Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70120.t1 | XP_013446933.1 | 93.5 | 185 | 12 | 0 | 36 | 220 | 38 | 222 | 2.30E-102 | 381.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70120.t1 | Q67YC9 | 66.4 | 149 | 49 | 1 | 37 | 185 | 36 | 183 | 1.1e-57 | 224.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70120.t1 | A0A072U547 | 93.5 | 185 | 12 | 0 | 36 | 220 | 38 | 222 | 1.6e-102 | 381.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene70120 | 0.822529 | 2.26E-53 | -1.69E-46 |
| MS.gene049171 | MS.gene70120 | 0.868502 | 6.23E-66 | -1.69E-46 |
| MS.gene049173 | MS.gene70120 | 0.819702 | 1.01E-52 | -1.69E-46 |
| MS.gene049217 | MS.gene70120 | 0.873022 | 2.03E-67 | -1.69E-46 |
| MS.gene049222 | MS.gene70120 | 0.876188 | 1.70E-68 | -1.69E-46 |
| MS.gene049285 | MS.gene70120 | 0.905307 | 4.93E-80 | -1.69E-46 |
| MS.gene049320 | MS.gene70120 | 0.872919 | 2.20E-67 | -1.69E-46 |
| MS.gene049427 | MS.gene70120 | 0.848018 | 8.01E-60 | -1.69E-46 |
| MS.gene049609 | MS.gene70120 | 0.87475 | 5.29E-68 | -1.69E-46 |
| MS.gene049614 | MS.gene70120 | 0.807415 | 5.11E-50 | -1.69E-46 |
| MS.gene049615 | MS.gene70120 | 0.841536 | 4.48E-58 | -1.69E-46 |
| MS.gene049627 | MS.gene70120 | 0.815703 | 8.07E-52 | -1.69E-46 |
| MS.gene049633 | MS.gene70120 | 0.821343 | 4.25E-53 | -1.69E-46 |
| MS.gene049805 | MS.gene70120 | 0.866399 | 2.93E-65 | -1.69E-46 |
| MS.gene05010 | MS.gene70120 | 0.843742 | 1.16E-58 | -1.69E-46 |
| MS.gene050163 | MS.gene70120 | 0.877363 | 6.67E-69 | -1.69E-46 |
| MS.gene050165 | MS.gene70120 | 0.86914 | 3.87E-66 | -1.69E-46 |
| MS.gene050323 | MS.gene70120 | 0.804927 | 1.71E-49 | -1.69E-46 |
| MS.gene050333 | MS.gene70120 | 0.876991 | 8.99E-69 | -1.69E-46 |
| MS.gene050353 | MS.gene70120 | -0.808751 | 2.66E-50 | -1.69E-46 |
| MS.gene050432 | MS.gene70120 | 0.820213 | 7.72E-53 | -1.69E-46 |
| MS.gene050444 | MS.gene70120 | 0.803262 | 3.79E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene70120 | 0.864226 | 1.42E-64 | -1.69E-46 |
| MS.gene050518 | MS.gene70120 | 0.857813 | 1.26E-62 | -1.69E-46 |
| MS.gene050520 | MS.gene70120 | 0.804143 | 2.49E-49 | -1.69E-46 |
| MS.gene050538 | MS.gene70120 | 0.834081 | 3.68E-56 | -1.69E-46 |
| MS.gene050577 | MS.gene70120 | 0.808133 | 3.60E-50 | -1.69E-46 |
| MS.gene050593 | MS.gene70120 | 0.864148 | 1.50E-64 | -1.69E-46 |
| MS.gene050612 | MS.gene70120 | 0.876418 | 1.42E-68 | -1.69E-46 |
| MS.gene050675 | MS.gene70120 | 0.845237 | 4.60E-59 | -1.69E-46 |
| MS.gene050699 | MS.gene70120 | 0.855871 | 4.72E-62 | -1.69E-46 |
| MS.gene050747 | MS.gene70120 | 0.866408 | 2.91E-65 | -1.69E-46 |
| MS.gene050767 | MS.gene70120 | 0.861441 | 1.02E-63 | -1.69E-46 |
| MS.gene050770 | MS.gene70120 | 0.865763 | 4.66E-65 | -1.69E-46 |
| MS.gene050772 | MS.gene70120 | 0.893932 | 3.96E-75 | -1.69E-46 |
| MS.gene05080 | MS.gene70120 | 0.830143 | 3.47E-55 | -1.69E-46 |
| MS.gene050868 | MS.gene70120 | 0.828073 | 1.10E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene70120 | 0.825551 | 4.41E-54 | -1.69E-46 |
| MS.gene050950 | MS.gene70120 | 0.866707 | 2.34E-65 | -1.69E-46 |
| MS.gene050986 | MS.gene70120 | 0.849011 | 4.25E-60 | -1.69E-46 |
| MS.gene05100 | MS.gene70120 | 0.846811 | 1.72E-59 | -1.69E-46 |
| MS.gene05104 | MS.gene70120 | -0.806909 | 6.54E-50 | -1.69E-46 |
| MS.gene05105 | MS.gene70120 | 0.811788 | 5.87E-51 | -1.69E-46 |
| MS.gene051228 | MS.gene70120 | 0.866724 | 2.31E-65 | -1.69E-46 |
| MS.gene051317 | MS.gene70120 | 0.806831 | 6.80E-50 | -1.69E-46 |
| MS.gene051387 | MS.gene70120 | 0.840433 | 8.72E-58 | -1.69E-46 |
| MS.gene051434 | MS.gene70120 | 0.832362 | 9.87E-56 | -1.69E-46 |
| MS.gene051551 | MS.gene70120 | 0.816976 | 4.19E-52 | -1.69E-46 |
| MS.gene051617 | MS.gene70120 | 0.82579 | 3.87E-54 | -1.69E-46 |
| MS.gene051666 | MS.gene70120 | 0.861049 | 1.35E-63 | -1.69E-46 |
| MS.gene051694 | MS.gene70120 | 0.871831 | 5.06E-67 | -1.69E-46 |
| MS.gene051810 | MS.gene70120 | 0.878282 | 3.18E-69 | -1.69E-46 |
| MS.gene051838 | MS.gene70120 | 0.803154 | 3.99E-49 | -1.69E-46 |
| MS.gene051858 | MS.gene70120 | 0.855395 | 6.49E-62 | -1.69E-46 |
| MS.gene051949 | MS.gene70120 | 0.833672 | 4.66E-56 | -1.69E-46 |
| MS.gene052164 | MS.gene70120 | 0.82642 | 2.74E-54 | -1.69E-46 |
| MS.gene052271 | MS.gene70120 | 0.867894 | 9.77E-66 | -1.69E-46 |
| MS.gene052290 | MS.gene70120 | 0.804681 | 1.92E-49 | -1.69E-46 |
| MS.gene052322 | MS.gene70120 | 0.827774 | 1.30E-54 | -1.69E-46 |
| MS.gene052379 | MS.gene70120 | 0.881108 | 3.16E-70 | -1.69E-46 |
| MS.gene052421 | MS.gene70120 | 0.815023 | 1.14E-51 | -1.69E-46 |
| MS.gene052484 | MS.gene70120 | 0.846043 | 2.78E-59 | -1.69E-46 |
| MS.gene052486 | MS.gene70120 | 0.810468 | 1.14E-50 | -1.69E-46 |
| MS.gene052503 | MS.gene70120 | 0.803027 | 4.24E-49 | -1.69E-46 |
| MS.gene052506 | MS.gene70120 | 0.816931 | 4.29E-52 | -1.69E-46 |
| MS.gene052508 | MS.gene70120 | 0.80291 | 4.48E-49 | -1.69E-46 |
| MS.gene052510 | MS.gene70120 | 0.821782 | 3.36E-53 | -1.69E-46 |
| MS.gene052615 | MS.gene70120 | 0.843361 | 1.47E-58 | -1.69E-46 |
| MS.gene052805 | MS.gene70120 | -0.81008 | 1.38E-50 | -1.69E-46 |
| MS.gene052822 | MS.gene70120 | 0.830615 | 2.66E-55 | -1.69E-46 |
| MS.gene052870 | MS.gene70120 | 0.840593 | 7.92E-58 | -1.69E-46 |
| MS.gene052872 | MS.gene70120 | 0.84919 | 3.79E-60 | -1.69E-46 |
| MS.gene052916 | MS.gene70120 | 0.843793 | 1.13E-58 | -1.69E-46 |
| MS.gene052966 | MS.gene70120 | 0.835247 | 1.87E-56 | -1.69E-46 |
| MS.gene053009 | MS.gene70120 | 0.837988 | 3.76E-57 | -1.69E-46 |
| MS.gene053104 | MS.gene70120 | 0.850988 | 1.19E-60 | -1.69E-46 |
| MS.gene053107 | MS.gene70120 | 0.875392 | 3.20E-68 | -1.69E-46 |
| MS.gene053203 | MS.gene70120 | 0.854449 | 1.22E-61 | -1.69E-46 |
| MS.gene053207 | MS.gene70120 | 0.871551 | 6.27E-67 | -1.69E-46 |
| MS.gene053213 | MS.gene70120 | 0.893319 | 7.02E-75 | -1.69E-46 |
| MS.gene053286 | MS.gene70120 | 0.886478 | 3.30E-72 | -1.69E-46 |
| MS.gene053322 | MS.gene70120 | 0.88009 | 7.31E-70 | -1.69E-46 |
| MS.gene053436 | MS.gene70120 | 0.816538 | 5.25E-52 | -1.69E-46 |
| MS.gene053479 | MS.gene70120 | 0.800071 | 1.71E-48 | -1.69E-46 |
| MS.gene053561 | MS.gene70120 | -0.806589 | 7.64E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene70120 | 0.86691 | 2.02E-65 | -1.69E-46 |
| MS.gene053632 | MS.gene70120 | 0.849936 | 2.35E-60 | -1.69E-46 |
| MS.gene053824 | MS.gene70120 | 0.852004 | 6.13E-61 | -1.69E-46 |
| MS.gene053916 | MS.gene70120 | 0.846608 | 1.95E-59 | -1.69E-46 |
| MS.gene054376 | MS.gene70120 | 0.851194 | 1.04E-60 | -1.69E-46 |
| MS.gene054408 | MS.gene70120 | 0.832285 | 1.03E-55 | -1.69E-46 |
| MS.gene054595 | MS.gene70120 | 0.825715 | 4.03E-54 | -1.69E-46 |
| MS.gene054600 | MS.gene70120 | 0.874982 | 4.41E-68 | -1.69E-46 |
| MS.gene054642 | MS.gene70120 | 0.910295 | 2.20E-82 | -1.69E-46 |
| MS.gene054802 | MS.gene70120 | 0.842355 | 2.72E-58 | -1.69E-46 |
| MS.gene054803 | MS.gene70120 | 0.840171 | 1.02E-57 | -1.69E-46 |
| MS.gene054804 | MS.gene70120 | 0.84345 | 1.39E-58 | -1.69E-46 |
| MS.gene054927 | MS.gene70120 | 0.815404 | 9.40E-52 | -1.69E-46 |
| MS.gene054954 | MS.gene70120 | 0.870065 | 1.93E-66 | -1.69E-46 |
| MS.gene05505 | MS.gene70120 | 0.85794 | 1.16E-62 | -1.69E-46 |
| MS.gene055154 | MS.gene70120 | -0.806965 | 6.37E-50 | -1.69E-46 |
| MS.gene055206 | MS.gene70120 | 0.87588 | 2.17E-68 | -1.69E-46 |
| MS.gene055212 | MS.gene70120 | 0.838124 | 3.47E-57 | -1.69E-46 |
| MS.gene055224 | MS.gene70120 | 0.842341 | 2.74E-58 | -1.69E-46 |
| MS.gene055254 | MS.gene70120 | 0.807922 | 3.99E-50 | -1.69E-46 |
| MS.gene055289 | MS.gene70120 | 0.855729 | 5.19E-62 | -1.69E-46 |
| MS.gene055362 | MS.gene70120 | 0.883101 | 5.97E-71 | -1.69E-46 |
| MS.gene055397 | MS.gene70120 | 0.849083 | 4.06E-60 | -1.69E-46 |
| MS.gene055412 | MS.gene70120 | 0.80055 | 1.37E-48 | -1.69E-46 |
| MS.gene055499 | MS.gene70120 | 0.86494 | 8.47E-65 | -1.69E-46 |
| MS.gene055502 | MS.gene70120 | 0.871669 | 5.73E-67 | -1.69E-46 |
| MS.gene055536 | MS.gene70120 | -0.819072 | 1.41E-52 | -1.69E-46 |
| MS.gene055577 | MS.gene70120 | 0.879587 | 1.10E-69 | -1.69E-46 |
| MS.gene055636 | MS.gene70120 | 0.85612 | 3.99E-62 | -1.69E-46 |
| MS.gene055638 | MS.gene70120 | 0.853632 | 2.10E-61 | -1.69E-46 |
| MS.gene055647 | MS.gene70120 | 0.864231 | 1.41E-64 | -1.69E-46 |
| MS.gene055722 | MS.gene70120 | 0.859351 | 4.39E-63 | -1.69E-46 |
| MS.gene055748 | MS.gene70120 | 0.830419 | 2.97E-55 | -1.69E-46 |
| MS.gene055750 | MS.gene70120 | 0.816319 | 5.88E-52 | -1.69E-46 |
| MS.gene055751 | MS.gene70120 | 0.826377 | 2.81E-54 | -1.69E-46 |
| MS.gene05592 | MS.gene70120 | 0.906975 | 8.35E-81 | -1.69E-46 |
| MS.gene055941 | MS.gene70120 | 0.860149 | 2.53E-63 | -1.69E-46 |
| MS.gene055956 | MS.gene70120 | -0.824858 | 6.43E-54 | -1.69E-46 |
| MS.gene056083 | MS.gene70120 | 0.849951 | 2.32E-60 | -1.69E-46 |
| MS.gene056374 | MS.gene70120 | 0.873168 | 1.81E-67 | -1.69E-46 |
| MS.gene056409 | MS.gene70120 | 0.812874 | 3.40E-51 | -1.69E-46 |
| MS.gene056410 | MS.gene70120 | 0.808197 | 3.49E-50 | -1.69E-46 |
| MS.gene056449 | MS.gene70120 | 0.803998 | 2.67E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene70120 | 0.822392 | 2.43E-53 | -1.69E-46 |
| MS.gene056510 | MS.gene70120 | 0.837207 | 5.96E-57 | -1.69E-46 |
| MS.gene056549 | MS.gene70120 | 0.809682 | 1.68E-50 | -1.69E-46 |
| MS.gene056610 | MS.gene70120 | 0.806304 | 8.78E-50 | -1.69E-46 |
| MS.gene056657 | MS.gene70120 | 0.881835 | 1.73E-70 | -1.69E-46 |
| MS.gene056692 | MS.gene70120 | 0.857658 | 1.41E-62 | -1.69E-46 |
| MS.gene056796 | MS.gene70120 | 0.814559 | 1.45E-51 | -1.69E-46 |
| MS.gene056897 | MS.gene70120 | 0.847513 | 1.10E-59 | -1.69E-46 |
| MS.gene056898 | MS.gene70120 | 0.832009 | 1.21E-55 | -1.69E-46 |
| MS.gene057011 | MS.gene70120 | 0.849161 | 3.86E-60 | -1.69E-46 |
| MS.gene057076 | MS.gene70120 | 0.810412 | 1.17E-50 | -1.69E-46 |
| MS.gene057129 | MS.gene70120 | 0.892183 | 2.01E-74 | -1.69E-46 |
| MS.gene057146 | MS.gene70120 | 0.81858 | 1.82E-52 | -1.69E-46 |
| MS.gene057331 | MS.gene70120 | 0.890714 | 7.67E-74 | -1.69E-46 |
| MS.gene057416 | MS.gene70120 | 0.895724 | 7.30E-76 | -1.69E-46 |
| MS.gene057658 | MS.gene70120 | 0.837565 | 4.82E-57 | -1.69E-46 |
| MS.gene057831 | MS.gene70120 | 0.832151 | 1.11E-55 | -1.69E-46 |
| MS.gene057926 | MS.gene70120 | 0.848506 | 5.87E-60 | -1.69E-46 |
| MS.gene058006 | MS.gene70120 | 0.837075 | 6.44E-57 | -1.69E-46 |
| MS.gene058019 | MS.gene70120 | 0.83662 | 8.41E-57 | -1.69E-46 |
| MS.gene058130 | MS.gene70120 | 0.810152 | 1.33E-50 | -1.69E-46 |
| MS.gene058293 | MS.gene70120 | 0.808755 | 2.65E-50 | -1.69E-46 |
| MS.gene058323 | MS.gene70120 | 0.904523 | 1.12E-79 | -1.69E-46 |
| MS.gene058338 | MS.gene70120 | 0.808635 | 2.81E-50 | -1.69E-46 |
| MS.gene05837 | MS.gene70120 | 0.85001 | 2.24E-60 | -1.69E-46 |
| MS.gene058419 | MS.gene70120 | 0.883044 | 6.26E-71 | -1.69E-46 |
| MS.gene058544 | MS.gene70120 | 0.872925 | 2.19E-67 | -1.69E-46 |
| MS.gene058660 | MS.gene70120 | 0.804266 | 2.35E-49 | -1.69E-46 |
| MS.gene058723 | MS.gene70120 | 0.855455 | 6.24E-62 | -1.69E-46 |
| MS.gene058773 | MS.gene70120 | 0.890472 | 9.55E-74 | -1.69E-46 |
| MS.gene058838 | MS.gene70120 | 0.834401 | 3.06E-56 | -1.69E-46 |
| MS.gene05883 | MS.gene70120 | 0.844951 | 5.50E-59 | -1.69E-46 |
| MS.gene058978 | MS.gene70120 | -0.806614 | 7.55E-50 | -1.69E-46 |
| MS.gene059022 | MS.gene70120 | 0.875337 | 3.34E-68 | -1.69E-46 |
| MS.gene059023 | MS.gene70120 | 0.871569 | 6.19E-67 | -1.69E-46 |
| MS.gene059029 | MS.gene70120 | 0.86294 | 3.55E-64 | -1.69E-46 |
| MS.gene05913 | MS.gene70120 | 0.814361 | 1.60E-51 | -1.69E-46 |
| MS.gene059305 | MS.gene70120 | 0.808605 | 2.85E-50 | -1.69E-46 |
| MS.gene05934 | MS.gene70120 | 0.865185 | 7.09E-65 | -1.69E-46 |
| MS.gene059536 | MS.gene70120 | 0.856728 | 2.64E-62 | -1.69E-46 |
| MS.gene059677 | MS.gene70120 | 0.885271 | 9.39E-72 | -1.69E-46 |
| MS.gene059718 | MS.gene70120 | 0.883491 | 4.29E-71 | -1.69E-46 |
| MS.gene059756 | MS.gene70120 | 0.826812 | 2.21E-54 | -1.69E-46 |
| MS.gene059801 | MS.gene70120 | 0.808365 | 3.21E-50 | -1.69E-46 |
| MS.gene059865 | MS.gene70120 | 0.831138 | 1.98E-55 | -1.69E-46 |
| MS.gene059902 | MS.gene70120 | 0.857584 | 1.48E-62 | -1.69E-46 |
| MS.gene059960 | MS.gene70120 | 0.877234 | 7.40E-69 | -1.69E-46 |
| MS.gene060193 | MS.gene70120 | 0.848662 | 5.31E-60 | -1.69E-46 |
| MS.gene06046 | MS.gene70120 | 0.80429 | 2.32E-49 | -1.69E-46 |
| MS.gene060482 | MS.gene70120 | 0.821937 | 3.10E-53 | -1.69E-46 |
| MS.gene060703 | MS.gene70120 | 0.810689 | 1.02E-50 | -1.69E-46 |
| MS.gene060728 | MS.gene70120 | 0.833853 | 4.20E-56 | -1.69E-46 |
| MS.gene060762 | MS.gene70120 | 0.862588 | 4.56E-64 | -1.69E-46 |
| MS.gene06077 | MS.gene70120 | 0.851556 | 8.22E-61 | -1.69E-46 |
| MS.gene060804 | MS.gene70120 | 0.872218 | 3.76E-67 | -1.69E-46 |
| MS.gene060818 | MS.gene70120 | 0.845808 | 3.22E-59 | -1.69E-46 |
| MS.gene060835 | MS.gene70120 | 0.833972 | 3.92E-56 | -1.69E-46 |
| MS.gene060837 | MS.gene70120 | 0.825823 | 3.80E-54 | -1.69E-46 |
| MS.gene06092 | MS.gene70120 | 0.897939 | 8.64E-77 | -1.69E-46 |
| MS.gene060982 | MS.gene70120 | 0.817607 | 3.02E-52 | -1.69E-46 |
| MS.gene061041 | MS.gene70120 | 0.829886 | 4.01E-55 | -1.69E-46 |
| MS.gene061134 | MS.gene70120 | 0.813721 | 2.22E-51 | -1.69E-46 |
| MS.gene061150 | MS.gene70120 | 0.886284 | 3.91E-72 | -1.69E-46 |
| MS.gene061200 | MS.gene70120 | 0.888688 | 4.73E-73 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70120.t1 | MTR_8g096080 | 91.441 | 222 | 17 | 1 | 1 | 220 | 1 | 222 | 6.97e-152 | 421 |
| MS.gene70120.t1 | MTR_8g096110 | 81.735 | 219 | 40 | 0 | 1 | 219 | 1 | 219 | 3.28e-127 | 358 |
| MS.gene70120.t1 | MTR_8g096100 | 73.113 | 212 | 56 | 1 | 1 | 211 | 1 | 212 | 1.56e-107 | 308 |
| MS.gene70120.t1 | MTR_3g098490 | 66.316 | 190 | 57 | 3 | 1 | 187 | 1 | 186 | 1.90e-89 | 262 |
| MS.gene70120.t1 | MTR_3g098500 | 61.053 | 190 | 71 | 2 | 1 | 187 | 7 | 196 | 5.31e-82 | 244 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70120.t1 | AT3G23760 | 61.622 | 185 | 68 | 2 | 9 | 191 | 11 | 194 | 7.69e-83 | 245 |
| MS.gene70120.t1 | AT4G14100 | 55.051 | 198 | 88 | 1 | 9 | 206 | 8 | 204 | 6.57e-80 | 238 |
Find 58 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTCCTTCTCTTGTGTATT+TGG | 0.089272 | 4.1:+7225153 | None:intergenic |
| GCTTGATGGTGCTAATTATT+TGG | 0.169784 | 4.1:-7225494 | MS.gene70120:CDS |
| GCTCCTTCTCTTGTGTATTT+GGG | 0.191163 | 4.1:+7225154 | None:intergenic |
| TCCGGCCACGGCGCCGGAAT+TGG | 0.215529 | 4.1:+7225745 | None:intergenic |
| GGGAGAAAGTCGAATTTATT+TGG | 0.267906 | 4.1:-7225435 | MS.gene70120:CDS |
| CCACGACCCAATTGGCTTGA+TGG | 0.304709 | 4.1:-7225508 | MS.gene70120:CDS |
| CTTGATGGTGCTAATTATTT+GGG | 0.312059 | 4.1:-7225493 | MS.gene70120:CDS |
| CCGGCCACGGCGCCGGAATT+GGG | 0.367218 | 4.1:+7225746 | None:intergenic |
| ATTTGAGGTTGGAGCAGTTC+TGG | 0.367268 | 4.1:-7225231 | MS.gene70120:CDS |
| TGAGAACATCAAGCTGGTAT+TGG | 0.386461 | 4.1:+7225626 | None:intergenic |
| AGGACTGAGTGGAACTGTTC+CGG | 0.404511 | 4.1:+7225727 | None:intergenic |
| ATTAGCACCATCAAGCCAAT+TGG | 0.405118 | 4.1:+7225501 | None:intergenic |
| GACCACAGAGGACCCAATTC+CGG | 0.405830 | 4.1:-7225758 | MS.gene70120:CDS |
| AATGTTGAAATTTCGTCCAT+TGG | 0.430457 | 4.1:+7225651 | None:intergenic |
| ATGTTGAAATTTCGTCCATT+GGG | 0.444878 | 4.1:+7225652 | None:intergenic |
| CTCTTCGAAAACAAGACTTA+TGG | 0.444999 | 4.1:-7225684 | MS.gene70120:CDS |
| AACTGTTCCGGCCACGGCGC+CGG | 0.448851 | 4.1:+7225739 | None:intergenic |
| CCCAATTCCGGCGCCGTGGC+CGG | 0.459468 | 4.1:-7225746 | MS.gene70120:CDS |
| TTGGTATTCCACGACCCAAT+TGG | 0.459870 | 4.1:-7225516 | MS.gene70120:CDS |
| CTCCTTCTCTTGTGTATTTG+GGG | 0.462628 | 4.1:+7225155 | None:intergenic |
| AACATTACACAGAAAATTGT+CGG | 0.472163 | 4.1:+7225459 | None:intergenic |
| TCTGGATGATGCCCATTGGC+AGG | 0.480962 | 4.1:-7225213 | MS.gene70120:CDS |
| ACTAATGAGTGAGATGAGAT+AGG | 0.485469 | 4.1:-7225081 | None:intergenic |
| AGCCGCTTCTGTTGAGGAGT+AGG | 0.512649 | 4.1:+7225707 | None:intergenic |
| TTCTCAAATATGACCTTGCA+TGG | 0.513063 | 4.1:-7225609 | MS.gene70120:CDS |
| ACTTTGCAAGTGTGCTCGAA+AGG | 0.514114 | 4.1:+7225553 | None:intergenic |
| TTAGCACCATCAAGCCAATT+GGG | 0.517591 | 4.1:+7225502 | None:intergenic |
| AACAAGACTTATGGTACGAT+TGG | 0.522229 | 4.1:-7225675 | MS.gene70120:CDS |
| GAGTGGAACTGTTCCGGCCA+CGG | 0.525676 | 4.1:+7225733 | None:intergenic |
| TGGTCGAAGTTGAGAGATGA+AGG | 0.525737 | 4.1:+7225776 | None:intergenic |
| GAAGAGAGCCGCTTCTGTTG+AGG | 0.544198 | 4.1:+7225701 | None:intergenic |
| TACTGATGTGATGACATTTG+AGG | 0.549841 | 4.1:-7225246 | MS.gene70120:CDS |
| AATGCATTCTTCTGTGGCAG+AGG | 0.552048 | 4.1:+7225825 | None:intergenic |
| AATAGACAGGAGCCTGCCAA+TGG | 0.552630 | 4.1:+7225201 | None:intergenic |
| TTATGGTACGATTGGCCCAA+TGG | 0.556501 | 4.1:-7225667 | MS.gene70120:CDS |
| CTTTGCAAGTGTGCTCGAAA+GGG | 0.564893 | 4.1:+7225554 | None:intergenic |
| AAGTTGAGAGATGAAGGAAG+AGG | 0.569210 | 4.1:+7225782 | None:intergenic |
| TATGACCTTGCATGGAACAA+TGG | 0.569774 | 4.1:-7225601 | MS.gene70120:CDS |
| CCATCAAGCCAATTGGGTCG+TGG | 0.576651 | 4.1:+7225508 | None:intergenic |
| ATAGACAGGAGCCTGCCAAT+GGG | 0.577912 | 4.1:+7225202 | None:intergenic |
| GTCCTACTCCTCAACAGAAG+CGG | 0.579821 | 4.1:-7225709 | MS.gene70120:CDS |
| AACCCCAAATACACAAGAGA+AGG | 0.582352 | 4.1:-7225157 | MS.gene70120:CDS |
| TGGTGCCATTGTTCCATGCA+AGG | 0.590215 | 4.1:+7225596 | None:intergenic |
| TCGTAGAAGATCCACTTGAC+AGG | 0.590706 | 4.1:+7225385 | None:intergenic |
| TCACTAAAAGACCTGTCAAG+TGG | 0.590795 | 4.1:-7225396 | MS.gene70120:CDS |
| CAGTTCTGGATGATGCCCAT+TGG | 0.593169 | 4.1:-7225217 | MS.gene70120:CDS |
| GCCTTACTAAAACAATAGAC+AGG | 0.594404 | 4.1:+7225188 | None:intergenic |
| CATATTTGAGAACATCAAGC+TGG | 0.600302 | 4.1:+7225620 | None:intergenic |
| AGGGTCGAGAGTGTAGAGAA+AGG | 0.604953 | 4.1:+7225573 | None:intergenic |
| CAAACAAATGCATTCTTCTG+TGG | 0.604964 | 4.1:+7225819 | None:intergenic |
| CGCCGGAATTGGGTCCTCTG+TGG | 0.626171 | 4.1:+7225756 | None:intergenic |
| GATGTGATGACATTTGAGGT+TGG | 0.629445 | 4.1:-7225242 | MS.gene70120:CDS |
| GTCAAGTGGATCTTCTACGA+TGG | 0.629602 | 4.1:-7225382 | MS.gene70120:intron |
| AGGACCCAATTCCGGCGCCG+TGG | 0.635409 | 4.1:-7225750 | MS.gene70120:CDS |
| AAAGTGACTCAATATGATGT+TGG | 0.647948 | 4.1:-7225535 | MS.gene70120:CDS |
| TGTTGAGGAGTAGGACTGAG+TGG | 0.648618 | 4.1:+7225716 | None:intergenic |
| GTCGAGAGTGTAGAGAAAGG+TGG | 0.677328 | 4.1:+7225576 | None:intergenic |
| TCTCTCAACTTCGACCACAG+AGG | 0.720290 | 4.1:-7225770 | MS.gene70120:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATTCATTATATGATGAT+TGG | - | chr4.1:7225626-7225645 | MS.gene70120:CDS | 15.0% |
| ! | AACATTACACAGAAAATTGT+CGG | + | chr4.1:7225455-7225474 | None:intergenic | 25.0% |
| ! | CTACATATAGCTAAAATTAC+TGG | - | chr4.1:7225580-7225599 | MS.gene70120:CDS | 25.0% |
| !! | ACAATTTTCTGTGTAATGTT+TGG | - | chr4.1:7225455-7225474 | MS.gene70120:CDS | 25.0% |
| !! | CAATTTTCTGTGTAATGTTT+GGG | - | chr4.1:7225456-7225475 | MS.gene70120:CDS | 25.0% |
| AAAGTGACTCAATATGATGT+TGG | - | chr4.1:7225376-7225395 | MS.gene70120:intron | 30.0% | |
| AATGTTGAAATTTCGTCCAT+TGG | + | chr4.1:7225263-7225282 | None:intergenic | 30.0% | |
| ATGTTGAAATTTCGTCCATT+GGG | + | chr4.1:7225262-7225281 | None:intergenic | 30.0% | |
| !! | CTTGATGGTGCTAATTATTT+GGG | - | chr4.1:7225418-7225437 | MS.gene70120:CDS | 30.0% |
| !!! | CTATTGTTGATGATTTTCGT+TGG | - | chr4.1:7225805-7225824 | MS.gene70120:CDS | 30.0% |
| !!! | TCCTGTCTATTGTTTTAGTA+AGG | - | chr4.1:7225722-7225741 | MS.gene70120:CDS | 30.0% |
| AACAAGACTTATGGTACGAT+TGG | - | chr4.1:7225236-7225255 | MS.gene70120:CDS | 35.0% | |
| CAAACAAATGCATTCTTCTG+TGG | + | chr4.1:7225095-7225114 | None:intergenic | 35.0% | |
| CATATTTGAGAACATCAAGC+TGG | + | chr4.1:7225294-7225313 | None:intergenic | 35.0% | |
| CTCTTCGAAAACAAGACTTA+TGG | - | chr4.1:7225227-7225246 | MS.gene70120:CDS | 35.0% | |
| GCCTTACTAAAACAATAGAC+AGG | + | chr4.1:7225726-7225745 | None:intergenic | 35.0% | |
| GGGAGAAAGTCGAATTTATT+TGG | - | chr4.1:7225476-7225495 | MS.gene70120:CDS | 35.0% | |
| TACTGATGTGATGACATTTG+AGG | - | chr4.1:7225665-7225684 | MS.gene70120:CDS | 35.0% | |
| TTCTCAAATATGACCTTGCA+TGG | - | chr4.1:7225302-7225321 | MS.gene70120:intron | 35.0% | |
| !! | GCTTGATGGTGCTAATTATT+TGG | - | chr4.1:7225417-7225436 | MS.gene70120:CDS | 35.0% |
| !!! | CTATTGTTTTAGTAAGGCTG+AGG | - | chr4.1:7225728-7225747 | MS.gene70120:CDS | 35.0% |
| AACCCCAAATACACAAGAGA+AGG | - | chr4.1:7225754-7225773 | MS.gene70120:CDS | 40.0% | |
| AAGTTGAGAGATGAAGGAAG+AGG | + | chr4.1:7225132-7225151 | None:intergenic | 40.0% | |
| ATATGATGATTGGTGAGTGC+AGG | - | chr4.1:7225636-7225655 | MS.gene70120:CDS | 40.0% | |
| ATTAGCACCATCAAGCCAAT+TGG | + | chr4.1:7225413-7225432 | None:intergenic | 40.0% | |
| CTCCTTCTCTTGTGTATTTG+GGG | + | chr4.1:7225759-7225778 | None:intergenic | 40.0% | |
| GCTCCTTCTCTTGTGTATTT+GGG | + | chr4.1:7225760-7225779 | None:intergenic | 40.0% | |
| TATGACCTTGCATGGAACAA+TGG | - | chr4.1:7225310-7225329 | MS.gene70120:intron | 40.0% | |
| TCACTAAAAGACCTGTCAAG+TGG | - | chr4.1:7225515-7225534 | MS.gene70120:CDS | 40.0% | |
| TGAGAACATCAAGCTGGTAT+TGG | + | chr4.1:7225288-7225307 | None:intergenic | 40.0% | |
| TGCTCCTTCTCTTGTGTATT+TGG | + | chr4.1:7225761-7225780 | None:intergenic | 40.0% | |
| TTAGCACCATCAAGCCAATT+GGG | + | chr4.1:7225412-7225431 | None:intergenic | 40.0% | |
| ! | GATGTGATGACATTTGAGGT+TGG | - | chr4.1:7225669-7225688 | MS.gene70120:CDS | 40.0% |
| AATGCATTCTTCTGTGGCAG+AGG | + | chr4.1:7225089-7225108 | None:intergenic | 45.0% | |
| ACTTTGCAAGTGTGCTCGAA+AGG | + | chr4.1:7225361-7225380 | None:intergenic | 45.0% | |
| CTTTGCAAGTGTGCTCGAAA+GGG | + | chr4.1:7225360-7225379 | None:intergenic | 45.0% | |
| GTCAAGTGGATCTTCTACGA+TGG | - | chr4.1:7225529-7225548 | MS.gene70120:CDS | 45.0% | |
| TCGTAGAAGATCCACTTGAC+AGG | + | chr4.1:7225529-7225548 | None:intergenic | 45.0% | |
| TGGTCGAAGTTGAGAGATGA+AGG | + | chr4.1:7225138-7225157 | None:intergenic | 45.0% | |
| TTGGTATTCCACGACCCAAT+TGG | - | chr4.1:7225395-7225414 | MS.gene70120:CDS | 45.0% | |
| ! | ATTTGAGGTTGGAGCAGTTC+TGG | - | chr4.1:7225680-7225699 | MS.gene70120:CDS | 45.0% |
| ! | TTATGGTACGATTGGCCCAA+TGG | - | chr4.1:7225244-7225263 | MS.gene70120:CDS | 45.0% |
| AATAGACAGGAGCCTGCCAA+TGG | + | chr4.1:7225713-7225732 | None:intergenic | 50.0% | |
| AGGACTGAGTGGAACTGTTC+CGG | + | chr4.1:7225187-7225206 | None:intergenic | 50.0% | |
| ATAGACAGGAGCCTGCCAAT+GGG | + | chr4.1:7225712-7225731 | None:intergenic | 50.0% | |
| GTCCTACTCCTCAACAGAAG+CGG | - | chr4.1:7225202-7225221 | MS.gene70120:CDS | 50.0% | |
| TCTCTCAACTTCGACCACAG+AGG | - | chr4.1:7225141-7225160 | MS.gene70120:CDS | 50.0% | |
| TGTTGAGGAGTAGGACTGAG+TGG | + | chr4.1:7225198-7225217 | None:intergenic | 50.0% | |
| ! | AGGGTCGAGAGTGTAGAGAA+AGG | + | chr4.1:7225341-7225360 | None:intergenic | 50.0% |
| ! | GTCGAGAGTGTAGAGAAAGG+TGG | + | chr4.1:7225338-7225357 | None:intergenic | 50.0% |
| !! | CAGTTCTGGATGATGCCCAT+TGG | - | chr4.1:7225694-7225713 | MS.gene70120:CDS | 50.0% |
| !! | TGGTGCCATTGTTCCATGCA+AGG | + | chr4.1:7225318-7225337 | None:intergenic | 50.0% |
| AGCCGCTTCTGTTGAGGAGT+AGG | + | chr4.1:7225207-7225226 | None:intergenic | 55.0% | |
| CCATCAAGCCAATTGGGTCG+TGG | + | chr4.1:7225406-7225425 | None:intergenic | 55.0% | |
| GAAGAGAGCCGCTTCTGTTG+AGG | + | chr4.1:7225213-7225232 | None:intergenic | 55.0% | |
| GACCACAGAGGACCCAATTC+CGG | - | chr4.1:7225153-7225172 | MS.gene70120:CDS | 55.0% | |
| TCTGGATGATGCCCATTGGC+AGG | - | chr4.1:7225698-7225717 | MS.gene70120:CDS | 55.0% | |
| !! | CCACGACCCAATTGGCTTGA+TGG | - | chr4.1:7225403-7225422 | MS.gene70120:CDS | 55.0% |
| GAGTGGAACTGTTCCGGCCA+CGG | + | chr4.1:7225181-7225200 | None:intergenic | 60.0% | |
| CGCCGGAATTGGGTCCTCTG+TGG | + | chr4.1:7225158-7225177 | None:intergenic | 65.0% | |
| AACTGTTCCGGCCACGGCGC+CGG | + | chr4.1:7225175-7225194 | None:intergenic | 70.0% | |
| AGGACCCAATTCCGGCGCCG+TGG | - | chr4.1:7225161-7225180 | MS.gene70120:CDS | 70.0% | |
| CCCAATTCCGGCGCCGTGGC+CGG | - | chr4.1:7225165-7225184 | MS.gene70120:CDS | 75.0% | |
| ! | CCGGCCACGGCGCCGGAATT+GGG | + | chr4.1:7225168-7225187 | None:intergenic | 75.0% |
| ! | TCCGGCCACGGCGCCGGAAT+TGG | + | chr4.1:7225169-7225188 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 7225082 | 7225851 | 7225082 | ID=MS.gene70120 |
| chr4.1 | mRNA | 7225082 | 7225851 | 7225082 | ID=MS.gene70120.t1;Parent=MS.gene70120 |
| chr4.1 | exon | 7225383 | 7225851 | 7225383 | ID=MS.gene70120.t1.exon1;Parent=MS.gene70120.t1 |
| chr4.1 | CDS | 7225383 | 7225851 | 7225383 | ID=cds.MS.gene70120.t1;Parent=MS.gene70120.t1 |
| chr4.1 | exon | 7225082 | 7225275 | 7225082 | ID=MS.gene70120.t1.exon2;Parent=MS.gene70120.t1 |
| chr4.1 | CDS | 7225082 | 7225275 | 7225082 | ID=cds.MS.gene70120.t1;Parent=MS.gene70120.t1 |
| Gene Sequence |
| Protein sequence |
