Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0566960 | XP_004504125.1 | 85.366 | 82 | 12 | 0 | 7 | 88 | 2 | 83 | 1.05e-32 | 127 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0566960 | A0A1S2YFP5 | 85.366 | 82 | 12 | 0 | 7 | 88 | 2 | 83 | 5.02e-33 | 127 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0010770 | Msa0566960 | 0.810388 | 1.181538e-50 | -8.615850e-47 |
| Msa0017320 | Msa0566960 | 0.817560 | 3.095536e-52 | -8.615850e-47 |
| Msa0031180 | Msa0566960 | 0.801627 | 8.233568e-49 | -8.615850e-47 |
| Msa0042550 | Msa0566960 | 0.806588 | 7.649373e-50 | -8.615850e-47 |
| Msa0043500 | Msa0566960 | 0.820735 | 5.860685e-53 | -8.615850e-47 |
| Msa0043650 | Msa0566960 | 0.820519 | 6.572688e-53 | -8.615850e-47 |
| Msa0045340 | Msa0566960 | 0.806077 | 9.799019e-50 | -8.615850e-47 |
| Msa0045550 | Msa0566960 | 0.822771 | 1.982428e-53 | -8.615850e-47 |
| Msa0054820 | Msa0566960 | 0.837838 | 4.103383e-57 | -8.615850e-47 |
| Msa0055070 | Msa0566960 | 0.804623 | 1.976386e-49 | -8.615850e-47 |
| Msa0056450 | Msa0566960 | 0.813186 | 2.907411e-51 | -8.615850e-47 |
| Msa0078480 | Msa0566960 | 0.801333 | 9.458854e-49 | -8.615850e-47 |
| Msa0080830 | Msa0566960 | 0.809310 | 2.015301e-50 | -8.615850e-47 |
| Msa0096580 | Msa0566960 | 0.818977 | 1.479040e-52 | -8.615850e-47 |
| Msa0100160 | Msa0566960 | 0.822390 | 2.430661e-53 | -8.615850e-47 |
| Msa0101020 | Msa0566960 | 0.802955 | 4.387558e-49 | -8.615850e-47 |
| Msa0103250 | Msa0566960 | 0.807917 | 3.999742e-50 | -8.615850e-47 |
| Msa0106620 | Msa0566960 | 0.808447 | 3.083937e-50 | -8.615850e-47 |
| Msa0106710 | Msa0566960 | 0.805009 | 1.641375e-49 | -8.615850e-47 |
| Msa0168870 | Msa0566960 | 0.806080 | 9.787365e-50 | -8.615850e-47 |
| Msa0174260 | Msa0566960 | 0.805868 | 1.084192e-49 | -8.615850e-47 |
| Msa0178510 | Msa0566960 | 0.818934 | 1.512249e-52 | -8.615850e-47 |
| Msa0183420 | Msa0566960 | 0.817358 | 3.437684e-52 | -8.615850e-47 |
| Msa0183760 | Msa0566960 | 0.800067 | 1.715107e-48 | -8.615850e-47 |
| Msa0186950 | Msa0566960 | 0.808459 | 3.065516e-50 | -8.615850e-47 |
| Msa0218350 | Msa0566960 | 0.805264 | 1.451774e-49 | -8.615850e-47 |
| Msa0220730 | Msa0566960 | 0.809356 | 1.970613e-50 | -8.615850e-47 |
| Msa0227230 | Msa0566960 | 0.806042 | 9.968921e-50 | -8.615850e-47 |
| Msa0232340 | Msa0566960 | 0.805479 | 1.308434e-49 | -8.615850e-47 |
| Msa0237200 | Msa0566960 | 0.815641 | 8.330490e-52 | -8.615850e-47 |
| Msa0237210 | Msa0566960 | 0.817285 | 3.570523e-52 | -8.615850e-47 |
| Msa0378970 | Msa0566960 | 0.825032 | 5.847676e-54 | -8.615850e-47 |
| Msa0382370 | Msa0566960 | 0.825498 | 4.536819e-54 | -8.615850e-47 |
| Msa0387050 | Msa0566960 | 0.813250 | 2.814375e-51 | -8.615850e-47 |
| Msa0389910 | Msa0566960 | 0.801575 | 8.441584e-49 | -8.615850e-47 |
| Msa0390530 | Msa0566960 | 0.810939 | 8.980846e-51 | -8.615850e-47 |
| Msa0394690 | Msa0566960 | 0.827603 | 1.427810e-54 | -8.615850e-47 |
| Msa0395980 | Msa0566960 | 0.821234 | 4.499507e-53 | -8.615850e-47 |
| Msa0398200 | Msa0566960 | 0.811587 | 6.497086e-51 | -8.615850e-47 |
| Msa0428580 | Msa0566960 | 0.812667 | 3.777631e-51 | -8.615850e-47 |
| Msa0432160 | Msa0566960 | 0.827518 | 1.496778e-54 | -8.615850e-47 |
| Msa0440510 | Msa0566960 | 0.813765 | 2.169256e-51 | -8.615850e-47 |
| Msa0443430 | Msa0566960 | 0.801325 | 9.495175e-49 | -8.615850e-47 |
| Msa0468320 | Msa0566960 | 0.803465 | 3.441799e-49 | -8.615850e-47 |
| Msa0473980 | Msa0566960 | 0.812296 | 4.553584e-51 | -8.615850e-47 |
| Msa0474940 | Msa0566960 | 0.813872 | 2.054610e-51 | -8.615850e-47 |
| Msa0476680 | Msa0566960 | 0.828415 | 9.105779e-55 | -8.615850e-47 |
| Msa0480780 | Msa0566960 | 0.828340 | 9.490676e-55 | -8.615850e-47 |
| Msa0481970 | Msa0566960 | 0.807387 | 5.181504e-50 | -8.615850e-47 |
| Msa0482180 | Msa0566960 | 0.811571 | 6.550173e-51 | -8.615850e-47 |
| Msa0240270 | Msa0566960 | 0.812258 | 4.640646e-51 | -8.615850e-47 |
| Msa0271790 | Msa0566960 | 0.838051 | 3.617880e-57 | -8.615850e-47 |
| Msa0274970 | Msa0566960 | 0.818942 | 1.506566e-52 | -8.615850e-47 |
| Msa0274980 | Msa0566960 | 0.803779 | 2.961924e-49 | -8.615850e-47 |
| Msa0278580 | Msa0566960 | 0.800772 | 1.232228e-48 | -8.615850e-47 |
| Msa0303460 | Msa0566960 | 0.818345 | 2.057077e-52 | -8.615850e-47 |
| Msa0303520 | Msa0566960 | 0.823074 | 1.684518e-53 | -8.615850e-47 |
| Msa0307310 | Msa0566960 | 0.801560 | 8.501189e-49 | -8.615850e-47 |
| Msa0310430 | Msa0566960 | 0.829260 | 5.684815e-55 | -8.615850e-47 |
| Msa0315680 | Msa0566960 | 0.820561 | 6.428366e-53 | -8.615850e-47 |
| Msa0315690 | Msa0566960 | 0.813135 | 2.984040e-51 | -8.615850e-47 |
| Msa0317960 | Msa0566960 | 0.807814 | 4.206276e-50 | -8.615850e-47 |
| Msa0341230 | Msa0566960 | 0.801787 | 7.634536e-49 | -8.615850e-47 |
| Msa0347950 | Msa0566960 | 0.837168 | 6.096055e-57 | -8.615850e-47 |
| Msa0347990 | Msa0566960 | 0.846084 | 2.711865e-59 | -8.615850e-47 |
| Msa0482940 | Msa0566960 | 0.801377 | 9.267445e-49 | -8.615850e-47 |
| Msa0485490 | Msa0566960 | 0.803673 | 3.115385e-49 | -8.615850e-47 |
| Msa0489660 | Msa0566960 | 0.820272 | 7.485894e-53 | -8.615850e-47 |
| Msa0491000 | Msa0566960 | 0.819482 | 1.134746e-52 | -8.615850e-47 |
| Msa0514610 | Msa0566960 | 0.804069 | 2.578202e-49 | -8.615850e-47 |
| Msa0515710 | Msa0566960 | 0.800583 | 1.346480e-48 | -8.615850e-47 |
| Msa0521010 | Msa0566960 | 0.825631 | 4.219764e-54 | -8.615850e-47 |
| Msa0526020 | Msa0566960 | 0.813751 | 2.184211e-51 | -8.615850e-47 |
| Msa0559500 | Msa0566960 | 0.802704 | 4.945549e-49 | -8.615850e-47 |
| Msa0560500 | Msa0566960 | 0.800737 | 1.252263e-48 | -8.615850e-47 |
| Msa0566350 | Msa0566960 | 0.818443 | 1.955018e-52 | -8.615850e-47 |
| Msa0566750 | Msa0566960 | 0.831180 | 1.931512e-55 | -8.615850e-47 |
| Msa0566960 | Msa0594200 | 0.810165 | 1.319801e-50 | -8.615850e-47 |
| Msa0566960 | Msa0600450 | 0.803198 | 3.909008e-49 | -8.615850e-47 |
| Msa0566960 | Msa0610250 | 0.819236 | 1.291504e-52 | -8.615850e-47 |
| Msa0566960 | Msa0618950 | 0.805679 | 1.188398e-49 | -8.615850e-47 |
| Msa0566960 | Msa0618970 | 0.803331 | 3.669247e-49 | -8.615850e-47 |
| Msa0566960 | Msa0622120 | 0.813187 | 2.905421e-51 | -8.615850e-47 |
| Msa0566960 | Msa0624060 | 0.806330 | 8.667238e-50 | -8.615850e-47 |
| Msa0566960 | Msa0638350 | 0.810144 | 1.333667e-50 | -8.615850e-47 |
| Msa0566960 | Msa0638360 | 0.819555 | 1.091964e-52 | -8.615850e-47 |
| Msa0566960 | Msa0640940 | 0.807459 | 5.003176e-50 | -8.615850e-47 |
| Msa0566960 | Msa0641730 | 0.824020 | 1.012058e-53 | -8.615850e-47 |
| Msa0566960 | Msa0652690 | 0.829469 | 5.058816e-55 | -8.615850e-47 |
| Msa0566960 | Msa0658680 | 0.816451 | 5.491698e-52 | -8.615850e-47 |
| Msa0566960 | Msa0661320 | 0.806485 | 8.041097e-50 | -8.615850e-47 |
| Msa0566960 | Msa0679360 | 0.802697 | 4.962090e-49 | -8.615850e-47 |
| Msa0566960 | Msa0689760 | 0.816018 | 6.865474e-52 | -8.615850e-47 |
| Msa0566960 | Msa0703440 | 0.831207 | 1.902121e-55 | -8.615850e-47 |
| Msa0566960 | Msa0705390 | 0.814815 | 1.271495e-51 | -8.615850e-47 |
| Msa0566960 | Msa0705990 | 0.804507 | 2.089556e-49 | -8.615850e-47 |
| Msa0566960 | Msa0710270 | 0.802085 | 6.630980e-49 | -8.615850e-47 |
| Msa0566960 | Msa0713870 | 0.813798 | 2.132308e-51 | -8.615850e-47 |
| Msa0566960 | Msa0716860 | 0.800438 | 1.441269e-48 | -8.615850e-47 |
| Msa0566960 | Msa0721340 | 0.810520 | 1.106803e-50 | -8.615850e-47 |
| Msa0566960 | Msa0732600 | 0.822027 | 2.950161e-53 | -8.615850e-47 |
| Msa0566960 | Msa0743530 | 0.800519 | 1.387333e-48 | -8.615850e-47 |
| Msa0566960 | Msa0749440 | 0.803332 | 3.666877e-49 | -8.615850e-47 |
| Msa0566960 | Msa0750250 | 0.815116 | 1.089867e-51 | -8.615850e-47 |
| Msa0566960 | Msa0756380 | 0.804226 | 2.391179e-49 | -8.615850e-47 |
| Msa0566960 | Msa0758250 | 0.807814 | 4.205933e-50 | -8.615850e-47 |
| Msa0566960 | Msa0763890 | 0.803671 | 3.119422e-49 | -8.615850e-47 |
| Msa0566960 | Msa0763920 | 0.809856 | 1.538837e-50 | -8.615850e-47 |
| Msa0566960 | Msa0779260 | 0.813938 | 1.986317e-51 | -8.615850e-47 |
| Msa0566960 | Msa0789560 | 0.808469 | 3.050093e-50 | -8.615850e-47 |
| Msa0566960 | Msa0811040 | 0.801991 | 6.934640e-49 | -8.615850e-47 |
| Msa0566960 | Msa0818890 | 0.801131 | 1.040590e-48 | -8.615850e-47 |
| Msa0566960 | Msa0866400 | 0.845070 | 5.106684e-59 | -8.615850e-47 |
| Msa0566960 | Msa0866410 | 0.831973 | 1.232216e-55 | -8.615850e-47 |
| Msa0566960 | Msa0868160 | 0.805191 | 1.503901e-49 | -8.615850e-47 |
| Msa0566960 | Msa0873860 | 0.806292 | 8.828253e-50 | -8.615850e-47 |
| Msa0566960 | Msa0903780 | 0.814070 | 1.857388e-51 | -8.615850e-47 |
| Msa0566960 | Msa0905750 | 0.802750 | 4.837926e-49 | -8.615850e-47 |
| Msa0566960 | Msa0937430 | 0.807859 | 4.114873e-50 | -8.615850e-47 |
| Msa0566960 | Msa0945240 | 0.830086 | 3.580000e-55 | -8.615850e-47 |
| Msa0566960 | Msa0958030 | 0.816297 | 5.947048e-52 | -8.615850e-47 |
| Msa0566960 | Msa0985180 | 0.800059 | 1.721645e-48 | -8.615850e-47 |
| Msa0566960 | Msa0986510 | 0.802077 | 6.658345e-49 | -8.615850e-47 |
| Msa0566960 | Msa0992580 | 0.818763 | 1.654319e-52 | -8.615850e-47 |
| Msa0566960 | Msa0992600 | 0.819974 | 8.761052e-53 | -8.615850e-47 |
| Msa0566960 | Msa1008750 | 0.819458 | 1.149371e-52 | -8.615850e-47 |
| Msa0566960 | Msa1011090 | 0.824918 | 6.220864e-54 | -8.615850e-47 |
| Msa0566960 | Msa1034500 | 0.803641 | 3.164165e-49 | -8.615850e-47 |
| Msa0566960 | Msa1039710 | -0.803121 | 4.055512e-49 | -8.615850e-47 |
| Msa0566960 | Msa1042660 | 0.805378 | 1.374215e-49 | -8.615850e-47 |
| Msa0566960 | Msa1063420 | 0.826582 | 2.506318e-54 | -8.615850e-47 |
| Msa0566960 | Msa1077070 | 0.800415 | 1.456924e-48 | -8.615850e-47 |
| Msa0566960 | Msa1088410 | 0.822194 | 2.699373e-53 | -8.615850e-47 |
| Msa0566960 | Msa1098270 | 0.803263 | 3.789843e-49 | -8.615850e-47 |
| Msa0566960 | Msa1127430 | 0.818435 | 1.963328e-52 | -8.615850e-47 |
| Msa0566960 | Msa1130530 | 0.821687 | 3.537339e-53 | -8.615850e-47 |
| Msa0566960 | Msa1143020 | 0.800239 | 1.582323e-48 | -8.615850e-47 |
| Msa0566960 | Msa1205590 | 0.806231 | 9.093240e-50 | -8.615850e-47 |
| Msa0566960 | Msa1220940 | 0.802808 | 4.706289e-49 | -8.615850e-47 |
| Msa0566960 | Msa1253450 | 0.803380 | 3.583225e-49 | -8.615850e-47 |
| Msa0566960 | Msa1264420 | 0.830319 | 3.140853e-55 | -8.615850e-47 |
| Msa0566960 | Msa1270660 | 0.824926 | 6.192531e-54 | -8.615850e-47 |
| Msa0566960 | Msa1286550 | 0.816698 | 4.836126e-52 | -8.615850e-47 |
| Msa0566960 | Msa1297530 | 0.818020 | 2.436805e-52 | -8.615850e-47 |
| Msa0566960 | Msa1304940 | 0.809004 | 2.344725e-50 | -8.615850e-47 |
| Msa0566960 | Msa1332050 | 0.801446 | 8.969199e-49 | -8.615850e-47 |
| Msa0566960 | Msa1336040 | 0.823756 | 1.167275e-53 | -8.615850e-47 |
| Msa0566960 | Msa1341490 | 0.818702 | 1.707892e-52 | -8.615850e-47 |
| Msa0566960 | Msa1359560 | 0.828988 | 6.618262e-55 | -8.615850e-47 |
| Msa0566960 | Msa1359920 | 0.800141 | 1.656302e-48 | -8.615850e-47 |
| Msa0566960 | Msa1370590 | 0.801041 | 1.085703e-48 | -8.615850e-47 |
| Msa0566960 | Msa1371940 | 0.801328 | 9.483053e-49 | -8.615850e-47 |
| Msa0566960 | Msa1372150 | 0.815489 | 9.007856e-52 | -8.615850e-47 |
| Msa0566960 | Msa1372290 | 0.834447 | 2.981759e-56 | -8.615850e-47 |
| Msa0566960 | Msa1378410 | 0.809270 | 2.055454e-50 | -8.615850e-47 |
| Msa0566960 | Msa1384100 | 0.802354 | 5.838911e-49 | -8.615850e-47 |
| Msa0566960 | Msa1400120 | 0.838772 | 2.358045e-57 | -8.615850e-47 |
| Msa0566960 | Msa1400930 | 0.803848 | 2.865648e-49 | -8.615850e-47 |
| Msa0566960 | Msa1401460 | 0.807710 | 4.426224e-50 | -8.615850e-47 |
| Msa0566960 | Msa1416260 | 0.801283 | 9.685043e-49 | -8.615850e-47 |
| Msa0566960 | Msa1420250 | 0.810711 | 1.006426e-50 | -8.615850e-47 |
| Msa0566960 | Msa1426570 | 0.816680 | 4.880298e-52 | -8.615850e-47 |
| Msa0566960 | Msa1426910 | 0.807355 | 5.264664e-50 | -8.615850e-47 |
| Msa0566960 | Msa1428780 | 0.805469 | 1.315139e-49 | -8.615850e-47 |
| Msa0566960 | Msa1438520 | 0.812099 | 5.025927e-51 | -8.615850e-47 |
| Msa0566960 | Msa1442790 | 0.809178 | 2.151080e-50 | -8.615850e-47 |
| Msa0566960 | Msa1444120 | 0.803373 | 3.596474e-49 | -8.615850e-47 |
| Msa0566960 | Msa1450720 | 0.822116 | 2.813407e-53 | -8.615850e-47 |
| Msa0566960 | Msa1454960 | 0.802137 | 6.471752e-49 | -8.615850e-47 |
| Msa0566960 | Msa1458340 | 0.808864 | 2.511824e-50 | -8.615850e-47 |
| Msa0566960 | Msa1463030 | 0.814480 | 1.508044e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0566960 | MtrunA17_Chr8g0381611 | 90.244 | 82 | 8 | 0 | 7 | 88 | 2 | 83 | 1.52e-35 | 124 |
| Msa0566960 | MtrunA17_Chr3g0123571 | 48.913 | 92 | 43 | 1 | 1 | 88 | 1 | 92 | 2.60e-21 | 85.9 |
| Msa0566960 | MtrunA17_Chr8g0381601 | 50.000 | 88 | 39 | 2 | 5 | 88 | 5 | 91 | 2.07e-17 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0566960 | AT1G13000.2 | 48.000 | 50 | 26 | 0 | 11 | 60 | 15 | 64 | 3.39e-12 | 60.8 |
| Msa0566960 | AT1G13000.5 | 48.000 | 50 | 26 | 0 | 11 | 60 | 15 | 64 | 3.39e-12 | 60.8 |
| Msa0566960 | AT1G13000.4 | 48.000 | 50 | 26 | 0 | 11 | 60 | 15 | 64 | 3.39e-12 | 60.8 |
| Msa0566960 | AT1G13000.1 | 48.000 | 50 | 26 | 0 | 11 | 60 | 15 | 64 | 3.39e-12 | 60.8 |
| Msa0566960 | AT1G13000.3 | 48.000 | 50 | 26 | 0 | 11 | 60 | 15 | 64 | 3.47e-12 | 60.5 |
| Msa0566960 | AT3G27470.5 | 45.283 | 53 | 29 | 0 | 11 | 63 | 2 | 54 | 1.01e-11 | 59.3 |
| Msa0566960 | AT3G27470.2 | 45.283 | 53 | 29 | 0 | 11 | 63 | 2 | 54 | 1.25e-11 | 58.9 |
| Msa0566960 | AT3G27470.1 | 45.283 | 53 | 29 | 0 | 11 | 63 | 2 | 54 | 1.25e-11 | 58.9 |
| Msa0566960 | AT3G27470.4 | 45.283 | 53 | 29 | 0 | 11 | 63 | 2 | 54 | 1.25e-11 | 58.9 |
| Msa0566960 | AT3G27470.3 | 45.283 | 53 | 29 | 0 | 11 | 63 | 2 | 54 | 1.25e-11 | 58.9 |
Find 31 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTATCTTCTTTGCACTTTA+AGG | 0.065968 | 4_1:+68194922 | Msa0566960:five_prime_UTR |
| GGATTTCTTGGCTTATTTAT+AGG | 0.256210 | 4_1:+68195199 | Msa0566960:CDS |
| AAGTAACTTGCGGAATTGTT+AGG | 0.285302 | 4_1:+68194704 | Msa0566960:five_prime_UTR |
| AATTCCGCAAGTTACTTATA+TGG | 0.292250 | 4_1:-68194698 | None:intergenic |
| AGTAACTTGCGGAATTGTTA+GGG | 0.398520 | 4_1:+68194705 | Msa0566960:five_prime_UTR |
| TGTAAGATTCAAACCTTAGC+TGG | 0.404456 | 4_1:-68195484 | None:intergenic |
| CCTTTGTTGTTGAGAATGTT+GGG | 0.436329 | 4_1:-68195231 | None:intergenic |
| ACCTTTGTTGTTGAGAATGT+TGG | 0.462834 | 4_1:-68195232 | None:intergenic |
| ATGGAAGGAAGCAAGCTAGA+TGG | 0.497446 | 4_1:-68195373 | None:intergenic |
| TACTTCCATTGTTTCAACAT+TGG | 0.501002 | 4_1:+68194755 | Msa0566960:five_prime_UTR |
| TCTATCATGCATTCGCGGTA+GGG | 0.544163 | 4_1:+68195399 | Msa0566960:CDS |
| GTTACTGAATAATCCAGCTA+AGG | 0.547809 | 4_1:+68195471 | Msa0566960:CDS |
| TATAAGTGTAACACAAGGAA+AGG | 0.549306 | 4_1:-68194818 | None:intergenic |
| ATCTATCATGCATTCGCGGT+AGG | 0.556802 | 4_1:+68195398 | Msa0566960:CDS |
| ATCTTCTTTGCACTTTAAGG+AGG | 0.556864 | 4_1:+68194925 | Msa0566960:five_prime_UTR |
| AGCAACCAATGTTGAAACAA+TGG | 0.559493 | 4_1:-68194760 | None:intergenic |
| GCGAATGCATGATAGATCAA+TGG | 0.574728 | 4_1:-68195392 | None:intergenic |
| ATTGATCTATCATGCATTCG+CGG | 0.575875 | 4_1:+68195394 | Msa0566960:CDS |
| TAAAGCATGGTAGAAAGAAG+AGG | 0.583594 | 4_1:-68194842 | None:intergenic |
| CAAGAAGAAATATTAAAGCA+TGG | 0.607585 | 4_1:-68194855 | None:intergenic |
| GCATTCGCGGTAGGGATGCG+TGG | 0.610723 | 4_1:+68195407 | Msa0566960:CDS |
| TAACTTGCGGAATTGTTAGG+GGG | 0.623417 | 4_1:+68194707 | Msa0566960:five_prime_UTR |
| TAATGAGGGAAGTTACTCAC+AGG | 0.624857 | 4_1:+68195015 | Msa0566960:exon |
| ATGCATGATAGATCAATGGA+AGG | 0.632828 | 4_1:-68195388 | None:intergenic |
| CCCAACATTCTCAACAACAA+AGG | 0.638927 | 4_1:+68195231 | Msa0566960:CDS |
| GTAACTTGCGGAATTGTTAG+GGG | 0.647831 | 4_1:+68194706 | Msa0566960:five_prime_UTR |
| GGAAAGCAGTAGCTAGCACA+AGG | 0.658045 | 4_1:-68194797 | None:intergenic |
| AAATAAAATACGCAACCGCG+TGG | 0.661048 | 4_1:-68194664 | None:intergenic |
| AGGGCCATATAAGTAACTTG+CGG | 0.674191 | 4_1:+68194694 | Msa0566960:five_prime_UTR |
| GAGGTTATAAGTGTAACACA+AGG | 0.731220 | 4_1:-68194823 | None:intergenic |
| ATTTAATAGTGGATACCACG+CGG | 0.792049 | 4_1:+68194649 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAAAAAATTAGCA+AGG | - | chr4_1:68195341-68195360 | None:intergenic | 10.0% |
| !! | AAAAAAAAAAAAAATTAGCA+AGG | - | chr4_1:68195341-68195360 | None:intergenic | 10.0% |
| !!! | CGTATTTTATTTTATCAAAA+GGG | + | chr4_1:68194675-68194694 | Msa0566960:five_prime_UTR | 15.0% |
| !! | AGGATACAATAAAAAAAAAT+TGG | + | chr4_1:68194989-68195008 | Msa0566960:five_prime_UTR | 15.0% |
| !!! | CGTATTTTATTTTATCAAAA+GGG | + | chr4_1:68194675-68194694 | Msa0566960:five_prime_UTR | 15.0% |
| !! | AGGATACAATAAAAAAAAAT+TGG | + | chr4_1:68194989-68195008 | Msa0566960:five_prime_UTR | 15.0% |
| !!! | GCGTATTTTATTTTATCAAA+AGG | + | chr4_1:68194674-68194693 | Msa0566960:five_prime_UTR | 20.0% |
| !! | AAAAAAAATTGGACATAATG+AGG | + | chr4_1:68195000-68195019 | Msa0566960:exon | 20.0% |
| !! | AAAAAAATTGGACATAATGA+GGG | + | chr4_1:68195001-68195020 | Msa0566960:exon | 20.0% |
| !! | AGAAGCAAATATTTACTTAA+AGG | - | chr4_1:68195109-68195128 | None:intergenic | 20.0% |
| !! | AGTAAATATTTGCTTCTTTA+TGG | + | chr4_1:68195112-68195131 | Msa0566960:intron | 20.0% |
| !! | ATATGAATAAAAATTCACTG+AGG | - | chr4_1:68195284-68195303 | None:intergenic | 20.0% |
| !!! | GCGTATTTTATTTTATCAAA+AGG | + | chr4_1:68194674-68194693 | Msa0566960:five_prime_UTR | 20.0% |
| !! | AAAAAAAATTGGACATAATG+AGG | + | chr4_1:68195000-68195019 | Msa0566960:exon | 20.0% |
| !! | AAAAAAATTGGACATAATGA+GGG | + | chr4_1:68195001-68195020 | Msa0566960:exon | 20.0% |
| !! | AGAAGCAAATATTTACTTAA+AGG | - | chr4_1:68195109-68195128 | None:intergenic | 20.0% |
| !! | AGTAAATATTTGCTTCTTTA+TGG | + | chr4_1:68195112-68195131 | Msa0566960:intron | 20.0% |
| !! | ATATGAATAAAAATTCACTG+AGG | - | chr4_1:68195284-68195303 | None:intergenic | 20.0% |
| ! | CAAGAAGAAATATTAAAGCA+TGG | - | chr4_1:68194858-68194877 | None:intergenic | 25.0% |
| !! | TGTTCATTTTACAAACATCT+TGG | + | chr4_1:68194953-68194972 | Msa0566960:five_prime_UTR | 25.0% |
| !!! | AACATCTTGGTTTTCTTATA+TGG | + | chr4_1:68194966-68194985 | Msa0566960:five_prime_UTR | 25.0% |
| ! | TAGCATATGCAAAAATTCTT+AGG | - | chr4_1:68195078-68195097 | None:intergenic | 25.0% |
| !! | GAAAAACAAATGAAAGTATG+AGG | + | chr4_1:68195152-68195171 | Msa0566960:CDS | 25.0% |
| !!! | CTTGTTGTGATATTTTTCTT+TGG | + | chr4_1:68195175-68195194 | Msa0566960:CDS | 25.0% |
| ! | CAAGAAGAAATATTAAAGCA+TGG | - | chr4_1:68194858-68194877 | None:intergenic | 25.0% |
| !! | TGTTCATTTTACAAACATCT+TGG | + | chr4_1:68194953-68194972 | Msa0566960:five_prime_UTR | 25.0% |
| !!! | AACATCTTGGTTTTCTTATA+TGG | + | chr4_1:68194966-68194985 | Msa0566960:five_prime_UTR | 25.0% |
| ! | TAGCATATGCAAAAATTCTT+AGG | - | chr4_1:68195078-68195097 | None:intergenic | 25.0% |
| !! | GAAAAACAAATGAAAGTATG+AGG | + | chr4_1:68195152-68195171 | Msa0566960:CDS | 25.0% |
| !!! | CTTGTTGTGATATTTTTCTT+TGG | + | chr4_1:68195175-68195194 | Msa0566960:CDS | 25.0% |
| AATTCCGCAAGTTACTTATA+TGG | - | chr4_1:68194701-68194720 | None:intergenic | 30.0% | |
| TACTTCCATTGTTTCAACAT+TGG | + | chr4_1:68194755-68194774 | Msa0566960:five_prime_UTR | 30.0% | |
| TATAAGTGTAACACAAGGAA+AGG | - | chr4_1:68194821-68194840 | None:intergenic | 30.0% | |
| TCTATCTTCTTTGCACTTTA+AGG | + | chr4_1:68194922-68194941 | Msa0566960:five_prime_UTR | 30.0% | |
| !! | ATCTTGGTTTTCTTATATGG+AGG | + | chr4_1:68194969-68194988 | Msa0566960:five_prime_UTR | 30.0% |
| ! | GTTGTGATATTTTTCTTTGG+AGG | + | chr4_1:68195178-68195197 | Msa0566960:CDS | 30.0% |
| !! | TTTTTCTTTGGAGGATTTCT+TGG | + | chr4_1:68195187-68195206 | Msa0566960:CDS | 30.0% |
| GGATTTCTTGGCTTATTTAT+AGG | + | chr4_1:68195199-68195218 | Msa0566960:CDS | 30.0% | |
| AATTCCGCAAGTTACTTATA+TGG | - | chr4_1:68194701-68194720 | None:intergenic | 30.0% | |
| TACTTCCATTGTTTCAACAT+TGG | + | chr4_1:68194755-68194774 | Msa0566960:five_prime_UTR | 30.0% | |
| TATAAGTGTAACACAAGGAA+AGG | - | chr4_1:68194821-68194840 | None:intergenic | 30.0% | |
| TCTATCTTCTTTGCACTTTA+AGG | + | chr4_1:68194922-68194941 | Msa0566960:five_prime_UTR | 30.0% | |
| !! | ATCTTGGTTTTCTTATATGG+AGG | + | chr4_1:68194969-68194988 | Msa0566960:five_prime_UTR | 30.0% |
| ! | GTTGTGATATTTTTCTTTGG+AGG | + | chr4_1:68195178-68195197 | Msa0566960:CDS | 30.0% |
| !! | TTTTTCTTTGGAGGATTTCT+TGG | + | chr4_1:68195187-68195206 | Msa0566960:CDS | 30.0% |
| GGATTTCTTGGCTTATTTAT+AGG | + | chr4_1:68195199-68195218 | Msa0566960:CDS | 30.0% | |
| ! | AAGTAACTTGCGGAATTGTT+AGG | + | chr4_1:68194704-68194723 | Msa0566960:five_prime_UTR | 35.0% |
| ! | AGTAACTTGCGGAATTGTTA+GGG | + | chr4_1:68194705-68194724 | Msa0566960:five_prime_UTR | 35.0% |
| AGCAACCAATGTTGAAACAA+TGG | - | chr4_1:68194763-68194782 | None:intergenic | 35.0% | |
| GAGGTTATAAGTGTAACACA+AGG | - | chr4_1:68194826-68194845 | None:intergenic | 35.0% | |
| TAAAGCATGGTAGAAAGAAG+AGG | - | chr4_1:68194845-68194864 | None:intergenic | 35.0% | |
| ! | ATCTTCTTTGCACTTTAAGG+AGG | + | chr4_1:68194925-68194944 | Msa0566960:five_prime_UTR | 35.0% |
| !! | CCTTTGTTGTTGAGAATGTT+GGG | - | chr4_1:68195234-68195253 | None:intergenic | 35.0% |
| !! | ACCTTTGTTGTTGAGAATGT+TGG | - | chr4_1:68195235-68195254 | None:intergenic | 35.0% |
| ATGCATGATAGATCAATGGA+AGG | - | chr4_1:68195391-68195410 | None:intergenic | 35.0% | |
| ATTGATCTATCATGCATTCG+CGG | + | chr4_1:68195394-68195413 | Msa0566960:CDS | 35.0% | |
| GTTACTGAATAATCCAGCTA+AGG | + | chr4_1:68195471-68195490 | Msa0566960:CDS | 35.0% | |
| TGTAAGATTCAAACCTTAGC+TGG | - | chr4_1:68195487-68195506 | None:intergenic | 35.0% | |
| ! | AAGTAACTTGCGGAATTGTT+AGG | + | chr4_1:68194704-68194723 | Msa0566960:five_prime_UTR | 35.0% |
| ! | AGTAACTTGCGGAATTGTTA+GGG | + | chr4_1:68194705-68194724 | Msa0566960:five_prime_UTR | 35.0% |
| AGCAACCAATGTTGAAACAA+TGG | - | chr4_1:68194763-68194782 | None:intergenic | 35.0% | |
| GAGGTTATAAGTGTAACACA+AGG | - | chr4_1:68194826-68194845 | None:intergenic | 35.0% | |
| TAAAGCATGGTAGAAAGAAG+AGG | - | chr4_1:68194845-68194864 | None:intergenic | 35.0% | |
| ! | ATCTTCTTTGCACTTTAAGG+AGG | + | chr4_1:68194925-68194944 | Msa0566960:five_prime_UTR | 35.0% |
| !! | CCTTTGTTGTTGAGAATGTT+GGG | - | chr4_1:68195234-68195253 | None:intergenic | 35.0% |
| !! | ACCTTTGTTGTTGAGAATGT+TGG | - | chr4_1:68195235-68195254 | None:intergenic | 35.0% |
| ATGCATGATAGATCAATGGA+AGG | - | chr4_1:68195391-68195410 | None:intergenic | 35.0% | |
| ATTGATCTATCATGCATTCG+CGG | + | chr4_1:68195394-68195413 | Msa0566960:CDS | 35.0% | |
| GTTACTGAATAATCCAGCTA+AGG | + | chr4_1:68195471-68195490 | Msa0566960:CDS | 35.0% | |
| TGTAAGATTCAAACCTTAGC+TGG | - | chr4_1:68195487-68195506 | None:intergenic | 35.0% | |
| AAATAAAATACGCAACCGCG+TGG | - | chr4_1:68194667-68194686 | None:intergenic | 40.0% | |
| AGGGCCATATAAGTAACTTG+CGG | + | chr4_1:68194694-68194713 | Msa0566960:five_prime_UTR | 40.0% | |
| ! | GTAACTTGCGGAATTGTTAG+GGG | + | chr4_1:68194706-68194725 | Msa0566960:five_prime_UTR | 40.0% |
| ! | TAACTTGCGGAATTGTTAGG+GGG | + | chr4_1:68194707-68194726 | Msa0566960:five_prime_UTR | 40.0% |
| TAATGAGGGAAGTTACTCAC+AGG | + | chr4_1:68195015-68195034 | Msa0566960:exon | 40.0% | |
| CCCAACATTCTCAACAACAA+AGG | + | chr4_1:68195231-68195250 | Msa0566960:CDS | 40.0% | |
| GCGAATGCATGATAGATCAA+TGG | - | chr4_1:68195395-68195414 | None:intergenic | 40.0% | |
| AAATAAAATACGCAACCGCG+TGG | - | chr4_1:68194667-68194686 | None:intergenic | 40.0% | |
| AGGGCCATATAAGTAACTTG+CGG | + | chr4_1:68194694-68194713 | Msa0566960:five_prime_UTR | 40.0% | |
| ! | GTAACTTGCGGAATTGTTAG+GGG | + | chr4_1:68194706-68194725 | Msa0566960:five_prime_UTR | 40.0% |
| ! | TAACTTGCGGAATTGTTAGG+GGG | + | chr4_1:68194707-68194726 | Msa0566960:five_prime_UTR | 40.0% |
| TAATGAGGGAAGTTACTCAC+AGG | + | chr4_1:68195015-68195034 | Msa0566960:exon | 40.0% | |
| CCCAACATTCTCAACAACAA+AGG | + | chr4_1:68195231-68195250 | Msa0566960:CDS | 40.0% | |
| GCGAATGCATGATAGATCAA+TGG | - | chr4_1:68195395-68195414 | None:intergenic | 40.0% | |
| ATGGAAGGAAGCAAGCTAGA+TGG | - | chr4_1:68195376-68195395 | None:intergenic | 45.0% | |
| ATCTATCATGCATTCGCGGT+AGG | + | chr4_1:68195398-68195417 | Msa0566960:CDS | 45.0% | |
| TCTATCATGCATTCGCGGTA+GGG | + | chr4_1:68195399-68195418 | Msa0566960:CDS | 45.0% | |
| ATGGAAGGAAGCAAGCTAGA+TGG | - | chr4_1:68195376-68195395 | None:intergenic | 45.0% | |
| ATCTATCATGCATTCGCGGT+AGG | + | chr4_1:68195398-68195417 | Msa0566960:CDS | 45.0% | |
| TCTATCATGCATTCGCGGTA+GGG | + | chr4_1:68195399-68195418 | Msa0566960:CDS | 45.0% | |
| GGAAAGCAGTAGCTAGCACA+AGG | - | chr4_1:68194800-68194819 | None:intergenic | 50.0% | |
| GGAAAGCAGTAGCTAGCACA+AGG | - | chr4_1:68194800-68194819 | None:intergenic | 50.0% | |
| GCATTCGCGGTAGGGATGCG+TGG | + | chr4_1:68195407-68195426 | Msa0566960:CDS | 65.0% | |
| GCATTCGCGGTAGGGATGCG+TGG | + | chr4_1:68195407-68195426 | Msa0566960:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 68194657 | 68195507 | 68194657 | ID=Msa0566960;Name=Msa0566960 |
| chr4_1 | mRNA | 68194657 | 68195507 | 68194657 | ID=Msa0566960-mRNA-1;Parent=Msa0566960;Name=Msa0566960-mRNA-1;_AED=0.42;_eAED=0.42;_QI=360|1|1|1|1|1|3|9|88 |
| chr4_1 | exon | 68194657 | 68195036 | 68194657 | ID=Msa0566960-mRNA-1:exon:22506;Parent=Msa0566960-mRNA-1 |
| chr4_1 | exon | 68195141 | 68195252 | 68195141 | ID=Msa0566960-mRNA-1:exon:22507;Parent=Msa0566960-mRNA-1 |
| chr4_1 | exon | 68195364 | 68195507 | 68195364 | ID=Msa0566960-mRNA-1:exon:22508;Parent=Msa0566960-mRNA-1 |
| chr4_1 | five_prime_UTR | 68194657 | 68195016 | 68194657 | ID=Msa0566960-mRNA-1:five_prime_utr;Parent=Msa0566960-mRNA-1 |
| chr4_1 | CDS | 68195017 | 68195036 | 68195017 | ID=Msa0566960-mRNA-1:cds;Parent=Msa0566960-mRNA-1 |
| chr4_1 | CDS | 68195141 | 68195252 | 68195141 | ID=Msa0566960-mRNA-1:cds;Parent=Msa0566960-mRNA-1 |
| chr4_1 | CDS | 68195364 | 68195498 | 68195364 | ID=Msa0566960-mRNA-1:cds;Parent=Msa0566960-mRNA-1 |
| chr4_1 | three_prime_UTR | 68195499 | 68195507 | 68195499 | ID=Msa0566960-mRNA-1:three_prime_utr;Parent=Msa0566960-mRNA-1 |
| Gene Sequence |
| Protein sequence |
