AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45913


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45913.t1 MTR_7g057160 96.491 171 6 0 1 171 1 171 6.09e-122 341
MS.gene45913.t1 MTR_3g436010 46.154 156 78 1 16 171 169 318 1.69e-33 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45913.t1 AT3G17609 60.000 130 47 2 39 167 22 147 7.06e-44 142
MS.gene45913.t1 AT3G17609 61.157 121 42 2 48 167 2 118 3.58e-41 135
MS.gene45913.t1 AT3G17609 74.444 90 22 1 79 167 43 132 4.84e-40 132
MS.gene45913.t1 AT3G17609 74.444 90 22 1 79 167 44 133 5.04e-40 132
MS.gene45913.t1 AT5G11260 48.175 137 54 3 31 161 26 151 5.65e-33 115
MS.gene45913.t1 AT5G11260 48.120 133 52 3 31 157 26 147 2.95e-30 108

Find 39 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGATACGTTCTTCTAAAT+TGG 0.194375 7.2:+45766443 None:intergenic
CTTCATCTTCATCTTCTTCA+TGG 0.213050 7.2:-45768140 MS.gene45913:CDS
TCTTCCACGGCGGCGCTTGC+CGG 0.245919 7.2:+45766969 None:intergenic
CTTCCACGGCGGCGCTTGCC+GGG 0.275869 7.2:+45766970 None:intergenic
CAAATAGGTTGCTAAGGAAT+AGG 0.306771 7.2:-45766551 MS.gene45913:intron
TCATTGCTCTCATCAACTTT+GGG 0.320674 7.2:+45766250 None:intergenic
TTCATTGCTCTCATCAACTT+TGG 0.343653 7.2:+45766249 None:intergenic
CATTGTGTACCTTCCACATC+TGG 0.376399 7.2:+45767078 None:intergenic
TTCCTTCATCTCAGTATCAT+TGG 0.395557 7.2:+45768182 None:intergenic
TCGGAGCATGGCGTTCTCAT+TGG 0.400151 7.2:+45766413 None:intergenic
ATGAAGGAAGCTGCAACAAC+TGG 0.409191 7.2:-45768168 MS.gene45913:CDS
TTTGATTTGTTAACTCAGAA+TGG 0.409238 7.2:+45767046 None:intergenic
CGTCTATGTTCCTTGTCTGC+AGG 0.409298 7.2:+45766943 None:intergenic
CCTATGCCTCCACATAGATT+TGG 0.415132 7.2:-45768105 MS.gene45913:intron
AAATAGGTTGCTAAGGAATA+GGG 0.428080 7.2:-45766550 MS.gene45913:intron
CTTCATCTTCTTCATGGAGT+AGG 0.466765 7.2:-45768134 MS.gene45913:CDS
CAACTTCAAGCAGGCTTCCC+CGG 0.470731 7.2:-45766988 MS.gene45913:CDS
GTCTATGTTCCTTGTCTGCA+GGG 0.478530 7.2:+45766944 None:intergenic
CAGTTACATTGCTTTGATTG+TGG 0.494434 7.2:+45767016 None:intergenic
AGCCAATGATACTGAGATGA+AGG 0.496590 7.2:-45768184 MS.gene45913:CDS
TGTCTGCAGGGTTTCTTCCA+CGG 0.500164 7.2:+45766956 None:intergenic
AAAGTTGATGAGAGCAATGA+AGG 0.515891 7.2:-45766247 MS.gene45913:CDS
TTATGTTACCTTTCGGAGCA+TGG 0.519796 7.2:+45766401 None:intergenic
TTCCACGGCGGCGCTTGCCG+GGG 0.522944 7.2:+45766971 None:intergenic
CCAAATCTATGTGGAGGCAT+AGG 0.526031 7.2:+45768105 None:intergenic
AGGAACATAGACGTCTCAAA+AGG 0.535376 7.2:-45766933 MS.gene45913:intron
GTGTTACAAATAGGTTGCTA+AGG 0.552163 7.2:-45766557 MS.gene45913:intron
TGGAAGAAACCCTGCAGACA+AGG 0.552255 7.2:-45766953 MS.gene45913:CDS
CATGAAGAAGATGAAGATGA+AGG 0.570746 7.2:+45768141 None:intergenic
GTTGCTAAGGAATAGGGTCT+CGG 0.571634 7.2:-45766544 MS.gene45913:CDS
AATCTATGTGGAGGCATAGG+AGG 0.581180 7.2:+45768108 None:intergenic
ACTGATGCTCAACTTCAAGC+AGG 0.591244 7.2:-45766997 MS.gene45913:CDS
TTCCCCGGCAAGCGCCGCCG+TGG 0.608180 7.2:-45766973 MS.gene45913:CDS
TGAGAACGCCATGCTCCGAA+AGG 0.615500 7.2:-45766409 MS.gene45913:intron
CACTTACCAAATCTATGTGG+AGG 0.634191 7.2:+45768099 None:intergenic
TCTGTTCGAAGTTCCAGATG+TGG 0.639704 7.2:-45767091 MS.gene45913:CDS
TGACACTTACCAAATCTATG+TGG 0.640404 7.2:+45768096 None:intergenic
CTGCAGGGTTTCTTCCACGG+CGG 0.647406 7.2:+45766959 None:intergenic
TTCGAAGTTCCAGATGTGGA+AGG 0.651950 7.2:-45767087 MS.gene45913:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATAATTGTAATATGTAT+TGG + chr7.2:45767707-45767726 None:intergenic 10.0%
!! TTGTTAAATAAAAATTTAAC+GGG + chr7.2:45766751-45766770 None:intergenic 10.0%
!! AAGAATATTAAGTATATTGT+TGG - chr7.2:45766601-45766620 MS.gene45913:intron 15.0%
!! AATGTCAAATTAAAATTTGA+AGG + chr7.2:45767213-45767232 None:intergenic 15.0%
!! ATGTCAAATTAAAATTTGAA+GGG + chr7.2:45767212-45767231 None:intergenic 15.0%
!! CAAAATTGTTACTATATATT+AGG - chr7.2:45767151-45767170 MS.gene45913:intron 15.0%
!! GGTATATATATAATATAAGT+TGG - chr7.2:45766665-45766684 MS.gene45913:intron 15.0%
!! TCAAAATTTGCATTGAATTT+CGG - chr7.2:45766966-45766985 MS.gene45913:CDS 20.0%
!! TCAAATTGTTGATGTAAAAA+CGG + chr7.2:45766826-45766845 None:intergenic 20.0%
!!! ACAAGTTGTTTTTAAATGAT+CGG + chr7.2:45766630-45766649 None:intergenic 20.0%
!!! TAATATGGACTAAATTGAAT+TGG + chr7.2:45767241-45767260 None:intergenic 20.0%
! AAAACAAGTCACGTTTAATA+TGG + chr7.2:45767256-45767275 None:intergenic 25.0%
! AACTTTGTGATATGATATTG+TGG + chr7.2:45766863-45766882 None:intergenic 25.0%
! ACTTGTTTCATGAAAAGTAA+AGG - chr7.2:45766644-45766663 MS.gene45913:intron 25.0%
! ATAACATCATGTTGATCAAA+TGG - chr7.2:45768121-45768140 MS.gene45913:CDS 25.0%
! ATATGCGTGTGTATATTATA+AGG + chr7.2:45766706-45766725 None:intergenic 25.0%
! GTTAAGATTATGTTACCTTT+CGG + chr7.2:45768050-45768069 None:intergenic 25.0%
! TACACACGCATATATTAAAT+TGG - chr7.2:45766713-45766732 MS.gene45913:intron 25.0%
! TATGAAGAATCTTGTAAGTT+TGG + chr7.2:45766530-45766549 None:intergenic 25.0%
! TATGCGTGTGTATATTATAA+GGG + chr7.2:45766705-45766724 None:intergenic 25.0%
! TCAAATGGAAATTTATTCGA+TGG - chr7.2:45768136-45768155 MS.gene45913:CDS 25.0%
!! AAATAAAAGACCTTAACTTG+AGG + chr7.2:45766430-45766449 None:intergenic 25.0%
!! AAATGACTTGTGTTACAAAT+AGG - chr7.2:45767875-45767894 MS.gene45913:intron 25.0%
!! CATATTTTATCCTCAAGTTA+AGG - chr7.2:45766417-45766436 MS.gene45913:CDS 25.0%
!!! ATATAAGTTGGTCCATATTA+AGG - chr7.2:45766677-45766696 MS.gene45913:intron 25.0%
!!! TATAAGTTGGTCCATATTAA+GGG - chr7.2:45766678-45766697 MS.gene45913:intron 25.0%
!!! TTTGATTTGTTAACTCAGAA+TGG + chr7.2:45767398-45767417 None:intergenic 25.0%
AAATAGGTTGCTAAGGAATA+GGG - chr7.2:45767891-45767910 MS.gene45913:intron 30.0%
AACTGCAGACAATTCAAATT+TGG - chr7.2:45767042-45767061 MS.gene45913:CDS 30.0%
AATAGTGACTATAGAGTGTA+AGG + chr7.2:45767568-45767587 None:intergenic 30.0%
AATCTTGTAAGTTTGGACTT+TGG + chr7.2:45766523-45766542 None:intergenic 30.0%
ACATTAACATGTGCTAACAT+TGG + chr7.2:45767068-45767087 None:intergenic 30.0%
ATGATTATCGATCATTGTGA+AGG + chr7.2:45766892-45766911 None:intergenic 30.0%
GTCTGACTTTACTTATCTTT+CGG - chr7.2:45767820-45767839 MS.gene45913:intron 30.0%
TGAGATACGTTCTTCTAAAT+TGG + chr7.2:45768001-45768020 None:intergenic 30.0%
!! ATAAGTTGGTCCATATTAAG+GGG - chr7.2:45766679-45766698 MS.gene45913:intron 30.0%
!!! CGTACACTTTTTTTCTTTCA+CGG + chr7.2:45767933-45767952 None:intergenic 30.0%
!!! GTACACTTTTTTTCTTTCAC+GGG + chr7.2:45767932-45767951 None:intergenic 30.0%
ATAGACGTCTCAAAAGGTAA+GGG - chr7.2:45767514-45767533 MS.gene45913:intron 35.0%
ATTATAAGGGCCCCTTAATA+TGG + chr7.2:45766692-45766711 None:intergenic 35.0%
ATTATCGATCATTGTGAAGG+TGG + chr7.2:45766889-45766908 None:intergenic 35.0%
CAAATAGGTTGCTAAGGAAT+AGG - chr7.2:45767890-45767909 MS.gene45913:intron 35.0%
CAGTTACATTGCTTTGATTG+TGG + chr7.2:45767428-45767447 None:intergenic 35.0%
CATGAAGAAGATGAAGATGA+AGG + chr7.2:45766303-45766322 None:intergenic 35.0%
CTCAAACGATTGTTGAACTT+CGG - chr7.2:45767755-45767774 MS.gene45913:intron 35.0%
CTTCATCTTCATCTTCTTCA+TGG - chr7.2:45766301-45766320 MS.gene45913:intron 35.0%
GTGTTACAAATAGGTTGCTA+AGG - chr7.2:45767884-45767903 MS.gene45913:intron 35.0%
TATTGATAGGCTCTTGACTT+AGG + chr7.2:45766473-45766492 None:intergenic 35.0%
TCATTGCTCTCATCAACTTT+GGG + chr7.2:45768194-45768213 None:intergenic 35.0%
TGACACTTACCAAATCTATG+TGG + chr7.2:45766348-45766367 None:intergenic 35.0%
TTCATTGCTCTCATCAACTT+TGG + chr7.2:45768195-45768214 None:intergenic 35.0%
TTCCTTCATCTCAGTATCAT+TGG + chr7.2:45766262-45766281 None:intergenic 35.0%
!! AAAGTTGATGAGAGCAATGA+AGG - chr7.2:45768194-45768213 MS.gene45913:CDS 35.0%
!! TTCGATGGTTTTACTTGTAC+AGG - chr7.2:45768151-45768170 MS.gene45913:CDS 35.0%
AGCCAATGATACTGAGATGA+AGG - chr7.2:45766257-45766276 MS.gene45913:CDS 40.0%
AGGAACATAGACGTCTCAAA+AGG - chr7.2:45767508-45767527 MS.gene45913:intron 40.0%
CACTTACCAAATCTATGTGG+AGG + chr7.2:45766345-45766364 None:intergenic 40.0%
CATAGACGTCTCAAAAGGTA+AGG - chr7.2:45767513-45767532 MS.gene45913:intron 40.0%
CTTCATCTTCTTCATGGAGT+AGG - chr7.2:45766307-45766326 MS.gene45913:intron 40.0%
TTATGTTACCTTTCGGAGCA+TGG + chr7.2:45768043-45768062 None:intergenic 40.0%
! TTTTTCTTTCACGGGCTTGT+TGG + chr7.2:45767924-45767943 None:intergenic 40.0%
!! TTTTCTTTCACGGGCTTGTT+GGG + chr7.2:45767923-45767942 None:intergenic 40.0%
ACTGATGCTCAACTTCAAGC+AGG - chr7.2:45767444-45767463 MS.gene45913:intron 45.0%
AGATCTTCATCGCTATCGTC+TGG + chr7.2:45767333-45767352 None:intergenic 45.0%
ATGAAGGAAGCTGCAACAAC+TGG - chr7.2:45766273-45766292 MS.gene45913:intron 45.0%
CATTGTGTACCTTCCACATC+TGG + chr7.2:45767366-45767385 None:intergenic 45.0%
CCTATGCCTCCACATAGATT+TGG - chr7.2:45766336-45766355 MS.gene45913:intron 45.0%
GGTGACAGTTGGATCTAGAT+TGG + chr7.2:45766996-45767015 None:intergenic 45.0%
GTCTATGTTCCTTGTCTGCA+GGG + chr7.2:45767500-45767519 None:intergenic 45.0%
GTTGCTAAGGAATAGGGTCT+CGG - chr7.2:45767897-45767916 MS.gene45913:intron 45.0%
TAGATCCAACTGTCACCTGA+GGG - chr7.2:45766999-45767018 MS.gene45913:CDS 45.0%
TCTGTTCGAAGTTCCAGATG+TGG - chr7.2:45767350-45767369 MS.gene45913:intron 45.0%
TTCGAAGTTCCAGATGTGGA+AGG - chr7.2:45767354-45767373 MS.gene45913:intron 45.0%
! AATCTATGTGGAGGCATAGG+AGG + chr7.2:45766336-45766355 None:intergenic 45.0%
! CCAAATCTATGTGGAGGCAT+AGG + chr7.2:45766339-45766358 None:intergenic 45.0%
!! ATTGTTAAATAAAAATTTAA+CGG + chr7.2:45766752-45766771 None:intergenic 5.0%
!!! TTTTTTAATTAAATATTGAT+AGG + chr7.2:45766486-45766505 None:intergenic 5.0%
CGTCTATGTTCCTTGTCTGC+AGG + chr7.2:45767501-45767520 None:intergenic 50.0%
CTAGATCCAACTGTCACCTG+AGG - chr7.2:45766998-45767017 MS.gene45913:CDS 50.0%
TGGAAGAAACCCTGCAGACA+AGG - chr7.2:45767488-45767507 MS.gene45913:intron 50.0%
TGTCTGCAGGGTTTCTTCCA+CGG + chr7.2:45767488-45767507 None:intergenic 50.0%
CAACTTCAAGCAGGCTTCCC+CGG - chr7.2:45767453-45767472 MS.gene45913:intron 55.0%
TCACTCCCTCAGGTGACAGT+TGG + chr7.2:45767007-45767026 None:intergenic 55.0%
TCGGAGCATGGCGTTCTCAT+TGG + chr7.2:45768031-45768050 None:intergenic 55.0%
TCTCGTGCATTCACTCCCTC+AGG + chr7.2:45767017-45767036 None:intergenic 55.0%
TGAGAACGCCATGCTCCGAA+AGG - chr7.2:45768032-45768051 MS.gene45913:intron 55.0%
CTGCAGGGTTTCTTCCACGG+CGG + chr7.2:45767485-45767504 None:intergenic 60.0%
TCTTCCACGGCGGCGCTTGC+CGG + chr7.2:45767475-45767494 None:intergenic 70.0%
CTTCCACGGCGGCGCTTGCC+GGG + chr7.2:45767474-45767493 None:intergenic 75.0%
TTCCACGGCGGCGCTTGCCG+GGG + chr7.2:45767473-45767492 None:intergenic 75.0%
!! TTCCCCGGCAAGCGCCGCCG+TGG - chr7.2:45767468-45767487 MS.gene45913:intron 80.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 45766243 45768220 45766243 ID=MS.gene45913
chr7.2 mRNA 45766243 45768220 45766243 ID=MS.gene45913.t1;Parent=MS.gene45913
chr7.2 exon 45768106 45768220 45768106 ID=MS.gene45913.t1.exon1;Parent=MS.gene45913.t1
chr7.2 CDS 45768106 45768220 45768106 ID=cds.MS.gene45913.t1;Parent=MS.gene45913.t1
chr7.2 exon 45766934 45767129 45766934 ID=MS.gene45913.t1.exon2;Parent=MS.gene45913.t1
chr7.2 CDS 45766934 45767129 45766934 ID=cds.MS.gene45913.t1;Parent=MS.gene45913.t1
chr7.2 exon 45766410 45766566 45766410 ID=MS.gene45913.t1.exon3;Parent=MS.gene45913.t1
chr7.2 CDS 45766410 45766566 45766410 ID=cds.MS.gene45913.t1;Parent=MS.gene45913.t1
chr7.2 exon 45766243 45766290 45766243 ID=MS.gene45913.t1.exon4;Parent=MS.gene45913.t1
chr7.2 CDS 45766243 45766290 45766243 ID=cds.MS.gene45913.t1;Parent=MS.gene45913.t1
Gene Sequence

>MS.gene45913

ATGTCTCTTTCAAGAGCCAATGATACTGAGATGAAGGAAGCTGCAACAACTGGAGCACCTTCATCTTCATCTTCTTCATGGAGTAGGTTACCTCCTATGCCTCCACATAGATTTGGTAAGTGTCATTTTCAATTTTTTTTTAATTAGTTTATTTTATGCTCAAGTTTTTTGACTCATATTTTATCCTCAAGTTAAGGTCTTTTATTTTATTTTATTTTTGACATAATCCTAAGTCAAGAGCCTATCAATATTTAATTAAAAAAAATGATTAACTAATCCAAAGTCCAAACTTACAAGATTCTTCATAAAGTTTGTCAAAACAAAAAAAAAATATTAAGTATATTGTTTTTTTTTTTTTAAGAATATTAAGTATATTGTTGGATTCCGATCATTTAAAAACAACTTGTTTCATGAAAAGTAAAGGTATATATATAATATAAGTTGGTCCATATTAAGGGGCCCTTATAATATACACACGCATATATTAAATTGGTCTAACATTCTACCCGTTAAATTTTTATTTAACAATTATACTTACATAGCATCATTCTTTTTAGATATGATTGAATGCATGTATAAACCGTTTTTACATCAACAATTTGACAAATTTAGATTATCCACAATATCATATCACAAAGTTTATCCACCTTCACAATGATCGATAATCATTAGATTGAGATCGAAAGATCTAGATTTCAAACTTATTTTAACTTTAAAAAATAATCAAAATTTGCATTGAATTTCGGAGCTCCAATCTAGATCCAACTGTCACCTGAGGGAGTGAATGCACGAGATTGCGAACTGCAGACAATTCAAATTTGGCCAATGTTAGCACATGTTAATGTACTCGAAATATAAAAATTCGTATGTTCAACTTTTTAAAATTCAAAATATTGTTTTTATCAATGCAAAATTGTTACTATATATTAGGTTCTTTAACATTAGTACACAAGTTAGTTATAATGACCCTTCAAATTTTAATTTGACATTGTATGCCAATTCAATTTAGTCCATATTAAACGTGACTTGTTTTGTTTTGACAATACTAAATATGTCATGTTAATAAGTCATGATTTTTTTACAAATTCCAGACGATAGCGATGAAGATCTGTTCGAAGTTCCAGATGTGGAAGGTACACAATGTCAAAGTGTCCATTCTGAGTTAACAAATCAAAATAGTAACCACAATCAAAGCAATGTAACTGATGCTCAACTTCAAGCAGGCTTCCCCGGCAAGCGCCGCCGTGGAAGAAACCCTGCAGACAAGGAACATAGACGTCTCAAAAGGTAAGGGACACAAGTTAACACAAATTTTAGCTATACCTTACACTCTATAGTCACTATTATAAACAAACAACTATTTTTTAAATTCATTGAATAACTGATGTATTTTGTATATAATATAGACTATTGTATTAATTTCTACATTAAATTATCGAACTTTGTCACTTTAAGAAGATTCCAATACATATTACAATTATAATGTTATTCAAGAGTAATTGAGTTATTGAACTCAAACGATTGTTGAACTTCGGTCAAAGACGAATCATTCAGAATACTTAATGAGAACAATTTTAGTCTGACTTTACTTATCTTTCGGCAGAACTTCAGATTAAACTATCTAAAACTAATAAATGACTTGTGTTACAAATAGGTTGCTAAGGAATAGGGTCTCGGCCCAACAAGCCCGTGAAAGAAAAAAAGTGTACGTGAATGACTTAGAATCAAGAGCTAAAGAGTTTGAAGATAAGAACTCCAATTTAGAAGAACGTATCTCAACTTTGACCAATGAGAACGCCATGCTCCGAAAGGTAACATAATCTTAACTAAATATGTTTTAACAAATTGTTTGTAACTTACTATTTACTATAGAATTATATAACATCATGTTGATCAAATGGAAATTTATTCGATGGTTTTACTTGTACAGGTTCTTATGAACACTAGACCCAAAGTTGATGAGAGCAATGAAGGTTAG

Protein sequence

>MS.gene45913.t1

MSLSRANDTEMKEAATTGAPSSSSSSWSRLPPMPPHRFDDSDEDLFEVPDVEGTQCQSVHSELTNQNSNHNQSNVTDAQLQAGFPGKRRRGRNPADKEHRRLKRLLRNRVSAQQARERKKVYVNDLESRAKEFEDKNSNLEERISTLTNENAMLRKVLMNTRPKVDESNEG