AlfalfaGEDB | Msa0247550

M. sativa cultivar zhongmu-4 / Msa0247550

KEGG: K11878
GO: -
PFAM: PF16093.8

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0247550	XP_013463686.1	96.479	142	5	0	1	142	1	142	4.89e-96	283

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0247550	A0A072V7V5	96.479	142	5	0	1	142	1	142	2.33e-96	283

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003890	Msa0247550	0.804618	1.981159e-49	-8.615850e-47
Msa0011960	Msa0247550	0.825701	4.059877e-54	-8.615850e-47
Msa0020410	Msa0247550	0.803945	2.736134e-49	-8.615850e-47
Msa0025730	Msa0247550	0.813419	2.584282e-51	-8.615850e-47
Msa0027260	Msa0247550	0.819809	9.555229e-53	-8.615850e-47
Msa0027770	Msa0247550	0.801534	8.606426e-49	-8.615850e-47
Msa0028660	Msa0247550	0.804990	1.656611e-49	-8.615850e-47
Msa0032580	Msa0247550	0.830743	2.473267e-55	-8.615850e-47
Msa0043660	Msa0247550	0.812844	3.455474e-51	-8.615850e-47
Msa0054440	Msa0247550	0.811618	6.399446e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0247550	MtrunA17_Chr2g0302621	96.479	142	5	0	1	142	1	142	4.49e-100	283

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0247550	AT1G48170.1	70.492	122	34	1	19	138	28	149	6.64e-61	184

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Find 46 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGATTATCCTTCCAATCT+TGG	0.292195	2_2:+29961178	None:intergenic
AAATGAAAATTACTATCAAA+AGG	0.485783	2_2:+29960873	None:intergenic
AAGGATAATCTTTGTAGCAT+TGG	0.434566	2_2:-29961168	Msa0247550:exon
AATTCTGAAGAATTAGGAAA+AGG	0.448685	2_2:-29963997	Msa0247550:CDS
AATTCTGATAATACAGGTTC+TGG	0.344534	2_2:-29963155	Msa0247550:CDS
ACAAGAAATTCACACATCAA+AGG	0.589968	2_2:+29961014	None:intergenic
ACATTGGGATAATACATGTT+CGG	0.533538	2_2:+29961123	None:intergenic
AGAGAGCGTACCTCAACCAT+AGG	0.610302	2_2:+29962974	None:intergenic
AGATAGATATATGTGTGGAT+TGG	0.621609	2_2:-29963738	Msa0247550:intron
AGCGAATAATCTGAAAATGG+AGG	0.664738	2_2:+29963887	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAAGCGAATAATCTGAAAA+TGG	+	chr2_2:29961030-29961049	None:intergenic	30.0%
AGAAATTAGGGTTTGAAAAG+GGG	+	chr2_2:29961067-29961086	None:intergenic	30.0%
GAAGAAATTAGGGTTTGAAA+AGG	+	chr2_2:29961069-29961088	None:intergenic	30.0%
AAACCCTAATTTCTTCAACT+GGG	-	chr2_2:29961073-29961092	Msa0247550:three_prime_UTR	30.0%
GTGATAGATAGATATATGTG+TGG	-	chr2_2:29961168-29961187	Msa0247550:exon	30.0%
AGATAGATATATGTGTGGAT+TGG	-	chr2_2:29961173-29961192	Msa0247550:exon	30.0%
AGATTATGCATGGTTATATC+CGG	-	chr2_2:29961614-29961633	Msa0247550:intron	30.0%
TTCAGTGCTACATTAATAGA+TGG	-	chr2_2:29961666-29961685	Msa0247550:intron	30.0%
TTAATACTTGCAGAACAATG+TGG	-	chr2_2:29961707-29961726	Msa0247550:intron	30.0%
GTTTCTGTAACTTCTATACT+TGG	-	chr2_2:29961729-29961748	Msa0247550:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	29960860	29964073	29960860	ID=Msa0247550;Name=Msa0247550
chr2_2	mRNA	29960860	29964073	29960860	ID=Msa0247550-mRNA-1;Parent=Msa0247550;Name=Msa0247550-mRNA-1;_AED=0.02;_eAED=0.02;_QI=51\|1\|1\|1\|1\|1\|5\|323\|142
chr2_2	exon	29960860	29961254	29960860	ID=Msa0247550-mRNA-1:exon:10603;Parent=Msa0247550-mRNA-1
chr2_2	exon	29962985	29963049	29962985	ID=Msa0247550-mRNA-1:exon:10602;Parent=Msa0247550-mRNA-1
chr2_2	exon	29963138	29963213	29963138	ID=Msa0247550-mRNA-1:exon:10601;Parent=Msa0247550-mRNA-1
chr2_2	exon	29963686	29963754	29963686	ID=Msa0247550-mRNA-1:exon:10600;Parent=Msa0247550-mRNA-1
chr2_2	exon	29963876	29964073	29963876	ID=Msa0247550-mRNA-1:exon:10599;Parent=Msa0247550-mRNA-1
chr2_2	five_prime_UTR	29964023	29964073	29964023	ID=Msa0247550-mRNA-1:five_prime_utr;Parent=Msa0247550-mRNA-1
chr2_2	CDS	29963876	29964022	29963876	ID=Msa0247550-mRNA-1:cds;Parent=Msa0247550-mRNA-1
chr2_2	CDS	29963686	29963754	29963686	ID=Msa0247550-mRNA-1:cds;Parent=Msa0247550-mRNA-1
chr2_2	CDS	29963138	29963213	29963138	ID=Msa0247550-mRNA-1:cds;Parent=Msa0247550-mRNA-1
chr2_2	CDS	29962985	29963049	29962985	ID=Msa0247550-mRNA-1:cds;Parent=Msa0247550-mRNA-1
chr2_2	CDS	29961183	29961254	29961183	ID=Msa0247550-mRNA-1:cds;Parent=Msa0247550-mRNA-1
chr2_2	three_prime_UTR	29960860	29961182	29960860	ID=Msa0247550-mRNA-1:three_prime_utr;Parent=Msa0247550-mRNA-1

Gene Sequence

>Msa0247550

ATGAATTCTGAAGAATTAGGAAAAGGGATTCAAGAATTGAAACTCAATCATAAAGAAGAAGAAGAGAGTCAATTGCAATTCACTTGCTTCACTGAACTTGTCAACGATGTTAACCTCCATTTTCAGATTATTCGCTTTCCCAAACAGATATATGTGTGGATTGGTTACAACTCTGCAAAATTGGGACAACTTTATGCAGCAGCTTCAACTCGTCCTAACAATGTGGTTTCTGTAACTTCTATACTTGGGGGGAATTCTGATAATACAGGTTCTGGCATTGCTCGTCGATTAGTGTTGAAGACTGGTCTCAATATAATCATGGCTTGCAATATTCCAAAAAATAGTCCTATGGTTGAGATAGAAGCCGAGAAAATACTGATACAAAAACTTATTAGTTTGGGCTACACCAAGCCAAGATTGGAAGGATAA

Protein sequence

>Msa0247550

MNSEELGKGIQELKLNHKEEEESQLQFTCFTELVNDVNLHFQIIRFPKQIYVWIGYNSAKLGQLYAAASTRPNNVVSVTSILGGNSDNTGSGIARRLVLKTGLNIIMACNIPKNSPMVEIEAEKILIQKLISLGYTKPRLEG*