCAATATAAAACA+AGG	-	chr2_4:6622381-6622400	None:intergenic	30.0%
	AAAGAAATTCAACATCAAGG+TGG	+	chr2_4:6622542-6622561	Msa0311730:intron	30.0%
	AAGAAATTCAACATCAAGGT+GGG	+	chr2_4:6622543-6622562	Msa0311730:intron	30.0%
	AAACTTGTCTGTGAATAACT+AGG	-	chr2_4:6622598-6622617	None:intergenic	30.0%
!	GCTTTAAATTAAATCAAGGG+TGG	-	chr2_4:6622716-6622735	None:intergenic	30.0%
!!	TCCTTTAAAATCCTCTAAAG+CGG	-	chr2_4:6622789-6622808	None:intergenic	30.0%
!	GACTAACTTGGATGTAATTT+TGG	+	chr2_4:6622945-6622964	Msa0311730:intron	30.0%
	TGAGATAAAGCTATTGATGA+CGG	+	chr2_4:6623128-6623147	Msa0311730:intron	30.0%
	AATCTTGAATCAAAGTTGTG+AGG	+	chr2_4:6623444-6623463	Msa0311730:three_prime_UTR	30.0%
!!!	GTGAGGAAGTTTTAAAACAA+TGG	+	chr2_4:6623461-6623480	Msa0311730:three_prime_UTR	30.0%
	GAAACAACTAATGTTTGTGT+TGG	+	chr2_4:6623602-6623621	Msa0311730:three_prime_UTR	30.0%
	GAAAATAAGTCTACACTGTT+TGG	+	chr2_4:6623624-6623643	Msa0311730:three_prime_UTR	30.0%
!	TTTTGCAAACTAACAACATG+GGG	-	chr2_4:6623830-6623849	None:intergenic	30.0%
!	CATTTTGCAAACTAACAACA+TGG	-	chr2_4:6623832-6623851	None:intergenic	30.0%
	ATTCATCTTAACTCTCTTGT+AGG	-	chr2_4:6624009-6624028	None:intergenic	30.0%
	AATACAAGTGCAAAGGAAAT+AGG	-	chr2_4:6624061-6624080	None:intergenic	30.0%
!	ACTTGTTAATACAAGTGCAA+AGG	-	chr2_4:6624068-6624087	None:intergenic	30.0%
	TATTGAAAGCAAGCACAAGT+GGG	-	chr2_4:6620554-6620573	None:intergenic	35.0%
	ACCCATTAAGCAAAACCAAA+AGG	+	chr2_4:6620640-6620659	Msa0311730:five_prime_UTR	35.0%
!!!	CCCTTTTGGTTTTGCTTAAT+GGG	-	chr2_4:6620644-6620663	None:intergenic	35.0%
	CCCATTAAGCAAAACCAAAA+GGG	+	chr2_4:6620641-6620660	Msa0311730:five_prime_UTR	35.0%
!!!	ACCCTTTTGGTTTTGCTTAA+TGG	-	chr2_4:6620645-6620664	None:intergenic	35.0%
!!	GAAATCTAAGACAACCCTTT+TGG	-	chr2_4:6620658-6620677	None:intergenic	35.0%
	GGGTTGTCTTAGATTTCAAA+AGG	+	chr2_4:6620661-6620680	Msa0311730:five_prime_UTR	35.0%
!!!	TTTTGAGGTAATGGGTGATT+TGG	+	chr2_4:6620748-6620767	Msa0311730:exon	35.0%
!	GTTGCTTTACAAACTGCAAA+TGG	+	chr2_4:6620887-6620906	Msa0311730:CDS	35.0%
	TACAAACTGCAAATGGAACT+TGG	+	chr2_4:6620894-6620913	Msa0311730:CDS	35.0%
	ACAAACTGCAAATGGAACTT+GGG	+	chr2_4:6620895-6620914	Msa0311730:CDS	35.0%
	GAATTGCTCTTGAATCTTCA+TGG	+	chr2_4:6621020-6621039	Msa0311730:CDS	35.0%
!!	TGTAAGGAATTGGTGATGAT+TGG	+	chr2_4:6621233-6621252	Msa0311730:intron	35.0%
	TCCTATGTTTAGACTTTGGA+TGG	+	chr2_4:6621397-6621416	Msa0311730:intron	35.0%
	CCTATGTTTAGACTTTGGAT+GGG	+	chr2_4:6621398-6621417	Msa0311730:intron	35.0%
	CTATGTTTAGACTTTGGATG+GGG	+	chr2_4:6621399-6621418	Msa0311730:intron	35.0%
	TATGTTTAGACTTTGGATGG+GGG	+	chr2_4:6621400-6621419	Msa0311730:intron	35.0%
	TGACCTATCATCATCAAACT+TGG	+	chr2_4:6621498-6621517	Msa0311730:intron	35.0%
	GACCTATCATCATCAAACTT+GGG	+	chr2_4:6621499-6621518	Msa0311730:intron	35.0%
	AGAGTTTCGGAGAAAAGAAA+AGG	-	chr2_4:6621705-6621724	None:intergenic	35.0%
	TATAACAGGATGGAGAGTTT+CGG	-	chr2_4:6621718-6621737	None:intergenic	35.0%
	AATCACGTTCCTCATAGATT+CGG	-	chr2_4:6621751-6621770	None:intergenic	35.0%
	AACAGTGAAAACAGAAAGTC+AGG	-	chr2_4:6621792-6621811	None:intergenic	35.0%
	TTATTGCATACAACCCCAAA+AGG	+	chr2_4:6621815-6621834	Msa0311730:intron	35.0%
	TATTGCATACAACCCCAAAA+GGG	+	chr2_4:6621816-6621835	Msa0311730:intron	35.0%
!!	GTTTATTTTCTCATCGTGGT+GGG	+	chr2_4:6621848-6621867	Msa0311730:intron	35.0%
	TCATCATTCAAAACCATACC+AGG	-	chr2_4:6621917-6621936	None:intergenic	35.0%
	CATGACATGAACTTTCTTAC+TGG	+	chr2_4:6621999-6622018	Msa0311730:intron	35.0%
!!!	ATGCCATTTTAGACTTTCGT+TGG	-	chr2_4:6622025-6622044	None:intergenic	35.0%
	TCCAAAAAAAAGACACACAC+TGG	+	chr2_4:6622062-6622081	Msa0311730:intron	35.0%
!!!	ACCAGTGTGTGTCTTTTTTT+TGG	-	chr2_4:6622066-6622085	None:intergenic	35.0%
!!	TGGTTTGCTGTTACTTACAA+GGG	+	chr2_4:6622082-6622101	Msa0311730:intron	35.0%
!	TCTTGGTTCTTGCTATATTC+AGG	+	chr2_4:6622195-6622214	Msa0311730:CDS	35.0%
	ATTACTGGTGGAGCATATTT+AGG	+	chr2_4:6622498-6622517	Msa0311730:CDS	35.0%
	ATTTCTTTCGCATACGAGTT+CGG	-	chr2_4:6622530-6622549	None:intergenic	35.0%
	GCGAAAGAAATTCAACATCA+AGG	+	chr2_4:6622539-6622558	Msa0311730:CDS	35.0%
!!	CCTTTAAAATCCTCTAAAGC+GGG	-	chr2_4:6622788-6622807	None:intergenic	35.0%
	ATTTGTCCAAAGTTGCACTT+TGG	+	chr2_4:6622808-6622827	Msa0311730:intron	35.0%
!	GGAGTATGGTTGGTTAATAT+TGG	+	chr2_4:6622892-6622911	Msa0311730:intron	35.0%
!	TCAATACATGCTGACTAACT+TGG	+	chr2_4:6622933-6622952	Msa0311730:intron	35.0%
	AGTGTACAACAAGTCAAGAA+AGG	-	chr2_4:6623100-6623119	None:intergenic	35.0%
	GAGATTAACAACGTTCAAGA+TGG	+	chr2_4:6623267-6623286	Msa0311730:CDS	35.0%
	TTTCATCTGAATTGTATCCC+TGG	+	chr2_4:6623486-6623505	Msa0311730:three_prime_UTR	35.0%
	TTCCAAACTTACATGCACTT+TGG	+	chr2_4:6623722-6623741	Msa0311730:three_prime_UTR	35.0%
!!	GAGATAGCTTAGTTTTTGGA+TGG	+	chr2_4:6623779-6623798	Msa0311730:three_prime_UTR	35.0%
	GAGTTAAGATGAATCAAGCT+TGG	+	chr2_4:6624015-6624034	Msa0311730:three_prime_UTR	35.0%
!!	ATCAAGCTTGGCTCTATTAT+AGG	+	chr2_4:6624027-6624046	Msa0311730:three_prime_UTR	35.0%
	CTATTGAAAGCAAGCACAAG+TGG	-	chr2_4:6620555-6620574	None:intergenic	40.0%
!	ACGTTGTTGTTGTTGTTGGT+GGG	-	chr2_4:6620589-6620608	None:intergenic	40.0%
!	TACGTTGTTGTTGTTGTTGG+TGG	-	chr2_4:6620590-6620609	None:intergenic	40.0%
	TTTGGAGAAGCAACAAGAGA+AGG	+	chr2_4:6620766-6620785	Msa0311730:CDS	40.0%
!	TGAAGAGAATGAGAGGCTTT+TGG	+	chr2_4:6620823-6620842	Msa0311730:CDS	40.0%
!!	TTTGGAGGGTATAGCTGTTT+TGG	+	chr2_4:6620841-6620860	Msa0311730:CDS	40.0%
	CAAACTGCAAATGGAACTTG+GGG	+	chr2_4:6620896-6620915	Msa0311730:CDS	40.0%
	AAACTGCAAATGGAACTTGG+GGG	+	chr2_4:6620897-6620916	Msa0311730:CDS	40.0%
	CCCATCCAAAGTCTAAACAT+AGG	-	chr2_4:6621401-6621420	None:intergenic	40.0%
!!	GTCCCAAGTTTGATGATGAT+AGG	-	chr2_4:6621504-6621523	None:intergenic	40.0%
	ATCACGTTCCTCATAGATTC+GGG	-	chr2_4:6621750-6621769	None:intergenic	40.0%
	TCTATGAGGAACGTGATTTG+TGG	+	chr2_4:6621753-6621772	Msa0311730:intron	40.0%
!!	CGTTTATTTTCTCATCGTGG+TGG	+	chr2_4:6621847-6621866	Msa0311730:intron	40.0%
	TGGCCAACGAAAGTCTAAAA+TGG	+	chr2_4:6622019-6622038	Msa0311730:intron	40.0%
!!	CTGGTTTGCTGTTACTTACA+AGG	+	chr2_4:6622081-6622100	Msa0311730:intron	40.0%
	GCTTCATGTTCTTCCCAAAT+CGG	-	chr2_4:6622168-6622187	None:intergenic	40.0%
!	GAAGTTGAAAAGAGCACAGA+TGG	-	chr2_4:6622421-6622440	None:intergenic	40.0%
	TAATGATGAAGGTGACTGTC+AGG	-	chr2_4:6622482-6622501	None:intergenic	40.0%
	ACAGTCACCTTCATCATTAC+TGG	+	chr2_4:6622483-6622502	Msa0311730:CDS	40.0%
!	TCACAGACAAGTTTTCACAG+AGG	+	chr2_4:6622604-6622623	Msa0311730:intron	40.0%
!!!	CCCGCTTTAGAGGATTTTAA+AGG	+	chr2_4:6622785-6622804	Msa0311730:intron	40.0%
	ATTGGTCCAAAGTGCAACTT+TGG	-	chr2_4:6622817-6622836	None:intergenic	40.0%
	AAAACCATCCTCCATCACAT+TGG	-	chr2_4:6622835-6622854	None:intergenic	40.0%
	TACAGCAAGGACAGACAAAA+TGG	-	chr2_4:6623049-6623068	None:intergenic	40.0%
!	TCCTTGCTGTACATTATGTC+AGG	+	chr2_4:6623058-6623077	Msa0311730:CDS	40.0%
	ACCTGACATAATGTACAGCA+AGG	-	chr2_4:6623062-6623081	None:intergenic	40.0%
	GTACCTTTGTATCATGATGC+AGG	+	chr2_4:6623230-6623249	Msa0311730:intron	40.0%
	ACTCCTGCATCATGATACAA+AGG	-	chr2_4:6623236-6623255	None:intergenic	40.0%
	TATCGAGTCGATCAAGCTTA+CGG	+	chr2_4:6623374-6623393	Msa0311730:CDS	40.0%
	GACCAAAGTGCATGTAAGTT+TGG	-	chr2_4:6623727-6623746	None:intergenic	40.0%
	AAACTATATACTACAGGGGC+TGG	-	chr2_4:6623900-6623919	None:intergenic	40.0%
	GTAGGCCTACTTGAGAATAA+AGG	-	chr2_4:6623991-6624010	None:intergenic	40.0%
!	CGTTGTTGTTGTTGTTGGTG+GGG	-	chr2_4:6620588-6620607	None:intergenic	45.0%
	GAAGCAACAAGAGAAGGTGA+TGG	+	chr2_4:6620772-6620791	Msa0311730:CDS	45.0%
!	ACAAGAGAAGGTGATGGTGA+TGG	+	chr2_4:6620778-6620797	Msa0311730:CDS	45.0%
!!	AAGGTGATGGTGATGGATGA+AGG	+	chr2_4:6620785-6620804	Msa0311730:CDS	45.0%
!!	ATGGTGATGGATGAAGGAGA+AGG	+	chr2_4:6620791-6620810	Msa0311730:CDS	45.0%
	GATGAAGGAGAAGGAAGAGA+AGG	+	chr2_4:6620800-6620819	Msa0311730:CDS	45.0%
	GAGAAGGTGAAGAGAATGAG+AGG	+	chr2_4:6620816-6620835	Msa0311730:CDS	45.0%
!	AGAGAATGAGAGGCTTTTGG+AGG	+	chr2_4:6620826-6620845	Msa0311730:CDS	45.0%
!	GAGAATGAGAGGCTTTTGGA+GGG	+	chr2_4:6620827-6620846	Msa0311730:CDS	45.0%
!!!	AGGAGTTTGGTGGTGTTTTG+AGG	+	chr2_4:6620957-6620976	Msa0311730:CDS	45.0%
!!!	TTGGTGGTGTTTTGAGGATG+TGG	+	chr2_4:6620963-6620982	Msa0311730:CDS	45.0%
!!!	TGGTGGTGTTTTGAGGATGT+GGG	+	chr2_4:6620964-6620983	Msa0311730:CDS	45.0%
	ACAGAAAGTCAGGACAGAAC+CGG	-	chr2_4:6621782-6621801	None:intergenic	45.0%
!!	AAACGCGCTGTTTCCCTTTT+GGG	-	chr2_4:6621832-6621851	None:intergenic	45.0%
!!	TAAACGCGCTGTTTCCCTTT+TGG	-	chr2_4:6621833-6621852	None:intergenic	45.0%
!!	GCGCGTTTATTTTCTCATCG+TGG	+	chr2_4:6621844-6621863	Msa0311730:intron	45.0%
!!	TATTTTCTCATCGTGGTGGG+AGG	+	chr2_4:6621851-6621870	Msa0311730:intron	45.0%
!!	ATTTTCTCATCGTGGTGGGA+GGG	+	chr2_4:6621852-6621871	Msa0311730:intron	45.0%
!!!	TTTTGAGTTACCGAGATGCC+TGG	+	chr2_4:6621896-6621915	Msa0311730:intron	45.0%
	CAAAACCATACCAGGCATCT+CGG	-	chr2_4:6621909-6621928	None:intergenic	45.0%
	TTCTTCCCAAATCGGTAGCA+GGG	-	chr2_4:6622160-6622179	None:intergenic	45.0%
	GTGATGTCTCGTGACGATAA+AGG	-	chr2_4:6622448-6622467	None:intergenic	45.0%
	GTCACCTTCATCATTACTGG+TGG	+	chr2_4:6622486-6622505	Msa0311730:CDS	45.0%
	TGCTCCACCAGTAATGATGA+AGG	-	chr2_4:6622493-6622512	None:intergenic	45.0%
!	TTTCACAGAGGTATGCTGTC+TGG	+	chr2_4:6622616-6622635	Msa0311730:intron	45.0%
	TCCTAACTTTCCCGCTTTAG+AGG	+	chr2_4:6622775-6622794	Msa0311730:intron	45.0%
!!	TCCTCTAAAGCGGGAAAGTT+AGG	-	chr2_4:6622779-6622798	None:intergenic	45.0%
	TGCACTTTGGACCAATGTGA+TGG	+	chr2_4:6622821-6622840	Msa0311730:intron	45.0%
	ACTTTGGACCAATGTGATGG+AGG	+	chr2_4:6622824-6622843	Msa0311730:intron	45.0%
	TTGCTTGCATGCATATGTGC+TGG	+	chr2_4:6622871-6622890	Msa0311730:intron	45.0%
	CATGCATATGTGCTGGAGTA+TGG	+	chr2_4:6622878-6622897	Msa0311730:intron	45.0%
	CATATGTGCTGGAGTATGGT+TGG	+	chr2_4:6622882-6622901	Msa0311730:intron	45.0%
!!	TTTTCAGGGTAGCGACAGTA+TGG	+	chr2_4:6623007-6623026	Msa0311730:intron	45.0%
	ACAACGTTCAAGATGGCACT+TGG	+	chr2_4:6623274-6623293	Msa0311730:CDS	45.0%
	CCTAATGCCACCTATGCATA+TGG	-	chr2_4:6623316-6623335	None:intergenic	45.0%
	CCATATGCATAGGTGGCATT+AGG	+	chr2_4:6623313-6623332	Msa0311730:CDS	45.0%
	TGCATAGGTGGCATTAGGAA+TGG	+	chr2_4:6623318-6623337	Msa0311730:CDS	45.0%
	GCATAGGTGGCATTAGGAAT+GGG	+	chr2_4:6623319-6623338	Msa0311730:CDS	45.0%
!!	GTAAGCTTGATCGACTCGAT+AGG	-	chr2_4:6623375-6623394	None:intergenic	45.0%
!	CTAAGAGGTAGTGGCAATCA+CGG	+	chr2_4:6623579-6623598	Msa0311730:three_prime_UTR	45.0%
!	TAAGAGGTAGTGGCAATCAC+GGG	+	chr2_4:6623580-6623599	Msa0311730:three_prime_UTR	45.0%
!	TGTTGTTGTTGGTGGGGTGT+AGG	-	chr2_4:6620582-6620601	None:intergenic	50.0%
	AATGGAACTTGGGGGAAGCT+TGG	+	chr2_4:6620905-6620924	Msa0311730:CDS	50.0%
	AGATGATGAACAGCGTGGTG+AGG	+	chr2_4:6620937-6620956	Msa0311730:CDS	50.0%
!!!	GGTGTTTTGAGGATGTGGGA+AGG	+	chr2_4:6620968-6620987	Msa0311730:CDS	50.0%
	TAGACTTTGGATGGGGGCTA+TGG	+	chr2_4:6621406-6621425	Msa0311730:intron	50.0%
	GCATGTGGCGTATAACAGGA+TGG	-	chr2_4:6621728-6621747	None:intergenic	50.0%
	TCCTCATAGATTCGGGCATG+TGG	-	chr2_4:6621743-6621762	None:intergenic	50.0%
!!	AACGCGCTGTTTCCCTTTTG+GGG	-	chr2_4:6621831-6621850	None:intergenic	50.0%
!!	TTTTCTCATCGTGGTGGGAG+GGG	+	chr2_4:6621853-6621872	Msa0311730:intron	50.0%
	AGTTACCGAGATGCCTGGTA+TGG	+	chr2_4:6621901-6621920	Msa0311730:intron	50.0%
!	AGTTGTCCCTGCTACCGATT+TGG	+	chr2_4:6622151-6622170	Msa0311730:intron	50.0%
!!	GTTGTCCCTGCTACCGATTT+GGG	+	chr2_4:6622152-6622171	Msa0311730:intron	50.0%
	GTTCTTCCCAAATCGGTAGC+AGG	-	chr2_4:6622161-6622180	None:intergenic	50.0%
!	AACATGAAGCGAGCTGGTCT+TGG	+	chr2_4:6622178-6622197	Msa0311730:CDS	50.0%
!	AGAGGTATGCTGTCTGGACA+TGG	+	chr2_4:6622622-6622641	Msa0311730:intron	50.0%
!	TGGACCAATGTGATGGAGGA+TGG	+	chr2_4:6622828-6622847	Msa0311730:intron	50.0%
	GTTCAAGATGGCACTTGGCA+CGG	+	chr2_4:6623279-6623298	Msa0311730:CDS	50.0%
	GGTGACACCATATGCATAGG+TGG	+	chr2_4:6623306-6623325	Msa0311730:CDS	50.0%
!!	TTGATCGACTCGATAGGAGG+AGG	-	chr2_4:6623369-6623388	None:intergenic	50.0%
!!	AGCTTGATCGACTCGATAGG+AGG	-	chr2_4:6623372-6623391	None:intergenic	50.0%
	TTGCCACTACCTCTTAGTCC+CGG	-	chr2_4:6623576-6623595	None:intergenic	50.0%
	GGTGGAGATGATGAACAGCG+TGG	+	chr2_4:6620932-6620951	Msa0311730:CDS	55.0%
	GAACAGCGTGGTGAGGAGTT+TGG	+	chr2_4:6620944-6620963	Msa0311730:CDS	55.0%
	ACTTTGGATGGGGGCTATGG+AGG	+	chr2_4:6621409-6621428	Msa0311730:intron	55.0%
	TCGGGCATGTGGCGTATAAC+AGG	-	chr2_4:6621732-6621751	None:intergenic	55.0%
	GCCACATGCCCGAATCTATG+AGG	+	chr2_4:6621739-6621758	Msa0311730:intron	55.0%
!	GGAACGTGATTTGTGGCGAC+CGG	+	chr2_4:6621760-6621779	Msa0311730:intron	55.0%
	GGGAAGAACATGAAGCGAGC+TGG	+	chr2_4:6622172-6622191	Msa0311730:CDS	55.0%
	TCAGGGTAGCGACAGTATGG+TGG	+	chr2_4:6623010-6623029	Msa0311730:intron	55.0%
	AAGATGGCACTTGGCACGGT+CGG	+	chr2_4:6623283-6623302	Msa0311730:CDS	55.0%
	CGGGGTGACACCATATGCAT+AGG	+	chr2_4:6623303-6623322	Msa0311730:CDS	55.0%
	TTGTATCCCTGGTGTCTGCC+CGG	+	chr2_4:6623497-6623516	Msa0311730:three_prime_UTR	55.0%
	AATATGCCGGGCAGACACCA+GGG	-	chr2_4:6623506-6623525	None:intergenic	55.0%
	AAATATGCCGGGCAGACACC+AGG	-	chr2_4:6623507-6623526	None:intergenic	55.0%
	CCTCTTAGTCCCGGTACGAT+CGG	-	chr2_4:6623567-6623586	None:intergenic	55.0%
	GTACCGGGACTAAGAGGTAG+TGG	+	chr2_4:6623570-6623589	Msa0311730:three_prime_UTR	55.0%
	GGAACTTGGGGGAAGCTTGG+AGG	+	chr2_4:6620908-6620927	Msa0311730:CDS	60.0%
	ACTTGGGGGAAGCTTGGAGG+AGG	+	chr2_4:6620911-6620930	Msa0311730:CDS	60.0%
	CAGCGTGGTGAGGAGTTTGG+TGG	+	chr2_4:6620947-6620966	Msa0311730:CDS	60.0%
	AGATGGCACTTGGCACGGTC+GGG	+	chr2_4:6623284-6623303	Msa0311730:CDS	60.0%
!!	GCCAACAGCACCGATCGTAC+CGG	+	chr2_4:6623554-6623573	Msa0311730:three_prime_UTR	60.0%
!!	CCAACAGCACCGATCGTACC+GGG	+	chr2_4:6623555-6623574	Msa0311730:three_prime_UTR	60.0%
	CCGATCGTACCGGGACTAAG+AGG	+	chr2_4:6623564-6623583	Msa0311730:three_prime_UTR	60.0%
	TGGGGGAAGCTTGGAGGAGG+TGG	+	chr2_4:6620914-6620933	Msa0311730:CDS	65.0%
	GATGGCACTTGGCACGGTCG+GGG	+	chr2_4:6623285-6623304	Msa0311730:CDS	65.0%
!!	CCCGGTACGATCGGTGCTGT+TGG	-	chr2_4:6623558-6623577	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	6620542	6624126	6620542	ID=Msa0311730;Name=Msa0311730
chr2_4	mRNA	6620542	6624126	6620542	ID=Msa0311730-mRNA-1;Parent=Msa0311730;Name=Msa0311730-mRNA-1;_AED=0.15;_eAED=0.15;_QI=216\|1\|1\|1\|1\|1\|5\|710\|247
chr2_4	exon	6620542	6621041	6620542	ID=Msa0311730-mRNA-1:exon:2549;Parent=Msa0311730-mRNA-1
chr2_4	exon	6622153	6622216	6622153	ID=Msa0311730-mRNA-1:exon:2550;Parent=Msa0311730-mRNA-1
chr2_4	exon	6622396	6622560	6622396	ID=Msa0311730-mRNA-1:exon:2551;Parent=Msa0311730-mRNA-1
chr2_4	exon	6623014	6623079	6623014	ID=Msa0311730-mRNA-1:exon:2552;Parent=Msa0311730-mRNA-1
chr2_4	exon	6623252	6624126	6623252	ID=Msa0311730-mRNA-1:exon:2553;Parent=Msa0311730-mRNA-1
chr2_4	five_prime_UTR	6620542	6620757	6620542	ID=Msa0311730-mRNA-1:five_prime_utr;Parent=Msa0311730-mRNA-1
chr2_4	CDS	6620758	6621041	6620758	ID=Msa0311730-mRNA-1:cds;Parent=Msa0311730-mRNA-1
chr2_4	CDS	6622153	6622216	6622153	ID=Msa0311730-mRNA-1:cds;Parent=Msa0311730-mRNA-1
chr2_4	CDS	6622396	6622560	6622396	ID=Msa0311730-mRNA-1:cds;Parent=Msa0311730-mRNA-1
chr2_4	CDS	6623014	6623079	6623014	ID=Msa0311730-mRNA-1:cds;Parent=Msa0311730-mRNA-1
chr2_4	CDS	6623252	6623416	6623252	ID=Msa0311730-mRNA-1:cds;Parent=Msa0311730-mRNA-1
chr2_4	three_prime_UTR	6623417	6624126	6623417	ID=Msa0311730-mRNA-1:three_prime_utr;Parent=Msa0311730-mRNA-1

Gene Sequence

>Msa0311730

ATGGGTGATTTGGAGAAGCAACAAGAGAAGGTGATGGTGATGGATGAAGGAGAAGGAAGAGAAGGTGAAGAGAATGAGAGGCTTTTGGAGGGTATAGCTGTTTTGGATTTTGATATGCTTTGTTCGTCTGTTGCTTTACAAACTGCAAATGGAACTTGGGGGAAGCTTGGAGGAGGTGGAGATGATGAACAGCGTGGTGAGGAGTTTGGTGGTGTTTTGAGGATGTGGGAAGGTGAAGTTCTTGATTGTTTCGAACATCGTCGAATTGCTCTTGAATCTTCATGTTGTCCCTGCTACCGATTTGGGAAGAACATGAAGCGAGCTGGTCTTGGTTCTTGCTATATTCAGGCATTTGTATATTTTCTTCTAGCCATCTGTGCTCTTTTCAACTTCATAGCCTTTATCGTCACGAGACATCACTACTTTCTATACCTGACAGTCACCTTCATCATTACTGGTGGAGCATATTTAGGATTCTACCGAACTCGTATGCGAAAGAAATTCAACATCAAGGGTAGCGACAGTATGGTGGACGATTGTGTTTACCATTTTGTCTGTCCTTGCTGTACATTATGTCAGGAGTCAAGAACACTAGAGATTAACAACGTTCAAGATGGCACTTGGCACGGTCGGGGTGACACCATATGCATAGGTGGCATTAGGAATGGGAGTAAAGCACTCTCTGAGATGATTCCTCCTCCTATCGAGTCGATCAAGCTTACGGATGAAAATTACACCTTATAG

Protein sequence

>Msa0311730

MGDLEKQQEKVMVMDEGEGREGEENERLLEGIAVLDFDMLCSSVALQTANGTWGKLGGGGDDEQRGEEFGGVLRMWEGEVLDCFEHRRIALESSCCPCYRFGKNMKRAGLGSCYIQAFVYFLLAICALFNFIAFIVTRHHYFLYLTVTFIITGGAYLGFYRTRMRKKFNIKGSDSMVDDCVYHFVCPCCTLCQESRTLEINNVQDGTWHGRGDTICIGGIRNGSKALSEMIPPPIESIKLTDENYTL*