AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1228580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1228580 MtrunA17_Chr8g0335341 95.336 729 27 2 1 728 1 723 0.0 1383
Msa1228580 MtrunA17_Chr7g0250681 68.550 531 128 7 227 728 323 843 0.0 704
Msa1228580 MtrunA17_Chr5g0405741 39.341 455 241 9 282 724 204 635 4.08e-92 300
Msa1228580 MtrunA17_Chr2g0303521 31.195 452 276 8 282 724 208 633 3.24e-52 192
Msa1228580 MtrunA17_Chr3g0139411 28.667 450 282 11 284 720 277 700 3.85e-51 190
Msa1228580 MtrunA17_Chr2g0331821 28.507 442 299 9 274 710 309 738 8.10e-48 181
Msa1228580 MtrunA17_Chr2g0331821 57.627 59 25 0 1 59 1 59 1.92e-14 77.4
Msa1228580 MtrunA17_Chr3g0124841 27.152 453 309 7 282 720 454 899 1.50e-43 169
Msa1228580 MtrunA17_Chr4g0004471 31.222 442 274 11 275 710 119 536 8.25e-43 163
Msa1228580 MtrunA17_Chr1g0211271 28.027 446 294 8 282 710 274 709 1.31e-41 162
Msa1228580 MtrunA17_Chr1g0154051 79.048 105 17 2 34 138 32 131 7.16e-39 140
Msa1228580 MtrunA17_Chr5g0448571 23.394 436 291 9 286 716 358 755 4.35e-27 117
Msa1228580 MtrunA17_Chr4g0072011 27.059 340 227 7 277 602 330 662 8.62e-27 116
Msa1228580 MtrunA17_Chr3g0143731 25.828 453 256 12 282 711 275 670 3.32e-26 114
Msa1228580 MtrunA17_Chr8g0341331 73.077 104 20 3 31 131 27 125 3.36e-25 102
Msa1228580 MtrunA17_Chr5g0440551 33.621 232 137 5 366 588 429 652 7.90e-25 110
Msa1228580 MtrunA17_Chr2g0298171 39.106 179 97 4 369 537 628 804 3.84e-24 108
Msa1228580 MtrunA17_Chr3g0130761 27.246 345 237 6 367 710 433 764 4.64e-24 108
Msa1228580 MtrunA17_Chr3g0118401 32.751 229 147 3 366 590 445 670 4.59e-23 104
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1228580 AT1G02110.1 57.296 747 254 17 1 727 1 702 0.0 795
Msa1228580 AT3G60320.1 56.089 813 255 18 1 728 1 796 0.0 775
Msa1228580 AT3G51290.3 40.889 450 241 9 282 727 200 628 9.13e-95 308
Msa1228580 AT3G51290.2 41.111 450 240 9 282 727 200 628 4.62e-93 307
Msa1228580 AT3G51290.1 40.444 450 237 10 282 727 200 622 4.63e-89 292
Msa1228580 AT2G34670.2 34.332 367 224 6 355 720 324 674 6.62e-60 215
Msa1228580 AT5G25590.1 29.386 456 277 9 283 720 330 758 2.87e-52 194
Msa1228580 AT1G52320.3 29.078 423 279 9 293 710 34 440 9.93e-50 181
Msa1228580 AT1G52320.4 29.078 423 279 9 293 710 34 440 9.93e-50 181
Msa1228580 AT1G52320.1 29.078 423 279 9 293 710 337 743 6.36e-49 184
Msa1228580 AT1G52320.2 29.078 423 279 9 293 710 360 766 9.06e-49 184
Msa1228580 AT1G52320.5 29.078 423 279 9 293 710 360 766 9.06e-49 184
Msa1228580 AT1G21740.1 28.667 450 305 8 282 720 497 941 5.87e-45 174
Msa1228580 AT1G77500.1 27.434 452 285 9 282 720 449 870 4.69e-39 155
Msa1228580 AT2G34670.1 33.628 226 142 4 355 580 324 541 3.20e-32 132
Msa1228580 AT4G35240.4 24.777 448 297 7 282 724 419 831 1.30e-29 126
Msa1228580 AT4G35240.3 24.777 448 297 7 282 724 419 831 1.30e-29 126
Msa1228580 AT4G35240.2 24.777 448 297 7 282 724 419 831 1.76e-29 125
Msa1228580 AT4G35240.1 24.777 448 297 7 282 724 419 831 1.76e-29 125
Msa1228580 AT2G17110.1 29.321 324 208 8 286 594 313 630 8.15e-29 123
Msa1228580 AT2G27090.2 33.621 232 144 6 366 592 425 651 8.65e-25 110
Msa1228580 AT2G27090.1 33.621 232 144 6 366 592 425 651 8.65e-25 110
Msa1228580 AT5G54480.1 25.862 406 244 11 247 626 270 644 9.52e-24 107
Msa1228580 AT1G20530.1 26.546 388 246 10 283 654 232 596 1.90e-23 105
Msa1228580 AT4G39790.1 31.696 224 146 5 369 588 375 595 2.35e-22 102
Msa1228580 AT4G30130.3 31.429 245 153 5 369 602 401 641 1.00e-21 100
Msa1228580 AT4G30130.1 31.429 245 153 5 369 602 401 641 1.00e-21 100
Msa1228580 AT2G19090.1 30.364 247 155 5 369 602 482 724 2.88e-21 99.8
Msa1228580 AT4G30130.4 43.210 81 45 1 369 449 401 480 9.19e-12 68.9
Msa1228580 AT4G30130.2 43.210 81 45 1 369 449 401 480 9.19e-12 68.9

Find 183 sgRNAs with CRISPR-Local

Find 307 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATAAATTACCTACTTTA+TGG 0.098163 8_2:-4020019 None:intergenic
TGCCGGAAATATTCGGAATT+TGG 0.192821 8_2:-4016160 None:intergenic
CTCCACTTTGGACCGGCTTT+TGG 0.216350 8_2:+4016989 Msa1228580:CDS
CAGCTGCCATCAATGGTCTT+AGG 0.218432 8_2:+4018005 Msa1228580:CDS
TCCCCTCTTATAGGAAATTA+AGG 0.220886 8_2:+4020172 Msa1228580:three_prime_UTR
CTCCAAATTCCGAATATTTC+CGG 0.231370 8_2:+4016158 Msa1228580:CDS
TCTGATGATGTATCGGATTC+TGG 0.247422 8_2:-4016217 None:intergenic
CCAATATCTTCCACAGAGTT+TGG 0.255194 8_2:-4019769 None:intergenic
GCTGTGCAACCCATTGTTTC+AGG 0.258271 8_2:-4015674 None:intergenic
GTCAAGAACTCAAGATTAAA+AGG 0.266491 8_2:+4019641 Msa1228580:CDS
CCAAACTCTGTGGAAGATAT+TGG 0.303854 8_2:+4019769 Msa1228580:CDS
ATGGGAAAACAAACTCTTTC+AGG 0.306558 8_2:+4017013 Msa1228580:CDS
AAACTTCTTCCGTTTGGAAC+TGG 0.310325 8_2:+4016110 Msa1228580:CDS
AGGTTTGCCTGGAATGTTTC+AGG 0.312214 8_2:+4019933 Msa1228580:CDS
CGGAGATGTTGCAGATTAGT+AGG 0.332632 8_2:+4016599 Msa1228580:CDS
AGAACGTTGCCGGAAATATT+CGG 0.340163 8_2:-4016167 None:intergenic
TCTCCTTAATTTCCTATAAG+AGG 0.341283 8_2:-4020175 None:intergenic
TCATATTCTATGGTTGGTAT+TGG 0.351215 8_2:+4019739 Msa1228580:CDS
CAATGTTGCTTTGCGTTTCA+TGG 0.352365 8_2:-4019261 None:intergenic
AAAGTGGAGCAGAGGCTCTT+TGG 0.352489 8_2:-4016975 None:intergenic
TGTTGCCGGCGGTGAGGTTC+CGG 0.353203 8_2:+4016456 Msa1228580:CDS
ATAATTAATCTGTTCTGCTT+TGG 0.354765 8_2:+4020037 Msa1228580:three_prime_UTR
CAGCAGCATTAGCAACAAAA+TGG 0.360224 8_2:+4019492 Msa1228580:CDS
TCACCGCCGGCAACAAATTC+CGG 0.362313 8_2:-4016448 None:intergenic
GCTTCCGAAGCGACAGTGTC+TGG 0.370101 8_2:-4019571 None:intergenic
CTTCTGAAACTTCTTCCGTT+TGG 0.372515 8_2:+4016104 Msa1228580:CDS
TTTGTGGTTGTTACGGTGGC+TGG 0.373103 8_2:-4015938 None:intergenic
TGCTTTGCGTTTCATGGTAC+TGG 0.378095 8_2:-4019255 None:intergenic
CTGTTCTGCTTTGGCTTTCG+AGG 0.382977 8_2:+4020046 Msa1228580:three_prime_UTR
AAATAGAATCACAAAATTCT+TGG 0.384792 8_2:-4020149 None:intergenic
TTATAGGAAATTAAGGAGAT+TGG 0.393221 8_2:+4020179 Msa1228580:three_prime_UTR
AACTGCCAATGGTGGCTTTG+AGG 0.395022 8_2:-4016910 None:intergenic
TTTGATGCCGGTTCACTTGA+AGG 0.399037 8_2:+4016942 Msa1228580:CDS
TTAATCTGAAGGACTAGGTT+AGG 0.406705 8_2:-4015522 None:intergenic
TAGTGACTGTAACCTTGTTA+TGG 0.407255 8_2:-4017948 None:intergenic
AGGAGGTTCGGTGTAGTGAT+TGG 0.415100 8_2:+4016350 Msa1228580:CDS
GTCAATGCCTGAAACATTCC+AGG 0.419912 8_2:-4019940 None:intergenic
TCGGCGGTTGAGAACGTTGC+CGG 0.420345 8_2:-4016177 None:intergenic
AGCTGCCATCAATGGTCTTA+GGG 0.421008 8_2:+4018006 Msa1228580:CDS
CAGCTTGTTGAGCTTTGTCA+TGG 0.421587 8_2:+4018046 Msa1228580:CDS
AGTTGCCTGTTTGTGTGATT+CGG 0.424981 8_2:-4019335 None:intergenic
TGTTCTGCTTTGGCTTTCGA+GGG 0.426961 8_2:+4020047 Msa1228580:three_prime_UTR
GCCCGGTCCTTCAAGTGAAC+CGG 0.428595 8_2:-4016949 None:intergenic
AACTTCTTCCGTTTGGAACT+GGG 0.429013 8_2:+4016111 Msa1228580:CDS
AGAGTCCCTAAGACCATTGA+TGG 0.436049 8_2:-4018011 None:intergenic
TAACCTTGTTATGGTAGCCT+TGG 0.439375 8_2:-4017939 None:intergenic
AACATCCGAATCACACAAAC+AGG 0.442893 8_2:+4019330 Msa1228580:CDS
AACATTGTACAGCAAGTTTG+TGG 0.443164 8_2:+4019277 Msa1228580:CDS
AAATCACCGGAATTTGTTGC+CGG 0.450095 8_2:+4016442 Msa1228580:CDS
AATCTACAGACAGGTTTGCC+TGG 0.453101 8_2:+4019922 Msa1228580:CDS
TTAGAACTTGGAACAATAAG+AGG 0.456298 8_2:-4020109 None:intergenic
GAAGGTGGAGAGTGCGGAAC+CGG 0.456738 8_2:-4015825 None:intergenic
ACGGTGGCTGGAGGAGGAGG+TGG 0.456906 8_2:-4015926 None:intergenic
ACAACAAAATAGTTAGAACT+TGG 0.457524 8_2:-4020121 None:intergenic
GTTGTTACGGTGGCTGGAGG+AGG 0.459155 8_2:-4015932 None:intergenic
ATATATACCACCTGAAACAA+TGG 0.459754 8_2:+4015664 Msa1228580:exon
GTGGCTGGAGGAGGAGGTGG+CGG 0.461153 8_2:-4015923 None:intergenic
CACCATTGGCAGTTAAATAC+CGG 0.462465 8_2:+4016919 Msa1228580:CDS
CTCCTTAATTTCCTATAAGA+GGG 0.463804 8_2:-4020174 None:intergenic
TTGTTGTTGTCATTGTTGAC+TGG 0.464323 8_2:-4019469 None:intergenic
CGAGGAGGTGAATGAAGAAA+AGG 0.465133 8_2:+4016489 Msa1228580:CDS
GTTACGGTGGCTGGAGGAGG+AGG 0.465245 8_2:-4015929 None:intergenic
TATTCGGAATTTGGAGGAGA+AGG 0.467016 8_2:-4016151 None:intergenic
GGAGGTTCGGTGTAGTGATT+GGG 0.467726 8_2:+4016351 Msa1228580:CDS
TGCCGGTTCACTTGAAGGAC+CGG 0.468386 8_2:+4016947 Msa1228580:CDS
ACTAATTATTGTAACATCTC+AGG 0.470791 8_2:+4017967 Msa1228580:CDS
GTTAAATACCGGTTTGATGC+CGG 0.472409 8_2:+4016930 Msa1228580:CDS
CAGTTCGGAAACTCGCTTCT+CGG 0.476259 8_2:-4016046 None:intergenic
TCGGAATTTGGAGGAGAAGG+AGG 0.477917 8_2:-4016148 None:intergenic
GAAAACTGACACTGCTTACA+AGG 0.482447 8_2:+4019663 Msa1228580:CDS
TACAACTCATATTCTATGGT+TGG 0.486108 8_2:+4019733 Msa1228580:CDS
AAAGGTGAAGATGAAGCATA+AGG 0.487575 8_2:+4016507 Msa1228580:CDS
AAACGGAACGTGAGGAGGTT+CGG 0.488757 8_2:+4016338 Msa1228580:CDS
AAAGAGCCTCTGCTCCACTT+TGG 0.490480 8_2:+4016977 Msa1228580:CDS
CGTTGAACAAACGCTTGAAT+CGG 0.491262 8_2:-4016023 None:intergenic
TTTGAGAAAGCGGCGGTGGC+CGG 0.491434 8_2:+4016565 Msa1228580:CDS
GAATTTGGAGGAGAAGGAGG+AGG 0.496035 8_2:-4016145 None:intergenic
TGTTCCAGACACTGTCGCTT+CGG 0.496334 8_2:+4019567 Msa1228580:CDS
ACCTCCTCGTCTTTCCTCAC+CGG 0.497977 8_2:-4016475 None:intergenic
ACTACCTCAGCTGCCATCAA+TGG 0.500376 8_2:+4017998 Msa1228580:CDS
ACACAGACAAGTGGAGGAGC+CGG 0.503009 8_2:+4020249 Msa1228580:three_prime_UTR
GAAGCATTCGAAGGCTATTG+AGG 0.506274 8_2:+4019876 Msa1228580:CDS
GCACTCTCCACCTTCGCCGC+CGG 0.510838 8_2:+4015833 Msa1228580:CDS
GTGTGAAGATTGAGCATGAA+AGG 0.511307 8_2:+4017852 Msa1228580:CDS
AGGTTCACCGGCGGCGAAGG+TGG 0.514217 8_2:-4015840 None:intergenic
AGAATGTGTGGAAGCTTCAC+CGG 0.514510 8_2:-4015998 None:intergenic
TAGTTTCCATCCATCACAAA+AGG 0.515874 8_2:-4019533 None:intergenic
CGGAAATATTCGGAATTTGG+AGG 0.517284 8_2:-4016157 None:intergenic
GGTGGCCGGAGATCGGTTAT+CGG 0.518645 8_2:+4016579 Msa1228580:CDS
GAGAGGTTCACCGGCGGCGA+AGG 0.519230 8_2:-4015843 None:intergenic
TATATACCACCTGAAACAAT+GGG 0.521293 8_2:+4015665 Msa1228580:exon
CTTGTGAACCAATCATCAAA+AGG 0.523864 8_2:+4019301 Msa1228580:CDS
GGAACAAGAACTAGATCGAT+TGG 0.523973 8_2:+4016315 Msa1228580:CDS
AGCTTGTTGAGCTTTGTCAT+GGG 0.524450 8_2:+4018047 Msa1228580:CDS
CGAGAAGCGAGTTTCCGAAC+TGG 0.524694 8_2:+4016047 Msa1228580:CDS
TAGAATCAGCTCTGTCAGCT+TGG 0.525936 8_2:+4019365 Msa1228580:CDS
GGGGTGTGAGTTCAGTATAA+AGG 0.528014 8_2:+4020067 Msa1228580:three_prime_UTR
CAAGTGAACCGGCATCAAAC+CGG 0.530021 8_2:-4016938 None:intergenic
TGAGCAAAGTGAGCTCAACT+TGG 0.530284 8_2:+4016886 Msa1228580:CDS
AAGCGGCGGTGGCCGGAGAT+CGG 0.531676 8_2:+4016572 Msa1228580:CDS
CAGAGGCTCTTTGGACCGCC+CGG 0.533714 8_2:-4016966 None:intergenic
CACAGACAAGTGGAGGAGCC+GGG 0.533810 8_2:+4020250 Msa1228580:three_prime_UTR
GGAAGATATTGGACAAGGCT+TGG 0.535265 8_2:+4019780 Msa1228580:CDS
AGAACTAGATCGATTGGAAA+CGG 0.539459 8_2:+4016321 Msa1228580:CDS
TCAGAATCACAAGACTGAAA+CGG 0.540629 8_2:+4016294 Msa1228580:CDS
TCAACCGCCGAATCATAAAC+CGG 0.541451 8_2:+4016189 Msa1228580:CDS
AAGTGTTAATCTGAAGGACT+AGG 0.544611 8_2:-4015527 None:intergenic
ACACTTAATAATCTACAGAC+AGG 0.546583 8_2:+4019913 Msa1228580:CDS
GCCTCTGCTCCACTTTGGAC+CGG 0.546977 8_2:+4016982 Msa1228580:CDS
TTGCTTAATTAGGCTAGAGA+AGG 0.551085 8_2:+4017830 Msa1228580:intron
ATTGGAAACGGAACGTGAGG+AGG 0.556480 8_2:+4016333 Msa1228580:CDS
GAACGATTCTGATGATGTAT+CGG 0.557299 8_2:-4016224 None:intergenic
TTAGTAGGGCTCAACTTGAT+CGG 0.558121 8_2:+4016614 Msa1228580:CDS
GGAGATGTTGCAGATTAGTA+GGG 0.562230 8_2:+4016600 Msa1228580:CDS
GGAACAATAAGAGGCAGTGC+CGG 0.563483 8_2:-4020100 None:intergenic
AACTGCAAGTGTTAATCTGA+AGG 0.564018 8_2:-4015533 None:intergenic
GCGGTTGGAATGAACCAGTT+CGG 0.568073 8_2:-4016061 None:intergenic
GGAATTTGTTGCCGGCGGTG+AGG 0.568540 8_2:+4016450 Msa1228580:CDS
AAAATTCTCCCAGTTCCAAA+CGG 0.570472 8_2:-4016119 None:intergenic
CAAAAGGAAAATGCATCAGA+TGG 0.571615 8_2:-4019517 None:intergenic
AACACGTCGCCGGAAAACTC+CGG 0.571679 8_2:+4015979 Msa1228580:CDS
TAAACTCGACAACGAAGAAA+CGG 0.572183 8_2:+4015700 Msa1228580:CDS
AACCGGTATTTAACTGCCAA+TGG 0.572575 8_2:-4016921 None:intergenic
CCGTTCATATGCCATAAAGT+AGG 0.573938 8_2:+4020008 Msa1228580:CDS
AGAACGCCGCCGTCTCATGA+AGG 0.575210 8_2:+4015736 Msa1228580:CDS
TTGGACCTCAAAGCCACCAT+TGG 0.576071 8_2:+4016905 Msa1228580:CDS
GGAGACAGCGAGAGGTTCAC+CGG 0.577083 8_2:-4015852 None:intergenic
CAGTGTCTGGAACACGGTCA+AGG 0.579288 8_2:-4019558 None:intergenic
TGCCAAAAGCCGGTCCAAAG+TGG 0.581464 8_2:-4016991 None:intergenic
CAAACTCTTTCAGGAAGTGA+AGG 0.582816 8_2:+4017022 Msa1228580:CDS
TCTGCAGAGTGAAGAATGCA+AGG 0.582873 8_2:+4017886 Msa1228580:CDS
TATCGCCGGTTTGAAGTCAT+CGG 0.584629 8_2:+4016417 Msa1228580:CDS
GATAGAGATGAAGTTATCGC+CGG 0.585630 8_2:+4016403 Msa1228580:CDS
TGTAACTCTCTTCGCCTCAC+CGG 0.588335 8_2:+4015806 Msa1228580:CDS
GCCGGTTCACTTGAAGGACC+GGG 0.588752 8_2:+4016948 Msa1228580:CDS
GCCGGTCCAAAGTGGAGCAG+AGG 0.591183 8_2:-4016983 None:intergenic
CATCTCCGATAACCGATCTC+CGG 0.591527 8_2:-4016584 None:intergenic
GGTTCGGTGTAGTGATTGGG+AGG 0.592402 8_2:+4016354 Msa1228580:CDS
CAGTTTCTGAAACACGTCGC+CGG 0.594202 8_2:+4015969 Msa1228580:CDS
AGTGTCTGGAACACGGTCAA+GGG 0.596369 8_2:-4019557 None:intergenic
GTGCAACCCATTGTTTCAGG+TGG 0.596711 8_2:-4015671 None:intergenic
GTGGTTGTTACGGTGGCTGG+AGG 0.605382 8_2:-4015935 None:intergenic
AAGACCATTGATGGCAGCTG+AGG 0.609697 8_2:-4018002 None:intergenic
GGCGGCGAAGGTGGAGAGTG+CGG 0.611743 8_2:-4015831 None:intergenic
CGGTATTTAACTGCCAATGG+TGG 0.612149 8_2:-4016918 None:intergenic
TCCTTAATTTCCTATAAGAG+GGG 0.612933 8_2:-4020173 None:intergenic
GACGTGTTTCAGAAACTGTA+CGG 0.616647 8_2:-4015964 None:intergenic
ACTGTGTTGATGAAAACGGC+GGG 0.618606 8_2:-4015884 None:intergenic
GAAAACGGCGGGAATGTCGT+CGG 0.622356 8_2:-4015873 None:intergenic
TGGAATAATTCCAAACTCTG+TGG 0.623866 8_2:+4019759 Msa1228580:CDS
AACATAGAATCATTCACATG+TGG 0.629855 8_2:+4019221 Msa1228580:intron
TGAAAAGTTGAAGCATTCGA+AGG 0.634173 8_2:+4019867 Msa1228580:CDS
CTTGAATCGGAGAGAATGTG+TGG 0.636876 8_2:-4016010 None:intergenic
TCGATTGGAAACGGAACGTG+AGG 0.636903 8_2:+4016330 Msa1228580:CDS
TGAAAACTGACACAGACAAG+TGG 0.640095 8_2:+4020240 Msa1228580:three_prime_UTR
CAGAGTGAAGAATGCAAGGG+AGG 0.640119 8_2:+4017890 Msa1228580:CDS
CCTACTTTATGGCATATGAA+CGG 0.642947 8_2:-4020008 None:intergenic
GTTCTGCTTTGGCTTTCGAG+GGG 0.645485 8_2:+4020048 Msa1228580:three_prime_UTR
TGACAAAGCTCAACAAGCTG+AGG 0.646039 8_2:-4018043 None:intergenic
TGGAGAGTGCGGAACCGGTG+AGG 0.647266 8_2:-4015820 None:intergenic
AAACCAAGGCTACCATAACA+AGG 0.647826 8_2:+4017936 Msa1228580:CDS
TCACCGGAATTTGTTGCCGG+CGG 0.648273 8_2:+4016445 Msa1228580:CDS
AAGCGACAGTGTCTGGAACA+CGG 0.656033 8_2:-4019564 None:intergenic
CGCTCGCCACTACCTCGCGG+CGG 0.656448 8_2:+4015769 Msa1228580:CDS
ATTGCTTAACTGATATTACG+TGG 0.657821 8_2:-4019618 None:intergenic
CCTCACCGGAACCTCACCGC+CGG 0.660549 8_2:-4016461 None:intergenic
ATGTCGTCGGAGACAGCGAG+AGG 0.661397 8_2:-4015860 None:intergenic
AAACTGACACAGACAAGTGG+AGG 0.665167 8_2:+4020243 Msa1228580:three_prime_UTR
TCGACAACGAAGAAACGGTA+CGG 0.665354 8_2:+4015705 Msa1228580:CDS
TACTGTGTTGATGAAAACGG+CGG 0.666428 8_2:-4015885 None:intergenic
CTGCAGAGTGAAGAATGCAA+GGG 0.667767 8_2:+4017887 Msa1228580:CDS
AACAAAGATAGACAAAACCA+AGG 0.673276 8_2:+4017922 Msa1228580:CDS
TCCGGTGAGGAAAGACGAGG+AGG 0.676721 8_2:+4016474 Msa1228580:CDS
CCGGCGGTGAGGTTCCGGTG+AGG 0.679781 8_2:+4016461 Msa1228580:CDS
AATCAGAGTGCGCCGCCGCG+AGG 0.683669 8_2:-4015781 None:intergenic
TCTGTGGAAGATATTGGACA+AGG 0.692232 8_2:+4019775 Msa1228580:CDS
AAACTGCTTCCTTCATGAGA+CGG 0.693412 8_2:-4015745 None:intergenic
CTGCTTCCTTCATGAGACGG+CGG 0.729263 8_2:-4015742 None:intergenic
GACAGCGAGAGGTTCACCGG+CGG 0.729279 8_2:-4015849 None:intergenic
ACAACGAAGAAACGGTACGG+CGG 0.748653 8_2:+4015708 Msa1228580:CDS
TTACGCTCGCCACTACCTCG+CGG 0.753107 8_2:+4015766 Msa1228580:CDS
GGTTCCGGTGAGGAAAGACG+AGG 0.762077 8_2:+4016471 Msa1228580:CDS
GGTTCACTTGAAGGACCGGG+CGG 0.787079 8_2:+4016951 Msa1228580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATCATATATTTAAATTG+GGG + chr8_2:4018176-4018195 Msa1228580:intron 10.0%
!! TATATAGTACTAATAATTGA+TGG + chr8_2:4017161-4017180 Msa1228580:intron 15.0%
!! TAATCATATATTTAAATTGG+GGG + chr8_2:4018177-4018196 Msa1228580:intron 15.0%
!!! CAAATACTATTTCAAAATTT+TGG - chr8_2:4018379-4018398 None:intergenic 15.0%
!! AAATAGTATTTGAGAAAATA+TGG + chr8_2:4018387-4018406 Msa1228580:intron 15.0%
!! ATGAAATTGGAAAATTATAA+TGG + chr8_2:4018929-4018948 Msa1228580:intron 15.0%
!! TATTGAAATTTGAATTGTAA+GGG - chr8_2:4019006-4019025 None:intergenic 15.0%
!! ATATTGAAATTTGAATTGTA+AGG - chr8_2:4019007-4019026 None:intergenic 15.0%
!!! TATAAACTTGATAATCATAA+AGG + chr8_2:4019024-4019043 Msa1228580:intron 15.0%
!! ATTATAAATTACCTACTTTA+TGG - chr8_2:4020022-4020041 None:intergenic 15.0%
!! AGATTTATATCTGAAGAAAT+TGG + chr8_2:4016763-4016782 Msa1228580:intron 20.0%
!!! TAGTTTAGGTTGAATTAAAA+AGG - chr8_2:4017715-4017734 None:intergenic 20.0%
!!! ATTTAGAGTTAATTTGTGAA+GGG - chr8_2:4017760-4017779 None:intergenic 20.0%
!!! TATTTAGAGTTAATTTGTGA+AGG - chr8_2:4017761-4017780 None:intergenic 20.0%
!! TCTAAATACTCATCTAAATT+AGG + chr8_2:4017773-4017792 Msa1228580:intron 20.0%
!!! TTTTGCTTAATTGCTTAATT+AGG + chr8_2:4017820-4017839 Msa1228580:intron 20.0%
!!! ATTTTCTAGTAAAATGAGAT+TGG + chr8_2:4018242-4018261 Msa1228580:intron 20.0%
!!! ATACTATTTCAAAATTTTGG+TGG - chr8_2:4018376-4018395 None:intergenic 20.0%
!! ATTTGAGAGTTAATTATCAA+TGG + chr8_2:4018482-4018501 Msa1228580:intron 20.0%
!! TCTCAATACTAGTATATATA+TGG - chr8_2:4019062-4019081 None:intergenic 20.0%
!! AAATAGAATCACAAAATTCT+TGG - chr8_2:4020152-4020171 None:intergenic 20.0%
!!! TTTTACTGTGTTGATGAAAA+CGG - chr8_2:4015891-4015910 None:intergenic 25.0%
!! GGATTTAATGTTTTTCGTTA+TGG + chr8_2:4016784-4016803 Msa1228580:intron 25.0%
!! TACGATAAATTTTCATGTTG+AGG + chr8_2:4017264-4017283 Msa1228580:intron 25.0%
!!! TGATTCAATTTCGAGTTATA+AGG + chr8_2:4017500-4017519 Msa1228580:intron 25.0%
!!! TAAAAAGGAAGTGACTTTTT+TGG - chr8_2:4017700-4017719 None:intergenic 25.0%
!! TTGCAAGTTCATATTAGTTT+AGG - chr8_2:4017729-4017748 None:intergenic 25.0%
! ACTAATTATTGTAACATCTC+AGG + chr8_2:4017967-4017986 Msa1228580:CDS 25.0%
! TGTGTTTGATATTCAATTCA+AGG + chr8_2:4018299-4018318 Msa1228580:intron 25.0%
! CATATATGTACACATTCAAT+TGG + chr8_2:4018331-4018350 Msa1228580:intron 25.0%
! AACTTGATTCTACTGTATTA+TGG - chr8_2:4018529-4018548 None:intergenic 25.0%
! AGTGAACAAAAAATAACTTC+AGG - chr8_2:4018637-4018656 None:intergenic 25.0%
!!! GAAGTTATTTTTTGTTCACT+AGG + chr8_2:4018637-4018656 Msa1228580:intron 25.0%
!!! ATAAAATGGTTTGCTCAAAT+GGG - chr8_2:4018771-4018790 None:intergenic 25.0%
!!! AATAAAATGGTTTGCTCAAA+TGG - chr8_2:4018772-4018791 None:intergenic 25.0%
! GTAACTGTCAACAAATAAAA+TGG - chr8_2:4018785-4018804 None:intergenic 25.0%
!!! CTCTTTGAAATTTTATGATC+CGG - chr8_2:4019405-4019424 None:intergenic 25.0%
!! GTTTTACAACTCATATTCTA+TGG + chr8_2:4019729-4019748 Msa1228580:CDS 25.0%
! ATAATTAATCTGTTCTGCTT+TGG + chr8_2:4020037-4020056 Msa1228580:three_prime_UTR 25.0%
! ACAACAAAATAGTTAGAACT+TGG - chr8_2:4020124-4020143 None:intergenic 25.0%
! TTATAGGAAATTAAGGAGAT+TGG + chr8_2:4020179-4020198 Msa1228580:three_prime_UTR 25.0%
ATATATACCACCTGAAACAA+TGG + chr8_2:4015664-4015683 Msa1228580:exon 30.0%
TATATACCACCTGAAACAAT+GGG + chr8_2:4015665-4015684 Msa1228580:exon 30.0%
!! TATGAAGATGAATTTTCGAG+TGG - chr8_2:4016261-4016280 None:intergenic 30.0%
!! AAGAGAGAATTTTGAGAAAG+CGG + chr8_2:4016555-4016574 Msa1228580:CDS 30.0%
AACAATGTAAAGCTCTTGTA+CGG + chr8_2:4017117-4017136 Msa1228580:intron 30.0%
! TGCACATCATAATTTTGTTG+AGG + chr8_2:4017396-4017415 Msa1228580:intron 30.0%
AACAAAGATAGACAAAACCA+AGG + chr8_2:4017922-4017941 Msa1228580:CDS 30.0%
ATGTCTCTCTATTCTACTAT+TGG + chr8_2:4018071-4018090 Msa1228580:intron 30.0%
CTTATATCTAAGTTCAGAGA+GGG - chr8_2:4018098-4018117 None:intergenic 30.0%
ACTTATATCTAAGTTCAGAG+AGG - chr8_2:4018099-4018118 None:intergenic 30.0%
TATCTTATTCACATATGAGC+TGG + chr8_2:4018132-4018151 Msa1228580:intron 30.0%
GTATTTGCACAATGTATGAA+AGG + chr8_2:4018202-4018221 Msa1228580:intron 30.0%
ATTGGTATTCGTACTTTCAT+TGG + chr8_2:4018349-4018368 Msa1228580:intron 30.0%
TTGGTATTCGTACTTTCATT+GGG + chr8_2:4018350-4018369 Msa1228580:intron 30.0%
! AATACAGTAGAATCAAGTTG+TGG + chr8_2:4018530-4018549 Msa1228580:intron 30.0%
!!! TATTTTTTGTTCACTAGGTG+TGG + chr8_2:4018642-4018661 Msa1228580:intron 30.0%
!! TAAAATGGTTTGCTCAAATG+GGG - chr8_2:4018770-4018789 None:intergenic 30.0%
AGGGAAACAATTATACAGAA+TGG - chr8_2:4018886-4018905 None:intergenic 30.0%
CTGTATAATTGTTTCCCTTA+AGG + chr8_2:4018888-4018907 Msa1228580:intron 30.0%
CAATTTCATATACTATTGCC+AGG - chr8_2:4018918-4018937 None:intergenic 30.0%
CTGGCAATAGTATATGAAAT+TGG + chr8_2:4018916-4018935 Msa1228580:intron 30.0%
GAACATAGTAGCATATTTCT+TGG + chr8_2:4018951-4018970 Msa1228580:intron 30.0%
! ATTTCTTGGTAAAGCATCTT+TGG + chr8_2:4018965-4018984 Msa1228580:intron 30.0%
TTGATGCACAACAATAATGT+AGG - chr8_2:4019133-4019152 None:intergenic 30.0%
CATGTGAATGATTCTATGTT+TGG - chr8_2:4019221-4019240 None:intergenic 30.0%
AACATAGAATCATTCACATG+TGG + chr8_2:4019221-4019240 Msa1228580:intron 30.0%
!!! GACTTTATTTTGTCACTACA+TGG + chr8_2:4019424-4019443 Msa1228580:CDS 30.0%
!! TTATTTTGTCACTACATGGT+TGG + chr8_2:4019428-4019447 Msa1228580:CDS 30.0%
ATTGCTTAACTGATATTACG+TGG - chr8_2:4019621-4019640 None:intergenic 30.0%
GTCAAGAACTCAAGATTAAA+AGG + chr8_2:4019641-4019660 Msa1228580:CDS 30.0%
TACAACTCATATTCTATGGT+TGG + chr8_2:4019733-4019752 Msa1228580:CDS 30.0%
! TCATATTCTATGGTTGGTAT+TGG + chr8_2:4019739-4019758 Msa1228580:CDS 30.0%
ACACTTAATAATCTACAGAC+AGG + chr8_2:4019913-4019932 Msa1228580:CDS 30.0%
TTAGAACTTGGAACAATAAG+AGG - chr8_2:4020112-4020131 None:intergenic 30.0%
! ATTCTATTTTCCCCTCTTAT+AGG + chr8_2:4020163-4020182 Msa1228580:three_prime_UTR 30.0%
TCCTTAATTTCCTATAAGAG+GGG - chr8_2:4020176-4020195 None:intergenic 30.0%
CTCCTTAATTTCCTATAAGA+GGG - chr8_2:4020177-4020196 None:intergenic 30.0%
TCTCCTTAATTTCCTATAAG+AGG - chr8_2:4020178-4020197 None:intergenic 30.0%
TTAATCTGAAGGACTAGGTT+AGG - chr8_2:4015525-4015544 None:intergenic 35.0%
AAGTGTTAATCTGAAGGACT+AGG - chr8_2:4015530-4015549 None:intergenic 35.0%
AACTGCAAGTGTTAATCTGA+AGG - chr8_2:4015536-4015555 None:intergenic 35.0%
TAAACTCGACAACGAAGAAA+CGG + chr8_2:4015700-4015719 Msa1228580:CDS 35.0%
!!! TGTTGATTCATTTTGATGCG+CGG - chr8_2:4016083-4016102 None:intergenic 35.0%
AAAATTCTCCCAGTTCCAAA+CGG - chr8_2:4016122-4016141 None:intergenic 35.0%
CTCCAAATTCCGAATATTTC+CGG + chr8_2:4016158-4016177 Msa1228580:CDS 35.0%
! GGATTTTCCGGTTTATGATT+CGG - chr8_2:4016199-4016218 None:intergenic 35.0%
! GAACGATTCTGATGATGTAT+CGG - chr8_2:4016227-4016246 None:intergenic 35.0%
TCAGAATCACAAGACTGAAA+CGG + chr8_2:4016294-4016313 Msa1228580:CDS 35.0%
AGAACTAGATCGATTGGAAA+CGG + chr8_2:4016321-4016340 Msa1228580:CDS 35.0%
! TTTTTCCGATGACTTCAAAC+CGG - chr8_2:4016425-4016444 None:intergenic 35.0%
AAAGGTGAAGATGAAGCATA+AGG + chr8_2:4016507-4016526 Msa1228580:CDS 35.0%
ATGGGAAAACAAACTCTTTC+AGG + chr8_2:4017013-4017032 Msa1228580:CDS 35.0%
ACAATGTAAAGCTCTTGTAC+GGG + chr8_2:4017118-4017137 Msa1228580:intron 35.0%
! ATCACAGAATCAGTCACTTT+CGG + chr8_2:4017198-4017217 Msa1228580:intron 35.0%
AACACTAAAACACCTAAAGC+AGG - chr8_2:4017309-4017328 None:intergenic 35.0%
CAGTGAGTTAAGCAAAATCA+CGG - chr8_2:4017459-4017478 None:intergenic 35.0%
TTATAAGGAATGAGTCGAGT+AGG + chr8_2:4017515-4017534 Msa1228580:intron 35.0%
TATAAGGAATGAGTCGAGTA+GGG + chr8_2:4017516-4017535 Msa1228580:intron 35.0%
TTGCTTAATTAGGCTAGAGA+AGG + chr8_2:4017830-4017849 Msa1228580:intron 35.0%
TAGTGACTGTAACCTTGTTA+TGG - chr8_2:4017951-4017970 None:intergenic 35.0%
! TTCCTGACTTTGTTGACTAA+TGG - chr8_2:4018269-4018288 None:intergenic 35.0%
CTACTGTATTATGGCAATAC+AGG - chr8_2:4018520-4018539 None:intergenic 35.0%
AACATTGTACAGCAAGTTTG+TGG + chr8_2:4019277-4019296 Msa1228580:CDS 35.0%
!! CTTTTGATGATTGGTTCACA+AGG - chr8_2:4019303-4019322 None:intergenic 35.0%
CTTGTGAACCAATCATCAAA+AGG + chr8_2:4019301-4019320 Msa1228580:CDS 35.0%
!! TTGAGTGACCTTTTGATGAT+TGG - chr8_2:4019312-4019331 None:intergenic 35.0%
!! CTTGGCATTCAAGTTTTATC+CGG + chr8_2:4019383-4019402 Msa1228580:CDS 35.0%
! TTGTTGTTGTCATTGTTGAC+TGG - chr8_2:4019472-4019491 None:intergenic 35.0%
CAAAAGGAAAATGCATCAGA+TGG - chr8_2:4019520-4019539 None:intergenic 35.0%
! GATGCATTTTCCTTTTGTGA+TGG + chr8_2:4019523-4019542 Msa1228580:CDS 35.0%
!! CATTTTCCTTTTGTGATGGA+TGG + chr8_2:4019527-4019546 Msa1228580:CDS 35.0%
TAGTTTCCATCCATCACAAA+AGG - chr8_2:4019536-4019555 None:intergenic 35.0%
TGGAATAATTCCAAACTCTG+TGG + chr8_2:4019759-4019778 Msa1228580:CDS 35.0%
!!! CCAATTTTTTCTCAGCAAGA+GGG - chr8_2:4019816-4019835 None:intergenic 35.0%
!!! TCCAATTTTTTCTCAGCAAG+AGG - chr8_2:4019817-4019836 None:intergenic 35.0%
! TGAAAAGTTGAAGCATTCGA+AGG + chr8_2:4019867-4019886 Msa1228580:CDS 35.0%
CCTACTTTATGGCATATGAA+CGG - chr8_2:4020011-4020030 None:intergenic 35.0%
!!! GTATAAAGGCCGATTTTTTC+CGG + chr8_2:4020081-4020100 Msa1228580:three_prime_UTR 35.0%
TCCCCTCTTATAGGAAATTA+AGG + chr8_2:4020172-4020191 Msa1228580:three_prime_UTR 35.0%
!! AAGGAGATTGGCTCTTTTAT+TGG + chr8_2:4020191-4020210 Msa1228580:three_prime_UTR 35.0%
AAACTGCTTCCTTCATGAGA+CGG - chr8_2:4015748-4015767 None:intergenic 40.0%
! TACTGTGTTGATGAAAACGG+CGG - chr8_2:4015888-4015907 None:intergenic 40.0%
!! ACGGAGTTTTGTGGTTGTTA+CGG - chr8_2:4015948-4015967 None:intergenic 40.0%
! AGAAACTGTACGGAGTTTTG+TGG - chr8_2:4015957-4015976 None:intergenic 40.0%
GACGTGTTTCAGAAACTGTA+CGG - chr8_2:4015967-4015986 None:intergenic 40.0%
CGTTGAACAAACGCTTGAAT+CGG - chr8_2:4016026-4016045 None:intergenic 40.0%
!! CTTCTGAAACTTCTTCCGTT+TGG + chr8_2:4016104-4016123 Msa1228580:CDS 40.0%
! AAACTTCTTCCGTTTGGAAC+TGG + chr8_2:4016110-4016129 Msa1228580:CDS 40.0%
! AACTTCTTCCGTTTGGAACT+GGG + chr8_2:4016111-4016130 Msa1228580:CDS 40.0%
TATTCGGAATTTGGAGGAGA+AGG - chr8_2:4016154-4016173 None:intergenic 40.0%
CGGAAATATTCGGAATTTGG+AGG - chr8_2:4016160-4016179 None:intergenic 40.0%
TGCCGGAAATATTCGGAATT+TGG - chr8_2:4016163-4016182 None:intergenic 40.0%
AGAACGTTGCCGGAAATATT+CGG - chr8_2:4016170-4016189 None:intergenic 40.0%
!! TTTTCCGGTTTATGATTCGG+CGG - chr8_2:4016196-4016215 None:intergenic 40.0%
! GTATCGGATTCTGGATTTTC+CGG - chr8_2:4016211-4016230 None:intergenic 40.0%
! TCTGATGATGTATCGGATTC+TGG - chr8_2:4016220-4016239 None:intergenic 40.0%
GGAACAAGAACTAGATCGAT+TGG + chr8_2:4016315-4016334 Msa1228580:CDS 40.0%
GATAGAGATGAAGTTATCGC+CGG + chr8_2:4016403-4016422 Msa1228580:CDS 40.0%
GAAGTCATCGGAAAAATCAC+CGG + chr8_2:4016429-4016448 Msa1228580:CDS 40.0%
!! AAATCACCGGAATTTGTTGC+CGG + chr8_2:4016442-4016461 Msa1228580:CDS 40.0%
!! AGAGAATTTTGAGAAAGCGG+CGG + chr8_2:4016558-4016577 Msa1228580:CDS 40.0%
GGAGATGTTGCAGATTAGTA+GGG + chr8_2:4016600-4016619 Msa1228580:CDS 40.0%
TTAGTAGGGCTCAACTTGAT+CGG + chr8_2:4016614-4016633 Msa1228580:CDS 40.0%
GTTTGCTTACTGAGAAAGTG+CGG + chr8_2:4016684-4016703 Msa1228580:intron 40.0%
! TTTGCTTACTGAGAAAGTGC+GGG + chr8_2:4016685-4016704 Msa1228580:intron 40.0%
CACCATTGGCAGTTAAATAC+CGG + chr8_2:4016919-4016938 Msa1228580:CDS 40.0%
AACCGGTATTTAACTGCCAA+TGG - chr8_2:4016924-4016943 None:intergenic 40.0%
!! GTTAAATACCGGTTTGATGC+CGG + chr8_2:4016930-4016949 Msa1228580:CDS 40.0%
CAAACTCTTTCAGGAAGTGA+AGG + chr8_2:4017022-4017041 Msa1228580:CDS 40.0%
! CTGATGATGTTTCCTGCTTT+AGG + chr8_2:4017294-4017313 Msa1228580:intron 40.0%
CTTAACTCACTGCGAAACAA+GGG + chr8_2:4017467-4017486 Msa1228580:intron 40.0%
CATGCTAATCAAACCACGTT+TGG - chr8_2:4017585-4017604 None:intergenic 40.0%
CGTGGTTTGATTAGCATGTT+TGG + chr8_2:4017587-4017606 Msa1228580:intron 40.0%
GTGTGAAGATTGAGCATGAA+AGG + chr8_2:4017852-4017871 Msa1228580:CDS 40.0%
AAACCAAGGCTACCATAACA+AGG + chr8_2:4017936-4017955 Msa1228580:CDS 40.0%
! TAACCTTGTTATGGTAGCCT+TGG - chr8_2:4017942-4017961 None:intergenic 40.0%
!! AGCTTGTTGAGCTTTGTCAT+GGG + chr8_2:4018047-4018066 Msa1228580:CDS 40.0%
GACCATTAGTCAACAAAGTC+AGG + chr8_2:4018264-4018283 Msa1228580:intron 40.0%
GTCAACAAAGTCAGGAACTT+AGG + chr8_2:4018272-4018291 Msa1228580:intron 40.0%
! TATTGCCAGGTTGACCTTAA+GGG - chr8_2:4018905-4018924 None:intergenic 40.0%
!! CAATGTTGCTTTGCGTTTCA+TGG - chr8_2:4019264-4019283 None:intergenic 40.0%
AACATCCGAATCACACAAAC+AGG + chr8_2:4019330-4019349 Msa1228580:CDS 40.0%
AGTTGCCTGTTTGTGTGATT+CGG - chr8_2:4019338-4019357 None:intergenic 40.0%
CAGCAGCATTAGCAACAAAA+TGG + chr8_2:4019492-4019511 Msa1228580:CDS 40.0%
GAAAACTGACACTGCTTACA+AGG + chr8_2:4019663-4019682 Msa1228580:CDS 40.0%
CCAATATCTTCCACAGAGTT+TGG - chr8_2:4019772-4019791 None:intergenic 40.0%
CCAAACTCTGTGGAAGATAT+TGG + chr8_2:4019769-4019788 Msa1228580:CDS 40.0%
TCTGTGGAAGATATTGGACA+AGG + chr8_2:4019775-4019794 Msa1228580:CDS 40.0%
CCCTCTTGCTGAGAAAAAAT+TGG + chr8_2:4019813-4019832 Msa1228580:CDS 40.0%
CCGTTCATATGCCATAAAGT+AGG + chr8_2:4020008-4020027 Msa1228580:CDS 40.0%
TGAAAACTGACACAGACAAG+TGG + chr8_2:4020240-4020259 Msa1228580:three_prime_UTR 40.0%
TCGACAACGAAGAAACGGTA+CGG + chr8_2:4015705-4015724 Msa1228580:CDS 45.0%
! ACTGTGTTGATGAAAACGGC+GGG - chr8_2:4015887-4015906 None:intergenic 45.0%
!! GAGTTTTGTGGTTGTTACGG+TGG - chr8_2:4015945-4015964 None:intergenic 45.0%
AGAATGTGTGGAAGCTTCAC+CGG - chr8_2:4016001-4016020 None:intergenic 45.0%
CTTGAATCGGAGAGAATGTG+TGG - chr8_2:4016013-4016032 None:intergenic 45.0%
!! GATTCATTTTGATGCGCGGT+TGG - chr8_2:4016079-4016098 None:intergenic 45.0%
TCAACCGCCGAATCATAAAC+CGG + chr8_2:4016189-4016208 Msa1228580:CDS 45.0%
!! TATCGCCGGTTTGAAGTCAT+CGG + chr8_2:4016417-4016436 Msa1228580:CDS 45.0%
CGAGGAGGTGAATGAAGAAA+AGG + chr8_2:4016489-4016508 Msa1228580:CDS 45.0%
CGGAGATGTTGCAGATTAGT+AGG + chr8_2:4016599-4016618 Msa1228580:CDS 45.0%
TGAGCAAAGTGAGCTCAACT+TGG + chr8_2:4016886-4016905 Msa1228580:CDS 45.0%
CGGTATTTAACTGCCAATGG+TGG - chr8_2:4016921-4016940 None:intergenic 45.0%
!! TTTGATGCCGGTTCACTTGA+AGG + chr8_2:4016942-4016961 Msa1228580:CDS 45.0%
! TGTTTTCCCATGCCAAAAGC+CGG - chr8_2:4017004-4017023 None:intergenic 45.0%
TCTTGTACGGGTAGTGTAGT+GGG + chr8_2:4017130-4017149 Msa1228580:intron 45.0%
GCAGGAAACATCATCAGCAA+AGG - chr8_2:4017291-4017310 None:intergenic 45.0%
GCTTAACTCACTGCGAAACA+AGG + chr8_2:4017466-4017485 Msa1228580:intron 45.0%
AGGGTGAGTGGATGGATATT+AGG + chr8_2:4017535-4017554 Msa1228580:intron 45.0%
ACGTTCAAATGCACCAAACG+TGG + chr8_2:4017569-4017588 Msa1228580:intron 45.0%
TCTGCAGAGTGAAGAATGCA+AGG + chr8_2:4017886-4017905 Msa1228580:CDS 45.0%
CTGCAGAGTGAAGAATGCAA+GGG + chr8_2:4017887-4017906 Msa1228580:CDS 45.0%
! AGCTGCCATCAATGGTCTTA+GGG + chr8_2:4018006-4018025 Msa1228580:CDS 45.0%
AGAGTCCCTAAGACCATTGA+TGG - chr8_2:4018014-4018033 None:intergenic 45.0%
TGACAAAGCTCAACAAGCTG+AGG - chr8_2:4018046-4018065 None:intergenic 45.0%
!! CAGCTTGTTGAGCTTTGTCA+TGG + chr8_2:4018046-4018065 Msa1228580:CDS 45.0%
GAGCAACACTGAGTCTGAAT+TGG - chr8_2:4018422-4018441 None:intergenic 45.0%
CACTAGGTGTGGTGTATGAA+TGG + chr8_2:4018653-4018672 Msa1228580:intron 45.0%
CAGCGAGTTCTGCGAAATTA+CGG + chr8_2:4018681-4018700 Msa1228580:intron 45.0%
TGTTTCCCTTAAGGTCAACC+TGG + chr8_2:4018897-4018916 Msa1228580:intron 45.0%
! CTATTGCCAGGTTGACCTTA+AGG - chr8_2:4018906-4018925 None:intergenic 45.0%
TGCTTTGCGTTTCATGGTAC+TGG - chr8_2:4019258-4019277 None:intergenic 45.0%
TAGAATCAGCTCTGTCAGCT+TGG + chr8_2:4019365-4019384 Msa1228580:CDS 45.0%
GGAAGATATTGGACAAGGCT+TGG + chr8_2:4019780-4019799 Msa1228580:CDS 45.0%
! GAAGCATTCGAAGGCTATTG+AGG + chr8_2:4019876-4019895 Msa1228580:CDS 45.0%
AATCTACAGACAGGTTTGCC+TGG + chr8_2:4019922-4019941 Msa1228580:CDS 45.0%
AGGTTTGCCTGGAATGTTTC+AGG + chr8_2:4019933-4019952 Msa1228580:CDS 45.0%
GTCAATGCCTGAAACATTCC+AGG - chr8_2:4019943-4019962 None:intergenic 45.0%
!! TGTTCTGCTTTGGCTTTCGA+GGG + chr8_2:4020047-4020066 Msa1228580:three_prime_UTR 45.0%
GGGGTGTGAGTTCAGTATAA+AGG + chr8_2:4020067-4020086 Msa1228580:three_prime_UTR 45.0%
AAACTGACACAGACAAGTGG+AGG + chr8_2:4020243-4020262 Msa1228580:three_prime_UTR 45.0%
!!! TTTTAATCATATATTTAAAT+TGG + chr8_2:4018174-4018193 Msa1228580:intron 5.0%
!! TTTAATCATATATTTAAATT+GGG + chr8_2:4018175-4018194 Msa1228580:intron 5.0%
GTGCAACCCATTGTTTCAGG+TGG - chr8_2:4015674-4015693 None:intergenic 50.0%
GCTGTGCAACCCATTGTTTC+AGG - chr8_2:4015677-4015696 None:intergenic 50.0%
ACAACGAAGAAACGGTACGG+CGG + chr8_2:4015708-4015727 Msa1228580:CDS 50.0%
TGTAACTCTCTTCGCCTCAC+CGG + chr8_2:4015806-4015825 Msa1228580:CDS 50.0%
TTTGTGGTTGTTACGGTGGC+TGG - chr8_2:4015941-4015960 None:intergenic 50.0%
!! CAGTTTCTGAAACACGTCGC+CGG + chr8_2:4015969-4015988 Msa1228580:CDS 50.0%
! GAAGCTTCACCGGAGTTTTC+CGG - chr8_2:4015991-4016010 None:intergenic 50.0%
CAGTTCGGAAACTCGCTTCT+CGG - chr8_2:4016049-4016068 None:intergenic 50.0%
GCGGTTGGAATGAACCAGTT+CGG - chr8_2:4016064-4016083 None:intergenic 50.0%
GAATTTGGAGGAGAAGGAGG+AGG - chr8_2:4016148-4016167 None:intergenic 50.0%
TCGGAATTTGGAGGAGAAGG+AGG - chr8_2:4016151-4016170 None:intergenic 50.0%
TCGATTGGAAACGGAACGTG+AGG + chr8_2:4016330-4016349 Msa1228580:CDS 50.0%
ATTGGAAACGGAACGTGAGG+AGG + chr8_2:4016333-4016352 Msa1228580:CDS 50.0%
AAACGGAACGTGAGGAGGTT+CGG + chr8_2:4016338-4016357 Msa1228580:CDS 50.0%
!! AGGAGGTTCGGTGTAGTGAT+TGG + chr8_2:4016350-4016369 Msa1228580:CDS 50.0%
!! GGAGGTTCGGTGTAGTGATT+GGG + chr8_2:4016351-4016370 Msa1228580:CDS 50.0%
!! GAATTTTGAGAAAGCGGCGG+TGG + chr8_2:4016561-4016580 Msa1228580:CDS 50.0%
CATCTCCGATAACCGATCTC+CGG - chr8_2:4016587-4016606 None:intergenic 50.0%
TTGGACCTCAAAGCCACCAT+TGG + chr8_2:4016905-4016924 Msa1228580:CDS 50.0%
! AACTGCCAATGGTGGCTTTG+AGG - chr8_2:4016913-4016932 None:intergenic 50.0%
CAAGTGAACCGGCATCAAAC+CGG - chr8_2:4016941-4016960 None:intergenic 50.0%
AAAGTGGAGCAGAGGCTCTT+TGG - chr8_2:4016978-4016997 None:intergenic 50.0%
AAAGAGCCTCTGCTCCACTT+TGG + chr8_2:4016977-4016996 Msa1228580:CDS 50.0%
!! TTTGGACCGGCTTTTGGCAT+GGG + chr8_2:4016995-4017014 Msa1228580:CDS 50.0%
CTCTTGTACGGGTAGTGTAG+TGG + chr8_2:4017129-4017148 Msa1228580:intron 50.0%
AATGAGTCGAGTAGGGTGAG+TGG + chr8_2:4017523-4017542 Msa1228580:intron 50.0%
CAGAGTGAAGAATGCAAGGG+AGG + chr8_2:4017890-4017909 Msa1228580:CDS 50.0%
ACTACCTCAGCTGCCATCAA+TGG + chr8_2:4017998-4018017 Msa1228580:CDS 50.0%
! AAGACCATTGATGGCAGCTG+AGG - chr8_2:4018005-4018024 None:intergenic 50.0%
CAGCTGCCATCAATGGTCTT+AGG + chr8_2:4018005-4018024 Msa1228580:CDS 50.0%
ATTAACCGTCGTTGCTGCAC+AGG - chr8_2:4019086-4019105 None:intergenic 50.0%
TGTGCAGCAACGACGGTTAA+TGG + chr8_2:4019085-4019104 Msa1228580:intron 50.0%
! AGTGTCTGGAACACGGTCAA+GGG - chr8_2:4019560-4019579 None:intergenic 50.0%
! AAGCGACAGTGTCTGGAACA+CGG - chr8_2:4019567-4019586 None:intergenic 50.0%
! TGTTCCAGACACTGTCGCTT+CGG + chr8_2:4019567-4019586 Msa1228580:CDS 50.0%
!! CTGTTCTGCTTTGGCTTTCG+AGG + chr8_2:4020046-4020065 Msa1228580:three_prime_UTR 50.0%
!! GTTCTGCTTTGGCTTTCGAG+GGG + chr8_2:4020048-4020067 Msa1228580:three_prime_UTR 50.0%
! GAGGCAGTGCCGGAAAAAAT+CGG - chr8_2:4020093-4020112 None:intergenic 50.0%
GGAACAATAAGAGGCAGTGC+CGG - chr8_2:4020103-4020122 None:intergenic 50.0%
CTGCTTCCTTCATGAGACGG+CGG - chr8_2:4015745-4015764 None:intergenic 55.0%
GAAAACGGCGGGAATGTCGT+CGG - chr8_2:4015876-4015895 None:intergenic 55.0%
AACACGTCGCCGGAAAACTC+CGG + chr8_2:4015979-4015998 Msa1228580:CDS 55.0%
CGAGAAGCGAGTTTCCGAAC+TGG + chr8_2:4016047-4016066 Msa1228580:CDS 55.0%
!! GGTTCGGTGTAGTGATTGGG+AGG + chr8_2:4016354-4016373 Msa1228580:CDS 55.0%
!! TCACCGGAATTTGTTGCCGG+CGG + chr8_2:4016445-4016464 Msa1228580:CDS 55.0%
TCACCGCCGGCAACAAATTC+CGG - chr8_2:4016451-4016470 None:intergenic 55.0%
ACCTCCTCGTCTTTCCTCAC+CGG - chr8_2:4016478-4016497 None:intergenic 55.0%
!! TGCCGGTTCACTTGAAGGAC+CGG + chr8_2:4016947-4016966 Msa1228580:CDS 55.0%
!! CTCCACTTTGGACCGGCTTT+TGG + chr8_2:4016989-4017008 Msa1228580:CDS 55.0%
TGCCAAAAGCCGGTCCAAAG+TGG - chr8_2:4016994-4017013 None:intergenic 55.0%
!! CTTTGGACCGGCTTTTGGCA+TGG + chr8_2:4016994-4017013 Msa1228580:CDS 55.0%
! TGTACGGGTAGTGTAGTGGG+AGG + chr8_2:4017133-4017152 Msa1228580:intron 55.0%
! GTACGGGTAGTGTAGTGGGA+GGG + chr8_2:4017134-4017153 Msa1228580:intron 55.0%
AGTCGAGTAGGGTGAGTGGA+TGG + chr8_2:4017527-4017546 Msa1228580:intron 55.0%
GAGAACCTGTGCAGCAACGA+CGG + chr8_2:4019078-4019097 Msa1228580:intron 55.0%
! CAGTGTCTGGAACACGGTCA+AGG - chr8_2:4019561-4019580 None:intergenic 55.0%
ACACAGACAAGTGGAGGAGC+CGG + chr8_2:4020249-4020268 Msa1228580:three_prime_UTR 55.0%
AGAACGCCGCCGTCTCATGA+AGG + chr8_2:4015736-4015755 Msa1228580:CDS 60.0%
TTACGCTCGCCACTACCTCG+CGG + chr8_2:4015766-4015785 Msa1228580:CDS 60.0%
! GAAGGTGGAGAGTGCGGAAC+CGG - chr8_2:4015828-4015847 None:intergenic 60.0%
GGAGACAGCGAGAGGTTCAC+CGG - chr8_2:4015855-4015874 None:intergenic 60.0%
ATGTCGTCGGAGACAGCGAG+AGG - chr8_2:4015863-4015882 None:intergenic 60.0%
GTTGTTACGGTGGCTGGAGG+AGG - chr8_2:4015935-4015954 None:intergenic 60.0%
GTGGTTGTTACGGTGGCTGG+AGG - chr8_2:4015938-4015957 None:intergenic 60.0%
TCGGCGGTTGAGAACGTTGC+CGG - chr8_2:4016180-4016199 None:intergenic 60.0%
!! GGAATTTGTTGCCGGCGGTG+AGG + chr8_2:4016450-4016469 Msa1228580:CDS 60.0%
GGTTCCGGTGAGGAAAGACG+AGG + chr8_2:4016471-4016490 Msa1228580:CDS 60.0%
TCCGGTGAGGAAAGACGAGG+AGG + chr8_2:4016474-4016493 Msa1228580:CDS 60.0%
TTTGAGAAAGCGGCGGTGGC+CGG + chr8_2:4016565-4016584 Msa1228580:CDS 60.0%
GGTGGCCGGAGATCGGTTAT+CGG + chr8_2:4016579-4016598 Msa1228580:CDS 60.0%
! GCCGGTTCACTTGAAGGACC+GGG + chr8_2:4016948-4016967 Msa1228580:CDS 60.0%
GCCCGGTCCTTCAAGTGAAC+CGG - chr8_2:4016952-4016971 None:intergenic 60.0%
GGTTCACTTGAAGGACCGGG+CGG + chr8_2:4016951-4016970 Msa1228580:CDS 60.0%
GCCTCTGCTCCACTTTGGAC+CGG + chr8_2:4016982-4017001 Msa1228580:CDS 60.0%
GCTTCCGAAGCGACAGTGTC+TGG - chr8_2:4019574-4019593 None:intergenic 60.0%
! TGGAGAGTGCGGAACCGGTG+AGG - chr8_2:4015823-4015842 None:intergenic 65.0%
GACAGCGAGAGGTTCACCGG+CGG - chr8_2:4015852-4015871 None:intergenic 65.0%
GTTACGGTGGCTGGAGGAGG+AGG - chr8_2:4015932-4015951 None:intergenic 65.0%
TGTTGCCGGCGGTGAGGTTC+CGG + chr8_2:4016456-4016475 Msa1228580:CDS 65.0%
CAGAGGCTCTTTGGACCGCC+CGG - chr8_2:4016969-4016988 None:intergenic 65.0%
GCCGGTCCAAAGTGGAGCAG+AGG - chr8_2:4016986-4017005 None:intergenic 65.0%
AATCAGAGTGCGCCGCCGCG+AGG - chr8_2:4015784-4015803 None:intergenic 70.0%
GGCGGCGAAGGTGGAGAGTG+CGG - chr8_2:4015834-4015853 None:intergenic 70.0%
GCACTCTCCACCTTCGCCGC+CGG + chr8_2:4015833-4015852 Msa1228580:CDS 70.0%
AGGTTCACCGGCGGCGAAGG+TGG - chr8_2:4015843-4015862 None:intergenic 70.0%
GAGAGGTTCACCGGCGGCGA+AGG - chr8_2:4015846-4015865 None:intergenic 70.0%
GTGGCTGGAGGAGGAGGTGG+CGG - chr8_2:4015926-4015945 None:intergenic 70.0%
ACGGTGGCTGGAGGAGGAGG+TGG - chr8_2:4015929-4015948 None:intergenic 70.0%
CCTCACCGGAACCTCACCGC+CGG - chr8_2:4016464-4016483 None:intergenic 70.0%
AAGCGGCGGTGGCCGGAGAT+CGG + chr8_2:4016572-4016591 Msa1228580:CDS 70.0%
CGCTCGCCACTACCTCGCGG+CGG + chr8_2:4015769-4015788 Msa1228580:CDS 75.0%
AGTGCGCCGCCGCGAGGTAG+TGG - chr8_2:4015778-4015797 None:intergenic 75.0%
CCGGCGGTGAGGTTCCGGTG+AGG + chr8_2:4016461-4016480 Msa1228580:CDS 75.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 4015507 4020272 4015507 ID=Msa1228580;Name=Msa1228580
chr8_2 mRNA 4015507 4020272 4015507 ID=Msa1228580-mRNA-1;Parent=Msa1228580;Name=Msa1228580-mRNA-1;_AED=0.03;_eAED=0.03;_QI=176|1|1|1|1|1|4|243|728
chr8_2 exon 4015507 4016655 4015507 ID=Msa1228580-mRNA-1:exon:1308;Parent=Msa1228580-mRNA-1
chr8_2 exon 4016859 4017043 4016859 ID=Msa1228580-mRNA-1:exon:1309;Parent=Msa1228580-mRNA-1
chr8_2 exon 4017842 4018068 4017842 ID=Msa1228580-mRNA-1:exon:1310;Parent=Msa1228580-mRNA-1
chr8_2 exon 4019228 4020272 4019228 ID=Msa1228580-mRNA-1:exon:1311;Parent=Msa1228580-mRNA-1
chr8_2 five_prime_UTR 4015507 4015682 4015507 ID=Msa1228580-mRNA-1:five_prime_utr;Parent=Msa1228580-mRNA-1
chr8_2 CDS 4015683 4016655 4015683 ID=Msa1228580-mRNA-1:cds;Parent=Msa1228580-mRNA-1
chr8_2 CDS 4016859 4017043 4016859 ID=Msa1228580-mRNA-1:cds;Parent=Msa1228580-mRNA-1
chr8_2 CDS 4017842 4018068 4017842 ID=Msa1228580-mRNA-1:cds;Parent=Msa1228580-mRNA-1
chr8_2 CDS 4019228 4020029 4019228 ID=Msa1228580-mRNA-1:cds;Parent=Msa1228580-mRNA-1
chr8_2 three_prime_UTR 4020030 4020272 4020030 ID=Msa1228580-mRNA-1:three_prime_utr;Parent=Msa1228580-mRNA-1
Gene Sequence

>Msa1228580

ATGGGTTGCACAGCGTCTAAACTCGACAACGAAGAAACGGTACGGCGGTGCAAAGAACGCCGCCGTCTCATGAAGGAAGCAGTTTACGCTCGCCACTACCTCGCGGCGGCGCACTCTGATTACTGTAACTCTCTTCGCCTCACCGGTTCCGCACTCTCCACCTTCGCCGCCGGTGAACCTCTCGCTGTCTCCGACGACATTCCCGCCGTTTTCATCAACACAGTAAAAACTCGTCAGACACCGCCACCTCCTCCTCCAGCCACCGTAACAACCACAAAACTCCGTACAGTTTCTGAAACACGTCGCCGGAAAACTCCGGTGAAGCTTCCACACATTCTCTCCGATTCAAGCGTTTGTTCAACGCCGAGAAGCGAGTTTCCGAACTGGTTCATTCCAACCGCGCATCAAAATGAATCAACAGCTTCTGAAACTTCTTCCGTTTGGAACTGGGAGAATTTTCATCCTCCTCCTTCTCCTCCAAATTCCGAATATTTCCGGCAACGTTCTCAACCGCCGAATCATAAACCGGAAAATCCAGAATCCGATACATCATCAGAATCGTTCTACAACATGTTCCACTCGAAAATTCATCTTCATAATCAAAATCAGAATCAGAATCACAAGACTGAAACGGAACAAGAACTAGATCGATTGGAAACGGAACGTGAGGAGGTTCGGTGTAGTGATTGGGAGGATTGCTACAGTTCAACAAGCTCATCAGATAGAGATGAAGTTATCGCCGGTTTGAAGTCATCGGAAAAATCACCGGAATTTGTTGCCGGCGGTGAGGTTCCGGTGAGGAAAGACGAGGAGGTGAATGAAGAAAAGGTGAAGATGAAGCATAAGGATTTGAAAGAGATAGTTGAAGTGATAAGAGAGAATTTTGAGAAAGCGGCGGTGGCCGGAGATCGGTTATCGGAGATGTTGCAGATTAGTAGGGCTCAACTTGATCGGAGTTTCAGTCAATTGAAAAAAATAGTGTATCACTCTAGTAATATACTGAGCAAAGTGAGCTCAACTTGGACCTCAAAGCCACCATTGGCAGTTAAATACCGGTTTGATGCCGGTTCACTTGAAGGACCGGGCGGTCCAAAGAGCCTCTGCTCCACTTTGGACCGGCTTTTGGCATGGGAAAACAAACTCTTTCAGGAAGTGAAGGCTAGAGAAGGTGTGAAGATTGAGCATGAAAGGAAATTGTCAACTCTGCAGAGTGAAGAATGCAAGGGAGGTGATGACGAAACAAAGATAGACAAAACCAAGGCTACCATAACAAGGTTACAGTCACTAATTATTGTAACATCTCAGGCTGTGTCTACTACCTCAGCTGCCATCAATGGTCTTAGGGACTCTGATCTTGTTCCTCAGCTTGTTGAGCTTTGTCATGGAATCATTCACATGTGGAGATCAATGCACCAGTACCATGAAACGCAAAGCAACATTGTACAGCAAGTTTGTGGCCTTGTGAACCAATCATCAAAAGGTCACTCAACATCCGAATCACACAAACAGGCAACTCGAGATCTAGAATCAGCTCTGTCAGCTTGGCATTCAAGTTTTATCCGGATCATAAAATTTCAAAGAGACTTTATTTTGTCACTACATGGTTGGTTTAAGCTGAATCTTATTCCAGTCAACAATGACAACAACAACAGCAGCATTAGCAACAAAATGGAACCATCTGATGCATTTTCCTTTTGTGATGGATGGAAACTAGCCCTTGACCGTGTTCCAGACACTGTCGCTTCGGAAGCAATCAAAAGCTTCATCAATGTTGTCCACGTAATATCAGTTAAGCAATGTCAAGAACTCAAGATTAAAAGGAAAACTGACACTGCTTACAAGGAACTTGAGAAAAAATCTTCATCTCTAAAAAACATAGAAAAGAAGTTTTACAACTCATATTCTATGGTTGGTATTGGAATAATTCCAAACTCTGTGGAAGATATTGGACAAGGCTTGGATGCAAGAGACCCTCTTGCTGAGAAAAAATTGGAACTTGAAGCTTGTCAAAGACATGTCGACGATGAAAAGTTGAAGCATTCGAAGGCTATTGAGGTTACAAGAGCTATGACACTTAATAATCTACAGACAGGTTTGCCTGGAATGTTTCAGGCATTGACAAGTTTTTCTTCATTGTTCATTGAAGCTCTTGAATCAGTATGTGACCGTTCATATGCCATAAAGTAG
Protein sequence

>Msa1228580

MGCTASKLDNEETVRRCKERRRLMKEAVYARHYLAAAHSDYCNSLRLTGSALSTFAAGEPLAVSDDIPAVFINTVKTRQTPPPPPPATVTTTKLRTVSETRRRKTPVKLPHILSDSSVCSTPRSEFPNWFIPTAHQNESTASETSSVWNWENFHPPPSPPNSEYFRQRSQPPNHKPENPESDTSSESFYNMFHSKIHLHNQNQNQNHKTETEQELDRLETEREEVRCSDWEDCYSSTSSSDRDEVIAGLKSSEKSPEFVAGGEVPVRKDEEVNEEKVKMKHKDLKEIVEVIRENFEKAAVAGDRLSEMLQISRAQLDRSFSQLKKIVYHSSNILSKVSSTWTSKPPLAVKYRFDAGSLEGPGGPKSLCSTLDRLLAWENKLFQEVKAREGVKIEHERKLSTLQSEECKGGDDETKIDKTKATITRLQSLIIVTSQAVSTTSAAINGLRDSDLVPQLVELCHGIIHMWRSMHQYHETQSNIVQQVCGLVNQSSKGHSTSESHKQATRDLESALSAWHSSFIRIIKFQRDFILSLHGWFKLNLIPVNNDNNNSSISNKMEPSDAFSFCDGWKLALDRVPDTVASEAIKSFINVVHVISVKQCQELKIKRKTDTAYKELEKKSSSLKNIEKKFYNSYSMVGIGIIPNSVEDIGQGLDARDPLAEKKLELEACQRHVDDEKLKHSKAIEVTRAMTLNNLQTGLPGMFQALTSFSSLFIEALESVCDRSYAIK*