Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0312820 | XP_003593594.1 | 87.500 | 256 | 13 | 5 | 1 | 241 | 1 | 252 | 2.42e-131 | 382 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0312820 | sp|Q5M750|VQ4_ARATH | 57.261 | 241 | 61 | 11 | 34 | 241 | 7 | 238 | 4.61e-65 | 206 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0006050 | Msa0312820 | -0.805913 | 1.061063e-49 | -8.615850e-47 |
| Msa0014960 | Msa0312820 | -0.803350 | 3.635609e-49 | -8.615850e-47 |
| Msa0021710 | Msa0312820 | -0.822645 | 2.120883e-53 | -8.615850e-47 |
| Msa0028050 | Msa0312820 | -0.813746 | 2.189644e-51 | -8.615850e-47 |
| Msa0028450 | Msa0312820 | -0.809263 | 2.063200e-50 | -8.615850e-47 |
| Msa0039590 | Msa0312820 | -0.825572 | 4.357101e-54 | -8.615850e-47 |
| Msa0046410 | Msa0312820 | -0.838972 | 2.092959e-57 | -8.615850e-47 |
| Msa0056160 | Msa0312820 | -0.822828 | 1.921844e-53 | -8.615850e-47 |
| Msa0094240 | Msa0312820 | -0.800212 | 1.602110e-48 | -8.615850e-47 |
| Msa0113910 | Msa0312820 | -0.806472 | 8.091739e-50 | -8.615850e-47 |
| Msa0115950 | Msa0312820 | -0.823019 | 1.735408e-53 | -8.615850e-47 |
| Msa0116260 | Msa0312820 | -0.828902 | 6.945305e-55 | -8.615850e-47 |
| Msa0116950 | Msa0312820 | -0.819294 | 1.252315e-52 | -8.615850e-47 |
| Msa0118250 | Msa0312820 | -0.810045 | 1.401250e-50 | -8.615850e-47 |
| Msa0127060 | Msa0312820 | -0.812716 | 3.685306e-51 | -8.615850e-47 |
| Msa0135400 | Msa0312820 | -0.803716 | 3.052200e-49 | -8.615850e-47 |
| Msa0160470 | Msa0312820 | -0.826910 | 2.092651e-54 | -8.615850e-47 |
| Msa0169320 | Msa0312820 | -0.802837 | 4.641411e-49 | -8.615850e-47 |
| Msa0170730 | Msa0312820 | -0.805081 | 1.585494e-49 | -8.615850e-47 |
| Msa0213020 | Msa0312820 | -0.830051 | 3.649902e-55 | -8.615850e-47 |
| Msa0222720 | Msa0312820 | -0.807056 | 6.089666e-50 | -8.615850e-47 |
| Msa0237440 | Msa0312820 | -0.844061 | 9.543987e-59 | -8.615850e-47 |
| Msa0264020 | Msa0312820 | -0.810688 | 1.017658e-50 | -8.615850e-47 |
| Msa0272420 | Msa0312820 | -0.807003 | 6.249530e-50 | -8.615850e-47 |
| Msa0292150 | Msa0312820 | -0.825616 | 4.254689e-54 | -8.615850e-47 |
| Msa0304020 | Msa0312820 | -0.802447 | 5.586253e-49 | -8.615850e-47 |
| Msa0312570 | Msa0312820 | -0.808428 | 3.112503e-50 | -8.615850e-47 |
| Msa0312820 | Msa0315010 | -0.800425 | 1.449849e-48 | -8.615850e-47 |
| Msa0312820 | Msa0319710 | -0.819299 | 1.249043e-52 | -8.615850e-47 |
| Msa0312820 | Msa0321230 | -0.814088 | 1.841122e-51 | -8.615850e-47 |
| Msa0312820 | Msa0327870 | -0.809192 | 2.136289e-50 | -8.615850e-47 |
| Msa0312820 | Msa0327900 | -0.826738 | 2.300657e-54 | -8.615850e-47 |
| Msa0312820 | Msa0330770 | -0.800939 | 1.138889e-48 | -8.615850e-47 |
| Msa0312820 | Msa0341710 | -0.802695 | 4.966351e-49 | -8.615850e-47 |
| Msa0312820 | Msa0367310 | -0.801821 | 7.513817e-49 | -8.615850e-47 |
| Msa0312820 | Msa0376480 | -0.838392 | 2.956177e-57 | -8.615850e-47 |
| Msa0312820 | Msa0379950 | -0.807417 | 5.107280e-50 | -8.615850e-47 |
| Msa0312820 | Msa0393140 | -0.822993 | 1.759885e-53 | -8.615850e-47 |
| Msa0312820 | Msa0400900 | -0.806550 | 7.790555e-50 | -8.615850e-47 |
| Msa0312820 | Msa0423590 | -0.809516 | 1.820495e-50 | -8.615850e-47 |
| Msa0312820 | Msa0424220 | -0.804461 | 2.136793e-49 | -8.615850e-47 |
| Msa0312820 | Msa0460220 | -0.808007 | 3.826539e-50 | -8.615850e-47 |
| Msa0312820 | Msa0463580 | -0.808209 | 3.466215e-50 | -8.615850e-47 |
| Msa0312820 | Msa0470650 | -0.802501 | 5.444105e-49 | -8.615850e-47 |
| Msa0312820 | Msa0471890 | -0.815627 | 8.391668e-52 | -8.615850e-47 |
| Msa0312820 | Msa0476820 | -0.819318 | 1.236692e-52 | -8.615850e-47 |
| Msa0312820 | Msa0481050 | -0.821418 | 4.081085e-53 | -8.615850e-47 |
| Msa0312820 | Msa0495510 | -0.805064 | 1.598874e-49 | -8.615850e-47 |
| Msa0312820 | Msa0500490 | -0.829056 | 6.370738e-55 | -8.615850e-47 |
| Msa0312820 | Msa0515680 | -0.805205 | 1.493811e-49 | -8.615850e-47 |
| Msa0312820 | Msa0528170 | -0.801041 | 1.085405e-48 | -8.615850e-47 |
| Msa0312820 | Msa0530250 | -0.803533 | 3.332223e-49 | -8.615850e-47 |
| Msa0312820 | Msa0533140 | -0.812954 | 3.269404e-51 | -8.615850e-47 |
| Msa0312820 | Msa0540160 | -0.820129 | 8.072301e-53 | -8.615850e-47 |
| Msa0312820 | Msa0558150 | -0.802425 | 5.643769e-49 | -8.615850e-47 |
| Msa0312820 | Msa0565150 | -0.823760 | 1.164205e-53 | -8.615850e-47 |
| Msa0312820 | Msa0565530 | -0.825564 | 4.376095e-54 | -8.615850e-47 |
| Msa0312820 | Msa0573970 | -0.808501 | 3.002914e-50 | -8.615850e-47 |
| Msa0312820 | Msa0576640 | -0.819546 | 1.097440e-52 | -8.615850e-47 |
| Msa0312820 | Msa0581190 | -0.822589 | 2.184768e-53 | -8.615850e-47 |
| Msa0312820 | Msa0589540 | -0.805959 | 1.037876e-49 | -8.615850e-47 |
| Msa0312820 | Msa0626900 | -0.807663 | 4.527311e-50 | -8.615850e-47 |
| Msa0312820 | Msa0635350 | -0.808178 | 3.519257e-50 | -8.615850e-47 |
| Msa0312820 | Msa0638040 | -0.807879 | 4.074205e-50 | -8.615850e-47 |
| Msa0312820 | Msa0660070 | -0.835735 | 1.411106e-56 | -8.615850e-47 |
| Msa0312820 | Msa0668000 | -0.838114 | 3.484869e-57 | -8.615850e-47 |
| Msa0312820 | Msa0672350 | -0.816941 | 4.266043e-52 | -8.615850e-47 |
| Msa0312820 | Msa0677110 | -0.804888 | 1.739791e-49 | -8.615850e-47 |
| Msa0312820 | Msa0688760 | -0.804820 | 1.797827e-49 | -8.615850e-47 |
| Msa0312820 | Msa0705710 | -0.807822 | 4.189360e-50 | -8.615850e-47 |
| Msa0312820 | Msa0705810 | -0.800520 | 1.386772e-48 | -8.615850e-47 |
| Msa0312820 | Msa0706130 | -0.817218 | 3.696711e-52 | -8.615850e-47 |
| Msa0312820 | Msa0707270 | -0.801002 | 1.105584e-48 | -8.615850e-47 |
| Msa0312820 | Msa0708380 | -0.803260 | 3.794397e-49 | -8.615850e-47 |
| Msa0312820 | Msa0715180 | -0.809282 | 2.044046e-50 | -8.615850e-47 |
| Msa0312820 | Msa0716670 | -0.806108 | 9.654520e-50 | -8.615850e-47 |
| Msa0312820 | Msa0720850 | -0.803105 | 4.085113e-49 | -8.615850e-47 |
| Msa0312820 | Msa0734760 | -0.826968 | 2.027563e-54 | -8.615850e-47 |
| Msa0312820 | Msa0752830 | -0.826741 | 2.296633e-54 | -8.615850e-47 |
| Msa0312820 | Msa0756440 | -0.812663 | 3.784850e-51 | -8.615850e-47 |
| Msa0312820 | Msa0772400 | -0.825399 | 4.788295e-54 | -8.615850e-47 |
| Msa0312820 | Msa0813630 | -0.834412 | 3.042868e-56 | -8.615850e-47 |
| Msa0312820 | Msa0821030 | -0.820168 | 7.908431e-53 | -8.615850e-47 |
| Msa0312820 | Msa0829110 | -0.814951 | 1.186270e-51 | -8.615850e-47 |
| Msa0312820 | Msa0829430 | -0.811828 | 5.758161e-51 | -8.615850e-47 |
| Msa0312820 | Msa0835550 | -0.805988 | 1.023068e-49 | -8.615850e-47 |
| Msa0312820 | Msa0836200 | -0.802295 | 6.002375e-49 | -8.615850e-47 |
| Msa0312820 | Msa0837590 | -0.807781 | 4.273605e-50 | -8.615850e-47 |
| Msa0312820 | Msa0868040 | -0.813187 | 2.905616e-51 | -8.615850e-47 |
| Msa0312820 | Msa0905170 | -0.817594 | 3.040459e-52 | -8.615850e-47 |
| Msa0312820 | Msa0932040 | -0.801065 | 1.073205e-48 | -8.615850e-47 |
| Msa0312820 | Msa0967670 | -0.827196 | 1.787730e-54 | -8.615850e-47 |
| Msa0312820 | Msa0970160 | -0.800464 | 1.423413e-48 | -8.615850e-47 |
| Msa0312820 | Msa0985570 | -0.808233 | 3.425138e-50 | -8.615850e-47 |
| Msa0312820 | Msa1044790 | -0.848065 | 7.773126e-60 | -8.615850e-47 |
| Msa0312820 | Msa1109500 | -0.831822 | 1.342359e-55 | -8.615850e-47 |
| Msa0312820 | Msa1126910 | -0.802337 | 5.885948e-49 | -8.615850e-47 |
| Msa0312820 | Msa1126980 | -0.804520 | 2.077091e-49 | -8.615850e-47 |
| Msa0312820 | Msa1144420 | -0.825373 | 4.855276e-54 | -8.615850e-47 |
| Msa0312820 | Msa1145070 | -0.804502 | 2.094751e-49 | -8.615850e-47 |
| Msa0312820 | Msa1149570 | -0.806336 | 8.642713e-50 | -8.615850e-47 |
| Msa0312820 | Msa1166020 | -0.820735 | 5.861575e-53 | -8.615850e-47 |
| Msa0312820 | Msa1199690 | -0.805318 | 1.414345e-49 | -8.615850e-47 |
| Msa0312820 | Msa1203840 | -0.823796 | 1.142266e-53 | -8.615850e-47 |
| Msa0312820 | Msa1203860 | -0.814081 | 1.846927e-51 | -8.615850e-47 |
| Msa0312820 | Msa1209790 | -0.804163 | 2.465152e-49 | -8.615850e-47 |
| Msa0312820 | Msa1221540 | -0.808852 | 2.525865e-50 | -8.615850e-47 |
| Msa0312820 | Msa1224310 | -0.823617 | 1.258007e-53 | -8.615850e-47 |
| Msa0312820 | Msa1279240 | -0.805149 | 1.534243e-49 | -8.615850e-47 |
| Msa0312820 | Msa1296270 | -0.802727 | 4.889589e-49 | -8.615850e-47 |
| Msa0312820 | Msa1297720 | -0.831028 | 2.105626e-55 | -8.615850e-47 |
| Msa0312820 | Msa1330910 | -0.813217 | 2.861564e-51 | -8.615850e-47 |
| Msa0312820 | Msa1334020 | -0.804586 | 2.011796e-49 | -8.615850e-47 |
| Msa0312820 | Msa1345490 | -0.809096 | 2.240538e-50 | -8.615850e-47 |
| Msa0312820 | Msa1349490 | -0.806613 | 7.555754e-50 | -8.615850e-47 |
| Msa0312820 | Msa1349640 | -0.810998 | 8.720010e-51 | -8.615850e-47 |
| Msa0312820 | Msa1366700 | -0.803226 | 3.857685e-49 | -8.615850e-47 |
| Msa0312820 | Msa1368800 | -0.813328 | 2.706550e-51 | -8.615850e-47 |
| Msa0312820 | Msa1369180 | -0.801013 | 1.100182e-48 | -8.615850e-47 |
| Msa0312820 | Msa1378840 | -0.813049 | 3.115179e-51 | -8.615850e-47 |
| Msa0312820 | Msa1384980 | -0.830175 | 3.405358e-55 | -8.615850e-47 |
| Msa0312820 | Msa1386020 | -0.805421 | 1.345988e-49 | -8.615850e-47 |
| Msa0312820 | Msa1390870 | 0.945107 | 5.666106e-104 | -8.615850e-47 |
| Msa0312820 | Msa1396310 | -0.819155 | 1.347557e-52 | -8.615850e-47 |
| Msa0312820 | Msa1397110 | -0.812323 | 4.491300e-51 | -8.615850e-47 |
| Msa0312820 | Msa1403900 | -0.802620 | 5.144710e-49 | -8.615850e-47 |
| Msa0312820 | Msa1414000 | -0.803130 | 4.037685e-49 | -8.615850e-47 |
| Msa0312820 | Msa1418010 | -0.819052 | 1.422093e-52 | -8.615850e-47 |
| Msa0312820 | Msa1432350 | -0.806313 | 8.741561e-50 | -8.615850e-47 |
| Msa0312820 | Msa1450420 | -0.803054 | 4.185376e-49 | -8.615850e-47 |
| Msa0312820 | Msa1456620 | -0.802982 | 4.332365e-49 | -8.615850e-47 |
| Msa0312820 | Msa1463500 | -0.846797 | 1.732868e-59 | -8.615850e-47 |
| Msa0312820 | Msa1465380 | -0.825739 | 3.978230e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0312820 | MtrunA17_Chr2g0282361 | 87.500 | 256 | 13 | 5 | 1 | 241 | 1 | 252 | 2.23e-135 | 382 |
| Msa0312820 | MtrunA17_Chr4g0047141 | 68.205 | 195 | 48 | 9 | 65 | 248 | 50 | 241 | 3.27e-64 | 200 |
| Msa0312820 | MtrunA17_Chr2g0317321 | 56.716 | 201 | 59 | 7 | 61 | 242 | 51 | 242 | 2.00e-53 | 173 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0312820 | AT1G28280.2 | 57.438 | 242 | 61 | 11 | 34 | 242 | 7 | 239 | 1.10e-66 | 207 |
| Msa0312820 | AT1G28280.1 | 57.261 | 241 | 61 | 11 | 34 | 241 | 7 | 238 | 4.69e-66 | 206 |
| Msa0312820 | AT3G15300.1 | 50.249 | 201 | 64 | 12 | 65 | 242 | 29 | 216 | 1.89e-38 | 134 |
| Msa0312820 | AT5G53830.1 | 52.970 | 202 | 54 | 12 | 72 | 239 | 45 | 239 | 3.30e-37 | 131 |
| Msa0312820 | AT2G33780.1 | 45.026 | 191 | 78 | 7 | 66 | 245 | 26 | 200 | 7.07e-34 | 122 |
| Msa0312820 | AT1G80450.1 | 36.842 | 171 | 84 | 8 | 76 | 242 | 15 | 165 | 7.26e-14 | 68.2 |
| Msa0312820 | AT5G08480.2 | 35.673 | 171 | 91 | 6 | 76 | 243 | 17 | 171 | 8.57e-11 | 59.7 |
| Msa0312820 | AT5G08480.3 | 35.673 | 171 | 91 | 6 | 76 | 243 | 17 | 171 | 8.57e-11 | 59.7 |
Find 62 sgRNAs with CRISPR-Local
Find 148 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAACAAATGTTGTTGGATA+TGG | 0.219375 | 2_4:+8005518 | None:intergenic |
| GAAGAACCGGATCGGTTAAA+TGG | 0.240342 | 2_4:+8005191 | None:intergenic |
| GAAACTTGAGTTGGAGAAAA+TGG | 0.246762 | 2_4:+8005304 | None:intergenic |
| ACCTGGTTCGGATCTTGTTA+TGG | 0.250404 | 2_4:+8005544 | None:intergenic |
| CCTGGTTCGGATCTTGTTAT+GGG | 0.271576 | 2_4:+8005545 | None:intergenic |
| TGCTAGAAATGGTTCTTCTT+TGG | 0.294813 | 2_4:-8005162 | Msa0312820:CDS |
| CAAGTTGTTCAAATGTTAAC+TGG | 0.300641 | 2_4:-8005473 | Msa0312820:CDS |
| TGGGTCTTGGTGAAGGAATT+TGG | 0.302573 | 2_4:+8005618 | None:intergenic |
| ACTACTTCTCCTAGGGCTTC+AGG | 0.309005 | 2_4:-8005029 | Msa0312820:CDS |
| GAGAAAATGGGTGGTAATAA+AGG | 0.316316 | 2_4:+8005317 | None:intergenic |
| TTGTGTCCATTACTTGTTAT+GGG | 0.319171 | 2_4:+8005599 | None:intergenic |
| GTTGTGTCCATTACTTGTTA+TGG | 0.323237 | 2_4:+8005598 | None:intergenic |
| TCGGGTATGAGTGGTGTAAC+CGG | 0.325510 | 2_4:+8005215 | None:intergenic |
| CCATTACTTGTTATGGGTCT+TGG | 0.332135 | 2_4:+8005605 | None:intergenic |
| TGGGTCTTGGTGATTGAATT+TGG | 0.337028 | 2_4:+8005564 | None:intergenic |
| GTAATAAAGGGTTAATGTTA+AGG | 0.349071 | 2_4:+8005330 | None:intergenic |
| TCGGATCTTGTTATGGGTCT+TGG | 0.376668 | 2_4:+8005551 | None:intergenic |
| TTGGTGGAATGTTATGGTTA+TGG | 0.382884 | 2_4:+8005405 | None:intergenic |
| TCTTCTTCTGCTGCTAGAAA+TGG | 0.396481 | 2_4:-8005173 | Msa0312820:CDS |
| GATAAAGCTATAAGAGAAAA+GGG | 0.402366 | 2_4:-8005125 | Msa0312820:CDS |
| TTGGTGATTGAATTTGGTTA+TGG | 0.403350 | 2_4:+8005570 | None:intergenic |
| GTCTCTAGGAGTTGAAGCTC+TGG | 0.403528 | 2_4:+8005082 | None:intergenic |
| ATTGAATTTGGTTATGGCTA+TGG | 0.404356 | 2_4:+8005576 | None:intergenic |
| TGGATATGGGTTACCTGGTT+CGG | 0.412484 | 2_4:+8005532 | None:intergenic |
| TTGGTGAAGGAATTTGGTTA+TGG | 0.445585 | 2_4:+8005624 | None:intergenic |
| AGATAAAGCTATAAGAGAAA+AGG | 0.449288 | 2_4:-8005126 | Msa0312820:CDS |
| GAACAAATGTTGTTGGATAT+GGG | 0.451854 | 2_4:+8005519 | None:intergenic |
| GAGCAAGAGAAGGAAAATCT+AGG | 0.454989 | 2_4:+8005243 | None:intergenic |
| TCCTTCTCTTGCTCTTAGCC+CGG | 0.462751 | 2_4:-8005234 | Msa0312820:CDS |
| GGAATGTTATGGTTATGGTT+TGG | 0.475070 | 2_4:+8005410 | None:intergenic |
| TCAGCTTGAACAAATGTTGT+TGG | 0.479156 | 2_4:+8005512 | None:intergenic |
| CCGGATCGGTTAAATGGGTC+GGG | 0.490143 | 2_4:+8005197 | None:intergenic |
| AGAAAATGGGTGGTAATAAA+GGG | 0.497203 | 2_4:+8005318 | None:intergenic |
| TCTAGCAGCAGAAGAAGAAC+CGG | 0.502830 | 2_4:+8005178 | None:intergenic |
| CCCGACCCATTTAACCGATC+CGG | 0.503223 | 2_4:-8005197 | Msa0312820:CDS |
| AAGAACCGGATCGGTTAAAT+GGG | 0.503269 | 2_4:+8005192 | None:intergenic |
| CCAAGACCCATAACAAGTAA+TGG | 0.503842 | 2_4:-8005605 | Msa0312820:CDS |
| CTTGTTATGGGTCTTGGTGA+AGG | 0.506753 | 2_4:+8005611 | None:intergenic |
| AAGGAATTTGGTTATGGCTA+TGG | 0.513007 | 2_4:+8005630 | None:intergenic |
| TCAAATGTTAACTGGTTCCT+CGG | 0.513372 | 2_4:-8005465 | Msa0312820:CDS |
| GGAGTTGAAGCTCTGGGAGA+AGG | 0.513871 | 2_4:+8005089 | None:intergenic |
| GAAGGAAAATCTAGGATACT+AGG | 0.515704 | 2_4:+8005251 | None:intergenic |
| CGGGTATGAGTGGTGTAACC+GGG | 0.519254 | 2_4:+8005216 | None:intergenic |
| ACCGGATCGGTTAAATGGGT+CGG | 0.532624 | 2_4:+8005196 | None:intergenic |
| CATTGATGATGTGGAATCCA+TGG | 0.533309 | 2_4:+8005736 | None:intergenic |
| AAACTTGAGTTGGAGAAAAT+GGG | 0.542299 | 2_4:+8005305 | None:intergenic |
| GTTGTTGGATATGGGTTACC+TGG | 0.543727 | 2_4:+8005527 | None:intergenic |
| TTGGTATCTCATTGATGATG+TGG | 0.551120 | 2_4:+8005727 | None:intergenic |
| GAAGCCCTAGGAGAAGTAGT+AGG | 0.571572 | 2_4:+8005032 | None:intergenic |
| CAAACTCAAACTCAAAGCAA+TGG | 0.576732 | 2_4:-8005659 | Msa0312820:CDS |
| GATGAAGGACCTGAAGCCCT+AGG | 0.577284 | 2_4:+8005020 | None:intergenic |
| TCTCTAGGAGTTGAAGCTCT+GGG | 0.580776 | 2_4:+8005083 | None:intergenic |
| AAACGAGGTTCAGAGTCTCT+AGG | 0.581466 | 2_4:+8005068 | None:intergenic |
| ACGAGGTGAGAAACTTGAGT+TGG | 0.589923 | 2_4:+8005295 | None:intergenic |
| CCCATAACAAGATCCGAACC+AGG | 0.590432 | 2_4:-8005545 | Msa0312820:CDS |
| TTAAATGGGTCGGGTATGAG+TGG | 0.616779 | 2_4:+8005206 | None:intergenic |
| AAGATCTCTTGTTTGTTACG+AGG | 0.644449 | 2_4:+8005278 | None:intergenic |
| TTGAACAACTTGTTTGAACG+AGG | 0.648447 | 2_4:+8005484 | None:intergenic |
| CAGCAGAAGAAGAACCGGAT+CGG | 0.652832 | 2_4:+8005183 | None:intergenic |
| ACCGGGCTAAGAGCAAGAGA+AGG | 0.662342 | 2_4:+8005233 | None:intergenic |
| CTTGAGTTGGAGAAAATGGG+TGG | 0.666617 | 2_4:+8005308 | None:intergenic |
| ATCATCAATGAGATACCAAG+AGG | 0.685459 | 2_4:-8005723 | Msa0312820:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGAAACCTTGTTGTTTTTT+TGG | + | chr2_4:8005346-8005365 | None:intergenic | 25.0% |
| ! | GTAATAAAGGGTTAATGTTA+AGG | + | chr2_4:8005402-8005421 | None:intergenic | 25.0% |
| ! | AGATAAAGCTATAAGAGAAA+AGG | - | chr2_4:8005603-8005622 | Msa0312820:CDS | 25.0% |
| ! | GATAAAGCTATAAGAGAAAA+GGG | - | chr2_4:8005604-8005623 | Msa0312820:CDS | 25.0% |
| !!! | GAATATTTTGAAGAAGATGA+AGG | + | chr2_4:8005727-8005746 | None:intergenic | 25.0% |
| !!! | TTGAAACCTTGTTGTTTTTT+TGG | + | chr2_4:8005346-8005365 | None:intergenic | 25.0% |
| ! | GTAATAAAGGGTTAATGTTA+AGG | + | chr2_4:8005402-8005421 | None:intergenic | 25.0% |
| ! | AGATAAAGCTATAAGAGAAA+AGG | - | chr2_4:8005603-8005622 | Msa0312820:CDS | 25.0% |
| ! | GATAAAGCTATAAGAGAAAA+GGG | - | chr2_4:8005604-8005623 | Msa0312820:CDS | 25.0% |
| !!! | GAATATTTTGAAGAAGATGA+AGG | + | chr2_4:8005727-8005746 | None:intergenic | 25.0% |
| !! | TGAGTTTGAGTTTGAGTTTT+TGG | + | chr2_4:8005064-8005083 | None:intergenic | 30.0% |
| !! | TTGTGTCCATTACTTGTTAT+GGG | + | chr2_4:8005133-8005152 | None:intergenic | 30.0% |
| ATTGAATTTGGTTATGGCTA+TGG | + | chr2_4:8005156-8005175 | None:intergenic | 30.0% | |
| ! | TTGGTGATTGAATTTGGTTA+TGG | + | chr2_4:8005162-8005181 | None:intergenic | 30.0% |
| GAACAAATGTTGTTGGATAT+GGG | + | chr2_4:8005213-8005232 | None:intergenic | 30.0% | |
| TGAACAAATGTTGTTGGATA+TGG | + | chr2_4:8005214-8005233 | None:intergenic | 30.0% | |
| CAAGTTGTTCAAATGTTAAC+TGG | - | chr2_4:8005256-8005275 | Msa0312820:CDS | 30.0% | |
| !!! | GTTTTTTTGGTGGAATGTTA+TGG | + | chr2_4:8005333-8005352 | None:intergenic | 30.0% |
| ATTCCACCAAAAAAACAACA+AGG | - | chr2_4:8005337-8005356 | Msa0312820:CDS | 30.0% | |
| !!! | AAACCTTGTTGTTTTTTTGG+TGG | + | chr2_4:8005343-8005362 | None:intergenic | 30.0% |
| AGAAAATGGGTGGTAATAAA+GGG | + | chr2_4:8005414-8005433 | None:intergenic | 30.0% | |
| !! | AAACTTGAGTTGGAGAAAAT+GGG | + | chr2_4:8005427-8005446 | None:intergenic | 30.0% |
| !! | TGAGTTTGAGTTTGAGTTTT+TGG | + | chr2_4:8005064-8005083 | None:intergenic | 30.0% |
| !! | TTGTGTCCATTACTTGTTAT+GGG | + | chr2_4:8005133-8005152 | None:intergenic | 30.0% |
| ATTGAATTTGGTTATGGCTA+TGG | + | chr2_4:8005156-8005175 | None:intergenic | 30.0% | |
| ! | TTGGTGATTGAATTTGGTTA+TGG | + | chr2_4:8005162-8005181 | None:intergenic | 30.0% |
| GAACAAATGTTGTTGGATAT+GGG | + | chr2_4:8005213-8005232 | None:intergenic | 30.0% | |
| TGAACAAATGTTGTTGGATA+TGG | + | chr2_4:8005214-8005233 | None:intergenic | 30.0% | |
| CAAGTTGTTCAAATGTTAAC+TGG | - | chr2_4:8005256-8005275 | Msa0312820:CDS | 30.0% | |
| !!! | GTTTTTTTGGTGGAATGTTA+TGG | + | chr2_4:8005333-8005352 | None:intergenic | 30.0% |
| ATTCCACCAAAAAAACAACA+AGG | - | chr2_4:8005337-8005356 | Msa0312820:CDS | 30.0% | |
| !!! | AAACCTTGTTGTTTTTTTGG+TGG | + | chr2_4:8005343-8005362 | None:intergenic | 30.0% |
| AGAAAATGGGTGGTAATAAA+GGG | + | chr2_4:8005414-8005433 | None:intergenic | 30.0% | |
| !! | AAACTTGAGTTGGAGAAAAT+GGG | + | chr2_4:8005427-8005446 | None:intergenic | 30.0% |
| !! | TTGGTATCTCATTGATGATG+TGG | + | chr2_4:8005005-8005024 | None:intergenic | 35.0% |
| ATCATCAATGAGATACCAAG+AGG | - | chr2_4:8005006-8005025 | Msa0312820:CDS | 35.0% | |
| ! | GAGATGATTTTTGTTCCTCT+TGG | + | chr2_4:8005024-8005043 | None:intergenic | 35.0% |
| !!! | TGAGTTTGAGTTTTTGGTGA+AGG | + | chr2_4:8005058-8005077 | None:intergenic | 35.0% |
| CAAACTCAAACTCAAAGCAA+TGG | - | chr2_4:8005070-8005089 | Msa0312820:CDS | 35.0% | |
| AAGGAATTTGGTTATGGCTA+TGG | + | chr2_4:8005102-8005121 | None:intergenic | 35.0% | |
| TTGGTGAAGGAATTTGGTTA+TGG | + | chr2_4:8005108-8005127 | None:intergenic | 35.0% | |
| ! | GTTGTGTCCATTACTTGTTA+TGG | + | chr2_4:8005134-8005153 | None:intergenic | 35.0% |
| TCAGCTTGAACAAATGTTGT+TGG | + | chr2_4:8005220-8005239 | None:intergenic | 35.0% | |
| ! | TTGAACAACTTGTTTGAACG+AGG | + | chr2_4:8005248-8005267 | None:intergenic | 35.0% |
| ! | TCAAATGTTAACTGGTTCCT+CGG | - | chr2_4:8005264-8005283 | Msa0312820:CDS | 35.0% |
| GGAATGTTATGGTTATGGTT+TGG | + | chr2_4:8005322-8005341 | None:intergenic | 35.0% | |
| TTGGTGGAATGTTATGGTTA+TGG | + | chr2_4:8005327-8005346 | None:intergenic | 35.0% | |
| GAGAAAATGGGTGGTAATAA+AGG | + | chr2_4:8005415-8005434 | None:intergenic | 35.0% | |
| !! | GAAACTTGAGTTGGAGAAAA+TGG | + | chr2_4:8005428-8005447 | None:intergenic | 35.0% |
| ! | AAGATCTCTTGTTTGTTACG+AGG | + | chr2_4:8005454-8005473 | None:intergenic | 35.0% |
| ! | GAAGGAAAATCTAGGATACT+AGG | + | chr2_4:8005481-8005500 | None:intergenic | 35.0% |
| TGCTAGAAATGGTTCTTCTT+TGG | - | chr2_4:8005567-8005586 | Msa0312820:CDS | 35.0% | |
| ! | TTTTTCCTACTACTTCTCCT+AGG | - | chr2_4:8005692-8005711 | Msa0312820:CDS | 35.0% |
| ! | TTTTCCTACTACTTCTCCTA+GGG | - | chr2_4:8005693-8005712 | Msa0312820:CDS | 35.0% |
| !! | TTGGTATCTCATTGATGATG+TGG | + | chr2_4:8005005-8005024 | None:intergenic | 35.0% |
| ATCATCAATGAGATACCAAG+AGG | - | chr2_4:8005006-8005025 | Msa0312820:CDS | 35.0% | |
| ! | GAGATGATTTTTGTTCCTCT+TGG | + | chr2_4:8005024-8005043 | None:intergenic | 35.0% |
| !!! | TGAGTTTGAGTTTTTGGTGA+AGG | + | chr2_4:8005058-8005077 | None:intergenic | 35.0% |
| CAAACTCAAACTCAAAGCAA+TGG | - | chr2_4:8005070-8005089 | Msa0312820:CDS | 35.0% | |
| AAGGAATTTGGTTATGGCTA+TGG | + | chr2_4:8005102-8005121 | None:intergenic | 35.0% | |
| TTGGTGAAGGAATTTGGTTA+TGG | + | chr2_4:8005108-8005127 | None:intergenic | 35.0% | |
| ! | GTTGTGTCCATTACTTGTTA+TGG | + | chr2_4:8005134-8005153 | None:intergenic | 35.0% |
| TCAGCTTGAACAAATGTTGT+TGG | + | chr2_4:8005220-8005239 | None:intergenic | 35.0% | |
| ! | TTGAACAACTTGTTTGAACG+AGG | + | chr2_4:8005248-8005267 | None:intergenic | 35.0% |
| ! | TCAAATGTTAACTGGTTCCT+CGG | - | chr2_4:8005264-8005283 | Msa0312820:CDS | 35.0% |
| GGAATGTTATGGTTATGGTT+TGG | + | chr2_4:8005322-8005341 | None:intergenic | 35.0% | |
| TTGGTGGAATGTTATGGTTA+TGG | + | chr2_4:8005327-8005346 | None:intergenic | 35.0% | |
| GAGAAAATGGGTGGTAATAA+AGG | + | chr2_4:8005415-8005434 | None:intergenic | 35.0% | |
| !! | GAAACTTGAGTTGGAGAAAA+TGG | + | chr2_4:8005428-8005447 | None:intergenic | 35.0% |
| ! | AAGATCTCTTGTTTGTTACG+AGG | + | chr2_4:8005454-8005473 | None:intergenic | 35.0% |
| ! | GAAGGAAAATCTAGGATACT+AGG | + | chr2_4:8005481-8005500 | None:intergenic | 35.0% |
| TGCTAGAAATGGTTCTTCTT+TGG | - | chr2_4:8005567-8005586 | Msa0312820:CDS | 35.0% | |
| ! | TTTTTCCTACTACTTCTCCT+AGG | - | chr2_4:8005692-8005711 | Msa0312820:CDS | 35.0% |
| ! | TTTTCCTACTACTTCTCCTA+GGG | - | chr2_4:8005693-8005712 | Msa0312820:CDS | 35.0% |
| !! | CCATTACTTGTTATGGGTCT+TGG | + | chr2_4:8005127-8005146 | None:intergenic | 40.0% |
| CCAAGACCCATAACAAGTAA+TGG | - | chr2_4:8005124-8005143 | Msa0312820:CDS | 40.0% | |
| ! | TGGGTCTTGGTGATTGAATT+TGG | + | chr2_4:8005168-8005187 | None:intergenic | 40.0% |
| GAGCAAGAGAAGGAAAATCT+AGG | + | chr2_4:8005489-8005508 | None:intergenic | 40.0% | |
| AAGAACCGGATCGGTTAAAT+GGG | + | chr2_4:8005540-8005559 | None:intergenic | 40.0% | |
| TCTTCTTCTGCTGCTAGAAA+TGG | - | chr2_4:8005556-8005575 | Msa0312820:CDS | 40.0% | |
| GGAGAAGTAGTAGGAAAAAG+AGG | + | chr2_4:8005691-8005710 | None:intergenic | 40.0% | |
| !! | CCATTACTTGTTATGGGTCT+TGG | + | chr2_4:8005127-8005146 | None:intergenic | 40.0% |
| CCAAGACCCATAACAAGTAA+TGG | - | chr2_4:8005124-8005143 | Msa0312820:CDS | 40.0% | |
| ! | TGGGTCTTGGTGATTGAATT+TGG | + | chr2_4:8005168-8005187 | None:intergenic | 40.0% |
| GAGCAAGAGAAGGAAAATCT+AGG | + | chr2_4:8005489-8005508 | None:intergenic | 40.0% | |
| AAGAACCGGATCGGTTAAAT+GGG | + | chr2_4:8005540-8005559 | None:intergenic | 40.0% | |
| TCTTCTTCTGCTGCTAGAAA+TGG | - | chr2_4:8005556-8005575 | Msa0312820:CDS | 40.0% | |
| GGAGAAGTAGTAGGAAAAAG+AGG | + | chr2_4:8005691-8005710 | None:intergenic | 40.0% | |
| TGGGTCTTGGTGAAGGAATT+TGG | + | chr2_4:8005114-8005133 | None:intergenic | 45.0% | |
| ! | CTTGTTATGGGTCTTGGTGA+AGG | + | chr2_4:8005121-8005140 | None:intergenic | 45.0% |
| ! | TCGGATCTTGTTATGGGTCT+TGG | + | chr2_4:8005181-8005200 | None:intergenic | 45.0% |
| !! | CCTGGTTCGGATCTTGTTAT+GGG | + | chr2_4:8005187-8005206 | None:intergenic | 45.0% |
| !! | ACCTGGTTCGGATCTTGTTA+TGG | + | chr2_4:8005188-8005207 | None:intergenic | 45.0% |
| ! | TGGATATGGGTTACCTGGTT+CGG | + | chr2_4:8005200-8005219 | None:intergenic | 45.0% |
| ! | GTTGTTGGATATGGGTTACC+TGG | + | chr2_4:8005205-8005224 | None:intergenic | 45.0% |
| !! | CTTGAGTTGGAGAAAATGGG+TGG | + | chr2_4:8005424-8005443 | None:intergenic | 45.0% |
| ACGAGGTGAGAAACTTGAGT+TGG | + | chr2_4:8005437-8005456 | None:intergenic | 45.0% | |
| TTAAATGGGTCGGGTATGAG+TGG | + | chr2_4:8005526-8005545 | None:intergenic | 45.0% | |
| GAAGAACCGGATCGGTTAAA+TGG | + | chr2_4:8005541-8005560 | None:intergenic | 45.0% | |
| TCTAGCAGCAGAAGAAGAAC+CGG | + | chr2_4:8005554-8005573 | None:intergenic | 45.0% | |
| ! | TCTCTAGGAGTTGAAGCTCT+GGG | + | chr2_4:8005649-8005668 | None:intergenic | 45.0% |
| AAACGAGGTTCAGAGTCTCT+AGG | + | chr2_4:8005664-8005683 | None:intergenic | 45.0% | |
| GGAAAAAGAGGAAGCAAACG+AGG | + | chr2_4:8005679-8005698 | None:intergenic | 45.0% | |
| TGGGTCTTGGTGAAGGAATT+TGG | + | chr2_4:8005114-8005133 | None:intergenic | 45.0% | |
| ! | CTTGTTATGGGTCTTGGTGA+AGG | + | chr2_4:8005121-8005140 | None:intergenic | 45.0% |
| ! | TCGGATCTTGTTATGGGTCT+TGG | + | chr2_4:8005181-8005200 | None:intergenic | 45.0% |
| !! | CCTGGTTCGGATCTTGTTAT+GGG | + | chr2_4:8005187-8005206 | None:intergenic | 45.0% |
| !! | ACCTGGTTCGGATCTTGTTA+TGG | + | chr2_4:8005188-8005207 | None:intergenic | 45.0% |
| ! | TGGATATGGGTTACCTGGTT+CGG | + | chr2_4:8005200-8005219 | None:intergenic | 45.0% |
| ! | GTTGTTGGATATGGGTTACC+TGG | + | chr2_4:8005205-8005224 | None:intergenic | 45.0% |
| !! | CTTGAGTTGGAGAAAATGGG+TGG | + | chr2_4:8005424-8005443 | None:intergenic | 45.0% |
| ACGAGGTGAGAAACTTGAGT+TGG | + | chr2_4:8005437-8005456 | None:intergenic | 45.0% | |
| TTAAATGGGTCGGGTATGAG+TGG | + | chr2_4:8005526-8005545 | None:intergenic | 45.0% | |
| GAAGAACCGGATCGGTTAAA+TGG | + | chr2_4:8005541-8005560 | None:intergenic | 45.0% | |
| TCTAGCAGCAGAAGAAGAAC+CGG | + | chr2_4:8005554-8005573 | None:intergenic | 45.0% | |
| ! | TCTCTAGGAGTTGAAGCTCT+GGG | + | chr2_4:8005649-8005668 | None:intergenic | 45.0% |
| AAACGAGGTTCAGAGTCTCT+AGG | + | chr2_4:8005664-8005683 | None:intergenic | 45.0% | |
| GGAAAAAGAGGAAGCAAACG+AGG | + | chr2_4:8005679-8005698 | None:intergenic | 45.0% | |
| CCCATAACAAGATCCGAACC+AGG | - | chr2_4:8005184-8005203 | Msa0312820:CDS | 50.0% | |
| !!! | AGCTTGTTTTGCTGTCTCCG+AGG | + | chr2_4:8005284-8005303 | None:intergenic | 50.0% |
| TCCTTCTCTTGCTCTTAGCC+CGG | - | chr2_4:8005495-8005514 | Msa0312820:CDS | 50.0% | |
| TCGGGTATGAGTGGTGTAAC+CGG | + | chr2_4:8005517-8005536 | None:intergenic | 50.0% | |
| ACCGGATCGGTTAAATGGGT+CGG | + | chr2_4:8005536-8005555 | None:intergenic | 50.0% | |
| CAGCAGAAGAAGAACCGGAT+CGG | + | chr2_4:8005549-8005568 | None:intergenic | 50.0% | |
| ! | GTCTCTAGGAGTTGAAGCTC+TGG | + | chr2_4:8005650-8005669 | None:intergenic | 50.0% |
| GAAGCCCTAGGAGAAGTAGT+AGG | + | chr2_4:8005700-8005719 | None:intergenic | 50.0% | |
| ACTACTTCTCCTAGGGCTTC+AGG | - | chr2_4:8005700-8005719 | Msa0312820:CDS | 50.0% | |
| CCCATAACAAGATCCGAACC+AGG | - | chr2_4:8005184-8005203 | Msa0312820:CDS | 50.0% | |
| !!! | AGCTTGTTTTGCTGTCTCCG+AGG | + | chr2_4:8005284-8005303 | None:intergenic | 50.0% |
| TCCTTCTCTTGCTCTTAGCC+CGG | - | chr2_4:8005495-8005514 | Msa0312820:CDS | 50.0% | |
| TCGGGTATGAGTGGTGTAAC+CGG | + | chr2_4:8005517-8005536 | None:intergenic | 50.0% | |
| ACCGGATCGGTTAAATGGGT+CGG | + | chr2_4:8005536-8005555 | None:intergenic | 50.0% | |
| CAGCAGAAGAAGAACCGGAT+CGG | + | chr2_4:8005549-8005568 | None:intergenic | 50.0% | |
| ! | GTCTCTAGGAGTTGAAGCTC+TGG | + | chr2_4:8005650-8005669 | None:intergenic | 50.0% |
| GAAGCCCTAGGAGAAGTAGT+AGG | + | chr2_4:8005700-8005719 | None:intergenic | 50.0% | |
| ACTACTTCTCCTAGGGCTTC+AGG | - | chr2_4:8005700-8005719 | Msa0312820:CDS | 50.0% | |
| ACCGGGCTAAGAGCAAGAGA+AGG | + | chr2_4:8005499-8005518 | None:intergenic | 55.0% | |
| CGGGTATGAGTGGTGTAACC+GGG | + | chr2_4:8005516-8005535 | None:intergenic | 55.0% | |
| CCGGATCGGTTAAATGGGTC+GGG | + | chr2_4:8005535-8005554 | None:intergenic | 55.0% | |
| CCCGACCCATTTAACCGATC+CGG | - | chr2_4:8005532-8005551 | Msa0312820:CDS | 55.0% | |
| !! | GGAGTTGAAGCTCTGGGAGA+AGG | + | chr2_4:8005643-8005662 | None:intergenic | 55.0% |
| GATGAAGGACCTGAAGCCCT+AGG | + | chr2_4:8005712-8005731 | None:intergenic | 55.0% | |
| ACCGGGCTAAGAGCAAGAGA+AGG | + | chr2_4:8005499-8005518 | None:intergenic | 55.0% | |
| CGGGTATGAGTGGTGTAACC+GGG | + | chr2_4:8005516-8005535 | None:intergenic | 55.0% | |
| CCGGATCGGTTAAATGGGTC+GGG | + | chr2_4:8005535-8005554 | None:intergenic | 55.0% | |
| CCCGACCCATTTAACCGATC+CGG | - | chr2_4:8005532-8005551 | Msa0312820:CDS | 55.0% | |
| !! | GGAGTTGAAGCTCTGGGAGA+AGG | + | chr2_4:8005643-8005662 | None:intergenic | 55.0% |
| GATGAAGGACCTGAAGCCCT+AGG | + | chr2_4:8005712-8005731 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 8004995 | 8005756 | 8004995 | ID=Msa0312820;Name=Msa0312820 |
| chr2_4 | mRNA | 8004995 | 8005756 | 8004995 | ID=Msa0312820-mRNA-1;Parent=Msa0312820;Name=Msa0312820-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|253 |
| chr2_4 | exon | 8004995 | 8005756 | 8004995 | ID=Msa0312820-mRNA-1:exon:3121;Parent=Msa0312820-mRNA-1 |
| chr2_4 | CDS | 8004995 | 8005756 | 8004995 | ID=Msa0312820-mRNA-1:cds;Parent=Msa0312820-mRNA-1 |
| Gene Sequence |
| Protein sequence |
