AlfalfaGEDB | Msa1228290

M. sativa cultivar zhongmu-4 / Msa1228290

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0007005 GO:0008150 GO:0008152 GO:0008380 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0032502 GO:0034622 GO:0034641 GO:0042254 GO:0042255 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0065003 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363
PFAM: PF02536.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1228290	XP_013443746.1	94.336	565	31	1	16	580	1	564	0.0	1093

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1228290	sp\|Q9M219\|MTEFH_ARATH	46.507	544	284	3	36	578	17	554	1.51e-176	513

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1228290	A0A072TKN3	94.336	565	31	1	16	580	1	564	0.0	1093

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TFs/TRs:

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Gene ID	Type	Classification
Msa1228290	TR	mTERF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0008410	Msa1228290	0.819694	1.015167e-52	-8.615850e-47
Msa0027390	Msa1228290	0.822602	2.169749e-53	-8.615850e-47
Msa0032370	Msa1228290	0.825518	4.488337e-54	-8.615850e-47
Msa0032840	Msa1228290	-0.802562	5.288280e-49	-8.615850e-47
Msa0039250	Msa1228290	0.804654	1.946983e-49	-8.615850e-47
Msa0044020	Msa1228290	0.821800	3.329182e-53	-8.615850e-47
Msa0048000	Msa1228290	0.800656	1.301082e-48	-8.615850e-47
Msa0057710	Msa1228290	0.806179	9.329245e-50	-8.615850e-47
Msa0073670	Msa1228290	0.804175	2.450594e-49	-8.615850e-47
Msa0110970	Msa1228290	0.813703	2.238077e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1228290	MtrunA17_Chr8g0335121	94.336	565	31	1	16	580	1	564	0.0	1093
Msa1228290	MtrunA17_Chr7g0249711	55.856	555	230	6	24	572	31	576	0.0	635
Msa1228290	MtrunA17_Chr5g0438661	25.568	528	372	9	45	568	97	607	1.61e-52	189
Msa1228290	MtrunA17_Chr7g0258521	42.667	150	75	3	38	180	29	174	1.32e-24	107
Msa1228290	MtrunA17_Chr7g0258521	25.926	162	117	3	410	570	302	461	7.07e-14	74.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1228290	AT3G60400.1	46.507	544	284	3	36	578	17	554	1.54e-177	513
Msa1228290	AT4G19650.2	26.476	525	351	8	54	568	85	584	1.89e-51	186
Msa1228290	AT4G19650.1	25.447	503	340	8	76	568	90	567	5.39e-44	165
Msa1228290	AT5G06810.1	25.095	526	351	13	56	567	78	574	2.65e-39	152
Msa1228290	AT5G06811.1	24.014	583	393	12	15	567	8	570	1.24e-38	150
Msa1228290	AT5G45113.1	22.803	421	295	11	157	568	7	406	3.09e-21	97.1

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Find 138 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGGTTCTGAGATAAA+GGG	0.465819	8_2:-3764758	Msa1228290:CDS
AAAAGTGTCCAAAAGCTCTA+AGG	0.419374	8_2:+3763797	None:intergenic
AAAATTCAAACTCATTGATG+GGG	0.586544	8_2:+3764954	None:intergenic
AAACATTTGATTCTTGAGTA+TGG	0.263491	8_2:-3764458	Msa1228290:CDS
AAACTATTCCATTGCAAGCA+TGG	0.484064	8_2:-3763685	Msa1228290:CDS
AAAGAGTCATATAGATTCAA+AGG	0.514350	8_2:+3763316	None:intergenic
AAAGATTAATTTCCTATGTC+AGG	0.406425	8_2:-3763832	Msa1228290:CDS
AAGAACTATGCTCATGCGTT+GGG	0.457899	8_2:-3764254	Msa1228290:CDS
AAGGGATGGATCTTGTGGAA+TGG	0.590760	8_2:+3765790	None:intergenic
AAGTAGTGGCTATAGCGCAG+TGG	0.712669	8_2:-3763055	Msa1228290:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTCTAATCTCATTTCAAAGT+TGG	-	chr8_2:3762968-3762987	Msa1228290:three_prime_UTR	30.0%
TTTGTGAATTTGGATTTCCT+TGG	-	chr8_2:3763866-3763885	Msa1228290:CDS	30.0%
TTGTGAATTTGGATTTCCTT+GGG	-	chr8_2:3763867-3763886	Msa1228290:CDS	30.0%
ATGTTTGGTATGATGTTTGT+AGG	-	chr8_2:3764145-3764164	Msa1228290:CDS	30.0%
TTATGATTTGAGTGATGGTA+AGG	-	chr8_2:3764182-3764201	Msa1228290:CDS	30.0%
TATGATTTGAGTGATGGTAA+GGG	-	chr8_2:3764183-3764202	Msa1228290:CDS	30.0%
CATGATAAAGGTTATCAAAG+TGG	-	chr8_2:3764591-3764610	Msa1228290:CDS	30.0%
TTTGATTGCCTTCTACATTT+AGG	-	chr8_2:3764759-3764778	Msa1228290:CDS	30.0%
TTGATTGCCTTCTACATTTA+GGG	-	chr8_2:3764760-3764779	Msa1228290:CDS	30.0%
ATTCAATCCCTAAATGTAGA+AGG	+	chr8_2:3764770-3764789	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	3762696	3766018	3762696	ID=Msa1228290;Name=Msa1228290
chr8_2	mRNA	3762696	3766018	3762696	ID=Msa1228290-mRNA-1;Parent=Msa1228290;Name=Msa1228290-mRNA-1;_AED=0.20;_eAED=0.20;_QI=15\|1\|1\|1\|1\|1\|2\|882\|580
chr8_2	exon	3762696	3764982	3762696	ID=Msa1228290-mRNA-1:exon:1170;Parent=Msa1228290-mRNA-1
chr8_2	exon	3765666	3766018	3765666	ID=Msa1228290-mRNA-1:exon:1169;Parent=Msa1228290-mRNA-1
chr8_2	five_prime_UTR	3766004	3766018	3766004	ID=Msa1228290-mRNA-1:five_prime_utr;Parent=Msa1228290-mRNA-1
chr8_2	CDS	3765666	3766003	3765666	ID=Msa1228290-mRNA-1:cds;Parent=Msa1228290-mRNA-1
chr8_2	CDS	3763578	3764982	3763578	ID=Msa1228290-mRNA-1:cds;Parent=Msa1228290-mRNA-1
chr8_2	three_prime_UTR	3762696	3763577	3762696	ID=Msa1228290-mRNA-1:three_prime_utr;Parent=Msa1228290-mRNA-1

Gene Sequence

>Msa1228290

ATGAATGAAAGGAGAAAAGAAACCCTGCCTGCACTTCACCGCAAATTGAAAGTGATGTTGCTGAACAACCTTAAACGACATTTCTGTTCCAACATCATCACACCAACCTTAAACTCCTCAAAAAAGAAAATCCCTTGTCGCCACAAGAAACTTGCAGTGTCACATGCTCAAAAAGCACTCACTGAATATCTCCATTCCACAAGATCCATCCCTTATGCTTTTGCCGATCAGATTTCCAAAAATTCATTTCATTCTCTCTCTAATCTCATTTCAAAGTTGGGTTCTTTTTCACCAAAAAACTTGGATTCTTTCACCAAAAAAGTTGAAAAGTTCTTGAGGTACAACCCCATCAATGAGTTTGAATTTTTCTTTGAAAGCATTGGAATTCATCATACACGTGTTTCTAGTTTGTTGCCGAACGATAAGTTCTTTTTTTGCGACGATGAATCACTTCTTGATTCTGCTTGTGTGCTTTGTGAATTTGGATTTCCTTGGGATAAGCTTGGTGTGATTTATACAGAGAGTGGTTTTATGTTTAGGAAAAGTGGTTCTGAGATAAAGGGTAGGCTTTGTTTGTTGAAAAGATATGGTTTTTGTAATGTGCAAATTGTTGGGATTTGTTTAACTTTTCCTTTTGTTTTTGGTGCCGAAGAAGGAGGATTTATCGATGATGAGGTTGATAGGTTGCTTTCTGATTTGAGGTTGGTGTTTTTGGATTTTGATTTGGGAGGGTGTGTTGAAGGGAATGTGGATGTTTGGTATGATGTTTGTAGGAAAATTAAAGTGTTTTATGATTTGAGTGATGGTAAGGGGAAGATAGGTGAACTCATTGGAAGGAATAAACATTTGATTCTTGAGTATGGAGAAGAGGAGTTGATTGAAAAGGTTGAGTTTTTTTGTAGATTCAGTGTCGAGAAGGAGGAAATAGCTCGGTTGATTCTACAAGGTTCAGAGTTGTTGAGTCTCAATTTGGAAGCGACGATTAACGTGTTGAAGCTGTTGAAACACATTGGAATGGATTCTAAAGGTCTTGGTGATGCGAAGAAGAACTATGCTCATGCGTTGGGAACAATAAAAATGGCGAATCTTCCCAATCTGATGAGGGCTATGGGTTTACGTGAATGGTTTTTCGATAAAATCAAGGATGGGAATCATAAGCTTTTAGTGGATTTTATCACAAGCTATCCCAATGAAGAGCATGATAAAGGTTATCAAAGTGGTTTTAAGGCAATTCATGATGCAAGGTTACCGTCTCACAATATGAGTAAATTAAATTTCATGCATACTATTGGCTTTGGTGAAAATGCTATAACCATGAATATCTTAAATCACATGCATGGAACAAGTGAAGAGTTACAGAAAAGATTTGATTGCCTTCTACATTTAGGGATTGAATTCTCAAAACTGTGTAAGATAATCACAAAGCTACCAAAGATTCTAAGCCAAAATCCTGAAACTCTAGAGAAAAAGATTAATTTCCTATGTCAGGATATGGGACACTCCTTAGAGCTTTTGGACACTTTTCCGGCATTTATATGTTTTGACTTAGAGAACCGAATTAAGCCGAGGTTCAGATTCCATATGTGGGCTTTAGAAAAGGGATTGTATTCAAAAAACTATTCCATTGCAAGCATGGTTGCGACTAGTGACAAGGGTTTTGTTGGCCGTGTTTTTAAAATTCACCCGGCTGCTCCAAAACATTGGTTTGAGCAATTCTATCCCAATAAATTTCGAGCTTCATGA

Protein sequence

>Msa1228290

MNERRKETLPALHRKLKVMLLNNLKRHFCSNIITPTLNSSKKKIPCRHKKLAVSHAQKALTEYLHSTRSIPYAFADQISKNSFHSLSNLISKLGSFSPKNLDSFTKKVEKFLRYNPINEFEFFFESIGIHHTRVSSLLPNDKFFFCDDESLLDSACVLCEFGFPWDKLGVIYTESGFMFRKSGSEIKGRLCLLKRYGFCNVQIVGICLTFPFVFGAEEGGFIDDEVDRLLSDLRLVFLDFDLGGCVEGNVDVWYDVCRKIKVFYDLSDGKGKIGELIGRNKHLILEYGEEELIEKVEFFCRFSVEKEEIARLILQGSELLSLNLEATINVLKLLKHIGMDSKGLGDAKKNYAHALGTIKMANLPNLMRAMGLREWFFDKIKDGNHKLLVDFITSYPNEEHDKGYQSGFKAIHDARLPSHNMSKLNFMHTIGFGENAITMNILNHMHGTSEELQKRFDCLLHLGIEFSKLCKIITKLPKILSQNPETLEKKINFLCQDMGHSLELLDTFPAFICFDLENRIKPRFRFHMWALEKGLYSKNYSIASMVATSDKGFVGRVFKIHPAAPKHWFEQFYPNKFRAS*