AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0970990


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 9 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAGGTGGAAGAGCATTGT+TGG 0.392371 6_4:-2655536 Msa0970990:CDS
AAGAGCATTGTTGGTTTCAG+TGG 0.499187 6_4:-2655527 Msa0970990:CDS
AAGAACATTGTTGATTTCAG+TGG 0.510674 6_4:-2654540 Msa0970990:CDS
TCTCGTCAAAACCGTGGAAG+AGG 0.517503 6_4:-2655832 Msa0970990:intron
GAGAGAGAGTGAATCAGAAG+AGG 0.523266 6_4:+2655889 None:intergenic
AGCATTGTTGGTTTCAGTGG+AGG 0.538384 6_4:-2655524 Msa0970990:CDS
AAATCAAATATTGAAATCAA+CGG 0.594348 6_4:-2656000 None:intergenic
TTCCTCTCTCGTCAAAACCG+TGG 0.622333 6_4:-2655838 Msa0970990:CDS
AAGAATCCGAACCTCTTCCA+CGG 0.634242 6_4:+2655821 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAATTTACCTTTATATT+GGG + chr6_4:2655508-2655527 None:intergenic 15.0%
!! AAATCAATTTACCTTTATAT+TGG + chr6_4:2655509-2655528 None:intergenic 15.0%
!! AATAAGCTGAAAAAAATTTA+TGG - chr6_4:2655648-2655667 Msa0970990:intron 15.0%
!! AATCAATTTACCTTTATATT+GGG + chr6_4:2655508-2655527 None:intergenic 15.0%
!! AAATCAATTTACCTTTATAT+TGG + chr6_4:2655509-2655528 None:intergenic 15.0%
!! AATAAGCTGAAAAAAATTTA+TGG - chr6_4:2655648-2655667 Msa0970990:intron 15.0%
!!! ATTTTGTTGAATGTTAGTTT+GGG - chr6_4:2655010-2655029 Msa0970990:intron 20.0%
!!! ATTTTGTTGAATGTTAGTTT+GGG - chr6_4:2655010-2655029 Msa0970990:intron 20.0%
!! GATTTTGTTGAATGTTAGTT+TGG - chr6_4:2655009-2655028 Msa0970990:intron 25.0%
!! AAATTTTGTGTTTGAATGCT+AGG - chr6_4:2655045-2655064 Msa0970990:intron 25.0%
!! AATTTTGTGTTTGAATGCTA+GGG - chr6_4:2655046-2655065 Msa0970990:intron 25.0%
! ATAAGCTGAAAACAACTTAT+TGG - chr6_4:2655144-2655163 Msa0970990:intron 25.0%
!! AATTTTCTGTGTTCAGTTTT+AGG - chr6_4:2655219-2655238 Msa0970990:intron 25.0%
!!! TAAGCTATTTCTATTGTGAA+AGG - chr6_4:2655319-2655338 Msa0970990:intron 25.0%
!! AGTTATATGTTGTTTGCATA+AGG - chr6_4:2655535-2655554 Msa0970990:CDS 25.0%
! ATGGTTATGATTTCTATCTT+TGG - chr6_4:2655785-2655804 Msa0970990:intron 25.0%
!! GATTTTGTTGAATGTTAGTT+TGG - chr6_4:2655009-2655028 Msa0970990:intron 25.0%
!! AAATTTTGTGTTTGAATGCT+AGG - chr6_4:2655045-2655064 Msa0970990:intron 25.0%
!! AATTTTGTGTTTGAATGCTA+GGG - chr6_4:2655046-2655065 Msa0970990:intron 25.0%
! ATAAGCTGAAAACAACTTAT+TGG - chr6_4:2655144-2655163 Msa0970990:intron 25.0%
!! AATTTTCTGTGTTCAGTTTT+AGG - chr6_4:2655219-2655238 Msa0970990:intron 25.0%
!!! TAAGCTATTTCTATTGTGAA+AGG - chr6_4:2655319-2655338 Msa0970990:intron 25.0%
!! AGTTATATGTTGTTTGCATA+AGG - chr6_4:2655535-2655554 Msa0970990:CDS 25.0%
! ATGGTTATGATTTCTATCTT+TGG - chr6_4:2655785-2655804 Msa0970990:intron 25.0%
!!! TTTTTTTGTGTTTGATGCAG+AGG - chr6_4:2654936-2654955 Msa0970990:intron 30.0%
TTGAGTTTATCCATTAGCAT+AGG + chr6_4:2655723-2655742 None:intergenic 30.0%
!! TTTCTATCTTTGGTGTTTCA+AGG - chr6_4:2655795-2655814 Msa0970990:intron 30.0%
!!! TTTTTTTGTGTTTGATGCAG+AGG - chr6_4:2654936-2654955 Msa0970990:intron 30.0%
!! AAGAACATTGTTGATTTCAG+TGG - chr6_4:2655949-2655968 Msa0970990:CDS 30.0%
!!! TTTTTTTGTGTTTGATGCAG+AGG - chr6_4:2654936-2654955 Msa0970990:intron 30.0%
TTGAGTTTATCCATTAGCAT+AGG + chr6_4:2655723-2655742 None:intergenic 30.0%
!! TTTCTATCTTTGGTGTTTCA+AGG - chr6_4:2655795-2655814 Msa0970990:intron 30.0%
!!! TTTTTTTGTGTTTGATGCAG+AGG - chr6_4:2654936-2654955 Msa0970990:intron 30.0%
!! AAGAACATTGTTGATTTCAG+TGG - chr6_4:2655949-2655968 Msa0970990:CDS 30.0%
!!! TTTTTTCGTGTTTGATGCAG+TGG - chr6_4:2654782-2654801 Msa0970990:intron 35.0%
!!! AAGAGCATTTTCGATTTCAG+TGG - chr6_4:2654808-2654827 Msa0970990:intron 35.0%
!!! GTTCAGTTTTAGGTGATGTA+TGG - chr6_4:2655229-2655248 Msa0970990:intron 35.0%
TATATTGGGACTGTTTGGAT+TGG + chr6_4:2655494-2655513 None:intergenic 35.0%
TCCAAACAGTCCCAATATAA+AGG - chr6_4:2655495-2655514 Msa0970990:intron 35.0%
ACCTTTATATTGGGACTGTT+TGG + chr6_4:2655499-2655518 None:intergenic 35.0%
!! GCTGTTTTCATTAGCTATCT+TGG - chr6_4:2655613-2655632 Msa0970990:intron 35.0%
ACAATCCAAACAAACCCTTA+CGG - chr6_4:2655749-2655768 Msa0970990:intron 35.0%
TATATCCAAACATGCCGTAA+GGG + chr6_4:2655766-2655785 None:intergenic 35.0%
ATATATCCAAACATGCCGTA+AGG + chr6_4:2655767-2655786 None:intergenic 35.0%
TTACGGCATGTTTGGATATA+TGG - chr6_4:2655766-2655785 Msa0970990:intron 35.0%
AAAAGAAATCCTTGTGTGTG+TGG - chr6_4:2655827-2655846 Msa0970990:intron 35.0%
!!! TTTTTTCGTGTTTGATGCAG+TGG - chr6_4:2654782-2654801 Msa0970990:intron 35.0%
!!! AAGAGCATTTTCGATTTCAG+TGG - chr6_4:2654808-2654827 Msa0970990:intron 35.0%
!!! GTTCAGTTTTAGGTGATGTA+TGG - chr6_4:2655229-2655248 Msa0970990:intron 35.0%
TATATTGGGACTGTTTGGAT+TGG + chr6_4:2655494-2655513 None:intergenic 35.0%
TCCAAACAGTCCCAATATAA+AGG - chr6_4:2655495-2655514 Msa0970990:intron 35.0%
ACCTTTATATTGGGACTGTT+TGG + chr6_4:2655499-2655518 None:intergenic 35.0%
!! GCTGTTTTCATTAGCTATCT+TGG - chr6_4:2655613-2655632 Msa0970990:intron 35.0%
ACAATCCAAACAAACCCTTA+CGG - chr6_4:2655749-2655768 Msa0970990:intron 35.0%
TATATCCAAACATGCCGTAA+GGG + chr6_4:2655766-2655785 None:intergenic 35.0%
ATATATCCAAACATGCCGTA+AGG + chr6_4:2655767-2655786 None:intergenic 35.0%
TTACGGCATGTTTGGATATA+TGG - chr6_4:2655766-2655785 Msa0970990:intron 35.0%
AAAAGAAATCCTTGTGTGTG+TGG - chr6_4:2655827-2655846 Msa0970990:intron 35.0%
TTCCACGAATTTCTACCTTC+GGG + chr6_4:2654841-2654860 None:intergenic 40.0%
CTTCCACGAATTTCTACCTT+CGG + chr6_4:2654842-2654861 None:intergenic 40.0%
! TTTTGTGTTTGATGCAGAGG+TGG - chr6_4:2654939-2654958 Msa0970990:intron 40.0%
!! AAGAGCATTGTTGGTTTCAG+TGG - chr6_4:2654962-2654981 Msa0970990:intron 40.0%
! GGGTTTAGTGTTTTGTGTTG+TGG - chr6_4:2655066-2655085 Msa0970990:intron 40.0%
TCCATTAGCATAGGTTGTGT+GGG + chr6_4:2655714-2655733 None:intergenic 40.0%
ATCCATTAGCATAGGTTGTG+TGG + chr6_4:2655715-2655734 None:intergenic 40.0%
! TTTTGTGTTTGATGCAGAGG+TGG - chr6_4:2654939-2654958 Msa0970990:intron 40.0%
!! TTCAGTGGAGATTTTGACGA+AGG - chr6_4:2655964-2655983 Msa0970990:CDS 40.0%
TTCCACGAATTTCTACCTTC+GGG + chr6_4:2654841-2654860 None:intergenic 40.0%
CTTCCACGAATTTCTACCTT+CGG + chr6_4:2654842-2654861 None:intergenic 40.0%
! TTTTGTGTTTGATGCAGAGG+TGG - chr6_4:2654939-2654958 Msa0970990:intron 40.0%
!! AAGAGCATTGTTGGTTTCAG+TGG - chr6_4:2654962-2654981 Msa0970990:intron 40.0%
! GGGTTTAGTGTTTTGTGTTG+TGG - chr6_4:2655066-2655085 Msa0970990:intron 40.0%
TCCATTAGCATAGGTTGTGT+GGG + chr6_4:2655714-2655733 None:intergenic 40.0%
ATCCATTAGCATAGGTTGTG+TGG + chr6_4:2655715-2655734 None:intergenic 40.0%
! TTTTGTGTTTGATGCAGAGG+TGG - chr6_4:2654939-2654958 Msa0970990:intron 40.0%
!! TTCAGTGGAGATTTTGACGA+AGG - chr6_4:2655964-2655983 Msa0970990:CDS 40.0%
GAGAGAGAGTGAATCAGAAG+AGG + chr6_4:2654603-2654622 None:intergenic 45.0%
TCAAAACCGTGGAAGAGGTT+CGG - chr6_4:2654662-2654681 Msa0970990:intron 45.0%
AAGAATCCGAACCTCTTCCA+CGG + chr6_4:2654671-2654690 None:intergenic 45.0%
! TTCAGTGGAGATTTTCCCGA+AGG - chr6_4:2654823-2654842 Msa0970990:intron 45.0%
TTCCCGAAGGTAGAAATTCG+TGG - chr6_4:2654836-2654855 Msa0970990:intron 45.0%
!! AGCATTGTTGGTTTCAGTGG+AGG - chr6_4:2654965-2654984 Msa0970990:intron 45.0%
!! TTCAGTGGAGGTTTTGACGA+AGG - chr6_4:2654977-2654996 Msa0970990:intron 45.0%
TAGGTTGTGTGGGACTGTTT+AGG + chr6_4:2655704-2655723 None:intergenic 45.0%
TCCCACACAACCTATGCTAA+TGG - chr6_4:2655710-2655729 Msa0970990:intron 45.0%
! ACATGCCGTAAGGGTTTGTT+TGG + chr6_4:2655757-2655776 None:intergenic 45.0%
ACAAACCCTTACGGCATGTT+TGG - chr6_4:2655758-2655777 Msa0970990:intron 45.0%
AAATCCTTGTGTGTGTGGCA+TGG - chr6_4:2655832-2655851 Msa0970990:intron 45.0%
AATCCTTGTGTGTGTGGCAT+GGG - chr6_4:2655833-2655852 Msa0970990:CDS 45.0%
GAGAGAGAGTGAATCAGAAG+AGG + chr6_4:2654603-2654622 None:intergenic 45.0%
TCAAAACCGTGGAAGAGGTT+CGG - chr6_4:2654662-2654681 Msa0970990:intron 45.0%
AAGAATCCGAACCTCTTCCA+CGG + chr6_4:2654671-2654690 None:intergenic 45.0%
! TTCAGTGGAGATTTTCCCGA+AGG - chr6_4:2654823-2654842 Msa0970990:intron 45.0%
TTCCCGAAGGTAGAAATTCG+TGG - chr6_4:2654836-2654855 Msa0970990:intron 45.0%
!! AGCATTGTTGGTTTCAGTGG+AGG - chr6_4:2654965-2654984 Msa0970990:intron 45.0%
!! TTCAGTGGAGGTTTTGACGA+AGG - chr6_4:2654977-2654996 Msa0970990:intron 45.0%
TAGGTTGTGTGGGACTGTTT+AGG + chr6_4:2655704-2655723 None:intergenic 45.0%
TCCCACACAACCTATGCTAA+TGG - chr6_4:2655710-2655729 Msa0970990:intron 45.0%
! ACATGCCGTAAGGGTTTGTT+TGG + chr6_4:2655757-2655776 None:intergenic 45.0%
ACAAACCCTTACGGCATGTT+TGG - chr6_4:2655758-2655777 Msa0970990:intron 45.0%
AAATCCTTGTGTGTGTGGCA+TGG - chr6_4:2655832-2655851 Msa0970990:intron 45.0%
AATCCTTGTGTGTGTGGCAT+GGG - chr6_4:2655833-2655852 Msa0970990:CDS 45.0%
TTCCTCTCTCGTCAAAACCG+TGG - chr6_4:2654651-2654670 Msa0970990:intron 50.0%
!! TTCCACGGTTTTGACGAGAG+AGG + chr6_4:2654656-2654675 None:intergenic 50.0%
TCTCGTCAAAACCGTGGAAG+AGG - chr6_4:2654657-2654676 Msa0970990:intron 50.0%
!! CAGAGGTGGAAGAGCATTGT+TGG - chr6_4:2654953-2654972 Msa0970990:intron 50.0%
ATCCTTGTGTGTGTGGCATG+GGG - chr6_4:2655834-2655853 Msa0970990:CDS 50.0%
TTCCTCTCTCGTCAAAACCG+TGG - chr6_4:2654651-2654670 Msa0970990:intron 50.0%
!! TTCCACGGTTTTGACGAGAG+AGG + chr6_4:2654656-2654675 None:intergenic 50.0%
TCTCGTCAAAACCGTGGAAG+AGG - chr6_4:2654657-2654676 Msa0970990:intron 50.0%
!! CAGAGGTGGAAGAGCATTGT+TGG - chr6_4:2654953-2654972 Msa0970990:intron 50.0%
ATCCTTGTGTGTGTGGCATG+GGG - chr6_4:2655834-2655853 Msa0970990:CDS 50.0%
AGCCCCATGCCACACACACA+AGG + chr6_4:2655839-2655858 None:intergenic 60.0%
AGCCCCATGCCACACACACA+AGG + chr6_4:2655839-2655858 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 2654503 2656008 2654503 ID=Msa0970990;Name=Msa0970990
chr6_4 mRNA 2654503 2656008 2654503 ID=Msa0970990-mRNA-1;Parent=Msa0970990;Name=Msa0970990-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0.5|0.66|1|1|1|3|12|90
chr6_4 exon 2654503 2654568 2654503 ID=Msa0970990-mRNA-1:exon:1339;Parent=Msa0970990-mRNA-1
chr6_4 exon 2655513 2655555 2655513 ID=Msa0970990-mRNA-1:exon:1338;Parent=Msa0970990-mRNA-1
chr6_4 exon 2655833 2656008 2655833 ID=Msa0970990-mRNA-1:exon:1337;Parent=Msa0970990-mRNA-1
chr6_4 CDS 2655833 2656008 2655833 ID=Msa0970990-mRNA-1:cds;Parent=Msa0970990-mRNA-1
chr6_4 CDS 2655513 2655555 2655513 ID=Msa0970990-mRNA-1:cds;Parent=Msa0970990-mRNA-1
chr6_4 CDS 2654515 2654568 2654515 ID=Msa0970990-mRNA-1:cds;Parent=Msa0970990-mRNA-1
chr6_4 three_prime_UTR 2654503 2654514 2654503 ID=Msa0970990-mRNA-1:three_prime_utr;Parent=Msa0970990-mRNA-1
Gene Sequence

>Msa0970990

AATCAACGGTTCAGATCTCTCTATCTCAGCGAGTGTGTGAACGTTGCTGAGTTCACTCAGCGCGTGAACAAAACTCGTTCTTCTTCGTCGTCTTCTTCCTCTTCTGATTCACTCTCTCTCATTCTTCGTTTTCTTCTTCATTTTCATCTTCCTCTCTCGTCAAAACCGTGGAAGAGAGGTGGAAGAGCATTGTTGGTTTCAGTGGAGGTTTTGACGAAGAGGTGGAAGAACATTGTTGATTTCAGTGGAGATTTTGACGAAGGTAGAATTTAG

Protein sequence

>Msa0970990

NQRFRSLYLSECVNVAEFTQRVNKTRSSSSSSSSSDSLSLILRFLLHFHLPLSSKPWKRGGRALLVSVEVLTKRWKNIVDFSGDFDEGRI*