AlfalfaGEDB | Msa1209240

M. sativa cultivar zhongmu-4 / Msa1209240

KEGG: K06195
GO: -
PFAM: PF02151.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1209240	XP_013457802.1	97.015	268	6	1	1	268	1	266	0.0	533

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1209240	sp\|B0UC46\|APAG_METS4	52.500	120	54	1	138	257	4	120	1.29e-33	122

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0016830	Msa1209240	0.801408	9.132643e-49	-8.615850e-47
Msa0028410	Msa1209240	0.830643	2.616441e-55	-8.615850e-47
Msa0039920	Msa1209240	0.832607	8.585454e-56	-8.615850e-47
Msa0040090	Msa1209240	0.811696	6.151492e-51	-8.615850e-47
Msa0042210	Msa1209240	0.807675	4.501398e-50	-8.615850e-47
Msa0046050	Msa1209240	0.841449	4.721363e-58	-8.615850e-47
Msa0057740	Msa1209240	0.817526	3.150352e-52	-8.615850e-47
Msa0065190	Msa1209240	0.811363	7.267708e-51	-8.615850e-47
Msa0080620	Msa1209240	0.862274	5.691797e-64	-8.615850e-47
Msa0087580	Msa1209240	0.823091	1.669563e-53	-8.615850e-47
Msa0087780	Msa1209240	0.811176	7.980859e-51	-8.615850e-47
Msa0087790	Msa1209240	0.814999	1.157361e-51	-8.615850e-47
Msa0092750	Msa1209240	0.811317	7.439320e-51	-8.615850e-47
Msa0116950	Msa1209240	0.812220	4.731557e-51	-8.615850e-47
Msa0620050	Msa1209240	0.800110	1.680685e-48	-8.615850e-47
Msa0624260	Msa1209240	0.817686	2.899879e-52	-8.615850e-47
Msa0624360	Msa1209240	0.819667	1.029793e-52	-8.615850e-47
Msa0630090	Msa1209240	0.801555	8.520151e-49	-8.615850e-47
Msa0648810	Msa1209240	0.803316	3.695086e-49	-8.615850e-47
Msa0653690	Msa1209240	0.821624	3.656754e-53	-8.615850e-47
Msa0669710	Msa1209240	0.821802	3.325816e-53	-8.615850e-47
Msa0669750	Msa1209240	0.824890	6.317323e-54	-8.615850e-47
Msa0677110	Msa1209240	0.842346	2.734745e-58	-8.615850e-47
Msa0677250	Msa1209240	0.845156	4.841196e-59	-8.615850e-47
Msa0679270	Msa1209240	0.806113	9.630786e-50	-8.615850e-47
Msa0699200	Msa1209240	0.820593	6.318392e-53	-8.615850e-47
Msa0705050	Msa1209240	0.800186	1.621971e-48	-8.615850e-47
Msa0705130	Msa1209240	0.803530	3.336213e-49	-8.615850e-47
Msa0705810	Msa1209240	0.808755	2.650581e-50	-8.615850e-47
Msa0706300	Msa1209240	0.801052	1.080244e-48	-8.615850e-47
Msa0711860	Msa1209240	0.836526	8.885749e-57	-8.615850e-47
Msa0712670	Msa1209240	0.802606	5.179126e-49	-8.615850e-47
Msa0714200	Msa1209240	0.812702	3.712530e-51	-8.615850e-47
Msa0714230	Msa1209240	0.802308	5.967030e-49	-8.615850e-47
Msa0744280	Msa1209240	0.847828	9.031747e-60	-8.615850e-47
Msa0754840	Msa1209240	0.802472	5.520313e-49	-8.615850e-47
Msa0756440	Msa1209240	0.816783	4.628434e-52	-8.615850e-47
Msa0770540	Msa1209240	0.805531	1.276518e-49	-8.615850e-47
Msa0799150	Msa1209240	0.847786	9.278854e-60	-8.615850e-47
Msa0807840	Msa1209240	0.824802	6.625913e-54	-8.615850e-47
Msa0812120	Msa1209240	0.818758	1.658501e-52	-8.615850e-47
Msa0825520	Msa1209240	0.814556	1.450710e-51	-8.615850e-47
Msa0833910	Msa1209240	0.823520	1.325104e-53	-8.615850e-47
Msa0124070	Msa1209240	0.814158	1.776454e-51	-8.615850e-47
Msa0134300	Msa1209240	0.821708	3.497590e-53	-8.615850e-47
Msa0134380	Msa1209240	0.801520	8.661813e-49	-8.615850e-47
Msa0134400	Msa1209240	0.817468	3.246433e-52	-8.615850e-47
Msa0134420	Msa1209240	0.824692	7.032659e-54	-8.615850e-47
Msa0137010	Msa1209240	0.828009	1.140541e-54	-8.615850e-47
Msa0160470	Msa1209240	0.814761	1.306528e-51	-8.615850e-47
Msa0168470	Msa1209240	0.814067	1.860240e-51	-8.615850e-47
Msa0176660	Msa1209240	0.833210	6.077145e-56	-8.615850e-47
Msa0180590	Msa1209240	0.800422	1.452175e-48	-8.615850e-47
Msa0180600	Msa1209240	0.843066	1.761152e-58	-8.615850e-47
Msa0180780	Msa1209240	0.818383	2.016889e-52	-8.615850e-47
Msa0186510	Msa1209240	0.808514	2.983516e-50	-8.615850e-47
Msa0186750	Msa1209240	0.800202	1.610053e-48	-8.615850e-47
Msa0197600	Msa1209240	0.800943	1.136775e-48	-8.615850e-47
Msa0213020	Msa1209240	0.830479	2.869352e-55	-8.615850e-47
Msa0230980	Msa1209240	0.809533	1.805372e-50	-8.615850e-47
Msa0379090	Msa1209240	0.801533	8.610933e-49	-8.615850e-47
Msa0424220	Msa1209240	0.805103	1.568668e-49	-8.615850e-47
Msa0424940	Msa1209240	0.806180	9.322396e-50	-8.615850e-47
Msa0426860	Msa1209240	0.818369	2.032033e-52	-8.615850e-47
Msa0426890	Msa1209240	0.810189	1.304119e-50	-8.615850e-47
Msa0430040	Msa1209240	0.811226	7.784127e-51	-8.615850e-47
Msa0437790	Msa1209240	0.848514	5.840197e-60	-8.615850e-47
Msa0456480	Msa1209240	0.812082	5.070139e-51	-8.615850e-47
Msa0469770	Msa1209240	0.822127	2.797182e-53	-8.615850e-47
Msa0469840	Msa1209240	0.822376	2.449258e-53	-8.615850e-47
Msa1019370	Msa1209240	0.817303	3.535911e-52	-8.615850e-47
Msa1030110	Msa1209240	0.832941	7.091425e-56	-8.615850e-47
Msa1036020	Msa1209240	-0.801001	1.106333e-48	-8.615850e-47
Msa1047390	Msa1209240	0.814307	1.646708e-51	-8.615850e-47
Msa1057320	Msa1209240	0.826577	2.513294e-54	-8.615850e-47
Msa1057340	Msa1209240	0.824874	6.370621e-54	-8.615850e-47
Msa1088740	Msa1209240	0.815680	8.167748e-52	-8.615850e-47
Msa0270460	Msa1209240	0.818304	2.101534e-52	-8.615850e-47
Msa0293000	Msa1209240	0.815220	1.033819e-51	-8.615850e-47
Msa0293060	Msa1209240	0.818851	1.579638e-52	-8.615850e-47
Msa0317630	Msa1209240	0.804094	2.547579e-49	-8.615850e-47
Msa0321230	Msa1209240	0.801606	8.316297e-49	-8.615850e-47
Msa0322480	Msa1209240	0.814469	1.516234e-51	-8.615850e-47
Msa0335860	Msa1209240	0.815937	7.158070e-52	-8.615850e-47
Msa0355990	Msa1209240	0.826068	3.322060e-54	-8.615850e-47
Msa1128850	Msa1209240	0.895448	9.490408e-76	-8.615850e-47
Msa1135910	Msa1209240	0.807042	6.130299e-50	-8.615850e-47
Msa1140480	Msa1209240	0.811788	5.874654e-51	-8.615850e-47
Msa1145430	Msa1209240	0.801005	1.104002e-48	-8.615850e-47
Msa1190300	Msa1209240	0.820948	5.236164e-53	-8.615850e-47
Msa1190320	Msa1209240	0.824759	6.780548e-54	-8.615850e-47
Msa1202220	Msa1209240	0.812303	4.537955e-51	-8.615850e-47
Msa1203840	Msa1209240	0.802105	6.567892e-49	-8.615850e-47
Msa1203860	Msa1209240	0.845131	4.915534e-59	-8.615850e-47
Msa1209240	Msa1224310	0.804954	1.685826e-49	-8.615850e-47
Msa1209240	Msa1249740	0.809924	1.487743e-50	-8.615850e-47
Msa1209240	Msa1270850	0.800015	1.757003e-48	-8.615850e-47
Msa1209240	Msa1270860	0.818419	1.979642e-52	-8.615850e-47
Msa1209240	Msa1286660	0.803823	2.900344e-49	-8.615850e-47
Msa1209240	Msa1292700	0.817419	3.330615e-52	-8.615850e-47
Msa1209240	Msa1295480	0.806489	8.025889e-50	-8.615850e-47
Msa1209240	Msa1296270	0.815318	9.830086e-52	-8.615850e-47
Msa1209240	Msa1308010	0.814309	1.644943e-51	-8.615850e-47
Msa1209240	Msa1309910	0.803839	2.878636e-49	-8.615850e-47
Msa1209240	Msa1330080	0.805341	1.398703e-49	-8.615850e-47
Msa1209240	Msa1330910	0.832088	1.154178e-55	-8.615850e-47
Msa1209240	Msa1335200	0.805216	1.485702e-49	-8.615850e-47
Msa1209240	Msa1335210	0.819929	8.972240e-53	-8.615850e-47
Msa1209240	Msa1349850	0.817971	2.500461e-52	-8.615850e-47
Msa1209240	Msa1350350	0.821933	3.102840e-53	-8.615850e-47
Msa1209240	Msa1356500	0.817687	2.897748e-52	-8.615850e-47
Msa1209240	Msa1358790	0.812385	4.353608e-51	-8.615850e-47
Msa1209240	Msa1364850	0.811690	6.170426e-51	-8.615850e-47
Msa1209240	Msa1369390	0.824277	8.805724e-54	-8.615850e-47
Msa1209240	Msa1369400	0.805203	1.495016e-49	-8.615850e-47
Msa1209240	Msa1373390	0.831724	1.419069e-55	-8.615850e-47
Msa1209240	Msa1384620	0.813647	2.302891e-51	-8.615850e-47
Msa1209240	Msa1388070	0.808311	3.295674e-50	-8.615850e-47
Msa1209240	Msa1388270	0.807453	5.016527e-50	-8.615850e-47
Msa1209240	Msa1420600	0.860774	1.633990e-63	-8.615850e-47
Msa1209240	Msa1425540	0.847225	1.323195e-59	-8.615850e-47
Msa1209240	Msa1427020	0.827140	1.843863e-54	-8.615850e-47
Msa1209240	Msa1430630	0.807507	4.887183e-50	-8.615850e-47
Msa1209240	Msa1435690	0.813920	2.004504e-51	-8.615850e-47
Msa0490740	Msa1209240	0.818384	2.016347e-52	-8.615850e-47
Msa0511600	Msa1209240	0.846709	1.831717e-59	-8.615850e-47
Msa0512930	Msa1209240	0.821625	3.654954e-53	-8.615850e-47
Msa0532880	Msa1209240	0.807392	5.170030e-50	-8.615850e-47
Msa0537960	Msa1209240	0.814401	1.569815e-51	-8.615850e-47
Msa0552670	Msa1209240	0.817874	2.630154e-52	-8.615850e-47
Msa0562040	Msa1209240	0.803834	2.885379e-49	-8.615850e-47
Msa0565930	Msa1209240	0.804047	2.605526e-49	-8.615850e-47
Msa0566090	Msa1209240	0.824981	6.010227e-54	-8.615850e-47
Msa0581240	Msa1209240	0.988264	2.416755e-173	-8.615850e-47
Msa0595780	Msa1209240	0.810435	1.154142e-50	-8.615850e-47
Msa0598470	Msa1209240	0.821000	5.095356e-53	-8.615850e-47
Msa0848860	Msa1209240	0.815394	9.455393e-52	-8.615850e-47
Msa0852370	Msa1209240	0.821697	3.518449e-53	-8.615850e-47
Msa0864730	Msa1209240	0.817559	3.097269e-52	-8.615850e-47
Msa0864750	Msa1209240	0.814676	1.364870e-51	-8.615850e-47
Msa0866510	Msa1209240	0.844474	7.393288e-59	-8.615850e-47
Msa0876030	Msa1209240	0.826000	3.449096e-54	-8.615850e-47
Msa0930950	Msa1209240	0.805657	1.201169e-49	-8.615850e-47
Msa0932040	Msa1209240	0.812743	3.635922e-51	-8.615850e-47
Msa0961200	Msa1209240	0.826988	2.005434e-54	-8.615850e-47
Msa0967540	Msa1209240	0.822759	1.994217e-53	-8.615850e-47
Msa0970160	Msa1209240	0.814453	1.528531e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1209240	MtrunA17_Chr4g0058961	97.015	268	6	1	1	268	1	266	0.0	533

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 105 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATTGATGGTGTTTATAATT+TGG	0.117504	8_1:+67682481	Msa1209240:three_prime_UTR
CTACCATTTGGTGTGTTTAA+TGG	0.187250	8_1:-67681490	None:intergenic
CCAAGCAGAGCGAGAGAAAA+TGG	0.241394	8_1:-67682170	None:intergenic
GTTTGGAATTGGGTGGTTAA+TGG	0.263795	8_1:-67678799	None:intergenic
ATACTCCATGTGCTAATAAA+TGG	0.273729	8_1:-67682252	None:intergenic
GGAGTACTCAAAACTAGTTC+TGG	0.277502	8_1:-67681458	None:intergenic
ATGTATTCTCATAATGGTTT+TGG	0.281377	8_1:-67682304	None:intergenic
AACGGTGTCATCTGAGTTAT+TGG	0.282416	8_1:-67681130	None:intergenic
TACCATTTGGTGTGTTTAAT+GGG	0.285236	8_1:-67681489	None:intergenic
ATTTATTAGCACATGGAGTA+TGG	0.298662	8_1:+67682254	Msa1209240:three_prime_UTR
CAGAAAATGTATTCTCATAA+TGG	0.310803	8_1:-67682310	None:intergenic
CACGACAAGGACTAAGTCTA+AGG	0.315715	8_1:+67678737	Msa1209240:five_prime_UTR
TTCATCTTATCTAGGGAGTC+AGG	0.329309	8_1:+67681025	Msa1209240:intron
GGGTATGTATGGTTTGGAAT+TGG	0.341213	8_1:-67678810	None:intergenic
GCGTTTAAGGAGGCTAATTA+AGG	0.341665	8_1:+67679271	Msa1209240:CDS
ACACCGTTCAACTTCTTAGA+AGG	0.345950	8_1:+67681145	Msa1209240:CDS
TTTGGCGGGTTTGCGGTTGA+TGG	0.351768	8_1:+67678934	Msa1209240:CDS
GGTATGTATGGTTTGGAATT+GGG	0.369330	8_1:-67678809	None:intergenic
GTTAAGTAGTACCAAGAGTT+TGG	0.384169	8_1:+67678916	Msa1209240:CDS
ATCCTGTTCGCGGGTATGTA+TGG	0.390875	8_1:-67678821	None:intergenic
TTTGAACATTAGCACTGTTA+AGG	0.394687	8_1:+67682606	Msa1209240:three_prime_UTR
GTTCTTACCATTCTACCATT+TGG	0.394756	8_1:-67681502	None:intergenic
AATGCTATGTGCTTATAGTT+TGG	0.398142	8_1:-67682585	None:intergenic
AGCCTCCTTAAACGCAAAAC+AGG	0.417473	8_1:-67679263	None:intergenic
CTAGTTCTGGGAAGAAGTAC+TGG	0.429029	8_1:-67681445	None:intergenic
CCAAGAGTTTGGCGGGTTTG+CGG	0.430302	8_1:+67678927	Msa1209240:CDS
TGAAATCCTAAGTTACTAAT+CGG	0.437849	8_1:-67678761	None:intergenic
TTGAAAAGTGAATCAATTGA+TGG	0.451618	8_1:+67682467	Msa1209240:three_prime_UTR
AACCATACATACCCGCGAAC+AGG	0.451651	8_1:+67678819	Msa1209240:five_prime_UTR
GTTCGCGGGTATGTATGGTT+TGG	0.453981	8_1:-67678816	None:intergenic
TGCTCTGTTGAAACAACAAA+TGG	0.454031	8_1:+67679087	Msa1209240:CDS
CCGCAAACCCGCCAAACTCT+TGG	0.454081	8_1:-67678927	None:intergenic
GGTGGCATGTAGTAATAATT+CGG	0.455634	8_1:+67679012	Msa1209240:CDS
CAAGCAGAGCGAGAGAAAAT+GGG	0.456582	8_1:-67682169	None:intergenic
GTTGGTTTGCCTTGCGCTCA+CGG	0.466031	8_1:+67682528	Msa1209240:three_prime_UTR
AGGGAGTCAGGGTTCAAGTC+AGG	0.469382	8_1:+67681037	Msa1209240:intron
TTGGCGGGTTTGCGGTTGAT+GGG	0.472771	8_1:+67678935	Msa1209240:CDS
GGATCATAACCGATGCTAAC+GGG	0.473221	8_1:+67681172	Msa1209240:CDS
GATAGAAATGAGGTTGAACT+TGG	0.475178	8_1:-67679049	None:intergenic
AGTCAGGAGTGTATATATAG+AGG	0.482751	8_1:+67681053	Msa1209240:CDS
GCTTAAGTTGTTTGAAGAAG+AGG	0.484114	8_1:+67679238	Msa1209240:CDS
GTTCAACCTCATTTCTATCT+CGG	0.485915	8_1:+67679053	Msa1209240:CDS
GAGTACTCAAAACTAGTTCT+GGG	0.486792	8_1:-67681457	None:intergenic
AATTGCTGATGAGAGATTCC+AGG	0.487924	8_1:+67679298	Msa1209240:CDS
CAGGATCAGTGAGGACGATA+AGG	0.493142	8_1:+67678838	Msa1209240:five_prime_UTR
TAAGGGCCGATTAGTAACTT+AGG	0.496752	8_1:+67678755	Msa1209240:five_prime_UTR
TTCTGATCTCCCGTTAGCAT+CGG	0.500995	8_1:-67681181	None:intergenic
TTCAACTTCTTAGAAGGCAC+TGG	0.506775	8_1:+67681151	Msa1209240:CDS
GAACAACCATGTAGAGGAAG+TGG	0.509128	8_1:+67682503	Msa1209240:three_prime_UTR
ATGATCCATGTTGATAAAGT+AGG	0.511158	8_1:+67682119	Msa1209240:CDS
TACCAACGTTGTAGCATCAC+TGG	0.515982	8_1:-67680433	None:intergenic
TGCTGCAGTTCAATCTCAAA+TGG	0.516025	8_1:-67682210	None:intergenic
TGCCCATTAAACACACCAAA+TGG	0.516035	8_1:+67681487	Msa1209240:CDS
CATGTAGAGGAAGTGGATGT+TGG	0.516444	8_1:+67682510	Msa1209240:three_prime_UTR
CCTTGTCGTGAGGGTATTCA+CGG	0.521240	8_1:-67678724	None:intergenic
AAACACACCAAATGGTAGAA+TGG	0.525368	8_1:+67681495	Msa1209240:CDS
GGGAAGAAGTCAGCCTTCGA+AGG	0.529071	8_1:+67681074	Msa1209240:CDS
TTTATTAGCACATGGAGTAT+GGG	0.531333	8_1:+67682255	Msa1209240:three_prime_UTR
GTCCTCACTGATCCTGTTCG+CGG	0.533143	8_1:-67678831	None:intergenic
GTCAAGAAGCGAGGCTGAAG+AGG	0.534437	8_1:+67678957	Msa1209240:CDS
CAACATCCACTTCCTCTACA+TGG	0.535643	8_1:-67682509	None:intergenic
AAGAAGCGAGGCTGAAGAGG+AGG	0.542369	8_1:+67678960	Msa1209240:CDS
AAGTAGTACCAAGAGTTTGG+CGG	0.543463	8_1:+67678919	Msa1209240:CDS
AGTAGTACCAAGAGTTTGGC+GGG	0.546540	8_1:+67678920	Msa1209240:CDS
GGAAGAAGTCAGCCTTCGAA+GGG	0.546891	8_1:+67681075	Msa1209240:CDS
TAGACTTAGTCCTTGTCGTG+AGG	0.547003	8_1:-67678734	None:intergenic
GTCAGGAGTGTATATATAGA+GGG	0.549148	8_1:+67681054	Msa1209240:CDS
TAAATGGACACAACCTCTGA+AGG	0.554586	8_1:-67682236	None:intergenic
ATGTATGGTTTGGAATTGGG+TGG	0.556121	8_1:-67678806	None:intergenic
AACTGCAGCATCACCTTCAG+AGG	0.556856	8_1:+67682223	Msa1209240:three_prime_UTR
TTGTTGTTTCAACAGAGCAT+AGG	0.559108	8_1:-67679083	None:intergenic
TCATCTTATCTAGGGAGTCA+GGG	0.560049	8_1:+67681026	Msa1209240:intron
AGCATTGTTATAGGATTACA+AGG	0.560344	8_1:+67679193	Msa1209240:intron
AGAAGCGAGGCTGAAGAGGA+GGG	0.560783	8_1:+67678961	Msa1209240:CDS
AGACTTAGTCCTTGTCGTGA+GGG	0.566194	8_1:-67678733	None:intergenic
CAATAGCAATAGCAATATCA+TGG	0.568801	8_1:+67678988	Msa1209240:CDS
GCGAGGCTGAAGAGGAGGGG+TGG	0.571122	8_1:+67678965	Msa1209240:CDS
TGGCTACAACCGTGAGCGCA+AGG	0.572957	8_1:-67682537	None:intergenic
GCAATGATCGCACTAAATGA+AGG	0.577136	8_1:-67682146	None:intergenic
CAATAGCAATATCATGGTGG+TGG	0.578035	8_1:+67678994	Msa1209240:CDS
TCCTCACTGATCCTGTTCGC+GGG	0.578364	8_1:-67678830	None:intergenic
GGAAGGATTATCGTTCTCAC+TGG	0.583244	8_1:+67682281	Msa1209240:three_prime_UTR
ACATGGAGTATGGGAAAGGA+AGG	0.587667	8_1:+67682264	Msa1209240:three_prime_UTR
TGTGTCCATTTATTAGCACA+TGG	0.592435	8_1:+67682247	Msa1209240:three_prime_UTR
TAGCACATGGAGTATGGGAA+AGG	0.598219	8_1:+67682260	Msa1209240:three_prime_UTR
GAAGCGAGGCTGAAGAGGAG+GGG	0.600797	8_1:+67678962	Msa1209240:CDS
ACGGGAGATCAGAAAATGTA+CGG	0.603434	8_1:+67681190	Msa1209240:CDS
ACATTAGCACTGTTAAGGTA+CGG	0.604652	8_1:+67682611	Msa1209240:three_prime_UTR
TTCCAGTGATGCTACAACGT+TGG	0.604919	8_1:+67680431	Msa1209240:CDS
TGGATCATAACCGATGCTAA+CGG	0.617395	8_1:+67681171	Msa1209240:CDS
AAAGTAAAACGAACATAACG+TGG	0.619728	8_1:-67682664	None:intergenic
CCGTGAATACCCTCACGACA+AGG	0.633405	8_1:+67678724	Msa1209240:five_prime_UTR
GTGAGCCTACTTTATCAACA+TGG	0.633871	8_1:-67682124	None:intergenic
TTGAGTCCGAGATAGAAATG+AGG	0.635687	8_1:-67679059	None:intergenic
GTGCCTTCTAAGAAGTTGAA+CGG	0.636127	8_1:-67681148	None:intergenic
GGAAGTAGCTGCTAAATCTG+AGG	0.636191	8_1:+67679108	Msa1209240:CDS
ACGACAAGGACTAAGTCTAA+GGG	0.636404	8_1:+67678738	Msa1209240:five_prime_UTR
AATTTGGAACAACCATGTAG+AGG	0.642339	8_1:+67682497	Msa1209240:three_prime_UTR
AAGCAGAGCGAGAGAAAATG+GGG	0.642561	8_1:-67682168	None:intergenic
GGTTGATGGGTCAAGAAGCG+AGG	0.667813	8_1:+67678948	Msa1209240:CDS
TGTGTTTAATGGGCATGCAG+AGG	0.674221	8_1:-67681479	None:intergenic
AAGTAAAACGAACATAACGT+GGG	0.695187	8_1:-67682663	None:intergenic
GAAGAAGTCAGCCTTCGAAG+GGG	0.718520	8_1:+67681076	Msa1209240:CDS
TAGCAATAGCAATATCATGG+TGG	0.752346	8_1:+67678991	Msa1209240:CDS
ACCCGCGAACAGGATCAGTG+AGG	0.787875	8_1:+67678829	Msa1209240:five_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTATGAAGATCTTTAA+TGG	-	chr8_1:67680926-67680945	None:intergenic	15.0%
!!	AGATCTTCATAAAAAAAAAA+AGG	+	chr8_1:67680930-67680949	Msa1209240:intron	15.0%
!!!	ATTTAATTTCATCTTATCTA+GGG	+	chr8_1:67681018-67681037	Msa1209240:intron	15.0%
!!!	TGCTTTTCTTTTTTATAATA+TGG	-	chr8_1:67681787-67681806	None:intergenic	15.0%
!!!	AAAAGGAGTATATTTTGAAA+GGG	-	chr8_1:67679376-67679395	None:intergenic	20.0%
!!	AATGCATTACAATATTCAAT+AGG	+	chr8_1:67679454-67679473	Msa1209240:intron	20.0%
!!	TTAAGTGTACAAAAAATACA+AGG	-	chr8_1:67679556-67679575	None:intergenic	20.0%
!!	ACTTAATTAAAAATTGTGTG+TGG	+	chr8_1:67679570-67679589	Msa1209240:intron	20.0%
!!	CTTAATTAAAAATTGTGTGT+GGG	+	chr8_1:67679571-67679590	Msa1209240:intron	20.0%
!!	AAATTTGAACTCTGTAAATA+TGG	-	chr8_1:67680090-67680109	None:intergenic	20.0%
!!	TTTATTGAATTGTGAAACAT+AGG	-	chr8_1:67680148-67680167	None:intergenic	20.0%
!!	AAAAAGAGAATTTACATTCT+AGG	-	chr8_1:67680540-67680559	None:intergenic	20.0%
!!	TTGAAAGAGTTAAAGAATTA+AGG	+	chr8_1:67680644-67680663	Msa1209240:intron	20.0%
!!!	AAGACATGGTATTTTTTTAT+AGG	-	chr8_1:67680726-67680745	None:intergenic	20.0%
!!	TGTATAATTTGGGTAAATAT+GGG	-	chr8_1:67680958-67680977	None:intergenic	20.0%
!!!	CATTTAATTTCATCTTATCT+AGG	+	chr8_1:67681017-67681036	Msa1209240:intron	20.0%
!!!	TAAATTGATAGTTGTTTGTT+TGG	-	chr8_1:67681717-67681736	None:intergenic	20.0%
!!	TAAAATTATTCATGAATCCA+TGG	-	chr8_1:67681875-67681894	None:intergenic	20.0%
!!!	AATTGATGGTGTTTATAATT+TGG	+	chr8_1:67682481-67682500	Msa1209240:three_prime_UTR	20.0%
!!	AAACTTAGACTCATTAAAAA+TGG	-	chr8_1:67682560-67682579	None:intergenic	20.0%
!	TGAAATCCTAAGTTACTAAT+CGG	-	chr8_1:67678764-67678783	None:intergenic	25.0%
!	TAAAGAAAATAATAGACACC+TGG	-	chr8_1:67679319-67679338	None:intergenic	25.0%
!!!	GAAAAGGAGTATATTTTGAA+AGG	-	chr8_1:67679377-67679396	None:intergenic	25.0%
!	TACAAAAAAAGAAACATGCT+TGG	+	chr8_1:67679482-67679501	Msa1209240:intron	25.0%
!	ACAAAAAAAGAAACATGCTT+GGG	+	chr8_1:67679483-67679502	Msa1209240:intron	25.0%
!	ACGCTTTATAATCTATTGTT+AGG	-	chr8_1:67679518-67679537	None:intergenic	25.0%
!	TTGAGTGTACAAAAAATACA+AGG	+	chr8_1:67679621-67679640	Msa1209240:intron	25.0%
!!!	AATACTTTTGAAAACTACAC+CGG	+	chr8_1:67679762-67679781	Msa1209240:intron	25.0%
!	CACATCAATTTGAATATTGT+GGG	-	chr8_1:67679970-67679989	None:intergenic	25.0%
!	TCACATCAATTTGAATATTG+TGG	-	chr8_1:67679971-67679990	None:intergenic	25.0%
!	AATGAGTAAAAGCTATAGAT+TGG	-	chr8_1:67680037-67680056	None:intergenic	25.0%
!	TTTACAGAGTTCAAATTTGT+TGG	+	chr8_1:67680092-67680111	Msa1209240:intron	25.0%
!	AATTAGAGATGAAATATGCA+TGG	-	chr8_1:67680269-67680288	None:intergenic	25.0%
!!!	GAACATTTTAAAAAAGAGTG+TGG	-	chr8_1:67680414-67680433	None:intergenic	25.0%
!	ATAGCTTAAATCAAAGAAAG+AGG	-	chr8_1:67680497-67680516	None:intergenic	25.0%
!!	ACATTTCATTTCTAAAGACA+TGG	-	chr8_1:67680740-67680759	None:intergenic	25.0%
!	TGTTGAATATCATTAGAATC+TGG	+	chr8_1:67680757-67680776	Msa1209240:intron	25.0%
!!	AAAATTGAACATTTTGTGAG+AGG	-	chr8_1:67680828-67680847	None:intergenic	25.0%
!	AAAAGGTTCATCTTCTAATT+TGG	+	chr8_1:67680865-67680884	Msa1209240:intron	25.0%
!	GTGTATAATTTGGGTAAATA+TGG	-	chr8_1:67680959-67680978	None:intergenic	25.0%
!	AAGTTTGTGGTGTATAATTT+GGG	-	chr8_1:67680968-67680987	None:intergenic	25.0%
!!!	TTTTTTCATGTACAAGTTTG+TGG	-	chr8_1:67680981-67681000	None:intergenic	25.0%
!	TTTATAGTGTTATGCTTCAA+AGG	+	chr8_1:67681235-67681254	Msa1209240:intron	25.0%
!!	AATTTCCAAAGGTTTAAAAC+AGG	-	chr8_1:67681358-67681377	None:intergenic	25.0%
!	TGTTGAATTTGAATTTCCAA+AGG	-	chr8_1:67681369-67681388	None:intergenic	25.0%
!!	TACTTGATTTTCTATATGAC+AGG	+	chr8_1:67681398-67681417	Msa1209240:intron	25.0%
!!	ACTTGATTTTCTATATGACA+GGG	+	chr8_1:67681399-67681418	Msa1209240:intron	25.0%
!!!	TTTTCCTTTGTTTTTTTACC+TGG	+	chr8_1:67681532-67681551	Msa1209240:intron	25.0%
!!!	AACATTTTTTATCATGTGGA+AGG	-	chr8_1:67681567-67681586	None:intergenic	25.0%
!!!	ACTCAACATTTTTTATCATG+TGG	-	chr8_1:67681571-67681590	None:intergenic	25.0%
!!!	CAGATCAAAGCATTTTATAA+GGG	-	chr8_1:67681612-67681631	None:intergenic	25.0%
!!!	ACAGATCAAAGCATTTTATA+AGG	-	chr8_1:67681613-67681632	None:intergenic	25.0%
!!	TTTTCAAATGAGATCTTCAA+CGG	-	chr8_1:67681642-67681661	None:intergenic	25.0%
!	TTTAGCATTGTCATAAAATG+AGG	+	chr8_1:67681891-67681910	Msa1209240:intron	25.0%
!	CATAAAATGAGGTAATATCA+TGG	+	chr8_1:67681902-67681921	Msa1209240:intron	25.0%
!	TTTCTACTCATAAAGAGATA+GGG	-	chr8_1:67681949-67681968	None:intergenic	25.0%
!!	AAATATGATGGAAACACAAT+CGG	-	chr8_1:67682051-67682070	None:intergenic	25.0%
!	TGGTTTAATGTCAAATATGA+TGG	-	chr8_1:67682063-67682082	None:intergenic	25.0%
!!	ATGTATTCTCATAATGGTTT+TGG	-	chr8_1:67682307-67682326	None:intergenic	25.0%
!	CAGAAAATGTATTCTCATAA+TGG	-	chr8_1:67682313-67682332	None:intergenic	25.0%
!!	CATTATGAGAATACATTTTC+TGG	+	chr8_1:67682311-67682330	Msa1209240:three_prime_UTR	25.0%
!	TTGAAAAGTGAATCAATTGA+TGG	+	chr8_1:67682467-67682486	Msa1209240:three_prime_UTR	25.0%
!!	TGCATTTTGCTATACTTCTT+TGG	-	chr8_1:67678887-67678906	None:intergenic	30.0%
	CAATAGCAATAGCAATATCA+TGG	+	chr8_1:67678988-67679007	Msa1209240:CDS	30.0%
	GCAATTGTAGAATCTCAAAT+TGG	+	chr8_1:67679152-67679171	Msa1209240:intron	30.0%
	TGTAGAATCTCAAATTGGAA+TGG	+	chr8_1:67679157-67679176	Msa1209240:intron	30.0%
!	TAAGAACTGAGCATTGTTAT+AGG	+	chr8_1:67679184-67679203	Msa1209240:intron	30.0%
	AGCATTGTTATAGGATTACA+AGG	+	chr8_1:67679193-67679212	Msa1209240:intron	30.0%
!	ACTCACATGTTACTTAGAAA+AGG	-	chr8_1:67679393-67679412	None:intergenic	30.0%
!!	GGTGTAGTTTTCAAAAGTAT+TGG	-	chr8_1:67679763-67679782	None:intergenic	30.0%
	TCTTCTACAAAGAGTTCAAA+GGG	-	chr8_1:67679821-67679840	None:intergenic	30.0%
	ATCTTCTACAAAGAGTTCAA+AGG	-	chr8_1:67679822-67679841	None:intergenic	30.0%
	CAATTTGAATATTGTGGGAT+AGG	-	chr8_1:67679965-67679984	None:intergenic	30.0%
	TCTTCTCAATAAGTTAGACA+GGG	+	chr8_1:67680058-67680077	Msa1209240:intron	30.0%
!!	AGTTCAAATTTGTTGGAGTA+TGG	+	chr8_1:67680099-67680118	Msa1209240:intron	30.0%
	TAAGGTTCAAATTCTGATGA+AGG	+	chr8_1:67680662-67680681	Msa1209240:intron	30.0%
!	CTTTTCTTCTCTAGTAGAAA+AGG	+	chr8_1:67680848-67680867	Msa1209240:intron	30.0%
	GTTGTGATCTGTAATGAAAA+TGG	+	chr8_1:67680891-67680910	Msa1209240:intron	30.0%
	CAAGTTTGTGGTGTATAATT+TGG	-	chr8_1:67680969-67680988	None:intergenic	30.0%
!	ATTTTCCCTTTTAAATCCAC+AGG	+	chr8_1:67681266-67681285	Msa1209240:intron	30.0%
!	TTTTCCCTTTTAAATCCACA+GGG	+	chr8_1:67681267-67681286	Msa1209240:intron	30.0%
!!	AAAGACCTGTTTTAAACCTT+TGG	+	chr8_1:67681350-67681369	Msa1209240:intron	30.0%
!	CTTGATTTTCTATATGACAG+GGG	+	chr8_1:67681400-67681419	Msa1209240:intron	30.0%
!	TTGATTTTCTATATGACAGG+GGG	+	chr8_1:67681401-67681420	Msa1209240:intron	30.0%
!!	TACCATTTGGTGTGTTTAAT+GGG	-	chr8_1:67681492-67681511	None:intergenic	30.0%
	ATAGCCAGGTAAAAAAACAA+AGG	-	chr8_1:67681539-67681558	None:intergenic	30.0%
	AATGTTGAGTGTCTAGATTA+TGG	+	chr8_1:67681581-67681600	Msa1209240:intron	30.0%
	TTTCAAATGAGATCTTCAAC+GGG	-	chr8_1:67681641-67681660	None:intergenic	30.0%
	TCATGAATCCATGGAAAATA+GGG	-	chr8_1:67681866-67681885	None:intergenic	30.0%
	TTCATGAATCCATGGAAAAT+AGG	-	chr8_1:67681867-67681886	None:intergenic	30.0%
	GTTTCTACTCATAAAGAGAT+AGG	-	chr8_1:67681950-67681969	None:intergenic	30.0%
	ATCTCTTTATGAGTAGAAAC+TGG	+	chr8_1:67681950-67681969	Msa1209240:intron	30.0%
	AACGATCAAAATGCAATCAT+TGG	-	chr8_1:67682001-67682020	None:intergenic	30.0%
!!	TGATCGTTTTGTTCAAAGAT+TGG	+	chr8_1:67682013-67682032	Msa1209240:intron	30.0%
!!	TTGTTCAAAGATTGGTTTGA+AGG	+	chr8_1:67682021-67682040	Msa1209240:intron	30.0%
!	ATGATCCATGTTGATAAAGT+AGG	+	chr8_1:67682119-67682138	Msa1209240:CDS	30.0%
	ATACTCCATGTGCTAATAAA+TGG	-	chr8_1:67682255-67682274	None:intergenic	30.0%
	ATTTATTAGCACATGGAGTA+TGG	+	chr8_1:67682254-67682273	Msa1209240:three_prime_UTR	30.0%
	TTTATTAGCACATGGAGTAT+GGG	+	chr8_1:67682255-67682274	Msa1209240:three_prime_UTR	30.0%
	AATGCTATGTGCTTATAGTT+TGG	-	chr8_1:67682588-67682607	None:intergenic	30.0%
	TTTGAACATTAGCACTGTTA+AGG	+	chr8_1:67682606-67682625	Msa1209240:three_prime_UTR	30.0%
	AAGTAAAACGAACATAACGT+GGG	-	chr8_1:67682666-67682685	None:intergenic	30.0%
	AAAGTAAAACGAACATAACG+TGG	-	chr8_1:67682667-67682686	None:intergenic	30.0%
!	GGTATGTATGGTTTGGAATT+GGG	-	chr8_1:67678812-67678831	None:intergenic	35.0%
	GTTAAGTAGTACCAAGAGTT+TGG	+	chr8_1:67678916-67678935	Msa1209240:CDS	35.0%
	TAGCAATAGCAATATCATGG+TGG	+	chr8_1:67678991-67679010	Msa1209240:CDS	35.0%
	GGTGGCATGTAGTAATAATT+CGG	+	chr8_1:67679012-67679031	Msa1209240:CDS	35.0%
	GATAGAAATGAGGTTGAACT+TGG	-	chr8_1:67679052-67679071	None:intergenic	35.0%
	GTTCAACCTCATTTCTATCT+CGG	+	chr8_1:67679053-67679072	Msa1209240:CDS	35.0%
!!	TTGTTGTTTCAACAGAGCAT+AGG	-	chr8_1:67679086-67679105	None:intergenic	35.0%
	TGCTCTGTTGAAACAACAAA+TGG	+	chr8_1:67679087-67679106	Msa1209240:CDS	35.0%
!	GCTTAAGTTGTTTGAAGAAG+AGG	+	chr8_1:67679238-67679257	Msa1209240:CDS	35.0%
	TTCTAAGTAACATGTGAGTG+AGG	+	chr8_1:67679394-67679413	Msa1209240:intron	35.0%
	TACACTCAAGAGCTAATTGT+TGG	-	chr8_1:67679610-67679629	None:intergenic	35.0%
	GTATCAAAGTTACACGAGAA+TGG	+	chr8_1:67679688-67679707	Msa1209240:intron	35.0%
	TGTAGAAGATTCACAACCTT+TGG	+	chr8_1:67679832-67679851	Msa1209240:intron	35.0%
!	CTAGTTGTGTTTGACACTTT+TGG	-	chr8_1:67679868-67679887	None:intergenic	35.0%
!!	TTTGCTTAAAGCGGTTTTGA+TGG	+	chr8_1:67679936-67679955	Msa1209240:intron	35.0%
	CTCTTCTCAATAAGTTAGAC+AGG	+	chr8_1:67680057-67680076	Msa1209240:intron	35.0%
	ATAGGATCACATAGACTGAT+AGG	-	chr8_1:67680130-67680149	None:intergenic	35.0%
	TATGTCGATCTGATAGAATG+TGG	-	chr8_1:67680246-67680265	None:intergenic	35.0%
	GTATGATCACTTCTTTCACT+TGG	+	chr8_1:67680453-67680472	Msa1209240:intron	35.0%
!!	CAAAGAAAGAGGCATCTATA+AGG	-	chr8_1:67680486-67680505	None:intergenic	35.0%
!	ACCTAGGGAGTATATATATG+AGG	-	chr8_1:67680795-67680814	None:intergenic	35.0%
!	AGTCAGGAGTGTATATATAG+AGG	+	chr8_1:67681053-67681072	Msa1209240:CDS	35.0%
!	GTCAGGAGTGTATATATAGA+GGG	+	chr8_1:67681054-67681073	Msa1209240:CDS	35.0%
	CAGGTCTTTATTAACATAGC+TGG	-	chr8_1:67681339-67681358	None:intergenic	35.0%
!	GAGTACTCAAAACTAGTTCT+GGG	-	chr8_1:67681460-67681479	None:intergenic	35.0%
!!	CTACCATTTGGTGTGTTTAA+TGG	-	chr8_1:67681493-67681512	None:intergenic	35.0%
	AAACACACCAAATGGTAGAA+TGG	+	chr8_1:67681495-67681514	Msa1209240:CDS	35.0%
	GTTCTTACCATTCTACCATT+TGG	-	chr8_1:67681505-67681524	None:intergenic	35.0%
	CGTCAAAGCATGTAAAACAT+GGG	-	chr8_1:67681831-67681850	None:intergenic	35.0%
	ACGTCAAAGCATGTAAAACA+TGG	-	chr8_1:67681832-67681851	None:intergenic	35.0%
	TGCAATATGATACAAGGTTC+TGG	-	chr8_1:67682083-67682102	None:intergenic	35.0%
	AGAACCTTGTATCATATTGC+AGG	+	chr8_1:67682082-67682101	Msa1209240:intron	35.0%
	TGTGTCCATTTATTAGCACA+TGG	+	chr8_1:67682247-67682266	Msa1209240:three_prime_UTR	35.0%
!	AATTTGGAACAACCATGTAG+AGG	+	chr8_1:67682497-67682516	Msa1209240:three_prime_UTR	35.0%
	ACATTAGCACTGTTAAGGTA+CGG	+	chr8_1:67682611-67682630	Msa1209240:three_prime_UTR	35.0%
	ACGACAAGGACTAAGTCTAA+GGG	+	chr8_1:67678738-67678757	Msa1209240:five_prime_UTR	40.0%
	TAAGGGCCGATTAGTAACTT+AGG	+	chr8_1:67678755-67678774	Msa1209240:five_prime_UTR	40.0%
!	GTTTGGAATTGGGTGGTTAA+TGG	-	chr8_1:67678802-67678821	None:intergenic	40.0%
!	ATGTATGGTTTGGAATTGGG+TGG	-	chr8_1:67678809-67678828	None:intergenic	40.0%
!	GGGTATGTATGGTTTGGAAT+TGG	-	chr8_1:67678813-67678832	None:intergenic	40.0%
	AAGTAGTACCAAGAGTTTGG+CGG	+	chr8_1:67678919-67678938	Msa1209240:CDS	40.0%
	CAATAGCAATATCATGGTGG+TGG	+	chr8_1:67678994-67679013	Msa1209240:CDS	40.0%
	TTGAGTCCGAGATAGAAATG+AGG	-	chr8_1:67679062-67679081	None:intergenic	40.0%
!	AGGAACCTGTTTTGCGTTTA+AGG	+	chr8_1:67679258-67679277	Msa1209240:CDS	40.0%
!	AACCTGTTTTGCGTTTAAGG+AGG	+	chr8_1:67679261-67679280	Msa1209240:CDS	40.0%
	GCGTTTAAGGAGGCTAATTA+AGG	+	chr8_1:67679271-67679290	Msa1209240:CDS	40.0%
	AATTGCTGATGAGAGATTCC+AGG	+	chr8_1:67679298-67679317	Msa1209240:CDS	40.0%
	TAACTCTCACTCAGATGGTA+TGG	+	chr8_1:67679429-67679448	Msa1209240:intron	40.0%
	ACTCAAGAGCTAATTGTTGG+AGG	-	chr8_1:67679607-67679626	None:intergenic	40.0%
	CAAAGTTACACGAGAATGGA+AGG	+	chr8_1:67679692-67679711	Msa1209240:intron	40.0%
!!!	TTGGTTTTGCCATCCAGAAA+GGG	-	chr8_1:67679744-67679763	None:intergenic	40.0%
!!!	ATTGGTTTTGCCATCCAGAA+AGG	-	chr8_1:67679745-67679764	None:intergenic	40.0%
!	TTTGGCCACTTTGCTTAAAG+CGG	+	chr8_1:67679927-67679946	Msa1209240:intron	40.0%
!	CAAAACCGCTTTAAGCAAAG+TGG	-	chr8_1:67679935-67679954	None:intergenic	40.0%
!	CACTCTTCCCTCTTATTTCT+AGG	+	chr8_1:67680350-67680369	Msa1209240:intron	40.0%
	GCTGCATCCTAGAAATAAGA+GGG	-	chr8_1:67680360-67680379	None:intergenic	40.0%
	AGCTGCATCCTAGAAATAAG+AGG	-	chr8_1:67680361-67680380	None:intergenic	40.0%
!	GCTCAACTAGTTTGAGCTAA+AGG	+	chr8_1:67680612-67680631	Msa1209240:intron	40.0%
	GCCTCATATATATACTCCCT+AGG	+	chr8_1:67680791-67680810	Msa1209240:intron	40.0%
!!	AGAGGCTATTCTGAAACCTA+GGG	-	chr8_1:67680810-67680829	None:intergenic	40.0%
	TTCATCTTATCTAGGGAGTC+AGG	+	chr8_1:67681025-67681044	Msa1209240:intron	40.0%
	TCATCTTATCTAGGGAGTCA+GGG	+	chr8_1:67681026-67681045	Msa1209240:intron	40.0%
!	AAAAAGTATAGCCCCTTCGA+AGG	-	chr8_1:67681090-67681109	None:intergenic	40.0%
!!	AACGGTGTCATCTGAGTTAT+TGG	-	chr8_1:67681133-67681152	None:intergenic	40.0%
	ACACCGTTCAACTTCTTAGA+AGG	+	chr8_1:67681145-67681164	Msa1209240:CDS	40.0%
!	GTGCCTTCTAAGAAGTTGAA+CGG	-	chr8_1:67681151-67681170	None:intergenic	40.0%
!	TTCAACTTCTTAGAAGGCAC+TGG	+	chr8_1:67681151-67681170	Msa1209240:CDS	40.0%
	TGGATCATAACCGATGCTAA+CGG	+	chr8_1:67681171-67681190	Msa1209240:CDS	40.0%
	ACGGGAGATCAGAAAATGTA+CGG	+	chr8_1:67681190-67681209	Msa1209240:CDS	40.0%
	AGTGCCCTGTGGATTTAAAA+GGG	-	chr8_1:67681274-67681293	None:intergenic	40.0%
	AAGTGCCCTGTGGATTTAAA+AGG	-	chr8_1:67681275-67681294	None:intergenic	40.0%
!!	TTTTAAATCCACAGGGCACT+TGG	+	chr8_1:67681274-67681293	Msa1209240:intron	40.0%
	TTTCTATATGACAGGGGGAT+TGG	+	chr8_1:67681406-67681425	Msa1209240:intron	40.0%
	TTCTATATGACAGGGGGATT+GGG	+	chr8_1:67681407-67681426	Msa1209240:intron	40.0%
!	GGAGTACTCAAAACTAGTTC+TGG	-	chr8_1:67681461-67681480	None:intergenic	40.0%
	TGCCCATTAAACACACCAAA+TGG	+	chr8_1:67681487-67681506	Msa1209240:CDS	40.0%
	TGTGGAAGGAAATTATAGCC+AGG	-	chr8_1:67681553-67681572	None:intergenic	40.0%
	GTTGAGTGTCTAGATTATGG+TGG	+	chr8_1:67681584-67681603	Msa1209240:intron	40.0%
!	TGATTGCACCCTATTTTCCA+TGG	+	chr8_1:67681855-67681874	Msa1209240:intron	40.0%
	AGAGATAGGGAAAGTTGTTG+CGG	-	chr8_1:67681936-67681955	None:intergenic	40.0%
	CCTTCCTGCAATATGATACA+AGG	-	chr8_1:67682089-67682108	None:intergenic	40.0%
	CCTTGTATCATATTGCAGGA+AGG	+	chr8_1:67682086-67682105	Msa1209240:intron	40.0%
	GTGAGCCTACTTTATCAACA+TGG	-	chr8_1:67682127-67682146	None:intergenic	40.0%
	GCAATGATCGCACTAAATGA+AGG	-	chr8_1:67682149-67682168	None:intergenic	40.0%
	TGCTGCAGTTCAATCTCAAA+TGG	-	chr8_1:67682213-67682232	None:intergenic	40.0%
	TAAATGGACACAACCTCTGA+AGG	-	chr8_1:67682239-67682258	None:intergenic	40.0%
	AGACTTAGTCCTTGTCGTGA+GGG	-	chr8_1:67678736-67678755	None:intergenic	45.0%
	TAGACTTAGTCCTTGTCGTG+AGG	-	chr8_1:67678737-67678756	None:intergenic	45.0%
	CACGACAAGGACTAAGTCTA+AGG	+	chr8_1:67678737-67678756	Msa1209240:five_prime_UTR	45.0%
	AGTAGTACCAAGAGTTTGGC+GGG	+	chr8_1:67678920-67678939	Msa1209240:CDS	45.0%
	GGAAGTAGCTGCTAAATCTG+AGG	+	chr8_1:67679108-67679127	Msa1209240:CDS	45.0%
	AGCCTCCTTAAACGCAAAAC+AGG	-	chr8_1:67679266-67679285	None:intergenic	45.0%
	CTGAGTGAGAGTTATGCTCA+CGG	-	chr8_1:67679423-67679442	None:intergenic	45.0%
	GAGCATAACTCTCACTCAGA+TGG	+	chr8_1:67679424-67679443	Msa1209240:intron	45.0%
!	AGTGTGCCATTAACCCTTTC+TGG	+	chr8_1:67679728-67679747	Msa1209240:intron	45.0%
	TGCCATTAACCCTTTCTGGA+TGG	+	chr8_1:67679732-67679751	Msa1209240:intron	45.0%
	TGCCATCCAGAAAGGGTTAA+TGG	-	chr8_1:67679737-67679756	None:intergenic	45.0%
	AAAACTACACCGGTAGACCT+AGG	+	chr8_1:67679772-67679791	Msa1209240:intron	45.0%
	ATAGCAATGTTGCGTCTCCT+AGG	-	chr8_1:67679792-67679811	None:intergenic	45.0%
!!	GATTCACAACCTTTGGTGCT+AGG	+	chr8_1:67679839-67679858	Msa1209240:intron	45.0%
!!!	TTTTGGCTTCCTAGCACCAA+AGG	-	chr8_1:67679851-67679870	None:intergenic	45.0%
	TGAACATGCTTTCTCGCGTT+TGG	+	chr8_1:67679909-67679928	Msa1209240:intron	45.0%
	TTCCAGTGATGCTACAACGT+TGG	+	chr8_1:67680431-67680450	Msa1209240:CDS	45.0%
	TACCAACGTTGTAGCATCAC+TGG	-	chr8_1:67680436-67680455	None:intergenic	45.0%
!!	GAGAGGCTATTCTGAAACCT+AGG	-	chr8_1:67680811-67680830	None:intergenic	45.0%
	GGATCATAACCGATGCTAAC+GGG	+	chr8_1:67681172-67681191	Msa1209240:CDS	45.0%
	TTCTGATCTCCCGTTAGCAT+CGG	-	chr8_1:67681184-67681203	None:intergenic	45.0%
	TCTATATGACAGGGGGATTG+GGG	+	chr8_1:67681408-67681427	Msa1209240:intron	45.0%
!!	CTAGTTCTGGGAAGAAGTAC+TGG	-	chr8_1:67681448-67681467	None:intergenic	45.0%
!	TGTGTTTAATGGGCATGCAG+AGG	-	chr8_1:67681482-67681501	None:intergenic	45.0%
	AGATAGGGAAAGTTGTTGCG+GGG	-	chr8_1:67681934-67681953	None:intergenic	45.0%
	GAGATAGGGAAAGTTGTTGC+GGG	-	chr8_1:67681935-67681954	None:intergenic	45.0%
	AAGCAGAGCGAGAGAAAATG+GGG	-	chr8_1:67682171-67682190	None:intergenic	45.0%
	CAAGCAGAGCGAGAGAAAAT+GGG	-	chr8_1:67682172-67682191	None:intergenic	45.0%
	TAGCACATGGAGTATGGGAA+AGG	+	chr8_1:67682260-67682279	Msa1209240:three_prime_UTR	45.0%
	ACATGGAGTATGGGAAAGGA+AGG	+	chr8_1:67682264-67682283	Msa1209240:three_prime_UTR	45.0%
	GGAAGGATTATCGTTCTCAC+TGG	+	chr8_1:67682281-67682300	Msa1209240:three_prime_UTR	45.0%
!	CTTTTTGTGTACGCAGAGGT+TGG	-	chr8_1:67682438-67682457	None:intergenic	45.0%
	ACCTCTGCGTACACAAAAAG+AGG	+	chr8_1:67682438-67682457	Msa1209240:three_prime_UTR	45.0%
	CCTCTGCGTACACAAAAAGA+GGG	+	chr8_1:67682439-67682458	Msa1209240:three_prime_UTR	45.0%
	GAACAACCATGTAGAGGAAG+TGG	+	chr8_1:67682503-67682522	Msa1209240:three_prime_UTR	45.0%
	CAACATCCACTTCCTCTACA+TGG	-	chr8_1:67682512-67682531	None:intergenic	45.0%
	CATGTAGAGGAAGTGGATGT+TGG	+	chr8_1:67682510-67682529	Msa1209240:three_prime_UTR	45.0%
!!	CCTTGTCGTGAGGGTATTCA+CGG	-	chr8_1:67678727-67678746	None:intergenic	50.0%
	GTTCGCGGGTATGTATGGTT+TGG	-	chr8_1:67678819-67678838	None:intergenic	50.0%
	AACCATACATACCCGCGAAC+AGG	+	chr8_1:67678819-67678838	Msa1209240:five_prime_UTR	50.0%
	ATCCTGTTCGCGGGTATGTA+TGG	-	chr8_1:67678824-67678843	None:intergenic	50.0%
	CAGGATCAGTGAGGACGATA+AGG	+	chr8_1:67678838-67678857	Msa1209240:five_prime_UTR	50.0%
	ACACGAGAATGGAAGGCAAG+CGG	+	chr8_1:67679699-67679718	Msa1209240:intron	50.0%
	GGAAGAAGTCAGCCTTCGAA+GGG	+	chr8_1:67681075-67681094	Msa1209240:CDS	50.0%
	GAAGAAGTCAGCCTTCGAAG+GGG	+	chr8_1:67681076-67681095	Msa1209240:CDS	50.0%
	GACATTAACCAAGTGCCCTG+TGG	-	chr8_1:67681285-67681304	None:intergenic	50.0%
	CCAAGCAGAGCGAGAGAAAA+TGG	-	chr8_1:67682173-67682192	None:intergenic	50.0%
!	CCATTTTCTCTCGCTCTGCT+TGG	+	chr8_1:67682170-67682189	Msa1209240:CDS	50.0%
	AACTGCAGCATCACCTTCAG+AGG	+	chr8_1:67682223-67682242	Msa1209240:three_prime_UTR	50.0%
!	CCCTCTTTTTGTGTACGCAG+AGG	-	chr8_1:67682442-67682461	None:intergenic	50.0%
	CCGTGAATACCCTCACGACA+AGG	+	chr8_1:67678724-67678743	Msa1209240:five_prime_UTR	55.0%
	TCCTCACTGATCCTGTTCGC+GGG	-	chr8_1:67678833-67678852	None:intergenic	55.0%
	GTCCTCACTGATCCTGTTCG+CGG	-	chr8_1:67678834-67678853	None:intergenic	55.0%
!!	CCAAGAGTTTGGCGGGTTTG+CGG	+	chr8_1:67678927-67678946	Msa1209240:CDS	55.0%
!!	TTTGGCGGGTTTGCGGTTGA+TGG	+	chr8_1:67678934-67678953	Msa1209240:CDS	55.0%
!!	TTGGCGGGTTTGCGGTTGAT+GGG	+	chr8_1:67678935-67678954	Msa1209240:CDS	55.0%
!!	GGTTGATGGGTCAAGAAGCG+AGG	+	chr8_1:67678948-67678967	Msa1209240:CDS	55.0%
	GTCAAGAAGCGAGGCTGAAG+AGG	+	chr8_1:67678957-67678976	Msa1209240:CDS	55.0%
	AAGAAGCGAGGCTGAAGAGG+AGG	+	chr8_1:67678960-67678979	Msa1209240:CDS	55.0%
	AGAAGCGAGGCTGAAGAGGA+GGG	+	chr8_1:67678961-67678980	Msa1209240:CDS	55.0%
	CACGAGAATGGAAGGCAAGC+GGG	+	chr8_1:67679700-67679719	Msa1209240:intron	55.0%
	GTTGCGTCTCCTAGGTCTAC+CGG	-	chr8_1:67679784-67679803	None:intergenic	55.0%
	AGGGAGTCAGGGTTCAAGTC+AGG	+	chr8_1:67681037-67681056	Msa1209240:intron	55.0%
	GGGAAGAAGTCAGCCTTCGA+AGG	+	chr8_1:67681074-67681093	Msa1209240:CDS	55.0%
!!	GTTGGTTTGCCTTGCGCTCA+CGG	+	chr8_1:67682528-67682547	Msa1209240:three_prime_UTR	55.0%
	ACCCGCGAACAGGATCAGTG+AGG	+	chr8_1:67678829-67678848	Msa1209240:five_prime_UTR	60.0%
	CCGCAAACCCGCCAAACTCT+TGG	-	chr8_1:67678930-67678949	None:intergenic	60.0%
	GAAGCGAGGCTGAAGAGGAG+GGG	+	chr8_1:67678962-67678981	Msa1209240:CDS	60.0%
	GACAGGGGGATTGGGGTTGT+TGG	+	chr8_1:67681415-67681434	Msa1209240:intron	60.0%
	TGGCTACAACCGTGAGCGCA+AGG	-	chr8_1:67682540-67682559	None:intergenic	60.0%
	GCGAGGCTGAAGAGGAGGGG+TGG	+	chr8_1:67678965-67678984	Msa1209240:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	67678716	67682707	67678716	ID=Msa1209240;Name=Msa1209240
chr8_1	mRNA	67678716	67682707	67678716	ID=Msa1209240-mRNA-1;Parent=Msa1209240;Name=Msa1209240-mRNA-1;_AED=0.06;_eAED=0.06;_QI=186\|1\|1\|1\|1\|1\|6\|490\|268
chr8_1	exon	67678716	67679129	67678716	ID=Msa1209240-mRNA-1:exon:22363;Parent=Msa1209240-mRNA-1
chr8_1	exon	67679206	67679319	67679206	ID=Msa1209240-mRNA-1:exon:22364;Parent=Msa1209240-mRNA-1
chr8_1	exon	67680372	67680452	67680372	ID=Msa1209240-mRNA-1:exon:22365;Parent=Msa1209240-mRNA-1
chr8_1	exon	67681039	67681211	67681039	ID=Msa1209240-mRNA-1:exon:22366;Parent=Msa1209240-mRNA-1
chr8_1	exon	67681420	67681516	67681420	ID=Msa1209240-mRNA-1:exon:22367;Parent=Msa1209240-mRNA-1
chr8_1	exon	67682104	67682707	67682104	ID=Msa1209240-mRNA-1:exon:22368;Parent=Msa1209240-mRNA-1
chr8_1	five_prime_UTR	67678716	67678901	67678716	ID=Msa1209240-mRNA-1:five_prime_utr;Parent=Msa1209240-mRNA-1
chr8_1	CDS	67678902	67679129	67678902	ID=Msa1209240-mRNA-1:cds;Parent=Msa1209240-mRNA-1
chr8_1	CDS	67679206	67679319	67679206	ID=Msa1209240-mRNA-1:cds;Parent=Msa1209240-mRNA-1
chr8_1	CDS	67680372	67680452	67680372	ID=Msa1209240-mRNA-1:cds;Parent=Msa1209240-mRNA-1
chr8_1	CDS	67681039	67681211	67681039	ID=Msa1209240-mRNA-1:cds;Parent=Msa1209240-mRNA-1
chr8_1	CDS	67681420	67681516	67681420	ID=Msa1209240-mRNA-1:cds;Parent=Msa1209240-mRNA-1
chr8_1	CDS	67682104	67682217	67682104	ID=Msa1209240-mRNA-1:cds;Parent=Msa1209240-mRNA-1
chr8_1	three_prime_UTR	67682218	67682707	67682218	ID=Msa1209240-mRNA-1:three_prime_utr;Parent=Msa1209240-mRNA-1

Gene Sequence

>Msa1209240

ATGCAATCGTTATCGTTAAGTAGTACCAAGAGTTTGGCGGGTTTGCGGTTGATGGGTCAAGAAGCGAGGCTGAAGAGGAGGGGTGGCAATAGCAATAGCAATATCATGGTGGTGGCATGTAGTAATAATTCGGATAAAGACACTGCTCCAAGTTCAACCTCATTTCTATCTCGGACTCAAACCTATGCTCTGTTGAAACAACAAATGGAAGTAGCTGCTAAATCTGAGGATTACAAGGAAGCTGCTAGAATTCGAGACTCGCTTAAGTTGTTTGAAGAAGAGGAACCTGTTTTGCGTTTAAGGAGGCTAATTAAGGAAGCAATTGCTGATGAGAGATTCCAGGATGCAGCTAGTTATCGTGATGAGCTAAAAAGAATTGCTCCACACTCTTTTTTAAAATGTTCCAGTGATGCTACAACGTTGGGAGTCAGGGTTCAAGTCAGGAGTGTATATATAGAGGGAAGAAGTCAGCCTTCGAAGGGGCTATACTTTTTTGCATATAGAATAAGAATTACCAATAACTCAGATGACACCGTTCAACTTCTTAGAAGGCACTGGATCATAACCGATGCTAACGGGAGATCAGAAAATGTACGGGGGATTGGGGTTGTTGGTGAACAGCCAGTACTTCTTCCCAGAACTAGTTTTGAGTACTCCTCTGCATGCCCATTAAACACACCAAATGGTAGAATGGAAGGTGAATATGAGATGATCCATGTTGATAAAGTAGGCTCACCTTCATTTAGTGCGATCATTGCCCCATTTTCTCTCGCTCTGCTTGGAGATGATGACTGCAATACCATTTGA

Protein sequence

>Msa1209240

MQSLSLSSTKSLAGLRLMGQEARLKRRGGNSNSNIMVVACSNNSDKDTAPSSTSFLSRTQTYALLKQQMEVAAKSEDYKEAARIRDSLKLFEEEEPVLRLRRLIKEAIADERFQDAASYRDELKRIAPHSFLKCSSDATTLGVRVQVRSVYIEGRSQPSKGLYFFAYRIRITNNSDDTVQLLRRHWIITDANGRSENVRGIGVVGEQPVLLPRTSFEYSSACPLNTPNGRMEGEYEMIHVDKVGSPSFSAIIAPFSLALLGDDDCNTI*