AlfalfaGEDB | Msa0420000

M. sativa cultivar zhongmu-4 / Msa0420000

KEGG: K21374
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0008194 GO:0015020 GO:0016740 GO:0016757 GO:0016758 GO:0035251 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046527 GO:0050403 GO:0050502 GO:0080043 GO:0080044
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0420000	KEH35119.1	88.462	78	9	0	8	85	411	488	1.25e-41	152

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0420000	sp\|Q6VAB3\|U85A8_STERE	53.947	76	35	0	8	83	404	479	2.39e-22	92.0

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0420000	A0A072UZ84	88.462	78	9	0	8	85	411	488	5.96e-42	152

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000490	Msa0420000	-0.842934	1.909463e-58	-8.615850e-47
Msa0024680	Msa0420000	0.814106	1.824033e-51	-8.615850e-47
Msa0030310	Msa0420000	0.811824	5.771549e-51	-8.615850e-47
Msa0032370	Msa0420000	0.815244	1.020756e-51	-8.615850e-47
Msa0094920	Msa0420000	0.811665	6.248375e-51	-8.615850e-47
Msa0095010	Msa0420000	0.818187	2.234257e-52	-8.615850e-47
Msa0095380	Msa0420000	0.830462	2.897311e-55	-8.615850e-47
Msa0113160	Msa0420000	0.814447	1.533631e-51	-8.615850e-47
Msa0137040	Msa0420000	0.824554	7.580261e-54	-8.615850e-47
Msa0143080	Msa0420000	0.832996	6.872068e-56	-8.615850e-47
Msa0145950	Msa0420000	0.837578	4.785761e-57	-8.615850e-47
Msa0168640	Msa0420000	-0.802249	6.135715e-49	-8.615850e-47
Msa0169620	Msa0420000	0.831761	1.389584e-55	-8.615850e-47
Msa0172350	Msa0420000	0.824422	8.142110e-54	-8.615850e-47
Msa0173740	Msa0420000	0.808477	3.038932e-50	-8.615850e-47
Msa0173820	Msa0420000	0.805124	1.553238e-49	-8.615850e-47
Msa0178990	Msa0420000	0.807407	5.132709e-50	-8.615850e-47
Msa0183620	Msa0420000	0.800314	1.527840e-48	-8.615850e-47
Msa0188680	Msa0420000	-0.818795	1.626260e-52	-8.615850e-47
Msa0194970	Msa0420000	0.807899	4.034724e-50	-8.615850e-47
Msa0199690	Msa0420000	0.802894	4.517057e-49	-8.615850e-47
Msa0223100	Msa0420000	0.809788	1.591669e-50	-8.615850e-47
Msa0223290	Msa0420000	0.843433	1.405290e-58	-8.615850e-47
Msa0223380	Msa0420000	0.823583	1.281185e-53	-8.615850e-47
Msa0236980	Msa0420000	0.846724	1.814887e-59	-8.615850e-47
Msa0377530	Msa0420000	0.834893	2.302487e-56	-8.615850e-47
Msa0380040	Msa0420000	0.804343	2.261304e-49	-8.615850e-47
Msa0393950	Msa0420000	0.831373	1.732098e-55	-8.615850e-47
Msa0398640	Msa0420000	0.800065	1.716425e-48	-8.615850e-47
Msa0419820	Msa0420000	0.808341	3.248797e-50	-8.615850e-47
Msa0420000	Msa0426810	0.815690	8.122516e-52	-8.615850e-47
Msa0420000	Msa0429290	0.807988	3.862813e-50	-8.615850e-47
Msa0420000	Msa0436280	-0.816639	4.985542e-52	-8.615850e-47
Msa0420000	Msa0436460	0.813296	2.749634e-51	-8.615850e-47
Msa0420000	Msa0441800	0.817362	3.430243e-52	-8.615850e-47
Msa0420000	Msa0460930	0.815056	1.124245e-51	-8.615850e-47
Msa0420000	Msa0465920	0.824076	9.820139e-54	-8.615850e-47
Msa0420000	Msa0465960	0.810504	1.115389e-50	-8.615850e-47
Msa0420000	Msa0468160	0.800598	1.336657e-48	-8.615850e-47
Msa0420000	Msa0469910	-0.834602	2.725628e-56	-8.615850e-47
Msa0420000	Msa0473240	0.811485	6.839530e-51	-8.615850e-47
Msa0420000	Msa0476320	0.804189	2.433698e-49	-8.615850e-47
Msa0420000	Msa0480320	0.818856	1.575842e-52	-8.615850e-47
Msa0420000	Msa0487910	0.801440	8.996058e-49	-8.615850e-47
Msa0420000	Msa0490300	0.808166	3.539480e-50	-8.615850e-47
Msa0420000	Msa0506940	0.801012	1.100621e-48	-8.615850e-47
Msa0420000	Msa0508200	0.807164	5.778899e-50	-8.615850e-47
Msa0420000	Msa0517490	0.825770	3.910446e-54	-8.615850e-47
Msa0420000	Msa0520320	0.815715	8.020866e-52	-8.615850e-47
Msa0420000	Msa0521880	0.815014	1.148218e-51	-8.615850e-47
Msa0420000	Msa0525340	0.817060	4.010401e-52	-8.615850e-47
Msa0420000	Msa0526710	0.816044	6.773666e-52	-8.615850e-47
Msa0420000	Msa0526890	0.812387	4.349777e-51	-8.615850e-47
Msa0420000	Msa0527590	0.817477	3.231508e-52	-8.615850e-47
Msa0420000	Msa0535030	0.843886	1.063626e-58	-8.615850e-47
Msa0420000	Msa0535060	0.841261	5.290601e-58	-8.615850e-47
Msa0420000	Msa0542160	0.805111	1.563249e-49	-8.615850e-47
Msa0420000	Msa0575990	-0.811575	6.537051e-51	-8.615850e-47
Msa0420000	Msa0576100	-0.818801	1.621191e-52	-8.615850e-47
Msa0420000	Msa0576980	0.802251	6.129931e-49	-8.615850e-47
Msa0420000	Msa0581920	0.811192	7.917880e-51	-8.615850e-47
Msa0420000	Msa0590680	0.805842	1.098202e-49	-8.615850e-47
Msa0420000	Msa0591060	0.827824	1.263828e-54	-8.615850e-47
Msa0420000	Msa0591770	0.847814	9.111726e-60	-8.615850e-47
Msa0420000	Msa0598460	0.814836	1.257550e-51	-8.615850e-47
Msa0420000	Msa0607200	0.803872	2.833555e-49	-8.615850e-47
Msa0420000	Msa0618010	0.818912	1.529822e-52	-8.615850e-47
Msa0420000	Msa0625280	0.810076	1.379978e-50	-8.615850e-47
Msa0420000	Msa0638280	0.820805	5.650124e-53	-8.615850e-47
Msa0420000	Msa0638320	0.825429	4.711336e-54	-8.615850e-47
Msa0420000	Msa0642650	0.801343	9.414506e-49	-8.615850e-47
Msa0420000	Msa0647380	0.802072	6.671875e-49	-8.615850e-47
Msa0420000	Msa0663900	0.806455	8.157118e-50	-8.615850e-47
Msa0420000	Msa0669850	0.847619	1.031485e-59	-8.615850e-47
Msa0420000	Msa0670270	0.817659	2.940917e-52	-8.615850e-47
Msa0420000	Msa0677270	0.827775	1.298517e-54	-8.615850e-47
Msa0420000	Msa0707390	0.805884	1.076233e-49	-8.615850e-47
Msa0420000	Msa0711490	0.813074	3.077491e-51	-8.615850e-47
Msa0420000	Msa0712310	0.832110	1.139425e-55	-8.615850e-47
Msa0420000	Msa0743680	0.831309	1.795707e-55	-8.615850e-47
Msa0420000	Msa0789270	0.812994	3.204113e-51	-8.615850e-47
Msa0420000	Msa0789280	0.807932	3.970388e-50	-8.615850e-47
Msa0420000	Msa0789290	0.805461	1.320373e-49	-8.615850e-47
Msa0420000	Msa0789300	0.801741	7.805351e-49	-8.615850e-47
Msa0420000	Msa0790390	-0.827906	1.207826e-54	-8.615850e-47
Msa0420000	Msa0792090	0.832532	8.959555e-56	-8.615850e-47
Msa0420000	Msa0795010	0.819592	1.071309e-52	-8.615850e-47
Msa0420000	Msa0795030	0.834623	2.693163e-56	-8.615850e-47
Msa0420000	Msa0824730	0.800209	1.604748e-48	-8.615850e-47
Msa0420000	Msa0830990	0.832809	7.645982e-56	-8.615850e-47
Msa0420000	Msa0850960	0.821181	4.628794e-53	-8.615850e-47
Msa0420000	Msa0852730	0.808810	2.579097e-50	-8.615850e-47
Msa0420000	Msa0863800	0.809447	1.883932e-50	-8.615850e-47
Msa0420000	Msa0874500	0.812857	3.433321e-51	-8.615850e-47
Msa0420000	Msa0890250	0.803359	3.619018e-49	-8.615850e-47
Msa0420000	Msa0903330	0.816513	5.319212e-52	-8.615850e-47
Msa0420000	Msa0905260	0.816226	6.169282e-52	-8.615850e-47
Msa0420000	Msa0908170	0.801404	9.148453e-49	-8.615850e-47
Msa0420000	Msa0933440	0.804809	1.807612e-49	-8.615850e-47
Msa0420000	Msa0939790	0.812219	4.733272e-51	-8.615850e-47
Msa0420000	Msa0946080	0.805620	1.222263e-49	-8.615850e-47
Msa0420000	Msa0959010	0.834713	2.556295e-56	-8.615850e-47
Msa0420000	Msa0974530	0.815422	9.320893e-52	-8.615850e-47
Msa0420000	Msa0974810	0.814257	1.689087e-51	-8.615850e-47
Msa0420000	Msa0993530	0.820824	5.592454e-53	-8.615850e-47
Msa0420000	Msa1000940	0.812117	4.982819e-51	-8.615850e-47
Msa0420000	Msa1015060	0.823412	1.404371e-53	-8.615850e-47
Msa0420000	Msa1026760	0.808570	2.902388e-50	-8.615850e-47
Msa0420000	Msa1026790	0.811302	7.491951e-51	-8.615850e-47
Msa0420000	Msa1044340	0.816589	5.116248e-52	-8.615850e-47
Msa0420000	Msa1047400	0.802342	5.872592e-49	-8.615850e-47
Msa0420000	Msa1048330	0.807390	5.174393e-50	-8.615850e-47
Msa0420000	Msa1069660	-0.806752	7.062016e-50	-8.615850e-47
Msa0420000	Msa1089840	0.802936	4.428669e-49	-8.615850e-47
Msa0420000	Msa1092820	0.810834	9.464213e-51	-8.615850e-47
Msa0420000	Msa1111700	0.827569	1.455343e-54	-8.615850e-47
Msa0420000	Msa1127830	0.816293	5.959831e-52	-8.615850e-47
Msa0420000	Msa1130210	-0.832549	8.875130e-56	-8.615850e-47
Msa0420000	Msa1171770	-0.816140	6.446723e-52	-8.615850e-47
Msa0420000	Msa1193950	-0.808154	3.560763e-50	-8.615850e-47
Msa0420000	Msa1217860	0.820433	6.878609e-53	-8.615850e-47
Msa0420000	Msa1219110	0.818361	2.040409e-52	-8.615850e-47
Msa0420000	Msa1237030	0.822368	2.459392e-53	-8.615850e-47
Msa0420000	Msa1246530	-0.809304	2.021904e-50	-8.615850e-47
Msa0420000	Msa1269300	0.803963	2.712171e-49	-8.615850e-47
Msa0420000	Msa1269580	-0.820762	5.780160e-53	-8.615850e-47
Msa0420000	Msa1279920	0.808333	3.260515e-50	-8.615850e-47
Msa0420000	Msa1280130	0.803227	3.855758e-49	-8.615850e-47
Msa0420000	Msa1291350	0.804650	1.951386e-49	-8.615850e-47
Msa0420000	Msa1362510	0.831269	1.836761e-55	-8.615850e-47
Msa0420000	Msa1365150	0.808662	2.774564e-50	-8.615850e-47
Msa0420000	Msa1365200	0.806275	8.901303e-50	-8.615850e-47
Msa0420000	Msa1379990	0.811776	5.912047e-51	-8.615850e-47
Msa0420000	Msa1382310	0.806020	1.007654e-49	-8.615850e-47
Msa0420000	Msa1390310	0.826512	2.604647e-54	-8.615850e-47
Msa0420000	Msa1403580	-0.841125	5.741950e-58	-8.615850e-47
Msa0420000	Msa1403930	-0.815883	7.356191e-52	-8.615850e-47
Msa0420000	Msa1412140	0.802314	5.949926e-49	-8.615850e-47
Msa0420000	Msa1429360	-0.813948	1.976023e-51	-8.615850e-47
Msa0420000	Msa1438640	0.805516	1.285688e-49	-8.615850e-47
Msa0420000	Msa1439960	0.828562	8.388803e-55	-8.615850e-47
Msa0420000	Msa1442220	-0.807951	3.932480e-50	-8.615850e-47
Msa0420000	Msa1443290	-0.832592	8.656008e-56	-8.615850e-47
Msa0420000	Msa1452470	0.820386	7.051867e-53	-8.615850e-47
Msa0420000	Msa1454110	0.805951	1.041636e-49	-8.615850e-47
Msa0420000	Msa1456650	0.814712	1.339867e-51	-8.615850e-47
Msa0251250	Msa0420000	0.816018	6.864103e-52	-8.615850e-47
Msa0260210	Msa0420000	0.809149	2.182183e-50	-8.615850e-47
Msa0267760	Msa0420000	0.815692	8.114227e-52	-8.615850e-47
Msa0270220	Msa0420000	-0.826017	3.417373e-54	-8.615850e-47
Msa0275030	Msa0420000	0.801588	8.388118e-49	-8.615850e-47
Msa0275400	Msa0420000	-0.809662	1.693796e-50	-8.615850e-47
Msa0280490	Msa0420000	0.836858	7.312057e-57	-8.615850e-47
Msa0288240	Msa0420000	0.801651	8.140905e-49	-8.615850e-47
Msa0302650	Msa0420000	0.830646	2.612288e-55	-8.615850e-47
Msa0304290	Msa0420000	0.818405	1.993722e-52	-8.615850e-47
Msa0304330	Msa0420000	0.819312	1.240645e-52	-8.615850e-47
Msa0305890	Msa0420000	0.805200	1.497525e-49	-8.615850e-47
Msa0307830	Msa0420000	-0.830031	3.691684e-55	-8.615850e-47
Msa0314120	Msa0420000	0.800376	1.483718e-48	-8.615850e-47
Msa0315240	Msa0420000	0.806900	6.572193e-50	-8.615850e-47
Msa0320100	Msa0420000	0.806823	6.822299e-50	-8.615850e-47
Msa0326670	Msa0420000	0.813162	2.942363e-51	-8.615850e-47
Msa0328210	Msa0420000	0.841647	4.185422e-58	-8.615850e-47
Msa0340810	Msa0420000	0.805275	1.443904e-49	-8.615850e-47
Msa0344640	Msa0420000	0.802760	4.814813e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0420000	MtrunA17_Chr3g0118691	88.462	78	9	0	8	85	411	488	1.34e-45	152
Msa0420000	MtrunA17_Chr3g0118721	78.313	83	18	0	3	85	213	295	2.07e-40	134
Msa0420000	MtrunA17_Chr3g0118701	78.205	78	17	0	8	85	411	488	2.80e-39	135
Msa0420000	MtrunA17_Chr3g0118711	77.922	77	17	0	8	84	409	485	4.59e-39	134
Msa0420000	MtrunA17_Chr3g0118751	74.359	78	20	0	8	85	373	450	2.14e-36	127
Msa0420000	MtrunA17_Chr3g0118771	74.359	78	20	0	8	85	417	494	5.95e-36	126
Msa0420000	MtrunA17_Chr3g0118671	64.935	77	27	0	8	84	402	478	7.23e-33	117
Msa0420000	MtrunA17_Chr6g0454351	54.667	75	34	0	6	80	391	465	6.93e-26	98.6
Msa0420000	MtrunA17_Chr6g0454211	54.667	75	34	0	6	80	693	767	8.80e-24	93.2
Msa0420000	MtrunA17_Chr6g0454331	54.667	75	34	0	6	80	405	479	1.23e-23	92.4
Msa0420000	MtrunA17_Chr6g0454121	51.282	78	38	0	3	80	402	479	2.75e-23	91.7
Msa0420000	MtrunA17_Chr6g0454401	52.564	78	37	0	3	80	404	481	3.15e-23	91.3
Msa0420000	MtrunA17_Chr6g0454201	52.000	75	36	0	6	80	407	481	8.22e-23	90.1
Msa0420000	MtrunA17_Chr5g0423981	51.282	78	38	0	3	80	368	445	9.40e-23	90.1
Msa0420000	MtrunA17_Chr6g0454261	53.333	75	35	0	6	80	406	480	1.03e-22	90.1
Msa0420000	MtrunA17_Chr6g0454191	51.282	78	38	0	3	80	404	481	1.28e-22	89.7
Msa0420000	MtrunA17_Chr6g0454291	50.667	75	37	0	6	80	403	477	1.57e-22	89.4
Msa0420000	MtrunA17_Chr7g0217881	49.383	81	41	0	3	83	385	465	1.85e-22	89.4
Msa0420000	MtrunA17_Chr8g0336331	52.000	75	36	0	6	80	403	477	4.96e-22	88.2
Msa0420000	MtrunA17_Chr6g0453961	51.282	78	38	0	3	80	413	490	5.35e-22	88.2
Msa0420000	MtrunA17_Chr6g0454131	48.101	79	41	0	7	85	406	484	6.53e-22	87.8
Msa0420000	MtrunA17_Chr6g0452771	50.667	75	37	0	6	80	406	480	7.06e-22	87.8
Msa0420000	MtrunA17_Chr6g0453951	48.000	75	39	0	6	80	453	527	1.02e-21	87.4
Msa0420000	MtrunA17_Chr3g0078841	48.000	75	39	0	6	80	404	478	1.17e-21	87.0
Msa0420000	MtrunA17_Chr5g0427561	50.000	78	39	0	6	83	11	88	1.32e-21	80.5
Msa0420000	MtrunA17_Chr2g0278711	50.000	78	38	1	8	84	404	481	1.34e-21	87.0
Msa0420000	MtrunA17_Chr8g0359821	50.667	75	37	0	6	80	404	478	3.66e-21	85.5
Msa0420000	MtrunA17_Chr6g0454341	48.000	75	39	0	6	80	404	478	3.68e-21	85.5
Msa0420000	MtrunA17_Chr6g0454271	50.667	75	37	0	6	80	213	287	4.14e-21	84.0
Msa0420000	MtrunA17_Chr6g0454301	51.389	72	35	0	7	78	157	228	5.30e-21	85.1
Msa0420000	MtrunA17_Chr6g0454301	50.667	75	37	0	6	80	657	731	8.45e-21	84.7
Msa0420000	MtrunA17_Chr6g0454361	48.000	75	39	0	6	80	407	481	1.77e-20	83.6
Msa0420000	MtrunA17_Chr6g0454221	48.101	79	41	0	3	81	154	232	1.84e-20	82.4
Msa0420000	MtrunA17_Chr6g0454151	48.000	75	39	0	6	80	404	478	2.23e-20	83.6
Msa0420000	MtrunA17_Chr4g0050341	52.174	69	33	0	8	76	419	487	4.63e-20	82.4
Msa0420000	MtrunA17_Chr6g0454171	46.154	78	42	0	6	83	236	313	9.08e-20	80.9
Msa0420000	MtrunA17_Chr8g0355011	48.000	75	39	0	6	80	288	362	9.19e-20	81.3
Msa0420000	MtrunA17_Chr6g0454141	46.667	75	40	0	6	80	406	480	2.20e-19	80.5
Msa0420000	MtrunA17_Chr6g0454181	43.590	78	44	0	3	80	403	480	4.66e-18	77.0
Msa0420000	MtrunA17_Chr5g0426431	51.351	74	34	2	8	80	406	478	5.49e-18	76.6
Msa0420000	MtrunA17_Chr6g0454371	48.000	75	39	0	6	80	97	171	1.38e-17	72.8
Msa0420000	MtrunA17_Chr3g0118661	51.282	78	38	0	8	85	408	485	1.41e-17	75.5
Msa0420000	MtrunA17_Chr6g0454321	49.153	59	30	0	6	64	404	462	1.69e-17	75.5
Msa0420000	MtrunA17_Chr3g0092641	47.297	74	37	2	8	80	406	478	3.45e-17	74.3
Msa0420000	MtrunA17_Chr6g0454231	47.368	76	39	1	6	80	144	219	9.24e-17	73.2
Msa0420000	MtrunA17_Chr5g0426401	48.649	74	36	2	8	80	239	311	6.68e-14	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0420000	AT1G22380.1	47.297	74	38	1	8	80	408	481	4.55e-19	80.1
Msa0420000	AT1G22400.1	44.595	74	40	1	8	80	409	482	2.98e-18	77.8
Msa0420000	AT1G22340.1	43.421	76	42	1	6	80	407	482	8.15e-18	76.6
Msa0420000	AT1G78270.1	43.243	74	41	1	8	80	408	481	7.43e-16	70.9
Msa0420000	AT1G22360.1	43.243	74	41	1	8	80	405	478	6.66e-15	68.2
Msa0420000	AT1G22370.2	50.000	74	36	1	8	80	403	476	4.17e-14	65.9
Msa0420000	AT1G22370.1	50.000	74	36	1	8	80	233	306	9.46e-14	64.7

Find 21 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTAACCATGATGTTAAA+AGG	0.220501	3_2:-68272508	Msa0420000:CDS
TTATGCAAGTCTTGGAAAAT+AGG	0.270182	3_2:-68272536	Msa0420000:CDS
CATAAACACCTACAATTTGC+AGG	0.318665	3_2:+68272554	None:intergenic
TATGCAAGTCTTGGAAAATA+GGG	0.395953	3_2:-68272535	Msa0420000:CDS
CTAGAAAAGCTACTAATTTG+AGG	0.420613	3_2:-68272397	Msa0420000:CDS
GCTACGCCCCTGCAAATTGT+AGG	0.460125	3_2:-68272562	Msa0420000:CDS
AATTAACCATGATGTTAAAA+GGG	0.468721	3_2:-68272507	Msa0420000:CDS
GTAGGTGTTTATGCAAGTCT+TGG	0.469753	3_2:-68272544	Msa0420000:CDS
AGAAATTGTGATGGAAATGA+TGG	0.474306	3_2:-68272474	Msa0420000:CDS
TAGAAAAGCTACTAATTTGA+GGG	0.476899	3_2:-68272396	Msa0420000:CDS
AGAAATGAGACAAAAGTGCT+TGG	0.515737	3_2:-68272435	Msa0420000:CDS
GGCTACCCCTTGGTCATGAA+GGG	0.558714	3_2:-68272584	None:intergenic
GGAAATGATGGAAGGAGACA+AGG	0.560854	3_2:-68272462	Msa0420000:CDS
TGATATAACAGAAATTGTGA+TGG	0.569075	3_2:-68272483	Msa0420000:CDS
ATAAACACCTACAATTTGCA+GGG	0.573449	3_2:+68272555	None:intergenic
CAAGTCTTGGAAAATAGGGA+TGG	0.583190	3_2:-68272531	Msa0420000:CDS
GAAATGATGGAAGGAGACAA+GGG	0.612695	3_2:-68272461	Msa0420000:CDS
TGAGACAAAAGTGCTTGGAG+TGG	0.660296	3_2:-68272430	Msa0420000:CDS
ATTGTGATGGAAATGATGGA+AGG	0.667644	3_2:-68272470	Msa0420000:CDS
AGAAAAGCTACTAATTTGAG+GGG	0.678503	3_2:-68272395	Msa0420000:CDS
TAAACACCTACAATTTGCAG+GGG	0.705203	3_2:+68272556	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATTAACCATGATGTTAAA+AGG	-	chr3_2:68272395-68272414	Msa0420000:CDS	20.0%
!!	AATTAACCATGATGTTAAAA+GGG	-	chr3_2:68272396-68272415	Msa0420000:CDS	20.0%
!	TGATATAACAGAAATTGTGA+TGG	-	chr3_2:68272420-68272439	Msa0420000:CDS	25.0%
!	TAGAAAAGCTACTAATTTGA+GGG	-	chr3_2:68272507-68272526	Msa0420000:CDS	25.0%
!!	TTTTCAGAAGTTAATCAAAG+AGG	-	chr3_2:68272546-68272565	Msa0420000:CDS	25.0%
	ATAAACACCTACAATTTGCA+GGG	+	chr3_2:68272351-68272370	None:intergenic	30.0%
	TTATGCAAGTCTTGGAAAAT+AGG	-	chr3_2:68272367-68272386	Msa0420000:CDS	30.0%
	TATGCAAGTCTTGGAAAATA+GGG	-	chr3_2:68272368-68272387	Msa0420000:CDS	30.0%
!	TATCATCCCTTTTAACATCA+TGG	+	chr3_2:68272405-68272424	None:intergenic	30.0%
!	AGAAATTGTGATGGAAATGA+TGG	-	chr3_2:68272429-68272448	Msa0420000:CDS	30.0%
	CTAGAAAAGCTACTAATTTG+AGG	-	chr3_2:68272506-68272525	Msa0420000:CDS	30.0%
	AGAAAAGCTACTAATTTGAG+GGG	-	chr3_2:68272508-68272527	Msa0420000:CDS	30.0%
	TAAACACCTACAATTTGCAG+GGG	+	chr3_2:68272350-68272369	None:intergenic	35.0%
	CATAAACACCTACAATTTGC+AGG	+	chr3_2:68272352-68272371	None:intergenic	35.0%
!	ATTGTGATGGAAATGATGGA+AGG	-	chr3_2:68272433-68272452	Msa0420000:CDS	35.0%
!!	AGAAATGAGACAAAAGTGCT+TGG	-	chr3_2:68272468-68272487	Msa0420000:CDS	35.0%
	GTAGGTGTTTATGCAAGTCT+TGG	-	chr3_2:68272359-68272378	Msa0420000:CDS	40.0%
	CAAGTCTTGGAAAATAGGGA+TGG	-	chr3_2:68272372-68272391	Msa0420000:CDS	40.0%
!	GAAATGATGGAAGGAGACAA+GGG	-	chr3_2:68272442-68272461	Msa0420000:CDS	40.0%
!	GGAAATGATGGAAGGAGACA+AGG	-	chr3_2:68272441-68272460	Msa0420000:CDS	45.0%
!!	TGAGACAAAAGTGCTTGGAG+TGG	-	chr3_2:68272473-68272492	Msa0420000:CDS	45.0%
	GCTACGCCCCTGCAAATTGT+AGG	-	chr3_2:68272341-68272360	Msa0420000:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	68272334	68272591	68272334	ID=Msa0420000;Name=Msa0420000
chr3_2	mRNA	68272334	68272591	68272334	ID=Msa0420000-mRNA-1;Parent=Msa0420000;Name=Msa0420000-mRNA-1;_AED=0.50;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|85
chr3_2	exon	68272334	68272591	68272334	ID=Msa0420000-mRNA-1:exon:16102;Parent=Msa0420000-mRNA-1
chr3_2	CDS	68272334	68272591	68272334	ID=Msa0420000-mRNA-1:cds;Parent=Msa0420000-mRNA-1

Gene Sequence	>Msa0420000 ATGAAGGGCTACGCCCCTGCAAATTGTAGGTGTTTATGCAAGTCTTGGAAAATAGGGATGGAAATTAACCATGATGTTAAAAGGGATGATATAACAGAAATTGTGATGGAAATGATGGAAGGAGACAAGGGAAAAGAAATGAGACAAAAGTGCTTGGAGTGGAAGAATAAAGCTAGAAAAGCTACTAATTTGAGGGGATCATCATACAATAATTTTCAGAAGTTAATCAAAGAGGTTCTTCATCATAAAGCTATTTGA
Protein sequence	>Msa0420000 MKGYAPANCRCLCKSWKIGMEINHDVKRDDITEIVMEMMEGDKGKEMRQKCLEWKNKARKATNLRGSSYNNFQKLIKEVLHHKAI*