AlfalfaGEDB | Msa1350350

M. sativa cultivar zhongmu-4 / Msa1350350

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1350350	XP_013452555.1	95.868	242	10	0	1	242	1	242	2.86e-166	469

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1350350	G7ZX37	95.868	242	10	0	1	242	1	242	1.37e-166	469

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa1350350	0.873358	1.564887e-67	-8.615850e-47
Msa0000610	Msa1350350	0.807759	4.321004e-50	-8.615850e-47
Msa0001040	Msa1350350	0.837068	6.463198e-57	-8.615850e-47
Msa0001510	Msa1350350	0.877971	4.093994e-69	-8.615850e-47
Msa0002110	Msa1350350	0.810267	1.254852e-50	-8.615850e-47
Msa0002690	Msa1350350	0.863769	1.964412e-64	-8.615850e-47
Msa0002700	Msa1350350	0.857609	1.452771e-62	-8.615850e-47
Msa0003340	Msa1350350	0.839690	1.362378e-57	-8.615850e-47
Msa0004180	Msa1350350	0.852332	4.948863e-61	-8.615850e-47
Msa0004340	Msa1350350	0.828434	9.010447e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1350350	MtrunA17_Chr6g0474081	95.868	242	10	0	1	242	1	242	2.63e-170	469

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1350350	AT5G62140.1	59.242	211	78	3	37	239	27	237	3.00e-80	241

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Find 63 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTCGGTATCCGATCCA+AGG	0.576342	tig0011376:-3683	None:intergenic
AAAGTGCAGACTGGTTGGTG+TGG	0.674945	tig0011376:+3371	Msa1350350:CDS
AAATGGGTCCTGCAAGTGGA+TGG	0.623567	tig0011376:+3632	Msa1350350:three_prime_UTR
AACACTGTCTTCACAAGTAG+TGG	0.578508	tig0011376:-3285	None:intergenic
ACCAGCAGAAAACAAGAACT+TGG	0.514821	tig0011376:-3244	None:intergenic
ACTGTCTTCACAAGTAGTGG+TGG	0.644274	tig0011376:-3282	None:intergenic
AGCTACAACAAAACCTTGTA+TGG	0.376682	tig0011376:-3607	None:intergenic
AGCTCTACAACAAAGTTTCT+TGG	0.268474	tig0011376:+1259	Msa1350350:CDS
AGTTCAGCAAGACACTCAGT+AGG	0.604330	tig0011376:-3444	None:intergenic
ATAAGAATATGCAAAATTGG+AGG	0.577452	tig0011376:+3750	Msa1350350:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGAAATTTGCATTGTATTGG+TGG	-	tig0011376:938-957	None:intergenic	30.0%
TGAAACTCCATCAAATGATA+AGG	+	tig0011376:1051-1070	Msa1350350:CDS	30.0%
GATACTTAAAAGATACTCGA+CGG	-	tig0011376:2410-2429	None:intergenic	30.0%
TGATCCTGTAATATTAGAAG+AGG	+	tig0011376:2715-2734	Msa1350350:intron	30.0%
GATCCTGTAATATTAGAAGA+GGG	+	tig0011376:2716-2735	Msa1350350:intron	30.0%
AAACCATTAACTATGAAGCA+CGG	-	tig0011376:3179-3198	None:intergenic	30.0%
TTCTGCTGGTAGTATATATA+AGG	+	tig0011376:3257-3276	Msa1350350:CDS	30.0%
TTGACATCTGAAGAATCAAA+GGG	+	tig0011376:3306-3325	Msa1350350:CDS	30.0%
CTCTTATGATGAAGAAGAAA+TGG	-	tig0011376:3484-3503	None:intergenic	30.0%
GTGTATGCCTTATCATTTGA+TGG	-	tig0011376:1061-1080	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0011376	gene	862	3993	862	ID=Msa1350350;Name=Msa1350350
tig0011376	mRNA	862	3993	862	ID=Msa1350350-mRNA-1;Parent=Msa1350350;Name=Msa1350350-mRNA-1;_AED=0.00;_eAED=0.00;_QI=46\|1\|1\|1\|1\|1\|2\|493\|242
tig0011376	exon	862	1360	862	ID=Msa1350350-mRNA-1:exon:7352;Parent=Msa1350350-mRNA-1
tig0011376	exon	3225	3993	3225	ID=Msa1350350-mRNA-1:exon:7353;Parent=Msa1350350-mRNA-1
tig0011376	five_prime_UTR	862	907	862	ID=Msa1350350-mRNA-1:five_prime_utr;Parent=Msa1350350-mRNA-1
tig0011376	CDS	908	1360	908	ID=Msa1350350-mRNA-1:cds;Parent=Msa1350350-mRNA-1
tig0011376	CDS	3225	3500	3225	ID=Msa1350350-mRNA-1:cds;Parent=Msa1350350-mRNA-1
tig0011376	three_prime_UTR	3501	3993	3501	ID=Msa1350350-mRNA-1:three_prime_utr;Parent=Msa1350350-mRNA-1

Gene Sequence

>Msa1350350

ATGAGTTCTAAGATAAATTTCTCAAGTCCACCAATACAATGCAAATTTCATATCAAGAAATCAAATATCACAAGAAAAAACATCATCATCTTCTCTTCAATTCCCTCAAGTGAAAAACAAAACAAAACAATTTCCAAAACAATTGAAACTCCATCAAATGATAAGGCATACACTATTAATTTCAAGACAAAAAGTGCTTGCAAGCTTGGAATTTCAAGATATCCGGATTTCGAATATGATGCTGAAGGAGGAATAGGAACTGGTTTTGGTGCAAAGGAAACACAAAATTCAGATAACAATGACATTCTTGTTTCATTTGATCTTGATACATTGTATATTCCATCATTAACAAGCTCTACAACAAAGTTTCTTGGTTTGTCATTGCCACCATTTTTGAAGATTGATATTGTTCCACAAGCTTTTCAAGGAAGTATCAATCAAGAATCTGGCAAGGTTGATCTTGAGTTTAAGGCCAAGTTCTTGTTTTCTGCTGGTAGTATATATAAGGCTCCACCACTACTTGTGAAGACAGTGTTGACATCTGAAGAATCAAAGGGGGCTATGAAGAGTGGAAGAGGCATGAGATTGGATGAAGAAGGAAAGTGCAGACTGGTTGGTGTGGCAACAGTTGATCCTATTGATGATTTCCTAATGAATTCCTTCCTTGGTCTACCTACTGAGTGTCTTGCTGAACTAAATGCTGTAATCTCCATTTCTTCTTCATCATAA

Protein sequence

>Msa1350350

MSSKINFSSPPIQCKFHIKKSNITRKNIIIFSSIPSSEKQNKTISKTIETPSNDKAYTINFKTKSACKLGISRYPDFEYDAEGGIGTGFGAKETQNSDNNDILVSFDLDTLYIPSLTSSTTKFLGLSLPPFLKIDIVPQAFQGSINQESGKVDLEFKAKFLFSAGSIYKAPPLLVKTVLTSEESKGAMKSGRGMRLDEEGKCRLVGVATVDPIDDFLMNSFLGLPTECLAELNAVISISSSS*