AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007322.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007322.01.T01 MTR_2g020820 97.382 191 5 0 1 191 1 191 1.27e-128 365
MsG0280007322.01.T01 MTR_4g118510 63.874 191 68 1 2 192 1 190 2.74e-68 210
MsG0280007322.01.T01 MTR_4g118510 63.874 191 68 1 2 192 1 190 1.10e-67 210
MsG0280007322.01.T01 MTR_4g118510 61.780 191 67 2 2 192 1 185 3.45e-63 197
MsG0280007322.01.T01 MTR_4g118510 61.257 191 67 2 2 192 1 184 7.47e-62 195
MsG0280007322.01.T01 MTR_0260s0030 47.541 122 64 0 10 131 22 143 5.79e-31 114
MsG0280007322.01.T01 MTR_0260s0030 47.541 122 64 0 10 131 22 143 2.97e-30 113
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007322.01.T01 AT4G15545 62.121 132 49 1 6 136 16 147 9.00e-46 154
MsG0280007322.01.T01 AT1G56080 44.800 125 69 0 7 131 4 128 1.56e-29 111
MsG0280007322.01.T01 AT1G16520 46.825 126 66 1 10 135 7 131 6.44e-29 110

Find 46 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAGCAAGTGCAAGCTTAT+CGG 0.335011 2:+13596564 None:intergenic
CATCGCAGAGCTGGAAGCTC+AGG 0.340681 2:-13596620 MsG0280007322.01.T01:CDS
ATTTCCTGCATCAGAAGTAT+TGG 0.424653 2:+13595545 None:intergenic
TGCAGCTATGTTACATAGTC+AGG 0.425161 2:-13595749 MsG0280007322.01.T01:CDS
GTTATAGGAGAAGTTGTTGA+AGG 0.435966 2:-13596420 MsG0280007322.01.T01:intron
AGACGAGCTCATCGCAGAGC+TGG 0.457689 2:-13596629 MsG0280007322.01.T01:CDS
GTACAGTGAGGAAGCTCAGT+AGG 0.476497 2:-13596379 MsG0280007322.01.T01:CDS
GGAGAGGAATGCAGAAGCAT+TGG 0.489445 2:+13596932 None:intergenic
GTTTGCATTGAAGAAGGTCT+AGG 0.497927 2:+13595567 None:intergenic
TGCACTTGCTGAACAAGATA+AGG 0.510112 2:-13596554 MsG0280007322.01.T01:intron
GTATTGGTTTGCATTGAAGA+AGG 0.512535 2:+13595561 None:intergenic
AGAACTTGCAGCAACTCCTC+GGG 0.515170 2:+13596772 None:intergenic
CAAACCAATACTTCTGATGC+AGG 0.521825 2:-13595549 MsG0280007322.01.T01:CDS
CGTGTTGACACGTGTTGAAA+GGG 0.523596 2:+13596696 None:intergenic
GTAGAGCAGAAACTTCTGAT+TGG 0.533919 2:+13596671 None:intergenic
TAGAACTTGCAGCAACTCCT+CGG 0.534065 2:+13596771 None:intergenic
GCCACATCGAGTTGCTCGAA+AGG 0.555075 2:+13596739 None:intergenic
ACCACTTCCACATCTCAGCT+TGG 0.564003 2:-13595721 MsG0280007322.01.T01:CDS
ACTTCCACATCTCAGCTTGG+AGG 0.564335 2:-13595718 MsG0280007322.01.T01:intron
TGTAACATAGCTGCAATGTT+GGG 0.565145 2:+13595757 None:intergenic
ATGTAACATAGCTGCAATGT+TGG 0.581127 2:+13595756 None:intergenic
GCTCAGTAGGGATGTCTCCA+AGG 0.581456 2:-13596366 MsG0280007322.01.T01:intron
GCGTGTTGACACGTGTTGAA+AGG 0.583885 2:+13596695 None:intergenic
TACAGTGAGGAAGCTCAGTA+GGG 0.584079 2:-13596378 MsG0280007322.01.T01:CDS
TCCAAGCTGAGATGTGGAAG+TGG 0.584301 2:+13595720 None:intergenic
ATCGAGTTGCTCGAAAGGAT+CGG 0.586114 2:+13596744 None:intergenic
TGCAGGAAATTCCTTTGCAG+AGG 0.596634 2:-13595532 MsG0280007322.01.T01:CDS
TCCTTTCGAGCAACTCGATG+TGG 0.598793 2:-13596740 MsG0280007322.01.T01:CDS
AGTACCTCCAAGCTGAGATG+TGG 0.600275 2:+13595714 None:intergenic
AGAGAAGAACATGTTGGTGG+CGG 0.608636 2:-13596821 MsG0280007322.01.T01:exon
GCAGAGGATCGTCAATCAGA+TGG 0.614191 2:-13595516 MsG0280007322.01.T01:CDS
ACTTATGCAATCACTACAAG+AGG 0.615603 2:-13596224 MsG0280007322.01.T01:CDS
ATCGCAGAGCTGGAAGCTCA+GGG 0.620221 2:-13596619 MsG0280007322.01.T01:CDS
AAGAGAGAAGAACATGTTGG+TGG 0.623218 2:-13596824 MsG0280007322.01.T01:exon
TGATTGACGATCCTCTGCAA+AGG 0.623637 2:+13595521 None:intergenic
CAGCTTCAGAAAGAGCAGCA+TGG 0.627433 2:+13596587 None:intergenic
GACACGTGTTGAAAGGGCAA+TGG 0.633302 2:+13596702 None:intergenic
TCAGAAAGAGCAGCATGGAG+AGG 0.647214 2:+13596592 None:intergenic
ACGTGTTGAAAGGGCAATGG+AGG 0.665793 2:+13596705 None:intergenic
TGCATTGAAGAAGGTCTAGG+TGG 0.671228 2:+13595570 None:intergenic
GCAACTCCTCGGGAATATCG+AGG 0.680265 2:+13596782 None:intergenic
ATTGGAACGTGATAAGACAA+TGG 0.689309 2:+13596950 None:intergenic
TTCAAACCTCGATATTCCCG+AGG 0.693018 2:-13596788 MsG0280007322.01.T01:CDS
AGTTGCTCGAAAGGATCGGA+CGG 0.696761 2:+13596748 None:intergenic
ATGTTGGTGGCGGAATTGAG+TGG 0.699161 2:-13596811 MsG0280007322.01.T01:CDS
CTTCACTTTCTAGTACAGTG+AGG 0.701338 2:-13596391 MsG0280007322.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAATTATATGTAATTAATTT+TGG + Chr2:13596851-13596870 None:intergenic 10.0%
!! AAATATTAATAACAACTTAC+AGG + Chr2:13596315-13596334 None:intergenic 15.0%
!! ATTAATTAAACCAAAACTAA+TGG + Chr2:13596575-13596594 None:intergenic 15.0%
!! TAATTAATAAAAATTAACCC+TGG - Chr2:13596587-13596606 MsG0280007322.01.T01:CDS 15.0%
!!! ATTTGCATGAAATTTTTATT+TGG - Chr2:13596008-13596027 MsG0280007322.01.T01:intron 15.0%
!!! CAATTAAGCATTTTAAAAAA+TGG + Chr2:13596619-13596638 None:intergenic 15.0%
!!! TTAATTAATTCTTGTTATAC+TGG - Chr2:13596214-13596233 MsG0280007322.01.T01:CDS 15.0%
!!! TTATGATTTTATTCATAGTT+TGG + Chr2:13595594-13595613 None:intergenic 15.0%
!!! TTTTTCTACTTTCTTTTAAT+TGG + Chr2:13595657-13595676 None:intergenic 15.0%
!! AAAACAAAAAAGATAGATGT+AGG - Chr2:13595518-13595537 MsG0280007322.01.T01:CDS 20.0%
!! AAACAAAAAAGATAGATGTA+GGG - Chr2:13595519-13595538 MsG0280007322.01.T01:CDS 20.0%
!! ATGAGAACTAAATAAATCAA+AGG + Chr2:13596885-13596904 None:intergenic 20.0%
!! ATTCAAATATAGTTAACAAG+AGG - Chr2:13596674-13596693 MsG0280007322.01.T01:CDS 20.0%
!! GAGATTATGAAAATTGTTAT+AGG - Chr2:13596067-13596086 MsG0280007322.01.T01:intron 20.0%
!! TGAGAACTAAATAAATCAAA+GGG + Chr2:13596884-13596903 None:intergenic 20.0%
!!! AATTTTCATTGTAGTAAATG+TGG - Chr2:13596191-13596210 MsG0280007322.01.T01:intron 20.0%
!!! GGATTAATATTTTTCAATAG+AGG - Chr2:13596367-13596386 MsG0280007322.01.T01:CDS 20.0%
!!! TTTTAATATGAATATGCAGT+TGG - Chr2:13596239-13596258 MsG0280007322.01.T01:CDS 20.0%
! GAAAAATATTAATCCAGACA+AGG + Chr2:13596362-13596381 None:intergenic 25.0%
!! ATTTTATTCATAGTTTGGAG+AGG + Chr2:13595589-13595608 None:intergenic 25.0%
!! GTACTAAGTTCAAATTTTCT+TGG - Chr2:13596532-13596551 MsG0280007322.01.T01:intron 25.0%
!! TGGATCATTTTCTTTTCATT+TGG + Chr2:13596831-13596850 None:intergenic 25.0%
!!! AGCATTTTAAAAAATGGAGA+AGG + Chr2:13596613-13596632 None:intergenic 25.0%
!!! ATGCAATTACTTTTTTCAGT+AGG - Chr2:13596424-13596443 MsG0280007322.01.T01:intron 25.0%
!!! TGCAATTACTTTTTTCAGTA+GGG - Chr2:13596425-13596444 MsG0280007322.01.T01:intron 25.0%
!!! TTTGATTTTGACATGTAACA+TGG - Chr2:13596698-13596717 MsG0280007322.01.T01:CDS 25.0%
AAAAAGAGAGAAGAACATGT+TGG - Chr2:13595675-13595694 MsG0280007322.01.T01:intron 30.0%
TAAATCAAAGGGTTTATGTG+AGG + Chr2:13596873-13596892 None:intergenic 30.0%
TAATATGAATATGCAGTTGG+AGG - Chr2:13596242-13596261 MsG0280007322.01.T01:CDS 30.0%
! AAACCAAATAATTTGCACCT+TGG + Chr2:13596156-13596175 None:intergenic 30.0%
! TTGACATGTAACATGGTTTT+TGG - Chr2:13596705-13596724 MsG0280007322.01.T01:CDS 30.0%
!! CGTAAAATGACCATTAGTTT+TGG - Chr2:13596562-13596581 MsG0280007322.01.T01:CDS 30.0%
ACCACTTTCTAAACCAAACT+CGG - Chr2:13595981-13596000 MsG0280007322.01.T01:intron 35.0%
ACTTATGCAATCACTACAAG+AGG - Chr2:13596278-13596297 MsG0280007322.01.T01:intron 35.0%
ATACTGTTTATGACCTTGTC+TGG - Chr2:13596346-13596365 MsG0280007322.01.T01:intron 35.0%
ATGTAACATAGCTGCAATGT+TGG + Chr2:13596749-13596768 None:intergenic 35.0%
ATTGGAACGTGATAAGACAA+TGG + Chr2:13595555-13595574 None:intergenic 35.0%
GTTATAGGAGAAGTTGTTGA+AGG - Chr2:13596082-13596101 MsG0280007322.01.T01:intron 35.0%
TCTCCAAGGTGCAAATTATT+TGG - Chr2:13596150-13596169 MsG0280007322.01.T01:intron 35.0%
TGTAACATAGCTGCAATGTT+GGG + Chr2:13596748-13596767 None:intergenic 35.0%
TTAAAAAATGGAGAAGGCCA+GGG + Chr2:13596607-13596626 None:intergenic 35.0%
TTTAAAAAATGGAGAAGGCC+AGG + Chr2:13596608-13596627 None:intergenic 35.0%
! ATTTCCTGCATCAGAAGTAT+TGG + Chr2:13596960-13596979 None:intergenic 35.0%
! GTTTGGTTTAGAAAGTGGTT+CGG + Chr2:13595980-13595999 None:intergenic 35.0%
!! GTATTGGTTTGCATTGAAGA+AGG + Chr2:13596944-13596963 None:intergenic 35.0%
AAGAGAGAAGAACATGTTGG+TGG - Chr2:13595678-13595697 MsG0280007322.01.T01:intron 40.0%
CAAACCAATACTTCTGATGC+AGG - Chr2:13596953-13596972 MsG0280007322.01.T01:five_prime_UTR 40.0%
CATGCAAATTGAACCGAGTT+TGG + Chr2:13595997-13596016 None:intergenic 40.0%
GTAGAGCAGAAACTTCTGAT+TGG + Chr2:13595834-13595853 None:intergenic 40.0%
GTTTGCATTGAAGAAGGTCT+AGG + Chr2:13596938-13596957 None:intergenic 40.0%
TGCAGCTATGTTACATAGTC+AGG - Chr2:13596753-13596772 MsG0280007322.01.T01:CDS 40.0%
TTCAGCAAGTGCAAGCTTAT+CGG + Chr2:13595941-13595960 None:intergenic 40.0%
! ACCGAGTTTGGTTTAGAAAG+TGG + Chr2:13595985-13596004 None:intergenic 40.0%
! CTTCACTTTCTAGTACAGTG+AGG - Chr2:13596111-13596130 MsG0280007322.01.T01:intron 40.0%
! TGCACTTGCTGAACAAGATA+AGG - Chr2:13595948-13595967 MsG0280007322.01.T01:intron 40.0%
!!! GTAACATGGTTTTTGGTGCA+GGG - Chr2:13596712-13596731 MsG0280007322.01.T01:CDS 40.0%
!!! TGTAACATGGTTTTTGGTGC+AGG - Chr2:13596711-13596730 MsG0280007322.01.T01:CDS 40.0%
TACAGTGAGGAAGCTCAGTA+GGG - Chr2:13596124-13596143 MsG0280007322.01.T01:intron 45.0%
TAGAACTTGCAGCAACTCCT+CGG + Chr2:13595734-13595753 None:intergenic 45.0%
TGATTGACGATCCTCTGCAA+AGG + Chr2:13596984-13597003 None:intergenic 45.0%
TGCAGGAAATTCCTTTGCAG+AGG - Chr2:13596970-13596989 MsG0280007322.01.T01:five_prime_UTR 45.0%
TGCATTGAAGAAGGTCTAGG+TGG + Chr2:13596935-13596954 None:intergenic 45.0%
TTCAAACCTCGATATTCCCG+AGG - Chr2:13595714-13595733 MsG0280007322.01.T01:intron 45.0%
! AGAGAAGAACATGTTGGTGG+CGG - Chr2:13595681-13595700 MsG0280007322.01.T01:intron 45.0%
! ATCGAGTTGCTCGAAAGGAT+CGG + Chr2:13595761-13595780 None:intergenic 45.0%
! CGTGTTGACACGTGTTGAAA+GGG + Chr2:13595809-13595828 None:intergenic 45.0%
! GCAGTTGGAGGTTTTCAGAA+AGG - Chr2:13596254-13596273 MsG0280007322.01.T01:intron 45.0%
!! AATATAATAATTACAATATT+TGG - Chr2:13596499-13596518 MsG0280007322.01.T01:intron 5.0%
ACCACTTCCACATCTCAGCT+TGG - Chr2:13596781-13596800 MsG0280007322.01.T01:CDS 50.0%
ACGTGTTGAAAGGGCAATGG+AGG + Chr2:13595800-13595819 None:intergenic 50.0%
ACTTCCACATCTCAGCTTGG+AGG - Chr2:13596784-13596803 MsG0280007322.01.T01:CDS 50.0%
AGAACTTGCAGCAACTCCTC+GGG + Chr2:13595733-13595752 None:intergenic 50.0%
AGTACCTCCAAGCTGAGATG+TGG + Chr2:13596791-13596810 None:intergenic 50.0%
AGTTGCTCGAAAGGATCGGA+CGG + Chr2:13595757-13595776 None:intergenic 50.0%
CAGCTTCAGAAAGAGCAGCA+TGG + Chr2:13595918-13595937 None:intergenic 50.0%
GACACGTGTTGAAAGGGCAA+TGG + Chr2:13595803-13595822 None:intergenic 50.0%
GCAGAGGATCGTCAATCAGA+TGG - Chr2:13596986-13597005 MsG0280007322.01.T01:five_prime_UTR 50.0%
GTACAGTGAGGAAGCTCAGT+AGG - Chr2:13596123-13596142 MsG0280007322.01.T01:intron 50.0%
TCAGAAAGAGCAGCATGGAG+AGG + Chr2:13595913-13595932 None:intergenic 50.0%
TCCAAGCTGAGATGTGGAAG+TGG + Chr2:13596785-13596804 None:intergenic 50.0%
TCCTTTCGAGCAACTCGATG+TGG - Chr2:13595762-13595781 MsG0280007322.01.T01:CDS 50.0%
! GCGTGTTGACACGTGTTGAA+AGG + Chr2:13595810-13595829 None:intergenic 50.0%
! GGAGAGGAATGCAGAAGCAT+TGG + Chr2:13595573-13595592 None:intergenic 50.0%
!! ATGTTGGTGGCGGAATTGAG+TGG - Chr2:13595691-13595710 MsG0280007322.01.T01:intron 50.0%
ATCGCAGAGCTGGAAGCTCA+GGG - Chr2:13595883-13595902 MsG0280007322.01.T01:intron 55.0%
GCAACTCCTCGGGAATATCG+AGG + Chr2:13595723-13595742 None:intergenic 55.0%
GCTCAGTAGGGATGTCTCCA+AGG - Chr2:13596136-13596155 MsG0280007322.01.T01:intron 55.0%
! GCCACATCGAGTTGCTCGAA+AGG + Chr2:13595766-13595785 None:intergenic 55.0%
!!! TGGTTTTTGGTGCAGGGAGC+AGG - Chr2:13596718-13596737 MsG0280007322.01.T01:CDS 55.0%
AGACGAGCTCATCGCAGAGC+TGG - Chr2:13595873-13595892 MsG0280007322.01.T01:intron 60.0%
CATCGCAGAGCTGGAAGCTC+AGG - Chr2:13595882-13595901 MsG0280007322.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 13595506 13597018 13595506 ID=MsG0280007322.01;Name=MsG0280007322.01
Chr2 mRNA 13595506 13597018 13595506 ID=MsG0280007322.01.T01;Parent=MsG0280007322.01;Name=MsG0280007322.01.T01;_AED=0.49;_eAED=0.50;_QI=185|1|0.8|1|1|1|5|0|193
Chr2 exon 13595506 13595609 13595506 ID=MsG0280007322.01.T01:exon:5761;Parent=MsG0280007322.01.T01
Chr2 exon 13595719 13595791 13595719 ID=MsG0280007322.01.T01:exon:5760;Parent=MsG0280007322.01.T01
Chr2 exon 13596210 13596266 13596210 ID=MsG0280007322.01.T01:exon:5759;Parent=MsG0280007322.01.T01
Chr2 exon 13596367 13596435 13596367 ID=MsG0280007322.01.T01:exon:5758;Parent=MsG0280007322.01.T01
Chr2 exon 13596555 13597018 13596555 ID=MsG0280007322.01.T01:exon:5757;Parent=MsG0280007322.01.T01
Chr2 five_prime_UTR 13596834 13597018 13596834 ID=MsG0280007322.01.T01:five_prime_utr;Parent=MsG0280007322.01.T01
Chr2 CDS 13596555 13596833 13596555 ID=MsG0280007322.01.T01:cds;Parent=MsG0280007322.01.T01
Chr2 CDS 13596367 13596435 13596367 ID=MsG0280007322.01.T01:cds;Parent=MsG0280007322.01.T01
Chr2 CDS 13596210 13596266 13596210 ID=MsG0280007322.01.T01:cds;Parent=MsG0280007322.01.T01
Chr2 CDS 13595719 13595791 13595719 ID=MsG0280007322.01.T01:cds;Parent=MsG0280007322.01.T01
Chr2 CDS 13595506 13595609 13595506 ID=MsG0280007322.01.T01:cds;Parent=MsG0280007322.01.T01
Gene Sequence

>MsG0280007322.01.T01

ATGTTGGTGGCGGAATTGAGTGGTTCAAACCTCGATATTCCCGAGGAGTTGCTGCAAGTTCTACCGTCCGATCCTTTCGAGCAACTCGATGTGGCTCGCAAAATCACCTCCATTGCCCTTTCAACACGTGTCAACACGCTCCAATCAGAAGTTTCTGCTCTACGTGATGAGCTTGTCAAGAAAGACGAGCTCATCGCAGAGCTGGAAGCTCAGGGAGAGCCTCTCCATGCTGCTCTTTCTGAAGCTGCCGATAAGCTTGCACTTGCTGAACAAGATAAGGAGAAGTTGTTGAAGGAGAATGCTTCACTTTCTAGTACAGTGAGGAAGCTCAGTAGGGATGTCTCCAAGTTGGAGGTTTTCAGAAAGGCACTTATGCAATCACTACAAGAGGATGAAGAAAAACCTGGAGCAGGATCACCCAACATTGCAGCTATGTTACATAGTCAGGCAAGTACCACTTCCACATCTCAGCTTGGAGATGAAGATGCTGCATTGCCACCTAGACCTTCTTCAATGCAAACCAATACTTCTGATGCAGGAAATTCCTTTGCAGAGGATCGTCAATCAGATGGTATGTACTAA

Protein sequence

>MsG0280007322.01.T01

MLVAELSGSNLDIPEELLQVLPSDPFEQLDVARKITSIALSTRVNTLQSEVSALRDELVKKDELIAELEAQGEPLHAALSEAADKLALAEQDKEKLLKENASLSSTVRKLSRDVSKLEVFRKALMQSLQEDEEKPGAGSPNIAAMLHSQASTTSTSQLGDEDAALPPRPSSMQTNTSDAGNSFAEDRQSDGMY*