AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene063074

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063074.t1 MTR_3g118360 97.357 946 17 3 4 943 37 980 0.0 1880
MS.gene063074.t1 MTR_3g118360 97.010 836 17 3 4 833 37 870 0.0 1660
MS.gene063074.t1 MTR_3g118360 97.126 835 16 3 4 832 37 869 0.0 1659
MS.gene063074.t1 MTR_3g118360 98.860 702 8 0 242 943 1 702 0.0 1401
MS.gene063074.t1 MTR_4g074640 35.060 830 449 21 46 817 13 810 2.13e-143 451
MS.gene063074.t1 MTR_4g074670 37.792 471 252 13 49 495 16 469 1.31e-92 307
MS.gene063074.t1 MTR_4g074670 36.213 301 171 8 201 495 2 287 5.43e-47 175
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene063074.t1 AT5G22800 74.000 900 231 3 44 941 103 1001 0.0 1384
MS.gene063074.t1 AT5G22800 74.000 900 231 3 44 941 78 976 0.0 1382
MS.gene063074.t1 AT1G50200 36.957 782 408 18 49 774 64 816 5.46e-158 491
MS.gene063074.t1 AT1G50200 36.957 782 408 18 49 774 64 816 7.68e-158 491

Find 217 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGCTTATCTTTGTGAAAA+AGG 0.152053 3.3:+95964344 MS.gene063074:CDS
GTACCTGACACTGAATTTAT+TGG 0.154294 3.3:+95958939 MS.gene063074:CDS
ACACTAATCCATAATCTTTC+TGG 0.181990 3.3:-95957396 None:intergenic
CAATTCAAACCTATCTTTCT+TGG 0.208066 3.3:+95957177 MS.gene063074:CDS
AATACTTTCTGAGCAATTAT+AGG 0.215096 3.3:-95958540 None:intergenic
ATTCAGGCAGGTAAATTTAT+TGG 0.228211 3.3:+95964206 MS.gene063074:CDS
TACTCCAGGGGTGGTTGGTC+AGG 0.230750 3.3:+95964181 MS.gene063074:CDS
GTTTGCCTCCAGAAAGATTA+TGG 0.233077 3.3:+95957388 MS.gene063074:CDS
AAGTCAGTTTCATAGTTATT+TGG 0.248121 3.3:-95958161 None:intergenic
AAGTGCCGCAGAATATTTAA+TGG 0.255077 3.3:+95964082 MS.gene063074:CDS
ATCCTCACAGTTACTTCTTC+AGG 0.258382 3.3:-95962086 None:intergenic
GACCTCTCAAGCTGGTTCTT+TGG 0.278405 3.3:+95959521 MS.gene063074:CDS
ATAGATGTTCAGAAGTCTTT+TGG 0.278583 3.3:+95959164 MS.gene063074:CDS
GGCAGGTTGCTTGGTATAAA+AGG 0.291942 3.3:+95958483 MS.gene063074:CDS
TCTATGTTCTTCTGCTTTAG+AGG 0.298105 3.3:-95957853 None:intergenic
TGGGAGGAAGCCTGAAAATT+TGG 0.299980 3.3:+95964289 MS.gene063074:CDS
CCTCCTGATTCAGCATAAAA+TGG 0.304165 3.3:-95959071 None:intergenic
GGTGGTTGGTCAGGGAATTC+AGG 0.315604 3.3:+95964190 MS.gene063074:CDS
CTACCCGTAACTCCTCTAAA+AGG 0.316525 3.3:-95962115 None:intergenic
GACTTATCTACCATTGAGTT+TGG 0.319082 3.3:+95957366 MS.gene063074:CDS
ACTGTTCTCCTCACCATTGC+TGG 0.323081 3.3:+95957147 MS.gene063074:CDS
GAAGGAGCAATTGCAATGTT+TGG 0.329419 3.3:+95959693 MS.gene063074:CDS
AAGCAGAAGAACATAGATAC+TGG 0.331732 3.3:+95957859 MS.gene063074:CDS
CAACCTCTAGAACACCCTTT+TGG 0.351759 3.3:-95959214 None:intergenic
TAACCCCTCCCAACGTTTGA+TGG 0.352810 3.3:-95958423 None:intergenic
AACCTCTTAATTCGGTCAAC+GGG 0.355293 3.3:-95957557 None:intergenic
GTGATCCAGCCGTACAGGTT+AGG 0.360098 3.3:+95959012 MS.gene063074:CDS
CAGGATACTAGTTGAGTGTT+TGG 0.364396 3.3:+95964034 MS.gene063074:intron
AGGAGAACAGTGGGGTCATC+TGG 0.366681 3.3:-95957135 None:intergenic
ACTACAAGCAGCGCTTAAAA+AGG 0.368159 3.3:+95959485 MS.gene063074:CDS
AACAAGCTATTCAATGGGCT+TGG 0.372665 3.3:+95957343 MS.gene063074:CDS
TTACCTGTTGAGACCATAAC+TGG 0.378573 3.3:-95957443 None:intergenic
GTAGCAAGCCTAGTGGTAAA+TGG 0.391692 3.3:+95958990 MS.gene063074:CDS
CTTTCAGATACCTCTTTGTC+TGG 0.392755 3.3:-95958247 None:intergenic
GAATTAGCAAATATTTCATA+TGG 0.402355 3.3:+95958212 MS.gene063074:CDS
GTCAAGCTTGCCATTGGAAG+CGG 0.403782 3.3:+95958897 MS.gene063074:CDS
CATAGATACTGGGCTTGGTC+TGG 0.405421 3.3:+95957870 MS.gene063074:CDS
ATCATGAGACTTTCCAGTTA+TGG 0.406423 3.3:+95957430 MS.gene063074:CDS
CCCACCAGCCTGAGCGAAGT+TGG 0.409388 3.3:-95964270 None:intergenic
TGGTGGCATTTGATCGTCTC+AGG 0.410015 3.3:+95959541 MS.gene063074:CDS
CTTCACCCAACCTCTTAATT+CGG 0.423735 3.3:-95957565 None:intergenic
GTACGTGTTGTAGAGGTTCC+TGG 0.424531 3.3:+95960844 MS.gene063074:CDS
ATTATCTCCACGACAGCCTT+AGG 0.424823 3.3:-95959143 None:intergenic
TTTCCAGTTATGGTCTCAAC+AGG 0.427549 3.3:+95957440 MS.gene063074:CDS
ACTCCAGGGGTGGTTGGTCA+GGG 0.433011 3.3:+95964182 MS.gene063074:CDS
CATACCTTCTTTGAGATGCT+TGG 0.433272 3.3:+95957291 MS.gene063074:CDS
TTATGTGGTGGTGGAGGAGG+TGG 0.436304 3.3:+95964242 MS.gene063074:CDS
GGGTCTTGAAAGCAGGATCT+TGG 0.437253 3.3:+95957754 MS.gene063074:intron
TGGGTGAAGAGGATAACTTT+TGG 0.438035 3.3:+95957579 MS.gene063074:CDS
TCAGAGCAGGGTATTGCATC+TGG 0.441838 3.3:+95960937 MS.gene063074:CDS
CTCTCAAGCTGGTTCTTTGG+TGG 0.451414 3.3:+95959524 MS.gene063074:CDS
TTCAGAGGCCTTCTCTATGA+TGG 0.452809 3.3:-95958192 None:intergenic
AGGCGAATAGAAGCTGTAGC+TGG 0.454459 3.3:+95960964 MS.gene063074:CDS
ATGCTTGGTAACTTCAGCTT+TGG 0.456959 3.3:+95957306 MS.gene063074:CDS
ATTGTCCTGGGCTCATGTCC+AGG 0.459686 3.3:+95964128 MS.gene063074:CDS
TATCCAATAAATTCAGTGTC+AGG 0.460034 3.3:-95958942 None:intergenic
AGGGTATTGCATCTGGTGTC+AGG 0.461565 3.3:+95960944 MS.gene063074:CDS
CTTCAACTCCTCAAGGATAC+GGG 0.462475 3.3:-95958607 None:intergenic
TGATCCAGCCGTACAGGTTA+GGG 0.465370 3.3:+95959013 MS.gene063074:CDS
AGTAATACTTCTGAAATTCG+TGG 0.467614 3.3:+95960901 MS.gene063074:CDS
TCACAAGGGTACAGTCCAAA+AGG 0.467810 3.3:+95959199 MS.gene063074:CDS
GGAACTGTACCTTGCTTGGC+TGG 0.471150 3.3:+95958723 MS.gene063074:CDS
ATTCCCTGACCAACCACCCC+TGG 0.472687 3.3:-95964185 None:intergenic
GGAGGACAAATTGCAGATCA+TGG 0.477163 3.3:+95959089 MS.gene063074:CDS
CATGGCTCGCATTCTTCAAA+AGG 0.478575 3.3:+95957897 MS.gene063074:CDS
ACAAGCTATTCAATGGGCTT+GGG 0.479029 3.3:+95957344 MS.gene063074:CDS
GGTAGCAAGAAACGAAAATT+CGG 0.480512 3.3:+95962133 MS.gene063074:CDS
TCCCCGTTGACCGAATTAAG+AGG 0.480923 3.3:+95957555 MS.gene063074:CDS
TCTTCAACTCCTCAAGGATA+CGG 0.483308 3.3:-95958608 None:intergenic
TCCAACTTCGCTCAGGCTGG+TGG 0.485257 3.3:+95964269 MS.gene063074:CDS
GCATCGGTGTATCCCCTCTC+AGG 0.486058 3.3:-95957659 None:intergenic
AAATTATGTGGTGGTGGAGG+AGG 0.487864 3.3:+95964239 MS.gene063074:CDS
TTTCTGCAAGCTCGCTATCA+AGG 0.488467 3.3:-95959590 None:intergenic
AGAGCAAGGACCGCAGGGAC+CGG 0.491524 3.3:-95957615 None:intergenic
ATTAAGAAATCTTTCCCGTA+TGG 0.492373 3.3:-95957064 None:intergenic
GTTGACCGAATTAAGAGGTT+GGG 0.494052 3.3:+95957560 MS.gene063074:CDS
GTTCGTACAGGCAGGTTGCT+TGG 0.494701 3.3:+95958474 MS.gene063074:CDS
TTGGTATAAAAGGTGATGGT+AGG 0.500325 3.3:+95958493 MS.gene063074:CDS
AAATGGTGATCCAGCCGTAC+AGG 0.500392 3.3:+95959007 MS.gene063074:CDS
ATCAGAAGAGTTGTTCGTAC+AGG 0.503028 3.3:+95958462 MS.gene063074:CDS
GGCGAATAGAAGCTGTAGCT+GGG 0.506427 3.3:+95960965 MS.gene063074:CDS
GAAGAGTTGTTCGTACAGGC+AGG 0.512237 3.3:+95958466 MS.gene063074:CDS
CTGGATCACCATTTACCACT+AGG 0.515077 3.3:-95958998 None:intergenic
TGATACTTATGGTTTCCCTA+TGG 0.515111 3.3:+95958767 MS.gene063074:CDS
CATTGTTGATATCATTAGTA+CGG 0.517063 3.3:-95957248 None:intergenic
TCAGAGGCCTTCTCTATGAT+GGG 0.518722 3.3:-95958191 None:intergenic
TGGTATAAAAGGTGATGGTA+GGG 0.518928 3.3:+95958494 MS.gene063074:CDS
GCGTTGTACCATCAAACGTT+GGG 0.520683 3.3:+95958415 MS.gene063074:CDS
GCAAAATTATGTGGTGGTGG+AGG 0.520739 3.3:+95964236 MS.gene063074:CDS
GTACCATCAAACGTTGGGAG+GGG 0.521676 3.3:+95958420 MS.gene063074:CDS
GTTACCAAGCATCTCAAAGA+AGG 0.522266 3.3:-95957295 None:intergenic
CAAAAGGGTGTTCTAGAGGT+TGG 0.522615 3.3:+95959215 MS.gene063074:CDS
CTCTGATTTAGCTTTCTCGA+GGG 0.522771 3.3:-95964318 None:intergenic
AATTTCAGAGCAAGGACCGC+AGG 0.525249 3.3:-95957621 None:intergenic
ATTGTGTTTCTCATCTCTGA+TGG 0.528181 3.3:+95958393 MS.gene063074:CDS
CCTTCCCTAACCTGTACGGC+TGG 0.530381 3.3:-95959017 None:intergenic
AGGTCCAACTTCGCTCAGGC+TGG 0.530546 3.3:+95964266 MS.gene063074:CDS
CTTCACCTGGACATGAGCCC+AGG 0.531541 3.3:-95964133 None:intergenic
TGGGCTTGGTCTGGAGCGCA+TGG 0.533587 3.3:+95957879 MS.gene063074:CDS
GCTTCGATTTGTGCAGACGC+TGG 0.536077 3.3:+95958638 MS.gene063074:CDS
TGGAAGGTCCAACTTCGCTC+AGG 0.537180 3.3:+95964262 MS.gene063074:CDS
AATACTGTCAAGCTTGCCAT+TGG 0.538095 3.3:+95958891 MS.gene063074:CDS
GAGCCCAGGACAATAGCTGC+TGG 0.539000 3.3:-95964119 None:intergenic
GAATTGAAGCATTCCAGCAA+TGG 0.539001 3.3:-95957160 None:intergenic
GGAGGGGTTATGTAGTTCGA+CGG 0.540107 3.3:+95958436 MS.gene063074:CDS
CTGGGCTCATGTCCAGGTGA+AGG 0.540402 3.3:+95964134 MS.gene063074:CDS
AAGAACATAGATACTGGGCT+TGG 0.541349 3.3:+95957865 MS.gene063074:CDS
AGACTAACCTTCCCTTCACC+TGG 0.541694 3.3:-95964146 None:intergenic
ACATTAGCAGTAGCAGCAGC+AGG 0.541792 3.3:+95956931 MS.gene063074:CDS
GGAAAGTCTCATGATCGTCT+TGG 0.542083 3.3:-95957422 None:intergenic
TTTAAAGTAATTTCAGAGCA+GGG 0.545061 3.3:+95960925 MS.gene063074:CDS
AGATCCAGCAGCTATTGTCC+TGG 0.546412 3.3:+95964115 MS.gene063074:CDS
TCAAGCTTGCCATTGGAAGC+GGG 0.546840 3.3:+95958898 MS.gene063074:CDS
GGTCAGGAGACCTCTCAAGC+TGG 0.547419 3.3:+95959513 MS.gene063074:CDS
CGTTGACCGAATTAAGAGGT+TGG 0.549152 3.3:+95957559 MS.gene063074:CDS
GATCCAGCAGCTATTGTCCT+GGG 0.550834 3.3:+95964116 MS.gene063074:CDS
GTTGGTCAGGGAATTCAGGC+AGG 0.558788 3.3:+95964194 MS.gene063074:CDS
CACAAGGGTACAGTCCAAAA+GGG 0.558794 3.3:+95959200 MS.gene063074:CDS
ATATCAAGTGCTGAAAGAAG+TGG 0.561924 3.3:+95958702 MS.gene063074:CDS
GTAGCACGAGGGACTTGTCT+AGG 0.562012 3.3:-95957207 None:intergenic
TGGAGGCAAACCAAACTCAA+TGG 0.563536 3.3:-95957376 None:intergenic
CCAACTTCGCTCAGGCTGGT+GGG 0.563594 3.3:+95964270 MS.gene063074:CDS
GATAATAATAATTGTGTGAG+TGG 0.564463 3.3:+95957036 MS.gene063074:CDS
ACTCCCTTCCCTAACCTGTA+CGG 0.564727 3.3:-95959021 None:intergenic
TTATGCTTCCCGTGGTCACA+AGG 0.565846 3.3:+95957089 MS.gene063074:CDS
TGGCAATATATTTGTTCACA+AGG 0.567482 3.3:+95959184 MS.gene063074:CDS
ATCAGCAAGAGGCATCACTT+TGG 0.568344 3.3:-95959661 None:intergenic
AATTAAGAGGTTGGGTGAAG+AGG 0.568929 3.3:+95957568 MS.gene063074:CDS
ATAGCAAAATTATGTGGTGG+TGG 0.571171 3.3:+95964233 MS.gene063074:CDS
TCATAATAAATTTCAGAGCA+AGG 0.571821 3.3:-95957629 None:intergenic
AAAGTTGAGGCAACGAGCCA+AGG 0.572093 3.3:+95959265 MS.gene063074:CDS
CAACCTCTTAATTCGGTCAA+CGG 0.573322 3.3:-95957558 None:intergenic
TGTACCATCAAACGTTGGGA+GGG 0.573784 3.3:+95958419 MS.gene063074:CDS
CACCAAAGAACCAGCTTGAG+AGG 0.573822 3.3:-95959523 None:intergenic
CTTCTCTCTTCAACTCCTCA+AGG 0.574968 3.3:-95958614 None:intergenic
GTGTGAGTGGAGATTCCATA+CGG 0.575858 3.3:+95957049 MS.gene063074:CDS
TTGCGACGATGATGTACAGA+AGG 0.575956 3.3:-95957007 None:intergenic
TGAAATATTTGCTAATTCAG+AGG 0.579198 3.3:-95958207 None:intergenic
ACTAATGATATCAACAATGT+TGG 0.579654 3.3:+95957252 MS.gene063074:CDS
TATGGAAATAACAAAAGAAG+TGG 0.579964 3.3:+95958785 MS.gene063074:CDS
CTCATGTCCAGGTGAAGGGA+AGG 0.580776 3.3:+95964139 MS.gene063074:CDS
GTGGTGGTGGAGGAGGTGGA+AGG 0.581825 3.3:+95964246 MS.gene063074:CDS
TGGGCTCATGTCCAGGTGAA+GGG 0.582195 3.3:+95964135 MS.gene063074:CDS
AGCAGAAGAACATAGATACT+GGG 0.583073 3.3:+95957860 MS.gene063074:CDS
AGGAAGGACCTTGTGACCAC+GGG 0.584439 3.3:-95957097 None:intergenic
GCAATGGTGAGGAGAACAGT+GGG 0.584917 3.3:-95957144 None:intergenic
AAGTGGAACTGTACCTTGCT+TGG 0.584929 3.3:+95958719 MS.gene063074:CDS
AATACTTGTCCCGCTTCCAA+TGG 0.585244 3.3:-95958907 None:intergenic
TTGTACCATCAAACGTTGGG+AGG 0.589872 3.3:+95958418 MS.gene063074:CDS
GAGAGGAGAGAAATTGCTTG+AGG 0.590060 3.3:+95958662 MS.gene063074:CDS
GGTATAAAAGGTGATGGTAG+GGG 0.590969 3.3:+95958495 MS.gene063074:CDS
GGCAATATATTTGTTCACAA+GGG 0.592455 3.3:+95959185 MS.gene063074:CDS
TGGGGCATTCAAAACAGTAC+AGG 0.592610 3.3:+95962216 MS.gene063074:CDS
CAAACAAGTGGAAGCAACAG+TGG 0.594498 3.3:+95959238 MS.gene063074:CDS
GCTGATCAACGGATGGATTG+AGG 0.596209 3.3:+95959620 MS.gene063074:CDS
AGAGGTTCCTGGAGTATCGA+TGG 0.596716 3.3:+95960855 MS.gene063074:CDS
TTGCTTGGTATAAAAGGTGA+TGG 0.598004 3.3:+95958489 MS.gene063074:CDS
AGTCCAAAAGGGTGTTCTAG+AGG 0.604342 3.3:+95959211 MS.gene063074:CDS
AGGAAGCCTGAAAATTTGGC+TGG 0.604884 3.3:+95964293 MS.gene063074:CDS
TTTGTGCAGACGCTGGAGAG+AGG 0.605643 3.3:+95958645 MS.gene063074:CDS
TGTTTCCATTAAATATTCTG+CGG 0.605968 3.3:-95964087 None:intergenic
CAAAGTTCCATCGATACTCC+AGG 0.606263 3.3:-95960862 None:intergenic
CGCTATCAAGGAGTGGACGG+TGG 0.606601 3.3:-95959578 None:intergenic
AGGATCTTGGTGATGATACA+CGG 0.607079 3.3:+95957767 MS.gene063074:intron
GATTCTCCCCATCATAGAGA+AGG 0.608580 3.3:+95958184 MS.gene063074:CDS
GGACAGATAGCAAAATTATG+TGG 0.609892 3.3:+95964227 MS.gene063074:CDS
GCTCTGATTTAGCTTTCTCG+AGG 0.610643 3.3:-95964319 None:intergenic
GAAATCGAACTGCTGATCAA+CGG 0.611385 3.3:+95959609 MS.gene063074:CDS
CCAGCCGTACAGGTTAGGGA+AGG 0.611758 3.3:+95959017 MS.gene063074:CDS
GAACGAGAGAAGCACTAGGA+AGG 0.612505 3.3:-95957113 None:intergenic
TCAGGAACATACTTTGAGAG+TGG 0.613102 3.3:-95961043 None:intergenic
TATCATACTACTTACAGCAT+CGG 0.614418 3.3:-95957675 None:intergenic
TCGAACTGCTGATCAACGGA+TGG 0.614833 3.3:+95959613 MS.gene063074:CDS
GAATCAACCTAAGGCTGTCG+TGG 0.615063 3.3:+95959136 MS.gene063074:CDS
GGCGTTGTACCATCAAACGT+TGG 0.615289 3.3:+95958414 MS.gene063074:CDS
ATTTCAGAGCAAGGACCGCA+GGG 0.616018 3.3:-95957620 None:intergenic
AGAGGGTGAGAATCAACCTA+AGG 0.616634 3.3:+95959127 MS.gene063074:CDS
TAGGAAGGACCTTGTGACCA+CGG 0.617961 3.3:-95957098 None:intergenic
ACTTCGCTCAGGCTGGTGGG+AGG 0.619056 3.3:+95964273 MS.gene063074:CDS
GGAGTATCGATGGAACTTTG+TGG 0.622057 3.3:+95960865 MS.gene063074:CDS
CTAATCCATAATCTTTCTGG+AGG 0.622240 3.3:-95957393 None:intergenic
GCTCGCTATCAAGGAGTGGA+CGG 0.625144 3.3:-95959581 None:intergenic
TGAAGGGAAGGTTAGTCTAG+TGG 0.626264 3.3:+95964151 MS.gene063074:CDS
TCTAGAGGTTGGCAAACAAG+TGG 0.626675 3.3:+95959226 MS.gene063074:CDS
AGCAATGGTGAGGAGAACAG+TGG 0.627721 3.3:-95957145 None:intergenic
GGTTAGGGAAGGGAGTAATG+TGG 0.629772 3.3:+95959028 MS.gene063074:CDS
GTGTTTGGATGATGTTGATG+CGG 0.629997 3.3:+95964049 MS.gene063074:CDS
GGTGTGAGTATTGACATGAA+TGG 0.634865 3.3:+95958819 MS.gene063074:CDS
GAAGCATTCCAGCAATGGTG+AGG 0.639478 3.3:-95957155 None:intergenic
GCAAGCTCGCTATCAAGGAG+TGG 0.639731 3.3:-95959585 None:intergenic
CGTTGTGAAGTAGTAGCACG+AGG 0.644204 3.3:-95957219 None:intergenic
GATGGTAGGGGAGACCCTGA+AGG 0.644605 3.3:+95958507 MS.gene063074:CDS
ACTGCAGGTACGTGTTGTAG+AGG 0.644859 3.3:+95960837 MS.gene063074:intron
TATCGATGGAACTTTGTGGT+GGG 0.645529 3.3:+95960869 MS.gene063074:CDS
CAGCCGTACAGGTTAGGGAA+GGG 0.646194 3.3:+95959018 MS.gene063074:CDS
AGCGGAGTCACCGGTCCCTG+CGG 0.646446 3.3:+95957605 MS.gene063074:CDS
TAGAGCACCCCGTATCCTTG+AGG 0.648075 3.3:+95958599 MS.gene063074:CDS
TGCTTGTAGTAAATGAGTAG+CGG 0.651476 3.3:-95959472 None:intergenic
CAACAGTGGATGTAAAGTTG+AGG 0.654944 3.3:+95959252 MS.gene063074:CDS
TGTGAGTGGAGATTCCATAC+GGG 0.656667 3.3:+95957050 MS.gene063074:CDS
CAGAGGCCTTCTCTATGATG+GGG 0.657776 3.3:-95958190 None:intergenic
GCGAATAGAAGCTGTAGCTG+GGG 0.661208 3.3:+95960966 MS.gene063074:CDS
TATCTGATGACCAGACAAAG+AGG 0.661772 3.3:+95958237 MS.gene063074:CDS
TTCAACTCCTCAAGGATACG+GGG 0.663705 3.3:-95958606 None:intergenic
AAAGAAGGTATGATGACGTG+AGG 0.668436 3.3:-95957280 None:intergenic
ACCTCTTAATTCGGTCAACG+GGG 0.679351 3.3:-95957556 None:intergenic
ACATCTTCACCAGCCAAGCA+AGG 0.684496 3.3:-95958732 None:intergenic
AGCAATTGTAGCAAGCCTAG+TGG 0.687149 3.3:+95958983 MS.gene063074:CDS
TTAGCAGTAGCAGCAGCAGG+AGG 0.688487 3.3:+95956934 MS.gene063074:CDS
AAAGAAGTGGCTGAAGAACG+CGG 0.692053 3.3:+95958798 MS.gene063074:CDS
TGCGACGATGATGTACAGAA+GGG 0.698896 3.3:-95957006 None:intergenic
GTATCGATGGAACTTTGTGG+TGG 0.704292 3.3:+95960868 MS.gene063074:CDS
TCTGGAACGAGAGAAGCACT+AGG 0.708872 3.3:-95957117 None:intergenic
AACCTGAAGAAGTAACTGTG+AGG 0.719116 3.3:+95962084 MS.gene063074:CDS
CAGATAGCAAAATTATGTGG+TGG 0.721808 3.3:+95964230 MS.gene063074:CDS
GTTGTGAAGTAGTAGCACGA+GGG 0.763537 3.3:-95957218 None:intergenic
CAATGGTGAGGAGAACAGTG+GGG 0.772634 3.3:-95957143 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr3.3 gene 95956922 95964373 95956922 ID=MS.gene063074
chr3.3 mRNA 95956922 95964373 95956922 ID=MS.gene063074.t1;Parent=MS.gene063074
chr3.3 exon 95956922 95957461 95956922 ID=MS.gene063074.t1.exon1;Parent=MS.gene063074.t1
chr3.3 CDS 95956922 95957461 95956922 ID=cds.MS.gene063074.t1;Parent=MS.gene063074.t1
chr3.3 exon 95957548 95957682 95957548 ID=MS.gene063074.t1.exon2;Parent=MS.gene063074.t1
chr3.3 CDS 95957548 95957682 95957548 ID=cds.MS.gene063074.t1;Parent=MS.gene063074.t1
chr3.3 exon 95957769 95957918 95957769 ID=MS.gene063074.t1.exon3;Parent=MS.gene063074.t1
chr3.3 CDS 95957769 95957918 95957769 ID=cds.MS.gene063074.t1;Parent=MS.gene063074.t1
chr3.3 exon 95958158 95958268 95958158 ID=MS.gene063074.t1.exon4;Parent=MS.gene063074.t1
chr3.3 CDS 95958158 95958268 95958158 ID=cds.MS.gene063074.t1;Parent=MS.gene063074.t1
chr3.3 exon 95958369 95959286 95958369 ID=MS.gene063074.t1.exon5;Parent=MS.gene063074.t1
chr3.3 CDS 95958369 95959286 95958369 ID=cds.MS.gene063074.t1;Parent=MS.gene063074.t1
chr3.3 exon 95959462 95959734 95959462 ID=MS.gene063074.t1.exon6;Parent=MS.gene063074.t1
chr3.3 CDS 95959462 95959734 95959462 ID=cds.MS.gene063074.t1;Parent=MS.gene063074.t1
chr3.3 exon 95960844 95961053 95960844 ID=MS.gene063074.t1.exon7;Parent=MS.gene063074.t1
chr3.3 CDS 95960844 95961053 95960844 ID=cds.MS.gene063074.t1;Parent=MS.gene063074.t1
chr3.3 exon 95962080 95962237 95962080 ID=MS.gene063074.t1.exon8;Parent=MS.gene063074.t1
chr3.3 CDS 95962080 95962237 95962080 ID=cds.MS.gene063074.t1;Parent=MS.gene063074.t1
chr3.3 exon 95964037 95964373 95964037 ID=MS.gene063074.t1.exon9;Parent=MS.gene063074.t1
chr3.3 CDS 95964037 95964373 95964037 ID=cds.MS.gene063074.t1;Parent=MS.gene063074.t1
Gene Sequence

>MS.gene063074

ATGAATGTTACATTAGCAGTAGCAGCAGCAGGAGGATTTATCTCTTTCAAACAACTACATCTACGTCGTAGTCGTCGTCTTCTTCCCTTCTGTACATCATCGTCGCAAGACAATGATAATAATAATTGTGTGAGTGGAGATTCCATACGGGAAAGATTTCTTAATTTTTATGCTTCCCGTGGTCACAAGGTCCTTCCTAGTGCTTCTCTCGTTCCAGATGACCCCACTGTTCTCCTCACCATTGCTGGAATGCTTCAATTCAAACCTATCTTTCTTGGAAAACTTCCTAGACAAGTCCCTCGTGCTACTACTTCACAACGCTGCATCCGTACTAATGATATCAACAATGTTGGTCATACCTCACGTCATCATACCTTCTTTGAGATGCTTGGTAACTTCAGCTTTGGTGATTACTTCAAAAAACAAGCTATTCAATGGGCTTGGGACTTATCTACCATTGAGTTTGGTTTGCCTCCAGAAAGATTATGGATTAGTGTTTACCAAGACGATCATGAGACTTTCCAGTTATGGTCTCAACAGGTAATTACATCACAATCACATTTCCTTTTAATTACCCCTCTCCTCTTCCGTTCCTGTCGTCACATCACATTTTCATTTCTCTGCAGGTTGGTGTCCCCGTTGACCGAATTAAGAGGTTGGGTGAAGAGGATAACTTTTGGACAAGCGGAGTCACCGGTCCCTGCGGTCCTTGCTCTGAAATTTATTATGATTTTTATCCTGAGAGGGGATACACCGATGCTGTAAGTAGTATGATATACTTGTTACAAATTGAGTTATTTCAAATCACTTCACTGACAATGTTTTTTGGCTTGGGTCTTGAAAGCAGGATCTTGGTGATGATACACGGTTTATAGAGTTCTACAATTTAGTATTCATGCAATACAACAAAAAAGACGATGGTTCTCTCGAACCTCTAAAGCAGAAGAACATAGATACTGGGCTTGGTCTGGAGCGCATGGCTCGCATTCTTCAAAAGGTATTTCATTCTTTTGAATTGTTGTAAAAGTGAGTGAGTATTTGTACTTTTACAAGTGTTTCTAGGAGACTAGAACTGGAACTATGATATTTCTGCACCCTACACCACATCGTCGCTTAATGTTGAGAAAAGTGGGGCAAGGAAAAAGGGTGTGAATGAAACATTACTCACAAATCACAATACTCTCATTTTCATTTGCTCCTCAAGTCCTTTTTATATTTATTCATTATTCATTGCAGGCTCCAAATAACTATGAAACTGACTTGATTCTCCCCATCATAGAGAAGGCCTCTGAATTAGCAAATATTTCATATGGCATATCTGATGACCAGACAAAGAGGTATCTGAAAGTAAGTGTGACTATCTTCATACAAATTGTGGTAGAATTTTAAATAGTTTATTTCAATTCGCCTTATCACTTTTAATTTATGCTATTTTTCCTTTACTTAGATTGTAGGAGATCACATGCGTGCAATTGTGTTTCTCATCTCTGATGGCGTTGTACCATCAAACGTTGGGAGGGGTTATGTAGTTCGACGGCTTATCAGAAGAGTTGTTCGTACAGGCAGGTTGCTTGGTATAAAAGGTGATGGTAGGGGAGACCCTGAAGGAGCATTTTTACCTATAATTGCTCAGAAAGTATTAGAGTTAAGCACTCACATTGATGCTGATGTGAAGAATAGAGCACCCCGTATCCTTGAGGAGTTGAAGAGAGAAGAGCTTCGATTTGTGCAGACGCTGGAGAGAGGAGAGAAATTGCTTGAGGAGAAGCTTGTTGATGCTATATCAAGTGCTGAAAGAAGTGGAACTGTACCTTGCTTGGCTGGTGAAGATGTGTTTATTTTGTATGATACTTATGGTTTCCCTATGGAAATAACAAAAGAAGTGGCTGAAGAACGCGGTGTGAGTATTGACATGAATGGTTTTGATATTGAGATGGAGAAGCAAAGACGTCAATCTCAAGCTGCACACAATACTGTCAAGCTTGCCATTGGAAGCGGGACAAGTATTGCAGACAATGTACCTGACACTGAATTTATTGGATATGAAAGTCTTTATTGTAAAGCAATTGTAGCAAGCCTAGTGGTAAATGGTGATCCAGCCGTACAGGTTAGGGAAGGGAGTAATGTGGAAATTTTGCTGAACAAGACTCCATTTTATGCTGAATCAGGAGGACAAATTGCAGATCATGGTTTTCTGTATATATCAGAGGGTGAGAATCAACCTAAGGCTGTCGTGGAGATAATAGATGTTCAGAAGTCTTTTGGCAATATATTTGTTCACAAGGGTACAGTCCAAAAGGGTGTTCTAGAGGTTGGCAAACAAGTGGAAGCAACAGTGGATGTAAAGTTGAGGCAACGAGCCAAGGTACAAAGACTGTGTACCCACTCATTTTATTTATTTACTTCTTTGGTGAATCTTAAGTCATAAATAACAGAATTTGATACGGACAAAAATTTACATCATCATAGTTATGCCTATTTCCAAATCCGAATCTTACAAGTTGTTCAAGATTACAGTAAATATAATTGGTGCTTTGCAGGTTCATCATACCGCTACTCATTTACTACAAGCAGCGCTTAAAAAGGTCATTGGTCAGGAGACCTCTCAAGCTGGTTCTTTGGTGGCATTTGATCGTCTCAGGTTTGATTTCAATTTCCACCGTCCACTCCTTGATAGCGAGCTTGCAGAAATCGAACTGCTGATCAACGGATGGATTGAGGATGCGATTCTTCTTCAGACCAAAGTGATGCCTCTTGCTGATGCAAAAAGAGAAGGAGCAATTGCAATGTTTGGAGAAAAATACGGGGAAGAGGTTGGACTTGCTACAAACGCTTTTGATATTTAACTTTGAAATTAATTTAGCTTCTGTTTTGGTAGTTTTAAACATGAAGTGTGTACATAAAAGAGCTCAGAAATTTGACCTTCAGGGATGGATTGCAACTAATGTTCAGGAATCATCATGTCTTCTGACCTAAATTCCCACATTACTATTACTATTGCCAGATTCCAATTACATTGTGATTTCAGATGCATCAATAGTTATCCTTCTCTCTCTCTATCACTTGTACCAGCACCTACACATGGACCATAGTAGTGAAATATACGGCATAGTAATATACTAGATTCTGAAAAAAAATTAAGAGCATCCAGCTGTGAATTCTTAAACTGTACAAACGAAATGTAACCCCTAACAGCCCACAACCATGTCTACATCTAAGGTTGGTTATGACTTTGATTGGCAAAATTTTATTATTAGTGGCAGCTAGTGCCTAGTTGTTAGCAGCTTGGCTTATAGCTAAAAGTGGCCATGCTAACTGTCTGATGTTAAAGTATCTGATAAAGTTAGTGGTAAACTCGCTGATAAATATAAAATGACATGTCTATCTTTTTTTAGATAAATTGTGTTTATGTGTAGTATTGATTTGTAAATTATTATCGCTTTTAATACTTAAATTTATTTAATATACTTCTCATTTCTACATAAAATGCGATAATGCTAACAAAATGACATGTACACATAATTCTTTCATATAAATTTTAAGATTTTACAGGAAAATATATACTCTCCAGGGGCTAGGATTCTTTTGTTTTAAAGGGAGTATATAATTAATATATTGAGAGTAAAAAGAAAAAAGAAAATAATACAATACAGACTATAGGCTCAGATGCTATATGAAATATAACACAAAAATAACCCTATAAAGTAGTGGGATAAGTTTCTTTATCAAACAACTCTAGTTATTAAATCCACTATAAACCAGTCAGGTCCACCGTAAGCTACTGGATCAAAGTTGCCAATGAGATTATATATGTTCCATATTTTAGACATTTATATTTCTACTTCTCAATAATCGGCGAAAATAACCTGTTACTTCTCAATAACCTGTTTCTTCTTGTGTAATCATATTTGGTGTACTGCAGGTACGTGTTGTAGAGGTTCCTGGAGTATCGATGGAACTTTGTGGTGGGACTCATGTCAGTAATACTTCTGAAATTCGTGGTTTTAAAGTAATTTCAGAGCAGGGTATTGCATCTGGTGTCAGGCGAATAGAAGCTGTAGCTGGGGAAGCTTTCATCGAATATGTCAATGCTCGAGATTTATATCTGAAACAGCTATGTTCCACTCTCAAAGTATGTTCCTGATTTCAGTGTTTCAGAATTTTGTGCAAATAGTATAAATTTGTACAGAGTAGTTAATATCCATGACCACGAGTCATGCAATTGATATGACCAATACTTAATAATATAAATTTGTGCGCATAGGTATCTATTTGTTTTAAGGGGGACCCTTGGCACAACGGTAAAGTTGTTGTCATGTGACTAAAAGGTCACCGGTTCAAGTCCTATAAACAGCCTCGTGTAAAAAACAGGTTAAGGCTGCGTATAATACACCAAAATGGCGGGACCCCTTCTCGGACCCTGCGTATGTGGGAGCTTTTTTGCACCGGGCTGCCTTTTATAGGTATCTGTTTGTTCTCCTTCCCCTTTGTTGGGGTCGAGTACACAGAAGAATTAGGGTTGAGAGACAAACCATTATATTTTTTACAAGATGGGATTCAATCACAATATGGGTAGGGTTTTCCATGTGGGTGTGGCTCAGTTGGTTATGTAGAATTGAGTTTATGGGGGAGGACCAAGATTCGATCCCCGCAGAACACACTATTGGGGAGGGGTGAAGAGCTTTCAGCTGCAACCTAGGCACTCGAGCGGAGATTAGTCCCATTGCTTGTCCAAAGGACTGAAGTCTGCTGTGATACTCTGTGGAGCCACTGAGGTGTCAAAGGCCCTAATTACAGCGTTTTATTACCAAAGCCAAAATATTTACTACGACATTGTCATGCCTTACAAAGATTTTGTAGTTTGTTTCCTTGTGTATTATACATATAAGATAAGGGAACCGTCTCACTTTTTTCATGCTCGCACATTTGGGGTTCCTTGTTGCATGCGTTGCTCAATACATAGAGTAGTATTTCCTTTATGTCTACAATAATCTTTAACATGAATTTTTAATAAAAAAGGGCAGCCCAGTGCACTAAAGCTCCCACGTACGCAGGGTCCGGGGAAAGGGTCCTACCATTTTGGTGTATTGTAGATTTTGACGTTTTCTGCATCTGGTACCATTTCTATCCTTGAATAACTTATAATCAACTTTTGAAGGTTAAACCTGAAGAAGTAACTGTGAGGATAGAAAACCTTTTAGAGGAGTTACGGGTAGCAAGAAACGAAAATTCGGCTGTGCGTGCAAAAGCAGCAGTCTACAAAGCTTCAGTTATTGCAAGCAGAGCGTTTTTGGTGGGGCATTCAAAACAGTACAGGTAATACTTCTCCATCTTGTAGTATTTGTTACTTTAATATGGGTTTGGGAGAGAATCTTCTCCCTTGTTAGCAACACCAAAAAAAGTTTTTCTGATTAGATAATTTATGCCTGTAAGGTACAAACTATTGTATATAACTGGGGAAGAAGGTGAAAAATATTTCCTTGATATAATGTCATGTCATAACCAACTGAAATCCAACAATTGGAAACTGATCCAGATATCTATGAAAAGGGAGCCCAAAATAAGTTTAACAAATAAATATTAAATAAACAATTAATTATAACAGTATAATACAATTAAAAAACTAATAATAAATTTTTAATGAACTCCATACATACTCAAAATATAGCAGCTCGAAACTCTAAATTCGTTAGGGAAGTGTGCAACGGAGGAAGGCTTAATGGGTGTTCTATTTTTTTCCCAATAGCACTTATATTATAAGTGCTGTGGTACTCTATTTCTAAAGTTAAAAAAGAGTTCATGTAATTAGGAGGAAAAAAATGGAAGTTGATTAATTTAACTCCCATGCAAAAGGCTAGTTTTGAATAAAATTAATAGATGCCAAAAGCTTGGATGAATTGAGCTAATGTATATTGTTATTCATACTCAAGCATATAAATCTGCATTCACTTCGTCAATTTAATTTTCTTCTAATTAGGATTATTCAAGAGATTGGCGTTGATAATTGTTAAAACAATAAAACCTAAAAATTAATTTATTCCTAATTAAATTAACCATTCAAAGGTAGCCCAATAACTGCTAAAACAAAAGTTGTCTGAGCAGGGACAGATTGGAAAGGGAAATGCACGGCCCCTTCCATCTCCCCTTTTCTCGTTTGCAGATACAAACCTCCTGGGTGAAAGGCGATATTCCTATTCTAGCAGCGGAATTTCTGCTTCAGATATAAAAGTCTAACTTTTCACACCCGGCTTGTGCGCTTTTGCTTTAGGAAACTCCACCAACGACAAAATCTTTATCAACTATTTAGAGGAGCACAAGCTTTTCACCTTTACCAACTCAGTAAAGCTCCAAAGTTCATCTGTTTCAACGGATGGCCCCCTAAATATTATTTAACTATTATAAATTAATATTCATGTAATATTTATAATCATATATTTTTTGACCCCTCATATGATATAGTTTTGGATCTGTCCGTGTTTGAGTGCAAACTATTCTGATGGTTATCCATACAACTTTAGTAATACTGACTGGAATGATATGCCATGATATGCCATGTCATAGAAGTAGAATGACATGGGACAAGGGTAGGTTACACACATGCGTGTCCCCCCTCCCCTGGGTTCGTCTCTGCCGGAAAAATAGAAGCAGAGGTCTTGCACATCATTGATGTTCCCTCGAATATATTTTCTGTGAATGCTGCTGGCATAACTAGAACATGGGAGGATGTCTTTCATATGCGTATTTCCACAAAAGGGTGACTTGGTGCTAAAACTCCCGCATCCTTGTGGGTTTCAGGATAGAGTCAGACCCATTGTGAGTTTGATAAAGGGCAACCTAATCGTAGTGTGTTATAAAGCTCCCGCGGTTGGCTACGGGGAAGGCTTGCACACGCACAGGTCTGTTGCAGGTAATCTTTCCTGGCACCTAACCTTTGACCTCGTAATCAACACTACAATAACTCTTAACTATTGTACACAAACTTTCCTTCATTGTGCGTCTACTGTATATACAGGACTTGGAAATAGAAAAGAAAAATTATGGTTGAAAATGTAATGATTGTATGGTCTTTAAGTTGATGCATCATTTTTATTTATTAATTGTTGAAAATCTTGCAGGATACTAGTTGAGTGTTTGGATGATGTTGATGCGGAGTCACTTAAAAGTGCCGCAGAATATTTAATGGAAACATTAGCAGATCCAGCAGCTATTGTCCTGGGCTCATGTCCAGGTGAAGGGAAGGTTAGTCTAGTGGCTGCTTTTACTCCAGGGGTGGTTGGTCAGGGAATTCAGGCAGGTAAATTTATTGGACAGATAGCAAAATTATGTGGTGGTGGAGGAGGTGGAAGGTCCAACTTCGCTCAGGCTGGTGGGAGGAAGCCTGAAAATTTGGCTGGTGCCCTCGAGAAAGCTAAATCAGAGCTTATAGCTTATCTTTGTGAAAAAGGGAACTGA
Protein sequence

>MS.gene063074.t1

MNVTLAVAAAGGFISFKQLHLRRSRRLLPFCTSSSQDNDNNNCVSGDSIRERFLNFYASRGHKVLPSASLVPDDPTVLLTIAGMLQFKPIFLGKLPRQVPRATTSQRCIRTNDINNVGHTSRHHTFFEMLGNFSFGDYFKKQAIQWAWDLSTIEFGLPPERLWISVYQDDHETFQLWSQQVGVPVDRIKRLGEEDNFWTSGVTGPCGPCSEIYYDFYPERGYTDADLGDDTRFIEFYNLVFMQYNKKDDGSLEPLKQKNIDTGLGLERMARILQKAPNNYETDLILPIIEKASELANISYGISDDQTKRYLKIVGDHMRAIVFLISDGVVPSNVGRGYVVRRLIRRVVRTGRLLGIKGDGRGDPEGAFLPIIAQKVLELSTHIDADVKNRAPRILEELKREELRFVQTLERGEKLLEEKLVDAISSAERSGTVPCLAGEDVFILYDTYGFPMEITKEVAEERGVSIDMNGFDIEMEKQRRQSQAAHNTVKLAIGSGTSIADNVPDTEFIGYESLYCKAIVASLVVNGDPAVQVREGSNVEILLNKTPFYAESGGQIADHGFLYISEGENQPKAVVEIIDVQKSFGNIFVHKGTVQKGVLEVGKQVEATVDVKLRQRAKVHHTATHLLQAALKKVIGQETSQAGSLVAFDRLRFDFNFHRPLLDSELAEIELLINGWIEDAILLQTKVMPLADAKREGAIAMFGEKYGEEVRVVEVPGVSMELCGGTHVSNTSEIRGFKVISEQGIASGVRRIEAVAGEAFIEYVNARDLYLKQLCSTLKVKPEEVTVRIENLLEELRVARNENSAVRAKAAVYKASVIASRAFLVGHSKQYRILVECLDDVDAESLKSAAEYLMETLADPAAIVLGSCPGEGKVSLVAAFTPGVVGQGIQAGKFIGQIAKLCGGGGGGRSNFAQAGGRKPENLAGALEKAKSELIAYLCEKGN