AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1295930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1295930 MtrunA17_Chr8g0373541 96.750 523 10 2 1 520 1 519 0.0 1025
Msa1295930 MtrunA17_Chr4g0040571 47.700 413 164 7 29 412 35 424 1.74e-118 358
Msa1295930 MtrunA17_Chr2g0330171 62.687 268 83 2 4 254 1 268 6.86e-118 355
Msa1295930 MtrunA17_Chr8g0342421 76.077 209 35 2 3 196 17 225 6.51e-117 354
Msa1295930 MtrunA17_Chr1g0191991 70.175 228 65 2 31 255 61 288 1.90e-116 353
Msa1295930 MtrunA17_Chr1g0181381 84.091 176 22 2 26 195 26 201 3.51e-111 339
Msa1295930 MtrunA17_Chr1g0162971 47.242 417 150 10 33 425 41 411 3.66e-111 337
Msa1295930 MtrunA17_Chr4g0029521 76.842 190 39 2 11 195 12 201 5.57e-111 338
Msa1295930 MtrunA17_Chr1g0185121 47.666 407 120 10 4 396 6 333 1.49e-110 336
Msa1295930 MtrunA17_Chr7g0263941 79.032 186 34 1 15 195 11 196 1.33e-109 332
Msa1295930 MtrunA17_Chr1g0199101 85.185 162 24 0 31 192 45 206 9.30e-109 334
Msa1295930 MtrunA17_Chr1g0163001 84.242 165 26 0 31 195 41 205 1.31e-107 331
Msa1295930 MtrunA17_Chr1g0151351 85.366 164 23 1 32 194 40 203 3.36e-107 327
Msa1295930 MtrunA17_Chr1g0210821 83.439 157 26 0 36 192 47 203 4.74e-104 323
Msa1295930 MtrunA17_Chr7g0268961 86.928 153 20 0 43 195 32 184 1.25e-103 319
Msa1295930 MtrunA17_Chr3g0141431 80.247 162 31 1 29 189 18 179 4.20e-100 307
Msa1295930 MtrunA17_Chr2g0326381 77.576 165 36 1 32 195 31 195 8.76e-100 307
Msa1295930 MtrunA17_Chr2g0324991 90.076 131 13 0 44 174 3 133 5.26e-88 267
Msa1295930 MtrunA17_Chr6g0479921 63.253 166 60 1 33 197 29 194 5.69e-75 244
Msa1295930 MtrunA17_Chr8g0375521 57.143 189 67 4 4 186 2 182 1.26e-72 237
Msa1295930 MtrunA17_Chr3g0123941 32.642 193 118 4 38 218 113 305 3.18e-24 103
Msa1295930 MtrunA17_Chr2g0320341 34.839 155 87 3 42 182 224 378 2.26e-23 102
Msa1295930 MtrunA17_Chr1g0200421 34.437 151 87 3 47 185 236 386 4.03e-23 102
Msa1295930 MtrunA17_Chr5g0422571 32.243 214 130 7 9 212 65 273 1.96e-22 97.8
Msa1295930 MtrunA17_Chr8g0382121 35.762 151 86 5 43 182 106 256 3.49e-21 94.4
Msa1295930 MtrunA17_Chr7g0258011 33.548 155 85 5 56 193 183 336 4.38e-19 88.6
Msa1295930 MtrunA17_Chr8g0352011 35.256 156 81 6 56 193 173 326 9.76e-19 87.4
Msa1295930 MtrunA17_Chr4g0053341 33.113 151 91 4 44 185 69 218 4.26e-18 83.6
Msa1295930 MtrunA17_Chr1g0198651 32.680 153 90 4 56 196 109 260 7.54e-18 84.3
Msa1295930 MtrunA17_Chr1g0173841 34.532 139 81 4 56 185 222 359 8.54e-18 85.5
Msa1295930 MtrunA17_Chr4g0026521 35.252 139 80 5 56 185 125 262 1.25e-17 83.6
Msa1295930 MtrunA17_Chr2g0328921 32.927 164 92 6 31 185 128 282 3.15e-17 82.8
Msa1295930 MtrunA17_Chr2g0321291 33.333 168 98 6 30 185 112 277 4.48e-17 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1295930 AT5G66730.1 58.041 541 162 16 2 520 3 500 5.77e-180 515
Msa1295930 AT3G50700.1 56.346 520 151 14 2 518 3 449 5.48e-179 511
Msa1295930 AT1G03840.1 86.228 167 23 0 29 195 37 203 3.64e-111 340
Msa1295930 AT5G44160.1 78.351 194 40 1 4 195 6 199 7.71e-111 337
Msa1295930 AT1G55110.3 70.507 217 49 5 3 204 20 236 4.30e-108 330
Msa1295930 AT1G55110.1 70.507 217 49 5 3 204 20 236 4.30e-108 330
Msa1295930 AT1G55110.2 70.507 217 49 5 3 204 20 236 4.30e-108 330
Msa1295930 AT1G03840.2 85.030 167 23 1 29 195 37 201 8.06e-108 331
Msa1295930 AT3G13810.5 70.233 215 42 2 3 195 14 228 2.80e-106 327
Msa1295930 AT3G13810.4 70.233 215 42 2 3 195 19 233 3.04e-106 327
Msa1295930 AT3G13810.1 70.233 215 42 2 3 195 19 233 3.04e-106 327
Msa1295930 AT3G13810.6 70.561 214 41 2 4 195 1 214 3.57e-106 326
Msa1295930 AT2G02080.1 78.916 166 35 0 31 196 52 217 6.39e-106 327
Msa1295930 AT2G02080.4 78.916 166 35 0 31 196 52 217 6.39e-106 327
Msa1295930 AT2G02080.5 78.916 166 35 0 31 196 52 217 6.39e-106 327
Msa1295930 AT2G02080.3 78.916 166 35 0 31 196 52 217 6.39e-106 327
Msa1295930 AT4G02670.1 61.628 258 78 5 8 244 19 276 1.88e-105 321
Msa1295930 AT4G02670.2 61.628 258 78 5 8 244 21 278 2.19e-105 321
Msa1295930 AT1G14580.1 76.608 171 40 0 26 196 46 216 8.00e-105 322
Msa1295930 AT1G14580.2 76.608 171 40 0 26 196 46 216 8.00e-105 322
Msa1295930 AT5G03150.1 74.872 195 41 3 33 219 53 247 9.65e-105 323
Msa1295930 AT1G14580.3 76.608 171 40 0 26 196 60 230 1.34e-104 322
Msa1295930 AT3G45260.2 81.098 164 30 1 33 195 39 202 5.03e-104 319
Msa1295930 AT3G45260.1 81.098 164 30 1 33 195 39 202 5.03e-104 319
Msa1295930 AT5G44160.2 88.235 153 18 0 43 195 8 160 1.07e-102 315
Msa1295930 AT2G02070.2 80.368 163 32 0 33 195 52 214 1.17e-102 321
Msa1295930 AT2G02070.1 80.368 163 32 0 33 195 52 214 1.17e-102 321
Msa1295930 AT5G60470.2 77.246 167 37 1 30 195 41 207 3.58e-100 310
Msa1295930 AT5G60470.3 77.246 167 37 1 30 195 37 203 4.11e-100 310
Msa1295930 AT3G13810.3 87.582 153 18 1 44 195 68 220 9.75e-99 307
Msa1295930 AT3G13810.2 87.582 153 18 1 44 195 82 234 1.38e-98 307
Msa1295930 AT5G60470.1 70.520 173 50 1 24 195 26 198 4.05e-94 294
Msa1295930 AT2G02080.6 80.714 140 27 0 57 196 1 140 1.66e-91 286
Msa1295930 AT2G02080.2 80.714 140 27 0 57 196 1 140 1.66e-91 286
Msa1295930 AT1G25250.3 68.153 157 47 2 33 186 33 189 3.95e-75 243
Msa1295930 AT1G68130.1 67.308 156 49 2 33 186 41 196 3.07e-74 241
Msa1295930 AT2G01940.1 65.190 158 53 2 33 188 44 201 2.02e-73 240
Msa1295930 AT1G25250.1 68.456 149 44 2 41 186 18 166 9.62e-72 233
Msa1295930 AT2G01940.3 63.522 159 55 3 33 188 44 202 3.48e-69 229
Msa1295930 AT1G25250.2 67.568 111 33 2 79 186 1 111 1.34e-47 167
Msa1295930 AT1G68130.2 65.455 110 36 2 79 186 1 110 2.01e-46 165
Msa1295930 AT2G01940.2 64.286 112 38 2 79 188 1 112 2.66e-46 166
Msa1295930 AT5G22890.1 34.000 150 85 3 47 182 202 351 4.10e-23 101
Msa1295930 AT1G34370.3 33.962 159 91 3 41 185 13 171 5.17e-23 99.8
Msa1295930 AT1G34370.2 33.962 159 91 3 41 185 223 381 1.09e-22 101
Msa1295930 AT1G34370.1 33.962 159 91 3 41 185 223 381 1.09e-22 101
Msa1295930 AT1G13290.1 36.986 146 80 6 56 190 95 239 3.97e-19 88.6
Msa1295930 AT1G34790.1 34.667 150 88 4 45 185 128 276 2.21e-17 83.6
Msa1295930 AT1G51220.1 33.094 139 83 4 56 185 172 309 3.50e-17 83.2
Msa1295930 AT3G57670.1 30.588 170 100 5 56 208 211 379 3.62e-17 84.0
Msa1295930 AT3G20880.1 33.813 139 82 4 56 185 250 387 6.93e-17 83.2
Msa1295930 AT1G08290.1 36.296 135 75 4 61 185 179 312 8.58e-17 82.0

Find 156 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCCTTGTGATGGCGATTC+TGG 0.160435 8_3:-54418796 Msa1295930:CDS
ATATGGTTGTCTTAAGATTT+AGG 0.166620 8_3:-54418237 Msa1295930:three_prime_UTR
CAGAAGAAAGACTATATTTA+AGG 0.201460 8_3:-54418338 Msa1295930:three_prime_UTR
TTGTGGTTCCTCTATGATTT+GGG 0.227141 8_3:+54419350 None:intergenic
ACACAGGCTGTGAGAAATTA+AGG 0.244185 8_3:-54418268 Msa1295930:three_prime_UTR
CATGATCCTTCTAGAGCTTT+AGG 0.245902 8_3:-54420482 Msa1295930:CDS
GAAGTTCATGATAGATATTT+TGG 0.250600 8_3:-54418564 Msa1295930:exon
GTCTTTCTTCTGGCATTATA+AGG 0.270969 8_3:+54418349 None:intergenic
CTTGTGGTTCCTCTATGATT+TGG 0.282265 8_3:+54419349 None:intergenic
CAACTACTAGTGTTATTATC+AGG 0.290858 8_3:-54418532 Msa1295930:three_prime_UTR
CGTAGCTGTAGGAATAAATT+AGG 0.294046 8_3:-54418124 Msa1295930:three_prime_UTR
AGAGCTTTAGGTGATTTAAC+TGG 0.295896 8_3:-54420470 Msa1295930:CDS
AAAGATCAAGTAAAGAAATT+AGG 0.296295 8_3:-54420544 Msa1295930:CDS
GACAATGTTTCCACAGCTTC+AGG 0.299307 8_3:-54420892 Msa1295930:CDS
GGACTAAAGGAATTGATGTT+AGG 0.301999 8_3:-54418775 Msa1295930:CDS
TATGGTTGTCTTAAGATTTA+GGG 0.302414 8_3:-54418236 Msa1295930:three_prime_UTR
AGATTGAACAGCATATTTCT+TGG 0.304180 8_3:+54420388 None:intergenic
CTTAAATATAGTCTTTCTTC+TGG 0.312522 8_3:+54418339 None:intergenic
CTAGGATTGGGGATGGCTGC+TGG 0.321231 8_3:-54418709 Msa1295930:CDS
ACTCTTGATTTCCTAGGATT+GGG 0.333570 8_3:-54418721 Msa1295930:CDS
TATTCCTCAGATGGATGATT+TGG 0.337901 8_3:-54418149 Msa1295930:three_prime_UTR
GCTTCTCTGCTTCGTGGGCT+TGG 0.339781 8_3:-54418934 Msa1295930:CDS
AAGGTACAATGTGCTTGTTT+TGG 0.357051 8_3:-54418294 Msa1295930:three_prime_UTR
AACTCTTGATTTCCTAGGAT+TGG 0.365068 8_3:-54418722 Msa1295930:CDS
CAATATGCAATATGCAACTA+TGG 0.365464 8_3:-54418409 Msa1295930:three_prime_UTR
CGGCGTTCAGCGAACTAATT+AGG 0.366521 8_3:-54419170 Msa1295930:CDS
AGAGCAATCACCTCTGCTTC+TGG 0.372321 8_3:+54420689 None:intergenic
CATAGTTGCATATTGCATAT+TGG 0.373692 8_3:+54418410 None:intergenic
GAGTTCTCTGGCAAAGATAT+TGG 0.378609 8_3:-54418589 Msa1295930:CDS
TGCAACAGGTAATGTGCAAA+TGG 0.385687 8_3:-54418381 Msa1295930:three_prime_UTR
CAAGTAAAGAAATTAGGAAA+AGG 0.395504 8_3:-54420538 Msa1295930:CDS
TTCCACGTTTGTCCATTGTT+TGG 0.395965 8_3:-54418046 Msa1295930:three_prime_UTR
ATGGCTGCTGGTGGTAGTGC+CGG 0.397095 8_3:-54418697 Msa1295930:CDS
ATAGCTATAGTTTATAGCTT+AGG 0.399501 8_3:-54420958 Msa1295930:five_prime_UTR
TCTTCTGCTTCCACTTCATC+TGG 0.404493 8_3:-54418901 Msa1295930:CDS
AGTTTAGTTTCAGCAGGACT+TGG 0.406784 8_3:-54418826 Msa1295930:CDS
CAGACAACTCTTGATTTCCT+AGG 0.415465 8_3:-54418727 Msa1295930:CDS
GATAGCCTGCATTGGGGTCT+CGG 0.418830 8_3:-54418871 Msa1295930:CDS
TTAGGGACTCCGTCAATGTT+TGG 0.420641 8_3:-54418757 Msa1295930:CDS
TGGAATTATTGTCACATTTG+TGG 0.424814 8_3:-54418071 Msa1295930:three_prime_UTR
AATATGCTGTTCAATCTGAT+TGG 0.427517 8_3:-54420382 Msa1295930:CDS
GAAAATTGATGATCCATGAT+TGG 0.434295 8_3:+54419068 None:intergenic
ACCGCAGCAGCCGCATCCTT+CGG 0.436819 8_3:-54418619 Msa1295930:CDS
TTTGTAGCTAACAACGTTGT+TGG 0.437085 8_3:+54420662 None:intergenic
AAGCACATTGTACCTTGTTA+AGG 0.439079 8_3:+54418301 None:intergenic
AAAGCTCACTCTAAAGTTTG+TGG 0.439997 8_3:-54420359 Msa1295930:CDS
AAGTAAAGAAATTAGGAAAA+GGG 0.440652 8_3:-54420537 Msa1295930:CDS
GCAACAGGTAATGTGCAAAT+GGG 0.445106 8_3:-54418380 Msa1295930:three_prime_UTR
AATTGGCGGTGGCAGTGGTC+TGG 0.449758 8_3:-54418647 Msa1295930:CDS
TCTGCACTTATCACGTCAAT+TGG 0.451085 8_3:-54418664 Msa1295930:CDS
CTTTGCCTCTCGACCAATCA+TGG 0.452274 8_3:-54419081 Msa1295930:CDS
GCATTGGGGTCTCGGACAAA+TGG 0.454354 8_3:-54418863 Msa1295930:CDS
TCAACAAGATAGCCTGCATT+GGG 0.455422 8_3:-54418878 Msa1295930:CDS
GGTTCTAGTTTAGTTTCAGC+AGG 0.456585 8_3:-54418832 Msa1295930:CDS
TGATGAACACAAGTTGGTTC+AGG 0.461372 8_3:+54420503 None:intergenic
CAACTATGGATCAATGCAAC+AGG 0.464041 8_3:-54418395 Msa1295930:three_prime_UTR
ACGTCAATTGGCGGTGGCAG+TGG 0.464341 8_3:-54418652 Msa1295930:CDS
GATCTTTGTCTCAGTTTCCA+TGG 0.464582 8_3:+54420560 None:intergenic
TAGTGGTGTGTCCTCTGCTT+TGG 0.468292 8_3:-54419989 Msa1295930:CDS
AAATCACCTAAAGCTCTAGA+AGG 0.470379 8_3:+54420476 None:intergenic
GTTTCAGCAGGACTTGGACT+AGG 0.476162 8_3:-54418820 Msa1295930:CDS
GCAGCCGCATCCTTCGGTAA+TGG 0.482125 8_3:-54418613 Msa1295930:CDS
AAATCAAGAGTTGTCTGCTT+AGG 0.482911 8_3:+54418733 None:intergenic
CAAACGCTTCTCTGCTTCGT+GGG 0.483200 8_3:-54418939 Msa1295930:CDS
GACTAAAGGAATTGATGTTA+GGG 0.488496 8_3:-54418774 Msa1295930:CDS
CAGCGAACTAATTAGGTCCA+TGG 0.494426 8_3:-54419163 Msa1295930:CDS
TTCGGTAATGGAGAGTTCTC+TGG 0.494928 8_3:-54418601 Msa1295930:CDS
AGAAATCTCCCAGGAATGCC+AGG 0.500143 8_3:-54420799 Msa1295930:intron
TTCTGCGAGTCTCCAACCTC+AGG 0.501471 8_3:-54419196 Msa1295930:CDS
ACAAACGCTTCTCTGCTTCG+TGG 0.502867 8_3:-54418940 Msa1295930:CDS
CAAATCATGTATTCCTCAGA+TGG 0.506633 8_3:-54418158 Msa1295930:three_prime_UTR
GGCTGTGCTACTACCAGCAC+TGG 0.506941 8_3:-54419258 Msa1295930:CDS
CAGCAGCCATCCCCAATCCT+AGG 0.507214 8_3:+54418710 None:intergenic
GAAAGACTATATTTAAGGAA+AGG 0.510662 8_3:-54418333 Msa1295930:three_prime_UTR
TCGCTGAACGCCGGTGCCTG+AGG 0.511411 8_3:+54419180 None:intergenic
GCTGATGTGTAGGCACGTAT+TGG 0.511427 8_3:+54419022 None:intergenic
AAATTAAGGGTATGTGCATA+TGG 0.514034 8_3:-54418254 Msa1295930:three_prime_UTR
GTCAACAAGATAGCCTGCAT+TGG 0.514883 8_3:-54418879 Msa1295930:CDS
CATTGTCTAAATCAACCATT+GGG 0.515965 8_3:+54420908 None:intergenic
CACAGGCTGTGAGAAATTAA+GGG 0.516414 8_3:-54418267 Msa1295930:three_prime_UTR
ACATTGTCTAAATCAACCAT+TGG 0.516475 8_3:+54420907 None:intergenic
TGAACGCCGGTGCCTGAGGT+TGG 0.520285 8_3:+54419184 None:intergenic
TAATTCAGAATCTGATTCAA+AGG 0.520820 8_3:-54420088 Msa1295930:CDS
AATGCAGTGGCAGACATCGC+AGG 0.522789 8_3:+54418997 None:intergenic
GCTCGCAAACACGCCAGTGC+TGG 0.524696 8_3:+54419245 None:intergenic
GAAGCTAGCATCTCTTCCTC+TGG 0.524982 8_3:-54420868 Msa1295930:CDS
TGATTTCCTAGGATTGGGGA+TGG 0.525019 8_3:-54418716 Msa1295930:CDS
AGAGAGATCGCCTCGGACGA+TGG 0.526923 8_3:+54419102 None:intergenic
ACTACTCCTCAGTCAAATAG+TGG 0.528176 8_3:-54420006 Msa1295930:CDS
CTAATTTATTCCTACAGCTA+CGG 0.530535 8_3:+54418125 None:intergenic
CTCTTGATTTCCTAGGATTG+GGG 0.537741 8_3:-54418720 Msa1295930:CDS
TGATGGCGATTCTGGACTAA+AGG 0.543845 8_3:-54418788 Msa1295930:CDS
AGTGGAGCATTTGAAGATCA+TGG 0.543917 8_3:-54418211 Msa1295930:three_prime_UTR
GCACTTATCACGTCAATTGG+CGG 0.543972 8_3:-54418661 Msa1295930:CDS
TGTGTGAAATATGTAACAAA+GGG 0.546390 8_3:-54420631 Msa1295930:CDS
GAGCTTTAGGTGATTTAACT+GGG 0.546954 8_3:-54420469 Msa1295930:CDS
TTTGACCGGTGATTCGAAGC+CGG 0.547642 8_3:-54420064 Msa1295930:CDS
CGACACCGGCTTCGAATCAC+CGG 0.547911 8_3:+54420059 None:intergenic
TGCTTAGGACCAAACATTGA+CGG 0.548417 8_3:+54418748 None:intergenic
GCTGGTGGTAGTGCCGGTGG+AGG 0.548542 8_3:-54418691 Msa1295930:CDS
GGACTAGGTCTTCCTTGTGA+TGG 0.549198 8_3:-54418805 Msa1295930:CDS
GCTGCTGGTGGTAGTGCCGG+TGG 0.551709 8_3:-54418694 Msa1295930:CDS
GTGTGTGAAATATGTAACAA+AGG 0.553618 8_3:-54420632 Msa1295930:CDS
ACCGAAGGATGCGGCTGCTG+CGG 0.555962 8_3:+54418618 None:intergenic
GTCTTAAGATTTAGGGACAG+TGG 0.559908 8_3:-54418229 Msa1295930:three_prime_UTR
TTTGTCCGAGACCCCAATGC+AGG 0.560302 8_3:+54418866 None:intergenic
GGATCATGATGAACACAAGT+TGG 0.562485 8_3:+54420497 None:intergenic
AGAGAGTATAAATGTGATTG+TGG 0.563676 8_3:-54420332 Msa1295930:CDS
AACTACTAGTGTTATTATCA+GGG 0.565154 8_3:-54418531 Msa1295930:three_prime_UTR
TGTGGTTCCTCTATGATTTG+GGG 0.567711 8_3:+54419351 None:intergenic
CTCTCCATTACCGAAGGATG+CGG 0.569694 8_3:+54418609 None:intergenic
GAAACTAAACTAGAACCCTC+TGG 0.570954 8_3:+54418838 None:intergenic
AGCGAACTAATTAGGTCCAT+GGG 0.573274 8_3:-54419162 Msa1295930:CDS
GCTAGCTTCACCTGAAGCTG+TGG 0.576380 8_3:+54420882 None:intergenic
GGTTCCTCTATGATTTGGGG+TGG 0.577325 8_3:+54419354 None:intergenic
ATCTTGTTGACCAGATGAAG+TGG 0.580608 8_3:+54418891 None:intergenic
TTGAATATTGTTATTACCAG+AGG 0.581923 8_3:+54420852 None:intergenic
TTTGAAGTTGCAATTGACAT+GGG 0.586590 8_3:-54418459 Msa1295930:three_prime_UTR
AATCTTCAACTACATAGAAG+AGG 0.593928 8_3:-54420593 Msa1295930:CDS
ATCACCGCTGGCATCGACAC+CGG 0.594080 8_3:+54420045 None:intergenic
TCATATCAAATATTTCCCAA+TGG 0.594960 8_3:-54420923 Msa1295930:exon
CTTATCACGTCAATTGGCGG+TGG 0.597820 8_3:-54418658 Msa1295930:CDS
CTCAGCTCCTCCATCGTCCG+AGG 0.601921 8_3:-54419112 Msa1295930:CDS
GGATTGGGGATGGCTGCTGG+TGG 0.602431 8_3:-54418706 Msa1295930:CDS
GGAGGAGCTGAGAAATCTGA+AGG 0.603033 8_3:+54419123 None:intergenic
AAGTGCAGACAAGCCTCCAC+CGG 0.604756 8_3:+54418678 None:intergenic
AAATCCACCCCAAATCATAG+AGG 0.606558 8_3:-54419358 Msa1295930:CDS
TAATAATCTTTGTACAAACA+AGG 0.606778 8_3:+54420991 None:intergenic
GCTTTGGAGACTCAGAAGCT+AGG 0.609936 8_3:-54419973 Msa1295930:intron
ATGATCCATGATTGGTCGAG+AGG 0.611193 8_3:+54419076 None:intergenic
AGGAAAATGTGACCTTAACA+AGG 0.612233 8_3:-54418313 Msa1295930:three_prime_UTR
ACGAGGATCTGTACATCCCA+TGG 0.612346 8_3:+54419146 None:intergenic
ATGATTTGGTCCGTAGCTGT+AGG 0.616680 8_3:-54418135 Msa1295930:three_prime_UTR
CAACAGGTAATGTGCAAATG+GGG 0.617469 8_3:-54418379 Msa1295930:three_prime_UTR
ACGCATTAAGTGCCAAACAA+TGG 0.618876 8_3:+54418034 None:intergenic
ATCGCAGGAGGCTGATGTGT+AGG 0.621043 8_3:+54419012 None:intergenic
GAAGAGGACATAATCTACCA+TGG 0.626841 8_3:-54420577 Msa1295930:CDS
GCAGTAGTAGTAATCACCGC+TGG 0.627889 8_3:+54420033 None:intergenic
GAGTCTCCAACCTCAGGCAC+CGG 0.629519 8_3:-54419190 Msa1295930:CDS
AGTACTAGCTCAACAAGCAA+CGG 0.630248 8_3:-54419279 Msa1295930:CDS
CTAATTAGTTCGCTGAACGC+CGG 0.631396 8_3:+54419171 None:intergenic
GCTGAGAAATCTGAAGGACG+AGG 0.632449 8_3:+54419129 None:intergenic
GAGATCGCCTCGGACGATGG+AGG 0.640921 8_3:+54419105 None:intergenic
TCTCGGACAAATGGAACCAG+AGG 0.640999 8_3:-54418854 Msa1295930:CDS
AGTCCAGAATCGCCATCACA+AGG 0.641532 8_3:+54418793 None:intergenic
AGAGAACTCTCCATTACCGA+AGG 0.648524 8_3:+54418603 None:intergenic
CGGACCAAATCATCCATCTG+AGG 0.650001 8_3:+54418145 None:intergenic
GAAGCCGGTGTCGATGCCAG+CGG 0.666236 8_3:-54420049 Msa1295930:CDS
GAGGCAAAGAGAGATCGCCT+CGG 0.672821 8_3:+54419095 None:intergenic
CTCGGACAAATGGAACCAGA+GGG 0.680399 8_3:-54418853 Msa1295930:CDS
CTTCTGAGTCTCCAAAGCAG+AGG 0.681133 8_3:+54419978 None:intergenic
GCAGTGGCAGACATCGCAGG+AGG 0.685993 8_3:+54419000 None:intergenic
CAACAAGATAGCCTGCATTG+GGG 0.703664 8_3:-54418877 Msa1295930:CDS
TGCCAAACAATGGACAAACG+TGG 0.705749 8_3:+54418044 None:intergenic
GACACACCACTATTTGACTG+AGG 0.723609 8_3:+54420000 None:intergenic
GTAGTAGTAACAACAACTTG+TGG 0.766984 8_3:+54419333 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTAAATTTTTATTTT+TGG - chr8_3:54418824-54418843 Msa1295930:CDS 0.0%
!!! TTTTATATGTTTATGAAAAA+TGG - chr8_3:54418862-54418881 Msa1295930:CDS 10.0%
!! TTTATATGTTTATGAAAAAT+GGG - chr8_3:54418863-54418882 Msa1295930:CDS 10.0%
!!! TTTTTTTTTTTTGTTTGCTA+GGG + chr8_3:54418048-54418067 None:intergenic 15.0%
!!! TTTTTTTTTTTTTGTTTGCT+AGG + chr8_3:54418049-54418068 None:intergenic 15.0%
!!! TATTTATTAATTTTTGTACC+TGG + chr8_3:54418334-54418353 None:intergenic 15.0%
!! AAATTTAATAAAAAAGAACC+TGG + chr8_3:54418817-54418836 None:intergenic 15.0%
!!! CAAATTCAAAATTTTGTTTT+TGG - chr8_3:54419271-54419290 Msa1295930:CDS 15.0%
!! TTTAATGTAACATAAAAGAA+AGG - chr8_3:54419571-54419590 Msa1295930:intron 15.0%
!! ATTATAATCATCAACTAATA+GGG + chr8_3:54419650-54419669 None:intergenic 15.0%
!! AATTATAATCATCAACTAAT+AGG + chr8_3:54419651-54419670 None:intergenic 15.0%
!!! AAATCAGTAAGAAACTTTTT+TGG + chr8_3:54418080-54418099 None:intergenic 20.0%
!! TAATAATCTTTGTACAAACA+AGG + chr8_3:54418124-54418143 None:intergenic 20.0%
!! AAAGATCAAGTAAAGAAATT+AGG - chr8_3:54418568-54418587 Msa1295930:exon 20.0%
!! AAGTAAAGAAATTAGGAAAA+GGG - chr8_3:54418575-54418594 Msa1295930:exon 20.0%
!!! TTTTATGTGTTTGATTTTGA+AGG - chr8_3:54418915-54418934 Msa1295930:CDS 20.0%
!!! TGTTTAGTATTTTTTTTGTC+TGG - chr8_3:54419321-54419340 Msa1295930:CDS 20.0%
!! TGAAAATAAATCCCTTAAAA+AGG - chr8_3:54419422-54419441 Msa1295930:intron 20.0%
!!! TACAAAAAACACCTTTTTAA+GGG + chr8_3:54419436-54419455 None:intergenic 20.0%
!!! TTACAAAAAACACCTTTTTA+AGG + chr8_3:54419437-54419456 None:intergenic 20.0%
! ATAGCTATAGTTTATAGCTT+AGG - chr8_3:54418154-54418173 Msa1295930:three_prime_UTR 25.0%
! TCATATCAAATATTTCCCAA+TGG - chr8_3:54418189-54418208 Msa1295930:three_prime_UTR 25.0%
!! TTGAATATTGTTATTACCAG+AGG + chr8_3:54418263-54418282 None:intergenic 25.0%
!!! AGATTTCTTTTTTTCTTGGT+GGG + chr8_3:54418301-54418320 None:intergenic 25.0%
! TGTGTGAAATATGTAACAAA+GGG - chr8_3:54418481-54418500 Msa1295930:three_prime_UTR 25.0%
! CAAGTAAAGAAATTAGGAAA+AGG - chr8_3:54418574-54418593 Msa1295930:exon 25.0%
! AAAAAAGAACCTGGAAAAAA+CGG + chr8_3:54418808-54418827 None:intergenic 25.0%
! TAATTCAGAATCTGATTCAA+AGG - chr8_3:54419024-54419043 Msa1295930:CDS 25.0%
!!! AAAGTGCAATTTTTGTTTAC+TGG - chr8_3:54419194-54419213 Msa1295930:CDS 25.0%
!!! AAGTGCAATTTTTGTTTACT+GGG - chr8_3:54419195-54419214 Msa1295930:CDS 25.0%
!!! TTTTTTTGTCATGTTTGATG+TGG - chr8_3:54419231-54419250 Msa1295930:CDS 25.0%
! GTTTGATCTAAATTATGCAT+TGG - chr8_3:54419459-54419478 Msa1295930:intron 25.0%
!!! AGAAGATTTGTTTTTAAGAG+TGG - chr8_3:54419510-54419529 Msa1295930:intron 25.0%
!! AATCATGGATCATCAATTTT+CGG - chr8_3:54420046-54420065 Msa1295930:CDS 25.0%
!! GAAGTTCATGATAGATATTT+TGG - chr8_3:54420548-54420567 Msa1295930:CDS 25.0%
!! AACTACTAGTGTTATTATCA+GGG - chr8_3:54420581-54420600 Msa1295930:CDS 25.0%
! CTTAAATATAGTCTTTCTTC+TGG + chr8_3:54420776-54420795 None:intergenic 25.0%
! CAGAAGAAAGACTATATTTA+AGG - chr8_3:54420774-54420793 Msa1295930:intron 25.0%
! GAAAGACTATATTTAAGGAA+AGG - chr8_3:54420779-54420798 Msa1295930:intron 25.0%
! ATATGGTTGTCTTAAGATTT+AGG - chr8_3:54420875-54420894 Msa1295930:CDS 25.0%
! TATGGTTGTCTTAAGATTTA+GGG - chr8_3:54420876-54420895 Msa1295930:CDS 25.0%
!!! CTAGTAGTAGTAGTTTTATT+TGG - chr8_3:54421014-54421033 Msa1295930:five_prime_UTR 25.0%
!!! TAGTAGTTTTATTTGGTTGT+TGG - chr8_3:54421021-54421040 Msa1295930:five_prime_UTR 25.0%
!!! CTAGGGTTTGTGTATTTTTT+TGG + chr8_3:54418031-54418050 None:intergenic 30.0%
CATTGTCTAAATCAACCATT+GGG + chr8_3:54418207-54418226 None:intergenic 30.0%
ACATTGTCTAAATCAACCAT+TGG + chr8_3:54418208-54418227 None:intergenic 30.0%
!!! GAGATTTCTTTTTTTCTTGG+TGG + chr8_3:54418302-54418321 None:intergenic 30.0%
!!! TGGGAGATTTCTTTTTTTCT+TGG + chr8_3:54418305-54418324 None:intergenic 30.0%
AAGAAAAAAAGAAATCTCCC+AGG - chr8_3:54418304-54418323 Msa1295930:three_prime_UTR 30.0%
GTGTGTGAAATATGTAACAA+AGG - chr8_3:54418480-54418499 Msa1295930:three_prime_UTR 30.0%
AATCTTCAACTACATAGAAG+AGG - chr8_3:54418519-54418538 Msa1295930:three_prime_UTR 30.0%
! AAGCACTTTTGTAGAAAACA+CGG - chr8_3:54418672-54418691 Msa1295930:CDS 30.0%
AGATTGAACAGCATATTTCT+TGG + chr8_3:54418727-54418746 None:intergenic 30.0%
AATATGCTGTTCAATCTGAT+TGG - chr8_3:54418730-54418749 Msa1295930:CDS 30.0%
! AGAGAGTATAAATGTGATTG+TGG - chr8_3:54418780-54418799 Msa1295930:CDS 30.0%
!! TATGTGTTTGATTTTGAAGG+AGG - chr8_3:54418918-54418937 Msa1295930:CDS 30.0%
!! ATGTGTTTGATTTTGAAGGA+GGG - chr8_3:54418919-54418938 Msa1295930:CDS 30.0%
!! GGTGTTATTGTTTGTTTATG+TGG - chr8_3:54419292-54419311 Msa1295930:CDS 30.0%
ATTGTTTGTTTATGTGGTCT+TGG - chr8_3:54419298-54419317 Msa1295930:CDS 30.0%
!! AAATTATGCATTGGTTGTCA+AGG - chr8_3:54419468-54419487 Msa1295930:intron 30.0%
AACACATAGACAAGTAAAGT+TGG + chr8_3:54419599-54419618 None:intergenic 30.0%
! GAAAATTGATGATCCATGAT+TGG + chr8_3:54420047-54420066 None:intergenic 30.0%
GACTAAAGGAATTGATGTTA+GGG - chr8_3:54420338-54420357 Msa1295930:CDS 30.0%
! CAACTACTAGTGTTATTATC+AGG - chr8_3:54420580-54420599 Msa1295930:CDS 30.0%
!!! TATTATCAGGGCAACTTTTT+TGG - chr8_3:54420593-54420612 Msa1295930:CDS 30.0%
!!! TTTTGAAGTTGCAATTGACA+TGG - chr8_3:54420652-54420671 Msa1295930:CDS 30.0%
! TTTGAAGTTGCAATTGACAT+GGG - chr8_3:54420653-54420672 Msa1295930:CDS 30.0%
CATAGTTGCATATTGCATAT+TGG + chr8_3:54420705-54420724 None:intergenic 30.0%
CAATATGCAATATGCAACTA+TGG - chr8_3:54420703-54420722 Msa1295930:intron 30.0%
! AAATTAAGGGTATGTGCATA+TGG - chr8_3:54420858-54420877 Msa1295930:CDS 30.0%
!!! ATGGTCTTGTTTTTGGTTTA+TGG - chr8_3:54420920-54420939 Msa1295930:exon 30.0%
CTAATTTATTCCTACAGCTA+CGG + chr8_3:54420990-54421009 None:intergenic 30.0%
TGGAATTATTGTCACATTTG+TGG - chr8_3:54421041-54421060 Msa1295930:five_prime_UTR 30.0%
!!! CTTTTTTTCTTGGTGGGTTT+TGG + chr8_3:54418295-54418314 None:intergenic 35.0%
TTTGTAGCTAACAACGTTGT+TGG + chr8_3:54418453-54418472 None:intergenic 35.0%
!! AAATCACCTAAAGCTCTAGA+AGG + chr8_3:54418639-54418658 None:intergenic 35.0%
!! AGAGCTTTAGGTGATTTAAC+TGG - chr8_3:54418642-54418661 Msa1295930:CDS 35.0%
!! GAGCTTTAGGTGATTTAACT+GGG - chr8_3:54418643-54418662 Msa1295930:CDS 35.0%
!! AAAGCTCACTCTAAAGTTTG+TGG - chr8_3:54418753-54418772 Msa1295930:CDS 35.0%
!! TCTGATTCAAAGGTTTTGAC+CGG - chr8_3:54419034-54419053 Msa1295930:CDS 35.0%
!!! GACTTGTTTTTGTGAGAAAC+AGG - chr8_3:54419360-54419379 Msa1295930:CDS 35.0%
GGATCAAGTGATATGAGATT+CGG - chr8_3:54419381-54419400 Msa1295930:intron 35.0%
GATCAAGTGATATGAGATTC+GGG - chr8_3:54419382-54419401 Msa1295930:intron 35.0%
ATCAAGTGATATGAGATTCG+GGG - chr8_3:54419383-54419402 Msa1295930:intron 35.0%
ACTTGTCTATGTGTTATGAG+TGG - chr8_3:54419604-54419623 Msa1295930:intron 35.0%
AAGAAAAAGCAAAGCAAAGC+AGG + chr8_3:54419701-54419720 None:intergenic 35.0%
TTGTGGTTCCTCTATGATTT+GGG + chr8_3:54419765-54419784 None:intergenic 35.0%
GTAGTAGTAACAACAACTTG+TGG + chr8_3:54419782-54419801 None:intergenic 35.0%
!!! TGCTTTTTGAAGTAATGCAG+TGG + chr8_3:54420131-54420150 None:intergenic 35.0%
ACTTCAAAAAGCAGCTCAAA+TGG - chr8_3:54420138-54420157 Msa1295930:CDS 35.0%
CTTCAAAAAGCAGCTCAAAT+GGG - chr8_3:54420139-54420158 Msa1295930:CDS 35.0%
GGACTAAAGGAATTGATGTT+AGG - chr8_3:54420337-54420356 Msa1295930:CDS 35.0%
AAATCAAGAGTTGTCTGCTT+AGG + chr8_3:54420382-54420401 None:intergenic 35.0%
AACTCTTGATTTCCTAGGAT+TGG - chr8_3:54420390-54420409 Msa1295930:CDS 35.0%
ACTCTTGATTTCCTAGGATT+GGG - chr8_3:54420391-54420410 Msa1295930:CDS 35.0%
GTCTTTCTTCTGGCATTATA+AGG + chr8_3:54420766-54420785 None:intergenic 35.0%
AGGAAAATGTGACCTTAACA+AGG - chr8_3:54420799-54420818 Msa1295930:intron 35.0%
AAGCACATTGTACCTTGTTA+AGG + chr8_3:54420814-54420833 None:intergenic 35.0%
! AAGGTACAATGTGCTTGTTT+TGG - chr8_3:54420818-54420837 Msa1295930:CDS 35.0%
!!! GAAGATCATGGTCTTGTTTT+TGG - chr8_3:54420913-54420932 Msa1295930:exon 35.0%
CAAATCATGTATTCCTCAGA+TGG - chr8_3:54420954-54420973 Msa1295930:five_prime_UTR 35.0%
TATTCCTCAGATGGATGATT+TGG - chr8_3:54420963-54420982 Msa1295930:five_prime_UTR 35.0%
CGTAGCTGTAGGAATAAATT+AGG - chr8_3:54420988-54421007 Msa1295930:five_prime_UTR 35.0%
!!! TTCTTGGTGGGTTTTGGAAT+TGG + chr8_3:54418289-54418308 None:intergenic 40.0%
! TTTTTGTACCTGGCATTCCT+GGG + chr8_3:54418324-54418343 None:intergenic 40.0%
! ATTTTTGTACCTGGCATTCC+TGG + chr8_3:54418325-54418344 None:intergenic 40.0%
!! TTTTGAAGATCCAGAAGCAG+AGG - chr8_3:54418413-54418432 Msa1295930:three_prime_UTR 40.0%
GAAGAGGACATAATCTACCA+TGG - chr8_3:54418535-54418554 Msa1295930:three_prime_UTR 40.0%
GATCTTTGTCTCAGTTTCCA+TGG + chr8_3:54418555-54418574 None:intergenic 40.0%
!! TGATGAACACAAGTTGGTTC+AGG + chr8_3:54418612-54418631 None:intergenic 40.0%
GGATCATGATGAACACAAGT+TGG + chr8_3:54418618-54418637 None:intergenic 40.0%
!! CATGATCCTTCTAGAGCTTT+AGG - chr8_3:54418630-54418649 Msa1295930:CDS 40.0%
GAAAACACGGTGAGAAAAAG+TGG - chr8_3:54418685-54418704 Msa1295930:CDS 40.0%
!!! ATTGTGGAACCGTTTTTTCC+AGG - chr8_3:54418796-54418815 Msa1295930:CDS 40.0%
! TAGAGCTTTTTGTGATGCGT+TGG - chr8_3:54418958-54418977 Msa1295930:CDS 40.0%
ACTACTCCTCAGTCAAATAG+TGG - chr8_3:54419106-54419125 Msa1295930:CDS 40.0%
!! ATGATTTGGGGTGGATTTTC+TGG + chr8_3:54419752-54419771 None:intergenic 40.0%
AAATCCACCCCAAATCATAG+AGG - chr8_3:54419754-54419773 Msa1295930:intron 40.0%
TGTGGTTCCTCTATGATTTG+GGG + chr8_3:54419764-54419783 None:intergenic 40.0%
CTTGTGGTTCCTCTATGATT+TGG + chr8_3:54419766-54419785 None:intergenic 40.0%
AGTACTAGCTCAACAAGCAA+CGG - chr8_3:54419833-54419852 Msa1295930:intron 40.0%
AGCGAACTAATTAGGTCCAT+GGG - chr8_3:54419950-54419969 Msa1295930:intron 40.0%
! GGATCATCAATTTTCGGAAC+AGG - chr8_3:54420052-54420071 Msa1295930:CDS 40.0%
! TCATCAATTTTCGGAACAGG+AGG - chr8_3:54420055-54420074 Msa1295930:CDS 40.0%
! CATCAATTTTCGGAACAGGA+GGG - chr8_3:54420056-54420075 Msa1295930:CDS 40.0%
ATCTTGTTGACCAGATGAAG+TGG + chr8_3:54420224-54420243 None:intergenic 40.0%
TCAACAAGATAGCCTGCATT+GGG - chr8_3:54420234-54420253 Msa1295930:intron 40.0%
GAAACTAAACTAGAACCCTC+TGG + chr8_3:54420277-54420296 None:intergenic 40.0%
!! GGTTCTAGTTTAGTTTCAGC+AGG - chr8_3:54420280-54420299 Msa1295930:intron 40.0%
! AGTTTAGTTTCAGCAGGACT+TGG - chr8_3:54420286-54420305 Msa1295930:intron 40.0%
TGCTTAGGACCAAACATTGA+CGG + chr8_3:54420367-54420386 None:intergenic 40.0%
CAGACAACTCTTGATTTCCT+AGG - chr8_3:54420385-54420404 Msa1295930:CDS 40.0%
CTCTTGATTTCCTAGGATTG+GGG - chr8_3:54420392-54420411 Msa1295930:CDS 40.0%
TCTGCACTTATCACGTCAAT+TGG - chr8_3:54420448-54420467 Msa1295930:CDS 40.0%
GAGTTCTCTGGCAAAGATAT+TGG - chr8_3:54420523-54420542 Msa1295930:CDS 40.0%
CAACTATGGATCAATGCAAC+AGG - chr8_3:54420717-54420736 Msa1295930:intron 40.0%
TGCAACAGGTAATGTGCAAA+TGG - chr8_3:54420731-54420750 Msa1295930:intron 40.0%
GCAACAGGTAATGTGCAAAT+GGG - chr8_3:54420732-54420751 Msa1295930:intron 40.0%
CAACAGGTAATGTGCAAATG+GGG - chr8_3:54420733-54420752 Msa1295930:intron 40.0%
ACACAGGCTGTGAGAAATTA+AGG - chr8_3:54420844-54420863 Msa1295930:CDS 40.0%
CACAGGCTGTGAGAAATTAA+GGG - chr8_3:54420845-54420864 Msa1295930:CDS 40.0%
GTCTTAAGATTTAGGGACAG+TGG - chr8_3:54420883-54420902 Msa1295930:CDS 40.0%
! AGTGGAGCATTTGAAGATCA+TGG - chr8_3:54420901-54420920 Msa1295930:CDS 40.0%
TTCCACGTTTGTCCATTGTT+TGG - chr8_3:54421066-54421085 Msa1295930:five_prime_UTR 40.0%
ACGCATTAAGTGCCAAACAA+TGG + chr8_3:54421081-54421100 None:intergenic 40.0%
GACAATGTTTCCACAGCTTC+AGG - chr8_3:54418220-54418239 Msa1295930:three_prime_UTR 45.0%
!! TTTTTGTGATGCGTTGGCAG+AGG - chr8_3:54418964-54418983 Msa1295930:CDS 45.0%
GACACACCACTATTTGACTG+AGG + chr8_3:54419115-54419134 None:intergenic 45.0%
CTAATTAGTTCGCTGAACGC+CGG + chr8_3:54419944-54419963 None:intergenic 45.0%
CAGCGAACTAATTAGGTCCA+TGG - chr8_3:54419949-54419968 Msa1295930:intron 45.0%
! ATGATCCATGATTGGTCGAG+AGG + chr8_3:54420039-54420058 None:intergenic 45.0%
TCTTCTGCTTCCACTTCATC+TGG - chr8_3:54420211-54420230 Msa1295930:intron 45.0%
GTCAACAAGATAGCCTGCAT+TGG - chr8_3:54420233-54420252 Msa1295930:intron 45.0%
CAACAAGATAGCCTGCATTG+GGG - chr8_3:54420235-54420254 Msa1295930:intron 45.0%
! TGATGGCGATTCTGGACTAA+AGG - chr8_3:54420324-54420343 Msa1295930:CDS 45.0%
TTAGGGACTCCGTCAATGTT+TGG - chr8_3:54420355-54420374 Msa1295930:CDS 45.0%
TGATTTCCTAGGATTGGGGA+TGG - chr8_3:54420396-54420415 Msa1295930:CDS 45.0%
GCACTTATCACGTCAATTGG+CGG - chr8_3:54420451-54420470 Msa1295930:CDS 45.0%
AGAGAACTCTCCATTACCGA+AGG + chr8_3:54420512-54420531 None:intergenic 45.0%
!! TTCGGTAATGGAGAGTTCTC+TGG - chr8_3:54420511-54420530 Msa1295930:CDS 45.0%
!!! GTGCTTGTTTTGGACAACAC+AGG - chr8_3:54420828-54420847 Msa1295930:CDS 45.0%
!! ATGATTTGGTCCGTAGCTGT+AGG - chr8_3:54420977-54420996 Msa1295930:five_prime_UTR 45.0%
TGCCAAACAATGGACAAACG+TGG + chr8_3:54421071-54421090 None:intergenic 45.0%
GAAGCTAGCATCTCTTCCTC+TGG - chr8_3:54418244-54418263 Msa1295930:three_prime_UTR 50.0%
AGAAATCTCCCAGGAATGCC+AGG - chr8_3:54418313-54418332 Msa1295930:three_prime_UTR 50.0%
AGAGCAATCACCTCTGCTTC+TGG + chr8_3:54418426-54418445 None:intergenic 50.0%
TTTGACCGGTGATTCGAAGC+CGG - chr8_3:54419048-54419067 Msa1295930:CDS 50.0%
GCAGTAGTAGTAATCACCGC+TGG + chr8_3:54419082-54419101 None:intergenic 50.0%
TAGTGGTGTGTCCTCTGCTT+TGG - chr8_3:54419123-54419142 Msa1295930:CDS 50.0%
CTTCTGAGTCTCCAAAGCAG+AGG + chr8_3:54419137-54419156 None:intergenic 50.0%
!! GCTTTGGAGACTCAGAAGCT+AGG - chr8_3:54419139-54419158 Msa1295930:CDS 50.0%
! GGTTCCTCTATGATTTGGGG+TGG + chr8_3:54419761-54419780 None:intergenic 50.0%
CGGCGTTCAGCGAACTAATT+AGG - chr8_3:54419942-54419961 Msa1295930:intron 50.0%
ACGAGGATCTGTACATCCCA+TGG + chr8_3:54419969-54419988 None:intergenic 50.0%
GCTGAGAAATCTGAAGGACG+AGG + chr8_3:54419986-54420005 None:intergenic 50.0%
GGAGGAGCTGAGAAATCTGA+AGG + chr8_3:54419992-54420011 None:intergenic 50.0%
CTTTGCCTCTCGACCAATCA+TGG - chr8_3:54420031-54420050 Msa1295930:CDS 50.0%
! AATTTTCGGAACAGGAGGGC+AGG - chr8_3:54420060-54420079 Msa1295930:CDS 50.0%
GCTGATGTGTAGGCACGTAT+TGG + chr8_3:54420093-54420112 None:intergenic 50.0%
ACAAACGCTTCTCTGCTTCG+TGG - chr8_3:54420172-54420191 Msa1295930:CDS 50.0%
CAAACGCTTCTCTGCTTCGT+GGG - chr8_3:54420173-54420192 Msa1295930:CDS 50.0%
TCTCGGACAAATGGAACCAG+AGG - chr8_3:54420258-54420277 Msa1295930:intron 50.0%
CTCGGACAAATGGAACCAGA+GGG - chr8_3:54420259-54420278 Msa1295930:intron 50.0%
GTTTCAGCAGGACTTGGACT+AGG - chr8_3:54420292-54420311 Msa1295930:intron 50.0%
GGACTAGGTCTTCCTTGTGA+TGG - chr8_3:54420307-54420326 Msa1295930:intron 50.0%
! CTTCCTTGTGATGGCGATTC+TGG - chr8_3:54420316-54420335 Msa1295930:intron 50.0%
AGTCCAGAATCGCCATCACA+AGG + chr8_3:54420322-54420341 None:intergenic 50.0%
CTTATCACGTCAATTGGCGG+TGG - chr8_3:54420454-54420473 Msa1295930:CDS 50.0%
CTCTCCATTACCGAAGGATG+CGG + chr8_3:54420506-54420525 None:intergenic 50.0%
CGGACCAAATCATCCATCTG+AGG + chr8_3:54420970-54420989 None:intergenic 50.0%
! GCTAGCTTCACCTGAAGCTG+TGG + chr8_3:54418233-54418252 None:intergenic 55.0%
TTCTGCGAGTCTCCAACCTC+AGG - chr8_3:54419916-54419935 Msa1295930:intron 55.0%
GAGGCAAAGAGAGATCGCCT+CGG + chr8_3:54420020-54420039 None:intergenic 55.0%
ATCGCAGGAGGCTGATGTGT+AGG + chr8_3:54420103-54420122 None:intergenic 55.0%
AATGCAGTGGCAGACATCGC+AGG + chr8_3:54420118-54420137 None:intergenic 55.0%
GATAGCCTGCATTGGGGTCT+CGG - chr8_3:54420241-54420260 Msa1295930:intron 55.0%
TTTGTCCGAGACCCCAATGC+AGG + chr8_3:54420249-54420268 None:intergenic 55.0%
GCATTGGGGTCTCGGACAAA+TGG - chr8_3:54420249-54420268 Msa1295930:intron 55.0%
AAGTGCAGACAAGCCTCCAC+CGG + chr8_3:54420437-54420456 None:intergenic 55.0%
!! CGACACCGGCTTCGAATCAC+CGG + chr8_3:54419056-54419075 None:intergenic 60.0%
!! ATCACCGCTGGCATCGACAC+CGG + chr8_3:54419070-54419089 None:intergenic 60.0%
! GGCTGTGCTACTACCAGCAC+TGG - chr8_3:54419854-54419873 Msa1295930:intron 60.0%
GAGTCTCCAACCTCAGGCAC+CGG - chr8_3:54419922-54419941 Msa1295930:intron 60.0%
AGAGAGATCGCCTCGGACGA+TGG + chr8_3:54420013-54420032 None:intergenic 60.0%
GCTTCTCTGCTTCGTGGGCT+TGG - chr8_3:54420178-54420197 Msa1295930:CDS 60.0%
CAGCAGCCATCCCCAATCCT+AGG + chr8_3:54420405-54420424 None:intergenic 60.0%
CTAGGATTGGGGATGGCTGC+TGG - chr8_3:54420403-54420422 Msa1295930:CDS 60.0%
! ATGGCTGCTGGTGGTAGTGC+CGG - chr8_3:54420415-54420434 Msa1295930:CDS 60.0%
ACGTCAATTGGCGGTGGCAG+TGG - chr8_3:54420460-54420479 Msa1295930:CDS 60.0%
AATTGGCGGTGGCAGTGGTC+TGG - chr8_3:54420465-54420484 Msa1295930:CDS 60.0%
GCAGCCGCATCCTTCGGTAA+TGG - chr8_3:54420499-54420518 Msa1295930:CDS 60.0%
!! GAAGCCGGTGTCGATGCCAG+CGG - chr8_3:54419063-54419082 Msa1295930:CDS 65.0%
GCTCGCAAACACGCCAGTGC+TGG + chr8_3:54419870-54419889 None:intergenic 65.0%
TGAACGCCGGTGCCTGAGGT+TGG + chr8_3:54419931-54419950 None:intergenic 65.0%
CTCAGCTCCTCCATCGTCCG+AGG - chr8_3:54420000-54420019 Msa1295930:CDS 65.0%
GAGATCGCCTCGGACGATGG+AGG + chr8_3:54420010-54420029 None:intergenic 65.0%
GCAGTGGCAGACATCGCAGG+AGG + chr8_3:54420115-54420134 None:intergenic 65.0%
GGATTGGGGATGGCTGCTGG+TGG - chr8_3:54420406-54420425 Msa1295930:CDS 65.0%
ACCGCAGCAGCCGCATCCTT+CGG - chr8_3:54420493-54420512 Msa1295930:CDS 65.0%
ACCGAAGGATGCGGCTGCTG+CGG + chr8_3:54420497-54420516 None:intergenic 65.0%
TCGCTGAACGCCGGTGCCTG+AGG + chr8_3:54419935-54419954 None:intergenic 70.0%
! GCTGCTGGTGGTAGTGCCGG+TGG - chr8_3:54420418-54420437 Msa1295930:CDS 70.0%
!! GCTGGTGGTAGTGCCGGTGG+AGG - chr8_3:54420421-54420440 Msa1295930:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 54418002 54421132 54418002 ID=Msa1295930;Name=Msa1295930
chr8_3 mRNA 54418002 54421132 54418002 ID=Msa1295930-mRNA-1;Parent=Msa1295930;Name=Msa1295930-mRNA-1;_AED=0.00;_eAED=0.00;_QI=206|1|1|1|1|1|4|574|520
chr8_3 exon 54418002 54419390 54418002 ID=Msa1295930-mRNA-1:exon:15664;Parent=Msa1295930-mRNA-1
chr8_3 exon 54419974 54420197 54419974 ID=Msa1295930-mRNA-1:exon:15663;Parent=Msa1295930-mRNA-1
chr8_3 exon 54420317 54420713 54420317 ID=Msa1295930-mRNA-1:exon:15662;Parent=Msa1295930-mRNA-1
chr8_3 exon 54420800 54421132 54420800 ID=Msa1295930-mRNA-1:exon:15661;Parent=Msa1295930-mRNA-1
chr8_3 five_prime_UTR 54420927 54421132 54420927 ID=Msa1295930-mRNA-1:five_prime_utr;Parent=Msa1295930-mRNA-1
chr8_3 CDS 54420800 54420926 54420800 ID=Msa1295930-mRNA-1:cds;Parent=Msa1295930-mRNA-1
chr8_3 CDS 54420317 54420713 54420317 ID=Msa1295930-mRNA-1:cds;Parent=Msa1295930-mRNA-1
chr8_3 CDS 54419974 54420197 54419974 ID=Msa1295930-mRNA-1:cds;Parent=Msa1295930-mRNA-1
chr8_3 CDS 54418576 54419390 54418576 ID=Msa1295930-mRNA-1:cds;Parent=Msa1295930-mRNA-1
chr8_3 three_prime_UTR 54418002 54418575 54418002 ID=Msa1295930-mRNA-1:three_prime_utr;Parent=Msa1295930-mRNA-1
Gene Sequence

>Msa1295930

ATGGTTGATTTAGACAATGTTTCCACAGCTTCAGGTGAAGCTAGCATCTCTTCCTCTGGTAATAACAATATTCAATCACCAATTCCAAAACCCACCAAGAAAAAAAGAAATCTCCCAGGAATGCCAGATCCAGAAGCAGAGGTGATTGCTCTGTCACCAACAACGTTGTTAGCTACAAACAGATTTGTGTGTGAAATATGTAACAAAGGGTTTCAAAGAGATCAGAATCTTCAACTACATAGAAGAGGACATAATCTACCATGGAAACTGAGACAAAGATCAAGTAAAGAAATTAGGAAAAGGGTTTATGTTTGTCCTGAACCAACTTGTGTTCATCATGATCCTTCTAGAGCTTTAGGTGATTTAACTGGGATAAAAAAGCACTTTTGTAGAAAACACGGTGAGAAAAAGTGGAAATGTGAGAAATGTTCCAAGAAATATGCTGTTCAATCTGATTGGAAAGCTCACTCTAAAGTTTGTGGTTCTAGAGAGTATAAATGTGATTGTGGAACCGTTTTTTCCAGGAGGGATAGTTTTATCACACATAGAGCTTTTTGTGATGCGTTGGCAGAGGAGAATGCAAAATCTCAGAATCAAGCTGTTGTGAAAGCTAATTCAGAATCTGATTCAAAGGTTTTGACCGGTGATTCGAAGCCGGTGTCGATGCCAGCGGTGATTACTACTACTGCTGCTACTACTCCTCAGTCAAATAGTGGTGTGTCCTCTGCTTTGGAGACTCAGAAGCTAGATTTGCCAGAAAATCCACCCCAAATCATAGAGGAACCACAAGTTGTTGTTACTACTACTGCTAGAGCTTTAAATGCAAGTTGTAGCAGCAGTACTAGCTCAACAAGCAACGGCTGTGCTACTACCAGCACTGGCGTGTTTGCGAGCTTGTTTGCTTCATCAACAGCTTCAGCTTCTGCGAGTCTCCAACCTCAGGCACCGGCGTTCAGCGAACTAATTAGGTCCATGGGATGTACAGATCCTCGTCCTTCAGATTTCTCAGCTCCTCCATCGTCCGAGGCGATCTCTCTTTGCCTCTCGACCAATCATGGATCATCAATTTTCGGAACAGGAGGGCAGGAGTGTCGCCAATACGTGCCTACACATCAGCCTCCTGCGATGTCTGCCACTGCATTACTTCAAAAAGCAGCTCAAATGGGCGCTGCTGCAACAAACGCTTCTCTGCTTCGTGGGCTTGGAATTGTGTCGTCTTCTGCTTCCACTTCATCTGGTCAACAAGATAGCCTGCATTGGGGTCTCGGACAAATGGAACCAGAGGGTTCTAGTTTAGTTTCAGCAGGACTTGGACTAGGTCTTCCTTGTGATGGCGATTCTGGACTAAAGGAATTGATGTTAGGGACTCCGTCAATGTTTGGTCCTAAGCAGACAACTCTTGATTTCCTAGGATTGGGGATGGCTGCTGGTGGTAGTGCCGGTGGAGGCTTGTCTGCACTTATCACGTCAATTGGCGGTGGCAGTGGTCTGGATGTTACCGCAGCAGCCGCATCCTTCGGTAATGGAGAGTTCTCTGGCAAAGATATTGGAAGAAGTTCATGA
Protein sequence

>Msa1295930

MVDLDNVSTASGEASISSSGNNNIQSPIPKPTKKKRNLPGMPDPEAEVIALSPTTLLATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLRQRSSKEIRKRVYVCPEPTCVHHDPSRALGDLTGIKKHFCRKHGEKKWKCEKCSKKYAVQSDWKAHSKVCGSREYKCDCGTVFSRRDSFITHRAFCDALAEENAKSQNQAVVKANSESDSKVLTGDSKPVSMPAVITTTAATTPQSNSGVSSALETQKLDLPENPPQIIEEPQVVVTTTARALNASCSSSTSSTSNGCATTSTGVFASLFASSTASASASLQPQAPAFSELIRSMGCTDPRPSDFSAPPSSEAISLCLSTNHGSSIFGTGGQECRQYVPTHQPPAMSATALLQKAAQMGAAATNASLLRGLGIVSSSASTSSGQQDSLHWGLGQMEPEGSSLVSAGLGLGLPCDGDSGLKELMLGTPSMFGPKQTTLDFLGLGMAAGGSAGGGLSALITSIGGGSGLDVTAAAASFGNGEFSGKDIGRSS*