AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83095


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83095.t1 MTR_1g031740 81.928 83 9 1 1 83 76 152 4.62e-43 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGAGTGGCTGGGTGAAAA+TGG 0.232098 1.2:+16616795 MS.gene83095:CDS
TTGTGTTGGGTGGTTGGCTT+GGG 0.291830 1.2:-16617214 None:intergenic
TCTCGAGATCTACAAGTCTT+TGG 0.331857 1.2:+16617161 MS.gene83095:CDS
ATTGTGTTGGGTGGTTGGCT+TGG 0.342575 1.2:-16617215 None:intergenic
CCTGGATTGTGTTGGGTGGT+TGG 0.351530 1.2:-16617220 None:intergenic
GACCACGATGGATTTGTTCT+TGG 0.365556 1.2:+16616728 MS.gene83095:CDS
CCAACCACCCAACACAATCC+AGG 0.377108 1.2:+16617220 MS.gene83095:CDS
ACCACGATGGATTTGTTCTT+GGG 0.379690 1.2:+16616729 MS.gene83095:CDS
GAGAGTGGCTGGGTGAAAAT+GGG 0.391269 1.2:+16616796 MS.gene83095:CDS
GGTGTTAGTGGAGCGAAATC+TGG 0.391808 1.2:-16616823 None:intergenic
TTGCTACTTACTGGTGTTAG+TGG 0.404594 1.2:-16616835 None:intergenic
TGAAGGCCCTGGATTGTGTT+GGG 0.448909 1.2:-16617227 None:intergenic
GAGGGAAACAGGAGAGTGGC+TGG 0.452230 1.2:+16616785 MS.gene83095:CDS
CTGAAGGCCCTGGATTGTGT+TGG 0.456207 1.2:-16617228 None:intergenic
ATTGTTGAGGGAGAGGCAAT+AGG 0.476898 1.2:-16617255 None:intergenic
TTCTTGGGACAAGTATTTGA+AGG 0.485835 1.2:+16616744 MS.gene83095:CDS
ATTGAACGTGCCCTTGGCGC+TGG 0.500974 1.2:+16616495 MS.gene83095:CDS
AATAGGTCTGCTGAAGGCCC+TGG 0.505378 1.2:-16617238 None:intergenic
GTCTCATATTGTTGAGGGAG+AGG 0.509046 1.2:-16617262 None:intergenic
ATGCAAATTGAACGTGCCCT+TGG 0.511985 1.2:+16616489 MS.gene83095:CDS
GACAGAGCATACTTGAGGTC+AGG 0.519678 1.2:-16616537 None:intergenic
CTCTGAAGCTACCAGCGCCA+AGG 0.529782 1.2:-16616506 None:intergenic
CTAGATGTCTCATATTGTTG+AGG 0.544710 1.2:-16617268 None:intergenic
TAGATGTCTCATATTGTTGA+GGG 0.546163 1.2:-16617267 None:intergenic
CAACCACCCAACACAATCCA+GGG 0.567075 1.2:+16617221 MS.gene83095:CDS
TCTTGGGACAAGTATTTGAA+GGG 0.567555 1.2:+16616745 MS.gene83095:CDS
AGAGTGGCTGGGTGAAAATG+GGG 0.590303 1.2:+16616797 MS.gene83095:CDS
TCTGAAGCTACCAGCGCCAA+GGG 0.590855 1.2:-16616505 None:intergenic
CTCGAGACAAGCAATGGACG+AGG 0.601787 1.2:-16617145 None:intergenic
AGGGAAACAGGAGAGTGGCT+GGG 0.620803 1.2:+16616786 MS.gene83095:CDS
GGCGCTGGTAGCTTCAGAGA+CGG 0.635842 1.2:+16616510 MS.gene83095:CDS
AGAGGCAATAGGTCTGCTGA+AGG 0.636549 1.2:-16617244 None:intergenic
AGGCCCTGGATTGTGTTGGG+TGG 0.642633 1.2:-16617224 None:intergenic
GTAGATCTCGAGACAAGCAA+TGG 0.668961 1.2:-16617151 None:intergenic
AGCTGAGGGAAACAGGAGAG+TGG 0.671817 1.2:+16616781 MS.gene83095:CDS
TCCCAAGAACAAATCCATCG+TGG 0.736351 1.2:-16616730 None:intergenic
AGACGTAAAGAAGACCACGA+TGG 0.745068 1.2:+16616716 MS.gene83095:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATAACATTATGATGAAT+AGG + chr1.2:16616590-16616609 MS.gene83095:intron 15.0%
!!! TTGTTTTTTAAATAAGAAAC+TGG + chr1.2:16617024-16617043 MS.gene83095:intron 15.0%
!! TGTTAAAGAGACAAAAAATA+CGG - chr1.2:16616876-16616895 None:intergenic 20.0%
!!! CACTTTTAACATTATGAAAT+TGG - chr1.2:16616632-16616651 None:intergenic 20.0%
!!! TATTTTTTGTCTCTTTAACA+AGG + chr1.2:16616876-16616895 MS.gene83095:intron 20.0%
!!! TCTTTTGCTTTGTTATATAT+AGG + chr1.2:16616910-16616929 MS.gene83095:intron 20.0%
! AGATTATTACTTCTTACAAC+TGG - chr1.2:16617082-16617101 None:intergenic 25.0%
! ATCAAAATCCAGAATGAAAT+TGG - chr1.2:16616657-16616676 None:intergenic 25.0%
! GTTGTAAGAAGTAATAATCT+TGG + chr1.2:16617082-16617101 MS.gene83095:intron 25.0%
TAGATGTCTCATATTGTTGA+GGG - chr1.2:16617270-16617289 None:intergenic 30.0%
TTATATATAGGTGATGAGTG+AGG + chr1.2:16616922-16616941 MS.gene83095:intron 30.0%
CTAGATGTCTCATATTGTTG+AGG - chr1.2:16617271-16617290 None:intergenic 35.0%
GTGAGGAAAAACACAAGTTA+TGG + chr1.2:16616939-16616958 MS.gene83095:intron 35.0%
TCTTGGGACAAGTATTTGAA+GGG + chr1.2:16616745-16616764 MS.gene83095:CDS 35.0%
TTCTTGGGACAAGTATTTGA+AGG + chr1.2:16616744-16616763 MS.gene83095:CDS 35.0%
! AAAGTGCTCCAATTTCATTC+TGG + chr1.2:16616646-16616665 MS.gene83095:intron 35.0%
AAACAGACAGAGCATACTTG+AGG - chr1.2:16616545-16616564 None:intergenic 40.0%
TAACACCAGTAAGTAGCAAC+TGG + chr1.2:16616839-16616858 MS.gene83095:intron 40.0%
TCTCGAGATCTACAAGTCTT+TGG + chr1.2:16617161-16617180 MS.gene83095:CDS 40.0%
! ACCACGATGGATTTGTTCTT+GGG + chr1.2:16616729-16616748 MS.gene83095:CDS 40.0%
! TTGCTACTTACTGGTGTTAG+TGG - chr1.2:16616838-16616857 None:intergenic 40.0%
AGACGTAAAGAAGACCACGA+TGG + chr1.2:16616716-16616735 MS.gene83095:CDS 45.0%
ATGCAAATTGAACGTGCCCT+TGG + chr1.2:16616489-16616508 MS.gene83095:CDS 45.0%
ATTGTTGAGGGAGAGGCAAT+AGG - chr1.2:16617258-16617277 None:intergenic 45.0%
CAGAAAAAGCTGAGGGAAAC+AGG + chr1.2:16616774-16616793 MS.gene83095:CDS 45.0%
GACTGTGCAGAAAAAGCTGA+GGG + chr1.2:16616767-16616786 MS.gene83095:CDS 45.0%
GTAGATCTCGAGACAAGCAA+TGG - chr1.2:16617154-16617173 None:intergenic 45.0%
GTCTCATATTGTTGAGGGAG+AGG - chr1.2:16617265-16617284 None:intergenic 45.0%
TCCCAAGAACAAATCCATCG+TGG - chr1.2:16616733-16616752 None:intergenic 45.0%
! CGTTACCAGTTGCTACTTAC+TGG - chr1.2:16616847-16616866 None:intergenic 45.0%
! GACCACGATGGATTTGTTCT+TGG + chr1.2:16616728-16616747 MS.gene83095:CDS 45.0%
AGAGGCAATAGGTCTGCTGA+AGG - chr1.2:16617247-16617266 None:intergenic 50.0%
AGAGTGGCTGGGTGAAAATG+GGG + chr1.2:16616797-16616816 MS.gene83095:CDS 50.0%
CAACCACCCAACACAATCCA+GGG + chr1.2:16617221-16617240 MS.gene83095:CDS 50.0%
CGAGGCCTACAATTTAGCGA+AGG - chr1.2:16617130-16617149 None:intergenic 50.0%
CGAGGCCTTCGCTAAATTGT+AGG + chr1.2:16617122-16617141 MS.gene83095:intron 50.0%
GACAGAGCATACTTGAGGTC+AGG - chr1.2:16616540-16616559 None:intergenic 50.0%
GAGAGTGGCTGGGTGAAAAT+GGG + chr1.2:16616796-16616815 MS.gene83095:CDS 50.0%
GGACTGTGCAGAAAAAGCTG+AGG + chr1.2:16616766-16616785 MS.gene83095:CDS 50.0%
GGTGTTAGTGGAGCGAAATC+TGG - chr1.2:16616826-16616845 None:intergenic 50.0%
TGAAGGCCCTGGATTGTGTT+GGG - chr1.2:16617230-16617249 None:intergenic 50.0%
! ATTGTGTTGGGTGGTTGGCT+TGG - chr1.2:16617218-16617237 None:intergenic 50.0%
! GAGACTAGCTGATGAAGACG+AGG + chr1.2:16617104-16617123 MS.gene83095:intron 50.0%
!! TTGTGTTGGGTGGTTGGCTT+GGG - chr1.2:16617217-16617236 None:intergenic 50.0%
AATAGGTCTGCTGAAGGCCC+TGG - chr1.2:16617241-16617260 None:intergenic 55.0%
AGCTGAGGGAAACAGGAGAG+TGG + chr1.2:16616781-16616800 MS.gene83095:CDS 55.0%
AGGGAAACAGGAGAGTGGCT+GGG + chr1.2:16616786-16616805 MS.gene83095:CDS 55.0%
CCAACCACCCAACACAATCC+AGG + chr1.2:16617220-16617239 MS.gene83095:CDS 55.0%
CCTGGATTGTGTTGGGTGGT+TGG - chr1.2:16617223-16617242 None:intergenic 55.0%
CTCGAGACAAGCAATGGACG+AGG - chr1.2:16617148-16617167 None:intergenic 55.0%
CTGAAGGCCCTGGATTGTGT+TGG - chr1.2:16617231-16617250 None:intergenic 55.0%
GGAGAGTGGCTGGGTGAAAA+TGG + chr1.2:16616795-16616814 MS.gene83095:CDS 55.0%
! TCTGAAGCTACCAGCGCCAA+GGG - chr1.2:16616508-16616527 None:intergenic 55.0%
AGGCCCTGGATTGTGTTGGG+TGG - chr1.2:16617227-16617246 None:intergenic 60.0%
ATTGAACGTGCCCTTGGCGC+TGG + chr1.2:16616495-16616514 MS.gene83095:CDS 60.0%
GAGGGAAACAGGAGAGTGGC+TGG + chr1.2:16616785-16616804 MS.gene83095:CDS 60.0%
!! CTCTGAAGCTACCAGCGCCA+AGG - chr1.2:16616509-16616528 None:intergenic 60.0%
!! GGCGCTGGTAGCTTCAGAGA+CGG + chr1.2:16616510-16616529 MS.gene83095:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 16616489 16617301 16616489 ID=MS.gene83095
chr1.2 mRNA 16616489 16617301 16616489 ID=MS.gene83095.t1;Parent=MS.gene83095
chr1.2 exon 16616489 16616547 16616489 ID=MS.gene83095.t1.exon1;Parent=MS.gene83095.t1
chr1.2 CDS 16616489 16616547 16616489 ID=cds.MS.gene83095.t1;Parent=MS.gene83095.t1
chr1.2 exon 16616701 16616846 16616701 ID=MS.gene83095.t1.exon2;Parent=MS.gene83095.t1
chr1.2 CDS 16616701 16616846 16616701 ID=cds.MS.gene83095.t1;Parent=MS.gene83095.t1
chr1.2 exon 16617144 16617301 16617144 ID=MS.gene83095.t1.exon3;Parent=MS.gene83095.t1
chr1.2 CDS 16617144 16617301 16617144 ID=cds.MS.gene83095.t1;Parent=MS.gene83095.t1
Gene Sequence

>MS.gene83095

ATGCAAATTGAACGTGCCCTTGGCGCTGGTAGCTTCAGAGACGGTGAACCTGACCTCAAGTATGCTCTGTCTGTTTGAATAAACAACTTATTTTGCAGCTTATTATAACATTATGATGAATAGGCTATTTCTATTCACTACCAATTTCATAATGTTAAAAGTGCTCCAATTTCATTCTGGATTTTGATTTTTCTGCATTTTTTTTAAAAAAGAATGAAGAACGATTCAGACGTAAAGAAGACCACGATGGATTTGTTCTTGGGACAAGTATTTGAAGGGACTGTGCAGAAAAAGCTGAGGGAAACAGGAGAGTGGCTGGGTGAAAATGGGGAGACCAGATTTCGCTCCACTAACACCAGTAAGTAGCAACTGGTAACGACTTTTCCGTATTTTTTGTCTCTTTAACAAGGATTGTAGCATGTCTTTTGCTTTGTTATATATAGGTGATGAGTGAGGAAAAACACAAGTTATGGAGTAATAGTATGAATGATTAATTGAGCACATATCTTTATAGCATTTATTCTTGAGATAACATTTGTTTTTTAAATAAGAAACTGGATGCAATTTATTATCAATCAAAAATGAAATAACCAGTTGTAAGAAGTAATAATCTTGGAGACTAGCTGATGAAGACGAGGCCTTCGCTAAATTGTAGGCCTCGTCCATTGCTTGTCTCGAGATCTACAAGTCTTTGGCTATCTAGTTCAAACACTACATGATGCAAACCCAAGCCAACCACCCAACACAATCCAGGGCCTTCAGCAGACCTATTGCCTCTCCCTCAACAATATGAGACATCTAGTCAGTTCTTGA

Protein sequence

>MS.gene83095.t1

MQIERALGAGSFRDGEPDLKMKNDSDVKKTTMDLFLGQVFEGTVQKKLRETGEWLGENGETRFRSTNTSLVHCLSRDLQVFGYLVQTLHDANPSQPPNTIQGLQQTYCLSLNNMRHLVSS