Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71317.t1 | XP_013464631.1 | 88.8 | 80 | 9 | 0 | 48 | 127 | 104 | 183 | 2.20E-26 | 128.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71317.t1 | A0A072VAN1 | 88.8 | 80 | 9 | 0 | 48 | 127 | 104 | 183 | 1.6e-26 | 128.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene71317 | 0.84258 | 2.37E-58 | -1.69E-46 |
| MS.gene049171 | MS.gene71317 | 0.890697 | 7.79E-74 | -1.69E-46 |
| MS.gene049173 | MS.gene71317 | 0.866907 | 2.02E-65 | -1.69E-46 |
| MS.gene049217 | MS.gene71317 | 0.918982 | 7.95E-87 | -1.69E-46 |
| MS.gene049222 | MS.gene71317 | 0.877194 | 7.64E-69 | -1.69E-46 |
| MS.gene049285 | MS.gene71317 | 0.909789 | 3.86E-82 | -1.69E-46 |
| MS.gene049320 | MS.gene71317 | 0.891242 | 4.75E-74 | -1.69E-46 |
| MS.gene04939 | MS.gene71317 | 0.852394 | 4.75E-61 | -1.69E-46 |
| MS.gene049427 | MS.gene71317 | 0.822801 | 1.95E-53 | -1.69E-46 |
| MS.gene049609 | MS.gene71317 | 0.865654 | 5.05E-65 | -1.69E-46 |
| MS.gene049627 | MS.gene71317 | 0.849662 | 2.80E-60 | -1.69E-46 |
| MS.gene049633 | MS.gene71317 | 0.850156 | 2.04E-60 | -1.69E-46 |
| MS.gene049805 | MS.gene71317 | 0.835473 | 1.64E-56 | -1.69E-46 |
| MS.gene05010 | MS.gene71317 | 0.835815 | 1.35E-56 | -1.69E-46 |
| MS.gene050163 | MS.gene71317 | 0.909434 | 5.72E-82 | -1.69E-46 |
| MS.gene050165 | MS.gene71317 | 0.904011 | 1.92E-79 | -1.69E-46 |
| MS.gene050323 | MS.gene71317 | 0.806325 | 8.69E-50 | -1.69E-46 |
| MS.gene050333 | MS.gene71317 | 0.885606 | 7.04E-72 | -1.69E-46 |
| MS.gene050353 | MS.gene71317 | -0.807842 | 4.15E-50 | -1.69E-46 |
| MS.gene050444 | MS.gene71317 | 0.872903 | 2.22E-67 | -1.69E-46 |
| MS.gene050475 | MS.gene71317 | -0.830159 | 3.44E-55 | -1.69E-46 |
| MS.gene050496 | MS.gene71317 | 0.806961 | 6.38E-50 | -1.69E-46 |
| MS.gene050504 | MS.gene71317 | 0.886718 | 2.68E-72 | -1.69E-46 |
| MS.gene050518 | MS.gene71317 | 0.876114 | 1.81E-68 | -1.69E-46 |
| MS.gene050538 | MS.gene71317 | 0.826221 | 3.06E-54 | -1.69E-46 |
| MS.gene050541 | MS.gene71317 | -0.825005 | 5.93E-54 | -1.69E-46 |
| MS.gene050577 | MS.gene71317 | 0.862434 | 5.08E-64 | -1.69E-46 |
| MS.gene050593 | MS.gene71317 | 0.815783 | 7.75E-52 | -1.69E-46 |
| MS.gene050612 | MS.gene71317 | 0.891282 | 4.58E-74 | -1.69E-46 |
| MS.gene050675 | MS.gene71317 | 0.878747 | 2.19E-69 | -1.69E-46 |
| MS.gene050699 | MS.gene71317 | 0.912114 | 2.82E-83 | -1.69E-46 |
| MS.gene050747 | MS.gene71317 | 0.83577 | 1.38E-56 | -1.69E-46 |
| MS.gene050767 | MS.gene71317 | 0.809853 | 1.54E-50 | -1.69E-46 |
| MS.gene050770 | MS.gene71317 | 0.813319 | 2.72E-51 | -1.69E-46 |
| MS.gene050772 | MS.gene71317 | 0.880376 | 5.78E-70 | -1.69E-46 |
| MS.gene05080 | MS.gene71317 | 0.819366 | 1.21E-52 | -1.69E-46 |
| MS.gene05081 | MS.gene71317 | 0.814347 | 1.61E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene71317 | 0.80971 | 1.65E-50 | -1.69E-46 |
| MS.gene050871 | MS.gene71317 | 0.808707 | 2.71E-50 | -1.69E-46 |
| MS.gene050876 | MS.gene71317 | 0.824676 | 7.10E-54 | -1.69E-46 |
| MS.gene050904 | MS.gene71317 | 0.820377 | 7.09E-53 | -1.69E-46 |
| MS.gene050950 | MS.gene71317 | 0.826829 | 2.19E-54 | -1.69E-46 |
| MS.gene050986 | MS.gene71317 | 0.875688 | 2.53E-68 | -1.69E-46 |
| MS.gene05100 | MS.gene71317 | 0.883837 | 3.20E-71 | -1.69E-46 |
| MS.gene05105 | MS.gene71317 | 0.805494 | 1.30E-49 | -1.69E-46 |
| MS.gene051228 | MS.gene71317 | 0.838757 | 2.38E-57 | -1.69E-46 |
| MS.gene051317 | MS.gene71317 | 0.857494 | 1.57E-62 | -1.69E-46 |
| MS.gene051434 | MS.gene71317 | 0.825246 | 5.20E-54 | -1.69E-46 |
| MS.gene051551 | MS.gene71317 | 0.86374 | 2.01E-64 | -1.69E-46 |
| MS.gene051666 | MS.gene71317 | 0.843055 | 1.77E-58 | -1.69E-46 |
| MS.gene051694 | MS.gene71317 | 0.836267 | 1.03E-56 | -1.69E-46 |
| MS.gene051810 | MS.gene71317 | 0.896693 | 2.89E-76 | -1.69E-46 |
| MS.gene051838 | MS.gene71317 | 0.807401 | 5.15E-50 | -1.69E-46 |
| MS.gene051858 | MS.gene71317 | 0.89004 | 1.41E-73 | -1.69E-46 |
| MS.gene051949 | MS.gene71317 | 0.868041 | 8.76E-66 | -1.69E-46 |
| MS.gene052164 | MS.gene71317 | 0.828221 | 1.01E-54 | -1.69E-46 |
| MS.gene052271 | MS.gene71317 | 0.883167 | 5.64E-71 | -1.69E-46 |
| MS.gene052379 | MS.gene71317 | 0.874837 | 4.94E-68 | -1.69E-46 |
| MS.gene052421 | MS.gene71317 | 0.80213 | 6.49E-49 | -1.69E-46 |
| MS.gene052482 | MS.gene71317 | 0.814741 | 1.32E-51 | -1.69E-46 |
| MS.gene052484 | MS.gene71317 | 0.819362 | 1.21E-52 | -1.69E-46 |
| MS.gene052486 | MS.gene71317 | 0.835275 | 1.84E-56 | -1.69E-46 |
| MS.gene052501 | MS.gene71317 | 0.803875 | 2.83E-49 | -1.69E-46 |
| MS.gene052503 | MS.gene71317 | 0.848469 | 6.01E-60 | -1.69E-46 |
| MS.gene052615 | MS.gene71317 | 0.873729 | 1.17E-67 | -1.69E-46 |
| MS.gene052670 | MS.gene71317 | 0.814521 | 1.48E-51 | -1.69E-46 |
| MS.gene052822 | MS.gene71317 | 0.807049 | 6.11E-50 | -1.69E-46 |
| MS.gene052823 | MS.gene71317 | 0.838232 | 3.25E-57 | -1.69E-46 |
| MS.gene052825 | MS.gene71317 | 0.834367 | 3.12E-56 | -1.69E-46 |
| MS.gene052870 | MS.gene71317 | 0.839446 | 1.58E-57 | -1.69E-46 |
| MS.gene052872 | MS.gene71317 | 0.851194 | 1.04E-60 | -1.69E-46 |
| MS.gene052916 | MS.gene71317 | 0.805219 | 1.48E-49 | -1.69E-46 |
| MS.gene052966 | MS.gene71317 | 0.816069 | 6.69E-52 | -1.69E-46 |
| MS.gene053009 | MS.gene71317 | 0.803039 | 4.22E-49 | -1.69E-46 |
| MS.gene053104 | MS.gene71317 | 0.827668 | 1.38E-54 | -1.69E-46 |
| MS.gene053203 | MS.gene71317 | 0.830803 | 2.39E-55 | -1.69E-46 |
| MS.gene053207 | MS.gene71317 | 0.862377 | 5.29E-64 | -1.69E-46 |
| MS.gene053213 | MS.gene71317 | 0.887047 | 2.01E-72 | -1.69E-46 |
| MS.gene053286 | MS.gene71317 | 0.883801 | 3.30E-71 | -1.69E-46 |
| MS.gene053322 | MS.gene71317 | 0.852254 | 5.21E-61 | -1.69E-46 |
| MS.gene053436 | MS.gene71317 | 0.801642 | 8.18E-49 | -1.69E-46 |
| MS.gene053479 | MS.gene71317 | 0.805824 | 1.11E-49 | -1.69E-46 |
| MS.gene053480 | MS.gene71317 | 0.852349 | 4.89E-61 | -1.69E-46 |
| MS.gene053574 | MS.gene71317 | 0.831113 | 2.01E-55 | -1.69E-46 |
| MS.gene053632 | MS.gene71317 | 0.899922 | 1.23E-77 | -1.69E-46 |
| MS.gene053824 | MS.gene71317 | 0.847263 | 1.29E-59 | -1.69E-46 |
| MS.gene053915 | MS.gene71317 | 0.824646 | 7.21E-54 | -1.69E-46 |
| MS.gene053916 | MS.gene71317 | 0.826937 | 2.06E-54 | -1.69E-46 |
| MS.gene054408 | MS.gene71317 | 0.805717 | 1.17E-49 | -1.69E-46 |
| MS.gene054595 | MS.gene71317 | 0.82751 | 1.50E-54 | -1.69E-46 |
| MS.gene054600 | MS.gene71317 | 0.891753 | 2.98E-74 | -1.69E-46 |
| MS.gene054642 | MS.gene71317 | 0.895401 | 9.92E-76 | -1.69E-46 |
| MS.gene05470 | MS.gene71317 | 0.848098 | 7.61E-60 | -1.69E-46 |
| MS.gene054802 | MS.gene71317 | 0.810616 | 1.05E-50 | -1.69E-46 |
| MS.gene054803 | MS.gene71317 | 0.807639 | 4.58E-50 | -1.69E-46 |
| MS.gene054804 | MS.gene71317 | 0.812466 | 4.18E-51 | -1.69E-46 |
| MS.gene054927 | MS.gene71317 | 0.824239 | 8.99E-54 | -1.69E-46 |
| MS.gene054954 | MS.gene71317 | 0.885376 | 8.58E-72 | -1.69E-46 |
| MS.gene05505 | MS.gene71317 | 0.834595 | 2.74E-56 | -1.69E-46 |
| MS.gene055154 | MS.gene71317 | -0.813211 | 2.87E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene71317 | 0.899632 | 1.63E-77 | -1.69E-46 |
| MS.gene055212 | MS.gene71317 | 0.824449 | 8.02E-54 | -1.69E-46 |
| MS.gene055289 | MS.gene71317 | 0.815331 | 9.77E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene71317 | 0.903925 | 2.09E-79 | -1.69E-46 |
| MS.gene055397 | MS.gene71317 | 0.849263 | 3.62E-60 | -1.69E-46 |
| MS.gene055499 | MS.gene71317 | 0.847255 | 1.30E-59 | -1.69E-46 |
| MS.gene055502 | MS.gene71317 | 0.892479 | 1.53E-74 | -1.69E-46 |
| MS.gene055577 | MS.gene71317 | 0.869627 | 2.69E-66 | -1.69E-46 |
| MS.gene055634 | MS.gene71317 | 0.805834 | 1.10E-49 | -1.69E-46 |
| MS.gene055636 | MS.gene71317 | 0.83894 | 2.13E-57 | -1.69E-46 |
| MS.gene055638 | MS.gene71317 | 0.855328 | 6.79E-62 | -1.69E-46 |
| MS.gene055647 | MS.gene71317 | 0.851915 | 6.50E-61 | -1.69E-46 |
| MS.gene055722 | MS.gene71317 | 0.856131 | 3.96E-62 | -1.69E-46 |
| MS.gene055748 | MS.gene71317 | 0.840238 | 9.81E-58 | -1.69E-46 |
| MS.gene055750 | MS.gene71317 | 0.819604 | 1.06E-52 | -1.69E-46 |
| MS.gene055751 | MS.gene71317 | 0.837858 | 4.06E-57 | -1.69E-46 |
| MS.gene055776 | MS.gene71317 | 0.808856 | 2.52E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene71317 | 0.823222 | 1.56E-53 | -1.69E-46 |
| MS.gene05592 | MS.gene71317 | 0.825869 | 3.71E-54 | -1.69E-46 |
| MS.gene055941 | MS.gene71317 | 0.858823 | 6.32E-63 | -1.69E-46 |
| MS.gene055956 | MS.gene71317 | -0.827072 | 1.91E-54 | -1.69E-46 |
| MS.gene056083 | MS.gene71317 | 0.837307 | 5.62E-57 | -1.69E-46 |
| MS.gene056374 | MS.gene71317 | 0.864231 | 1.41E-64 | -1.69E-46 |
| MS.gene056409 | MS.gene71317 | 0.83868 | 2.49E-57 | -1.69E-46 |
| MS.gene056449 | MS.gene71317 | 0.839406 | 1.62E-57 | -1.69E-46 |
| MS.gene056487 | MS.gene71317 | 0.804577 | 2.02E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene71317 | 0.853272 | 2.67E-61 | -1.69E-46 |
| MS.gene056510 | MS.gene71317 | 0.851997 | 6.16E-61 | -1.69E-46 |
| MS.gene056549 | MS.gene71317 | 0.844381 | 7.83E-59 | -1.69E-46 |
| MS.gene056550 | MS.gene71317 | -0.813788 | 2.14E-51 | -1.69E-46 |
| MS.gene056610 | MS.gene71317 | 0.832379 | 9.78E-56 | -1.69E-46 |
| MS.gene056657 | MS.gene71317 | 0.902902 | 6.03E-79 | -1.69E-46 |
| MS.gene056692 | MS.gene71317 | 0.863361 | 2.63E-64 | -1.69E-46 |
| MS.gene056898 | MS.gene71317 | 0.815274 | 1.01E-51 | -1.69E-46 |
| MS.gene056903 | MS.gene71317 | 0.82763 | 1.41E-54 | -1.69E-46 |
| MS.gene056946 | MS.gene71317 | 0.848797 | 4.88E-60 | -1.69E-46 |
| MS.gene057011 | MS.gene71317 | 0.803423 | 3.51E-49 | -1.69E-46 |
| MS.gene057048 | MS.gene71317 | 0.809559 | 1.78E-50 | -1.69E-46 |
| MS.gene057076 | MS.gene71317 | 0.833927 | 4.02E-56 | -1.69E-46 |
| MS.gene057129 | MS.gene71317 | 0.864704 | 1.00E-64 | -1.69E-46 |
| MS.gene057146 | MS.gene71317 | 0.814277 | 1.67E-51 | -1.69E-46 |
| MS.gene057196 | MS.gene71317 | 0.839838 | 1.25E-57 | -1.69E-46 |
| MS.gene057331 | MS.gene71317 | 0.825272 | 5.13E-54 | -1.69E-46 |
| MS.gene057332 | MS.gene71317 | 0.828078 | 1.10E-54 | -1.69E-46 |
| MS.gene057416 | MS.gene71317 | 0.867587 | 1.23E-65 | -1.69E-46 |
| MS.gene057658 | MS.gene71317 | 0.871491 | 6.56E-67 | -1.69E-46 |
| MS.gene057831 | MS.gene71317 | 0.876814 | 1.03E-68 | -1.69E-46 |
| MS.gene057857 | MS.gene71317 | 0.804585 | 2.01E-49 | -1.69E-46 |
| MS.gene057926 | MS.gene71317 | 0.822089 | 2.85E-53 | -1.69E-46 |
| MS.gene058019 | MS.gene71317 | 0.863024 | 3.34E-64 | -1.69E-46 |
| MS.gene058323 | MS.gene71317 | 0.803892 | 2.81E-49 | -1.69E-46 |
| MS.gene058338 | MS.gene71317 | 0.837602 | 4.72E-57 | -1.69E-46 |
| MS.gene05837 | MS.gene71317 | 0.816204 | 6.24E-52 | -1.69E-46 |
| MS.gene058419 | MS.gene71317 | 0.919808 | 2.83E-87 | -1.69E-46 |
| MS.gene058544 | MS.gene71317 | 0.870137 | 1.83E-66 | -1.69E-46 |
| MS.gene058719 | MS.gene71317 | 0.804343 | 2.26E-49 | -1.69E-46 |
| MS.gene058723 | MS.gene71317 | 0.874884 | 4.76E-68 | -1.69E-46 |
| MS.gene058773 | MS.gene71317 | 0.869928 | 2.14E-66 | -1.69E-46 |
| MS.gene058838 | MS.gene71317 | 0.853893 | 1.77E-61 | -1.69E-46 |
| MS.gene05883 | MS.gene71317 | 0.818288 | 2.12E-52 | -1.69E-46 |
| MS.gene059022 | MS.gene71317 | 0.89162 | 3.36E-74 | -1.69E-46 |
| MS.gene059023 | MS.gene71317 | 0.884268 | 2.22E-71 | -1.69E-46 |
| MS.gene059029 | MS.gene71317 | 0.885203 | 9.96E-72 | -1.69E-46 |
| MS.gene059079 | MS.gene71317 | 0.808206 | 3.47E-50 | -1.69E-46 |
| MS.gene05912 | MS.gene71317 | 0.842341 | 2.74E-58 | -1.69E-46 |
| MS.gene05913 | MS.gene71317 | 0.832876 | 7.36E-56 | -1.69E-46 |
| MS.gene05914 | MS.gene71317 | 0.834445 | 2.99E-56 | -1.69E-46 |
| MS.gene05934 | MS.gene71317 | 0.878329 | 3.07E-69 | -1.69E-46 |
| MS.gene059536 | MS.gene71317 | 0.82087 | 5.46E-53 | -1.69E-46 |
| MS.gene059677 | MS.gene71317 | 0.924987 | 3.37E-90 | -1.69E-46 |
| MS.gene059718 | MS.gene71317 | 0.898909 | 3.34E-77 | -1.69E-46 |
| MS.gene059756 | MS.gene71317 | 0.910115 | 2.69E-82 | -1.69E-46 |
| MS.gene059801 | MS.gene71317 | 0.832704 | 8.12E-56 | -1.69E-46 |
| MS.gene059858 | MS.gene71317 | 0.847389 | 1.19E-59 | -1.69E-46 |
| MS.gene059865 | MS.gene71317 | 0.833294 | 5.79E-56 | -1.69E-46 |
| MS.gene059902 | MS.gene71317 | 0.882253 | 1.22E-70 | -1.69E-46 |
| MS.gene059960 | MS.gene71317 | 0.857553 | 1.51E-62 | -1.69E-46 |
| MS.gene060193 | MS.gene71317 | 0.82779 | 1.29E-54 | -1.69E-46 |
| MS.gene060341 | MS.gene71317 | 0.833284 | 5.83E-56 | -1.69E-46 |
| MS.gene060703 | MS.gene71317 | 0.874851 | 4.89E-68 | -1.69E-46 |
| MS.gene060727 | MS.gene71317 | 0.813575 | 2.39E-51 | -1.69E-46 |
| MS.gene060728 | MS.gene71317 | 0.825679 | 4.11E-54 | -1.69E-46 |
| MS.gene060762 | MS.gene71317 | 0.872702 | 2.60E-67 | -1.69E-46 |
| MS.gene06077 | MS.gene71317 | 0.867389 | 1.42E-65 | -1.69E-46 |
| MS.gene060804 | MS.gene71317 | 0.826276 | 2.96E-54 | -1.69E-46 |
| MS.gene060818 | MS.gene71317 | 0.806861 | 6.70E-50 | -1.69E-46 |
| MS.gene060835 | MS.gene71317 | 0.81622 | 6.19E-52 | -1.69E-46 |
| MS.gene06092 | MS.gene71317 | 0.854339 | 1.31E-61 | -1.69E-46 |
| MS.gene060982 | MS.gene71317 | 0.833751 | 4.45E-56 | -1.69E-46 |
| MS.gene061041 | MS.gene71317 | 0.809684 | 1.68E-50 | -1.69E-46 |
| MS.gene061134 | MS.gene71317 | 0.802812 | 4.70E-49 | -1.69E-46 |
| MS.gene061150 | MS.gene71317 | 0.88792 | 9.33E-73 | -1.69E-46 |
| MS.gene061200 | MS.gene71317 | 0.861844 | 7.71E-64 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71317.t1 | MTR_2g078540 | 78.723 | 94 | 19 | 1 | 48 | 141 | 104 | 196 | 7.57e-42 | 140 |
| MS.gene71317.t1 | MTR_6g042030 | 49.412 | 85 | 39 | 2 | 57 | 141 | 112 | 192 | 8.70e-18 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71317.t1 | AT3G15110 | 54.651 | 86 | 37 | 2 | 57 | 141 | 119 | 203 | 1.73e-22 | 89.7 |
Find 45 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACCTGTTCAAGCATTTCTC+GGG | 0.359534 | 2.1:+21988754 | MS.gene71317:CDS |
| TCACCTGTTCAAGCATTTCT+CGG | 0.367357 | 2.1:+21988753 | MS.gene71317:CDS |
| AGAGAAGGAGTAGAATCATA+TGG | 0.370898 | 2.1:-21987489 | None:intergenic |
| TCTTCCGCTCCTTCCGCCGC+AGG | 0.403419 | 2.1:+21987589 | MS.gene71317:CDS |
| GGAGAACACTAGCAGCGATT+TGG | 0.415238 | 2.1:-21987542 | None:intergenic |
| GTCAGTAAAGGAAGAAGCAT+TGG | 0.416654 | 2.1:+21988677 | MS.gene71317:CDS |
| AATCATCTGAAATTGTTTGC+CGG | 0.417137 | 2.1:-21988845 | None:intergenic |
| TGCGGCGGAAGGAGCGGAAG+AGG | 0.447068 | 2.1:-21987587 | None:intergenic |
| GCCCCGAGAAATGCTTGAAC+AGG | 0.450986 | 2.1:-21988756 | None:intergenic |
| ATTGGAGACTTTGAAAGCTA+TGG | 0.451641 | 2.1:+21988695 | MS.gene71317:CDS |
| GCTCAGACCCGCCTCGATGA+TGG | 0.451902 | 2.1:-21988823 | None:intergenic |
| TTGGAGACTTTGAAAGCTAT+GGG | 0.454281 | 2.1:+21988696 | MS.gene71317:CDS |
| TCATCGAGGCGGGTCTGAGC+CGG | 0.484689 | 2.1:+21988826 | MS.gene71317:CDS |
| GAGAAGGAGTAGAATCATAT+GGG | 0.494622 | 2.1:-21987488 | None:intergenic |
| GAAGGAGCGGAAGAGGAGGA+CGG | 0.500736 | 2.1:-21987580 | None:intergenic |
| GAATCATATGGGATTTCAAT+GGG | 0.507790 | 2.1:-21987477 | None:intergenic |
| GCAGCGATTTGGATGAAAGA+AGG | 0.516847 | 2.1:-21987531 | None:intergenic |
| AGAATCATATGGGATTTCAA+TGG | 0.519315 | 2.1:-21987478 | None:intergenic |
| GGCTAAACGTCTCAAACAAA+CGG | 0.521583 | 2.1:+21987611 | MS.gene71317:CDS |
| ATGTTAGTTTGAATCAGATA+AGG | 0.522769 | 2.1:+21988931 | MS.gene71317:CDS |
| CTTCCGCTCCTTCCGCCGCA+GGG | 0.525679 | 2.1:+21987590 | MS.gene71317:CDS |
| TTCGCCACCATCATCGAGGC+GGG | 0.539823 | 2.1:+21988816 | MS.gene71317:CDS |
| GGCGGAAGGAGCGGAAGAGG+AGG | 0.540765 | 2.1:-21987584 | None:intergenic |
| TTCAGGAGAGAAGTCAGTAA+AGG | 0.553365 | 2.1:+21988665 | MS.gene71317:CDS |
| ACGTTTAGCCCTGCGGCGGA+AGG | 0.554548 | 2.1:-21987598 | None:intergenic |
| TAGCCCTGCGGCGGAAGGAG+CGG | 0.556744 | 2.1:-21987593 | None:intergenic |
| ATTACAACAGATGGAGGAGA+TGG | 0.564712 | 2.1:+21987453 | None:intergenic |
| TTTCTCGGGGCAATATCAGC+TGG | 0.567839 | 2.1:+21988768 | MS.gene71317:CDS |
| TCAGTGGAGGAGAGAAGAGA+AGG | 0.568555 | 2.1:-21987504 | None:intergenic |
| GCTGCTAGTGTTCTCCTCAC+CGG | 0.569698 | 2.1:+21987549 | MS.gene71317:CDS |
| CAGCGATTTGGATGAAAGAA+GGG | 0.577029 | 2.1:-21987530 | None:intergenic |
| AGAAATGCTTGAACAGGTGA+AGG | 0.583226 | 2.1:-21988750 | None:intergenic |
| GCTTCAATAGAGACTACTAA+AGG | 0.596943 | 2.1:+21988723 | MS.gene71317:CDS |
| AATGCTTGAACAGGTGAAGG+TGG | 0.599999 | 2.1:-21988747 | None:intergenic |
| CAGACCCGCCTCGATGATGG+TGG | 0.620049 | 2.1:-21988820 | None:intergenic |
| GAGGAGGACGGAAATAGCAC+CGG | 0.620911 | 2.1:-21987568 | None:intergenic |
| ACCTGTTCAAGCATTTCTCG+GGG | 0.627536 | 2.1:+21988755 | MS.gene71317:CDS |
| GAAAGAAGGGTCATCAGTGG+AGG | 0.631336 | 2.1:-21987517 | None:intergenic |
| GATGAAAGAAGGGTCATCAG+TGG | 0.636441 | 2.1:-21987520 | None:intergenic |
| GTTTGAGACGTTTAGCCCTG+CGG | 0.638809 | 2.1:-21987605 | None:intergenic |
| TGAGACGTTTAGCCCTGCGG+CGG | 0.674454 | 2.1:-21987602 | None:intergenic |
| GTTCGCCACCATCATCGAGG+CGG | 0.686352 | 2.1:+21988815 | MS.gene71317:CDS |
| GGACGGAAATAGCACCGGTG+AGG | 0.690490 | 2.1:-21987563 | None:intergenic |
| TAAGTTCGCCACCATCATCG+AGG | 0.692160 | 2.1:+21988812 | MS.gene71317:CDS |
| TGGAGACTTTGAAAGCTATG+GGG | 0.694173 | 2.1:+21988697 | MS.gene71317:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGAGAGAATTAAAATTT+AGG | - | chr2.1:21987986-21988005 | None:intergenic | 15.0% |
| !! | CTTCAATAATTACATATTAA+TGG | - | chr2.1:21988022-21988041 | None:intergenic | 15.0% |
| !!! | ATGATCACTCTTTTTAATAT+AGG | + | chr2.1:21988295-21988314 | MS.gene71317:intron | 20.0% |
| !!! | TGATCACTCTTTTTAATATA+GGG | + | chr2.1:21988296-21988315 | MS.gene71317:intron | 20.0% |
| !!! | TTTAGTCATTTTAAAAACCT+TGG | - | chr2.1:21987807-21987826 | None:intergenic | 20.0% |
| ! | AAAGCTAGTTCAAAAAATGA+GGG | - | chr2.1:21988111-21988130 | None:intergenic | 25.0% |
| ! | CTATAGTTCAAAGACAAAAT+AGG | + | chr2.1:21988241-21988260 | MS.gene71317:intron | 25.0% |
| ! | GATAATCTCATTCTCAATTA+AGG | + | chr2.1:21987723-21987742 | MS.gene71317:intron | 25.0% |
| ! | TTATACTACATCATACAACT+CGG | - | chr2.1:21988075-21988094 | None:intergenic | 25.0% |
| !! | ATGTTAGTTTGAATCAGATA+AGG | + | chr2.1:21988931-21988950 | MS.gene71317:CDS | 25.0% |
| !! | TTGTTTGTGTTGAAGTTTTA+GGG | + | chr2.1:21988564-21988583 | MS.gene71317:intron | 25.0% |
| !! | TTTGTTTGTGTTGAAGTTTT+AGG | + | chr2.1:21988563-21988582 | MS.gene71317:intron | 25.0% |
| !!! | AAATGTGTAAAGCTTTGTTT+TGG | + | chr2.1:21987959-21987978 | MS.gene71317:intron | 25.0% |
| !!! | ATTTTTTGAACTAGCTTTCA+GGG | + | chr2.1:21988113-21988132 | MS.gene71317:intron | 25.0% |
| !!! | CATTGAAATGAGTTTTTTTG+TGG | + | chr2.1:21988625-21988644 | MS.gene71317:intron | 25.0% |
| AAAAGAGTGATCATTCGATT+TGG | - | chr2.1:21988289-21988308 | None:intergenic | 30.0% | |
| AAGAATATGAGTTCAATTCC+TGG | + | chr2.1:21987887-21987906 | MS.gene71317:intron | 30.0% | |
| ACAATTCTTGACCAAAGAAA+TGG | + | chr2.1:21987915-21987934 | MS.gene71317:intron | 30.0% | |
| AGAATATGAGTTCAATTCCT+GGG | + | chr2.1:21987888-21987907 | MS.gene71317:intron | 30.0% | |
| AGAATCATATGGGATTTCAA+TGG | - | chr2.1:21987481-21987500 | None:intergenic | 30.0% | |
| ATTCATGTTATGTTACACCA+AGG | + | chr2.1:21987787-21987806 | MS.gene71317:intron | 30.0% | |
| CAAACAAAAAAGAACAGATG+CGG | - | chr2.1:21988905-21988924 | None:intergenic | 30.0% | |
| GAAAGCTAGTTCAAAAAATG+AGG | - | chr2.1:21988112-21988131 | None:intergenic | 30.0% | |
| GAATCATATGGGATTTCAAT+GGG | - | chr2.1:21987480-21987499 | None:intergenic | 30.0% | |
| TATACTACATCATACAACTC+GGG | - | chr2.1:21988074-21988093 | None:intergenic | 30.0% | |
| TCCTCATCATAAGAAATGTA+AGG | + | chr2.1:21988482-21988501 | MS.gene71317:intron | 30.0% | |
| TTGTGATCCTTGATATATCA+AGG | - | chr2.1:21988401-21988420 | None:intergenic | 30.0% | |
| TTGTTAACCTTCCATTTCTT+TGG | - | chr2.1:21987929-21987948 | None:intergenic | 30.0% | |
| ! | AATCATCTGAAATTGTTTGC+CGG | - | chr2.1:21988848-21988867 | None:intergenic | 30.0% |
| ! | TTTTAATATAGGGCTGCATA+AGG | + | chr2.1:21988306-21988325 | MS.gene71317:intron | 30.0% |
| !! | CCTATTTTAGAAGTAACTGA+GGG | + | chr2.1:21988165-21988184 | MS.gene71317:intron | 30.0% |
| !! | TCCTATTTTAGAAGTAACTG+AGG | + | chr2.1:21988164-21988183 | MS.gene71317:intron | 30.0% |
| !!! | CATTTTTTGAACTAGCTTTC+AGG | + | chr2.1:21988112-21988131 | MS.gene71317:intron | 30.0% |
| !!! | TTTTGTGGTTTTAGCAATTC+AGG | + | chr2.1:21988640-21988659 | MS.gene71317:intron | 30.0% |
| AAAGAACAGATGCGGATAAA+AGG | - | chr2.1:21988897-21988916 | None:intergenic | 35.0% | |
| AACTAGAGAAGTTCATCCTA+AGG | - | chr2.1:21988461-21988480 | None:intergenic | 35.0% | |
| AGAGAAGGAGTAGAATCATA+TGG | - | chr2.1:21987492-21987511 | None:intergenic | 35.0% | |
| CTTGGATTTCAATTTGCCTT+AGG | + | chr2.1:21988442-21988461 | MS.gene71317:intron | 35.0% | |
| GAGAAGGAGTAGAATCATAT+GGG | - | chr2.1:21987491-21987510 | None:intergenic | 35.0% | |
| GATGCAATGAAAACAGTTTG+AGG | + | chr2.1:21988196-21988215 | MS.gene71317:intron | 35.0% | |
| GCAGTTTCCTTGATATATCA+AGG | + | chr2.1:21988391-21988410 | MS.gene71317:intron | 35.0% | |
| GCCTTACATTTCTTATGATG+AGG | - | chr2.1:21988486-21988505 | None:intergenic | 35.0% | |
| GCTTCAATAGAGACTACTAA+AGG | + | chr2.1:21988723-21988742 | MS.gene71317:CDS | 35.0% | |
| TTCATGCTGTTCACATACTA+GGG | + | chr2.1:21987697-21987716 | MS.gene71317:intron | 35.0% | |
| TTCTTGACCAAAGAAATGGA+AGG | + | chr2.1:21987919-21987938 | MS.gene71317:intron | 35.0% | |
| TTTCATGCTGTTCACATACT+AGG | + | chr2.1:21987696-21987715 | MS.gene71317:intron | 35.0% | |
| ! | ATTGGAGACTTTGAAAGCTA+TGG | + | chr2.1:21988695-21988714 | MS.gene71317:CDS | 35.0% |
| ! | TTGGAGACTTTGAAAGCTAT+GGG | + | chr2.1:21988696-21988715 | MS.gene71317:CDS | 35.0% |
| ! | TTTTAGCAATTCAGGTCTTC+AGG | + | chr2.1:21988648-21988667 | MS.gene71317:intron | 35.0% |
| !! | CCCTCAGTTACTTCTAAAAT+AGG | - | chr2.1:21988168-21988187 | None:intergenic | 35.0% |
| AAAACGTTGCTGCTACATCT+TGG | + | chr2.1:21988424-21988443 | MS.gene71317:intron | 40.0% | |
| AGAAATGCTTGAACAGGTGA+AGG | - | chr2.1:21988753-21988772 | None:intergenic | 40.0% | |
| ATATGAGTTCAATTCCTGGG+TGG | + | chr2.1:21987891-21987910 | MS.gene71317:intron | 40.0% | |
| CAGTCTAATACTGCTGATGT+GGG | - | chr2.1:21988372-21988391 | None:intergenic | 40.0% | |
| GCTAGTTCAAAAAATGAGGG+TGG | - | chr2.1:21988108-21988127 | None:intergenic | 40.0% | |
| GGAGAGTGAATTGTAGCTTA+AGG | + | chr2.1:21988262-21988281 | MS.gene71317:intron | 40.0% | |
| GGCTAAACGTCTCAAACAAA+CGG | + | chr2.1:21987611-21987630 | MS.gene71317:CDS | 40.0% | |
| TCACCTGTTCAAGCATTTCT+CGG | + | chr2.1:21988753-21988772 | MS.gene71317:CDS | 40.0% | |
| TTCAGGAGAGAAGTCAGTAA+AGG | + | chr2.1:21988665-21988684 | MS.gene71317:CDS | 40.0% | |
| ! | CAGCGATTTGGATGAAAGAA+GGG | - | chr2.1:21987533-21987552 | None:intergenic | 40.0% |
| ! | GTCAGTAAAGGAAGAAGCAT+TGG | + | chr2.1:21988677-21988696 | MS.gene71317:CDS | 40.0% |
| ! | TGGAGACTTTGAAAGCTATG+GGG | + | chr2.1:21988697-21988716 | MS.gene71317:CDS | 40.0% |
| AATGCTTGAACAGGTGAAGG+TGG | - | chr2.1:21988750-21988769 | None:intergenic | 45.0% | |
| ACCAAGCAATTGAGCTCAAG+TGG | + | chr2.1:21987831-21987850 | MS.gene71317:intron | 45.0% | |
| ACCACTTGAGCTCAATTGCT+TGG | - | chr2.1:21987835-21987854 | None:intergenic | 45.0% | |
| ACCTGTTCAAGCATTTCTCG+GGG | + | chr2.1:21988755-21988774 | MS.gene71317:CDS | 45.0% | |
| ATGCTGTTCACATACTAGGG+AGG | + | chr2.1:21987700-21987719 | MS.gene71317:intron | 45.0% | |
| CACCTGTTCAAGCATTTCTC+GGG | + | chr2.1:21988754-21988773 | MS.gene71317:CDS | 45.0% | |
| GATGAAAGAAGGGTCATCAG+TGG | - | chr2.1:21987523-21987542 | None:intergenic | 45.0% | |
| GCAGTCTAATACTGCTGATG+TGG | - | chr2.1:21988373-21988392 | None:intergenic | 45.0% | |
| TGCGGATAAAAGGACATGAG+AGG | - | chr2.1:21988887-21988906 | None:intergenic | 45.0% | |
| TGCTGTTCACATACTAGGGA+GGG | + | chr2.1:21987701-21987720 | MS.gene71317:intron | 45.0% | |
| ! | GCAGCGATTTGGATGAAAGA+AGG | - | chr2.1:21987534-21987553 | None:intergenic | 45.0% |
| GAAAGAAGGGTCATCAGTGG+AGG | - | chr2.1:21987520-21987539 | None:intergenic | 50.0% | |
| GGAGAACACTAGCAGCGATT+TGG | - | chr2.1:21987545-21987564 | None:intergenic | 50.0% | |
| GGTCAAGAATTGTTCCACCC+AGG | - | chr2.1:21987908-21987927 | None:intergenic | 50.0% | |
| TAAGTTCGCCACCATCATCG+AGG | + | chr2.1:21988812-21988831 | MS.gene71317:CDS | 50.0% | |
| TCAGTGGAGGAGAGAAGAGA+AGG | - | chr2.1:21987507-21987526 | None:intergenic | 50.0% | |
| TTTCTCGGGGCAATATCAGC+TGG | + | chr2.1:21988768-21988787 | MS.gene71317:CDS | 50.0% | |
| ! | GTTTGAGACGTTTAGCCCTG+CGG | - | chr2.1:21987608-21987627 | None:intergenic | 50.0% |
| GAGGAGGACGGAAATAGCAC+CGG | - | chr2.1:21987571-21987590 | None:intergenic | 55.0% | |
| GCCCCGAGAAATGCTTGAAC+AGG | - | chr2.1:21988759-21988778 | None:intergenic | 55.0% | |
| GCTGCTAGTGTTCTCCTCAC+CGG | + | chr2.1:21987549-21987568 | MS.gene71317:CDS | 55.0% | |
| GAAGGAGCGGAAGAGGAGGA+CGG | - | chr2.1:21987583-21987602 | None:intergenic | 60.0% | |
| GTTCGCCACCATCATCGAGG+CGG | + | chr2.1:21988815-21988834 | MS.gene71317:CDS | 60.0% | |
| TGAGACGTTTAGCCCTGCGG+CGG | - | chr2.1:21987605-21987624 | None:intergenic | 60.0% | |
| TTCGCCACCATCATCGAGGC+GGG | + | chr2.1:21988816-21988835 | MS.gene71317:CDS | 60.0% | |
| !! | GGACGGAAATAGCACCGGTG+AGG | - | chr2.1:21987566-21987585 | None:intergenic | 60.0% |
| ACGTTTAGCCCTGCGGCGGA+AGG | - | chr2.1:21987601-21987620 | None:intergenic | 65.0% | |
| GCTCAGACCCGCCTCGATGA+TGG | - | chr2.1:21988826-21988845 | None:intergenic | 65.0% | |
| TCATCGAGGCGGGTCTGAGC+CGG | + | chr2.1:21988826-21988845 | MS.gene71317:CDS | 65.0% | |
| ! | CAGACCCGCCTCGATGATGG+TGG | - | chr2.1:21988823-21988842 | None:intergenic | 65.0% |
| CTTCCGCTCCTTCCGCCGCA+GGG | + | chr2.1:21987590-21987609 | MS.gene71317:CDS | 70.0% | |
| GGCGGAAGGAGCGGAAGAGG+AGG | - | chr2.1:21987587-21987606 | None:intergenic | 70.0% | |
| TAGCCCTGCGGCGGAAGGAG+CGG | - | chr2.1:21987596-21987615 | None:intergenic | 70.0% | |
| TCTTCCGCTCCTTCCGCCGC+AGG | + | chr2.1:21987589-21987608 | MS.gene71317:CDS | 70.0% | |
| TGCGGCGGAAGGAGCGGAAG+AGG | - | chr2.1:21987590-21987609 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 21987463 | 21988974 | 21987463 | ID=MS.gene71317 |
| chr2.1 | mRNA | 21987463 | 21988974 | 21987463 | ID=MS.gene71317.t1;Parent=MS.gene71317 |
| chr2.1 | exon | 21987463 | 21987632 | 21987463 | ID=MS.gene71317.t1.exon1;Parent=MS.gene71317.t1 |
| chr2.1 | CDS | 21987463 | 21987632 | 21987463 | ID=cds.MS.gene71317.t1;Parent=MS.gene71317.t1 |
| chr2.1 | exon | 21988662 | 21988872 | 21988662 | ID=MS.gene71317.t1.exon2;Parent=MS.gene71317.t1 |
| chr2.1 | CDS | 21988662 | 21988872 | 21988662 | ID=cds.MS.gene71317.t1;Parent=MS.gene71317.t1 |
| chr2.1 | exon | 21988930 | 21988974 | 21988930 | ID=MS.gene71317.t1.exon3;Parent=MS.gene71317.t1 |
| chr2.1 | CDS | 21988930 | 21988974 | 21988930 | ID=cds.MS.gene71317.t1;Parent=MS.gene71317.t1 |
| Gene Sequence |
| Protein sequence |
