AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0985260


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 4 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGGATTTGAGGAATATAA+TGG 0.331678 6_4:+31471725 Msa0985260:intron
AAGAGAACACAGTGCAAAGC+AGG 0.462698 6_4:+31471753 Msa0985260:CDS
AGAGTGCATGGAAATTGCTT+AGG 0.486728 6_4:-31471825 None:intergenic
ATCAATTCACAATTATATAG+CGG 0.637629 6_4:+31471783 Msa0985260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTTTTAAAATATTATCAT+TGG + chr6_4:31471446-31471465 Msa0985260:intron 10.0%
!! AATATCTTTCATTAAAAAAA+TGG - chr6_4:31471676-31471695 None:intergenic 10.0%
!!! ATCTTTTAAAATATTATCAT+TGG + chr6_4:31471446-31471465 Msa0985260:intron 10.0%
!! AATATCTTTCATTAAAAAAA+TGG - chr6_4:31471676-31471695 None:intergenic 10.0%
!! TGTTAAACTAAAACTTTAAA+AGG - chr6_4:31471412-31471431 None:intergenic 15.0%
!!! TTGTTTGATTTAAAATTTCA+AGG + chr6_4:31471595-31471614 Msa0985260:intron 15.0%
!!! TGTTTGATTTAAAATTTCAA+GGG + chr6_4:31471596-31471615 Msa0985260:intron 15.0%
!! TGTTAAACTAAAACTTTAAA+AGG - chr6_4:31471412-31471431 None:intergenic 15.0%
!!! TTGTTTGATTTAAAATTTCA+AGG + chr6_4:31471595-31471614 Msa0985260:intron 15.0%
!!! TGTTTGATTTAAAATTTCAA+GGG + chr6_4:31471596-31471615 Msa0985260:intron 15.0%
!!! TTTTCCAAATAGGTATTTAT+CGG - chr6_4:31471206-31471225 None:intergenic 20.0%
!! AATATGATCATAATCTATAG+TGG - chr6_4:31471523-31471542 None:intergenic 20.0%
!! TTATAGAATTTAGCTAGAAA+AGG + chr6_4:31471541-31471560 Msa0985260:intron 20.0%
!! ATCAATTCACAATTATATAG+CGG + chr6_4:31471783-31471802 Msa0985260:CDS 20.0%
!!! TTTTCCAAATAGGTATTTAT+CGG - chr6_4:31471206-31471225 None:intergenic 20.0%
!! AATATGATCATAATCTATAG+TGG - chr6_4:31471523-31471542 None:intergenic 20.0%
!! TTATAGAATTTAGCTAGAAA+AGG + chr6_4:31471541-31471560 Msa0985260:intron 20.0%
!! ATCAATTCACAATTATATAG+CGG + chr6_4:31471783-31471802 Msa0985260:CDS 20.0%
! TTTCCAAATAGGTATTTATC+GGG - chr6_4:31471205-31471224 None:intergenic 25.0%
!!! TACCTATTTGGAAAAGTATT+TGG + chr6_4:31471211-31471230 Msa0985260:intron 25.0%
!! TACCAAATACTTTTCCAAAT+AGG - chr6_4:31471216-31471235 None:intergenic 25.0%
!! TTATAGAGCAACTTTTCAAA+AGG - chr6_4:31471351-31471370 None:intergenic 25.0%
! TCTAGCTAAAACTGTTTATT+AGG - chr6_4:31471631-31471650 None:intergenic 25.0%
! GCTAGATTGTATAATGTTTA+AGG + chr6_4:31471645-31471664 Msa0985260:intron 25.0%
!! ATATACTTTTGTAGGATTTG+AGG + chr6_4:31471715-31471734 Msa0985260:intron 25.0%
!! AAATGCGATTTTGTCATATA+CGG + chr6_4:31472010-31472029 Msa0985260:three_prime_UTR 25.0%
! TTTCCAAATAGGTATTTATC+GGG - chr6_4:31471205-31471224 None:intergenic 25.0%
!!! TACCTATTTGGAAAAGTATT+TGG + chr6_4:31471211-31471230 Msa0985260:intron 25.0%
!! TACCAAATACTTTTCCAAAT+AGG - chr6_4:31471216-31471235 None:intergenic 25.0%
!! TTATAGAGCAACTTTTCAAA+AGG - chr6_4:31471351-31471370 None:intergenic 25.0%
! TCTAGCTAAAACTGTTTATT+AGG - chr6_4:31471631-31471650 None:intergenic 25.0%
! GCTAGATTGTATAATGTTTA+AGG + chr6_4:31471645-31471664 Msa0985260:intron 25.0%
!! ATATACTTTTGTAGGATTTG+AGG + chr6_4:31471715-31471734 Msa0985260:intron 25.0%
!! AAATGCGATTTTGTCATATA+CGG + chr6_4:31472010-31472029 Msa0985260:three_prime_UTR 25.0%
TAACCCGATAAATACCTATT+TGG + chr6_4:31471199-31471218 Msa0985260:intron 30.0%
! AAAGGCACAACACTTTATTT+AGG - chr6_4:31471333-31471352 None:intergenic 30.0%
AATAAAGATGAGAATGCAGA+AGG + chr6_4:31471480-31471499 Msa0985260:intron 30.0%
! ATGTGTGCATATACTTTTGT+AGG + chr6_4:31471707-31471726 Msa0985260:intron 30.0%
GTAGGATTTGAGGAATATAA+TGG + chr6_4:31471725-31471744 Msa0985260:intron 30.0%
ATACTATCATAAACCACCAT+GGG - chr6_4:31471088-31471107 None:intergenic 30.0%
TAACCCGATAAATACCTATT+TGG + chr6_4:31471199-31471218 Msa0985260:intron 30.0%
! AAAGGCACAACACTTTATTT+AGG - chr6_4:31471333-31471352 None:intergenic 30.0%
AATAAAGATGAGAATGCAGA+AGG + chr6_4:31471480-31471499 Msa0985260:intron 30.0%
! ATGTGTGCATATACTTTTGT+AGG + chr6_4:31471707-31471726 Msa0985260:intron 30.0%
GTAGGATTTGAGGAATATAA+TGG + chr6_4:31471725-31471744 Msa0985260:intron 30.0%
TGGTTTATGATAGTATCCCA+AGG + chr6_4:31471092-31471111 Msa0985260:intron 35.0%
! TTTTTGTGTGTGTTGTACGT+AGG - chr6_4:31471128-31471147 None:intergenic 35.0%
AAGTTGCTCTATAAACCTTG+TGG + chr6_4:31471357-31471376 Msa0985260:intron 35.0%
TCTATAAACCTTGTGGAAGT+AGG + chr6_4:31471364-31471383 Msa0985260:intron 35.0%
CATTTAAACCTACTTCCACA+AGG - chr6_4:31471375-31471394 None:intergenic 35.0%
! ATTTTCTGAATCAGAGTGCA+TGG - chr6_4:31471840-31471859 None:intergenic 35.0%
GATACTATCATAAACCACCA+TGG - chr6_4:31471089-31471108 None:intergenic 35.0%
TGGTTTATGATAGTATCCCA+AGG + chr6_4:31471092-31471111 Msa0985260:intron 35.0%
! TTTTTGTGTGTGTTGTACGT+AGG - chr6_4:31471128-31471147 None:intergenic 35.0%
AAGTTGCTCTATAAACCTTG+TGG + chr6_4:31471357-31471376 Msa0985260:intron 35.0%
TCTATAAACCTTGTGGAAGT+AGG + chr6_4:31471364-31471383 Msa0985260:intron 35.0%
CATTTAAACCTACTTCCACA+AGG - chr6_4:31471375-31471394 None:intergenic 35.0%
! ATTTTCTGAATCAGAGTGCA+TGG - chr6_4:31471840-31471859 None:intergenic 35.0%
! CGTAGGAAGAAGTATACCTT+GGG - chr6_4:31471111-31471130 None:intergenic 40.0%
! ACGTAGGAAGAAGTATACCT+TGG - chr6_4:31471112-31471131 None:intergenic 40.0%
ATCTATAGTGGTTGACCTGA+TGG - chr6_4:31471511-31471530 None:intergenic 40.0%
AGAGTGCATGGAAATTGCTT+AGG - chr6_4:31471828-31471847 None:intergenic 40.0%
! CGTAGGAAGAAGTATACCTT+GGG - chr6_4:31471111-31471130 None:intergenic 40.0%
! ACGTAGGAAGAAGTATACCT+TGG - chr6_4:31471112-31471131 None:intergenic 40.0%
ATCTATAGTGGTTGACCTGA+TGG - chr6_4:31471511-31471530 None:intergenic 40.0%
AGAGTGCATGGAAATTGCTT+AGG - chr6_4:31471828-31471847 None:intergenic 40.0%
AAGAGAACACAGTGCAAAGC+AGG + chr6_4:31471753-31471772 Msa0985260:CDS 45.0%
AAGAGAACACAGTGCAAAGC+AGG + chr6_4:31471753-31471772 Msa0985260:CDS 45.0%
ATGCAGAAGGTGAGTCCATC+AGG + chr6_4:31471493-31471512 Msa0985260:intron 50.0%
ATGCAGAAGGTGAGTCCATC+AGG + chr6_4:31471493-31471512 Msa0985260:intron 50.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 31471088 31472053 31471088 ID=Msa0985260;Name=Msa0985260
chr6_4 mRNA 31471088 31472053 31471088 ID=Msa0985260-mRNA-1;Parent=Msa0985260;Name=Msa0985260-mRNA-1;_AED=0.35;_eAED=0.36;_QI=0|0|0.5|1|0|0.5|2|124|68
chr6_4 exon 31471088 31471093 31471088 ID=Msa0985260-mRNA-1:exon:8439;Parent=Msa0985260-mRNA-1
chr6_4 exon 31471729 31472053 31471729 ID=Msa0985260-mRNA-1:exon:8440;Parent=Msa0985260-mRNA-1
chr6_4 CDS 31471088 31471093 31471088 ID=Msa0985260-mRNA-1:cds;Parent=Msa0985260-mRNA-1
chr6_4 CDS 31471729 31471929 31471729 ID=Msa0985260-mRNA-1:cds;Parent=Msa0985260-mRNA-1
chr6_4 three_prime_UTR 31471930 31472053 31471930 ID=Msa0985260-mRNA-1:three_prime_utr;Parent=Msa0985260-mRNA-1
Gene Sequence

>Msa0985260

ATGGTGGATTTGAGGAATATAATGGAAATAAAGAGAACACAGTGCAAAGCAGGAAGATGCATCAATTCACAATTATATAGCGGAAAAAGAAAAAAAAAACAACCTAAGCAATTTCCATGCACTCTGATTCAGAAAATGTCACTTCTTTTTTCTTCATTTGTTTTGTGTTATGAGATTATTATTCTCATTATTGTGCTTGATGTTTGA

Protein sequence

>Msa0985260

MVDLRNIMEIKRTQCKAGRCINSQLYSGKRKKKQPKQFPCTLIQKMSLLFSSFVLCYEIIILIIVLDV*