AlfalfaGEDB | Msa0460930

M. sativa cultivar zhongmu-4 / Msa0460930

KEGG: K07766
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0016787 GO:0016817 GO:0016818 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
PFAM: PF00293.31

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0460930	XP_003600707.2	99.487	195	1	0	1	195	23	217	1.89e-138	395

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0460930	sp\|Q9LQU5\|NUD18_ARATH	68.987	158	47	1	10	167	1	156	2.54e-74	224

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0460930	A0A396IVT9	99.487	195	1	0	1	195	23	217	9.04e-139	395

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000490	Msa0460930	-0.818011	2.448147e-52	-8.615850e-47
Msa0003930	Msa0460930	0.813075	3.074409e-51	-8.615850e-47
Msa0010020	Msa0460930	0.805584	1.243947e-49	-8.615850e-47
Msa0018620	Msa0460930	0.806753	7.058117e-50	-8.615850e-47
Msa0032370	Msa0460930	0.823246	1.535675e-53	-8.615850e-47
Msa0044020	Msa0460930	0.812575	3.957408e-51	-8.615850e-47
Msa0048000	Msa0460930	0.815414	9.357930e-52	-8.615850e-47
Msa0055150	Msa0460930	0.809044	2.298847e-50	-8.615850e-47
Msa0057140	Msa0460930	0.801794	7.609831e-49	-8.615850e-47
Msa0068760	Msa0460930	0.813328	2.706371e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0460930	MtrunA17_Chr3g0110281	99.487	195	1	0	1	195	23	217	1.74e-142	395
Msa0460930	MtrunA17_Chr5g0434521	70.984	193	52	2	1	191	1	191	3.64e-96	277
Msa0460930	MtrunA17_Chr1g0161131	66.871	163	51	2	10	171	1	161	3.42e-74	221
Msa0460930	MtrunA17_Chr8g0391621	63.804	163	56	2	10	171	1	161	2.59e-71	213
Msa0460930	MtrunA17_Chr6g0479111	62.500	160	55	3	8	166	70	225	4.92e-67	205
Msa0460930	MtrunA17_Chr8g0353331	51.250	160	67	3	13	164	4	160	2.17e-48	155
Msa0460930	MtrunA17_Chr7g0252391	45.614	171	84	4	15	177	6	175	4.77e-39	133
Msa0460930	MtrunA17_Chr1g0189471	51.562	64	26	2	13	74	4	64	8.80e-15	66.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0460930	AT1G14860.1	68.987	158	47	1	10	167	1	156	2.58e-75	224
Msa0460930	AT2G01670.1	65.625	160	53	1	8	167	4	161	8.19e-74	221
Msa0460930	AT1G18300.1	64.151	159	55	2	8	165	38	195	2.77e-71	215
Msa0460930	AT1G73540.1	60.870	161	55	3	8	166	39	193	5.89e-65	199
Msa0460930	AT3G26690.1	46.629	178	81	5	12	177	3	178	5.76e-45	148
Msa0460930	AT3G26690.2	46.629	178	81	5	12	177	3	178	5.76e-45	148
Msa0460930	AT1G12880.1	47.879	165	73	4	12	164	3	166	2.43e-42	141
Msa0460930	AT3G12600.1	50.000	158	72	2	10	160	1	158	9.23e-40	134
Msa0460930	AT3G12600.2	50.000	146	66	2	22	160	4	149	5.58e-36	124

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Find 65 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCTTCGTCAAAGAAGA+TGG	0.452927	3_3:-58605094	Msa0460930:CDS
AAAACTTCATCTCCAGTAAA+GGG	0.456071	3_3:+58604090	None:intergenic
AAAACTTCTTGAAGTCAATT+AGG	0.266342	3_3:-58604289	Msa0460930:three_prime_UTR
AAACCTTCGTCAAAGAAGAT+GGG	0.587211	3_3:-58605093	Msa0460930:intron
AAAGGTCGTCGTCAAGTTGT+TGG	0.437350	3_3:-58606466	Msa0460930:CDS
AACCTCTCTAATATGAAGAA+GGG	0.605647	3_3:-58604183	Msa0460930:three_prime_UTR
AAGGTCGTCGTCAAGTTGTT+GGG	0.471306	3_3:-58606465	Msa0460930:intron
AATAATTTGGCTCAATTCAA+TGG	0.336424	3_3:+58604515	None:intergenic
AATTCCTCTTGAACAAGTAG+AGG	0.573287	3_3:+58605134	None:intergenic
ACACACAATAATGGTTGCTT+TGG	0.348822	3_3:-58606551	Msa0460930:exon

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CCTTACAAAACACACAATAA+TGG	-	chr3_3:58604095-58604114	Msa0460930:three_prime_UTR	30.0%
TCAAGAAAATGTTGTCACAT+TGG	-	chr3_3:58604131-58604150	Msa0460930:three_prime_UTR	30.0%
GAGTTACAACGTTATAGAAA+AGG	-	chr3_3:58604171-58604190	Msa0460930:three_prime_UTR	30.0%
AAATTGTGACTTTCATTGTG+GGG	-	chr3_3:58604292-58604311	Msa0460930:three_prime_UTR	30.0%
CACCTCAAAATTATCAAAAG+TGG	+	chr3_3:58604344-58604363	None:intergenic	30.0%
GATATACCGTGATAATTTAG+CGG	+	chr3_3:58604379-58604398	None:intergenic	30.0%
ACCATACAGATATAAGATTG+GGG	-	chr3_3:58604656-58604675	Msa0460930:CDS	30.0%
ATTGGATGTTTCAGATGAAT+TGG	-	chr3_3:58604689-58604708	Msa0460930:CDS	30.0%
GGAATTAGATGAATCTCAAA+AGG	-	chr3_3:58604860-58604879	Msa0460930:intron	30.0%
ATTAGATGAATCTCAAAAGG+AGG	-	chr3_3:58604863-58604882	Msa0460930:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_3	gene	58604034	58606643	58604034	ID=Msa0460930;Name=Msa0460930
chr3_3	mRNA	58604034	58606643	58604034	ID=Msa0460930-mRNA-1;Parent=Msa0460930;Name=Msa0460930-mRNA-1;_AED=0.13;_eAED=0.13;_QI=80\|1\|1\|1\|1\|1\|5\|569\|195
chr3_3	exon	58604034	58604767	58604034	ID=Msa0460930-mRNA-1:exon:12230;Parent=Msa0460930-mRNA-1
chr3_3	exon	58605094	58605222	58605094	ID=Msa0460930-mRNA-1:exon:12229;Parent=Msa0460930-mRNA-1
chr3_3	exon	58605742	58605825	58605742	ID=Msa0460930-mRNA-1:exon:12228;Parent=Msa0460930-mRNA-1
chr3_3	exon	58605916	58606027	58605916	ID=Msa0460930-mRNA-1:exon:12227;Parent=Msa0460930-mRNA-1
chr3_3	exon	58606466	58606643	58606466	ID=Msa0460930-mRNA-1:exon:12226;Parent=Msa0460930-mRNA-1
chr3_3	five_prime_UTR	58606564	58606643	58606564	ID=Msa0460930-mRNA-1:five_prime_utr;Parent=Msa0460930-mRNA-1
chr3_3	CDS	58606466	58606563	58606466	ID=Msa0460930-mRNA-1:cds;Parent=Msa0460930-mRNA-1
chr3_3	CDS	58605916	58606027	58605916	ID=Msa0460930-mRNA-1:cds;Parent=Msa0460930-mRNA-1
chr3_3	CDS	58605742	58605825	58605742	ID=Msa0460930-mRNA-1:cds;Parent=Msa0460930-mRNA-1
chr3_3	CDS	58605094	58605222	58605094	ID=Msa0460930-mRNA-1:cds;Parent=Msa0460930-mRNA-1
chr3_3	CDS	58604603	58604767	58604603	ID=Msa0460930-mRNA-1:cds;Parent=Msa0460930-mRNA-1
chr3_3	three_prime_UTR	58604034	58604602	58604034	ID=Msa0460930-mRNA-1:three_prime_utr;Parent=Msa0460930-mRNA-1

Gene Sequence

>Msa0460930

ATGGTTGCTTTGGTTTCTCAAGAAAATGTTGTCACATTGGTTTCTCGTACTGGTAGAGAGTTACAACGTTATAGAAAAGGTCGTCGTCAAGTTGTTGGATGTATACCATACAGATATAAGATTGGGGAGAAAAATTCATTGGATGTTTCAGATGAATTGGAAGTTCTTGTTATAAGTTCTCAGAAAGGCAAAGGGATGCTATTTCCAAAGGGAGGTTGGGAATTAGATGAATCTCAAAAGGAGGCAGCTTTGAGAGAAACCATGGAGGAAGCTGGTGTACGAGGCATTGTTGAGGGTAAATTGGGTAAATGGAGTTTCAAGAGTAAAACATATGATACTTTATATGATGGTTACATGTTTCCTCTACTTGTTCAAGAGGAATTGGAGTTCTGGCCTGAACAAAACCTTCGTCAAAGAAGATGGATGAGTATATCTGAAGCAAGAGATGTTTGTCAACACTGGTGGATGAAAGAGGCTTTGGACAGACTAGTAAACCGTCTCTTAGGTCAAAAACTTGGTTCTGAGAAGCAAGTTCTACGTCCTCTAAATTGCATTGGAGATGCCAAATCTGACTTGTTAAAAGTGTAA

Protein sequence

>Msa0460930

MVALVSQENVVTLVSRTGRELQRYRKGRRQVVGCIPYRYKIGEKNSLDVSDELEVLVISSQKGKGMLFPKGGWELDESQKEAALRETMEEAGVRGIVEGKLGKWSFKSKTYDTLYDGYMFPLLVQEELEFWPEQNLRQRRWMSISEARDVCQHWWMKEALDRLVNRLLGQKLGSEKQVLRPLNCIGDAKSDLLKV*