Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76453.t1 | XP_013446375.1 | 97.5 | 320 | 8 | 0 | 1 | 320 | 1 | 320 | 4.80E-186 | 660.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76453.t1 | I6YGS0 | 37.1 | 329 | 187 | 7 | 3 | 319 | 1 | 321 | 3.7e-54 | 213.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76453.t1 | A0A072TSN0 | 97.5 | 320 | 8 | 0 | 1 | 320 | 1 | 320 | 3.5e-186 | 660.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene76453 | -0.800292 | 1.54E-48 | -1.69E-46 |
| MS.gene049232 | MS.gene76453 | -0.815616 | 8.44E-52 | -1.69E-46 |
| MS.gene049259 | MS.gene76453 | 0.824936 | 6.16E-54 | -1.69E-46 |
| MS.gene049281 | MS.gene76453 | 0.801469 | 8.87E-49 | -1.69E-46 |
| MS.gene049285 | MS.gene76453 | -0.800291 | 1.54E-48 | -1.69E-46 |
| MS.gene049320 | MS.gene76453 | -0.816333 | 5.84E-52 | -1.69E-46 |
| MS.gene049611 | MS.gene76453 | 0.828256 | 9.95E-55 | -1.69E-46 |
| MS.gene049634 | MS.gene76453 | 0.820522 | 6.56E-53 | -1.69E-46 |
| MS.gene049710 | MS.gene76453 | 0.807081 | 6.02E-50 | -1.69E-46 |
| MS.gene04986 | MS.gene76453 | 0.840516 | 8.30E-58 | -1.69E-46 |
| MS.gene050082 | MS.gene76453 | 0.808036 | 3.77E-50 | -1.69E-46 |
| MS.gene05010 | MS.gene76453 | -0.803912 | 2.78E-49 | -1.69E-46 |
| MS.gene05015 | MS.gene76453 | 0.841769 | 3.89E-58 | -1.69E-46 |
| MS.gene050173 | MS.gene76453 | 0.828342 | 9.48E-55 | -1.69E-46 |
| MS.gene050216 | MS.gene76453 | -0.844572 | 6.96E-59 | -1.69E-46 |
| MS.gene050323 | MS.gene76453 | -0.801234 | 9.91E-49 | -1.69E-46 |
| MS.gene050333 | MS.gene76453 | -0.818054 | 2.39E-52 | -1.69E-46 |
| MS.gene050381 | MS.gene76453 | 0.859249 | 4.71E-63 | -1.69E-46 |
| MS.gene050496 | MS.gene76453 | -0.814891 | 1.22E-51 | -1.69E-46 |
| MS.gene050504 | MS.gene76453 | -0.824296 | 8.72E-54 | -1.69E-46 |
| MS.gene050612 | MS.gene76453 | -0.831675 | 1.46E-55 | -1.69E-46 |
| MS.gene050868 | MS.gene76453 | -0.825815 | 3.81E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene76453 | -0.823042 | 1.71E-53 | -1.69E-46 |
| MS.gene050883 | MS.gene76453 | 0.840501 | 8.37E-58 | -1.69E-46 |
| MS.gene051044 | MS.gene76453 | 0.824341 | 8.51E-54 | -1.69E-46 |
| MS.gene05104 | MS.gene76453 | 0.828666 | 7.92E-55 | -1.69E-46 |
| MS.gene051127 | MS.gene76453 | 0.847068 | 1.46E-59 | -1.69E-46 |
| MS.gene051138 | MS.gene76453 | 0.852357 | 4.87E-61 | -1.69E-46 |
| MS.gene051364 | MS.gene76453 | 0.853574 | 2.18E-61 | -1.69E-46 |
| MS.gene051366 | MS.gene76453 | 0.868652 | 5.57E-66 | -1.69E-46 |
| MS.gene051367 | MS.gene76453 | 0.851552 | 8.24E-61 | -1.69E-46 |
| MS.gene051946 | MS.gene76453 | 0.816912 | 4.33E-52 | -1.69E-46 |
| MS.gene052916 | MS.gene76453 | -0.80766 | 4.53E-50 | -1.69E-46 |
| MS.gene053420 | MS.gene76453 | 0.864215 | 1.43E-64 | -1.69E-46 |
| MS.gene053488 | MS.gene76453 | 0.818013 | 2.45E-52 | -1.69E-46 |
| MS.gene053561 | MS.gene76453 | 0.870837 | 1.08E-66 | -1.69E-46 |
| MS.gene054054 | MS.gene76453 | 0.812085 | 5.06E-51 | -1.69E-46 |
| MS.gene054056 | MS.gene76453 | 0.83505 | 2.10E-56 | -1.69E-46 |
| MS.gene054616 | MS.gene76453 | 0.812334 | 4.47E-51 | -1.69E-46 |
| MS.gene054707 | MS.gene76453 | 0.806668 | 7.36E-50 | -1.69E-46 |
| MS.gene054708 | MS.gene76453 | 0.843414 | 1.42E-58 | -1.69E-46 |
| MS.gene054954 | MS.gene76453 | -0.815659 | 8.26E-52 | -1.69E-46 |
| MS.gene055111 | MS.gene76453 | 0.809462 | 1.87E-50 | -1.69E-46 |
| MS.gene055124 | MS.gene76453 | 0.856448 | 3.20E-62 | -1.69E-46 |
| MS.gene055139 | MS.gene76453 | 0.815363 | 9.61E-52 | -1.69E-46 |
| MS.gene055154 | MS.gene76453 | 0.880726 | 4.33E-70 | -1.69E-46 |
| MS.gene055202 | MS.gene76453 | 0.839657 | 1.39E-57 | -1.69E-46 |
| MS.gene055362 | MS.gene76453 | -0.816714 | 4.80E-52 | -1.69E-46 |
| MS.gene055502 | MS.gene76453 | -0.809464 | 1.87E-50 | -1.69E-46 |
| MS.gene055776 | MS.gene76453 | -0.823016 | 1.74E-53 | -1.69E-46 |
| MS.gene05589 | MS.gene76453 | -0.807481 | 4.95E-50 | -1.69E-46 |
| MS.gene055942 | MS.gene76453 | 0.854824 | 9.52E-62 | -1.69E-46 |
| MS.gene056185 | MS.gene76453 | 0.804255 | 2.36E-49 | -1.69E-46 |
| MS.gene056224 | MS.gene76453 | 0.806136 | 9.52E-50 | -1.69E-46 |
| MS.gene056311 | MS.gene76453 | 0.827786 | 1.29E-54 | -1.69E-46 |
| MS.gene056313 | MS.gene76453 | 0.800876 | 1.17E-48 | -1.69E-46 |
| MS.gene056504 | MS.gene76453 | -0.839629 | 1.41E-57 | -1.69E-46 |
| MS.gene056510 | MS.gene76453 | -0.800817 | 1.21E-48 | -1.69E-46 |
| MS.gene056657 | MS.gene76453 | -0.805856 | 1.09E-49 | -1.69E-46 |
| MS.gene056761 | MS.gene76453 | 0.814403 | 1.57E-51 | -1.69E-46 |
| MS.gene056856 | MS.gene76453 | 0.8081 | 3.66E-50 | -1.69E-46 |
| MS.gene057014 | MS.gene76453 | 0.840634 | 7.73E-58 | -1.69E-46 |
| MS.gene057473 | MS.gene76453 | 0.804731 | 1.88E-49 | -1.69E-46 |
| MS.gene057549 | MS.gene76453 | 0.826211 | 3.07E-54 | -1.69E-46 |
| MS.gene058068 | MS.gene76453 | 0.819463 | 1.15E-52 | -1.69E-46 |
| MS.gene058093 | MS.gene76453 | 0.822316 | 2.53E-53 | -1.69E-46 |
| MS.gene058099 | MS.gene76453 | 0.855547 | 5.86E-62 | -1.69E-46 |
| MS.gene058108 | MS.gene76453 | 0.823423 | 1.40E-53 | -1.69E-46 |
| MS.gene058109 | MS.gene76453 | 0.855828 | 4.86E-62 | -1.69E-46 |
| MS.gene058111 | MS.gene76453 | 0.834824 | 2.40E-56 | -1.69E-46 |
| MS.gene058117 | MS.gene76453 | 0.815578 | 8.60E-52 | -1.69E-46 |
| MS.gene058171 | MS.gene76453 | 0.861576 | 9.31E-64 | -1.69E-46 |
| MS.gene058173 | MS.gene76453 | 0.847199 | 1.34E-59 | -1.69E-46 |
| MS.gene058175 | MS.gene76453 | 0.863086 | 3.20E-64 | -1.69E-46 |
| MS.gene058268 | MS.gene76453 | 0.831365 | 1.74E-55 | -1.69E-46 |
| MS.gene058269 | MS.gene76453 | 0.830485 | 2.86E-55 | -1.69E-46 |
| MS.gene058347 | MS.gene76453 | 0.821397 | 4.13E-53 | -1.69E-46 |
| MS.gene058349 | MS.gene76453 | 0.800192 | 1.62E-48 | -1.69E-46 |
| MS.gene058493 | MS.gene76453 | 0.846744 | 1.79E-59 | -1.69E-46 |
| MS.gene058494 | MS.gene76453 | 0.825548 | 4.42E-54 | -1.69E-46 |
| MS.gene058628 | MS.gene76453 | 0.816622 | 5.03E-52 | -1.69E-46 |
| MS.gene058629 | MS.gene76453 | 0.80138 | 9.25E-49 | -1.69E-46 |
| MS.gene058630 | MS.gene76453 | 0.803733 | 3.03E-49 | -1.69E-46 |
| MS.gene058773 | MS.gene76453 | -0.823661 | 1.23E-53 | -1.69E-46 |
| MS.gene059022 | MS.gene76453 | -0.846246 | 2.45E-59 | -1.69E-46 |
| MS.gene059045 | MS.gene76453 | 0.823869 | 1.10E-53 | -1.69E-46 |
| MS.gene059677 | MS.gene76453 | -0.807989 | 3.86E-50 | -1.69E-46 |
| MS.gene059690 | MS.gene76453 | 0.829262 | 5.68E-55 | -1.69E-46 |
| MS.gene059691 | MS.gene76453 | 0.807202 | 5.67E-50 | -1.69E-46 |
| MS.gene059694 | MS.gene76453 | 0.827737 | 1.33E-54 | -1.69E-46 |
| MS.gene059697 | MS.gene76453 | 0.82554 | 4.43E-54 | -1.69E-46 |
| MS.gene059865 | MS.gene76453 | -0.845483 | 3.95E-59 | -1.69E-46 |
| MS.gene059902 | MS.gene76453 | -0.801239 | 9.89E-49 | -1.69E-46 |
| MS.gene060482 | MS.gene76453 | -0.801745 | 7.79E-49 | -1.69E-46 |
| MS.gene060595 | MS.gene76453 | 0.830223 | 3.31E-55 | -1.69E-46 |
| MS.gene060762 | MS.gene76453 | -0.822624 | 2.14E-53 | -1.69E-46 |
| MS.gene060778 | MS.gene76453 | -0.800112 | 1.68E-48 | -1.69E-46 |
| MS.gene06077 | MS.gene76453 | -0.81855 | 1.85E-52 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76453.t1 | MTR_8g076680 | 97.500 | 320 | 8 | 0 | 1 | 320 | 1 | 320 | 0.0 | 647 |
| MS.gene76453.t1 | MTR_8g076680 | 97.899 | 238 | 5 | 0 | 1 | 238 | 1 | 238 | 8.47e-177 | 488 |
| MS.gene76453.t1 | MTR_7g112963 | 47.368 | 323 | 158 | 4 | 3 | 317 | 1 | 319 | 7.54e-109 | 320 |
| MS.gene76453.t1 | MTR_7g113030 | 47.988 | 323 | 156 | 4 | 3 | 317 | 1 | 319 | 1.43e-108 | 319 |
| MS.gene76453.t1 | MTR_4g120240 | 47.040 | 321 | 166 | 3 | 1 | 317 | 35 | 355 | 5.01e-108 | 319 |
| MS.gene76453.t1 | MTR_7g113050 | 48.276 | 319 | 161 | 3 | 3 | 317 | 1 | 319 | 5.92e-108 | 317 |
| MS.gene76453.t1 | MTR_7g112960 | 46.440 | 323 | 161 | 5 | 3 | 317 | 1 | 319 | 8.12e-108 | 317 |
| MS.gene76453.t1 | MTR_7g112615 | 47.649 | 319 | 163 | 3 | 3 | 317 | 1 | 319 | 7.18e-107 | 315 |
| MS.gene76453.t1 | MTR_7g112970 | 47.188 | 320 | 160 | 5 | 3 | 317 | 1 | 316 | 1.17e-106 | 314 |
| MS.gene76453.t1 | MTR_7g108825 | 47.003 | 317 | 166 | 2 | 3 | 317 | 1 | 317 | 1.26e-106 | 314 |
| MS.gene76453.t1 | MTR_7g113020 | 46.708 | 319 | 166 | 3 | 3 | 317 | 1 | 319 | 1.98e-106 | 313 |
| MS.gene76453.t1 | MTR_7g112620 | 47.335 | 319 | 164 | 3 | 3 | 317 | 1 | 319 | 2.02e-105 | 311 |
| MS.gene76453.t1 | MTR_4g005160 | 44.410 | 322 | 172 | 5 | 3 | 317 | 1 | 322 | 1.28e-95 | 286 |
| MS.gene76453.t1 | MTR_7g113010 | 45.614 | 285 | 145 | 3 | 3 | 281 | 1 | 281 | 3.35e-89 | 278 |
| MS.gene76453.t1 | MTR_7g113050 | 49.580 | 238 | 118 | 2 | 3 | 238 | 1 | 238 | 1.97e-81 | 247 |
| MS.gene76453.t1 | MTR_7g108825 | 48.729 | 236 | 121 | 0 | 3 | 238 | 1 | 236 | 2.06e-80 | 244 |
| MS.gene76453.t1 | MTR_7g112615 | 48.739 | 238 | 120 | 2 | 3 | 238 | 1 | 238 | 3.25e-80 | 244 |
| MS.gene76453.t1 | MTR_4g089060 | 38.050 | 318 | 184 | 6 | 3 | 316 | 1 | 309 | 2.17e-73 | 229 |
| MS.gene76453.t1 | MTR_4g089040 | 37.736 | 318 | 185 | 6 | 3 | 316 | 1 | 309 | 4.63e-71 | 223 |
| MS.gene76453.t1 | MTR_4g089060 | 35.072 | 345 | 184 | 7 | 3 | 316 | 1 | 336 | 3.02e-68 | 216 |
| MS.gene76453.t1 | MTR_2g090240 | 35.202 | 321 | 196 | 6 | 3 | 319 | 1 | 313 | 1.55e-66 | 211 |
| MS.gene76453.t1 | MTR_2g090245 | 34.268 | 321 | 199 | 6 | 3 | 319 | 1 | 313 | 5.79e-65 | 207 |
| MS.gene76453.t1 | MTR_1g083480 | 53.797 | 158 | 70 | 1 | 3 | 157 | 1 | 158 | 2.21e-59 | 188 |
| MS.gene76453.t1 | MTR_7g112615 | 52.201 | 159 | 75 | 1 | 3 | 160 | 1 | 159 | 2.59e-59 | 188 |
| MS.gene76453.t1 | MTR_4g089060 | 39.827 | 231 | 132 | 3 | 3 | 231 | 1 | 226 | 2.03e-58 | 188 |
| MS.gene76453.t1 | MTR_3g024250 | 51.429 | 105 | 51 | 0 | 49 | 153 | 3 | 107 | 6.69e-32 | 116 |
| MS.gene76453.t1 | MTR_7g112910 | 30.769 | 247 | 111 | 8 | 76 | 317 | 4 | 195 | 3.76e-25 | 100 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76453.t1 | AT3G51000 | 63.323 | 319 | 112 | 4 | 4 | 318 | 3 | 320 | 2.87e-152 | 430 |
| MS.gene76453.t1 | AT4G02340 | 47.812 | 320 | 158 | 5 | 3 | 317 | 1 | 316 | 5.20e-107 | 315 |
| MS.gene76453.t1 | AT3G05600 | 46.341 | 328 | 159 | 6 | 3 | 317 | 1 | 324 | 1.46e-100 | 299 |
| MS.gene76453.t1 | AT2G26740 | 47.664 | 321 | 159 | 5 | 6 | 317 | 1 | 321 | 1.39e-91 | 276 |
| MS.gene76453.t1 | AT4G15960 | 41.358 | 324 | 181 | 4 | 3 | 317 | 51 | 374 | 5.01e-88 | 268 |
| MS.gene76453.t1 | AT2G26750 | 46.372 | 317 | 162 | 5 | 6 | 314 | 1 | 317 | 7.92e-88 | 266 |
| MS.gene76453.t1 | AT4G15955 | 41.325 | 317 | 148 | 8 | 7 | 310 | 6 | 297 | 1.33e-80 | 247 |
| MS.gene76453.t1 | AT3G05600 | 46.559 | 247 | 117 | 5 | 3 | 238 | 1 | 243 | 3.21e-74 | 229 |
| MS.gene76453.t1 | AT4G15955 | 50.289 | 173 | 75 | 3 | 7 | 168 | 6 | 178 | 2.49e-56 | 181 |
| MS.gene76453.t1 | AT4G15955 | 50.303 | 165 | 71 | 3 | 7 | 160 | 6 | 170 | 3.52e-53 | 172 |
Find 84 sgRNAs with CRISPR-Local
Find 128 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACAGCATGATAACCATTT+TGG | 0.142448 | 8.4:+29017799 | None:intergenic |
| ATTGGCCTTCTTGATCATTT+TGG | 0.170509 | 8.4:-29017693 | MS.gene76453:CDS |
| CGCCAAGAATACCATATTTC+TGG | 0.182663 | 8.4:+29017843 | None:intergenic |
| AGCTCATGTTTGCCAATTTC+AGG | 0.222501 | 8.4:-29017171 | MS.gene76453:intron |
| TTCATGGTTTCCCAGAAATA+TGG | 0.287073 | 8.4:-29017854 | MS.gene76453:CDS |
| CTCAACTACTTGGCCCAAAA+TGG | 0.297865 | 8.4:-29017813 | MS.gene76453:CDS |
| CCTAATCTAGAGATAGTTTA+CGG | 0.303872 | 8.4:+29016516 | None:intergenic |
| AAGGGTAATCTACCTGAAAT+TGG | 0.307281 | 8.4:+29017159 | None:intergenic |
| TCCCAGAAATATGGTATTCT+TGG | 0.309032 | 8.4:-29017845 | MS.gene76453:CDS |
| GTGTAGGAATTGGGGTTGAT+AGG | 0.327908 | 8.4:+29017741 | None:intergenic |
| CCACTAGTTCTACTCCTTCA+TGG | 0.349801 | 8.4:-29017870 | MS.gene76453:CDS |
| CACATCGTAGGTGACCTTAT+TGG | 0.355687 | 8.4:-29017711 | MS.gene76453:CDS |
| GATGGCACCTAAGTCTATTT+AGG | 0.355832 | 8.4:-29017295 | MS.gene76453:CDS |
| CCGTAAACTATCTCTAGATT+AGG | 0.356075 | 8.4:-29016516 | None:intergenic |
| TGATGAAGAGTGTAGGAATT+GGG | 0.368332 | 8.4:+29017732 | None:intergenic |
| ACAAACCCCTTCACTCTATC+AGG | 0.370013 | 8.4:+29017272 | None:intergenic |
| CGTAAACTATCTCTAGATTA+GGG | 0.379541 | 8.4:-29016515 | None:intergenic |
| TCTCCGGCAATAAACTTAGT+TGG | 0.388206 | 8.4:+29016698 | None:intergenic |
| TGATTTCTTGCCAACTCCTT+CGG | 0.394280 | 8.4:-29016946 | MS.gene76453:CDS |
| GTTCCAACTAAGTTTATTGC+CGG | 0.395155 | 8.4:-29016701 | MS.gene76453:CDS |
| GCAGACAAATTCCAAGAGTC+TGG | 0.396715 | 8.4:-29016882 | MS.gene76453:CDS |
| AATGATCAAGAAGGCCAATA+AGG | 0.412797 | 8.4:+29017697 | None:intergenic |
| CACATGTATATTTATTCCGT+TGG | 0.415186 | 8.4:+29017911 | None:intergenic |
| GCACCTGATCTAAGAGGATA+TGG | 0.428528 | 8.4:-29017777 | MS.gene76453:CDS |
| AAAACCAACATCTTTGTCTC+CGG | 0.451884 | 8.4:+29016682 | None:intergenic |
| GAAACTATAAGAAAAGTATA+CGG | 0.452414 | 8.4:-29017200 | MS.gene76453:CDS |
| GTTGGAGATCTTGGAAAGTA+AGG | 0.455828 | 8.4:+29017236 | None:intergenic |
| TAGAGATAGTTTACGGATGA+AGG | 0.460572 | 8.4:+29016523 | None:intergenic |
| CTTGAAGTGGTTATACTAGA+TGG | 0.462250 | 8.4:-29016599 | MS.gene76453:CDS |
| CACCGGTGAAACCAGACTCT+TGG | 0.472500 | 8.4:+29016871 | None:intergenic |
| AGTATAACCACTTCAAGGTT+AGG | 0.474123 | 8.4:+29016605 | None:intergenic |
| TTCCAAGAGTCTGGTTTCAC+CGG | 0.475025 | 8.4:-29016873 | MS.gene76453:CDS |
| GCCAAGAATACCATATTTCT+GGG | 0.482112 | 8.4:+29017844 | None:intergenic |
| GGGCCAAGTAGTTGAGTTGG+TGG | 0.492922 | 8.4:+29017820 | None:intergenic |
| TCACCATATCCTCTTAGATC+AGG | 0.494603 | 8.4:+29017774 | None:intergenic |
| GTGATGAAGAGTGTAGGAAT+TGG | 0.497504 | 8.4:+29017731 | None:intergenic |
| CTTTCTCTTGATGTATATAG+TGG | 0.499966 | 8.4:+29016573 | None:intergenic |
| GTTCACCAAAATGATCAAGA+AGG | 0.500450 | 8.4:+29017688 | None:intergenic |
| CATCTAGTATAACCACTTCA+AGG | 0.501344 | 8.4:+29016600 | None:intergenic |
| TTTGGGCCAAGTAGTTGAGT+TGG | 0.505675 | 8.4:+29017817 | None:intergenic |
| TGAATGAAGGCATATGTAGT+AGG | 0.512881 | 8.4:-29017341 | MS.gene76453:intron |
| TATCAGGCCTAAATAGACTT+AGG | 0.515370 | 8.4:+29017288 | None:intergenic |
| ATAGTTTACGGATGAAGGAA+AGG | 0.519190 | 8.4:+29016528 | None:intergenic |
| GAAAGCTCAACAAGTTTCAG+AGG | 0.521146 | 8.4:-29016555 | MS.gene76453:CDS |
| CATATGTAGTAGGATCTGAC+TGG | 0.526683 | 8.4:-29017331 | MS.gene76453:CDS |
| CATGTGGCAGAACAAGGAAC+AGG | 0.531255 | 8.4:-29017894 | MS.gene76453:CDS |
| GTTATCTTTGATCCTTGCCA+TGG | 0.532855 | 8.4:+29016725 | None:intergenic |
| CAAGGTTAGGAACAAGACTC+TGG | 0.533494 | 8.4:+29016618 | None:intergenic |
| GCGCCACCAACTCAACTACT+TGG | 0.535864 | 8.4:-29017823 | MS.gene76453:CDS |
| TATAACAGCTCCTCCTGGCA+TGG | 0.539454 | 8.4:-29016976 | MS.gene76453:CDS |
| TCTTGTTCCTAACCTTGAAG+TGG | 0.541399 | 8.4:-29016612 | MS.gene76453:CDS |
| TTGCGTGGTTGGCAGGAACC+AGG | 0.547693 | 8.4:-29017077 | MS.gene76453:intron |
| AAATCAACAATCTCCATGCC+AGG | 0.548033 | 8.4:+29016963 | None:intergenic |
| TCTGACTGGGGAGCAAACAT+TGG | 0.557088 | 8.4:-29017317 | MS.gene76453:CDS |
| TTTAGGCCTGATAGAGTGAA+GGG | 0.566974 | 8.4:-29017278 | MS.gene76453:CDS |
| ATAAGAAAAGTATACGGAGA+TGG | 0.570679 | 8.4:-29017194 | MS.gene76453:CDS |
| ATTGCCGGAGACAAAGATGT+TGG | 0.571908 | 8.4:-29016686 | MS.gene76453:CDS |
| AATATACATGTGGCAGAACA+AGG | 0.572841 | 8.4:-29017900 | MS.gene76453:CDS |
| ACTGGGGAGCAAACATTGGA+TGG | 0.572887 | 8.4:-29017313 | MS.gene76453:CDS |
| GCTGTTGCACCTGATCTAAG+AGG | 0.588705 | 8.4:-29017783 | MS.gene76453:CDS |
| ATATGTAGTAGGATCTGACT+GGG | 0.595018 | 8.4:-29017330 | MS.gene76453:CDS |
| AAAGCTCTCTCTGCACGACC+TGG | 0.595328 | 8.4:+29017059 | None:intergenic |
| ATCCACGACGGCAAAACCGA+AGG | 0.595962 | 8.4:+29016930 | None:intergenic |
| GACGGCAAAACCGAAGGAGT+TGG | 0.599928 | 8.4:+29016936 | None:intergenic |
| ACTCAATGGTTACAGAAGCT+GGG | 0.603841 | 8.4:-29016759 | MS.gene76453:intron |
| CACCAAAGAATCAAAACCAA+CGG | 0.604850 | 8.4:-29017927 | MS.gene76453:CDS |
| ATTTAGGCCTGATAGAGTGA+AGG | 0.608580 | 8.4:-29017279 | MS.gene76453:CDS |
| GATAACCGAGGAAGAACTCA+TGG | 0.608940 | 8.4:-29016910 | MS.gene76453:CDS |
| ATATTTCTGGGAAACCATGA+AGG | 0.612668 | 8.4:+29017856 | None:intergenic |
| CCATGAAGGAGTAGAACTAG+TGG | 0.626025 | 8.4:+29017870 | None:intergenic |
| ATTCACTTCACTCATGAACA+TGG | 0.637224 | 8.4:+29017950 | None:intergenic |
| TTAGGCCTGATAGAGTGAAG+GGG | 0.646633 | 8.4:-29017277 | MS.gene76453:CDS |
| GAACTTCTAGCACCATGGCA+AGG | 0.648382 | 8.4:-29016737 | MS.gene76453:CDS |
| GATGAAGAGTGTAGGAATTG+GGG | 0.650623 | 8.4:+29017733 | None:intergenic |
| CAACGGAATAAATATACATG+TGG | 0.654586 | 8.4:-29017910 | MS.gene76453:CDS |
| GCGATAGTAATTGAATGCAC+CGG | 0.666695 | 8.4:+29016854 | None:intergenic |
| TACACTCTTCATCACATCGT+AGG | 0.670287 | 8.4:-29017723 | MS.gene76453:CDS |
| TACGATGTGATGAAGAGTGT+AGG | 0.674600 | 8.4:+29017725 | None:intergenic |
| TTTGCCGTCGTGGATAACCG+AGG | 0.689324 | 8.4:-29016922 | MS.gene76453:CDS |
| GCTGGGAACTTCTAGCACCA+TGG | 0.691193 | 8.4:-29016742 | MS.gene76453:CDS |
| TCTTCCTCGGTTATCCACGA+CGG | 0.703931 | 8.4:+29016918 | None:intergenic |
| ATTCAATTACTATCGCGCAA+TGG | 0.705978 | 8.4:-29016847 | MS.gene76453:CDS |
| TCAACAATCTCCATGCCAGG+AGG | 0.721559 | 8.4:+29016966 | None:intergenic |
| TATGTAGTAGGATCTGACTG+GGG | 0.804727 | 8.4:-29017329 | MS.gene76453:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACTAAACAAAAAAACAT+AGG | + | chr8.4:29017663-29017682 | None:intergenic | 15.0% |
| !! | AAATTAGGAAATTAGGAAAA+AGG | + | chr8.4:29017087-29017106 | None:intergenic | 20.0% |
| !! | AATCAATAAAATTGACATAG+AGG | + | chr8.4:29016915-29016934 | None:intergenic | 20.0% |
| !! | ACAAAAAAACATAGGAAAAA+TGG | + | chr8.4:29017655-29017674 | None:intergenic | 20.0% |
| !! | AGAGAATAAATTAGGAAATT+AGG | + | chr8.4:29017094-29017113 | None:intergenic | 20.0% |
| !! | GAAATATAATTGTACACAAT+TGG | - | chr8.4:29017049-29017068 | MS.gene76453:CDS | 20.0% |
| !!! | GAAACTATAAGAAAAGTATA+CGG | - | chr8.4:29017265-29017284 | MS.gene76453:CDS | 20.0% |
| ! | AAACGATAGATGTAAACTTA+AGG | + | chr8.4:29016975-29016994 | None:intergenic | 25.0% |
| ! | AACTTTATCAAGCCATAAAA+GGG | + | chr8.4:29017327-29017346 | None:intergenic | 25.0% |
| ! | TAACTTTATCAAGCCATAAA+AGG | + | chr8.4:29017328-29017347 | None:intergenic | 25.0% |
| !! | TAATTTTCATTGAAATTGCG+TGG | - | chr8.4:29017373-29017392 | MS.gene76453:intron | 25.0% |
| !!! | AAAGATGTTGGTTTTCAAAA+TGG | - | chr8.4:29017791-29017810 | MS.gene76453:CDS | 25.0% |
| !!! | AAGATGTTGGTTTTCAAAAT+GGG | - | chr8.4:29017792-29017811 | MS.gene76453:CDS | 25.0% |
| AACCATTGAGTGTGAATAAA+TGG | + | chr8.4:29017697-29017716 | None:intergenic | 30.0% | |
| AAGTTTACATCTATCGTTTC+AGG | - | chr8.4:29016976-29016995 | MS.gene76453:CDS | 30.0% | |
| ATAGGAAAAATGGTACTTAC+AGG | + | chr8.4:29017645-29017664 | None:intergenic | 30.0% | |
| ATCCATTTATTCACACTCAA+TGG | - | chr8.4:29017692-29017711 | MS.gene76453:CDS | 30.0% | |
| ATCTACAACATGAAAAAACC+TGG | + | chr8.4:29016844-29016863 | None:intergenic | 30.0% | |
| CAACGGAATAAATATACATG+TGG | - | chr8.4:29016555-29016574 | MS.gene76453:CDS | 30.0% | |
| CACATGTATATTTATTCCGT+TGG | + | chr8.4:29016557-29016576 | None:intergenic | 30.0% | |
| CGGTATAGAGAGAATAAATT+AGG | + | chr8.4:29017102-29017121 | None:intergenic | 30.0% | |
| CTTTCTCTTGATGTATATAG+TGG | + | chr8.4:29017895-29017914 | None:intergenic | 30.0% | |
| TAAAATTGACATAGAGGTCT+TGG | + | chr8.4:29016909-29016928 | None:intergenic | 30.0% | |
| TACATATGCCTTCATTCAAA+CGG | + | chr8.4:29017122-29017141 | None:intergenic | 30.0% | |
| TAGATGTAAACTTAAGGTCT+TGG | + | chr8.4:29016969-29016988 | None:intergenic | 30.0% | |
| TAGGAAATTAGGAAAAAGGA+TGG | + | chr8.4:29017083-29017102 | None:intergenic | 30.0% | |
| ! | AACAGCATGATAACCATTTT+GGG | + | chr8.4:29016668-29016687 | None:intergenic | 30.0% |
| ! | ATAAGAAAAGTATACGGAGA+TGG | - | chr8.4:29017271-29017290 | MS.gene76453:CDS | 30.0% |
| ! | ATCGTTTCAGGTCTTAATTT+AGG | - | chr8.4:29016988-29017007 | MS.gene76453:CDS | 30.0% |
| !! | AGAAGTTTTTGCTGATTACA+TGG | - | chr8.4:29017458-29017477 | MS.gene76453:intron | 30.0% |
| !! | GTGTTATTGACTCAAACATA+TGG | + | chr8.4:29016947-29016966 | None:intergenic | 30.0% |
| !! | GTTTCAACAGTTTTATCTGT+TGG | + | chr8.4:29017250-29017269 | None:intergenic | 30.0% |
| !! | TGATCATTTTGGTGAACAAA+AGG | - | chr8.4:29016783-29016802 | MS.gene76453:intron | 30.0% |
| !!! | AGATGTTGGTTTTCAAAATG+GGG | - | chr8.4:29017793-29017812 | MS.gene76453:CDS | 30.0% |
| AAAAACCATGAGTTCTTCCT+CGG | + | chr8.4:29017563-29017582 | None:intergenic | 35.0% | |
| AAAACCAACATCTTTGTCTC+CGG | + | chr8.4:29017786-29017805 | None:intergenic | 35.0% | |
| AAGGGTAATCTACCTGAAAT+TGG | + | chr8.4:29017309-29017328 | None:intergenic | 35.0% | |
| AATATACATGTGGCAGAACA+AGG | - | chr8.4:29016565-29016584 | MS.gene76453:CDS | 35.0% | |
| AATGATCAAGAAGGCCAATA+AGG | + | chr8.4:29016771-29016790 | None:intergenic | 35.0% | |
| AGTATAACCACTTCAAGGTT+AGG | + | chr8.4:29017863-29017882 | None:intergenic | 35.0% | |
| ATAGTTTACGGATGAAGGAA+AGG | + | chr8.4:29017940-29017959 | None:intergenic | 35.0% | |
| ATATGTAGTAGGATCTGACT+GGG | - | chr8.4:29017135-29017154 | MS.gene76453:intron | 35.0% | |
| ATTCAATTACTATCGCGCAA+TGG | - | chr8.4:29017618-29017637 | MS.gene76453:intron | 35.0% | |
| CACCAAAGAATCAAAACCAA+CGG | - | chr8.4:29016538-29016557 | MS.gene76453:CDS | 35.0% | |
| CATCTAGTATAACCACTTCA+AGG | + | chr8.4:29017868-29017887 | None:intergenic | 35.0% | |
| CTTGAAGTGGTTATACTAGA+TGG | - | chr8.4:29017866-29017885 | MS.gene76453:CDS | 35.0% | |
| GCCAAGAATACCATATTTCT+GGG | + | chr8.4:29016624-29016643 | None:intergenic | 35.0% | |
| GTTCACCAAAATGATCAAGA+AGG | + | chr8.4:29016780-29016799 | None:intergenic | 35.0% | |
| GTTCCAACTAAGTTTATTGC+CGG | - | chr8.4:29017764-29017783 | MS.gene76453:CDS | 35.0% | |
| TACAACATGAAAAAACCTGG+AGG | + | chr8.4:29016841-29016860 | None:intergenic | 35.0% | |
| TAGAGATAGTTTACGGATGA+AGG | + | chr8.4:29017945-29017964 | None:intergenic | 35.0% | |
| TATCAGGCCTAAATAGACTT+AGG | + | chr8.4:29017180-29017199 | None:intergenic | 35.0% | |
| TCCCAGAAATATGGTATTCT+TGG | - | chr8.4:29016620-29016639 | MS.gene76453:CDS | 35.0% | |
| TCTCTATACCGTTTGAATGA+AGG | - | chr8.4:29017111-29017130 | MS.gene76453:intron | 35.0% | |
| TTCATGGTTTCCCAGAAATA+TGG | - | chr8.4:29016611-29016630 | MS.gene76453:CDS | 35.0% | |
| TTTCATTGAAATTGCGTGGT+TGG | - | chr8.4:29017377-29017396 | MS.gene76453:intron | 35.0% | |
| ! | CAACAGCATGATAACCATTT+TGG | + | chr8.4:29016669-29016688 | None:intergenic | 35.0% |
| ! | GGTACAAAAGATTTTGTGGA+AGG | - | chr8.4:29017815-29017834 | MS.gene76453:CDS | 35.0% |
| ! | GTTTTATCTGTTGGAGATCT+TGG | + | chr8.4:29017241-29017260 | None:intergenic | 35.0% |
| ! | TCAGGTAGATTACCCTTTTA+TGG | - | chr8.4:29017312-29017331 | MS.gene76453:CDS | 35.0% |
| ! | TGAATGAAGGCATATGTAGT+AGG | - | chr8.4:29017124-29017143 | MS.gene76453:intron | 35.0% |
| ! | TGATGAAGAGTGTAGGAATT+GGG | + | chr8.4:29016736-29016755 | None:intergenic | 35.0% |
| !! | ATATTTCTGGGAAACCATGA+AGG | + | chr8.4:29016612-29016631 | None:intergenic | 35.0% |
| !!! | ATTGGCCTTCTTGATCATTT+TGG | - | chr8.4:29016772-29016791 | MS.gene76453:intron | 35.0% |
| !!! | GATGTTGGTTTTCAAAATGG+GGG | - | chr8.4:29017794-29017813 | MS.gene76453:CDS | 35.0% |
| !!! | TTCCGTTGGTTTTGATTCTT+TGG | + | chr8.4:29016543-29016562 | None:intergenic | 35.0% |
| AAATCAACAATCTCCATGCC+AGG | + | chr8.4:29017505-29017524 | None:intergenic | 40.0% | |
| ACTCAATGGTTACAGAAGCT+GGG | - | chr8.4:29017706-29017725 | MS.gene76453:CDS | 40.0% | |
| AGCTCATGTTTGCCAATTTC+AGG | - | chr8.4:29017294-29017313 | MS.gene76453:CDS | 40.0% | |
| ATTTAGGCCTGATAGAGTGA+AGG | - | chr8.4:29017186-29017205 | MS.gene76453:CDS | 40.0% | |
| CATATGTAGTAGGATCTGAC+TGG | - | chr8.4:29017134-29017153 | MS.gene76453:intron | 40.0% | |
| CGCCAAGAATACCATATTTC+TGG | + | chr8.4:29016625-29016644 | None:intergenic | 40.0% | |
| GAAAGCTCAACAAGTTTCAG+AGG | - | chr8.4:29017910-29017929 | MS.gene76453:CDS | 40.0% | |
| GATGGCACCTAAGTCTATTT+AGG | - | chr8.4:29017170-29017189 | MS.gene76453:intron | 40.0% | |
| GCAACATGAAAATGTCACGT+TGG | + | chr8.4:29016885-29016904 | None:intergenic | 40.0% | |
| GCGATAGTAATTGAATGCAC+CGG | + | chr8.4:29017614-29017633 | None:intergenic | 40.0% | |
| GTTATCTTTGATCCTTGCCA+TGG | + | chr8.4:29017743-29017762 | None:intergenic | 40.0% | |
| GTTGGAGATCTTGGAAAGTA+AGG | + | chr8.4:29017232-29017251 | None:intergenic | 40.0% | |
| TACACTCTTCATCACATCGT+AGG | - | chr8.4:29016742-29016761 | MS.gene76453:CDS | 40.0% | |
| TATGTAGTAGGATCTGACTG+GGG | - | chr8.4:29017136-29017155 | MS.gene76453:intron | 40.0% | |
| TCACCATATCCTCTTAGATC+AGG | + | chr8.4:29016694-29016713 | None:intergenic | 40.0% | |
| TCTCCGGCAATAAACTTAGT+TGG | + | chr8.4:29017770-29017789 | None:intergenic | 40.0% | |
| TCTTGTTCCTAACCTTGAAG+TGG | - | chr8.4:29017853-29017872 | MS.gene76453:CDS | 40.0% | |
| TGATTTCTTGCCAACTCCTT+CGG | - | chr8.4:29017519-29017538 | MS.gene76453:intron | 40.0% | |
| TGTCACGTTGGACATGAATT+TGG | + | chr8.4:29016873-29016892 | None:intergenic | 40.0% | |
| TTTAGGCCTGATAGAGTGAA+GGG | - | chr8.4:29017187-29017206 | MS.gene76453:CDS | 40.0% | |
| ! | CATTTTATAACAGCTCCTCC+TGG | - | chr8.4:29017484-29017503 | MS.gene76453:intron | 40.0% |
| ! | GATGAAGAGTGTAGGAATTG+GGG | + | chr8.4:29016735-29016754 | None:intergenic | 40.0% |
| ! | GTGATGAAGAGTGTAGGAAT+TGG | + | chr8.4:29016737-29016756 | None:intergenic | 40.0% |
| ! | TACGATGTGATGAAGAGTGT+AGG | + | chr8.4:29016743-29016762 | None:intergenic | 40.0% |
| !! | TGGGGGTACAAAAGATTTTG+TGG | - | chr8.4:29017811-29017830 | MS.gene76453:CDS | 40.0% |
| ACAAACCCCTTCACTCTATC+AGG | + | chr8.4:29017196-29017215 | None:intergenic | 45.0% | |
| ATTGAAATTGCGTGGTTGGC+AGG | - | chr8.4:29017381-29017400 | MS.gene76453:intron | 45.0% | |
| ATTGCCGGAGACAAAGATGT+TGG | - | chr8.4:29017779-29017798 | MS.gene76453:CDS | 45.0% | |
| CAAGGTTAGGAACAAGACTC+TGG | + | chr8.4:29017850-29017869 | None:intergenic | 45.0% | |
| CACATCGTAGGTGACCTTAT+TGG | - | chr8.4:29016754-29016773 | MS.gene76453:intron | 45.0% | |
| CACGTTGGACATGAATTTGG+AGG | + | chr8.4:29016870-29016889 | None:intergenic | 45.0% | |
| CACTCAATGGTTACAGAAGC+TGG | - | chr8.4:29017705-29017724 | MS.gene76453:CDS | 45.0% | |
| CCATGAAGGAGTAGAACTAG+TGG | + | chr8.4:29016598-29016617 | None:intergenic | 45.0% | |
| CTCAACTACTTGGCCCAAAA+TGG | - | chr8.4:29016652-29016671 | MS.gene76453:CDS | 45.0% | |
| GATAACCGAGGAAGAACTCA+TGG | - | chr8.4:29017555-29017574 | MS.gene76453:intron | 45.0% | |
| GCACCTGATCTAAGAGGATA+TGG | - | chr8.4:29016688-29016707 | MS.gene76453:CDS | 45.0% | |
| GCAGACAAATTCCAAGAGTC+TGG | - | chr8.4:29017583-29017602 | MS.gene76453:intron | 45.0% | |
| TTAGGCCTGATAGAGTGAAG+GGG | - | chr8.4:29017188-29017207 | MS.gene76453:CDS | 45.0% | |
| TTTGGGCCAAGTAGTTGAGT+TGG | + | chr8.4:29016651-29016670 | None:intergenic | 45.0% | |
| ! | GTGTAGGAATTGGGGTTGAT+AGG | + | chr8.4:29016727-29016746 | None:intergenic | 45.0% |
| ! | TTCCAAGAGTCTGGTTTCAC+CGG | - | chr8.4:29017592-29017611 | MS.gene76453:intron | 45.0% |
| !! | CCACTAGTTCTACTCCTTCA+TGG | - | chr8.4:29016595-29016614 | MS.gene76453:CDS | 45.0% |
| ACCAAATCTCTTGAGCCTCC+AGG | - | chr8.4:29016823-29016842 | MS.gene76453:intron | 50.0% | |
| ACCTGGAGGCTCAAGAGATT+TGG | + | chr8.4:29016827-29016846 | None:intergenic | 50.0% | |
| ACTGGGGAGCAAACATTGGA+TGG | - | chr8.4:29017152-29017171 | MS.gene76453:intron | 50.0% | |
| CATGTGGCAGAACAAGGAAC+AGG | - | chr8.4:29016571-29016590 | MS.gene76453:CDS | 50.0% | |
| GAACTTCTAGCACCATGGCA+AGG | - | chr8.4:29017728-29017747 | MS.gene76453:CDS | 50.0% | |
| GCTGTTGCACCTGATCTAAG+AGG | - | chr8.4:29016682-29016701 | MS.gene76453:CDS | 50.0% | |
| TATAACAGCTCCTCCTGGCA+TGG | - | chr8.4:29017489-29017508 | MS.gene76453:intron | 50.0% | |
| TCAACAATCTCCATGCCAGG+AGG | + | chr8.4:29017502-29017521 | None:intergenic | 50.0% | |
| TCTGACTGGGGAGCAAACAT+TGG | - | chr8.4:29017148-29017167 | MS.gene76453:intron | 50.0% | |
| TCTTCCTCGGTTATCCACGA+CGG | + | chr8.4:29017550-29017569 | None:intergenic | 50.0% | |
| AAAGCTCTCTCTGCACGACC+TGG | + | chr8.4:29017409-29017428 | None:intergenic | 55.0% | |
| ATCCACGACGGCAAAACCGA+AGG | + | chr8.4:29017538-29017557 | None:intergenic | 55.0% | |
| CACCGGTGAAACCAGACTCT+TGG | + | chr8.4:29017597-29017616 | None:intergenic | 55.0% | |
| GACGGCAAAACCGAAGGAGT+TGG | + | chr8.4:29017532-29017551 | None:intergenic | 55.0% | |
| GCGCCACCAACTCAACTACT+TGG | - | chr8.4:29016642-29016661 | MS.gene76453:CDS | 55.0% | |
| GCTGGGAACTTCTAGCACCA+TGG | - | chr8.4:29017723-29017742 | MS.gene76453:CDS | 55.0% | |
| TTTGCCGTCGTGGATAACCG+AGG | - | chr8.4:29017543-29017562 | MS.gene76453:intron | 55.0% | |
| !! | GGGCCAAGTAGTTGAGTTGG+TGG | + | chr8.4:29016648-29016667 | None:intergenic | 55.0% |
| TTGCGTGGTTGGCAGGAACC+AGG | - | chr8.4:29017388-29017407 | MS.gene76453:intron | 60.0% | |
| ! | CTCCTTCGGTTTTGCCGTCG+TGG | - | chr8.4:29017533-29017552 | MS.gene76453:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 29016517 | 29017970 | 29016517 | ID=MS.gene76453 |
| chr8.4 | mRNA | 29016517 | 29017970 | 29016517 | ID=MS.gene76453.t1;Parent=MS.gene76453 |
| chr8.4 | exon | 29017683 | 29017970 | 29017683 | ID=MS.gene76453.t1.exon1;Parent=MS.gene76453.t1 |
| chr8.4 | CDS | 29017683 | 29017970 | 29017683 | ID=cds.MS.gene76453.t1;Parent=MS.gene76453.t1 |
| chr8.4 | exon | 29017172 | 29017354 | 29017172 | ID=MS.gene76453.t1.exon2;Parent=MS.gene76453.t1 |
| chr8.4 | CDS | 29017172 | 29017354 | 29017172 | ID=cds.MS.gene76453.t1;Parent=MS.gene76453.t1 |
| chr8.4 | exon | 29016843 | 29017084 | 29016843 | ID=MS.gene76453.t1.exon3;Parent=MS.gene76453.t1 |
| chr8.4 | CDS | 29016843 | 29017084 | 29016843 | ID=cds.MS.gene76453.t1;Parent=MS.gene76453.t1 |
| chr8.4 | exon | 29016517 | 29016766 | 29016517 | ID=MS.gene76453.t1.exon4;Parent=MS.gene76453.t1 |
| chr8.4 | CDS | 29016517 | 29016766 | 29016517 | ID=cds.MS.gene76453.t1;Parent=MS.gene76453.t1 |
| Gene Sequence |
| Protein sequence |
