AlfalfaGEDB | Msa1208340

M. sativa cultivar zhongmu-4 / Msa1208340

KEGG: -
GO: GO:0003674 GO:0005488 GO:0030246
PFAM: PF14299.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1208340	XP_013457676.1	95.105	286	14	0	1	286	1	286	0.0	552

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1208340	sp\|Q9LN77\|P2A12_ARATH	68.030	269	82	3	16	282	25	291	6.17e-134	383

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006700	Msa1208340	0.823882	1.090269e-53	-8.615850e-47
Msa0015100	Msa1208340	0.986452	7.784482e-167	-8.615850e-47
Msa0021490	Msa1208340	0.809386	1.941765e-50	-8.615850e-47
Msa0049770	Msa1208340	0.994121	9.826061e-205	-8.615850e-47
Msa0051200	Msa1208340	0.828298	9.717898e-55	-8.615850e-47
Msa0051460	Msa1208340	0.842320	2.778873e-58	-8.615850e-47
Msa0093440	Msa1208340	0.813474	2.513116e-51	-8.615850e-47
Msa0094940	Msa1208340	-0.826631	2.440067e-54	-8.615850e-47
Msa0101840	Msa1208340	0.837068	6.463643e-57	-8.615850e-47
Msa0111310	Msa1208340	0.815536	8.792373e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1208340	MtrunA17_Chr4g0057281	95.105	286	14	0	1	286	1	286	0.0	552
Msa1208340	MtrunA17_Chr5g0397981	73.868	287	70	3	1	286	19	301	1.39e-158	443
Msa1208340	MtrunA17_Chr8g0350401	60.854	281	103	2	1	274	1	281	2.94e-126	361
Msa1208340	MtrunA17_Chr1g0203751	62.308	260	97	1	19	278	28	286	7.01e-121	347
Msa1208340	MtrunA17_Chr6g0472801	58.672	271	110	2	13	282	16	285	2.76e-117	338
Msa1208340	MtrunA17_Chr7g0265551	55.670	291	117	2	1	280	73	362	7.85e-113	330
Msa1208340	MtrunA17_Chr7g0272101	26.642	274	168	7	22	278	4	261	9.35e-29	110
Msa1208340	MtrunA17_Chr4g0010881	27.132	258	165	5	22	277	4	240	4.80e-27	105
Msa1208340	MtrunA17_Chr1g0192371	25.862	290	165	9	18	273	7	280	3.65e-22	93.2
Msa1208340	MtrunA17_Chr7g0272091	25.357	280	182	5	18	273	6	282	2.37e-21	91.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1208340	AT1G12710.1	68.030	269	82	3	16	282	25	291	6.27e-135	383
Msa1208340	AT3G61060.1	62.782	266	97	2	14	278	20	284	6.48e-127	363
Msa1208340	AT1G12710.2	58.842	311	82	4	16	282	25	333	3.21e-126	363
Msa1208340	AT3G61060.2	62.547	267	97	3	14	278	20	285	3.12e-125	359
Msa1208340	AT1G63090.1	65.769	260	86	2	16	274	23	280	1.20e-122	352
Msa1208340	AT5G52120.1	52.174	276	127	1	7	277	9	284	4.94e-104	305
Msa1208340	AT3G53000.1	54.104	268	110	3	17	273	19	284	1.07e-99	294
Msa1208340	AT2G02360.1	36.129	155	85	4	10	161	8	151	5.80e-25	101
Msa1208340	AT2G02340.1	31.429	175	113	3	5	176	22	192	9.88e-23	95.9
Msa1208340	AT2G02230.1	34.266	143	87	2	22	163	35	171	4.64e-21	91.7

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Find 77 sgRNAs with CRISPR-Local

Find 312 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGGCGCTATTACCATGC+CGG	0.633820	8_1:+66381289	Msa1208340:CDS
AACCCTTCTTCCAAATCTCT+TGG	0.453456	8_1:-66381158	None:intergenic
AAGAAGCACCATGAAAAGCA+CGG	0.655921	8_1:-66379894	None:intergenic
AAGCTGTGTAGCTGTTATAA+TGG	0.396206	8_1:+66379828	Msa1208340:CDS
AATAAGCTATATGTTCCTGC+TGG	0.503889	8_1:-66381109	None:intergenic
AATTATAACATCATAATTAG+AGG	0.373488	8_1:-66379947	None:intergenic
AATTCTCCTAAACTTGGTTT+TGG	0.321554	8_1:-66379799	None:intergenic
ACAAATCTGGTGGTTTGAAG+TGG	0.565964	8_1:+66381063	Msa1208340:CDS
ACACAGAACATGTTCATGGT+TGG	0.524349	8_1:+66381185	Msa1208340:CDS
AGAATATTGTATACTTGTGC+CGG	0.575065	8_1:+66381700	Msa1208340:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCATAATTAGAGGGAAGTT+TGG	-	chr8_1:66379941-66379960	None:intergenic	30.0%
TGTGAACTATGAAGTTCAAA+GGG	+	chr8_1:66380310-66380329	Msa1208340:intron	30.0%
AGCTAGAAATGATAGCTTAT+AGG	+	chr8_1:66380614-66380633	Msa1208340:intron	30.0%
GACAGCAAATATGTTATGTT+TGG	-	chr8_1:66380682-66380701	None:intergenic	30.0%
AAAGCTAAGTATTTGACCTT+TGG	-	chr8_1:66380776-66380795	None:intergenic	30.0%
GAAGAGAAAGATATTGATGA+AGG	+	chr8_1:66380818-66380837	Msa1208340:intron	30.0%
TTAACCTTTCTAAACTCACT+AGG	-	chr8_1:66381427-66381446	None:intergenic	30.0%
TTTGAACTACTCTTAACCTA+AGG	+	chr8_1:66381453-66381472	Msa1208340:exon	30.0%
CTATGCATGTATCAAAGATT+AGG	+	chr8_1:66381495-66381514	Msa1208340:three_prime_UTR	30.0%
AGAATATTGTATACTTGTGC+CGG	+	chr8_1:66381700-66381719	Msa1208340:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	66379568	66381721	66379568	ID=Msa1208340;Name=Msa1208340
chr8_1	mRNA	66379568	66381721	66379568	ID=Msa1208340-mRNA-1;Parent=Msa1208340;Name=Msa1208340-mRNA-1;_AED=0.20;_eAED=0.20;_QI=189\|1\|1\|1\|1\|1\|3\|253\|286
chr8_1	exon	66379568	66380068	66379568	ID=Msa1208340-mRNA-1:exon:21798;Parent=Msa1208340-mRNA-1
chr8_1	exon	66380147	66380265	66380147	ID=Msa1208340-mRNA-1:exon:21799;Parent=Msa1208340-mRNA-1
chr8_1	exon	66381039	66381721	66381039	ID=Msa1208340-mRNA-1:exon:21800;Parent=Msa1208340-mRNA-1
chr8_1	five_prime_UTR	66379568	66379756	66379568	ID=Msa1208340-mRNA-1:five_prime_utr;Parent=Msa1208340-mRNA-1
chr8_1	CDS	66379757	66380068	66379757	ID=Msa1208340-mRNA-1:cds;Parent=Msa1208340-mRNA-1
chr8_1	CDS	66380147	66380265	66380147	ID=Msa1208340-mRNA-1:cds;Parent=Msa1208340-mRNA-1
chr8_1	CDS	66381039	66381468	66381039	ID=Msa1208340-mRNA-1:cds;Parent=Msa1208340-mRNA-1
chr8_1	three_prime_UTR	66381469	66381721	66381469	ID=Msa1208340-mRNA-1:three_prime_utr;Parent=Msa1208340-mRNA-1

Gene Sequence

>Msa1208340

ATGGGTATCTTGTTCTCAGTTTCATCCCAATCATCATCATCACCAAAACCAAGTTTAGGAGAATTACCAGAAAGCTGTGTAGCTGTTATAATGGAATACTTGGATCCTCCTCAGATTTGCAAATTAGCTTCTCTCAACCGTGCTTTTCATGGTGCTTCTTTGGCTGATTTTGTTTGGGAATCCAAACTTCCCTCTAATTATGATGTTATAATTCAAAAAATCTTTGATGGTTCTTCTTTTCCTTCTCATTTGGGGAAAAGAGGGATTTATTCAAGACTTTGTAGCTTCAATACTTTCGATGAAGGAACCAAGAAAGTTTGGTTGGATAGAAGCATGGGTAAAGTGTGTTTGTCTATATCGGCTAAAGGGTTGTCAATAACTGGGATTGATGATCGAAGATATTGGAACCATATTCCCACTGAAGAATCAAGATTCAATAATGTTGCGTATCTCCAACAAATCTGGTGGTTTGAAGTGGACGGTGAAATTGAGTTCCCATTTCCAGCAGGAACATATAGCTTATTCTTCCGAATACATTTAGGGAAACCCGCCAAGAGATTTGGAAGAAGGGTTTGCAACACAGAACATGTTCATGGTTGGGATGTAAAGCCTGTTAGATTCCAACTATGGACTTCAGATGATCAATATGATTCATCGCAATGTTTTTTGAAAGGTCCAGGTAAATGGCGCTATTACCATGCCGGCGATTTTGTGGTTGAAGATGGTAATGTATCAACAAAAGTAAAATTCTCTCTGACTCAAATTGATTGCACACACACTAAAGGTGGTCTCTGTTTAGATTCTGTGTTTGTGTATCCTAGTGAGTTTAGAAAGGTTAAAGAATTTTTGAACTACTCTTAA

Protein sequence

>Msa1208340

MGILFSVSSQSSSSPKPSLGELPESCVAVIMEYLDPPQICKLASLNRAFHGASLADFVWESKLPSNYDVIIQKIFDGSSFPSHLGKRGIYSRLCSFNTFDEGTKKVWLDRSMGKVCLSISAKGLSITGIDDRRYWNHIPTEESRFNNVAYLQQIWWFEVDGEIEFPFPAGTYSLFFRIHLGKPAKRFGRRVCNTEHVHGWDVKPVRFQLWTSDDQYDSSQCFLKGPGKWRYYHAGDFVVEDGNVSTKVKFSLTQIDCTHTKGGLCLDSVFVYPSEFRKVKEFLNYS*