Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93506.t1 | XP_003599006.2 | 90.8 | 163 | 4 | 2 | 1 | 163 | 38 | 189 | 1.40E-32 | 149.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93506.t1 | G7IZC1 | 90.8 | 163 | 4 | 2 | 1 | 163 | 38 | 189 | 1.0e-32 | 149.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene93506 | 0.862339 | 5.43E-64 | -1.69E-46 |
| MS.gene049171 | MS.gene93506 | 0.847647 | 1.01E-59 | -1.69E-46 |
| MS.gene049173 | MS.gene93506 | 0.812327 | 4.48E-51 | -1.69E-46 |
| MS.gene049217 | MS.gene93506 | 0.873582 | 1.32E-67 | -1.69E-46 |
| MS.gene049222 | MS.gene93506 | 0.874809 | 5.05E-68 | -1.69E-46 |
| MS.gene049232 | MS.gene93506 | 0.833106 | 6.45E-56 | -1.69E-46 |
| MS.gene049285 | MS.gene93506 | 0.863567 | 2.27E-64 | -1.69E-46 |
| MS.gene049320 | MS.gene93506 | 0.863339 | 2.67E-64 | -1.69E-46 |
| MS.gene04938 | MS.gene93506 | 0.817553 | 3.11E-52 | -1.69E-46 |
| MS.gene04939 | MS.gene93506 | 0.852848 | 3.53E-61 | -1.69E-46 |
| MS.gene049427 | MS.gene93506 | 0.82807 | 1.10E-54 | -1.69E-46 |
| MS.gene049488 | MS.gene93506 | 0.833018 | 6.79E-56 | -1.69E-46 |
| MS.gene049609 | MS.gene93506 | 0.865629 | 5.14E-65 | -1.69E-46 |
| MS.gene049633 | MS.gene93506 | 0.834222 | 3.40E-56 | -1.69E-46 |
| MS.gene049805 | MS.gene93506 | 0.889603 | 2.09E-73 | -1.69E-46 |
| MS.gene05010 | MS.gene93506 | 0.812366 | 4.40E-51 | -1.69E-46 |
| MS.gene050163 | MS.gene93506 | 0.86858 | 5.88E-66 | -1.69E-46 |
| MS.gene050165 | MS.gene93506 | 0.852698 | 3.89E-61 | -1.69E-46 |
| MS.gene050170 | MS.gene93506 | 0.813258 | 2.80E-51 | -1.69E-46 |
| MS.gene050323 | MS.gene93506 | 0.830121 | 3.51E-55 | -1.69E-46 |
| MS.gene050333 | MS.gene93506 | 0.808116 | 3.63E-50 | -1.69E-46 |
| MS.gene050381 | MS.gene93506 | -0.800972 | 1.12E-48 | -1.69E-46 |
| MS.gene050444 | MS.gene93506 | 0.806561 | 7.75E-50 | -1.69E-46 |
| MS.gene050496 | MS.gene93506 | 0.802249 | 6.14E-49 | -1.69E-46 |
| MS.gene050499 | MS.gene93506 | 0.807709 | 4.43E-50 | -1.69E-46 |
| MS.gene050504 | MS.gene93506 | 0.869632 | 2.68E-66 | -1.69E-46 |
| MS.gene050518 | MS.gene93506 | 0.845716 | 3.41E-59 | -1.69E-46 |
| MS.gene050538 | MS.gene93506 | 0.832858 | 7.44E-56 | -1.69E-46 |
| MS.gene050577 | MS.gene93506 | 0.867857 | 1.00E-65 | -1.69E-46 |
| MS.gene050593 | MS.gene93506 | 0.823362 | 1.44E-53 | -1.69E-46 |
| MS.gene050612 | MS.gene93506 | 0.863327 | 2.70E-64 | -1.69E-46 |
| MS.gene050647 | MS.gene93506 | -0.810062 | 1.39E-50 | -1.69E-46 |
| MS.gene050662 | MS.gene93506 | 0.80354 | 3.32E-49 | -1.69E-46 |
| MS.gene050675 | MS.gene93506 | 0.853781 | 1.90E-61 | -1.69E-46 |
| MS.gene050690 | MS.gene93506 | 0.810543 | 1.09E-50 | -1.69E-46 |
| MS.gene050699 | MS.gene93506 | 0.868298 | 7.24E-66 | -1.69E-46 |
| MS.gene050747 | MS.gene93506 | 0.865212 | 6.96E-65 | -1.69E-46 |
| MS.gene050772 | MS.gene93506 | 0.86068 | 1.74E-63 | -1.69E-46 |
| MS.gene05080 | MS.gene93506 | 0.830758 | 2.45E-55 | -1.69E-46 |
| MS.gene050868 | MS.gene93506 | 0.83701 | 6.69E-57 | -1.69E-46 |
| MS.gene050871 | MS.gene93506 | 0.839425 | 1.60E-57 | -1.69E-46 |
| MS.gene050950 | MS.gene93506 | 0.86422 | 1.42E-64 | -1.69E-46 |
| MS.gene050986 | MS.gene93506 | 0.860447 | 2.05E-63 | -1.69E-46 |
| MS.gene05100 | MS.gene93506 | 0.840773 | 7.11E-58 | -1.69E-46 |
| MS.gene05105 | MS.gene93506 | 0.809803 | 1.58E-50 | -1.69E-46 |
| MS.gene051167 | MS.gene93506 | -0.801814 | 7.54E-49 | -1.69E-46 |
| MS.gene051317 | MS.gene93506 | 0.817348 | 3.46E-52 | -1.69E-46 |
| MS.gene051387 | MS.gene93506 | 0.808778 | 2.62E-50 | -1.69E-46 |
| MS.gene051509 | MS.gene93506 | 0.813105 | 3.03E-51 | -1.69E-46 |
| MS.gene051551 | MS.gene93506 | 0.848673 | 5.28E-60 | -1.69E-46 |
| MS.gene051666 | MS.gene93506 | 0.852082 | 5.83E-61 | -1.69E-46 |
| MS.gene051692 | MS.gene93506 | 0.835606 | 1.52E-56 | -1.69E-46 |
| MS.gene051694 | MS.gene93506 | 0.818801 | 1.62E-52 | -1.69E-46 |
| MS.gene051734 | MS.gene93506 | 0.806506 | 7.96E-50 | -1.69E-46 |
| MS.gene051810 | MS.gene93506 | 0.865334 | 6.37E-65 | -1.69E-46 |
| MS.gene051838 | MS.gene93506 | 0.833357 | 5.59E-56 | -1.69E-46 |
| MS.gene051858 | MS.gene93506 | 0.821185 | 4.62E-53 | -1.69E-46 |
| MS.gene051941 | MS.gene93506 | 0.807106 | 5.94E-50 | -1.69E-46 |
| MS.gene052164 | MS.gene93506 | 0.892088 | 2.19E-74 | -1.69E-46 |
| MS.gene052271 | MS.gene93506 | 0.819721 | 1.00E-52 | -1.69E-46 |
| MS.gene052379 | MS.gene93506 | 0.816651 | 4.95E-52 | -1.69E-46 |
| MS.gene05240 | MS.gene93506 | -0.805009 | 1.64E-49 | -1.69E-46 |
| MS.gene052414 | MS.gene93506 | 0.8132 | 2.89E-51 | -1.69E-46 |
| MS.gene052421 | MS.gene93506 | 0.830612 | 2.66E-55 | -1.69E-46 |
| MS.gene052481 | MS.gene93506 | 0.814312 | 1.64E-51 | -1.69E-46 |
| MS.gene052482 | MS.gene93506 | 0.817866 | 2.64E-52 | -1.69E-46 |
| MS.gene052484 | MS.gene93506 | 0.821343 | 4.25E-53 | -1.69E-46 |
| MS.gene052486 | MS.gene93506 | 0.8259 | 3.64E-54 | -1.69E-46 |
| MS.gene052501 | MS.gene93506 | 0.838581 | 2.64E-57 | -1.69E-46 |
| MS.gene052503 | MS.gene93506 | 0.866198 | 3.40E-65 | -1.69E-46 |
| MS.gene052505 | MS.gene93506 | 0.837162 | 6.12E-57 | -1.69E-46 |
| MS.gene052615 | MS.gene93506 | 0.915675 | 4.44E-85 | -1.69E-46 |
| MS.gene052823 | MS.gene93506 | 0.865109 | 7.49E-65 | -1.69E-46 |
| MS.gene052825 | MS.gene93506 | 0.859759 | 3.31E-63 | -1.69E-46 |
| MS.gene052916 | MS.gene93506 | 0.806036 | 1.00E-49 | -1.69E-46 |
| MS.gene052966 | MS.gene93506 | 0.828674 | 7.88E-55 | -1.69E-46 |
| MS.gene052982 | MS.gene93506 | 0.823426 | 1.39E-53 | -1.69E-46 |
| MS.gene053203 | MS.gene93506 | 0.853991 | 1.66E-61 | -1.69E-46 |
| MS.gene053207 | MS.gene93506 | 0.852756 | 3.75E-61 | -1.69E-46 |
| MS.gene053213 | MS.gene93506 | 0.884404 | 1.97E-71 | -1.69E-46 |
| MS.gene053286 | MS.gene93506 | 0.881265 | 2.77E-70 | -1.69E-46 |
| MS.gene053322 | MS.gene93506 | 0.81488 | 1.23E-51 | -1.69E-46 |
| MS.gene053436 | MS.gene93506 | 0.854126 | 1.51E-61 | -1.69E-46 |
| MS.gene053480 | MS.gene93506 | 0.845033 | 5.23E-59 | -1.69E-46 |
| MS.gene053574 | MS.gene93506 | 0.868472 | 6.37E-66 | -1.69E-46 |
| MS.gene053579 | MS.gene93506 | 0.81203 | 5.20E-51 | -1.69E-46 |
| MS.gene053626 | MS.gene93506 | 0.843696 | 1.20E-58 | -1.69E-46 |
| MS.gene053632 | MS.gene93506 | 0.896956 | 2.24E-76 | -1.69E-46 |
| MS.gene053824 | MS.gene93506 | 0.831415 | 1.69E-55 | -1.69E-46 |
| MS.gene053912 | MS.gene93506 | 0.819129 | 1.37E-52 | -1.69E-46 |
| MS.gene053915 | MS.gene93506 | 0.803237 | 3.84E-49 | -1.69E-46 |
| MS.gene053916 | MS.gene93506 | 0.819108 | 1.38E-52 | -1.69E-46 |
| MS.gene054003 | MS.gene93506 | 0.818421 | 1.98E-52 | -1.69E-46 |
| MS.gene054408 | MS.gene93506 | 0.850744 | 1.39E-60 | -1.69E-46 |
| MS.gene054595 | MS.gene93506 | 0.848644 | 5.37E-60 | -1.69E-46 |
| MS.gene054600 | MS.gene93506 | 0.856127 | 3.97E-62 | -1.69E-46 |
| MS.gene054642 | MS.gene93506 | 0.87098 | 9.68E-67 | -1.69E-46 |
| MS.gene054706 | MS.gene93506 | -0.826356 | 2.84E-54 | -1.69E-46 |
| MS.gene05470 | MS.gene93506 | 0.861158 | 1.25E-63 | -1.69E-46 |
| MS.gene054742 | MS.gene93506 | -0.809711 | 1.65E-50 | -1.69E-46 |
| MS.gene054927 | MS.gene93506 | 0.843738 | 1.16E-58 | -1.69E-46 |
| MS.gene054954 | MS.gene93506 | 0.8635 | 2.38E-64 | -1.69E-46 |
| MS.gene05505 | MS.gene93506 | 0.839951 | 1.17E-57 | -1.69E-46 |
| MS.gene055206 | MS.gene93506 | 0.832306 | 1.02E-55 | -1.69E-46 |
| MS.gene055289 | MS.gene93506 | 0.818679 | 1.73E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene93506 | 0.857195 | 1.93E-62 | -1.69E-46 |
| MS.gene055397 | MS.gene93506 | 0.808783 | 2.61E-50 | -1.69E-46 |
| MS.gene055486 | MS.gene93506 | 0.822989 | 1.76E-53 | -1.69E-46 |
| MS.gene055499 | MS.gene93506 | 0.81646 | 5.47E-52 | -1.69E-46 |
| MS.gene055502 | MS.gene93506 | 0.883342 | 4.87E-71 | -1.69E-46 |
| MS.gene055577 | MS.gene93506 | 0.868451 | 6.47E-66 | -1.69E-46 |
| MS.gene055629 | MS.gene93506 | 0.834684 | 2.60E-56 | -1.69E-46 |
| MS.gene055647 | MS.gene93506 | 0.818735 | 1.68E-52 | -1.69E-46 |
| MS.gene055722 | MS.gene93506 | 0.834324 | 3.20E-56 | -1.69E-46 |
| MS.gene055748 | MS.gene93506 | 0.806606 | 7.58E-50 | -1.69E-46 |
| MS.gene055750 | MS.gene93506 | 0.807265 | 5.50E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene93506 | 0.847323 | 1.24E-59 | -1.69E-46 |
| MS.gene055909 | MS.gene93506 | 0.809552 | 1.79E-50 | -1.69E-46 |
| MS.gene055911 | MS.gene93506 | 0.812093 | 5.04E-51 | -1.69E-46 |
| MS.gene055941 | MS.gene93506 | 0.861524 | 9.66E-64 | -1.69E-46 |
| MS.gene056040 | MS.gene93506 | 0.818852 | 1.58E-52 | -1.69E-46 |
| MS.gene056046 | MS.gene93506 | 0.802116 | 6.53E-49 | -1.69E-46 |
| MS.gene056083 | MS.gene93506 | 0.816459 | 5.47E-52 | -1.69E-46 |
| MS.gene056166 | MS.gene93506 | 0.860685 | 1.74E-63 | -1.69E-46 |
| MS.gene056374 | MS.gene93506 | 0.848918 | 4.51E-60 | -1.69E-46 |
| MS.gene056387 | MS.gene93506 | 0.803619 | 3.20E-49 | -1.69E-46 |
| MS.gene056487 | MS.gene93506 | 0.838496 | 2.78E-57 | -1.69E-46 |
| MS.gene056504 | MS.gene93506 | 0.863117 | 3.13E-64 | -1.69E-46 |
| MS.gene056510 | MS.gene93506 | 0.851024 | 1.16E-60 | -1.69E-46 |
| MS.gene056511 | MS.gene93506 | 0.804169 | 2.46E-49 | -1.69E-46 |
| MS.gene05664 | MS.gene93506 | 0.808478 | 3.04E-50 | -1.69E-46 |
| MS.gene056657 | MS.gene93506 | 0.875843 | 2.24E-68 | -1.69E-46 |
| MS.gene05666 | MS.gene93506 | 0.829996 | 3.76E-55 | -1.69E-46 |
| MS.gene056692 | MS.gene93506 | 0.812457 | 4.20E-51 | -1.69E-46 |
| MS.gene056942 | MS.gene93506 | 0.803858 | 2.85E-49 | -1.69E-46 |
| MS.gene056946 | MS.gene93506 | 0.807869 | 4.09E-50 | -1.69E-46 |
| MS.gene057048 | MS.gene93506 | 0.833804 | 4.32E-56 | -1.69E-46 |
| MS.gene057076 | MS.gene93506 | 0.853531 | 2.25E-61 | -1.69E-46 |
| MS.gene057086 | MS.gene93506 | 0.846128 | 2.64E-59 | -1.69E-46 |
| MS.gene057124 | MS.gene93506 | 0.816377 | 5.71E-52 | -1.69E-46 |
| MS.gene057129 | MS.gene93506 | 0.8211 | 4.83E-53 | -1.69E-46 |
| MS.gene057146 | MS.gene93506 | 0.809305 | 2.02E-50 | -1.69E-46 |
| MS.gene057416 | MS.gene93506 | 0.857311 | 1.78E-62 | -1.69E-46 |
| MS.gene057658 | MS.gene93506 | 0.831344 | 1.76E-55 | -1.69E-46 |
| MS.gene057734 | MS.gene93506 | 0.828656 | 7.97E-55 | -1.69E-46 |
| MS.gene057749 | MS.gene93506 | 0.802073 | 6.67E-49 | -1.69E-46 |
| MS.gene057823 | MS.gene93506 | 0.804352 | 2.25E-49 | -1.69E-46 |
| MS.gene057831 | MS.gene93506 | 0.855394 | 6.50E-62 | -1.69E-46 |
| MS.gene057856 | MS.gene93506 | 0.824166 | 9.35E-54 | -1.69E-46 |
| MS.gene05787 | MS.gene93506 | 0.822988 | 1.76E-53 | -1.69E-46 |
| MS.gene057926 | MS.gene93506 | 0.81041 | 1.17E-50 | -1.69E-46 |
| MS.gene058019 | MS.gene93506 | 0.845499 | 3.91E-59 | -1.69E-46 |
| MS.gene058130 | MS.gene93506 | 0.850269 | 1.89E-60 | -1.69E-46 |
| MS.gene058157 | MS.gene93506 | 0.80501 | 1.64E-49 | -1.69E-46 |
| MS.gene058334 | MS.gene93506 | -0.821436 | 4.04E-53 | -1.69E-46 |
| MS.gene058338 | MS.gene93506 | 0.818383 | 2.02E-52 | -1.69E-46 |
| MS.gene05837 | MS.gene93506 | 0.820762 | 5.78E-53 | -1.69E-46 |
| MS.gene058419 | MS.gene93506 | 0.874804 | 5.07E-68 | -1.69E-46 |
| MS.gene058544 | MS.gene93506 | 0.857546 | 1.52E-62 | -1.69E-46 |
| MS.gene058723 | MS.gene93506 | 0.816943 | 4.26E-52 | -1.69E-46 |
| MS.gene058773 | MS.gene93506 | 0.815986 | 6.98E-52 | -1.69E-46 |
| MS.gene058838 | MS.gene93506 | 0.854607 | 1.10E-61 | -1.69E-46 |
| MS.gene05883 | MS.gene93506 | 0.837335 | 5.52E-57 | -1.69E-46 |
| MS.gene058928 | MS.gene93506 | 0.802601 | 5.19E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene93506 | 0.856158 | 3.89E-62 | -1.69E-46 |
| MS.gene059023 | MS.gene93506 | 0.85253 | 4.35E-61 | -1.69E-46 |
| MS.gene05913 | MS.gene93506 | 0.812366 | 4.40E-51 | -1.69E-46 |
| MS.gene05914 | MS.gene93506 | 0.807524 | 4.85E-50 | -1.69E-46 |
| MS.gene05934 | MS.gene93506 | 0.833972 | 3.92E-56 | -1.69E-46 |
| MS.gene059367 | MS.gene93506 | 0.867892 | 9.79E-66 | -1.69E-46 |
| MS.gene059368 | MS.gene93506 | 0.842393 | 2.66E-58 | -1.69E-46 |
| MS.gene059455 | MS.gene93506 | 0.835821 | 1.34E-56 | -1.69E-46 |
| MS.gene059526 | MS.gene93506 | -0.80234 | 5.88E-49 | -1.69E-46 |
| MS.gene059536 | MS.gene93506 | 0.832169 | 1.10E-55 | -1.69E-46 |
| MS.gene059667 | MS.gene93506 | 0.803969 | 2.71E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene93506 | 0.869658 | 2.63E-66 | -1.69E-46 |
| MS.gene059718 | MS.gene93506 | 0.888001 | 8.69E-73 | -1.69E-46 |
| MS.gene059756 | MS.gene93506 | 0.818916 | 1.53E-52 | -1.69E-46 |
| MS.gene059801 | MS.gene93506 | 0.812564 | 3.98E-51 | -1.69E-46 |
| MS.gene059865 | MS.gene93506 | 0.864382 | 1.27E-64 | -1.69E-46 |
| MS.gene059902 | MS.gene93506 | 0.815135 | 1.08E-51 | -1.69E-46 |
| MS.gene06000 | MS.gene93506 | 0.823413 | 1.40E-53 | -1.69E-46 |
| MS.gene060482 | MS.gene93506 | 0.858911 | 5.95E-63 | -1.69E-46 |
| MS.gene060485 | MS.gene93506 | 0.81559 | 8.55E-52 | -1.69E-46 |
| MS.gene060703 | MS.gene93506 | 0.837464 | 5.12E-57 | -1.69E-46 |
| MS.gene060762 | MS.gene93506 | 0.866887 | 2.05E-65 | -1.69E-46 |
| MS.gene060778 | MS.gene93506 | 0.871627 | 5.92E-67 | -1.69E-46 |
| MS.gene06077 | MS.gene93506 | 0.855056 | 8.15E-62 | -1.69E-46 |
| MS.gene060804 | MS.gene93506 | 0.827963 | 1.17E-54 | -1.69E-46 |
| MS.gene060835 | MS.gene93506 | 0.856077 | 4.11E-62 | -1.69E-46 |
| MS.gene060837 | MS.gene93506 | 0.816367 | 5.74E-52 | -1.69E-46 |
| MS.gene06092 | MS.gene93506 | 0.832791 | 7.73E-56 | -1.69E-46 |
| MS.gene060982 | MS.gene93506 | 0.862001 | 6.90E-64 | -1.69E-46 |
| MS.gene061041 | MS.gene93506 | 0.832097 | 1.15E-55 | -1.69E-46 |
| MS.gene061134 | MS.gene93506 | 0.805056 | 1.61E-49 | -1.69E-46 |
| MS.gene061150 | MS.gene93506 | 0.836303 | 1.01E-56 | -1.69E-46 |
| MS.gene061200 | MS.gene93506 | 0.8254 | 4.78E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93506.t1 | MTR_3g026020 | 91.411 | 163 | 3 | 2 | 1 | 163 | 38 | 189 | 1.09e-96 | 277 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93506.t1 | AT5G08050 | 62.346 | 162 | 40 | 2 | 1 | 161 | 15 | 156 | 8.60e-50 | 157 |
Find 35 sgRNAs with CRISPR-Local
Find 111 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGTCGAGGTTGGATTAC+AGG | 0.269782 | 3.4:+18238412 | MS.gene93506:CDS |
| GAGACAGAAGGAACTGGTTT+GGG | 0.333662 | 3.4:-18236932 | None:intergenic |
| TGTGTTGTTGGTGGTTCTGC+TGG | 0.362863 | 3.4:+18238452 | MS.gene93506:CDS |
| TAAAACTGTAGCTTCTAAAA+AGG | 0.383526 | 3.4:+18236955 | MS.gene93506:CDS |
| GTTGCTTTGGTCTGTGTTGT+TGG | 0.388658 | 3.4:+18238440 | MS.gene93506:CDS |
| ATTACAGGCTGTGGTTGCTT+TGG | 0.392075 | 3.4:+18238427 | MS.gene93506:CDS |
| AGAGACAGAAGGAACTGGTT+TGG | 0.399810 | 3.4:-18236933 | None:intergenic |
| CATTCTTGTGAGGAGTTTAA+CGG | 0.401167 | 3.4:-18238159 | None:intergenic |
| TAAACCTGCTTTCTCTGCCT+TGG | 0.422974 | 3.4:-18238201 | None:intergenic |
| AACCTCGACAACATTATCTT+CGG | 0.427394 | 3.4:-18238402 | None:intergenic |
| CGTTAAACTCCTCACAAGAA+TGG | 0.466698 | 3.4:+18238160 | MS.gene93506:CDS |
| AAGAGTGATCCTGGAGTTGA+TGG | 0.471176 | 3.4:-18238326 | None:intergenic |
| CTATCAACAATAGAGAAACT+TGG | 0.493062 | 3.4:+18238254 | MS.gene93506:CDS |
| CTGAGTGTGAAGAGTGATCC+TGG | 0.494258 | 3.4:-18238335 | None:intergenic |
| ACCTCGACAACATTATCTTC+GGG | 0.509562 | 3.4:-18238401 | None:intergenic |
| TTATCAGCCGCTGAGAAAGC+AGG | 0.509580 | 3.4:+18238224 | MS.gene93506:CDS |
| GAAGATAATGTTGTCGAGGT+TGG | 0.509847 | 3.4:+18238404 | MS.gene93506:CDS |
| AACTGGTTTGGGAGTAGCCA+TGG | 0.510271 | 3.4:-18236921 | None:intergenic |
| GCTTTGGTCTGTGTTGTTGG+TGG | 0.531295 | 3.4:+18238443 | MS.gene93506:CDS |
| CGAGGTTGGATTACAGGCTG+TGG | 0.533900 | 3.4:+18238418 | MS.gene93506:CDS |
| AATTCAACTGTATTCCCACT+TGG | 0.537612 | 3.4:+18238101 | MS.gene93506:CDS |
| TCCTAGGTTGTTCCCCAAGT+GGG | 0.538069 | 3.4:-18238115 | None:intergenic |
| CTCTCAAAAGCAGAAGAACT+CGG | 0.539080 | 3.4:+18238281 | MS.gene93506:CDS |
| TTAACCAAGGCAGAGAAAGC+AGG | 0.541705 | 3.4:+18238197 | MS.gene93506:CDS |
| GCTACTGATCCATCAACTCC+AGG | 0.551246 | 3.4:+18238317 | MS.gene93506:CDS |
| ATTCAACTGTATTCCCACTT+GGG | 0.553323 | 3.4:+18238102 | MS.gene93506:CDS |
| GTCGATGTTACAGCACTCCT+AGG | 0.557906 | 3.4:-18238131 | None:intergenic |
| TCAGTTGCTCCATTCTTGTG+AGG | 0.565544 | 3.4:-18238169 | None:intergenic |
| TCCCACTTGGGGAACAACCT+AGG | 0.596244 | 3.4:+18238114 | MS.gene93506:CDS |
| TTCAACTGTATTCCCACTTG+GGG | 0.603934 | 3.4:+18238103 | MS.gene93506:CDS |
| TCCCGAAGATAATGTTGTCG+AGG | 0.604575 | 3.4:+18238400 | MS.gene93506:CDS |
| GGAACAAGATAAACAAACAA+AGG | 0.614999 | 3.4:-18238380 | None:intergenic |
| CTCCTAGGTTGTTCCCCAAG+TGG | 0.623775 | 3.4:-18238116 | None:intergenic |
| CGATAATCCTGCTTTCTCAG+CGG | 0.689716 | 3.4:-18238231 | None:intergenic |
| GCAACTGAGACTGTTAACCA+AGG | 0.730927 | 3.4:+18238184 | MS.gene93506:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAGTAAAGTTTCTATGT+AGG | - | chr3.4:18237487-18237506 | None:intergenic | 20.0% |
| !! | ATCCTTTCTCATAATTAAAT+TGG | - | chr3.4:18237074-18237093 | None:intergenic | 20.0% |
| !! | TACCAATTTAATTATGAGAA+AGG | + | chr3.4:18237069-18237088 | MS.gene93506:intron | 20.0% |
| !!! | ATAAACTGTAATTTGTCATA+GGG | + | chr3.4:18237213-18237232 | MS.gene93506:intron | 20.0% |
| !!! | ATATTTGTTTTCCTTTGTTT+TGG | + | chr3.4:18237850-18237869 | MS.gene93506:intron | 20.0% |
| !!! | TTATTTTATTTTCTGCAAGT+TGG | - | chr3.4:18238501-18238520 | None:intergenic | 20.0% |
| ! | ATTACAGTTTATGTATACCT+AGG | - | chr3.4:18237205-18237224 | None:intergenic | 25.0% |
| ! | ATTGAAAACGTAAAATAACC+AGG | - | chr3.4:18237450-18237469 | None:intergenic | 25.0% |
| ! | CATAGAAACTTTACTTTATC+AGG | + | chr3.4:18237488-18237507 | MS.gene93506:intron | 25.0% |
| ! | TATACTGTAAAAAAATCACC+TGG | + | chr3.4:18237429-18237448 | MS.gene93506:intron | 25.0% |
| !! | CATAAACTGTAATTTGTCAT+AGG | + | chr3.4:18237212-18237231 | MS.gene93506:intron | 25.0% |
| !! | TAAAACTGTAGCTTCTAAAA+AGG | + | chr3.4:18236955-18236974 | MS.gene93506:CDS | 25.0% |
| !! | TAAACTGTAATTTGTCATAG+GGG | + | chr3.4:18237214-18237233 | MS.gene93506:intron | 25.0% |
| !! | TGGAATCTTTAGGAAAAAAA+AGG | + | chr3.4:18237403-18237422 | MS.gene93506:intron | 25.0% |
| !! | TTTAATGCTCTTTGATGATA+TGG | + | chr3.4:18237172-18237191 | MS.gene93506:intron | 25.0% |
| !!! | AGCTTTGTTTTACTTCTTTT+AGG | + | chr3.4:18238356-18238375 | MS.gene93506:CDS | 25.0% |
| !!! | TGGAAATGCTTTGTTTAAAT+AGG | - | chr3.4:18237625-18237644 | None:intergenic | 25.0% |
| AATTTACATGATGCCATGTT+TGG | - | chr3.4:18237645-18237664 | None:intergenic | 30.0% | |
| ACCATATGAGTTGTACTTAT+CGG | - | chr3.4:18237288-18237307 | None:intergenic | 30.0% | |
| AGTACAACTCATATGGTAAA+AGG | + | chr3.4:18237291-18237310 | MS.gene93506:intron | 30.0% | |
| ATGGCATCATGTAAATTCTA+CGG | + | chr3.4:18237648-18237667 | MS.gene93506:intron | 30.0% | |
| CCACTTTAATAAACGGTTTA+TGG | - | chr3.4:18237999-18238018 | None:intergenic | 30.0% | |
| CCATAAACCGTTTATTAAAG+TGG | + | chr3.4:18237996-18238015 | MS.gene93506:intron | 30.0% | |
| CTATCAACAATAGAGAAACT+TGG | + | chr3.4:18238254-18238273 | MS.gene93506:CDS | 30.0% | |
| GGAACAAGATAAACAAACAA+AGG | - | chr3.4:18238383-18238402 | None:intergenic | 30.0% | |
| GGTCTGATAAGTACTAAAAA+TGG | + | chr3.4:18237546-18237565 | MS.gene93506:intron | 30.0% | |
| TGTTTGTTCTACCAAAACAA+AGG | - | chr3.4:18237864-18237883 | None:intergenic | 30.0% | |
| ! | AAACAAAGCATTTCCAAACA+TGG | + | chr3.4:18237629-18237648 | MS.gene93506:intron | 30.0% |
| ! | ACAGTCATTGTTATTGTCAT+AGG | - | chr3.4:18237907-18237926 | None:intergenic | 30.0% |
| ! | CTCTTTGATGATATGGTATA+TGG | + | chr3.4:18237179-18237198 | MS.gene93506:intron | 30.0% |
| !! | ACTTTATCAGGCTTTTAAGT+AGG | + | chr3.4:18237500-18237519 | MS.gene93506:intron | 30.0% |
| !! | ATGCTTTGTTTAAATAGGTC+TGG | - | chr3.4:18237620-18237639 | None:intergenic | 30.0% |
| !! | TTTCTACGGTAATTTTCAAG+TGG | + | chr3.4:18237966-18237985 | MS.gene93506:intron | 30.0% |
| AACCTCGACAACATTATCTT+CGG | - | chr3.4:18238405-18238424 | None:intergenic | 35.0% | |
| AATTCAACTGTATTCCCACT+TGG | + | chr3.4:18238101-18238120 | MS.gene93506:CDS | 35.0% | |
| ACAACTCATATGGTAAAAGG+CGG | + | chr3.4:18237294-18237313 | MS.gene93506:intron | 35.0% | |
| ACTAGTTCGAAAAGACATCT+GGG | + | chr3.4:18237803-18237822 | MS.gene93506:intron | 35.0% | |
| ATTCAACTGTATTCCCACTT+GGG | + | chr3.4:18238102-18238121 | MS.gene93506:CDS | 35.0% | |
| CATTCTTGTGAGGAGTTTAA+CGG | - | chr3.4:18238162-18238181 | None:intergenic | 35.0% | |
| CGGTAGACCACTTTAATAAA+CGG | - | chr3.4:18238006-18238025 | None:intergenic | 35.0% | |
| TAACCTGAAATTACGACTCA+TGG | + | chr3.4:18237715-18237734 | MS.gene93506:intron | 35.0% | |
| TACTAGTTCGAAAAGACATC+TGG | + | chr3.4:18237802-18237821 | MS.gene93506:intron | 35.0% | |
| TATTGTCATAGGTGATACAG+TGG | - | chr3.4:18237896-18237915 | None:intergenic | 35.0% | |
| TCCGATAAGTACAACTCATA+TGG | + | chr3.4:18237284-18237303 | MS.gene93506:intron | 35.0% | |
| ! | AACTTTTGTCCCCAAAAAAC+AGG | - | chr3.4:18238038-18238057 | None:intergenic | 35.0% |
| ! | TACTTATCAGACCTTTTCGA+AGG | - | chr3.4:18237539-18237558 | None:intergenic | 35.0% |
| AATACGTCAGACCTTCGAAA+AGG | + | chr3.4:18237525-18237544 | MS.gene93506:intron | 40.0% | |
| ACATTTGATATGCTGAGACG+CGG | + | chr3.4:18237322-18237341 | MS.gene93506:intron | 40.0% | |
| ACCTCGACAACATTATCTTC+GGG | - | chr3.4:18238404-18238423 | None:intergenic | 40.0% | |
| CGTTAAACTCCTCACAAGAA+TGG | + | chr3.4:18238160-18238179 | MS.gene93506:CDS | 40.0% | |
| CTCTCAAAAGCAGAAGAACT+CGG | + | chr3.4:18238281-18238300 | MS.gene93506:CDS | 40.0% | |
| CTCTGTCTTAATGAGTGAGA+CGG | + | chr3.4:18237767-18237786 | MS.gene93506:intron | 40.0% | |
| GAAGATAATGTTGTCGAGGT+TGG | + | chr3.4:18238404-18238423 | MS.gene93506:CDS | 40.0% | |
| GATGATATGGTATATGGCCT+AGG | + | chr3.4:18237185-18237204 | MS.gene93506:intron | 40.0% | |
| GCATCATGTAAATTCTACGG+TGG | + | chr3.4:18237651-18237670 | MS.gene93506:intron | 40.0% | |
| GTACCATGAGTCGTAATTTC+AGG | - | chr3.4:18237721-18237740 | None:intergenic | 40.0% | |
| TTCAACTGTATTCCCACTTG+GGG | + | chr3.4:18238103-18238122 | MS.gene93506:CDS | 40.0% | |
| TTCTACTTCCAAGAGTGACA+TGG | + | chr3.4:18237931-18237950 | MS.gene93506:intron | 40.0% | |
| ! | GTTTTGTCGCTGGAATCTTT+AGG | + | chr3.4:18237393-18237412 | MS.gene93506:intron | 40.0% |
| ! | TTTCAAGTGTTAATCCACCG+TGG | - | chr3.4:18237694-18237713 | None:intergenic | 40.0% |
| !! | CAAAAAACAGGCTGGTTCTA+CGG | - | chr3.4:18238026-18238045 | None:intergenic | 40.0% |
| !!! | CTACGGTAATTTTCAAGTGG+TGG | + | chr3.4:18237969-18237988 | MS.gene93506:intron | 40.0% |
| !!! | GCTACAGTTTTAGAGACAGA+AGG | - | chr3.4:18236947-18236966 | None:intergenic | 40.0% |
| !!! | GTAGAACCAGCCTGTTTTTT+GGG | + | chr3.4:18238025-18238044 | MS.gene93506:intron | 40.0% |
| !!! | GTTTTAGAGACAGAAGGAAC+TGG | - | chr3.4:18236941-18236960 | None:intergenic | 40.0% |
| !!! | TAGAACCAGCCTGTTTTTTG+GGG | + | chr3.4:18238026-18238045 | MS.gene93506:intron | 40.0% |
| AAGAGTGACATGGTTGTCGT+CGG | + | chr3.4:18237941-18237960 | MS.gene93506:intron | 45.0% | |
| AAGAGTGATCCTGGAGTTGA+TGG | - | chr3.4:18238329-18238348 | None:intergenic | 45.0% | |
| ACTCATATGGTAAAAGGCGG+AGG | + | chr3.4:18237297-18237316 | MS.gene93506:intron | 45.0% | |
| AGAGACAGAAGGAACTGGTT+TGG | - | chr3.4:18236936-18236955 | None:intergenic | 45.0% | |
| ATTACAGGCTGTGGTTGCTT+TGG | + | chr3.4:18238427-18238446 | MS.gene93506:CDS | 45.0% | |
| CATTTGATATGCTGAGACGC+GGG | + | chr3.4:18237323-18237342 | MS.gene93506:intron | 45.0% | |
| CGACAAAACTCACACTCACA+CGG | - | chr3.4:18237382-18237401 | None:intergenic | 45.0% | |
| CGATAATCCTGCTTTCTCAG+CGG | - | chr3.4:18238234-18238253 | None:intergenic | 45.0% | |
| CGTCTCACTCATTAAGACAG+AGG | - | chr3.4:18237769-18237788 | None:intergenic | 45.0% | |
| CTCATATGGTAAAAGGCGGA+GGG | + | chr3.4:18237298-18237317 | MS.gene93506:intron | 45.0% | |
| GCAACTGAGACTGTTAACCA+AGG | + | chr3.4:18238184-18238203 | MS.gene93506:CDS | 45.0% | |
| TAAACCTGCTTTCTCTGCCT+TGG | - | chr3.4:18238204-18238223 | None:intergenic | 45.0% | |
| TCAGTTGCTCCATTCTTGTG+AGG | - | chr3.4:18238172-18238191 | None:intergenic | 45.0% | |
| TCATATGGTAAAAGGCGGAG+GGG | + | chr3.4:18237299-18237318 | MS.gene93506:intron | 45.0% | |
| TCCCGAAGATAATGTTGTCG+AGG | + | chr3.4:18238400-18238419 | MS.gene93506:CDS | 45.0% | |
| TGTTGTCGAGGTTGGATTAC+AGG | + | chr3.4:18238412-18238431 | MS.gene93506:CDS | 45.0% | |
| TTAACCAAGGCAGAGAAAGC+AGG | + | chr3.4:18238197-18238216 | MS.gene93506:CDS | 45.0% | |
| TTTGTCCCCAAAAAACAGGC+TGG | - | chr3.4:18238034-18238053 | None:intergenic | 45.0% | |
| ! | ACACGGACGAAGTGTTAAGT+AGG | - | chr3.4:18237365-18237384 | None:intergenic | 45.0% |
| ! | CACGGACGAAGTGTTAAGTA+GGG | - | chr3.4:18237364-18237383 | None:intergenic | 45.0% |
| ! | GAGACAGAAGGAACTGGTTT+GGG | - | chr3.4:18236935-18236954 | None:intergenic | 45.0% |
| ! | GGTTGTCGTCGGTATTTCTA+CGG | + | chr3.4:18237952-18237971 | MS.gene93506:intron | 45.0% |
| ! | GTTGCTTTGGTCTGTGTTGT+TGG | + | chr3.4:18238440-18238459 | MS.gene93506:CDS | 45.0% |
| !! | CGAGTTCTTCTGCTTTTGAG+AGG | - | chr3.4:18238283-18238302 | None:intergenic | 45.0% |
| !!! | CGTAGAACCAGCCTGTTTTT+TGG | + | chr3.4:18238024-18238043 | MS.gene93506:intron | 45.0% |
| AAATTCTACGGTGGTCCACC+TGG | + | chr3.4:18237660-18237679 | MS.gene93506:intron | 50.0% | |
| CTGAGTGTGAAGAGTGATCC+TGG | - | chr3.4:18238338-18238357 | None:intergenic | 50.0% | |
| GACGACAACCATGTCACTCT+TGG | - | chr3.4:18237942-18237961 | None:intergenic | 50.0% | |
| GCTACTGATCCATCAACTCC+AGG | + | chr3.4:18238317-18238336 | MS.gene93506:CDS | 50.0% | |
| GTCGATGTTACAGCACTCCT+AGG | - | chr3.4:18238134-18238153 | None:intergenic | 50.0% | |
| TCCTAGGTTGTTCCCCAAGT+GGG | - | chr3.4:18238118-18238137 | None:intergenic | 50.0% | |
| TTATCAGCCGCTGAGAAAGC+AGG | + | chr3.4:18238224-18238243 | MS.gene93506:CDS | 50.0% | |
| ! | GCTTTGGTCTGTGTTGTTGG+TGG | + | chr3.4:18238443-18238462 | MS.gene93506:CDS | 50.0% |
| !! | GTGAGTGTGAGTTTTGTCGC+TGG | + | chr3.4:18237383-18237402 | MS.gene93506:intron | 50.0% |
| !! | TGTGTTGTTGGTGGTTCTGC+TGG | + | chr3.4:18238452-18238471 | MS.gene93506:CDS | 50.0% |
| CGAGGTTGGATTACAGGCTG+TGG | + | chr3.4:18238418-18238437 | MS.gene93506:CDS | 55.0% | |
| CTCCTAGGTTGTTCCCCAAG+TGG | - | chr3.4:18238119-18238138 | None:intergenic | 55.0% | |
| GAGTGAGACGGTGACTCGAT+TGG | + | chr3.4:18237779-18237798 | MS.gene93506:intron | 55.0% | |
| TCCACCTGGATAGGTGTCCA+CGG | + | chr3.4:18237674-18237693 | MS.gene93506:intron | 55.0% | |
| TCCCACTTGGGGAACAACCT+AGG | + | chr3.4:18238114-18238133 | MS.gene93506:CDS | 55.0% | |
| !!! | GGTGGTTCTGCTGGTTTTGC+TGG | + | chr3.4:18238461-18238480 | MS.gene93506:CDS | 55.0% |
| ACCGTGGACACCTATCCAGG+TGG | - | chr3.4:18237678-18237697 | None:intergenic | 60.0% | |
| ACCTGGATAGGTGTCCACGG+TGG | + | chr3.4:18237677-18237696 | MS.gene93506:intron | 60.0% | |
| CTACGGTGGTCCACCTGGAT+AGG | + | chr3.4:18237665-18237684 | MS.gene93506:intron | 60.0% | |
| TCCACCGTGGACACCTATCC+AGG | - | chr3.4:18237681-18237700 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 18236923 | 18238520 | 18236923 | ID=MS.gene93506 |
| chr3.4 | mRNA | 18236923 | 18238520 | 18236923 | ID=MS.gene93506.t1;Parent=MS.gene93506 |
| chr3.4 | exon | 18236923 | 18236976 | 18236923 | ID=MS.gene93506.t1.exon1;Parent=MS.gene93506.t1 |
| chr3.4 | CDS | 18236923 | 18236976 | 18236923 | ID=cds.MS.gene93506.t1;Parent=MS.gene93506.t1 |
| chr3.4 | exon | 18238083 | 18238520 | 18238083 | ID=MS.gene93506.t1.exon2;Parent=MS.gene93506.t1 |
| chr3.4 | CDS | 18238083 | 18238520 | 18238083 | ID=cds.MS.gene93506.t1;Parent=MS.gene93506.t1 |
| Gene Sequence |
| Protein sequence |
