AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48648


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48648.t1 MTR_4g032805 96.371 248 8 1 1 248 23 269 0.0 498
MS.gene48648.t1 MTR_2g097690 50.000 62 29 1 109 170 77 136 3.52e-12 63.9
MS.gene48648.t1 MTR_5g022180 36.264 91 55 2 87 176 53 141 7.03e-12 62.4
MS.gene48648.t1 MTR_2g097690 50.000 64 30 1 107 170 75 136 1.34e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48648.t1 AT4G09350 59.389 229 82 3 20 247 28 246 8.25e-96 281
MS.gene48648.t1 AT5G59610 49.231 65 31 1 105 169 71 133 2.68e-11 62.4
MS.gene48648.t1 AT5G59610 49.231 65 31 1 105 169 71 133 6.21e-11 61.6
MS.gene48648.t1 AT5G59610 49.231 65 31 1 105 169 71 133 6.21e-11 61.6
MS.gene48648.t1 AT5G59610 49.231 65 31 1 105 169 71 133 6.88e-11 61.2
MS.gene48648.t1 AT5G59610 49.231 65 31 1 105 169 71 133 6.88e-11 61.2

Find 39 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCACCCAAAAGCTTCTT+AGG 0.196506 4.2:-72188591 None:intergenic
CTACTATTCAATCCAATCTT+AGG 0.223430 4.2:+72188357 MS.gene48648:CDS
GAATATTTGTGCAGATTCTT+AGG 0.256272 4.2:+72189318 MS.gene48648:intron
TCTTATTCTTCTTATTGTTT+TGG 0.269689 4.2:-72188385 None:intergenic
GGAGAAACCTAAGAAGCTTT+TGG 0.298843 4.2:+72188584 MS.gene48648:CDS
TGCATTTGTTTGTTGCTGTT+TGG 0.332246 4.2:-72188559 None:intergenic
ATTGCTTCTACATCAGCTTC+TGG 0.336747 4.2:-72189348 None:intergenic
GCAAGTTCATGCTTCTACTA+AGG 0.365942 4.2:+72188449 MS.gene48648:CDS
GAAACTGAAAATAAAATTAG+AGG 0.366458 4.2:+72189551 MS.gene48648:CDS
AGAGGGAGTGAAGTTGTGTC+TGG 0.393350 4.2:-72189408 None:intergenic
AAGCAATCAAAGTAGCATTC+AGG 0.395851 4.2:+72189364 MS.gene48648:CDS
AAAGTAGAAGATTCTATGAT+TGG 0.404469 4.2:+72189493 MS.gene48648:CDS
TTCTTCAAGGCTACCTCATA+AGG 0.409509 4.2:-72189576 None:intergenic
CAAACTGTAGTGAGAGTTTG+AGG 0.431351 4.2:-72188427 None:intergenic
GGATTGAATAGTAGAATTTG+TGG 0.442017 4.2:-72188348 None:intergenic
AACAGGTTCATAATTCTTCA+AGG 0.442631 4.2:-72189589 None:intergenic
CCAAGACGATCAACCATGTC+TGG 0.451377 4.2:-72189612 None:intergenic
CGATCAACCATGTCTGGAAC+AGG 0.460674 4.2:-72189606 None:intergenic
GATGATGAAGGTTGGATTGG+AGG 0.480496 4.2:+72188531 MS.gene48648:CDS
AATAGTAGAATTTGTGGAGA+AGG 0.498829 4.2:-72188342 None:intergenic
AAAATTAGAGGATCCTTATG+AGG 0.498846 4.2:+72189563 MS.gene48648:CDS
TACATGAGTGATACAGCATA+TGG 0.500284 4.2:-72189706 None:intergenic
AATGATGATGAAGGTTGGAT+TGG 0.510553 4.2:+72188528 MS.gene48648:CDS
TGGATTCTTGTGTCAACTCC+AGG 0.515932 4.2:-72188498 None:intergenic
GGGAGAATGATGATGAAGGT+TGG 0.524754 4.2:+72188523 MS.gene48648:CDS
CTTCATCATCATTCTCCCAA+TGG 0.525735 4.2:-72188518 None:intergenic
GACTTGCAACTTCTTGAGCA+AGG 0.527830 4.2:-72189518 None:intergenic
GACATGGTTGATCGTCTTGG+TGG 0.530101 4.2:+72189615 MS.gene48648:CDS
CCAGACATGGTTGATCGTCT+TGG 0.532935 4.2:+72189612 MS.gene48648:CDS
AGTTGACACAAGAATCCATT+GGG 0.545872 4.2:+72188503 MS.gene48648:CDS
TTATGAACCTGTTCCAGACA+TGG 0.570153 4.2:+72189599 MS.gene48648:CDS
ACTTGCAACTTCTTGAGCAA+GGG 0.574767 4.2:-72189517 None:intergenic
AGCATTCAGGAAACTTTCAA+AGG 0.585269 4.2:+72189377 MS.gene48648:CDS
ACATGGTTGATCGTCTTGGT+GGG 0.606623 4.2:+72189616 MS.gene48648:CDS
CATTGGGAGAATGATGATGA+AGG 0.623650 4.2:+72188519 MS.gene48648:CDS
GAGTTGACACAAGAATCCAT+TGG 0.644255 4.2:+72188502 MS.gene48648:CDS
ACAGCAACAAACAAATGCAG+AGG 0.656916 4.2:+72188563 MS.gene48648:CDS
GCTTCTACTAAGGACACTCA+AGG 0.674014 4.2:+72188459 MS.gene48648:CDS
ATTCTTGTGTCAACTCCAGG+TGG 0.739348 4.2:-72188495 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAGAAGAATAAGATTTTTT+AGG + chr4.2:72188394-72188413 MS.gene48648:CDS 15.0%
!!! TAGTTTAAAATTGAATTTCA+AGG - chr4.2:72188748-72188767 None:intergenic 15.0%
!! GAAACTGAAAATAAAATTAG+AGG + chr4.2:72189551-72189570 MS.gene48648:CDS 20.0%
!!! ATTAAATTCTGTTTTTCTTC+AGG + chr4.2:72189166-72189185 MS.gene48648:intron 20.0%
!!! CGTTTTAAAAAAAGAAAAAC+TGG + chr4.2:72189209-72189228 MS.gene48648:intron 20.0%
!!! TAATTTCATGAATTTTTCTG+AGG - chr4.2:72189442-72189461 None:intergenic 20.0%
!!! TCTTATTCTTCTTATTGTTT+TGG - chr4.2:72188388-72188407 None:intergenic 20.0%
!!! TTTATACTTTTTGTAGGAAA+CGG - chr4.2:72188672-72188691 None:intergenic 20.0%
! AAAGTAGAAGATTCTATGAT+TGG + chr4.2:72189493-72189512 MS.gene48648:CDS 25.0%
! AGACTAAAACAACTATTAGT+CGG + chr4.2:72189001-72189020 MS.gene48648:intron 25.0%
!! TTATTTTCAGTTTCTCTTGT+TGG - chr4.2:72189545-72189564 None:intergenic 25.0%
!!! TGGTGTTTTATACTTTTTGT+AGG - chr4.2:72188678-72188697 None:intergenic 25.0%
AAAATTAGAGGATCCTTATG+AGG + chr4.2:72189563-72189582 MS.gene48648:CDS 30.0%
AACAGGTTCATAATTCTTCA+AGG - chr4.2:72189592-72189611 None:intergenic 30.0%
AATAGTAGAATTTGTGGAGA+AGG - chr4.2:72188345-72188364 None:intergenic 30.0%
AGTTCGTATAAGTATGTGTT+TGG + chr4.2:72188769-72188788 MS.gene48648:intron 30.0%
ATGTCAGAGTTTGATCAAAA+AGG - chr4.2:72189036-72189055 None:intergenic 30.0%
CTACTATTCAATCCAATCTT+AGG + chr4.2:72188357-72188376 MS.gene48648:CDS 30.0%
GAATATTTGTGCAGATTCTT+AGG + chr4.2:72189318-72189337 MS.gene48648:intron 30.0%
GGATTGAATAGTAGAATTTG+TGG - chr4.2:72188351-72188370 None:intergenic 30.0%
GGCAAAATTCAGTTTACAAT+CGG - chr4.2:72188727-72188746 None:intergenic 30.0%
TCAGCTAATGTTGAATAGTT+TGG + chr4.2:72188949-72188968 MS.gene48648:intron 30.0%
TGACAAGAGTTACTGAATAA+TGG + chr4.2:72189296-72189315 MS.gene48648:intron 30.0%
TGATCAAACTCTGACATAAT+CGG + chr4.2:72189039-72189058 MS.gene48648:intron 30.0%
TTTCTCATGATACAAATCTC+TGG - chr4.2:72189261-72189280 None:intergenic 30.0%
! AACCACTTCGATTTTCTATT+AGG + chr4.2:72188700-72188719 MS.gene48648:intron 30.0%
! ACATAATCGGTACATGTTTT+GGG + chr4.2:72189052-72189071 MS.gene48648:intron 30.0%
! AGTTATACTTAGTGCTCTTT+TGG - chr4.2:72188921-72188940 None:intergenic 30.0%
! CGGTGGTTTTTATAAAATCA+CGG - chr4.2:72188892-72188911 None:intergenic 30.0%
! CGTGATTTTATAAAAACCAC+CGG + chr4.2:72188890-72188909 MS.gene48648:intron 30.0%
! TAGAAAATCGAAGTGGTTTT+TGG - chr4.2:72188698-72188717 None:intergenic 30.0%
AAGCAATCAAAGTAGCATTC+AGG + chr4.2:72189364-72189383 MS.gene48648:CDS 35.0%
AGCATTCAGGAAACTTTCAA+AGG + chr4.2:72189377-72189396 MS.gene48648:CDS 35.0%
AGTTGACACAAGAATCCATT+GGG + chr4.2:72188503-72188522 MS.gene48648:CDS 35.0%
ATCTCTGGTAACTAGTAAGT+TGG - chr4.2:72189246-72189265 None:intergenic 35.0%
GACCTAATAGAAAATCGAAG+TGG - chr4.2:72188705-72188724 None:intergenic 35.0%
TACATGAGTGATACAGCATA+TGG - chr4.2:72189709-72189728 None:intergenic 35.0%
TACGACTTGAGTTCAATTGT+TGG + chr4.2:72188973-72188992 MS.gene48648:intron 35.0%
! AATGATGATGAAGGTTGGAT+TGG + chr4.2:72188528-72188547 MS.gene48648:CDS 35.0%
! GACATAATCGGTACATGTTT+TGG + chr4.2:72189051-72189070 MS.gene48648:intron 35.0%
! GAGAAACCTAAGAAGCTTTT+GGG + chr4.2:72188585-72188604 MS.gene48648:CDS 35.0%
! TGCATTTGTTTGTTGCTGTT+TGG - chr4.2:72188562-72188581 None:intergenic 35.0%
!! AAAGCTTGCTTTTGTAGCTT+CGG - chr4.2:72188849-72188868 None:intergenic 35.0%
!!! GCTGATTTGCTCAATTTTCA+AGG + chr4.2:72188618-72188637 MS.gene48648:CDS 35.0%
!!! GTTTTGGTGTTTCCTAAGAT+TGG - chr4.2:72188372-72188391 None:intergenic 35.0%
!!! TGTTTGGACTAGCTTTTACA+AGG + chr4.2:72188785-72188804 MS.gene48648:intron 35.0%
!!! TTTCTGAGGCTGTTTTTAGA+GGG - chr4.2:72189428-72189447 None:intergenic 35.0%
!!! TTTTCTGAGGCTGTTTTTAG+AGG - chr4.2:72189429-72189448 None:intergenic 35.0%
ACAGCAACAAACAAATGCAG+AGG + chr4.2:72188563-72188582 MS.gene48648:CDS 40.0%
ACTTGCAACTTCTTGAGCAA+GGG - chr4.2:72189520-72189539 None:intergenic 40.0%
ATTGCTTCTACATCAGCTTC+TGG - chr4.2:72189351-72189370 None:intergenic 40.0%
CAAACTGTAGTGAGAGTTTG+AGG - chr4.2:72188430-72188449 None:intergenic 40.0%
CTTCATCATCATTCTCCCAA+TGG - chr4.2:72188521-72188540 None:intergenic 40.0%
GAGTTGACACAAGAATCCAT+TGG + chr4.2:72188502-72188521 MS.gene48648:CDS 40.0%
GCAAGTTCATGCTTCTACTA+AGG + chr4.2:72188449-72188468 MS.gene48648:CDS 40.0%
TCTTCACCCAAAAGCTTCTT+AGG - chr4.2:72188594-72188613 None:intergenic 40.0%
TTATGAACCTGTTCCAGACA+TGG + chr4.2:72189599-72189618 MS.gene48648:CDS 40.0%
TTCTTCAAGGCTACCTCATA+AGG - chr4.2:72189579-72189598 None:intergenic 40.0%
! CATTGGGAGAATGATGATGA+AGG + chr4.2:72188519-72188538 MS.gene48648:CDS 40.0%
! GGAGAAACCTAAGAAGCTTT+TGG + chr4.2:72188584-72188603 MS.gene48648:CDS 40.0%
!! AAGCTTGCTTTTGTAGCTTC+GGG - chr4.2:72188848-72188867 None:intergenic 40.0%
!! TTTTCACGTATAGCTTGCCT+CGG - chr4.2:72188824-72188843 None:intergenic 40.0%
!!! TAGTGCTCTTTTGGTTTAGC+CGG - chr4.2:72188912-72188931 None:intergenic 40.0%
ACATGGTTGATCGTCTTGGT+GGG + chr4.2:72189616-72189635 MS.gene48648:CDS 45.0%
ATTCTTGTGTCAACTCCAGG+TGG - chr4.2:72188498-72188517 None:intergenic 45.0%
GACTTGCAACTTCTTGAGCA+AGG - chr4.2:72189521-72189540 None:intergenic 45.0%
GCTTCTACTAAGGACACTCA+AGG + chr4.2:72188459-72188478 MS.gene48648:CDS 45.0%
TGGATTCTTGTGTCAACTCC+AGG - chr4.2:72188501-72188520 None:intergenic 45.0%
! GATGATGAAGGTTGGATTGG+AGG + chr4.2:72188531-72188550 MS.gene48648:CDS 45.0%
! GGGAGAATGATGATGAAGGT+TGG + chr4.2:72188523-72188542 MS.gene48648:CDS 45.0%
! TTTCACGTATAGCTTGCCTC+GGG - chr4.2:72188823-72188842 None:intergenic 45.0%
AAGGCAAATATCACGTCCCG+AGG + chr4.2:72188804-72188823 MS.gene48648:intron 50.0%
AGAGGGAGTGAAGTTGTGTC+TGG - chr4.2:72189411-72189430 None:intergenic 50.0%
CCAAGACGATCAACCATGTC+TGG - chr4.2:72189615-72189634 None:intergenic 50.0%
CCAGACATGGTTGATCGTCT+TGG + chr4.2:72189612-72189631 MS.gene48648:CDS 50.0%
GACATGGTTGATCGTCTTGG+TGG + chr4.2:72189615-72189634 MS.gene48648:CDS 50.0%
! CGATCAACCATGTCTGGAAC+AGG - chr4.2:72189609-72189628 None:intergenic 50.0%
!! ACTCCAGGTGGTGCTTTTTG+TGG - chr4.2:72188486-72188505 None:intergenic 50.0%
!!! TGCTCTTTTGGTTTAGCCGG+TGG - chr4.2:72188909-72188928 None:intergenic 50.0%
!! GGACCACAAAAAGCACCACC+TGG + chr4.2:72188480-72188499 MS.gene48648:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 72188324 72189749 72188324 ID=MS.gene48648
chr4.2 mRNA 72188324 72189749 72188324 ID=MS.gene48648.t1;Parent=MS.gene48648
chr4.2 exon 72188324 72188652 72188324 ID=MS.gene48648.t1.exon1;Parent=MS.gene48648.t1
chr4.2 CDS 72188324 72188652 72188324 ID=cds.MS.gene48648.t1;Parent=MS.gene48648.t1
chr4.2 exon 72189332 72189749 72189332 ID=MS.gene48648.t1.exon2;Parent=MS.gene48648.t1
chr4.2 CDS 72189332 72189749 72189332 ID=cds.MS.gene48648.t1;Parent=MS.gene48648.t1
Gene Sequence

>MS.gene48648

ATGGCTTCAACAGCAACACCTTCTCCACAAATTCTACTATTCAATCCAATCTTAGGAAACACCAAAACAATAAGAAGAATAAGATTTTTTAGGATAACAACAACCTCAAACTCTCACTACAGTTTGCAAGTTCATGCTTCTACTAAGGACACTCAAGGACCACAAAAAGCACCACCTGGAGTTGACACAAGAATCCATTGGGAGAATGATGATGAAGGTTGGATTGGAGGTAGCACCAAACAGCAACAAACAAATGCAGAGGAGAAACCTAAGAAGCTTTTGGGTGAAGACTTTGCTGATTTGCTCAATTTTCAAGGTTCTCATTATGAGTGAGTTTCTTTATAACCGTTTCCTACAAAAAGTATAAAACACCAAAAACCACTTCGATTTTCTATTAGGTCCGATTGTAAACTGAATTTTGCCTTGAAATTCAATTTTAAACTAAAGTTCGTATAAGTATGTGTTTGGACTAGCTTTTACAAGGCAAATATCACGTCCCGAGGCAAGCTATACGTGAAAATCCCGAAGCTACAAAAGCAAGCTTTTGTAAAAACGTGAAAATTTACCGTGATTTTATAAAAACCACCGGCTAAACCAAAAGAGCACTAAGTATAACTAAAGCATTTCAGCTAATGTTGAATAGTTTGGATACGACTTGAGTTCAATTGTTGGCTAAAAGACTAAAACAACTATTAGTCGGACTTTACTTCCTTTTTGATCAAACTCTGACATAATCGGTACATGTTTTGGGTTAAGATAAAAATATTAAGAGTTTGTTGCATTGTAAACTGATTTTTTCAGAATGAAGTTCATTTAAACTTAAGTTTAGTCAAGATACTATTATTAAATTCTGTTTTTCTTCAGGTAACATGAGAATTTTCTTTTCGTTTTAAAAAAAGAAAAACTGGTGCATAAGTTGCCAACTTACTAGTTACCAGAGATTTGTATCATGAGAAAACTTGTTTATTTAAATGACAAGAGTTACTGAATAATGGAATATTTGTGCAGATTCTTAGGAATATCACCAGAAGCTGATGTAGAAGCAATCAAAGTAGCATTCAGGAAACTTTCAAAGGAGTATCATCCAGACACAACTTCACTCCCTCTAAAAACAGCCTCAGAAAAATTCATGAAATTAAAAGAAGTTTACACTGTTCTGAGCAATGAAGAAAGTAGAAGATTCTATGATTGGACCCTTGCTCAAGAAGTTGCAAGTCGCCAACAAGAGAAACTGAAAATAAAATTAGAGGATCCTTATGAGGTAGCCTTGAAGAATTATGAACCTGTTCCAGACATGGTTGATCGTCTTGGTGGGAAGAATATGAAGCTTAGTGATCAAGCTGTTTCTGCTATCACTATTGATATTTTTATCATTATTTTTTCCATATGCTGTATCACTCATGTAGTTTTCTTTAAAGAATACTGA

Protein sequence

>MS.gene48648.t1

MASTATPSPQILLFNPILGNTKTIRRIRFFRITTTSNSHYSLQVHASTKDTQGPQKAPPGVDTRIHWENDDEGWIGGSTKQQQTNAEEKPKKLLGEDFADLLNFQGSHYEFLGISPEADVEAIKVAFRKLSKEYHPDTTSLPLKTASEKFMKLKEVYTVLSNEESRRFYDWTLAQEVASRQQEKLKIKLEDPYEVALKNYEPVPDMVDRLGGKNMKLSDQAVSAITIDIFIIIFSICCITHVVFFKEY