AlfalfaGEDB | Msa0611800

M. sativa cultivar zhongmu-4 / Msa0611800

KEGG: -
GO: -
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0611800	XP_039685898.1	86.076	79	11	0	1	79	345	423	6.16e-38	142

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0611800	sp\|Q39202\|RLK1_ARATH	47.436	78	32	1	1	78	678	746	1.79e-19	84.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003890	Msa0611800	0.808642	2.801233e-50	-8.615850e-47
Msa0011170	Msa0611800	0.812691	3.732552e-51	-8.615850e-47
Msa0014710	Msa0611800	0.836704	8.004295e-57	-8.615850e-47
Msa0017510	Msa0611800	0.809442	1.888859e-50	-8.615850e-47
Msa0036140	Msa0611800	0.815412	9.368704e-52	-8.615850e-47
Msa0084380	Msa0611800	0.806519	7.906859e-50	-8.615850e-47
Msa0095560	Msa0611800	0.808660	2.776330e-50	-8.615850e-47
Msa0112750	Msa0611800	0.809247	2.079167e-50	-8.615850e-47
Msa0116770	Msa0611800	0.828847	7.160930e-55	-8.615850e-47
Msa0117770	Msa0611800	0.800514	1.390405e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0611800	MtrunA17_Chr1g0205561	86.076	79	11	0	1	79	345	423	6.84e-42	142
Msa0611800	MtrunA17_Chr5g0399591	57.143	77	29	2	1	77	655	727	2.93e-22	89.0
Msa0611800	MtrunA17_Chr5g0399561	55.844	77	30	2	1	77	658	730	7.53e-22	87.8
Msa0611800	MtrunA17_Chr2g0311511	48.718	78	35	2	1	78	590	662	1.06e-19	81.6
Msa0611800	MtrunA17_Chr2g0311521	59.649	57	23	0	1	57	887	943	2.11e-19	80.9
Msa0611800	MtrunA17_Chr4g0063701	56.452	62	27	0	1	62	673	734	5.48e-19	79.7
Msa0611800	MtrunA17_Chr2g0311541	57.895	57	24	0	1	57	644	700	5.58e-19	79.7
Msa0611800	MtrunA17_Chr2g0311551	56.140	57	25	0	1	57	881	937	1.63e-18	78.2
Msa0611800	MtrunA17_Chr8g0338471	58.929	56	23	0	1	56	668	723	2.15e-17	75.1
Msa0611800	MtrunA17_Chr8g0338501	58.929	56	23	0	1	56	699	754	2.31e-17	75.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0611800	AT2G19130.1	52.381	63	28	1	1	63	640	700	1.78e-14	67.0
Msa0611800	AT5G24080.3	48.276	58	30	0	1	58	550	607	2.39e-14	66.6
Msa0611800	AT5G24080.1	48.276	58	30	0	1	58	550	607	2.39e-14	66.6
Msa0611800	AT5G24080.2	48.276	58	30	0	1	58	677	734	2.43e-14	66.6
Msa0611800	AT4G00340.1	51.786	56	27	0	1	56	627	682	8.36e-14	65.1
Msa0611800	AT4G00340.2	51.786	56	27	0	1	56	606	661	8.69e-14	65.1
Msa0611800	AT1G34300.1	48.438	64	32	1	1	63	629	692	1.42e-13	64.7
Msa0611800	AT5G20050.1	50.000	56	28	0	1	56	257	312	5.71e-13	62.8
Msa0611800	AT2G23200.1	45.614	57	31	0	1	57	634	690	2.75e-12	60.8
Msa0611800	AT5G35370.1	42.857	63	36	0	1	63	660	722	3.04e-12	60.8

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Find 71 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTGGATATCTACAGCTT+TGG	0.498218	4_2:-60198535	Msa0611800:CDS
AAAGTTTCTATAGATAAAGA+AGG	0.504229	4_2:+60199594	None:intergenic
AAGGTGTTGCAGATGTCGGA+AGG	0.614948	4_2:-60198263	Msa0611800:three_prime_UTR
AAGTTGTTAATGATTTCTTT+AGG	0.353115	4_2:-60198355	Msa0611800:three_prime_UTR
AATGCCAGGTATCTCATAGA+AGG	0.436096	4_2:+60199401	None:intergenic
AATGTGTATTAGAATTGCTT+GGG	0.441866	4_2:-60199853	Msa0611800:intron
ACAAAATAGAGAGCAAGAAT+TGG	0.432074	4_2:+60198191	None:intergenic
ACAAATGTTAGAGGGACAAT+GGG	0.521045	4_2:-60198601	Msa0611800:CDS
ACTAATGTTTCTTTCATGAT+TGG	0.333872	4_2:-60200207	None:intergenic
ACTTTCCACTCACCCGTTAA+TGG	0.208512	4_2:+60199992	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAACATAGTCAACTTCTAAT+GGG	-	chr4_2:60197976-60197995	Msa0611800:three_prime_UTR	30.0%
CTCACTTATCTTATTCTTTC+AGG	-	chr4_2:60198078-60198097	Msa0611800:three_prime_UTR	30.0%
TTATCTTATTCTTTCAGGGA+AGG	-	chr4_2:60198083-60198102	Msa0611800:three_prime_UTR	30.0%
TATCTTATTCTTTCAGGGAA+GGG	-	chr4_2:60198084-60198103	Msa0611800:three_prime_UTR	30.0%
TAGAGCTGAAAAAAGAGAAT+GGG	+	chr4_2:60198244-60198263	None:intergenic	30.0%
ATAGAGCTGAAAAAAGAGAA+TGG	+	chr4_2:60198245-60198264	None:intergenic	30.0%
ATGAAGCCAGGAAAATTAAA+AGG	+	chr4_2:60198285-60198304	None:intergenic	30.0%
ATACACATTATAATGAAGCC+AGG	+	chr4_2:60198297-60198316	None:intergenic	30.0%
TATGTCCATCTAACTCAAAA+TGG	+	chr4_2:60198519-60198538	None:intergenic	30.0%
CTTCATACTAAGAAAGAAGA+AGG	-	chr4_2:60198670-60198689	Msa0611800:five_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	60197961	60200222	60197961	ID=Msa0611800;Name=Msa0611800
chr4_2	mRNA	60197961	60200222	60197961	ID=Msa0611800-mRNA-1;Parent=Msa0611800;Name=Msa0611800-mRNA-1;_AED=0.01;_eAED=0.01;_QI=756\|1\|1\|1\|0.5\|0.4\|5\|441\|86
chr4_2	exon	60197961	60198776	60197961	ID=Msa0611800-mRNA-1:exon:16124;Parent=Msa0611800-mRNA-1
chr4_2	exon	60199326	60199710	60199326	ID=Msa0611800-mRNA-1:exon:16123;Parent=Msa0611800-mRNA-1
chr4_2	exon	60199854	60199924	60199854	ID=Msa0611800-mRNA-1:exon:16122;Parent=Msa0611800-mRNA-1
chr4_2	exon	60200005	60200083	60200005	ID=Msa0611800-mRNA-1:exon:16121;Parent=Msa0611800-mRNA-1
chr4_2	exon	60200116	60200222	60200116	ID=Msa0611800-mRNA-1:exon:16120;Parent=Msa0611800-mRNA-1
chr4_2	five_prime_UTR	60200116	60200222	60200116	ID=Msa0611800-mRNA-1:five_prime_utr;Parent=Msa0611800-mRNA-1
chr4_2	five_prime_UTR	60200005	60200083	60200005	ID=Msa0611800-mRNA-1:five_prime_utr;Parent=Msa0611800-mRNA-1
chr4_2	five_prime_UTR	60199854	60199924	60199854	ID=Msa0611800-mRNA-1:five_prime_utr;Parent=Msa0611800-mRNA-1
chr4_2	five_prime_UTR	60199326	60199710	60199326	ID=Msa0611800-mRNA-1:five_prime_utr;Parent=Msa0611800-mRNA-1
chr4_2	five_prime_UTR	60198663	60198776	60198663	ID=Msa0611800-mRNA-1:five_prime_utr;Parent=Msa0611800-mRNA-1
chr4_2	CDS	60198402	60198662	60198402	ID=Msa0611800-mRNA-1:cds;Parent=Msa0611800-mRNA-1
chr4_2	three_prime_UTR	60197961	60198401	60197961	ID=Msa0611800-mRNA-1:three_prime_utr;Parent=Msa0611800-mRNA-1

Gene Sequence	>Msa0611800 ATGGCCAAGCTGCTAATGAAAGACAGAAGTAGAACAAGCACAAATGTTAGAGGGACAATGGGATGTTTGGCACCAGAATGGCTCAAAAATGCACCTATCACTGCAAAAGTGGATATCTACAGCTTTGGTGTCATGTTGCTTGAAATCTTATTTTGTAGAAGGCACATTGAGTTGCATCAAGTTGAAAATGGAACAGAAGGTAGTGATGACATGATACTTATAGATTTTGGGTTCTATATTGAGCAAAAGAGGGGAAACTAA
Protein sequence	>Msa0611800 MAKLLMKDRSRTSTNVRGTMGCLAPEWLKNAPITAKVDIYSFGVMLLEILFCRRHIELHQVENGTEGSDDMILIDFGFYIEQKRGN*