AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93247


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93247.t1 MTR_7g444820 95.349 86 4 0 1 86 61 146 6.75e-54 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93247.t1 AT4G00030 80.233 86 17 0 1 86 70 155 5.35e-45 144

Find 21 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGAGAAGGCTGGCTTGTT+TGG 0.287312 7.4:+58025903 MS.gene93247:CDS
CGGCAACTTGGCGGTTGCTA+TGG 0.431789 7.4:+58025853 MS.gene93247:CDS
ATCAATGCAATGGCCGAGCT+TGG 0.432490 7.4:+58025798 None:intergenic
GGCTTGTTTGGAACTAAAGC+TGG 0.432776 7.4:+58025915 MS.gene93247:CDS
GTCGAATATTGAAGTTGCTT+CGG 0.436707 7.4:+58026022 MS.gene93247:CDS
TACTGTACCGGTGCCAAGCT+CGG 0.464081 7.4:-58025811 None:intergenic
CTGTTCATCATTGAGAAGGC+TGG 0.471690 7.4:+58025894 MS.gene93247:CDS
ATTCCCTGTCGGCAACTTGG+CGG 0.514923 7.4:+58025844 MS.gene93247:CDS
GTGATTCCCTGTCGGCAACT+TGG 0.515452 7.4:+58025841 MS.gene93247:CDS
GCAACCGCCAAGTTGCCGAC+AGG 0.517472 7.4:-58025848 None:intergenic
CGAACAAAGAAAACACCATC+AGG 0.525042 7.4:-58025999 None:intergenic
AGTTGCCGACAGGGAATCAC+CGG 0.537341 7.4:-58025838 None:intergenic
ATCACCGGTGGTTACTGTAC+CGG 0.542477 7.4:-58025823 None:intergenic
CAACCGCCAAGTTGCCGACA+GGG 0.563353 7.4:-58025847 None:intergenic
GCAATGGCCGAGCTTGGCAC+CGG 0.566613 7.4:+58025804 None:intergenic
GCAGCTGTTCATCATTGAGA+AGG 0.611613 7.4:+58025890 MS.gene93247:CDS
GTTGCTATGGACTACTGAGA+AGG 0.629470 7.4:+58025866 MS.gene93247:CDS
GGCACCGGTACAGTAACCAC+CGG 0.635581 7.4:+58025819 MS.gene93247:CDS
AACCACCGGTGATTCCCTGT+CGG 0.657904 7.4:+58025833 MS.gene93247:CDS
TGCCGACAGGGAATCACCGG+TGG 0.664133 7.4:-58025835 None:intergenic
ACAAAGAAAACACCATCAGG+AGG 0.672815 7.4:-58025996 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GTCGAATATTGAAGTTGCTT+CGG + chr7.4:58026022-58026041 MS.gene93247:CDS 35.0%
! TAAAGCTGGAGATGTTTTAC+AGG + chr7.4:58025929-58025948 MS.gene93247:CDS 35.0%
!! CTGATGGTGTTTTCTTTGTT+CGG + chr7.4:58026000-58026019 MS.gene93247:CDS 35.0%
!! GTTATTACTTTTCCTCCTGA+TGG + chr7.4:58025984-58026003 MS.gene93247:CDS 35.0%
ACAAAGAAAACACCATCAGG+AGG - chr7.4:58025999-58026018 None:intergenic 40.0%
CGAACAAAGAAAACACCATC+AGG - chr7.4:58026002-58026021 None:intergenic 40.0%
CTGTTCATCATTGAGAAGGC+TGG + chr7.4:58025894-58025913 MS.gene93247:CDS 45.0%
GCAGCTGTTCATCATTGAGA+AGG + chr7.4:58025890-58025909 MS.gene93247:CDS 45.0%
! GGCTTGTTTGGAACTAAAGC+TGG + chr7.4:58025915-58025934 MS.gene93247:CDS 45.0%
! GTTGCTATGGACTACTGAGA+AGG + chr7.4:58025866-58025885 MS.gene93247:CDS 45.0%
!! ATTGAGAAGGCTGGCTTGTT+TGG + chr7.4:58025903-58025922 MS.gene93247:CDS 45.0%
ATCACCGGTGGTTACTGTAC+CGG - chr7.4:58025826-58025845 None:intergenic 50.0%
AACCACCGGTGATTCCCTGT+CGG + chr7.4:58025833-58025852 MS.gene93247:CDS 55.0%
AGTTGCCGACAGGGAATCAC+CGG - chr7.4:58025841-58025860 None:intergenic 55.0%
ATTCCCTGTCGGCAACTTGG+CGG + chr7.4:58025844-58025863 MS.gene93247:CDS 55.0%
GTGATTCCCTGTCGGCAACT+TGG + chr7.4:58025841-58025860 MS.gene93247:CDS 55.0%
! TACTGTACCGGTGCCAAGCT+CGG - chr7.4:58025814-58025833 None:intergenic 55.0%
CAACCGCCAAGTTGCCGACA+GGG - chr7.4:58025850-58025869 None:intergenic 60.0%
CGGCAACTTGGCGGTTGCTA+TGG + chr7.4:58025853-58025872 MS.gene93247:CDS 60.0%
!! GGCACCGGTACAGTAACCAC+CGG + chr7.4:58025819-58025838 MS.gene93247:CDS 60.0%
GCAACCGCCAAGTTGCCGAC+AGG - chr7.4:58025851-58025870 None:intergenic 65.0%
TGCCGACAGGGAATCACCGG+TGG - chr7.4:58025838-58025857 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 58025807 58026067 58025807 ID=MS.gene93247
chr7.4 mRNA 58025807 58026067 58025807 ID=MS.gene93247.t1;Parent=MS.gene93247
chr7.4 exon 58025807 58026067 58025807 ID=MS.gene93247.t1.exon1;Parent=MS.gene93247.t1
chr7.4 CDS 58025807 58026067 58025807 ID=cds.MS.gene93247.t1;Parent=MS.gene93247.t1
Gene Sequence

>MS.gene93247

ATGGCCGAGCTTGGCACCGGTACAGTAACCACCGGTGATTCCCTGTCGGCAACTTGGCGGTTGCTATGGACTACTGAGAAGGAGCAGCTGTTCATCATTGAGAAGGCTGGCTTGTTTGGAACTAAAGCTGGAGATGTTTTACAGGTCATTGACGTTAAGAATTTGATTCTTAACAATGTTATTACTTTTCCTCCTGATGGTGTTTTCTTTGTTCGGTCGAATATTGAAGTTGCTTCGGAGCAGAGAGTGAACTTCAAGTGA

Protein sequence

>MS.gene93247.t1

MAELGTGTVTTGDSLSATWRLLWTTEKEQLFIIEKAGLFGTKAGDVLQVIDVKNLILNNVITFPPDGVFFVRSNIEVASEQRVNFK