Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1390870 | XP_003593594.1 | 87.891 | 256 | 12 | 5 | 1 | 241 | 1 | 252 | 4.43e-132 | 384 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1390870 | sp|Q5M750|VQ4_ARATH | 57.261 | 241 | 61 | 11 | 34 | 241 | 7 | 238 | 4.51e-65 | 206 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1390870 | Q2HTS9 | 87.891 | 256 | 12 | 5 | 1 | 241 | 1 | 252 | 2.12e-132 | 384 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0021710 | Msa1390870 | -0.828218 | 1.016054e-54 | -8.615850e-47 |
| Msa0025340 | Msa1390870 | -0.822802 | 1.949689e-53 | -8.615850e-47 |
| Msa0039590 | Msa1390870 | -0.820945 | 5.244779e-53 | -8.615850e-47 |
| Msa0046700 | Msa1390870 | -0.810699 | 1.012499e-50 | -8.615850e-47 |
| Msa0052550 | Msa1390870 | -0.800801 | 1.215632e-48 | -8.615850e-47 |
| Msa0070580 | Msa1390870 | -0.821205 | 4.568547e-53 | -8.615850e-47 |
| Msa0078170 | Msa1390870 | -0.807491 | 4.925644e-50 | -8.615850e-47 |
| Msa0093830 | Msa1390870 | -0.810506 | 1.114561e-50 | -8.615850e-47 |
| Msa0098550 | Msa1390870 | -0.812935 | 3.300317e-51 | -8.615850e-47 |
| Msa0115950 | Msa1390870 | -0.818566 | 1.833059e-52 | -8.615850e-47 |
| Msa0116950 | Msa1390870 | -0.811777 | 5.909128e-51 | -8.615850e-47 |
| Msa1345490 | Msa1390870 | -0.818364 | 2.037717e-52 | -8.615850e-47 |
| Msa1349490 | Msa1390870 | -0.806306 | 8.771816e-50 | -8.615850e-47 |
| Msa1349640 | Msa1390870 | -0.812356 | 4.417368e-51 | -8.615850e-47 |
| Msa1351380 | Msa1390870 | -0.820335 | 7.243656e-53 | -8.615850e-47 |
| Msa1356500 | Msa1390870 | -0.809261 | 2.064880e-50 | -8.615850e-47 |
| Msa1366400 | Msa1390870 | -0.801787 | 7.637101e-49 | -8.615850e-47 |
| Msa1368150 | Msa1390870 | -0.813697 | 2.244457e-51 | -8.615850e-47 |
| Msa1372570 | Msa1390870 | -0.809284 | 2.041748e-50 | -8.615850e-47 |
| Msa1376010 | Msa1390870 | -0.811743 | 6.009336e-51 | -8.615850e-47 |
| Msa1376510 | Msa1390870 | -0.804035 | 2.620471e-49 | -8.615850e-47 |
| Msa1379270 | Msa1390870 | -0.822486 | 2.309295e-53 | -8.615850e-47 |
| Msa1390870 | Msa1393550 | -0.804155 | 2.473610e-49 | -8.615850e-47 |
| Msa1390870 | Msa1396310 | -0.820924 | 5.303530e-53 | -8.615850e-47 |
| Msa1390870 | Msa1412810 | -0.801347 | 9.398280e-49 | -8.615850e-47 |
| Msa1390870 | Msa1414000 | -0.823600 | 1.269709e-53 | -8.615850e-47 |
| Msa1390870 | Msa1418010 | -0.824030 | 1.006328e-53 | -8.615850e-47 |
| Msa1390870 | Msa1418840 | -0.805227 | 1.477645e-49 | -8.615850e-47 |
| Msa1390870 | Msa1426310 | -0.805038 | 1.618582e-49 | -8.615850e-47 |
| Msa1390870 | Msa1429180 | -0.800052 | 1.726605e-48 | -8.615850e-47 |
| Msa1390870 | Msa1436660 | -0.811420 | 7.065376e-51 | -8.615850e-47 |
| Msa1390870 | Msa1453540 | -0.808308 | 3.301282e-50 | -8.615850e-47 |
| Msa1390870 | Msa1459590 | -0.803283 | 3.752661e-49 | -8.615850e-47 |
| Msa1390870 | Msa1463500 | -0.836261 | 1.038081e-56 | -8.615850e-47 |
| Msa1390870 | Msa1465380 | -0.820595 | 6.311855e-53 | -8.615850e-47 |
| Msa0612890 | Msa1390870 | -0.809643 | 1.709611e-50 | -8.615850e-47 |
| Msa0626900 | Msa1390870 | -0.805409 | 1.353906e-49 | -8.615850e-47 |
| Msa0638040 | Msa1390870 | -0.814626 | 1.399576e-51 | -8.615850e-47 |
| Msa0653500 | Msa1390870 | -0.805749 | 1.148875e-49 | -8.615850e-47 |
| Msa0654560 | Msa1390870 | -0.810379 | 1.187143e-50 | -8.615850e-47 |
| Msa0660070 | Msa1390870 | -0.812396 | 4.329314e-51 | -8.615850e-47 |
| Msa0662230 | Msa1390870 | -0.819113 | 1.377228e-52 | -8.615850e-47 |
| Msa0667680 | Msa1390870 | -0.812571 | 3.964226e-51 | -8.615850e-47 |
| Msa0677110 | Msa1390870 | -0.815243 | 1.021534e-51 | -8.615850e-47 |
| Msa0701460 | Msa1390870 | -0.806837 | 6.775226e-50 | -8.615850e-47 |
| Msa0701530 | Msa1390870 | -0.808977 | 2.375673e-50 | -8.615850e-47 |
| Msa0706130 | Msa1390870 | -0.803360 | 3.618616e-49 | -8.615850e-47 |
| Msa0707270 | Msa1390870 | -0.817708 | 2.866822e-52 | -8.615850e-47 |
| Msa0708380 | Msa1390870 | -0.817574 | 3.072828e-52 | -8.615850e-47 |
| Msa0716670 | Msa1390870 | -0.809921 | 1.489499e-50 | -8.615850e-47 |
| Msa0720850 | Msa1390870 | -0.805474 | 1.311654e-49 | -8.615850e-47 |
| Msa0729590 | Msa1390870 | -0.807298 | 5.412029e-50 | -8.615850e-47 |
| Msa0751540 | Msa1390870 | -0.807723 | 4.398337e-50 | -8.615850e-47 |
| Msa0752830 | Msa1390870 | -0.815670 | 8.206413e-52 | -8.615850e-47 |
| Msa0766910 | Msa1390870 | -0.810428 | 1.158512e-50 | -8.615850e-47 |
| Msa0772400 | Msa1390870 | -0.805695 | 1.179157e-49 | -8.615850e-47 |
| Msa0786330 | Msa1390870 | -0.811922 | 5.494861e-51 | -8.615850e-47 |
| Msa0803930 | Msa1390870 | -0.813668 | 2.277677e-51 | -8.615850e-47 |
| Msa0821030 | Msa1390870 | -0.805673 | 1.191686e-49 | -8.615850e-47 |
| Msa0822800 | Msa1390870 | -0.817743 | 2.814492e-52 | -8.615850e-47 |
| Msa0840450 | Msa1390870 | -0.804304 | 2.303243e-49 | -8.615850e-47 |
| Msa0143950 | Msa1390870 | 0.814198 | 1.740855e-51 | -8.615850e-47 |
| Msa0160470 | Msa1390870 | -0.831694 | 1.443852e-55 | -8.615850e-47 |
| Msa0163400 | Msa1390870 | -0.808814 | 2.573644e-50 | -8.615850e-47 |
| Msa0164580 | Msa1390870 | -0.803726 | 3.038668e-49 | -8.615850e-47 |
| Msa0169320 | Msa1390870 | -0.814604 | 1.415574e-51 | -8.615850e-47 |
| Msa0170730 | Msa1390870 | -0.820345 | 7.205150e-53 | -8.615850e-47 |
| Msa0213020 | Msa1390870 | -0.811391 | 7.166377e-51 | -8.615850e-47 |
| Msa0213510 | Msa1390870 | -0.810970 | 8.846046e-51 | -8.615850e-47 |
| Msa0213590 | Msa1390870 | -0.812564 | 3.979338e-51 | -8.615850e-47 |
| Msa0237440 | Msa1390870 | -0.821789 | 3.350337e-53 | -8.615850e-47 |
| Msa1225140 | Msa1390870 | -0.810249 | 1.265919e-50 | -8.615850e-47 |
| Msa1251890 | Msa1390870 | -0.803622 | 3.193195e-49 | -8.615850e-47 |
| Msa1270850 | Msa1390870 | -0.802365 | 5.807898e-49 | -8.615850e-47 |
| Msa1279240 | Msa1390870 | -0.800357 | 1.496682e-48 | -8.615850e-47 |
| Msa1288970 | Msa1390870 | -0.800123 | 1.670528e-48 | -8.615850e-47 |
| Msa1291070 | Msa1390870 | -0.803193 | 3.917092e-49 | -8.615850e-47 |
| Msa1296270 | Msa1390870 | -0.826143 | 3.188375e-54 | -8.615850e-47 |
| Msa1301610 | Msa1390870 | -0.802915 | 4.472678e-49 | -8.615850e-47 |
| Msa1317340 | Msa1390870 | -0.813026 | 3.152757e-51 | -8.615850e-47 |
| Msa1330910 | Msa1390870 | -0.822468 | 2.330827e-53 | -8.615850e-47 |
| Msa0367310 | Msa1390870 | -0.807332 | 5.323405e-50 | -8.615850e-47 |
| Msa0372550 | Msa1390870 | -0.811350 | 7.315801e-51 | -8.615850e-47 |
| Msa0375570 | Msa1390870 | -0.812850 | 3.444187e-51 | -8.615850e-47 |
| Msa0376480 | Msa1390870 | -0.835504 | 1.615134e-56 | -8.615850e-47 |
| Msa0379950 | Msa1390870 | -0.829192 | 5.906994e-55 | -8.615850e-47 |
| Msa0404880 | Msa1390870 | -0.820975 | 5.161975e-53 | -8.615850e-47 |
| Msa0424220 | Msa1390870 | -0.823150 | 1.616754e-53 | -8.615850e-47 |
| Msa0430040 | Msa1390870 | -0.814516 | 1.480618e-51 | -8.615850e-47 |
| Msa0443250 | Msa1390870 | -0.802892 | 4.520676e-49 | -8.615850e-47 |
| Msa0450430 | Msa1390870 | -0.802553 | 5.312286e-49 | -8.615850e-47 |
| Msa0463580 | Msa1390870 | -0.800741 | 1.249924e-48 | -8.615850e-47 |
| Msa0471130 | Msa1390870 | -0.818797 | 1.625388e-52 | -8.615850e-47 |
| Msa0475080 | Msa1390870 | -0.801056 | 1.077947e-48 | -8.615850e-47 |
| Msa0476820 | Msa1390870 | -0.812906 | 3.348329e-51 | -8.615850e-47 |
| Msa0481050 | Msa1390870 | -0.825326 | 4.982739e-54 | -8.615850e-47 |
| Msa0985570 | Msa1390870 | -0.826880 | 2.128304e-54 | -8.615850e-47 |
| Msa0986620 | Msa1390870 | -0.803487 | 3.404750e-49 | -8.615850e-47 |
| Msa1031650 | Msa1390870 | -0.804125 | 2.509839e-49 | -8.615850e-47 |
| Msa1044790 | Msa1390870 | -0.819853 | 9.337324e-53 | -8.615850e-47 |
| Msa1057340 | Msa1390870 | -0.808811 | 2.578416e-50 | -8.615850e-47 |
| Msa1066930 | Msa1390870 | -0.838519 | 2.740199e-57 | -8.615850e-47 |
| Msa1078600 | Msa1390870 | -0.800428 | 1.448178e-48 | -8.615850e-47 |
| Msa1078770 | Msa1390870 | -0.801728 | 7.850498e-49 | -8.615850e-47 |
| Msa0266100 | Msa1390870 | -0.805690 | 1.182080e-49 | -8.615850e-47 |
| Msa0285510 | Msa1390870 | -0.826243 | 3.018864e-54 | -8.615850e-47 |
| Msa0292150 | Msa1390870 | -0.812071 | 5.097168e-51 | -8.615850e-47 |
| Msa0304020 | Msa1390870 | -0.805785 | 1.128735e-49 | -8.615850e-47 |
| Msa0312820 | Msa1390870 | 0.945107 | 5.666106e-104 | -8.615850e-47 |
| Msa0330970 | Msa1390870 | -0.806260 | 8.969244e-50 | -8.615850e-47 |
| Msa0339340 | Msa1390870 | -0.801919 | 7.175520e-49 | -8.615850e-47 |
| Msa0341710 | Msa1390870 | -0.807615 | 4.636781e-50 | -8.615850e-47 |
| Msa1109500 | Msa1390870 | -0.819113 | 1.377443e-52 | -8.615850e-47 |
| Msa1126980 | Msa1390870 | -0.815856 | 7.460299e-52 | -8.615850e-47 |
| Msa1143860 | Msa1390870 | -0.814168 | 1.767034e-51 | -8.615850e-47 |
| Msa1144420 | Msa1390870 | -0.813755 | 2.180327e-51 | -8.615850e-47 |
| Msa1149570 | Msa1390870 | -0.819444 | 1.157972e-52 | -8.615850e-47 |
| Msa1154130 | Msa1390870 | -0.803280 | 3.758085e-49 | -8.615850e-47 |
| Msa1154250 | Msa1390870 | -0.811061 | 8.450390e-51 | -8.615850e-47 |
| Msa1166020 | Msa1390870 | -0.821121 | 4.778851e-53 | -8.615850e-47 |
| Msa1197210 | Msa1390870 | -0.809605 | 1.741805e-50 | -8.615850e-47 |
| Msa1199690 | Msa1390870 | -0.816937 | 4.273426e-52 | -8.615850e-47 |
| Msa1203840 | Msa1390870 | -0.811076 | 8.386901e-51 | -8.615850e-47 |
| Msa1203860 | Msa1390870 | -0.818172 | 2.251484e-52 | -8.615850e-47 |
| Msa1215790 | Msa1390870 | -0.800155 | 1.645774e-48 | -8.615850e-47 |
| Msa1224310 | Msa1390870 | -0.824948 | 6.121933e-54 | -8.615850e-47 |
| Msa0485210 | Msa1390870 | -0.800636 | 1.313312e-48 | -8.615850e-47 |
| Msa0500490 | Msa1390870 | -0.808859 | 2.518360e-50 | -8.615850e-47 |
| Msa0513660 | Msa1390870 | -0.810694 | 1.015042e-50 | -8.615850e-47 |
| Msa0516340 | Msa1390870 | -0.830952 | 2.197761e-55 | -8.615850e-47 |
| Msa0522810 | Msa1390870 | -0.805836 | 1.101203e-49 | -8.615850e-47 |
| Msa0530220 | Msa1390870 | -0.810019 | 1.419462e-50 | -8.615850e-47 |
| Msa0530250 | Msa1390870 | -0.825000 | 5.949105e-54 | -8.615850e-47 |
| Msa0546020 | Msa1390870 | -0.817431 | 3.308797e-52 | -8.615850e-47 |
| Msa0576640 | Msa1390870 | -0.813903 | 2.021714e-51 | -8.615850e-47 |
| Msa0581190 | Msa1390870 | -0.819510 | 1.118455e-52 | -8.615850e-47 |
| Msa0899140 | Msa1390870 | -0.815754 | 7.860751e-52 | -8.615850e-47 |
| Msa0910650 | Msa1390870 | -0.802118 | 6.529861e-49 | -8.615850e-47 |
| Msa0932040 | Msa1390870 | -0.810466 | 1.136879e-50 | -8.615850e-47 |
| Msa0967670 | Msa1390870 | -0.807626 | 4.612033e-50 | -8.615850e-47 |
| Msa0970160 | Msa1390870 | -0.812651 | 3.808964e-51 | -8.615850e-47 |
| Msa0974410 | Msa1390870 | -0.819918 | 9.023793e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1390870 | MtrunA17_Chr2g0282361 | 87.891 | 256 | 12 | 5 | 1 | 241 | 1 | 252 | 4.08e-136 | 384 |
| Msa1390870 | MtrunA17_Chr4g0047141 | 68.205 | 195 | 48 | 9 | 65 | 248 | 50 | 241 | 3.16e-64 | 200 |
| Msa1390870 | MtrunA17_Chr2g0317321 | 56.716 | 201 | 59 | 7 | 61 | 242 | 51 | 242 | 1.98e-53 | 173 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1390870 | AT1G28280.2 | 57.438 | 242 | 61 | 11 | 34 | 242 | 7 | 239 | 1.35e-66 | 207 |
| Msa1390870 | AT1G28280.1 | 57.261 | 241 | 61 | 11 | 34 | 241 | 7 | 238 | 4.59e-66 | 206 |
| Msa1390870 | AT3G15300.1 | 50.249 | 201 | 64 | 12 | 65 | 242 | 29 | 216 | 2.00e-38 | 134 |
| Msa1390870 | AT5G53830.1 | 52.970 | 202 | 54 | 12 | 72 | 239 | 45 | 239 | 3.60e-37 | 131 |
| Msa1390870 | AT2G33780.1 | 45.026 | 191 | 78 | 7 | 66 | 245 | 26 | 200 | 7.38e-34 | 121 |
| Msa1390870 | AT1G80450.1 | 36.842 | 171 | 84 | 8 | 76 | 242 | 15 | 165 | 7.11e-14 | 68.2 |
| Msa1390870 | AT5G08480.2 | 35.673 | 171 | 91 | 6 | 76 | 243 | 17 | 171 | 8.23e-11 | 59.7 |
| Msa1390870 | AT5G08480.3 | 35.673 | 171 | 91 | 6 | 76 | 243 | 17 | 171 | 8.23e-11 | 59.7 |
Find 62 sgRNAs with CRISPR-Local
Find 148 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAACAAATGTTGTTGGATA+TGG | 0.219375 | tig0021393:-67248 | None:intergenic |
| GAAGAACCGGATCGGTTAAA+TGG | 0.240342 | tig0021393:-67575 | None:intergenic |
| GAAACTTGAGTTGGAGAAAA+TGG | 0.246762 | tig0021393:-67462 | None:intergenic |
| ACCTGGTTCGGATCTTGTTA+TGG | 0.250404 | tig0021393:-67222 | None:intergenic |
| CCTGGTTCGGATCTTGTTAT+GGG | 0.271576 | tig0021393:-67221 | None:intergenic |
| TGCTAGAAATGGTTCTTCTT+TGG | 0.294813 | tig0021393:+67604 | Msa1390870:CDS |
| CAAGTTGTTCAAATGTTAAC+TGG | 0.300641 | tig0021393:+67293 | Msa1390870:CDS |
| TGGGTCTTGGTGAAGGAATT+TGG | 0.302573 | tig0021393:-67148 | None:intergenic |
| ACTACTTCTCCTAGGGCTTC+AGG | 0.309005 | tig0021393:+67737 | Msa1390870:CDS |
| GAGAAAATGGGTGGTAATAA+AGG | 0.316316 | tig0021393:-67449 | None:intergenic |
| TTGTGTCCATTACTTGTTAT+GGG | 0.319171 | tig0021393:-67167 | None:intergenic |
| GTTGTGTCCATTACTTGTTA+TGG | 0.323237 | tig0021393:-67168 | None:intergenic |
| TCGGGTATGAGTGGTGTAAC+CGG | 0.325510 | tig0021393:-67551 | None:intergenic |
| CCATTACTTGTTATGGGTCT+TGG | 0.332135 | tig0021393:-67161 | None:intergenic |
| TGGGTCTTGGTGATTGAATT+TGG | 0.337028 | tig0021393:-67202 | None:intergenic |
| GTAATAAAGGGTTAATGTTA+AGG | 0.349071 | tig0021393:-67436 | None:intergenic |
| TCGGATCTTGTTATGGGTCT+TGG | 0.376668 | tig0021393:-67215 | None:intergenic |
| TTGGTGGAATGTTATGGTTA+TGG | 0.382884 | tig0021393:-67361 | None:intergenic |
| TCTTCTTCTGCTGCTAGAAA+TGG | 0.396481 | tig0021393:+67593 | Msa1390870:CDS |
| GATAAAGCTATAAGAGAAAA+GGG | 0.402366 | tig0021393:+67641 | Msa1390870:CDS |
| TTGGTGATTGAATTTGGTTA+TGG | 0.403350 | tig0021393:-67196 | None:intergenic |
| GTCTCTAGGAGTTGAAGCTC+TGG | 0.403528 | tig0021393:-67684 | None:intergenic |
| ATTGAATTTGGTTATGGCTA+TGG | 0.404356 | tig0021393:-67190 | None:intergenic |
| TGGATATGGGTTACCTGGTT+CGG | 0.412484 | tig0021393:-67234 | None:intergenic |
| AAGGAATTTGGTTATGGCTA+TGG | 0.434064 | tig0021393:-67136 | None:intergenic |
| TTGGTGAAGGAATTTGGTTA+TGG | 0.445585 | tig0021393:-67142 | None:intergenic |
| AGATAAAGCTATAAGAGAAA+AGG | 0.449288 | tig0021393:+67640 | Msa1390870:CDS |
| GAACAAATGTTGTTGGATAT+GGG | 0.451854 | tig0021393:-67247 | None:intergenic |
| GAGCAAGAGAAGGAAAATCT+AGG | 0.454989 | tig0021393:-67523 | None:intergenic |
| TCCTTCTCTTGCTCTTAGCC+CGG | 0.462751 | tig0021393:+67532 | Msa1390870:CDS |
| GGAATGTTATGGTTATGGTT+TGG | 0.475070 | tig0021393:-67356 | None:intergenic |
| TCAGCTTGAACAAATGTTGT+TGG | 0.479156 | tig0021393:-67254 | None:intergenic |
| CCGGATCGGTTAAATGGGTC+GGG | 0.490143 | tig0021393:-67569 | None:intergenic |
| AGAAAATGGGTGGTAATAAA+GGG | 0.497203 | tig0021393:-67448 | None:intergenic |
| TCTAGCAGCAGAAGAAGAAC+CGG | 0.502830 | tig0021393:-67588 | None:intergenic |
| CCCGACCCATTTAACCGATC+CGG | 0.503223 | tig0021393:+67569 | Msa1390870:CDS |
| AAGAACCGGATCGGTTAAAT+GGG | 0.503269 | tig0021393:-67574 | None:intergenic |
| CCAAGACCCATAACAAGTAA+TGG | 0.503842 | tig0021393:+67161 | Msa1390870:CDS |
| CTTGTTATGGGTCTTGGTGA+AGG | 0.506753 | tig0021393:-67155 | None:intergenic |
| TCAAATGTTAACTGGTTCCT+CGG | 0.513372 | tig0021393:+67301 | Msa1390870:CDS |
| GGAGTTGAAGCTCTGGGAGA+AGG | 0.513871 | tig0021393:-67677 | None:intergenic |
| GAAGGAAAATCTAGGATACT+AGG | 0.515704 | tig0021393:-67515 | None:intergenic |
| CGGGTATGAGTGGTGTAACC+GGG | 0.519254 | tig0021393:-67550 | None:intergenic |
| ACCGGATCGGTTAAATGGGT+CGG | 0.532624 | tig0021393:-67570 | None:intergenic |
| CATTGATGATGTGGAATCCA+TGG | 0.533309 | tig0021393:-67030 | None:intergenic |
| AAACTTGAGTTGGAGAAAAT+GGG | 0.542299 | tig0021393:-67461 | None:intergenic |
| GTTGTTGGATATGGGTTACC+TGG | 0.543727 | tig0021393:-67239 | None:intergenic |
| TTGGTATCTCATTGATGATG+TGG | 0.551120 | tig0021393:-67039 | None:intergenic |
| GAAGCCCTAGGAGAAGTAGT+AGG | 0.571572 | tig0021393:-67734 | None:intergenic |
| CAAACTCAAACTCAAAGCAA+TGG | 0.576732 | tig0021393:+67107 | Msa1390870:CDS |
| GATGAAGGACCTGAAGCCCT+AGG | 0.577284 | tig0021393:-67746 | None:intergenic |
| TCTCTAGGAGTTGAAGCTCT+GGG | 0.580776 | tig0021393:-67683 | None:intergenic |
| AAACGAGGTTCAGAGTCTCT+AGG | 0.581466 | tig0021393:-67698 | None:intergenic |
| ACGAGGTGAGAAACTTGAGT+TGG | 0.589923 | tig0021393:-67471 | None:intergenic |
| CCCATAACAAGATCCGAACC+AGG | 0.590432 | tig0021393:+67221 | Msa1390870:CDS |
| TTAAATGGGTCGGGTATGAG+TGG | 0.616779 | tig0021393:-67560 | None:intergenic |
| AAGATCTCTTGTTTGTTACG+AGG | 0.644449 | tig0021393:-67488 | None:intergenic |
| TTGAACAACTTGTTTGAACG+AGG | 0.648447 | tig0021393:-67282 | None:intergenic |
| CAGCAGAAGAAGAACCGGAT+CGG | 0.652832 | tig0021393:-67583 | None:intergenic |
| ACCGGGCTAAGAGCAAGAGA+AGG | 0.662342 | tig0021393:-67533 | None:intergenic |
| CTTGAGTTGGAGAAAATGGG+TGG | 0.666617 | tig0021393:-67458 | None:intergenic |
| ATCATCAATGAGATACCAAG+AGG | 0.685459 | tig0021393:+67043 | Msa1390870:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGAAACCTTGTTGTTTTTT+TGG | - | tig0021393:67383-67402 | None:intergenic | 25.0% |
| ! | GTAATAAAGGGTTAATGTTA+AGG | - | tig0021393:67439-67458 | None:intergenic | 25.0% |
| ! | AGATAAAGCTATAAGAGAAA+AGG | + | tig0021393:67640-67659 | Msa1390870:CDS | 25.0% |
| ! | GATAAAGCTATAAGAGAAAA+GGG | + | tig0021393:67641-67660 | Msa1390870:CDS | 25.0% |
| !!! | GAATATTTTGAAGAAGATGA+AGG | - | tig0021393:67764-67783 | None:intergenic | 25.0% |
| !!! | TTGAAACCTTGTTGTTTTTT+TGG | - | tig0021393:67383-67402 | None:intergenic | 25.0% |
| ! | GTAATAAAGGGTTAATGTTA+AGG | - | tig0021393:67439-67458 | None:intergenic | 25.0% |
| ! | AGATAAAGCTATAAGAGAAA+AGG | + | tig0021393:67640-67659 | Msa1390870:CDS | 25.0% |
| ! | GATAAAGCTATAAGAGAAAA+GGG | + | tig0021393:67641-67660 | Msa1390870:CDS | 25.0% |
| !!! | GAATATTTTGAAGAAGATGA+AGG | - | tig0021393:67764-67783 | None:intergenic | 25.0% |
| !! | TGAGTTTGAGTTTGAGTTTT+TGG | - | tig0021393:67101-67120 | None:intergenic | 30.0% |
| !! | TTGTGTCCATTACTTGTTAT+GGG | - | tig0021393:67170-67189 | None:intergenic | 30.0% |
| ATTGAATTTGGTTATGGCTA+TGG | - | tig0021393:67193-67212 | None:intergenic | 30.0% | |
| ! | TTGGTGATTGAATTTGGTTA+TGG | - | tig0021393:67199-67218 | None:intergenic | 30.0% |
| GAACAAATGTTGTTGGATAT+GGG | - | tig0021393:67250-67269 | None:intergenic | 30.0% | |
| TGAACAAATGTTGTTGGATA+TGG | - | tig0021393:67251-67270 | None:intergenic | 30.0% | |
| CAAGTTGTTCAAATGTTAAC+TGG | + | tig0021393:67293-67312 | Msa1390870:CDS | 30.0% | |
| !!! | GTTTTTTTGGTGGAATGTTA+TGG | - | tig0021393:67370-67389 | None:intergenic | 30.0% |
| ATTCCACCAAAAAAACAACA+AGG | + | tig0021393:67374-67393 | Msa1390870:CDS | 30.0% | |
| !!! | AAACCTTGTTGTTTTTTTGG+TGG | - | tig0021393:67380-67399 | None:intergenic | 30.0% |
| AGAAAATGGGTGGTAATAAA+GGG | - | tig0021393:67451-67470 | None:intergenic | 30.0% | |
| !! | AAACTTGAGTTGGAGAAAAT+GGG | - | tig0021393:67464-67483 | None:intergenic | 30.0% |
| !! | TGAGTTTGAGTTTGAGTTTT+TGG | - | tig0021393:67101-67120 | None:intergenic | 30.0% |
| !! | TTGTGTCCATTACTTGTTAT+GGG | - | tig0021393:67170-67189 | None:intergenic | 30.0% |
| ATTGAATTTGGTTATGGCTA+TGG | - | tig0021393:67193-67212 | None:intergenic | 30.0% | |
| ! | TTGGTGATTGAATTTGGTTA+TGG | - | tig0021393:67199-67218 | None:intergenic | 30.0% |
| GAACAAATGTTGTTGGATAT+GGG | - | tig0021393:67250-67269 | None:intergenic | 30.0% | |
| TGAACAAATGTTGTTGGATA+TGG | - | tig0021393:67251-67270 | None:intergenic | 30.0% | |
| CAAGTTGTTCAAATGTTAAC+TGG | + | tig0021393:67293-67312 | Msa1390870:CDS | 30.0% | |
| !!! | GTTTTTTTGGTGGAATGTTA+TGG | - | tig0021393:67370-67389 | None:intergenic | 30.0% |
| ATTCCACCAAAAAAACAACA+AGG | + | tig0021393:67374-67393 | Msa1390870:CDS | 30.0% | |
| !!! | AAACCTTGTTGTTTTTTTGG+TGG | - | tig0021393:67380-67399 | None:intergenic | 30.0% |
| AGAAAATGGGTGGTAATAAA+GGG | - | tig0021393:67451-67470 | None:intergenic | 30.0% | |
| !! | AAACTTGAGTTGGAGAAAAT+GGG | - | tig0021393:67464-67483 | None:intergenic | 30.0% |
| !! | TTGGTATCTCATTGATGATG+TGG | - | tig0021393:67042-67061 | None:intergenic | 35.0% |
| ATCATCAATGAGATACCAAG+AGG | + | tig0021393:67043-67062 | Msa1390870:CDS | 35.0% | |
| ! | GAGATGATTTTTGTTCCTCT+TGG | - | tig0021393:67061-67080 | None:intergenic | 35.0% |
| !!! | TGAGTTTGAGTTTTTGGTGA+AGG | - | tig0021393:67095-67114 | None:intergenic | 35.0% |
| CAAACTCAAACTCAAAGCAA+TGG | + | tig0021393:67107-67126 | Msa1390870:CDS | 35.0% | |
| AAGGAATTTGGTTATGGCTA+TGG | - | tig0021393:67139-67158 | None:intergenic | 35.0% | |
| TTGGTGAAGGAATTTGGTTA+TGG | - | tig0021393:67145-67164 | None:intergenic | 35.0% | |
| ! | GTTGTGTCCATTACTTGTTA+TGG | - | tig0021393:67171-67190 | None:intergenic | 35.0% |
| TCAGCTTGAACAAATGTTGT+TGG | - | tig0021393:67257-67276 | None:intergenic | 35.0% | |
| ! | TTGAACAACTTGTTTGAACG+AGG | - | tig0021393:67285-67304 | None:intergenic | 35.0% |
| ! | TCAAATGTTAACTGGTTCCT+CGG | + | tig0021393:67301-67320 | Msa1390870:CDS | 35.0% |
| GGAATGTTATGGTTATGGTT+TGG | - | tig0021393:67359-67378 | None:intergenic | 35.0% | |
| TTGGTGGAATGTTATGGTTA+TGG | - | tig0021393:67364-67383 | None:intergenic | 35.0% | |
| GAGAAAATGGGTGGTAATAA+AGG | - | tig0021393:67452-67471 | None:intergenic | 35.0% | |
| !! | GAAACTTGAGTTGGAGAAAA+TGG | - | tig0021393:67465-67484 | None:intergenic | 35.0% |
| ! | AAGATCTCTTGTTTGTTACG+AGG | - | tig0021393:67491-67510 | None:intergenic | 35.0% |
| ! | GAAGGAAAATCTAGGATACT+AGG | - | tig0021393:67518-67537 | None:intergenic | 35.0% |
| TGCTAGAAATGGTTCTTCTT+TGG | + | tig0021393:67604-67623 | Msa1390870:CDS | 35.0% | |
| ! | TTTTTCCTACTACTTCTCCT+AGG | + | tig0021393:67729-67748 | Msa1390870:CDS | 35.0% |
| ! | TTTTCCTACTACTTCTCCTA+GGG | + | tig0021393:67730-67749 | Msa1390870:CDS | 35.0% |
| !! | TTGGTATCTCATTGATGATG+TGG | - | tig0021393:67042-67061 | None:intergenic | 35.0% |
| ATCATCAATGAGATACCAAG+AGG | + | tig0021393:67043-67062 | Msa1390870:CDS | 35.0% | |
| ! | GAGATGATTTTTGTTCCTCT+TGG | - | tig0021393:67061-67080 | None:intergenic | 35.0% |
| !!! | TGAGTTTGAGTTTTTGGTGA+AGG | - | tig0021393:67095-67114 | None:intergenic | 35.0% |
| CAAACTCAAACTCAAAGCAA+TGG | + | tig0021393:67107-67126 | Msa1390870:CDS | 35.0% | |
| AAGGAATTTGGTTATGGCTA+TGG | - | tig0021393:67139-67158 | None:intergenic | 35.0% | |
| TTGGTGAAGGAATTTGGTTA+TGG | - | tig0021393:67145-67164 | None:intergenic | 35.0% | |
| ! | GTTGTGTCCATTACTTGTTA+TGG | - | tig0021393:67171-67190 | None:intergenic | 35.0% |
| TCAGCTTGAACAAATGTTGT+TGG | - | tig0021393:67257-67276 | None:intergenic | 35.0% | |
| ! | TTGAACAACTTGTTTGAACG+AGG | - | tig0021393:67285-67304 | None:intergenic | 35.0% |
| ! | TCAAATGTTAACTGGTTCCT+CGG | + | tig0021393:67301-67320 | Msa1390870:CDS | 35.0% |
| GGAATGTTATGGTTATGGTT+TGG | - | tig0021393:67359-67378 | None:intergenic | 35.0% | |
| TTGGTGGAATGTTATGGTTA+TGG | - | tig0021393:67364-67383 | None:intergenic | 35.0% | |
| GAGAAAATGGGTGGTAATAA+AGG | - | tig0021393:67452-67471 | None:intergenic | 35.0% | |
| !! | GAAACTTGAGTTGGAGAAAA+TGG | - | tig0021393:67465-67484 | None:intergenic | 35.0% |
| ! | AAGATCTCTTGTTTGTTACG+AGG | - | tig0021393:67491-67510 | None:intergenic | 35.0% |
| ! | GAAGGAAAATCTAGGATACT+AGG | - | tig0021393:67518-67537 | None:intergenic | 35.0% |
| TGCTAGAAATGGTTCTTCTT+TGG | + | tig0021393:67604-67623 | Msa1390870:CDS | 35.0% | |
| ! | TTTTTCCTACTACTTCTCCT+AGG | + | tig0021393:67729-67748 | Msa1390870:CDS | 35.0% |
| ! | TTTTCCTACTACTTCTCCTA+GGG | + | tig0021393:67730-67749 | Msa1390870:CDS | 35.0% |
| !! | CCATTACTTGTTATGGGTCT+TGG | - | tig0021393:67164-67183 | None:intergenic | 40.0% |
| CCAAGACCCATAACAAGTAA+TGG | + | tig0021393:67161-67180 | Msa1390870:CDS | 40.0% | |
| ! | TGGGTCTTGGTGATTGAATT+TGG | - | tig0021393:67205-67224 | None:intergenic | 40.0% |
| GAGCAAGAGAAGGAAAATCT+AGG | - | tig0021393:67526-67545 | None:intergenic | 40.0% | |
| AAGAACCGGATCGGTTAAAT+GGG | - | tig0021393:67577-67596 | None:intergenic | 40.0% | |
| TCTTCTTCTGCTGCTAGAAA+TGG | + | tig0021393:67593-67612 | Msa1390870:CDS | 40.0% | |
| GGAGAAGTAGTAGGAAAAAG+AGG | - | tig0021393:67728-67747 | None:intergenic | 40.0% | |
| !! | CCATTACTTGTTATGGGTCT+TGG | - | tig0021393:67164-67183 | None:intergenic | 40.0% |
| CCAAGACCCATAACAAGTAA+TGG | + | tig0021393:67161-67180 | Msa1390870:CDS | 40.0% | |
| ! | TGGGTCTTGGTGATTGAATT+TGG | - | tig0021393:67205-67224 | None:intergenic | 40.0% |
| GAGCAAGAGAAGGAAAATCT+AGG | - | tig0021393:67526-67545 | None:intergenic | 40.0% | |
| AAGAACCGGATCGGTTAAAT+GGG | - | tig0021393:67577-67596 | None:intergenic | 40.0% | |
| TCTTCTTCTGCTGCTAGAAA+TGG | + | tig0021393:67593-67612 | Msa1390870:CDS | 40.0% | |
| GGAGAAGTAGTAGGAAAAAG+AGG | - | tig0021393:67728-67747 | None:intergenic | 40.0% | |
| TGGGTCTTGGTGAAGGAATT+TGG | - | tig0021393:67151-67170 | None:intergenic | 45.0% | |
| ! | CTTGTTATGGGTCTTGGTGA+AGG | - | tig0021393:67158-67177 | None:intergenic | 45.0% |
| ! | TCGGATCTTGTTATGGGTCT+TGG | - | tig0021393:67218-67237 | None:intergenic | 45.0% |
| !! | CCTGGTTCGGATCTTGTTAT+GGG | - | tig0021393:67224-67243 | None:intergenic | 45.0% |
| !! | ACCTGGTTCGGATCTTGTTA+TGG | - | tig0021393:67225-67244 | None:intergenic | 45.0% |
| ! | TGGATATGGGTTACCTGGTT+CGG | - | tig0021393:67237-67256 | None:intergenic | 45.0% |
| ! | GTTGTTGGATATGGGTTACC+TGG | - | tig0021393:67242-67261 | None:intergenic | 45.0% |
| !! | CTTGAGTTGGAGAAAATGGG+TGG | - | tig0021393:67461-67480 | None:intergenic | 45.0% |
| ACGAGGTGAGAAACTTGAGT+TGG | - | tig0021393:67474-67493 | None:intergenic | 45.0% | |
| TTAAATGGGTCGGGTATGAG+TGG | - | tig0021393:67563-67582 | None:intergenic | 45.0% | |
| GAAGAACCGGATCGGTTAAA+TGG | - | tig0021393:67578-67597 | None:intergenic | 45.0% | |
| TCTAGCAGCAGAAGAAGAAC+CGG | - | tig0021393:67591-67610 | None:intergenic | 45.0% | |
| ! | TCTCTAGGAGTTGAAGCTCT+GGG | - | tig0021393:67686-67705 | None:intergenic | 45.0% |
| AAACGAGGTTCAGAGTCTCT+AGG | - | tig0021393:67701-67720 | None:intergenic | 45.0% | |
| GGAAAAAGAGGAAGCAAACG+AGG | - | tig0021393:67716-67735 | None:intergenic | 45.0% | |
| TGGGTCTTGGTGAAGGAATT+TGG | - | tig0021393:67151-67170 | None:intergenic | 45.0% | |
| ! | CTTGTTATGGGTCTTGGTGA+AGG | - | tig0021393:67158-67177 | None:intergenic | 45.0% |
| ! | TCGGATCTTGTTATGGGTCT+TGG | - | tig0021393:67218-67237 | None:intergenic | 45.0% |
| !! | CCTGGTTCGGATCTTGTTAT+GGG | - | tig0021393:67224-67243 | None:intergenic | 45.0% |
| !! | ACCTGGTTCGGATCTTGTTA+TGG | - | tig0021393:67225-67244 | None:intergenic | 45.0% |
| ! | TGGATATGGGTTACCTGGTT+CGG | - | tig0021393:67237-67256 | None:intergenic | 45.0% |
| ! | GTTGTTGGATATGGGTTACC+TGG | - | tig0021393:67242-67261 | None:intergenic | 45.0% |
| !! | CTTGAGTTGGAGAAAATGGG+TGG | - | tig0021393:67461-67480 | None:intergenic | 45.0% |
| ACGAGGTGAGAAACTTGAGT+TGG | - | tig0021393:67474-67493 | None:intergenic | 45.0% | |
| TTAAATGGGTCGGGTATGAG+TGG | - | tig0021393:67563-67582 | None:intergenic | 45.0% | |
| GAAGAACCGGATCGGTTAAA+TGG | - | tig0021393:67578-67597 | None:intergenic | 45.0% | |
| TCTAGCAGCAGAAGAAGAAC+CGG | - | tig0021393:67591-67610 | None:intergenic | 45.0% | |
| ! | TCTCTAGGAGTTGAAGCTCT+GGG | - | tig0021393:67686-67705 | None:intergenic | 45.0% |
| AAACGAGGTTCAGAGTCTCT+AGG | - | tig0021393:67701-67720 | None:intergenic | 45.0% | |
| GGAAAAAGAGGAAGCAAACG+AGG | - | tig0021393:67716-67735 | None:intergenic | 45.0% | |
| CCCATAACAAGATCCGAACC+AGG | + | tig0021393:67221-67240 | Msa1390870:CDS | 50.0% | |
| !!! | AGCTTGTTTTGCTGTCTCCG+AGG | - | tig0021393:67321-67340 | None:intergenic | 50.0% |
| TCCTTCTCTTGCTCTTAGCC+CGG | + | tig0021393:67532-67551 | Msa1390870:CDS | 50.0% | |
| TCGGGTATGAGTGGTGTAAC+CGG | - | tig0021393:67554-67573 | None:intergenic | 50.0% | |
| ACCGGATCGGTTAAATGGGT+CGG | - | tig0021393:67573-67592 | None:intergenic | 50.0% | |
| CAGCAGAAGAAGAACCGGAT+CGG | - | tig0021393:67586-67605 | None:intergenic | 50.0% | |
| ! | GTCTCTAGGAGTTGAAGCTC+TGG | - | tig0021393:67687-67706 | None:intergenic | 50.0% |
| GAAGCCCTAGGAGAAGTAGT+AGG | - | tig0021393:67737-67756 | None:intergenic | 50.0% | |
| ACTACTTCTCCTAGGGCTTC+AGG | + | tig0021393:67737-67756 | Msa1390870:CDS | 50.0% | |
| CCCATAACAAGATCCGAACC+AGG | + | tig0021393:67221-67240 | Msa1390870:CDS | 50.0% | |
| !!! | AGCTTGTTTTGCTGTCTCCG+AGG | - | tig0021393:67321-67340 | None:intergenic | 50.0% |
| TCCTTCTCTTGCTCTTAGCC+CGG | + | tig0021393:67532-67551 | Msa1390870:CDS | 50.0% | |
| TCGGGTATGAGTGGTGTAAC+CGG | - | tig0021393:67554-67573 | None:intergenic | 50.0% | |
| ACCGGATCGGTTAAATGGGT+CGG | - | tig0021393:67573-67592 | None:intergenic | 50.0% | |
| CAGCAGAAGAAGAACCGGAT+CGG | - | tig0021393:67586-67605 | None:intergenic | 50.0% | |
| ! | GTCTCTAGGAGTTGAAGCTC+TGG | - | tig0021393:67687-67706 | None:intergenic | 50.0% |
| GAAGCCCTAGGAGAAGTAGT+AGG | - | tig0021393:67737-67756 | None:intergenic | 50.0% | |
| ACTACTTCTCCTAGGGCTTC+AGG | + | tig0021393:67737-67756 | Msa1390870:CDS | 50.0% | |
| ACCGGGCTAAGAGCAAGAGA+AGG | - | tig0021393:67536-67555 | None:intergenic | 55.0% | |
| CGGGTATGAGTGGTGTAACC+GGG | - | tig0021393:67553-67572 | None:intergenic | 55.0% | |
| CCGGATCGGTTAAATGGGTC+GGG | - | tig0021393:67572-67591 | None:intergenic | 55.0% | |
| CCCGACCCATTTAACCGATC+CGG | + | tig0021393:67569-67588 | Msa1390870:CDS | 55.0% | |
| !! | GGAGTTGAAGCTCTGGGAGA+AGG | - | tig0021393:67680-67699 | None:intergenic | 55.0% |
| GATGAAGGACCTGAAGCCCT+AGG | - | tig0021393:67749-67768 | None:intergenic | 55.0% | |
| ACCGGGCTAAGAGCAAGAGA+AGG | - | tig0021393:67536-67555 | None:intergenic | 55.0% | |
| CGGGTATGAGTGGTGTAACC+GGG | - | tig0021393:67553-67572 | None:intergenic | 55.0% | |
| CCGGATCGGTTAAATGGGTC+GGG | - | tig0021393:67572-67591 | None:intergenic | 55.0% | |
| CCCGACCCATTTAACCGATC+CGG | + | tig0021393:67569-67588 | Msa1390870:CDS | 55.0% | |
| !! | GGAGTTGAAGCTCTGGGAGA+AGG | - | tig0021393:67680-67699 | None:intergenic | 55.0% |
| GATGAAGGACCTGAAGCCCT+AGG | - | tig0021393:67749-67768 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0021393 | gene | 67032 | 67793 | 67032 | ID=Msa1390870;Name=Msa1390870 |
| tig0021393 | mRNA | 67032 | 67793 | 67032 | ID=Msa1390870-mRNA-1;Parent=Msa1390870;Name=Msa1390870-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|253 |
| tig0021393 | exon | 67032 | 67793 | 67032 | ID=Msa1390870-mRNA-1:exon:12434;Parent=Msa1390870-mRNA-1 |
| tig0021393 | CDS | 67032 | 67793 | 67032 | ID=Msa1390870-mRNA-1:cds;Parent=Msa1390870-mRNA-1 |
| Gene Sequence |
| Protein sequence |
