AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0543850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0543850 MtrunA17_Chr4g0024351 97.450 549 12 1 1 547 186 734 0.0 1088
Msa0543850 MtrunA17_Chr2g0322731 85.844 551 68 5 1 547 226 770 0.0 928
Msa0543850 MtrunA17_Chr7g0246631 54.720 572 223 11 1 546 217 778 0.0 597
Msa0543850 MtrunA17_Chr8g0350321 59.600 500 189 6 57 546 314 810 0.0 593
Msa0543850 MtrunA17_Chr5g0394111 55.796 509 203 8 46 542 148 646 0.0 553
Msa0543850 MtrunA17_Chr1g0203671 53.204 515 222 6 37 542 285 789 0.0 548
Msa0543850 MtrunA17_Chr6g0481041 45.172 580 279 11 1 547 155 728 1.99e-165 487
Msa0543850 MtrunA17_Chr2g0331581 44.869 497 259 6 57 545 303 792 5.34e-154 460
Msa0543850 MtrunA17_Chr5g0410711 44.970 507 259 9 55 546 281 782 4.96e-147 442
Msa0543850 MtrunA17_Chr6g0472471 42.545 550 287 10 10 542 228 765 1.47e-138 419
Msa0543850 MtrunA17_Chr2g0292731 36.566 495 303 7 55 542 58 548 8.94e-106 328
Msa0543850 MtrunA17_Chr2g0292741 36.923 520 300 13 35 542 32 535 5.68e-104 323
Msa0543850 MtrunA17_Chr7g0224491 45.768 319 138 9 235 543 1 294 5.12e-82 258
Msa0543850 MtrunA17_Chr7g0224451 43.624 298 148 7 51 337 150 438 4.89e-76 247
Msa0543850 MtrunA17_Chr2g0292761 32.996 494 296 11 55 542 76 540 4.36e-75 248
Msa0543850 MtrunA17_Chr2g0319671 46.784 171 87 1 113 283 102 268 6.36e-49 171
Msa0543850 MtrunA17_Chr7g0224481 41.146 192 101 5 51 232 150 339 3.57e-37 140
Msa0543850 MtrunA17_Chr2g0319691 51.261 119 58 0 175 293 1 119 9.28e-36 130
Msa0543850 MtrunA17_Chr2g0319681 34.335 233 146 3 317 546 1 229 1.85e-34 130
Msa0543850 MtrunA17_Chr2g0319701 36.245 229 137 5 321 546 5 227 2.01e-34 130
Msa0543850 MtrunA17_Chr7g0224461 45.902 122 50 4 431 543 1 115 1.11e-21 90.5
Msa0543850 MtrunA17_Chr2g0304191 31.618 136 83 3 69 199 1 131 5.19e-16 75.1
Msa0543850 MtrunA17_Chr7g0224431 34.375 160 64 4 84 242 1 120 1.19e-15 73.6
Msa0543850 MtrunA17_Chr1g0205981 51.852 81 35 2 40 120 112 188 1.93e-15 75.1
Msa0543850 MtrunA17_Chr4g0028991 23.046 499 281 19 119 543 212 681 8.29e-14 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0543850 AT1G05230.8 77.273 550 104 5 1 547 190 721 0.0 855
Msa0543850 AT1G05230.4 77.273 550 104 5 1 547 190 721 0.0 855
Msa0543850 AT1G05230.9 77.273 550 104 5 1 547 190 721 0.0 855
Msa0543850 AT1G05230.10 77.273 550 104 5 1 547 190 721 0.0 855
Msa0543850 AT1G05230.7 77.273 550 104 5 1 547 190 721 0.0 855
Msa0543850 AT1G05230.11 77.273 550 104 5 1 547 190 721 0.0 855
Msa0543850 AT1G05230.2 77.273 550 104 5 1 547 190 721 0.0 855
Msa0543850 AT1G05230.6 77.273 550 104 5 1 547 190 721 0.0 855
Msa0543850 AT1G05230.5 77.273 550 104 5 1 547 190 721 0.0 855
Msa0543850 AT1G05230.1 77.273 550 104 5 1 547 190 721 0.0 855
Msa0543850 AT1G05230.3 76.909 550 104 6 1 547 190 719 0.0 845
Msa0543850 AT4G04890.2 75.451 554 120 9 1 543 188 736 0.0 830
Msa0543850 AT4G04890.1 75.451 554 120 9 1 543 188 736 0.0 830
Msa0543850 AT4G21750.2 72.213 583 118 8 1 547 188 762 0.0 824
Msa0543850 AT4G21750.1 72.213 583 118 8 1 547 188 762 0.0 824
Msa0543850 AT4G21750.3 72.213 583 118 8 1 547 188 762 0.0 824
Msa0543850 AT4G21750.4 72.213 583 118 8 1 547 188 762 0.0 824
Msa0543850 AT1G05230.12 76.235 425 87 4 1 422 190 603 0.0 665
Msa0543850 AT3G61150.1 53.519 540 233 7 17 546 276 807 0.0 560
Msa0543850 AT2G32370.1 53.452 507 218 7 37 539 225 717 0.0 545
Msa0543850 AT4G00730.1 56.504 492 203 7 58 546 319 802 0.0 537
Msa0543850 AT1G73360.1 45.009 571 280 12 1 547 158 718 1.96e-151 451
Msa0543850 AT1G17920.2 43.816 566 265 12 1 543 147 682 2.71e-147 439
Msa0543850 AT1G17920.1 43.816 566 265 12 1 543 147 682 2.71e-147 439
Msa0543850 AT5G52170.2 45.122 492 233 7 60 542 220 683 1.04e-139 420
Msa0543850 AT1G79840.3 42.115 520 279 7 34 545 182 687 6.09e-139 418
Msa0543850 AT1G79840.6 42.115 520 279 7 34 545 182 687 6.09e-139 418
Msa0543850 AT1G79840.1 42.115 520 279 7 34 545 240 745 1.91e-138 419
Msa0543850 AT5G52170.3 45.325 492 227 9 60 542 220 678 4.62e-138 416
Msa0543850 AT5G52170.1 45.325 492 227 9 60 542 220 678 4.62e-138 416
Msa0543850 AT1G79840.2 42.115 520 279 7 34 545 269 774 1.38e-137 417
Msa0543850 AT5G46880.3 38.422 583 311 11 1 544 235 808 6.01e-131 402
Msa0543850 AT5G46880.1 38.422 583 311 11 1 544 235 808 6.01e-131 402
Msa0543850 AT5G46880.2 38.422 583 311 11 1 544 235 808 6.01e-131 402
Msa0543850 AT4G17710.1 41.903 494 261 9 55 542 230 703 1.94e-123 379
Msa0543850 AT4G25530.1 40.650 492 274 7 54 542 206 682 1.84e-112 350
Msa0543850 AT1G79840.4 42.857 392 206 5 34 418 240 620 4.84e-107 334
Msa0543850 AT3G03260.1 38.386 508 277 13 55 543 205 695 1.04e-104 330
Msa0543850 AT5G17320.1 35.842 505 293 15 54 545 232 718 5.48e-87 284
Msa0543850 AT1G34650.1 35.547 512 305 14 46 545 210 708 1.10e-82 272
Msa0543850 AT4G00730.2 50.413 242 114 3 58 297 319 556 3.00e-76 252
Msa0543850 AT3G03260.2 37.537 341 201 9 55 390 205 538 5.75e-65 221
Msa0543850 AT1G79840.5 41.697 271 140 5 34 297 240 499 2.80e-62 213
Msa0543850 AT5G07260.1 29.953 424 254 16 68 479 99 491 6.79e-42 158
Msa0543850 AT5G07260.2 29.787 423 251 16 68 479 99 486 2.15e-41 157
Msa0543850 AT4G26920.1 23.191 470 253 18 69 522 72 449 2.51e-16 82.0
Msa0543850 AT4G32880.1 21.912 502 286 18 119 546 205 674 1.41e-14 77.4
Msa0543850 AT1G52150.1 22.424 495 286 19 119 543 206 672 6.80e-13 72.0
Msa0543850 AT1G52150.3 22.424 495 286 19 119 543 206 672 6.97e-13 72.0
Msa0543850 AT1G52150.2 22.379 496 286 19 119 543 206 673 9.46e-13 71.6

Find 179 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATACTCTTCCTCCCTTCT+TGG 0.195793 4_1:-31228326 None:intergenic
CTCCCACCGCGTCCAGAAAT+TGG 0.236347 4_1:+31226206 Msa0543850:CDS
TACCCTATCAAAGTTTCTTT+TGG 0.243078 4_1:+31230346 Msa0543850:three_prime_UTR
TTGAGCTTCAAGACTAATAA+TGG 0.252984 4_1:+31229404 Msa0543850:three_prime_UTR
TTGCAATGTGCGCCATTTCT+TGG 0.253615 4_1:-31228712 None:intergenic
GGGTTGCCAAAACCAATTTC+TGG 0.255941 4_1:-31226218 None:intergenic
AGGTGGTGAGGAACTTAATT+AGG 0.263635 4_1:+31230194 Msa0543850:three_prime_UTR
ATGTTGCTCTGGCTCGAATT+TGG 0.272485 4_1:-31229029 None:intergenic
CCGCGTCCAGAAATTGGTTT+TGG 0.273136 4_1:+31226212 Msa0543850:CDS
TTAAAGCAATGGTGTGTCTT+AGG 0.281630 4_1:-31230497 None:intergenic
GCAGCTATGGAAGAGCTTAT+TGG 0.288607 4_1:+31226347 Msa0543850:CDS
TTTCTAAGAGGATAATTATA+AGG 0.293415 4_1:+31230266 Msa0543850:three_prime_UTR
CTCAAATGGGTGATCCTCTT+TGG 0.301795 4_1:+31226375 Msa0543850:CDS
CAGCTATGGAAGAGCTTATT+GGG 0.306565 4_1:+31226348 Msa0543850:CDS
CATTTAAAACCATTAGGTTT+AGG 0.310632 4_1:-31226476 None:intergenic
ACAGCTAACTCTATTATTAT+CGG 0.326960 4_1:-31226323 None:intergenic
AGTCCAAAAGAAACTTTGAT+AGG 0.331169 4_1:-31230349 None:intergenic
TTCTAAAACACATAAATGAT+AGG 0.340314 4_1:-31230446 None:intergenic
GGCAGTTGTGGATGTGTCTT+TGG 0.341878 4_1:+31227284 Msa0543850:CDS
TGAAGAGGTTGCATTGCTTA+TGG 0.350980 4_1:+31230158 Msa0543850:three_prime_UTR
CCCACAAGGGATTGGTACTA+TGG 0.352325 4_1:+31226238 Msa0543850:CDS
ATCGACTTACATTTACTCTA+AGG 0.352335 4_1:-31228766 None:intergenic
ATTGCAAATGGACGTGATAC+TGG 0.354415 4_1:+31228728 Msa0543850:CDS
AAAACTTGTGCTGGCGGAAC+AGG 0.361955 4_1:-31228531 None:intergenic
GCTATGAATGTAGTTCTAAA+TGG 0.363093 4_1:+31229122 Msa0543850:CDS
AAGAGGATAATTATAAGGTT+CGG 0.366136 4_1:+31230271 Msa0543850:three_prime_UTR
TAGGCTTAATTTACAAGAAT+GGG 0.368062 4_1:+31230213 Msa0543850:three_prime_UTR
GGAAGAAGTGCTACATAATC+TGG 0.371226 4_1:-31229152 None:intergenic
AAAATACCAGGAGGTCTTCC+AGG 0.377607 4_1:-31228480 None:intergenic
TTAGGCTTAATTTACAAGAA+TGG 0.384657 4_1:+31230212 Msa0543850:three_prime_UTR
AATACCTTTGAGTAACCATT+TGG 0.385606 4_1:-31227375 None:intergenic
TATCTATGCACCGGTAGATA+TGG 0.387839 4_1:+31229097 Msa0543850:CDS
AGATGTAGAAGAAGGCCTTC+TGG 0.388784 4_1:+31227333 Msa0543850:CDS
AGAGGATAATTATAAGGTTC+GGG 0.389858 4_1:+31230272 Msa0543850:three_prime_UTR
CTCGCGAGAGTTACTTTGTA+AGG 0.396305 4_1:+31227232 Msa0543850:CDS
TGGACTAATTAGATATGGTT+AGG 0.396599 4_1:+31230366 Msa0543850:three_prime_UTR
AGGTCGTTTCCTCGTGGGAT+TGG 0.405874 4_1:+31226452 Msa0543850:CDS
GCTTCACATTTAAAACCATT+AGG 0.407416 4_1:-31226482 None:intergenic
GCTATCCTCCCTGATGGAAA+TGG 0.410049 4_1:+31229185 Msa0543850:CDS
CAATTCTATTGCAGTTTACA+AGG 0.413860 4_1:+31230134 Msa0543850:intron
CTCCCTGATGGAAATGGAAA+TGG 0.415022 4_1:+31229191 Msa0543850:CDS
GTTAGCACTGGACATGCATT+TGG 0.416621 4_1:+31228030 Msa0543850:CDS
CTACATCTAGATGAAGGACT+AGG 0.422205 4_1:-31227318 None:intergenic
GCTTATTGGGATGGCTCAAA+TGG 0.425134 4_1:+31226361 Msa0543850:CDS
TATTATCGGTTTATCTGCTT+CGG 0.425362 4_1:-31226309 None:intergenic
TGCGGTGAAACTGGCGGAGG+AGG 0.426052 4_1:+31229218 Msa0543850:CDS
CAATGGTCAACGGTGTTTGC+TGG 0.428126 4_1:+31226790 Msa0543850:CDS
TATGTAGCACTTCTTCCATC+AGG 0.428228 4_1:+31229158 Msa0543850:CDS
AATGGAGTCTGCGGTGAAAC+TGG 0.430513 4_1:+31229209 Msa0543850:CDS
CTTGCAAAATCCTTTCTAAG+AGG 0.434630 4_1:+31230254 Msa0543850:three_prime_UTR
CCAACTGCTAAACTCTCTCT+TGG 0.436250 4_1:+31229302 Msa0543850:CDS
GTTTAAACCTCACATAATAC+TGG 0.440805 4_1:-31230475 None:intergenic
GTAGTATTAACATGTTGCTC+TGG 0.442265 4_1:-31229040 None:intergenic
GGGATTGGACCTAAACCTAA+TGG 0.443923 4_1:+31226467 Msa0543850:CDS
AACAACATCCTGACGGGACA+TGG 0.445961 4_1:+31227262 Msa0543850:CDS
ACTCCATTTCCATTTCCATC+AGG 0.446625 4_1:-31229194 None:intergenic
GTGTTGTCAACTGGAGTGGC+AGG 0.446857 4_1:+31226841 Msa0543850:CDS
GGAGGAGGAGGAGCTGGTGC+TGG 0.447028 4_1:+31229233 Msa0543850:CDS
GAGGAAGAGTATGTTGAAAC+TGG 0.447997 4_1:+31228335 Msa0543850:CDS
CAAAGTAACTCTCGCGAGTT+GGG 0.448284 4_1:-31227227 None:intergenic
AGAATCAAAGCTTCTTTATC+CGG 0.448371 4_1:+31229368 Msa0543850:CDS
CACACATGGACAACACTGTC+TGG 0.450880 4_1:+31228408 Msa0543850:CDS
CGTAAGAGTGTCGATGATCC+TGG 0.451139 4_1:+31228462 Msa0543850:CDS
CGGTGGAGTTGTCCAAGAAA+TGG 0.457380 4_1:+31228700 Msa0543850:CDS
TGCATTTGGTGCAAAACGTT+GGG 0.460920 4_1:+31228044 Msa0543850:CDS
ACAGTGGAGGTGTTGTCAAC+TGG 0.461190 4_1:+31226832 Msa0543850:CDS
AGAGTTAGCTGTTGCAGCTA+TGG 0.461321 4_1:+31226334 Msa0543850:CDS
TTATAATTATCCTCTTAGAA+AGG 0.464421 4_1:-31230264 None:intergenic
GAAATGGCGCACATTGCAAA+TGG 0.473589 4_1:+31228716 Msa0543850:CDS
ACTGGCGGAGGAGGAGGAGC+TGG 0.473807 4_1:+31229227 Msa0543850:CDS
TGGACAACACTGTCTGGAAC+AGG 0.477040 4_1:+31228414 Msa0543850:CDS
GGAGGAGGAGCTGGTGCTGG+TGG 0.478826 4_1:+31229236 Msa0543850:CDS
CCATAGTACCAATCCCTTGT+GGG 0.479347 4_1:-31226238 None:intergenic
GGTGAAACTGGCGGAGGAGG+AGG 0.480687 4_1:+31229221 Msa0543850:CDS
GAAACTTTGATAGGGTAGTA+AGG 0.485765 4_1:-31230340 None:intergenic
CTCCATTTCCATTTCCATCA+GGG 0.486392 4_1:-31229193 None:intergenic
TTAATGAAGATGAATATGTT+AGG 0.488862 4_1:+31226432 Msa0543850:CDS
ACAACTGCCCATGTCCCGTC+AGG 0.494676 4_1:-31227270 None:intergenic
TTTGGCAACCCACAAGGGAT+TGG 0.494683 4_1:+31226230 Msa0543850:CDS
CTCAACATTTAATATACCTA+GGG 0.494847 4_1:-31230305 None:intergenic
GATATTCTACGGTCAATTAG+TGG 0.502137 4_1:+31226281 Msa0543850:CDS
ATGTTTGAGTAGTTTACCAC+TGG 0.510620 4_1:-31226173 None:intergenic
ATTGGTACTATGGACATGTA+TGG 0.511477 4_1:+31226248 Msa0543850:CDS
ATGTTAGGTCGTTTCCTCGT+GGG 0.513468 4_1:+31226447 Msa0543850:CDS
AGCACAGTAGCAGCTCCATC+AGG 0.513971 4_1:-31226410 None:intergenic
CTCATTTGTTATCTATGCAC+CGG 0.516034 4_1:+31229088 Msa0543850:CDS
GAGAGTGTAACAGATGCAAC+AGG 0.516631 4_1:+31229065 Msa0543850:CDS
TTAATTCAAGAGATGCCAAA+TGG 0.518826 4_1:+31227360 Msa0543850:CDS
GCCTAAGCTAAACAAAAGAC+TGG 0.523065 4_1:-31230390 None:intergenic
GTCCAAAAGAAACTTTGATA+GGG 0.525625 4_1:-31230348 None:intergenic
CTCTCGCGAGTTGGGACAAG+AGG 0.526640 4_1:-31227219 None:intergenic
CAGCAAACACCGTTGACCAT+TGG 0.528564 4_1:-31226789 None:intergenic
TACAAGCAGATTGTTAGCAC+TGG 0.528579 4_1:+31228018 Msa0543850:CDS
TCTAAAACACATAAATGATA+GGG 0.529780 4_1:-31230445 None:intergenic
ATATATACCGCCAACATCTA+CGG 0.532826 4_1:-31228127 None:intergenic
GGTGGGAATGTTTGTTGCCA+TGG 0.532843 4_1:-31228106 None:intergenic
ACAAAGTAACTCTCGCGAGT+TGG 0.537069 4_1:-31227228 None:intergenic
CAAAATACGTCAATATCACT+TGG 0.538257 4_1:-31230562 None:intergenic
GTTGAAACTGGCAGAGAGAA+TGG 0.540573 4_1:+31228347 Msa0543850:CDS
TTTGAAAAGCGACGGTCAGA+AGG 0.541068 4_1:-31229262 None:intergenic
CCAAAACCAATTTCTGGACG+CGG 0.541993 4_1:-31226212 None:intergenic
CTTATTGGGATGGCTCAAAT+GGG 0.542298 4_1:+31226362 Msa0543850:CDS
TGCAGTTTACAAGGATGAAG+AGG 0.543194 4_1:+31230143 Msa0543850:intron
GAGCCAGTGGCGATATTCTA+CGG 0.544854 4_1:+31226270 Msa0543850:CDS
CTTCATCTAGATGTAGAAGA+AGG 0.545581 4_1:+31227325 Msa0543850:CDS
GATGATCCTGGAAGACCTCC+TGG 0.545888 4_1:+31228474 Msa0543850:CDS
TAGTGATAACAAACCAAGAA+GGG 0.547679 4_1:+31228313 Msa0543850:intron
TCTGGACGCGGTGGGAGAGA+TGG 0.548949 4_1:-31226200 None:intergenic
TTTCCAACATATAGGTGACA+TGG 0.550283 4_1:+31227953 Msa0543850:intron
TTAGTGATAACAAACCAAGA+AGG 0.552241 4_1:+31228312 Msa0543850:intron
CTTCTTCTACATCTAGATGA+AGG 0.552711 4_1:-31227324 None:intergenic
GCTCAACATTTAATATACCT+AGG 0.554111 4_1:-31230306 None:intergenic
TGGTTAAGTACTACACCTGA+TGG 0.558663 4_1:+31226395 Msa0543850:CDS
CGAAAGGCTTGCTAGCTCCA+TGG 0.560399 4_1:+31228089 Msa0543850:CDS
CAATGGCAGCGAAGTACGTA+GGG 0.564217 4_1:+31226147 None:intergenic
ATATTCTACGGTCAATTAGT+GGG 0.565591 4_1:+31226282 Msa0543850:CDS
ATGCATTTGGTGCAAAACGT+TGG 0.567718 4_1:+31228043 Msa0543850:CDS
CTTTAGATCGACAATGCGAA+AGG 0.567913 4_1:+31228073 Msa0543850:CDS
TTCATAGCAACCATATCTAC+CGG 0.568044 4_1:-31229107 None:intergenic
ATCGGTTTATCTGCTTCGGT+CGG 0.568358 4_1:-31226305 None:intergenic
TGACGGGACATGGGCAGTTG+TGG 0.568646 4_1:+31227272 Msa0543850:CDS
CAACCTTGTTGAGATTCTCA+TGG 0.570569 4_1:+31226541 Msa0543850:CDS
TCCATAGTACCAATCCCTTG+TGG 0.571179 4_1:-31226239 None:intergenic
CAGTGGGATATTCTTTCTAA+CGG 0.572964 4_1:+31228680 Msa0543850:intron
AACATTCCCACCGTAGATGT+TGG 0.573798 4_1:+31228117 Msa0543850:CDS
ATAATGAGAAATTAAAGCAA+TGG 0.575101 4_1:-31230508 None:intergenic
CCAAGAGAGAGTTTAGCAGT+TGG 0.575737 4_1:-31229302 None:intergenic
GGGAGGATAGCAAATCCTGA+TGG 0.577076 4_1:-31229173 None:intergenic
TTCCAACATATAGGTGACAT+GGG 0.577979 4_1:+31227954 Msa0543850:intron
TGTTGAGATTCTCATGGATG+TGG 0.578448 4_1:+31226547 Msa0543850:CDS
TGTTTCAAGAGCTGTGACAG+TGG 0.585128 4_1:+31226816 Msa0543850:CDS
GCTCAACTAAGCACTTTCAC+CGG 0.585345 4_1:-31229387 None:intergenic
AACATGTAGAAGTGGATGAG+AGG 0.585664 4_1:+31227980 Msa0543850:CDS
TACATCTAGATGAAGGACTA+GGG 0.586817 4_1:-31227317 None:intergenic
GGATTTGCTATCCTCCCTGA+TGG 0.589528 4_1:+31229179 Msa0543850:CDS
TATGTTAGGTCGTTTCCTCG+TGG 0.589599 4_1:+31226446 Msa0543850:CDS
AACCAAGATTTGAAAAGCGA+CGG 0.590397 4_1:-31229270 None:intergenic
AATACGTCAATATCACTTGG+AGG 0.592070 4_1:-31230559 None:intergenic
ACAACATCCTGACGGGACAT+GGG 0.595244 4_1:+31227263 Msa0543850:CDS
GTGCTTCAACAGCACACACA+TGG 0.598437 4_1:+31228394 Msa0543850:CDS
ATTGTAAACAACATCCTGAC+GGG 0.600101 4_1:+31227256 Msa0543850:CDS
GATGCCAAATGGTTACTCAA+AGG 0.601554 4_1:+31227371 Msa0543850:CDS
GTCTGCGGTGAAACTGGCGG+AGG 0.606388 4_1:+31229215 Msa0543850:CDS
TGACCGTAGAATATCGCCAC+TGG 0.607941 4_1:-31226273 None:intergenic
TATGGAAGAGCTTATTGGGA+TGG 0.611739 4_1:+31226352 Msa0543850:CDS
AGTCATCACTCTCACATCAT+CGG 0.612975 4_1:-31228439 None:intergenic
ACATGTAGAAGTGGATGAGA+GGG 0.614639 4_1:+31227981 Msa0543850:CDS
TTGAAAAGCGACGGTCAGAA+GGG 0.614971 4_1:-31229261 None:intergenic
ATTCCCACCGTAGATGTTGG+CGG 0.617429 4_1:+31228120 Msa0543850:CDS
CTACAATGGTGCTTTACAAG+TGG 0.618320 4_1:+31226867 Msa0543850:CDS
AAACCAATTTCTGGACGCGG+TGG 0.619643 4_1:-31226209 None:intergenic
TCTTGAATTAAGCAACCAGA+AGG 0.628017 4_1:-31227348 None:intergenic
CGAGTTGGGACAAGAGGTGA+AGG 0.631686 4_1:-31227213 None:intergenic
TTCGGGAATTGACTTCCCCT+AGG 0.632649 4_1:+31230289 Msa0543850:three_prime_UTR
AACCAATTTCTGGACGCGGT+GGG 0.632731 4_1:-31226208 None:intergenic
ATGGGTTGAACATGTAGAAG+TGG 0.635474 4_1:+31227972 Msa0543850:CDS
ATGAATGTAGTTCTAAATGG+AGG 0.639925 4_1:+31229125 Msa0543850:CDS
TATTGTAAACAACATCCTGA+CGG 0.640849 4_1:+31227255 Msa0543850:CDS
ACAATGGCAGCGAAGTACGT+AGG 0.641043 4_1:+31226146 None:intergenic
TGTTGTCAACTGGAGTGGCA+GGG 0.641410 4_1:+31226842 Msa0543850:CDS
TATACCGCCAACATCTACGG+TGG 0.647103 4_1:-31228124 None:intergenic
TGGGATATTCTTTCTAACGG+TGG 0.650199 4_1:+31228683 Msa0543850:CDS
GGAGTCTGCGGTGAAACTGG+CGG 0.650477 4_1:+31229212 Msa0543850:CDS
ATACCGCCAACATCTACGGT+GGG 0.654303 4_1:-31228123 None:intergenic
GGAGGTGTTGTCAACTGGAG+TGG 0.656092 4_1:+31226837 Msa0543850:CDS
GGTGTAGTACTTAACCAAAG+AGG 0.657817 4_1:-31226389 None:intergenic
ATGGACATGTATGGAGCCAG+TGG 0.659051 4_1:+31226257 Msa0543850:CDS
TGATAACAAACCAAGAAGGG+AGG 0.664902 4_1:+31228316 Msa0543850:CDS
CATTTCCATTTCCATCAGGG+AGG 0.666915 4_1:-31229190 None:intergenic
TTCAAGAGCTGTGACAGTGG+AGG 0.672894 4_1:+31226819 Msa0543850:CDS
AGAGGGACCAGTATTATGTG+AGG 0.677452 4_1:+31230468 Msa0543850:three_prime_UTR
GGAGTGGCAGGGAACTACAA+TGG 0.679417 4_1:+31226853 Msa0543850:CDS
TAACGAGAGCTCCAGAACTG+AGG 0.682761 4_1:+31228566 Msa0543850:CDS
CATCCATGAGAATCTCAACA+AGG 0.687534 4_1:-31226544 None:intergenic
GGAAATGGAAATGGAGTCTG+CGG 0.694782 4_1:+31229200 Msa0543850:CDS
CATGTAGAAGTGGATGAGAG+GGG 0.694795 4_1:+31227982 Msa0543850:CDS
TCAACATTTAATATACCTAG+GGG 0.714596 4_1:-31230304 None:intergenic
GAAGTACGTAGGGAAGCCAG+TGG 0.754472 4_1:+31226157 Msa0543850:CDS
GGTTTAGGTCCAATCCCACG+AGG 0.756148 4_1:-31226461 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAGTAATATATATATA+TGG - chr4_1:31226609-31226628 None:intergenic 10.0%
!!! TTGTTATATCATAAATTTTA+AGG - chr4_1:31228275-31228294 None:intergenic 10.0%
!! ATCATAATAATCAAGATATT+TGG + chr4_1:31227822-31227841 Msa0543850:intron 15.0%
!! TCAAATAGAAAATATAAACA+TGG - chr4_1:31228950-31228969 None:intergenic 15.0%
!!! AAATTTGAGATAAATCATTA+TGG + chr4_1:31229499-31229518 Msa0543850:intron 15.0%
!!! GCTAGTTTTTTTTTTTTTTA+AGG - chr4_1:31230041-31230060 None:intergenic 15.0%
!! TTAATGAAGATGAATATGTT+AGG + chr4_1:31226432-31226451 Msa0543850:CDS 20.0%
!! TGAATTTAACTGTGTATATA+TGG + chr4_1:31226637-31226656 Msa0543850:intron 20.0%
!!! ATTTTTTCTGTCTCTAATTT+CGG + chr4_1:31227029-31227048 Msa0543850:intron 20.0%
!! CTATTTGACATATTAATAAG+AGG - chr4_1:31227089-31227108 None:intergenic 20.0%
!! TCAAATAGTTATAGTTAACT+TGG + chr4_1:31227101-31227120 Msa0543850:intron 20.0%
!!! ATTAATGTTTTCCAACATAT+AGG + chr4_1:31227945-31227964 Msa0543850:intron 20.0%
!! ATATAACAAGTGTTATAATG+TGG + chr4_1:31228286-31228305 Msa0543850:intron 20.0%
!! GAAAACATAAGAAAAACTAA+GGG - chr4_1:31228622-31228641 None:intergenic 20.0%
!! AGAAAACATAAGAAAAACTA+AGG - chr4_1:31228623-31228642 None:intergenic 20.0%
!! AACTGGAAAATCTTTAATAT+AGG - chr4_1:31229461-31229480 None:intergenic 20.0%
!!! ACTTTTATAATCGAGTTATA+GGG + chr4_1:31229902-31229921 Msa0543850:intron 20.0%
!! CTTATGGAAAAAAAATCAAA+AGG + chr4_1:31230174-31230193 Msa0543850:three_prime_UTR 20.0%
!! TTATAATTATCCTCTTAGAA+AGG - chr4_1:31230267-31230286 None:intergenic 20.0%
!!! TTTCTAAGAGGATAATTATA+AGG + chr4_1:31230266-31230285 Msa0543850:three_prime_UTR 20.0%
!!! TCTAAAACACATAAATGATA+GGG - chr4_1:31230448-31230467 None:intergenic 20.0%
!!! TTCTAAAACACATAAATGAT+AGG - chr4_1:31230449-31230468 None:intergenic 20.0%
!! ATAATGAGAAATTAAAGCAA+TGG - chr4_1:31230511-31230530 None:intergenic 20.0%
!!! TCATTATAACTCTTTTGAAA+TGG + chr4_1:31230524-31230543 Msa0543850:three_prime_UTR 20.0%
! ACAGCTAACTCTATTATTAT+CGG - chr4_1:31226326-31226345 None:intergenic 25.0%
!! CATTTAAAACCATTAGGTTT+AGG - chr4_1:31226479-31226498 None:intergenic 25.0%
! ATACACAGTTAAATTCACAT+GGG - chr4_1:31226634-31226653 None:intergenic 25.0%
! TATACACAGTTAAATTCACA+TGG - chr4_1:31226635-31226654 None:intergenic 25.0%
!!! TGATTTTAGTTCAATCAGTA+AGG + chr4_1:31226927-31226946 Msa0543850:intron 25.0%
! TTTGCAAACTTTCATTTGAA+GGG - chr4_1:31227580-31227599 None:intergenic 25.0%
! GAAAGTTTGCAAAGTAAAAA+TGG + chr4_1:31227587-31227606 Msa0543850:intron 25.0%
!! TAAATCTAAGTGATGATGTT+TGG - chr4_1:31227615-31227634 None:intergenic 25.0%
! TTTCTAAGTGATAAGTTGTT+TGG - chr4_1:31227776-31227795 None:intergenic 25.0%
! ATAAGAAAAACTAAGGGAAA+TGG - chr4_1:31228616-31228635 None:intergenic 25.0%
!!! AAGACTAATAATGGATTTTG+AGG + chr4_1:31229413-31229432 Msa0543850:three_prime_UTR 25.0%
!! CTTTTATATTGCACTAAATG+CGG + chr4_1:31229628-31229647 Msa0543850:intron 25.0%
! AAAAACACATACATCTAACA+TGG - chr4_1:31229696-31229715 None:intergenic 25.0%
! GTAGTACTTCTTAAATATCA+AGG - chr4_1:31229724-31229743 None:intergenic 25.0%
!! GACTTTTATAATCGAGTTAT+AGG + chr4_1:31229901-31229920 Msa0543850:intron 25.0%
!! CTTTTATAATCGAGTTATAG+GGG + chr4_1:31229903-31229922 Msa0543850:intron 25.0%
! ATGGAAAAAAAATCAAAAGG+TGG + chr4_1:31230177-31230196 Msa0543850:three_prime_UTR 25.0%
! TTAGGCTTAATTTACAAGAA+TGG + chr4_1:31230212-31230231 Msa0543850:three_prime_UTR 25.0%
! TAGGCTTAATTTACAAGAAT+GGG + chr4_1:31230213-31230232 Msa0543850:three_prime_UTR 25.0%
! AAGAGGATAATTATAAGGTT+CGG + chr4_1:31230271-31230290 Msa0543850:three_prime_UTR 25.0%
! TCAACATTTAATATACCTAG+GGG - chr4_1:31230307-31230326 None:intergenic 25.0%
! CTCAACATTTAATATACCTA+GGG - chr4_1:31230308-31230327 None:intergenic 25.0%
!! TCTTTTGGACTAATTAGATA+TGG + chr4_1:31230361-31230380 Msa0543850:three_prime_UTR 25.0%
!!! TCATTTATGTGTTTTAGAAG+AGG + chr4_1:31230450-31230469 Msa0543850:three_prime_UTR 25.0%
!!! CATTTATGTGTTTTAGAAGA+GGG + chr4_1:31230451-31230470 Msa0543850:three_prime_UTR 25.0%
ATATTCTACGGTCAATTAGT+GGG + chr4_1:31226282-31226301 Msa0543850:CDS 30.0%
!! TATTATCGGTTTATCTGCTT+CGG - chr4_1:31226312-31226331 None:intergenic 30.0%
! GCTTCACATTTAAAACCATT+AGG - chr4_1:31226485-31226504 None:intergenic 30.0%
! TTTTAAATGTGAAGCTTCGA+GGG + chr4_1:31226490-31226509 Msa0543850:CDS 30.0%
! CGATATTGCAATCTAAAACA+TGG + chr4_1:31226693-31226712 Msa0543850:intron 30.0%
!!! TTTTTGCCTTTTAGAACCAA+TGG + chr4_1:31226773-31226792 Msa0543850:intron 30.0%
TATTGTAAACAACATCCTGA+CGG + chr4_1:31227255-31227274 Msa0543850:CDS 30.0%
TTAATTCAAGAGATGCCAAA+TGG + chr4_1:31227360-31227379 Msa0543850:CDS 30.0%
AATACCTTTGAGTAACCATT+TGG - chr4_1:31227378-31227397 None:intergenic 30.0%
!!! TACATTTTAAAGCGATGATG+TGG - chr4_1:31227456-31227475 None:intergenic 30.0%
! TTTAAAATGTAGCCTTCGTT+TGG + chr4_1:31227465-31227484 Msa0543850:intron 30.0%
TTGCAAACTTTCATTTGAAG+GGG - chr4_1:31227579-31227598 None:intergenic 30.0%
CTTTGCAAACTTTCATTTGA+AGG - chr4_1:31227581-31227600 None:intergenic 30.0%
TTAGTGATAACAAACCAAGA+AGG + chr4_1:31228312-31228331 Msa0543850:intron 30.0%
TAGTGATAACAAACCAAGAA+GGG + chr4_1:31228313-31228332 Msa0543850:intron 30.0%
! AGAATGGTGATAAGTTTTTG+TGG + chr4_1:31228363-31228382 Msa0543850:CDS 30.0%
AGAAATTCAAAAACTTGTGC+TGG - chr4_1:31228543-31228562 None:intergenic 30.0%
GGGAAATGGATATTAGTAAT+TGG - chr4_1:31228602-31228621 None:intergenic 30.0%
TTTAACCTCTTAAATGACAG+TGG + chr4_1:31228663-31228682 Msa0543850:intron 30.0%
TTAACCTCTTAAATGACAGT+GGG + chr4_1:31228664-31228683 Msa0543850:intron 30.0%
! ATCGACTTACATTTACTCTA+AGG - chr4_1:31228769-31228788 None:intergenic 30.0%
TGCTGCAAACATTATTATTG+TGG + chr4_1:31228818-31228837 Msa0543850:intron 30.0%
! TTTGACAATCTGAAACTTAC+TGG + chr4_1:31228965-31228984 Msa0543850:intron 30.0%
TGAAACTTACTGGTGAATTA+AGG + chr4_1:31228975-31228994 Msa0543850:intron 30.0%
GAAACTTACTGGTGAATTAA+GGG + chr4_1:31228976-31228995 Msa0543850:intron 30.0%
!! GCTATGAATGTAGTTCTAAA+TGG + chr4_1:31229122-31229141 Msa0543850:CDS 30.0%
!! ATGAATGTAGTTCTAAATGG+AGG + chr4_1:31229125-31229144 Msa0543850:CDS 30.0%
AGAATCAAAGCTTCTTTATC+CGG + chr4_1:31229368-31229387 Msa0543850:CDS 30.0%
TTGAGCTTCAAGACTAATAA+TGG + chr4_1:31229404-31229423 Msa0543850:three_prime_UTR 30.0%
CACTACAAATAACAACTAAC+TGG - chr4_1:31229478-31229497 None:intergenic 30.0%
AGAAACGTATGTTAGATACA+TGG + chr4_1:31229563-31229582 Msa0543850:intron 30.0%
! CATCAAATCATTTCGAGTAT+AGG + chr4_1:31229747-31229766 Msa0543850:intron 30.0%
! TGCTTTTATAGTCGAGTTAT+AGG + chr4_1:31229804-31229823 Msa0543850:intron 30.0%
! GCTTTTATAGTCGAGTTATA+GGG + chr4_1:31229805-31229824 Msa0543850:intron 30.0%
ACTACTTTCCTCAAATCATT+TGG + chr4_1:31229836-31229855 Msa0543850:intron 30.0%
! CTCAAATCATTTGGAGTATA+GGG + chr4_1:31229845-31229864 Msa0543850:intron 30.0%
! AAAACAGGAGTACAAAAGAT+AGG - chr4_1:31229960-31229979 None:intergenic 30.0%
CAATTCTATTGCAGTTTACA+AGG + chr4_1:31230134-31230153 Msa0543850:intron 30.0%
AAAAAAATCAAAAGGTGGTG+AGG + chr4_1:31230182-31230201 Msa0543850:three_prime_UTR 30.0%
AGAGGATAATTATAAGGTTC+GGG + chr4_1:31230272-31230291 Msa0543850:three_prime_UTR 30.0%
GCTCAACATTTAATATACCT+AGG - chr4_1:31230309-31230328 None:intergenic 30.0%
! TACCCTATCAAAGTTTCTTT+TGG + chr4_1:31230346-31230365 Msa0543850:three_prime_UTR 30.0%
GTCCAAAAGAAACTTTGATA+GGG - chr4_1:31230351-31230370 None:intergenic 30.0%
AGTCCAAAAGAAACTTTGAT+AGG - chr4_1:31230352-31230371 None:intergenic 30.0%
TGGACTAATTAGATATGGTT+AGG + chr4_1:31230366-31230385 Msa0543850:three_prime_UTR 30.0%
GTTTAAACCTCACATAATAC+TGG - chr4_1:31230478-31230497 None:intergenic 30.0%
CAAAATACGTCAATATCACT+TGG - chr4_1:31230565-31230584 None:intergenic 30.0%
ATGTTTGAGTAGTTTACCAC+TGG - chr4_1:31226176-31226195 None:intergenic 35.0%
ATTGGTACTATGGACATGTA+TGG + chr4_1:31226248-31226267 Msa0543850:CDS 35.0%
GATATTCTACGGTCAATTAG+TGG + chr4_1:31226281-31226300 Msa0543850:CDS 35.0%
!! GTTTTAAATGTGAAGCTTCG+AGG + chr4_1:31226489-31226508 Msa0543850:CDS 35.0%
AGATTGCAATATCGCAATTG+CGG - chr4_1:31226687-31226706 None:intergenic 35.0%
!!! CTTTTAGAACCAATGGTCAA+CGG + chr4_1:31226780-31226799 Msa0543850:intron 35.0%
ATTGTAAACAACATCCTGAC+GGG + chr4_1:31227256-31227275 Msa0543850:CDS 35.0%
TACATCTAGATGAAGGACTA+GGG - chr4_1:31227320-31227339 None:intergenic 35.0%
CTTCTTCTACATCTAGATGA+AGG - chr4_1:31227327-31227346 None:intergenic 35.0%
CTTCATCTAGATGTAGAAGA+AGG + chr4_1:31227325-31227344 Msa0543850:CDS 35.0%
TCTTGAATTAAGCAACCAGA+AGG - chr4_1:31227351-31227370 None:intergenic 35.0%
! TGTGGACAACTATTTTGCAT+TGG - chr4_1:31227438-31227457 None:intergenic 35.0%
GAGTGATACTGACATAATAG+TGG + chr4_1:31227689-31227708 Msa0543850:intron 35.0%
! TCAACCGCAATTTAGAACTA+TGG + chr4_1:31227749-31227768 Msa0543850:intron 35.0%
!! TGGACCATAGTTCTAAATTG+CGG - chr4_1:31227756-31227775 None:intergenic 35.0%
TTTCCAACATATAGGTGACA+TGG + chr4_1:31227953-31227972 Msa0543850:intron 35.0%
TTCCAACATATAGGTGACAT+GGG + chr4_1:31227954-31227973 Msa0543850:intron 35.0%
ATATATACCGCCAACATCTA+CGG - chr4_1:31228130-31228149 None:intergenic 35.0%
! ATGGTGATAAGTTTTTGTGG+TGG + chr4_1:31228366-31228385 Msa0543850:CDS 35.0%
!! TTAGTGCTGCTACTTCTTTT+TGG + chr4_1:31228505-31228524 Msa0543850:CDS 35.0%
AATTCAAAAACTTGTGCTGG+CGG - chr4_1:31228540-31228559 None:intergenic 35.0%
GAAAAACATTACCTCAGTTC+TGG - chr4_1:31228580-31228599 None:intergenic 35.0%
! ATATCCCACTGTCATTTAAG+AGG - chr4_1:31228671-31228690 None:intergenic 35.0%
CAGTGGGATATTCTTTCTAA+CGG + chr4_1:31228680-31228699 Msa0543850:intron 35.0%
ACGCAATGTAAAAGATTTCG+AGG - chr4_1:31228895-31228914 None:intergenic 35.0%
GTAGTATTAACATGTTGCTC+TGG - chr4_1:31229043-31229062 None:intergenic 35.0%
!! CTCATTTGTTATCTATGCAC+CGG + chr4_1:31229088-31229107 Msa0543850:CDS 35.0%
TTCATAGCAACCATATCTAC+CGG - chr4_1:31229110-31229129 None:intergenic 35.0%
AACCAAGATTTGAAAAGCGA+CGG - chr4_1:31229273-31229292 None:intergenic 35.0%
CCTATACTCCAAATGATTTG+AGG - chr4_1:31229847-31229866 None:intergenic 35.0%
! CCTCAAATCATTTGGAGTAT+AGG + chr4_1:31229844-31229863 Msa0543850:intron 35.0%
GTTAGTGTTTCGTCTATTGA+CGG + chr4_1:31229932-31229951 Msa0543850:intron 35.0%
! CTCTTAGAAAGGATTTTGCA+AGG - chr4_1:31230256-31230275 None:intergenic 35.0%
CTTGCAAAATCCTTTCTAAG+AGG + chr4_1:31230254-31230273 Msa0543850:three_prime_UTR 35.0%
GAAACTTTGATAGGGTAGTA+AGG - chr4_1:31230343-31230362 None:intergenic 35.0%
!! ACCAGTCTTTTGTTTAGCTT+AGG + chr4_1:31230389-31230408 Msa0543850:three_prime_UTR 35.0%
TTAAAGCAATGGTGTGTCTT+AGG - chr4_1:31230500-31230519 None:intergenic 35.0%
AATACGTCAATATCACTTGG+AGG - chr4_1:31230562-31230581 None:intergenic 35.0%
CAGCTATGGAAGAGCTTATT+GGG + chr4_1:31226348-31226367 Msa0543850:CDS 40.0%
TATGGAAGAGCTTATTGGGA+TGG + chr4_1:31226352-31226371 Msa0543850:CDS 40.0%
CTTATTGGGATGGCTCAAAT+GGG + chr4_1:31226362-31226381 Msa0543850:CDS 40.0%
GGTGTAGTACTTAACCAAAG+AGG - chr4_1:31226392-31226411 None:intergenic 40.0%
TGGTTAAGTACTACACCTGA+TGG + chr4_1:31226395-31226414 Msa0543850:CDS 40.0%
CAACCTTGTTGAGATTCTCA+TGG + chr4_1:31226541-31226560 Msa0543850:CDS 40.0%
CATCCATGAGAATCTCAACA+AGG - chr4_1:31226547-31226566 None:intergenic 40.0%
TGTTGAGATTCTCATGGATG+TGG + chr4_1:31226547-31226566 Msa0543850:CDS 40.0%
CAATTGCGGCTGTAATATGA+AGG - chr4_1:31226673-31226692 None:intergenic 40.0%
!! CGTTGACCATTGGTTCTAAA+AGG - chr4_1:31226782-31226801 None:intergenic 40.0%
CTACAATGGTGCTTTACAAG+TGG + chr4_1:31226867-31226886 Msa0543850:CDS 40.0%
TTCTGTGATAAGAGACTTCG+CGG + chr4_1:31226998-31227017 Msa0543850:intron 40.0%
CTACATCTAGATGAAGGACT+AGG - chr4_1:31227321-31227340 None:intergenic 40.0%
GATGCCAAATGGTTACTCAA+AGG + chr4_1:31227371-31227390 Msa0543850:CDS 40.0%
TTTACATTGACGCAGAAGCT+AGG + chr4_1:31227522-31227541 Msa0543850:intron 40.0%
!!! TTTGTCGCTTTTGAAACGCA+CGG + chr4_1:31227546-31227565 Msa0543850:intron 40.0%
TGCTGCGAACTTTGATATTG+CGG + chr4_1:31227658-31227677 Msa0543850:intron 40.0%
AACCCATGTCACCTATATGT+TGG - chr4_1:31227959-31227978 None:intergenic 40.0%
! ATGGGTTGAACATGTAGAAG+TGG + chr4_1:31227972-31227991 Msa0543850:CDS 40.0%
AACATGTAGAAGTGGATGAG+AGG + chr4_1:31227980-31227999 Msa0543850:CDS 40.0%
ACATGTAGAAGTGGATGAGA+GGG + chr4_1:31227981-31228000 Msa0543850:CDS 40.0%
TACAAGCAGATTGTTAGCAC+TGG + chr4_1:31228018-31228037 Msa0543850:CDS 40.0%
!! ATGCATTTGGTGCAAAACGT+TGG + chr4_1:31228043-31228062 Msa0543850:CDS 40.0%
!! TGCATTTGGTGCAAAACGTT+GGG + chr4_1:31228044-31228063 Msa0543850:CDS 40.0%
CTTTAGATCGACAATGCGAA+AGG + chr4_1:31228073-31228092 Msa0543850:CDS 40.0%
TGATAACAAACCAAGAAGGG+AGG + chr4_1:31228316-31228335 Msa0543850:CDS 40.0%
! GAGGAAGAGTATGTTGAAAC+TGG + chr4_1:31228335-31228354 Msa0543850:CDS 40.0%
AGTCATCACTCTCACATCAT+CGG - chr4_1:31228442-31228461 None:intergenic 40.0%
GCACTAAGAAAAATACCAGG+AGG - chr4_1:31228492-31228511 None:intergenic 40.0%
GCAGCACTAAGAAAAATACC+AGG - chr4_1:31228495-31228514 None:intergenic 40.0%
TGGGATATTCTTTCTAACGG+TGG + chr4_1:31228683-31228702 Msa0543850:CDS 40.0%
ATTGCAAATGGACGTGATAC+TGG + chr4_1:31228728-31228747 Msa0543850:CDS 40.0%
TGTTTGCAGCATAACTGCAT+CGG - chr4_1:31228809-31228828 None:intergenic 40.0%
! TATCTATGCACCGGTAGATA+TGG + chr4_1:31229097-31229116 Msa0543850:CDS 40.0%
!! GGAAGAAGTGCTACATAATC+TGG - chr4_1:31229155-31229174 None:intergenic 40.0%
TATGTAGCACTTCTTCCATC+AGG + chr4_1:31229158-31229177 Msa0543850:CDS 40.0%
CTCCATTTCCATTTCCATCA+GGG - chr4_1:31229196-31229215 None:intergenic 40.0%
ACTCCATTTCCATTTCCATC+AGG - chr4_1:31229197-31229216 None:intergenic 40.0%
TATAGTCGAGTTATAGGGCA+AGG + chr4_1:31229810-31229829 Msa0543850:intron 40.0%
AGAACACAAGAACGCAAAAC+AGG - chr4_1:31229975-31229994 None:intergenic 40.0%
! TGTTTTGCGTTCTTGTGTTC+TGG + chr4_1:31229974-31229993 Msa0543850:intron 40.0%
TGCAGTTTACAAGGATGAAG+AGG + chr4_1:31230143-31230162 Msa0543850:intron 40.0%
TGAAGAGGTTGCATTGCTTA+TGG + chr4_1:31230158-31230177 Msa0543850:three_prime_UTR 40.0%
AGGTGGTGAGGAACTTAATT+AGG + chr4_1:31230194-31230213 Msa0543850:three_prime_UTR 40.0%
GCCTAAGCTAAACAAAAGAC+TGG - chr4_1:31230393-31230412 None:intergenic 40.0%
! CCAAAACCAATTTCTGGACG+CGG - chr4_1:31226215-31226234 None:intergenic 45.0%
GGGTTGCCAAAACCAATTTC+TGG - chr4_1:31226221-31226240 None:intergenic 45.0%
!!! ATTGGTTTTGGCAACCCACA+AGG + chr4_1:31226224-31226243 Msa0543850:CDS 45.0%
!!! TTGGTTTTGGCAACCCACAA+GGG + chr4_1:31226225-31226244 Msa0543850:CDS 45.0%
CCATAGTACCAATCCCTTGT+GGG - chr4_1:31226241-31226260 None:intergenic 45.0%
TCCATAGTACCAATCCCTTG+TGG - chr4_1:31226242-31226261 None:intergenic 45.0%
! ATCGGTTTATCTGCTTCGGT+CGG - chr4_1:31226308-31226327 None:intergenic 45.0%
AGAGTTAGCTGTTGCAGCTA+TGG + chr4_1:31226334-31226353 Msa0543850:CDS 45.0%
GCAGCTATGGAAGAGCTTAT+TGG + chr4_1:31226347-31226366 Msa0543850:CDS 45.0%
GCTTATTGGGATGGCTCAAA+TGG + chr4_1:31226361-31226380 Msa0543850:CDS 45.0%
CTCAAATGGGTGATCCTCTT+TGG + chr4_1:31226375-31226394 Msa0543850:CDS 45.0%
TATGTTAGGTCGTTTCCTCG+TGG + chr4_1:31226446-31226465 Msa0543850:CDS 45.0%
ATGTTAGGTCGTTTCCTCGT+GGG + chr4_1:31226447-31226466 Msa0543850:CDS 45.0%
GGGATTGGACCTAAACCTAA+TGG + chr4_1:31226467-31226486 Msa0543850:CDS 45.0%
TGTTTCAAGAGCTGTGACAG+TGG + chr4_1:31226816-31226835 Msa0543850:CDS 45.0%
CAAAGTAACTCTCGCGAGTT+GGG - chr4_1:31227230-31227249 None:intergenic 45.0%
ACAAAGTAACTCTCGCGAGT+TGG - chr4_1:31227231-31227250 None:intergenic 45.0%
! CTCGCGAGAGTTACTTTGTA+AGG + chr4_1:31227232-31227251 Msa0543850:CDS 45.0%
AGATGTAGAAGAAGGCCTTC+TGG + chr4_1:31227333-31227352 Msa0543850:CDS 45.0%
CATGTAGAAGTGGATGAGAG+GGG + chr4_1:31227982-31228001 Msa0543850:CDS 45.0%
! GTTAGCACTGGACATGCATT+TGG + chr4_1:31228030-31228049 Msa0543850:CDS 45.0%
AACATTCCCACCGTAGATGT+TGG + chr4_1:31228117-31228136 Msa0543850:CDS 45.0%
ACATACTCTTCCTCCCTTCT+TGG - chr4_1:31228329-31228348 None:intergenic 45.0%
GTTGAAACTGGCAGAGAGAA+TGG + chr4_1:31228347-31228366 Msa0543850:CDS 45.0%
AAAATACCAGGAGGTCTTCC+AGG - chr4_1:31228483-31228502 None:intergenic 45.0%
TTGCAATGTGCGCCATTTCT+TGG - chr4_1:31228715-31228734 None:intergenic 45.0%
GAAATGGCGCACATTGCAAA+TGG + chr4_1:31228716-31228735 Msa0543850:CDS 45.0%
! ATGTTGCTCTGGCTCGAATT+TGG - chr4_1:31229032-31229051 None:intergenic 45.0%
! GAGAGTGTAACAGATGCAAC+AGG + chr4_1:31229065-31229084 Msa0543850:CDS 45.0%
CATTTCCATTTCCATCAGGG+AGG - chr4_1:31229193-31229212 None:intergenic 45.0%
CTCCCTGATGGAAATGGAAA+TGG + chr4_1:31229191-31229210 Msa0543850:CDS 45.0%
GGAAATGGAAATGGAGTCTG+CGG + chr4_1:31229200-31229219 Msa0543850:CDS 45.0%
TTGAAAAGCGACGGTCAGAA+GGG - chr4_1:31229264-31229283 None:intergenic 45.0%
TTTGAAAAGCGACGGTCAGA+AGG - chr4_1:31229265-31229284 None:intergenic 45.0%
!!! GACCGTCGCTTTTCAAATCT+TGG + chr4_1:31229268-31229287 Msa0543850:CDS 45.0%
CCAAGAGAGAGTTTAGCAGT+TGG - chr4_1:31229305-31229324 None:intergenic 45.0%
CCAACTGCTAAACTCTCTCT+TGG + chr4_1:31229302-31229321 Msa0543850:CDS 45.0%
GCTCAACTAAGCACTTTCAC+CGG - chr4_1:31229390-31229409 None:intergenic 45.0%
GCACTAAATGCGGACAAATG+TGG + chr4_1:31229638-31229657 Msa0543850:intron 45.0%
TGTGGCTGCAATCGTGAATT+CGG + chr4_1:31229656-31229675 Msa0543850:intron 45.0%
ATTGAACAAGCGCAACTTCG+AGG - chr4_1:31230010-31230029 None:intergenic 45.0%
AGAGGGACCAGTATTATGTG+AGG + chr4_1:31230468-31230487 Msa0543850:three_prime_UTR 45.0%
! AACCAATTTCTGGACGCGGT+GGG - chr4_1:31226211-31226230 None:intergenic 50.0%
! AAACCAATTTCTGGACGCGG+TGG - chr4_1:31226212-31226231 None:intergenic 50.0%
!! CCGCGTCCAGAAATTGGTTT+TGG + chr4_1:31226212-31226231 Msa0543850:CDS 50.0%
TTTGGCAACCCACAAGGGAT+TGG + chr4_1:31226230-31226249 Msa0543850:CDS 50.0%
! CCCACAAGGGATTGGTACTA+TGG + chr4_1:31226238-31226257 Msa0543850:CDS 50.0%
ATGGACATGTATGGAGCCAG+TGG + chr4_1:31226257-31226276 Msa0543850:CDS 50.0%
GAGCCAGTGGCGATATTCTA+CGG + chr4_1:31226270-31226289 Msa0543850:CDS 50.0%
TGACCGTAGAATATCGCCAC+TGG - chr4_1:31226276-31226295 None:intergenic 50.0%
CAGCAAACACCGTTGACCAT+TGG - chr4_1:31226792-31226811 None:intergenic 50.0%
CAATGGTCAACGGTGTTTGC+TGG + chr4_1:31226790-31226809 Msa0543850:CDS 50.0%
TTCAAGAGCTGTGACAGTGG+AGG + chr4_1:31226819-31226838 Msa0543850:CDS 50.0%
!! ACAGTGGAGGTGTTGTCAAC+TGG + chr4_1:31226832-31226851 Msa0543850:CDS 50.0%
TGTTGTCAACTGGAGTGGCA+GGG + chr4_1:31226842-31226861 Msa0543850:CDS 50.0%
AACAACATCCTGACGGGACA+TGG + chr4_1:31227262-31227281 Msa0543850:CDS 50.0%
ACAACATCCTGACGGGACAT+GGG + chr4_1:31227263-31227282 Msa0543850:CDS 50.0%
GGCAGTTGTGGATGTGTCTT+TGG + chr4_1:31227284-31227303 Msa0543850:CDS 50.0%
CACGACACAGAACCAAACGA+AGG - chr4_1:31227480-31227499 None:intergenic 50.0%
! GGTGGGAATGTTTGTTGCCA+TGG - chr4_1:31228109-31228128 None:intergenic 50.0%
ATTCCCACCGTAGATGTTGG+CGG + chr4_1:31228120-31228139 Msa0543850:CDS 50.0%
ATACCGCCAACATCTACGGT+GGG - chr4_1:31228126-31228145 None:intergenic 50.0%
TATACCGCCAACATCTACGG+TGG - chr4_1:31228127-31228146 None:intergenic 50.0%
GTGCTTCAACAGCACACACA+TGG + chr4_1:31228394-31228413 Msa0543850:CDS 50.0%
CACACATGGACAACACTGTC+TGG + chr4_1:31228408-31228427 Msa0543850:CDS 50.0%
! TGGACAACACTGTCTGGAAC+AGG + chr4_1:31228414-31228433 Msa0543850:CDS 50.0%
!! CGTAAGAGTGTCGATGATCC+TGG + chr4_1:31228462-31228481 Msa0543850:CDS 50.0%
! AAAACTTGTGCTGGCGGAAC+AGG - chr4_1:31228534-31228553 None:intergenic 50.0%
TAACGAGAGCTCCAGAACTG+AGG + chr4_1:31228566-31228585 Msa0543850:CDS 50.0%
CGGTGGAGTTGTCCAAGAAA+TGG + chr4_1:31228700-31228719 Msa0543850:CDS 50.0%
GTGGCAAAGTGCAAGAGAGT+CGG + chr4_1:31228837-31228856 Msa0543850:intron 50.0%
TGCGTTCGCACATGTAGTTG+CGG + chr4_1:31228910-31228929 Msa0543850:intron 50.0%
GGGAGGATAGCAAATCCTGA+TGG - chr4_1:31229176-31229195 None:intergenic 50.0%
!! GGATTTGCTATCCTCCCTGA+TGG + chr4_1:31229179-31229198 Msa0543850:CDS 50.0%
GCTATCCTCCCTGATGGAAA+TGG + chr4_1:31229185-31229204 Msa0543850:CDS 50.0%
AATGGAGTCTGCGGTGAAAC+TGG + chr4_1:31229209-31229228 Msa0543850:CDS 50.0%
! TTCGGGAATTGACTTCCCCT+AGG + chr4_1:31230289-31230308 Msa0543850:three_prime_UTR 50.0%
GAAGTACGTAGGGAAGCCAG+TGG + chr4_1:31226157-31226176 Msa0543850:CDS 55.0%
AGCACAGTAGCAGCTCCATC+AGG - chr4_1:31226413-31226432 None:intergenic 55.0%
AGGTCGTTTCCTCGTGGGAT+TGG + chr4_1:31226452-31226471 Msa0543850:CDS 55.0%
GGTTTAGGTCCAATCCCACG+AGG - chr4_1:31226464-31226483 None:intergenic 55.0%
!! GGAGGTGTTGTCAACTGGAG+TGG + chr4_1:31226837-31226856 Msa0543850:CDS 55.0%
GTGTTGTCAACTGGAGTGGC+AGG + chr4_1:31226841-31226860 Msa0543850:CDS 55.0%
GGAGTGGCAGGGAACTACAA+TGG + chr4_1:31226853-31226872 Msa0543850:CDS 55.0%
! CGAGTTGGGACAAGAGGTGA+AGG - chr4_1:31227216-31227235 None:intergenic 55.0%
!! CGAAAGGCTTGCTAGCTCCA+TGG + chr4_1:31228089-31228108 Msa0543850:CDS 55.0%
GATGATCCTGGAAGACCTCC+TGG + chr4_1:31228474-31228493 Msa0543850:CDS 55.0%
CTCCCACCGCGTCCAGAAAT+TGG + chr4_1:31226206-31226225 Msa0543850:CDS 60.0%
! CTCTCGCGAGTTGGGACAAG+AGG - chr4_1:31227222-31227241 None:intergenic 60.0%
ACAACTGCCCATGTCCCGTC+AGG - chr4_1:31227273-31227292 None:intergenic 60.0%
TGACGGGACATGGGCAGTTG+TGG + chr4_1:31227272-31227291 Msa0543850:CDS 60.0%
GGAGTCTGCGGTGAAACTGG+CGG + chr4_1:31229212-31229231 Msa0543850:CDS 60.0%
TCTGGACGCGGTGGGAGAGA+TGG - chr4_1:31226203-31226222 None:intergenic 65.0%
GTCTGCGGTGAAACTGGCGG+AGG + chr4_1:31229215-31229234 Msa0543850:CDS 65.0%
TGCGGTGAAACTGGCGGAGG+AGG + chr4_1:31229218-31229237 Msa0543850:CDS 65.0%
GGTGAAACTGGCGGAGGAGG+AGG + chr4_1:31229221-31229240 Msa0543850:CDS 65.0%
ACTGGCGGAGGAGGAGGAGC+TGG + chr4_1:31229227-31229246 Msa0543850:CDS 70.0%
GGAGGAGGAGGAGCTGGTGC+TGG + chr4_1:31229233-31229252 Msa0543850:CDS 70.0%
! GGAGGAGGAGCTGGTGCTGG+TGG + chr4_1:31229236-31229255 Msa0543850:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 31226149 31230619 31226149 ID=Msa0543850;Name=Msa0543850
chr4_1 mRNA 31226149 31230619 31226149 ID=Msa0543850-mRNA-1;Parent=Msa0543850;Name=Msa0543850-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|1|0.87|1|0.85|0.87|8|503|547
chr4_1 exon 31226149 31226568 31226149 ID=Msa0543850-mRNA-1:exon:8089;Parent=Msa0543850-mRNA-1
chr4_1 exon 31226787 31226888 31226787 ID=Msa0543850-mRNA-1:exon:8090;Parent=Msa0543850-mRNA-1
chr4_1 exon 31227192 31227392 31227192 ID=Msa0543850-mRNA-1:exon:8091;Parent=Msa0543850-mRNA-1
chr4_1 exon 31227967 31228141 31227967 ID=Msa0543850-mRNA-1:exon:8092;Parent=Msa0543850-mRNA-1
chr4_1 exon 31228316 31228587 31228316 ID=Msa0543850-mRNA-1:exon:8093;Parent=Msa0543850-mRNA-1
chr4_1 exon 31228683 31228778 31228683 ID=Msa0543850-mRNA-1:exon:8094;Parent=Msa0543850-mRNA-1
chr4_1 exon 31229026 31229434 31229026 ID=Msa0543850-mRNA-1:exon:8095;Parent=Msa0543850-mRNA-1
chr4_1 exon 31230148 31230619 31230148 ID=Msa0543850-mRNA-1:exon:8096;Parent=Msa0543850-mRNA-1
chr4_1 CDS 31226149 31226568 31226149 ID=Msa0543850-mRNA-1:cds;Parent=Msa0543850-mRNA-1
chr4_1 CDS 31226787 31226888 31226787 ID=Msa0543850-mRNA-1:cds;Parent=Msa0543850-mRNA-1
chr4_1 CDS 31227192 31227392 31227192 ID=Msa0543850-mRNA-1:cds;Parent=Msa0543850-mRNA-1
chr4_1 CDS 31227967 31228141 31227967 ID=Msa0543850-mRNA-1:cds;Parent=Msa0543850-mRNA-1
chr4_1 CDS 31228316 31228587 31228316 ID=Msa0543850-mRNA-1:cds;Parent=Msa0543850-mRNA-1
chr4_1 CDS 31228683 31228778 31228683 ID=Msa0543850-mRNA-1:cds;Parent=Msa0543850-mRNA-1
chr4_1 CDS 31229026 31229403 31229026 ID=Msa0543850-mRNA-1:cds;Parent=Msa0543850-mRNA-1
chr4_1 three_prime_UTR 31229404 31229434 31229404 ID=Msa0543850-mRNA-1:three_prime_utr;Parent=Msa0543850-mRNA-1
chr4_1 three_prime_UTR 31230148 31230619 31230148 ID=Msa0543850-mRNA-1:three_prime_utr;Parent=Msa0543850-mRNA-1
Gene Sequence

>Msa0543850

ATGGCAGCGAAGTACGTAGGGAAGCCAGTGGTAAACTACTCAAACATTTCACCATCTCTCCCACCGCGTCCAGAAATTGGTTTTGGCAACCCACAAGGGATTGGTACTATGGACATGTATGGAGCCAGTGGCGATATTCTACGGTCAATTAGTGGGCCGACCGAAGCAGATAAACCGATAATAATAGAGTTAGCTGTTGCAGCTATGGAAGAGCTTATTGGGATGGCTCAAATGGGTGATCCTCTTTGGTTAAGTACTACACCTGATGGAGCTGCTACTGTGCTTAATGAAGATGAATATGTTAGGTCGTTTCCTCGTGGGATTGGACCTAAACCTAATGGTTTTAAATGTGAAGCTTCGAGGGAATCTAGCGTTGTTATCATGAATCATGTCAACCTTGTTGAGATTCTCATGGATGTGAACCAATGGTCAACGGTGTTTGCTGGTATTGTTTCAAGAGCTGTGACAGTGGAGGTGTTGTCAACTGGAGTGGCAGGGAACTACAATGGTGCTTTACAAGTGATGACAGCAGAGTTTCAAGTACCTTCACCTCTTGTCCCAACTCGCGAGAGTTACTTTGTAAGGTATTGTAAACAACATCCTGACGGGACATGGGCAGTTGTGGATGTGTCTTTGGATAATCTGCGCCCTAGTCCTTCATCTAGATGTAGAAGAAGGCCTTCTGGTTGCTTAATTCAAGAGATGCCAAATGGTTACTCAAAGGTGACATGGGTTGAACATGTAGAAGTGGATGAGAGGGGTGTTCATAATCTATACAAGCAGATTGTTAGCACTGGACATGCATTTGGTGCAAAACGTTGGGTTGCAACTTTAGATCGACAATGCGAAAGGCTTGCTAGCTCCATGGCAACAAACATTCCCACCGTAGATGTTGGCGTGATAACAAACCAAGAAGGGAGGAAGAGTATGTTGAAACTGGCAGAGAGAATGGTGATAAGTTTTTGTGGTGGAGTGAGTGCTTCAACAGCACACACATGGACAACACTGTCTGGAACAGGAGCCGATGATGTGAGAGTGATGACTCGTAAGAGTGTCGATGATCCTGGAAGACCTCCTGGTATTTTTCTTAGTGCTGCTACTTCTTTTTGGCTTCCTGTTCCGCCAGCACAAGTTTTTGAATTTCTTCGTAACGAGAGCTCCAGAACTGAGTGGGATATTCTTTCTAACGGTGGAGTTGTCCAAGAAATGGCGCACATTGCAAATGGACGTGATACTGGAAACTGTGTCTCTCTCCTTAGAGTAAATAGTCCAAATTCGAGCCAGAGCAACATGTTAATACTACAAGAGAGTGTAACAGATGCAACAGGCTCATTTGTTATCTATGCACCGGTAGATATGGTTGCTATGAATGTAGTTCTAAATGGAGGAGATCCAGATTATGTAGCACTTCTTCCATCAGGATTTGCTATCCTCCCTGATGGAAATGGAAATGGAGTCTGCGGTGAAACTGGCGGAGGAGGAGGAGCTGGTGCTGGTGGATCCCTTCTGACCGTCGCTTTTCAAATCTTGGTTGATTCTACTCCAACTGCTAAACTCTCTCTTGGATCAGTTGCTACTGTTAATAGTCTTATTGCTTGTACTGTCGAGAGAATCAAAGCTTCTTTATCCGGTGAAAGTGCTTAG
Protein sequence

>Msa0543850

MAAKYVGKPVVNYSNISPSLPPRPEIGFGNPQGIGTMDMYGASGDILRSISGPTEADKPIIIELAVAAMEELIGMAQMGDPLWLSTTPDGAATVLNEDEYVRSFPRGIGPKPNGFKCEASRESSVVIMNHVNLVEILMDVNQWSTVFAGIVSRAVTVEVLSTGVAGNYNGALQVMTAEFQVPSPLVPTRESYFVRYCKQHPDGTWAVVDVSLDNLRPSPSSRCRRRPSGCLIQEMPNGYSKVTWVEHVEVDERGVHNLYKQIVSTGHAFGAKRWVATLDRQCERLASSMATNIPTVDVGVITNQEGRKSMLKLAERMVISFCGGVSASTAHTWTTLSGTGADDVRVMTRKSVDDPGRPPGIFLSAATSFWLPVPPAQVFEFLRNESSRTEWDILSNGGVVQEMAHIANGRDTGNCVSLLRVNSPNSSQSNMLILQESVTDATGSFVIYAPVDMVAMNVVLNGGDPDYVALLPSGFAILPDGNGNGVCGETGGGGGAGAGGSLLTVAFQILVDSTPTAKLSLGSVATVNSLIACTVERIKASLSGESA*