AlfalfaGEDB | Msa0188110

M. sativa cultivar zhongmu-4 / Msa0188110

KEGG: -
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464
PFAM: PF00300.25

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0188110	XP_003594381.1	96.943	229	4	2	1	229	1	226	1.00e-160	453

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0188110	sp\|Q94BY1\|Y3515_ARATH	67.598	179	56	1	51	229	38	214	1.58e-84	253

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0188110	G7ISQ0	96.943	229	4	2	1	229	1	226	4.80e-161	453

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006050	Msa0188110	0.837858	4.056209e-57	-8.615850e-47
Msa0014960	Msa0188110	0.817152	3.823707e-52	-8.615850e-47
Msa0024320	Msa0188110	0.806790	6.930720e-50	-8.615850e-47
Msa0044250	Msa0188110	0.814243	1.701607e-51	-8.615850e-47
Msa0044320	Msa0188110	0.806362	8.533855e-50	-8.615850e-47
Msa0052550	Msa0188110	0.818637	1.766762e-52	-8.615850e-47
Msa0065400	Msa0188110	0.802052	6.734937e-49	-8.615850e-47
Msa0072390	Msa0188110	0.806897	6.580229e-50	-8.615850e-47
Msa0094970	Msa0188110	0.802656	5.059250e-49	-8.615850e-47
Msa0094990	Msa0188110	0.800179	1.627541e-48	-8.615850e-47
Msa0113910	Msa0188110	0.806020	1.007584e-49	-8.615850e-47
Msa0118250	Msa0188110	0.836468	9.193885e-57	-8.615850e-47
Msa0129270	Msa0188110	-0.801250	9.837870e-49	-8.615850e-47
Msa0140330	Msa0188110	0.810911	9.106270e-51	-8.615850e-47
Msa0160470	Msa0188110	0.827991	1.152177e-54	-8.615850e-47
Msa0163400	Msa0188110	0.810130	1.343286e-50	-8.615850e-47
Msa0163960	Msa0188110	0.813455	2.537662e-51	-8.615850e-47
Msa0188110	Msa0190400	0.802714	4.921443e-49	-8.615850e-47
Msa0188110	Msa0218860	0.834415	3.036906e-56	-8.615850e-47
Msa0188110	Msa0239980	0.820020	8.552238e-53	-8.615850e-47
Msa0188110	Msa0267130	0.803683	3.101012e-49	-8.615850e-47
Msa0188110	Msa0272420	0.800429	1.447559e-48	-8.615850e-47
Msa0188110	Msa0282860	0.815549	8.732249e-52	-8.615850e-47
Msa0188110	Msa0312570	0.818149	2.278298e-52	-8.615850e-47
Msa0188110	Msa0313800	0.802028	6.811662e-49	-8.615850e-47
Msa0188110	Msa0315010	0.808865	2.510761e-50	-8.615850e-47
Msa0188110	Msa0319710	0.817643	2.964363e-52	-8.615850e-47
Msa0188110	Msa0327900	0.807000	6.259114e-50	-8.615850e-47
Msa0188110	Msa0329060	0.821060	4.933969e-53	-8.615850e-47
Msa0188110	Msa0340870	0.800288	1.545858e-48	-8.615850e-47
Msa0188110	Msa0349450	0.807439	5.050910e-50	-8.615850e-47
Msa0188110	Msa0373220	0.801423	9.069012e-49	-8.615850e-47
Msa0188110	Msa0376480	0.829652	4.566033e-55	-8.615850e-47
Msa0188110	Msa0379950	0.805594	1.237792e-49	-8.615850e-47
Msa0188110	Msa0390350	0.821567	3.770841e-53	-8.615850e-47
Msa0188110	Msa0393140	0.823841	1.114385e-53	-8.615850e-47
Msa0188110	Msa0419040	0.800128	1.666532e-48	-8.615850e-47
Msa0188110	Msa0430040	0.817783	2.756816e-52	-8.615850e-47
Msa0188110	Msa0438460	0.805013	1.638142e-49	-8.615850e-47
Msa0188110	Msa0460220	0.834227	3.386275e-56	-8.615850e-47
Msa0188110	Msa0463140	0.817973	2.497803e-52	-8.615850e-47
Msa0188110	Msa0463540	0.803554	3.298699e-49	-8.615850e-47
Msa0188110	Msa0471890	0.802437	5.613964e-49	-8.615850e-47
Msa0188110	Msa0476820	0.838536	2.712451e-57	-8.615850e-47
Msa0188110	Msa0481050	0.835228	1.896247e-56	-8.615850e-47
Msa0188110	Msa0493430	0.805539	1.271238e-49	-8.615850e-47
Msa0188110	Msa0500490	0.846091	2.700836e-59	-8.615850e-47
Msa0188110	Msa0517190	0.801720	7.881864e-49	-8.615850e-47
Msa0188110	Msa0525800	0.828079	1.097222e-54	-8.615850e-47
Msa0188110	Msa0533140	0.851833	6.858253e-61	-8.615850e-47
Msa0188110	Msa0539320	0.807663	4.529087e-50	-8.615850e-47
Msa0188110	Msa0539400	0.813507	2.471308e-51	-8.615850e-47
Msa0188110	Msa0540520	0.800344	1.506453e-48	-8.615850e-47
Msa0188110	Msa0545230	0.809823	1.563743e-50	-8.615850e-47
Msa0188110	Msa0546020	0.813808	2.121614e-51	-8.615850e-47
Msa0188110	Msa0549940	0.819403	1.183125e-52	-8.615850e-47
Msa0188110	Msa0576640	0.807260	5.514212e-50	-8.615850e-47
Msa0188110	Msa0587070	0.801980	6.971199e-49	-8.615850e-47
Msa0188110	Msa0612730	0.808668	2.765492e-50	-8.615850e-47
Msa0188110	Msa0622250	0.803335	3.662351e-49	-8.615850e-47
Msa0188110	Msa0625310	0.812298	4.548534e-51	-8.615850e-47
Msa0188110	Msa0625330	0.805794	1.123763e-49	-8.615850e-47
Msa0188110	Msa0635130	0.809700	1.662311e-50	-8.615850e-47
Msa0188110	Msa0651410	0.802355	5.836250e-49	-8.615850e-47
Msa0188110	Msa0660070	0.838737	2.407881e-57	-8.615850e-47
Msa0188110	Msa0679780	0.839740	1.322304e-57	-8.615850e-47
Msa0188110	Msa0680930	0.869170	3.783559e-66	-8.615850e-47
Msa0188110	Msa0690530	0.804889	1.739275e-49	-8.615850e-47
Msa0188110	Msa0706130	0.820078	8.295067e-53	-8.615850e-47
Msa0188110	Msa0707270	0.814388	1.580003e-51	-8.615850e-47
Msa0188110	Msa0713420	0.805687	1.183328e-49	-8.615850e-47
Msa0188110	Msa0716600	-0.807581	4.712502e-50	-8.615850e-47
Msa0188110	Msa0734760	0.832190	1.089136e-55	-8.615850e-47
Msa0188110	Msa0747900	0.801869	7.347152e-49	-8.615850e-47
Msa0188110	Msa0748050	0.800005	1.765710e-48	-8.615850e-47
Msa0188110	Msa0748330	0.806704	7.229876e-50	-8.615850e-47
Msa0188110	Msa0760950	0.803164	3.972245e-49	-8.615850e-47
Msa0188110	Msa0791430	0.819055	1.419667e-52	-8.615850e-47
Msa0188110	Msa0797010	0.811312	7.456308e-51	-8.615850e-47
Msa0188110	Msa0813630	0.868837	4.852491e-66	-8.615850e-47
Msa0188110	Msa0821030	0.813301	2.743573e-51	-8.615850e-47
Msa0188110	Msa0829110	0.844361	7.927361e-59	-8.615850e-47
Msa0188110	Msa0829430	0.808098	3.659849e-50	-8.615850e-47
Msa0188110	Msa0834690	0.814462	1.522029e-51	-8.615850e-47
Msa0188110	Msa0848780	0.808325	3.274190e-50	-8.615850e-47
Msa0188110	Msa0967670	0.815692	8.114426e-52	-8.615850e-47
Msa0188110	Msa0973380	0.807608	4.651964e-50	-8.615850e-47
Msa0188110	Msa1014120	0.803224	3.860761e-49	-8.615850e-47
Msa0188110	Msa1026610	0.807230	5.594735e-50	-8.615850e-47
Msa0188110	Msa1026880	0.805338	1.400655e-49	-8.615850e-47
Msa0188110	Msa1029430	0.801681	8.027446e-49	-8.615850e-47
Msa0188110	Msa1029510	0.853210	2.777023e-61	-8.615850e-47
Msa0188110	Msa1044790	0.862591	4.547324e-64	-8.615850e-47
Msa0188110	Msa1056590	0.805299	1.427545e-49	-8.615850e-47
Msa0188110	Msa1073140	0.805332	1.404917e-49	-8.615850e-47
Msa0188110	Msa1078600	0.818759	1.657486e-52	-8.615850e-47
Msa0188110	Msa1078770	0.813526	2.447646e-51	-8.615850e-47
Msa0188110	Msa1109500	0.837513	4.973002e-57	-8.615850e-47
Msa0188110	Msa1114240	0.804837	1.783303e-49	-8.615850e-47
Msa0188110	Msa1117280	0.803311	3.703206e-49	-8.615850e-47
Msa0188110	Msa1144420	0.867717	1.114114e-65	-8.615850e-47
Msa0188110	Msa1145930	0.810435	1.154570e-50	-8.615850e-47
Msa0188110	Msa1149570	0.812462	4.188599e-51	-8.615850e-47
Msa0188110	Msa1157310	0.807709	4.428300e-50	-8.615850e-47
Msa0188110	Msa1184010	0.800278	1.553781e-48	-8.615850e-47
Msa0188110	Msa1192150	0.827750	1.316884e-54	-8.615850e-47
Msa0188110	Msa1205520	0.816317	5.884263e-52	-8.615850e-47
Msa0188110	Msa1213550	0.816337	5.826226e-52	-8.615850e-47
Msa0188110	Msa1226880	0.826152	3.173737e-54	-8.615850e-47
Msa0188110	Msa1239000	0.820257	7.545175e-53	-8.615850e-47
Msa0188110	Msa1291140	0.817823	2.699758e-52	-8.615850e-47
Msa0188110	Msa1331400	0.813805	2.125084e-51	-8.615850e-47
Msa0188110	Msa1335500	-0.814065	1.862355e-51	-8.615850e-47
Msa0188110	Msa1336080	0.826370	2.815866e-54	-8.615850e-47
Msa0188110	Msa1341790	0.862562	4.642362e-64	-8.615850e-47
Msa0188110	Msa1342080	0.823909	1.074525e-53	-8.615850e-47
Msa0188110	Msa1349490	0.815602	8.500774e-52	-8.615850e-47
Msa0188110	Msa1349640	0.800244	1.578769e-48	-8.615850e-47
Msa0188110	Msa1364420	0.810480	1.128810e-50	-8.615850e-47
Msa0188110	Msa1369180	0.813298	2.746950e-51	-8.615850e-47
Msa0188110	Msa1372570	0.804225	2.392838e-49	-8.615850e-47
Msa0188110	Msa1372680	0.800409	1.461067e-48	-8.615850e-47
Msa0188110	Msa1378840	0.850931	1.233571e-60	-8.615850e-47
Msa0188110	Msa1384980	0.809092	2.245158e-50	-8.615850e-47
Msa0188110	Msa1386020	0.803083	4.127966e-49	-8.615850e-47
Msa0188110	Msa1393720	0.804277	2.334051e-49	-8.615850e-47
Msa0188110	Msa1405380	0.808185	3.507102e-50	-8.615850e-47
Msa0188110	Msa1431870	0.813261	2.799308e-51	-8.615850e-47
Msa0188110	Msa1432350	0.801917	7.179117e-49	-8.615850e-47
Msa0188110	Msa1435990	0.813310	2.730894e-51	-8.615850e-47
Msa0188110	Msa1451780	0.801641	8.180200e-49	-8.615850e-47
Msa0188110	Msa1463500	0.844685	6.487626e-59	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0188110	MtrunA17_Chr2g0291061	96.943	229	4	2	1	229	1	226	9.23e-165	453

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0188110	AT3G52155.1	67.598	179	56	1	51	229	38	214	1.61e-85	253
Msa0188110	AT3G52155.3	67.598	179	56	1	51	229	38	214	1.61e-85	253
Msa0188110	AT3G52155.2	69.182	159	47	1	51	209	38	194	1.06e-75	227
Msa0188110	AT3G52155.4	65.806	155	51	1	75	229	13	165	1.41e-72	218

Find 106 sgRNAs with CRISPR-Local

Find 289 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTTGGTGGGTGGAAGCTTC+AGG	0.157844	2_1:-3205029	Msa0188110:CDS
TTGGCGCGTAATCTGAAATT+AGG	0.161940	2_1:+3208574	None:intergenic
AACGCCACAAAATCTGAAAT+TGG	0.165377	2_1:-3208682	Msa0188110:five_prime_UTR
CGATCAGATTCTGTTTAATT+TGG	0.189063	2_1:-3204804	Msa0188110:three_prime_UTR
CATGTTGGCCCAACCCATTC+AGG	0.246208	2_1:+3204506	None:intergenic
GGGAATGAGGATTGAGGATT+TGG	0.289186	2_1:+3208555	None:intergenic
TTTAGCTTGGTGTCTTGTTT+GGG	0.296413	2_1:+3208535	None:intergenic
TTCGCCATGCCGAAAGTTCC+TGG	0.311015	2_1:-3208471	Msa0188110:CDS
TGAAATGAGTGTCGACATAA+TGG	0.337985	2_1:-3208756	None:intergenic
TGGCGCGTAATCTGAAATTA+GGG	0.377016	2_1:+3208575	None:intergenic
ACAGGTGCATGGGACACAAC+AGG	0.393604	2_1:-3206445	Msa0188110:intron
GAAAACTATAATTCCCTGAA+TGG	0.399507	2_1:-3204520	Msa0188110:three_prime_UTR
AACCCAACTGTTGGAGTTTG+AGG	0.406944	2_1:+3207333	None:intergenic
CATTGCTGAGAGTAAATTGT+TGG	0.408833	2_1:-3204744	Msa0188110:three_prime_UTR
GACCGGCCTCTGAGTAAGTC+AGG	0.413658	2_1:-3207380	Msa0188110:CDS
CTGACCAAACTGATGCGGTT+TGG	0.415237	2_1:+3204899	None:intergenic
CTGTTTAATTTGGCTGCAAT+GGG	0.415516	2_1:-3204794	Msa0188110:three_prime_UTR
TTTGGTGGGTGGAAGCTTCA+GGG	0.416035	2_1:-3205028	Msa0188110:CDS
ATCATCGCCGGAGCAACATT+CGG	0.422099	2_1:-3208600	Msa0188110:CDS
GGAAGAAAAGAAAGATGAAA+TGG	0.422648	2_1:+3204554	None:intergenic
GCATTTGTAGCAGCAGGGTT+TGG	0.423367	2_1:-3205046	Msa0188110:CDS
TCTGTTTAATTTGGCTGCAA+TGG	0.426680	2_1:-3204795	Msa0188110:three_prime_UTR
TTGAAACTGCTGGGAAATCT+TGG	0.430976	2_1:-3206352	Msa0188110:CDS
CAGCGATGCAGCACGGACTA+AGG	0.433131	2_1:-3207205	Msa0188110:CDS
ATCTTGACTAGCTAGCTACT+TGG	0.434900	2_1:+3204995	None:intergenic
TGGTGTCTTGTTTGGGAATG+AGG	0.437396	2_1:+3208542	None:intergenic
TTACAGGCATTTGTAGCAGC+AGG	0.439671	2_1:-3205052	Msa0188110:intron
AACTTTCGGCATGGCGAAGA+AGG	0.440447	2_1:+3208476	None:intergenic
GAAATGAGTGTCGACATAAT+GGG	0.443284	2_1:-3208755	None:intergenic
TGCGTTGTGCAGATCATGAC+CGG	0.444184	2_1:-3207397	Msa0188110:intron
ACGCCACAAAATCTGAAATT+GGG	0.444273	2_1:-3208681	Msa0188110:five_prime_UTR
AGGTTATTTGTAAATATTCA+AGG	0.448646	2_1:-3207065	Msa0188110:CDS
GGAGCAAGTGCAGGAACTAC+TGG	0.451531	2_1:-3207163	Msa0188110:CDS
GAATCCAAACCGCATCAGTT+TGG	0.456158	2_1:-3204903	Msa0188110:three_prime_UTR
TACCTAGATAGTATAAGTTC+AGG	0.461088	2_1:+3207305	None:intergenic
ACTAGGGAAAGCATACTAGA+TGG	0.463512	2_1:-3204853	Msa0188110:three_prime_UTR
TTTGTAGCAGCAGGGTTTGG+TGG	0.463635	2_1:-3205043	Msa0188110:CDS
TATTCGATTGCAGCCATGGA+TGG	0.469077	2_1:-3207111	Msa0188110:CDS
AAAACTATAATTCCCTGAAT+GGG	0.471927	2_1:-3204519	Msa0188110:three_prime_UTR
TTTCTATTCGATTGCAGCCA+TGG	0.471965	2_1:-3207115	Msa0188110:CDS
TTGTAGCAGCAGGGTTTGGT+GGG	0.472254	2_1:-3205042	Msa0188110:CDS
TCCCTCAAACTCCAACAGTT+GGG	0.472793	2_1:-3207335	Msa0188110:CDS
AGGGTGTTCCCAGGAACTTT+CGG	0.477079	2_1:+3208462	None:intergenic
GGCTTTATACCTCGGAGTGA+AGG	0.477571	2_1:+3208442	None:intergenic
TATAATTCCCTGAATGGGTT+GGG	0.480335	2_1:-3204514	Msa0188110:three_prime_UTR
GCTGCACTGCTTGAAACTGC+TGG	0.482315	2_1:-3206362	Msa0188110:CDS
ACCCAACTGTTGGAGTTTGA+GGG	0.487056	2_1:+3207334	None:intergenic
TTCCCTCAAACTCCAACAGT+TGG	0.487121	2_1:-3207336	Msa0188110:CDS
TAGCAGCAGGGTTTGGTGGG+TGG	0.487228	2_1:-3205039	Msa0188110:CDS
CAAACCGCATCAGTTTGGTC+AGG	0.494958	2_1:-3204898	Msa0188110:three_prime_UTR
CAGGTGCATGGGACACAACA+GGG	0.496705	2_1:-3206444	Msa0188110:CDS
CTTTACATTCTATACTAAGC+AGG	0.501853	2_1:+3204712	None:intergenic
AGATGCTCAAAAGATAAAGT+TGG	0.502518	2_1:-3204947	Msa0188110:three_prime_UTR
AAGGATGAGGCGGCGAGAGA+TGG	0.508749	2_1:+3208495	None:intergenic
TCAAACTCCAACAGTTGGGT+TGG	0.510018	2_1:-3207331	Msa0188110:CDS
TGCTGGGAAATCTTGGAATG+AGG	0.511570	2_1:-3206345	Msa0188110:intron
AAAGCATACTAGATGGAAGA+TGG	0.515770	2_1:-3204846	Msa0188110:three_prime_UTR
CGAGAGATGGAATCTGAGAA+AGG	0.516129	2_1:+3208508	None:intergenic
TTCCTGAACTTATACTATCT+AGG	0.516546	2_1:-3207307	Msa0188110:intron
ATGTTGGCCCAACCCATTCA+GGG	0.521865	2_1:+3204507	None:intergenic
CTATAATTCCCTGAATGGGT+TGG	0.527711	2_1:-3204515	Msa0188110:three_prime_UTR
GGCGATGATTGAGGTGGTGG+TGG	0.532803	2_1:+3208614	None:intergenic
ACAACAACACAAAGCAAACC+AGG	0.533748	2_1:-3208710	Msa0188110:five_prime_UTR
AACCGCAGATCACCTTCAAA+AGG	0.534911	2_1:-3207085	Msa0188110:CDS
TCGCCATGCCGAAAGTTCCT+GGG	0.538733	2_1:-3208470	Msa0188110:CDS
TTGTTTGGGAATGAGGATTG+AGG	0.539065	2_1:+3208549	None:intergenic
GGTTATTTGTAAATATTCAA+GGG	0.542148	2_1:-3207064	Msa0188110:CDS
ATTCGATTGCAGCCATGGAT+GGG	0.543191	2_1:-3207110	Msa0188110:CDS
TAGGGTTCCGAATGTTGCTC+CGG	0.545131	2_1:+3208593	None:intergenic
TCCGGCGATGATTGAGGTGG+TGG	0.546028	2_1:+3208611	None:intergenic
CGGCATGGCGAAGAAGGATG+AGG	0.548351	2_1:+3208482	None:intergenic
GCTTTATACCTCGGAGTGAA+GGG	0.548651	2_1:+3208443	None:intergenic
GATAATGCAGGAGCAAGTGC+AGG	0.552707	2_1:-3207172	Msa0188110:CDS
TCGGAGTGAAGGGTGTTCCC+AGG	0.556542	2_1:+3208453	None:intergenic
ATCTGCGGTTTGCCCATCCA+TGG	0.557110	2_1:+3207098	None:intergenic
ATTGCTCAGAGCTCATGACT+AGG	0.557619	2_1:-3204870	Msa0188110:three_prime_UTR
CTGCACTGCTTGAAACTGCT+GGG	0.562340	2_1:-3206361	Msa0188110:CDS
TTGGGTGGTCTACAGGTGCA+TGG	0.562923	2_1:-3206456	Msa0188110:intron
GTGGTGGAAAGGTGAATTGA+CGG	0.563324	2_1:+3208630	None:intergenic
ACCACCACCTCAATCATCGC+CGG	0.572284	2_1:-3208612	Msa0188110:CDS
CAGGAATCCAACCCAACTGT+TGG	0.577443	2_1:+3207324	None:intergenic
AAATGAGTGTCGACATAATG+GGG	0.577838	2_1:-3208754	None:intergenic
GGAGACACTCAAGATAATGC+AGG	0.579263	2_1:-3207184	Msa0188110:CDS
TCCCTGACTTACTCAGAGGC+CGG	0.580646	2_1:+3207378	None:intergenic
TGGGTGGTCTACAGGTGCAT+GGG	0.587485	2_1:-3206455	Msa0188110:intron
TGTTGCTCCGGCGATGATTG+AGG	0.595491	2_1:+3208605	None:intergenic
GCCTCTGAGTAAGTCAGGGA+AGG	0.597075	2_1:-3207375	Msa0188110:CDS
TACAGGCATTTGTAGCAGCA+GGG	0.598227	2_1:-3205051	Msa0188110:intron
TGATTGAGGTGGTGGTGGAA+AGG	0.598567	2_1:+3208619	None:intergenic
TTGCTCAGAGCTCATGACTA+GGG	0.604327	2_1:-3204869	Msa0188110:three_prime_UTR
GTTCCCAGGAACTTTCGGCA+TGG	0.607984	2_1:+3208467	None:intergenic
AAACCGCATCAGTTTGGTCA+GGG	0.611343	2_1:-3204897	Msa0188110:three_prime_UTR
CTTGCGACAGCGATGCAGCA+CGG	0.627397	2_1:-3207212	Msa0188110:intron
ACCGGCCTCTGAGTAAGTCA+GGG	0.632716	2_1:-3207379	Msa0188110:CDS
TCCTTCCCTGACTTACTCAG+AGG	0.643499	2_1:+3207374	None:intergenic
CAACAACACAAAGCAAACCA+GGG	0.652099	2_1:-3208709	Msa0188110:five_prime_UTR
GGTGCATGGGACACAACAGG+GGG	0.655496	2_1:-3206442	Msa0188110:CDS
AGGTGCATGGGACACAACAG+GGG	0.655761	2_1:-3206443	Msa0188110:CDS
CATGGCGAAGAAGGATGAGG+CGG	0.664817	2_1:+3208485	None:intergenic
ACGCCCTGACCAAACTGATG+CGG	0.673309	2_1:+3204894	None:intergenic
TGGGAACACCCTTCACTCCG+AGG	0.676993	2_1:-3208451	Msa0188110:intron
TGAATTGACGGTGCTGCATG+TGG	0.678087	2_1:+3208642	None:intergenic
ACTAGAAACAAAATGAACCT+CGG	0.684849	2_1:+3207137	None:intergenic
GCAGGAACTACTGGACGCCG+AGG	0.701768	2_1:-3207154	Msa0188110:CDS
TGCTCCGGCGATGATTGAGG+TGG	0.724852	2_1:+3208608	None:intergenic
AATGAGTGTCGACATAATGG+GGG	0.732885	2_1:-3208753	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAAATTAACTTCTAATTAT+AGG	-	chr2_1:3207118-3207137	Msa0188110:CDS	10.0%
!!	CAAAAATAAATTTATAATCT+CGG	+	chr2_1:3207162-3207181	None:intergenic	10.0%
!!	AAGATAAAATAATGTCAAAT+TGG	+	chr2_1:3205664-3205683	None:intergenic	15.0%
!!	AACATAAACATTATTAACAT+GGG	+	chr2_1:3205767-3205786	None:intergenic	15.0%
!!	AAACATAAACATTATTAACA+TGG	+	chr2_1:3205768-3205787	None:intergenic	15.0%
!!!	AAAAGCACAAAAAAAATAAA+TGG	+	chr2_1:3206493-3206512	None:intergenic	15.0%
!!	ATTACATTAATAAGATTTGA+TGG	-	chr2_1:3206770-3206789	Msa0188110:intron	15.0%
!!!	CGAGATTATAAATTTATTTT+TGG	-	chr2_1:3207160-3207179	Msa0188110:CDS	15.0%
!!	AAAGATGATAAAATGATAAT+AGG	-	chr2_1:3207430-3207449	Msa0188110:intron	15.0%
!!!	ATTCTTATTGATTAATGAAT+TGG	-	chr2_1:3207599-3207618	Msa0188110:intron	15.0%
!!	TAAAATATTACTCAATCAAA+AGG	+	chr2_1:3208069-3208088	None:intergenic	15.0%
!!!	TTTGATTGAGTAATATTTTA+AGG	-	chr2_1:3208069-3208088	Msa0188110:intron	15.0%
!!!	TTGATTGAGTAATATTTTAA+GGG	-	chr2_1:3208070-3208089	Msa0188110:intron	15.0%
!!!	AATTATAGTTTTCATAATCA+TGG	+	chr2_1:3208732-3208751	None:intergenic	15.0%
!!	CTTTAATTCCAAAAAATGAA+GGG	+	chr2_1:3204841-3204860	None:intergenic	20.0%
!!	ACTTTAATTCCAAAAAATGA+AGG	+	chr2_1:3204842-3204861	None:intergenic	20.0%
!!	GTTTCAAACTTTATTCAATT+AGG	-	chr2_1:3204860-3204879	Msa0188110:three_prime_UTR	20.0%
!!	AAGAAAAAATAAAGTCAAAC+TGG	+	chr2_1:3205164-3205183	None:intergenic	20.0%
!!	TTGAAATGAAAATGTTTGTT+TGG	-	chr2_1:3205315-3205334	Msa0188110:intron	20.0%
!!	ATGAAAATGTATTGTTTGTT+TGG	-	chr2_1:3205805-3205824	Msa0188110:intron	20.0%
!!!	ATTTACAAATAACCTTTTGA+AGG	+	chr2_1:3206189-3206208	None:intergenic	20.0%
!!	AGGTTATTTGTAAATATTCA+AGG	-	chr2_1:3206194-3206213	Msa0188110:intron	20.0%
!!	GGTTATTTGTAAATATTCAA+GGG	-	chr2_1:3206195-3206214	Msa0188110:intron	20.0%
!!	AATTTAGAAACCAACATAAA+AGG	+	chr2_1:3206265-3206284	None:intergenic	20.0%
!!	TCTTTATTATCTTTGTATGA+AGG	-	chr2_1:3206284-3206303	Msa0188110:intron	20.0%
!!	TCGTAAAGAACTATTTAAAT+CGG	+	chr2_1:3206693-3206712	None:intergenic	20.0%
!!	ATTGTTGGAAAAAGAATAAA+GGG	+	chr2_1:3206717-3206736	None:intergenic	20.0%
!!	TATTGTTGGAAAAAGAATAA+AGG	+	chr2_1:3206718-3206737	None:intergenic	20.0%
!!	AGCAAATAAAAAGATATTGT+TGG	+	chr2_1:3206732-3206751	None:intergenic	20.0%
!!!	ATTTGATGGATATTTGATAT+TGG	-	chr2_1:3206784-3206803	Msa0188110:intron	20.0%
!!!	TTTGATGGATATTTGATATT+GGG	-	chr2_1:3206785-3206804	Msa0188110:intron	20.0%
!!!	TATTTTTGGAATGTTTTTTG+TGG	-	chr2_1:3207174-3207193	Msa0188110:CDS	20.0%
!!	TGATTAATGAATTGGAAAAT+TGG	-	chr2_1:3207607-3207626	Msa0188110:intron	20.0%
!!	TTCCATCATAAATGTTATAA+TGG	-	chr2_1:3207644-3207663	Msa0188110:intron	20.0%
!!	AACCATTATAACATTTATGA+TGG	+	chr2_1:3207649-3207668	None:intergenic	20.0%
!!	CATAAATGTTATAATGGTTT+AGG	-	chr2_1:3207650-3207669	Msa0188110:intron	20.0%
!!!	CACTAATAAGGTTTTATTTT+AGG	+	chr2_1:3207896-3207915	None:intergenic	20.0%
!!	TCAAATCTTATTATCATGAT+AGG	+	chr2_1:3208020-3208039	None:intergenic	20.0%
!!!	TGATTGAGTAATATTTTAAG+GGG	-	chr2_1:3208071-3208090	Msa0188110:intron	20.0%
!	TTTCCACTAAATTCCTTAAA+CGG	+	chr2_1:3204893-3204912	None:intergenic	25.0%
!	AATTGAAACTGCTTATGTTA+TGG	-	chr2_1:3204911-3204930	Msa0188110:three_prime_UTR	25.0%
!!!	TTTTGTGCTATTTTGAATGT+AGG	-	chr2_1:3204971-3204990	Msa0188110:three_prime_UTR	25.0%
!!!	TTTGTGCTATTTTGAATGTA+GGG	-	chr2_1:3204972-3204991	Msa0188110:three_prime_UTR	25.0%
!!	AATAAGTTGAAAACAGCTTA+TGG	+	chr2_1:3205089-3205108	None:intergenic	25.0%
!	ATTAAGCTGTTTATCCAAAT+AGG	-	chr2_1:3205232-3205251	Msa0188110:intron	25.0%
!!!	AAATGTTTGTTTGGTGTAAA+TGG	-	chr2_1:3205324-3205343	Msa0188110:intron	25.0%
!	AATTGAAACACGTTATGTTA+TGG	-	chr2_1:3205414-3205433	Msa0188110:intron	25.0%
!!	ATTAAGTTGTTTATCCAAAC+AGG	-	chr2_1:3205717-3205736	Msa0188110:intron	25.0%
!!	TTAAGTTGTTTATCCAAACA+GGG	-	chr2_1:3205718-3205737	Msa0188110:intron	25.0%
!!!	TATTGTTTGTTTGGTGTAAA+TGG	-	chr2_1:3205814-3205833	Msa0188110:intron	25.0%
!	TTATTATCTTTGTATGAAGG+TGG	-	chr2_1:3206287-3206306	Msa0188110:intron	25.0%
!!!	ACATTGTTTTGTTTTTCCAT+AGG	+	chr2_1:3206358-3206377	None:intergenic	25.0%
!	TATGGAAAAACAAAACAATG+TGG	-	chr2_1:3206357-3206376	Msa0188110:CDS	25.0%
!!!	GATGTTGATTCATTAAGTAT+AGG	+	chr2_1:3206666-3206685	None:intergenic	25.0%
!	CGTAAAGAACTATTTAAATC+GGG	+	chr2_1:3206692-3206711	None:intergenic	25.0%
!!	CTTCATAAAAGTATAAACTG+AGG	+	chr2_1:3207030-3207049	None:intergenic	25.0%
!	TTAGAATGTTCAAGGTTTAT+GGG	+	chr2_1:3207369-3207388	None:intergenic	25.0%
!!	ACATAACTGTTTCAACTTTT+AGG	-	chr2_1:3207523-3207542	Msa0188110:intron	25.0%
!	AAAAATATTAGAAACCGTGA+AGG	+	chr2_1:3207558-3207577	None:intergenic	25.0%
!!!	CGGTTTCTAATATTTTTACA+TGG	-	chr2_1:3207561-3207580	Msa0188110:intron	25.0%
!	GCTTAAAAGGTAAAATATCA+AGG	+	chr2_1:3207874-3207893	None:intergenic	25.0%
!!!	GGTTTTATTTTAGGCTTAAA+AGG	+	chr2_1:3207887-3207906	None:intergenic	25.0%
!	TAAAACCTTATTAGTGTAAC+TGG	-	chr2_1:3207900-3207919	Msa0188110:intron	25.0%
!	ATATTATGTAAGCTTCCAAA+AGG	-	chr2_1:3208133-3208152	Msa0188110:intron	25.0%
!!	AAATCGAATACTTTTCCTTT+TGG	+	chr2_1:3208151-3208170	None:intergenic	25.0%
!!!	TTAGATACACAACTTTTTCT+CGG	+	chr2_1:3208178-3208197	None:intergenic	25.0%
!!	TATTAGCAAGATTTTCAGTA+TGG	+	chr2_1:3208293-3208312	None:intergenic	25.0%
!!!	CACAAGTAACTTTTTTTCTT+TGG	+	chr2_1:3208649-3208668	None:intergenic	25.0%
!!	TATAGTTTTCATAATCATGG+TGG	+	chr2_1:3208729-3208748	None:intergenic	25.0%
!	AAAACTATAATTCCCTGAAT+GGG	-	chr2_1:3208740-3208759	Msa0188110:five_prime_UTR	25.0%
!	ATACCCAATTTCAGATTTTG+TGG	+	chr2_1:3204584-3204603	None:intergenic	30.0%
!!!	GTATAAAGCCCTTCATTTTT+TGG	-	chr2_1:3204830-3204849	Msa0188110:three_prime_UTR	30.0%
	TTCCACTAAATTCCTTAAAC+GGG	+	chr2_1:3204892-3204911	None:intergenic	30.0%
	TTCTGGATAAAACAGATACT+GGG	-	chr2_1:3204941-3204960	Msa0188110:three_prime_UTR	30.0%
!	TTTTGAATGTAGGGTCTATT+TGG	-	chr2_1:3204981-3205000	Msa0188110:three_prime_UTR	30.0%
	ATAACATGAGTGAGTGTTAA+AGG	+	chr2_1:3205266-3205285	None:intergenic	30.0%
	GTGTCAAACTTTATTCAACT+TGG	-	chr2_1:3205362-3205381	Msa0188110:intron	30.0%
	TTATTCAACTTGGAATGTTC+TGG	-	chr2_1:3205372-3205391	Msa0188110:intron	30.0%
	TTCTACGTAAAACAAGTAGT+GGG	-	chr2_1:3205444-3205463	Msa0188110:intron	30.0%
!!	TGACTAATTTTGAGAGCTAA+TGG	-	chr2_1:3205543-3205562	Msa0188110:intron	30.0%
	AATAAGCTCAAAACAGCTTA+TGG	+	chr2_1:3205587-3205606	None:intergenic	30.0%
	TAATACTTTATGACCCTGTT+TGG	+	chr2_1:3205734-3205753	None:intergenic	30.0%
	TTCCTGAACTTATACTATCT+AGG	-	chr2_1:3205952-3205971	Msa0188110:intron	30.0%
	TACCTAGATAGTATAAGTTC+AGG	+	chr2_1:3205957-3205976	None:intergenic	30.0%
	TGAACTTATACTATCTAGGT+AGG	-	chr2_1:3205956-3205975	Msa0188110:intron	30.0%
	GAACTTATACTATCTAGGTA+GGG	-	chr2_1:3205957-3205976	Msa0188110:intron	30.0%
	ACTAGAAACAAAATGAACCT+CGG	+	chr2_1:3206125-3206144	None:intergenic	30.0%
!!	TTTTGTCTAGCCTTTTATGT+TGG	-	chr2_1:3206252-3206271	Msa0188110:intron	30.0%
!	TGTTTTTCCATAGGTTGTTT+GGG	+	chr2_1:3206349-3206368	None:intergenic	30.0%
!!	TTGTTTTTCCATAGGTTGTT+TGG	+	chr2_1:3206350-3206369	None:intergenic	30.0%
	ATAATGCTATATTTGCAGAG+TGG	-	chr2_1:3206402-3206421	Msa0188110:CDS	30.0%
	GGTCAAATATGGAAATTTAG+TGG	-	chr2_1:3206456-3206475	Msa0188110:intron	30.0%
!	CTCTAAAAGTTCAAAACAGA+TGG	+	chr2_1:3206522-3206541	None:intergenic	30.0%
!!	CATCTGTTTTGAACTTTTAG+AGG	-	chr2_1:3206520-3206539	Msa0188110:intron	30.0%
!	TTTTCAACCTTTCAACACTA+TGG	-	chr2_1:3206548-3206567	Msa0188110:intron	30.0%
!!!	GATTCAACCAATTTTGAGTT+GGG	+	chr2_1:3206581-3206600	None:intergenic	30.0%
!!	AGATTCAACCAATTTTGAGT+TGG	+	chr2_1:3206582-3206601	None:intergenic	30.0%
	TTAATGTACACAATTGTGCA+AGG	+	chr2_1:3206953-3206972	None:intergenic	30.0%
!	GTCATTTATTGTGTCTTTTG+TGG	-	chr2_1:3206988-3207007	Msa0188110:intron	30.0%
!!!	GTGTATTCTTGTTCCTTTTT+TGG	-	chr2_1:3207273-3207292	Msa0188110:intron	30.0%
	AGTCAACCAATATTTACCAT+TGG	-	chr2_1:3207300-3207319	Msa0188110:intron	30.0%
	GTCAACCAATATTTACCATT+GGG	-	chr2_1:3207301-3207320	Msa0188110:intron	30.0%
	GTTAGAATGTTCAAGGTTTA+TGG	+	chr2_1:3207370-3207389	None:intergenic	30.0%
	ATTGTGATGTGTGAAATTTG+AGG	+	chr2_1:3207396-3207415	None:intergenic	30.0%
	CAAATTTCACACATCACAAT+TGG	-	chr2_1:3207396-3207415	Msa0188110:intron	30.0%
	AAGTTGAAACAGTTATGTGT+AGG	+	chr2_1:3207521-3207540	None:intergenic	30.0%
	AGTTCCAGTAACTTCAATTA+CGG	-	chr2_1:3207774-3207793	Msa0188110:intron	30.0%
	AAGGTAAAATATCAAGGCTT+GGG	+	chr2_1:3207868-3207887	None:intergenic	30.0%
	AAAGGTAAAATATCAAGGCT+TGG	+	chr2_1:3207869-3207888	None:intergenic	30.0%
	AAGTTCCAGTTACACTAATA+AGG	+	chr2_1:3207908-3207927	None:intergenic	30.0%
	TCTCATTGATATCCTTTCTT+GGG	+	chr2_1:3207962-3207981	None:intergenic	30.0%
	ATCTCATTGATATCCTTTCT+TGG	+	chr2_1:3207963-3207982	None:intergenic	30.0%
	TATCTAACATTGCATCTTAC+AGG	-	chr2_1:3208191-3208210	Msa0188110:intron	30.0%
	AGATGCTCAAAAGATAAAGT+TGG	-	chr2_1:3208312-3208331	Msa0188110:intron	30.0%
!!	GAAGCATCATTGCTTATTTT+AGG	-	chr2_1:3208334-3208353	Msa0188110:intron	30.0%
	CGATCAGATTCTGTTTAATT+TGG	-	chr2_1:3208455-3208474	Msa0188110:CDS	30.0%
	CTTTACATTCTATACTAAGC+AGG	+	chr2_1:3208550-3208569	None:intergenic	30.0%
	GGAAGAAAAGAAAGATGAAA+TGG	+	chr2_1:3208708-3208727	None:intergenic	30.0%
	GAAAACTATAATTCCCTGAA+TGG	-	chr2_1:3208739-3208758	Msa0188110:five_prime_UTR	30.0%
!	AACGCCACAAAATCTGAAAT+TGG	-	chr2_1:3204577-3204596	Msa0188110:three_prime_UTR	35.0%
!	ACGCCACAAAATCTGAAATT+GGG	-	chr2_1:3204578-3204597	Msa0188110:three_prime_UTR	35.0%
!!	TTTAGCTTGGTGTCTTGTTT+GGG	+	chr2_1:3204727-3204746	None:intergenic	35.0%
!!!	TTTTAGCTTGGTGTCTTGTT+TGG	+	chr2_1:3204728-3204747	None:intergenic	35.0%
!	AAATGAAGGGCTTTATACCT+CGG	+	chr2_1:3204828-3204847	None:intergenic	35.0%
	TTCCCGTTTAAGGAATTTAG+TGG	-	chr2_1:3204887-3204906	Msa0188110:three_prime_UTR	35.0%
!!	TATGTTATGGCTTCTAGTTC+TGG	-	chr2_1:3204924-3204943	Msa0188110:three_prime_UTR	35.0%
!	GTTCTGGATAAAACAGATAC+TGG	-	chr2_1:3204940-3204959	Msa0188110:three_prime_UTR	35.0%
	AAGCACTTGTATGAGTGTTT+AGG	-	chr2_1:3205034-3205053	Msa0188110:CDS	35.0%
	AGCACTTGTATGAGTGTTTA+GGG	-	chr2_1:3205035-3205054	Msa0188110:CDS	35.0%
	AAGCTAACACAGAGAACTTA+TGG	+	chr2_1:3205113-3205132	None:intergenic	35.0%
	ATGAGTGAGTGTTAAAGGTT+GGG	+	chr2_1:3205261-3205280	None:intergenic	35.0%
!!	TGTTTGTTTGGTGTAAATGG+AGG	-	chr2_1:3205327-3205346	Msa0188110:intron	35.0%
!!	ACTTGGAATGTTCTGGTTTA+AGG	-	chr2_1:3205379-3205398	Msa0188110:intron	35.0%
	GTTCTACGTAAAACAAGTAG+TGG	-	chr2_1:3205443-3205462	Msa0188110:intron	35.0%
!!	CTTTGTGCTATTTCAAGTGT+AGG	-	chr2_1:3205474-3205493	Msa0188110:intron	35.0%
!!	TTTCAAGTGTAGGACTAGTT+TGG	-	chr2_1:3205484-3205503	Msa0188110:intron	35.0%
!!	TGTTTGTTTGGTGTAAATGG+AGG	-	chr2_1:3205327-3205346	Msa0188110:intron	35.0%
	TTATCTTTGTATGAAGGTGG+AGG	-	chr2_1:3206290-3206309	Msa0188110:intron	35.0%
	ACTGAGAAATCAGATGGTAA+AGG	-	chr2_1:3206435-3206454	Msa0188110:CDS	35.0%
	CAGATGGTAAAGGTCAAATA+TGG	-	chr2_1:3206445-3206464	Msa0188110:intron	35.0%
!	TTTCAGTCCATAGTGTTGAA+AGG	+	chr2_1:3206558-3206577	None:intergenic	35.0%
	ACTGAAACCCAACTCAAAAT+TGG	-	chr2_1:3206571-3206590	Msa0188110:intron	35.0%
	GAAGAACATCCAATGTTTGT+AGG	-	chr2_1:3206607-3206626	Msa0188110:intron	35.0%
	AAGAACTATTTAAATCGGGC+TGG	+	chr2_1:3206688-3206707	None:intergenic	35.0%
	GATGGATATTTGATATTGGG+TGG	-	chr2_1:3206788-3206807	Msa0188110:intron	35.0%
	AAGATCGCACAGAAATTGTA+TGG	-	chr2_1:3207066-3207085	Msa0188110:CDS	35.0%
	AGATCGCACAGAAATTGTAT+GGG	-	chr2_1:3207067-3207086	Msa0188110:CDS	35.0%
	TTTGTGGAACATGTGTTTGA+AGG	-	chr2_1:3207190-3207209	Msa0188110:CDS	35.0%
	TTGTGGAACATGTGTTTGAA+GGG	-	chr2_1:3207191-3207210	Msa0188110:CDS	35.0%
	TTATCTCCCTACAAAACTGT+AGG	-	chr2_1:3207245-3207264	Msa0188110:intron	35.0%
	GACATCCCAATGGTAAATAT+TGG	+	chr2_1:3207309-3207328	None:intergenic	35.0%
	GAGGTAAGTTAGAATGTTCA+AGG	+	chr2_1:3207377-3207396	None:intergenic	35.0%
	AGTTATGTGTAGGTCATGAA+TGG	+	chr2_1:3207511-3207530	None:intergenic	35.0%
	CATGTGGGTACTAACAAATA+GGG	+	chr2_1:3207707-3207726	None:intergenic	35.0%
	ACATGTGGGTACTAACAAAT+AGG	+	chr2_1:3207708-3207727	None:intergenic	35.0%
	CTTGGGGACTAATCATATAA+GGG	+	chr2_1:3207851-3207870	None:intergenic	35.0%
	AGGTAAAATATCAAGGCTTG+GGG	+	chr2_1:3207867-3207886	None:intergenic	35.0%
	CTCATTGATATCCTTTCTTG+GGG	+	chr2_1:3207961-3207980	None:intergenic	35.0%
	AAAGCATACTAGATGGAAGA+TGG	-	chr2_1:3208413-3208432	Msa0188110:intron	35.0%
!!	TCTGTTTAATTTGGCTGCAA+TGG	-	chr2_1:3208464-3208483	Msa0188110:CDS	35.0%
!!	CTGTTTAATTTGGCTGCAAT+GGG	-	chr2_1:3208465-3208484	Msa0188110:CDS	35.0%
	CATTGCTGAGAGTAAATTGT+TGG	-	chr2_1:3208515-3208534	Msa0188110:CDS	35.0%
!	TATAATTCCCTGAATGGGTT+GGG	-	chr2_1:3208745-3208764	Msa0188110:five_prime_UTR	35.0%
	ACAACAACACAAAGCAAACC+AGG	-	chr2_1:3204549-3204568	Msa0188110:three_prime_UTR	40.0%
	CAACAACACAAAGCAAACCA+GGG	-	chr2_1:3204550-3204569	Msa0188110:three_prime_UTR	40.0%
!	ATTTTGTGGCGTTAAATCCC+TGG	+	chr2_1:3204570-3204589	None:intergenic	40.0%
!	TGGCGCGTAATCTGAAATTA+GGG	+	chr2_1:3204687-3204706	None:intergenic	40.0%
!	TTGGCGCGTAATCTGAAATT+AGG	+	chr2_1:3204688-3204707	None:intergenic	40.0%
	TTGTTTGGGAATGAGGATTG+AGG	+	chr2_1:3204713-3204732	None:intergenic	40.0%
!!	TGAGAAAGGCTCTTTTAGCT+TGG	+	chr2_1:3204740-3204759	None:intergenic	40.0%
	ATTAGGAACGTTCCCGTTTA+AGG	-	chr2_1:3204877-3204896	Msa0188110:three_prime_UTR	40.0%
	GCACTTGTATGAGTGTTTAG+GGG	-	chr2_1:3205036-3205055	Msa0188110:CDS	40.0%
	TAAAGGTTGGGACTCCTATT+TGG	+	chr2_1:3205249-3205268	None:intergenic	40.0%
	CATGAGTGAGTGTTAAAGGT+TGG	+	chr2_1:3205262-3205281	None:intergenic	40.0%
!	TATCTAGGTAGGGAGTTTTG+TGG	-	chr2_1:3205967-3205986	Msa0188110:intron	40.0%
	TTTCTATTCGATTGCAGCCA+TGG	-	chr2_1:3206144-3206163	Msa0188110:intron	40.0%
!!	AACCTTTTGAAGGTGATCTG+CGG	+	chr2_1:3206179-3206198	None:intergenic	40.0%
	TATGAAGGTGGAGGTTGAAT+GGG	-	chr2_1:3206299-3206318	Msa0188110:intron	40.0%
!	AGGTTGTTTGGGTTCAAACA+AGG	+	chr2_1:3206338-3206357	None:intergenic	40.0%
	GTTTGAACCCAAACAACCTA+TGG	-	chr2_1:3206339-3206358	Msa0188110:intron	40.0%
	GCTACAACTGAGAAATCAGA+TGG	-	chr2_1:3206429-3206448	Msa0188110:CDS	40.0%
	GAACATCCAATGTTTGTAGG+TGG	-	chr2_1:3206610-3206629	Msa0188110:intron	40.0%
	TAGTGACCACCTACAAACAT+TGG	+	chr2_1:3206619-3206638	None:intergenic	40.0%
	TTTGATATTGGGTGGTCTAC+AGG	-	chr2_1:3206796-3206815	Msa0188110:intron	40.0%
!	AGGCAGCTTCAATGTTTTGT+GGG	-	chr2_1:3206847-3206866	Msa0188110:intron	40.0%
	TTGAAACTGCTGGGAAATCT+TGG	-	chr2_1:3206907-3206926	Msa0188110:intron	40.0%
!!	CACACTCCTACAGTTTTGTA+GGG	+	chr2_1:3207254-3207273	None:intergenic	40.0%
!!	ACACACTCCTACAGTTTTGT+AGG	+	chr2_1:3207255-3207274	None:intergenic	40.0%
!	TGGTTGACTCTAGCCAAAAA+AGG	+	chr2_1:3207289-3207308	None:intergenic	40.0%
	ATGCTAGTAAGACATCCCAA+TGG	+	chr2_1:3207319-3207338	None:intergenic	40.0%
	TGTCTTACTAGCATGATGGT+AGG	-	chr2_1:3207325-3207344	Msa0188110:CDS	40.0%
	TTAGGAGAGTTAGTCCTTCA+CGG	-	chr2_1:3207541-3207560	Msa0188110:intron	40.0%
	ACATGAAGCTAGAGGTCATA+AGG	+	chr2_1:3207681-3207700	None:intergenic	40.0%
	CAGTAACTTCAATTACGGCA+GGG	-	chr2_1:3207779-3207798	Msa0188110:intron	40.0%
	GCTTGGGGACTAATCATATA+AGG	+	chr2_1:3207852-3207871	None:intergenic	40.0%
	GTGTCAAGCATAATAGCTAC+TGG	+	chr2_1:3207935-3207954	None:intergenic	40.0%
	TTGATATCCTTTCTTGGGGT+AGG	+	chr2_1:3207957-3207976	None:intergenic	40.0%
	CAATCAAAAGGTAACCGTAG+TGG	+	chr2_1:3208057-3208076	None:intergenic	40.0%
!	ATCTTGACTAGCTAGCTACT+TGG	+	chr2_1:3208267-3208286	None:intergenic	40.0%
!	ACTAGGGAAAGCATACTAGA+TGG	-	chr2_1:3208406-3208425	Msa0188110:intron	40.0%
	CTATAATTCCCTGAATGGGT+TGG	-	chr2_1:3208744-3208763	Msa0188110:five_prime_UTR	40.0%
!	GTGGTGGAAAGGTGAATTGA+CGG	+	chr2_1:3204632-3204651	None:intergenic	45.0%
	GGGAATGAGGATTGAGGATT+TGG	+	chr2_1:3204707-3204726	None:intergenic	45.0%
!!	TGGTGTCTTGTTTGGGAATG+AGG	+	chr2_1:3204720-3204739	None:intergenic	45.0%
	CGAGAGATGGAATCTGAGAA+AGG	+	chr2_1:3204754-3204773	None:intergenic	45.0%
	GCTTTATACCTCGGAGTGAA+GGG	+	chr2_1:3204819-3204838	None:intergenic	45.0%
	TTCCCTCAAACTCCAACAGT+TGG	-	chr2_1:3205923-3205942	Msa0188110:intron	45.0%
	TCCCTCAAACTCCAACAGTT+GGG	-	chr2_1:3205924-3205943	Msa0188110:intron	45.0%
	ACCCAACTGTTGGAGTTTGA+GGG	+	chr2_1:3205928-3205947	None:intergenic	45.0%
	AACCCAACTGTTGGAGTTTG+AGG	+	chr2_1:3205929-3205948	None:intergenic	45.0%
	TCAAACTCCAACAGTTGGGT+TGG	-	chr2_1:3205928-3205947	Msa0188110:intron	45.0%
!!	GAGTTTGCTGTCTGTCTTTG+AGG	-	chr2_1:3206004-3206023	Msa0188110:intron	45.0%
	GGAGACACTCAAGATAATGC+AGG	-	chr2_1:3206075-3206094	Msa0188110:intron	45.0%
	TATTCGATTGCAGCCATGGA+TGG	-	chr2_1:3206148-3206167	Msa0188110:intron	45.0%
	ATTCGATTGCAGCCATGGAT+GGG	-	chr2_1:3206149-3206168	Msa0188110:intron	45.0%
	AACCGCAGATCACCTTCAAA+AGG	-	chr2_1:3206174-3206193	Msa0188110:intron	45.0%
	GTATGAAGGTGGAGGTTGAA+TGG	-	chr2_1:3206298-3206317	Msa0188110:intron	45.0%
!	GAGGCAGCTTCAATGTTTTG+TGG	-	chr2_1:3206846-3206865	Msa0188110:intron	45.0%
!	GGCAGCTTCAATGTTTTGTG+GGG	-	chr2_1:3206848-3206867	Msa0188110:intron	45.0%
	TGCTGGGAAATCTTGGAATG+AGG	-	chr2_1:3206914-3206933	Msa0188110:intron	45.0%
	GGGATGTCTTACTAGCATGA+TGG	-	chr2_1:3207321-3207340	Msa0188110:CDS	45.0%
!	TAGGGAGTACATGAAGCTAG+AGG	+	chr2_1:3207689-3207708	None:intergenic	45.0%
	GAATGTTGTGTGCGACATGT+GGG	+	chr2_1:3207722-3207741	None:intergenic	45.0%
	TGAATGTTGTGTGCGACATG+TGG	+	chr2_1:3207723-3207742	None:intergenic	45.0%
	CCTGCCGTAATTGAAGTTAC+TGG	+	chr2_1:3207781-3207800	None:intergenic	45.0%
	CCAGTAACTTCAATTACGGC+AGG	-	chr2_1:3207778-3207797	Msa0188110:intron	45.0%
	AACATAGTCGCACACCACTA+CGG	-	chr2_1:3208040-3208059	Msa0188110:intron	45.0%
	TTACAGGCATTTGTAGCAGC+AGG	-	chr2_1:3208207-3208226	Msa0188110:intron	45.0%
	TACAGGCATTTGTAGCAGCA+GGG	-	chr2_1:3208208-3208227	Msa0188110:intron	45.0%
	GAATCCAAACCGCATCAGTT+TGG	-	chr2_1:3208356-3208375	Msa0188110:intron	45.0%
	AAACCGCATCAGTTTGGTCA+GGG	-	chr2_1:3208362-3208381	Msa0188110:intron	45.0%
	ATTGCTCAGAGCTCATGACT+AGG	-	chr2_1:3208389-3208408	Msa0188110:intron	45.0%
	TTGCTCAGAGCTCATGACTA+GGG	-	chr2_1:3208390-3208409	Msa0188110:intron	45.0%
!	TCGACATAATGGGGGCACAA+TGG	-	chr2_1:3204514-3204533	Msa0188110:three_prime_UTR	50.0%
!!	TGAATTGACGGTGCTGCATG+TGG	+	chr2_1:3204620-3204639	None:intergenic	50.0%
!!	TGATTGAGGTGGTGGTGGAA+AGG	+	chr2_1:3204643-3204662	None:intergenic	50.0%
	ATCATCGCCGGAGCAACATT+CGG	-	chr2_1:3204659-3204678	Msa0188110:three_prime_UTR	50.0%
	TAGGGTTCCGAATGTTGCTC+CGG	+	chr2_1:3204669-3204688	None:intergenic	50.0%
	AACTTTCGGCATGGCGAAGA+AGG	+	chr2_1:3204786-3204805	None:intergenic	50.0%
!	AGGGTGTTCCCAGGAACTTT+CGG	+	chr2_1:3204800-3204819	None:intergenic	50.0%
!	GGCTTTATACCTCGGAGTGA+AGG	+	chr2_1:3204820-3204839	None:intergenic	50.0%
	TGCGTTGTGCAGATCATGAC+CGG	-	chr2_1:3205862-3205881	Msa0188110:intron	50.0%
	TCCTTCCCTGACTTACTCAG+AGG	+	chr2_1:3205888-3205907	None:intergenic	50.0%
	CAGGAATCCAACCCAACTGT+TGG	+	chr2_1:3205938-3205957	None:intergenic	50.0%
	GATAATGCAGGAGCAAGTGC+AGG	-	chr2_1:3206087-3206106	Msa0188110:intron	50.0%
	CTGCACTGCTTGAAACTGCT+GGG	-	chr2_1:3206898-3206917	Msa0188110:intron	50.0%
!	CAGGGAATGTCTTTTGCTCG+TGG	-	chr2_1:3207797-3207816	Msa0188110:intron	50.0%
	CTTGACACCTACCCCAAGAA+AGG	-	chr2_1:3207947-3207966	Msa0188110:intron	50.0%
	GCATTTGTAGCAGCAGGGTT+TGG	-	chr2_1:3208213-3208232	Msa0188110:intron	50.0%
	TTTGTAGCAGCAGGGTTTGG+TGG	-	chr2_1:3208216-3208235	Msa0188110:intron	50.0%
!	TTGTAGCAGCAGGGTTTGGT+GGG	-	chr2_1:3208217-3208236	Msa0188110:intron	50.0%
!	TTTGGTGGGTGGAAGCTTCA+GGG	-	chr2_1:3208231-3208250	Msa0188110:intron	50.0%
	CTGACCAAACTGATGCGGTT+TGG	+	chr2_1:3208363-3208382	None:intergenic	50.0%
	CAAACCGCATCAGTTTGGTC+AGG	-	chr2_1:3208361-3208380	Msa0188110:intron	50.0%
	ACCACCACCTCAATCATCGC+CGG	-	chr2_1:3204647-3204666	Msa0188110:three_prime_UTR	55.0%
	TGTTGCTCCGGCGATGATTG+AGG	+	chr2_1:3204657-3204676	None:intergenic	55.0%
	CATGGCGAAGAAGGATGAGG+CGG	+	chr2_1:3204777-3204796	None:intergenic	55.0%
	TTCGCCATGCCGAAAGTTCC+TGG	-	chr2_1:3204788-3204807	Msa0188110:three_prime_UTR	55.0%
	TCGCCATGCCGAAAGTTCCT+GGG	-	chr2_1:3204789-3204808	Msa0188110:three_prime_UTR	55.0%
	GTTCCCAGGAACTTTCGGCA+TGG	+	chr2_1:3204795-3204814	None:intergenic	55.0%
	ACCGGCCTCTGAGTAAGTCA+GGG	-	chr2_1:3205880-3205899	Msa0188110:intron	55.0%
	TCCCTGACTTACTCAGAGGC+CGG	+	chr2_1:3205884-3205903	None:intergenic	55.0%
	GCCTCTGAGTAAGTCAGGGA+AGG	-	chr2_1:3205884-3205903	Msa0188110:intron	55.0%
	GGAGCAAGTGCAGGAACTAC+TGG	-	chr2_1:3206096-3206115	Msa0188110:intron	55.0%
	ATCTGCGGTTTGCCCATCCA+TGG	+	chr2_1:3206164-3206183	None:intergenic	55.0%
	TTGGGTGGTCTACAGGTGCA+TGG	-	chr2_1:3206803-3206822	Msa0188110:intron	55.0%
	TGGGTGGTCTACAGGTGCAT+GGG	-	chr2_1:3206804-3206823	Msa0188110:intron	55.0%
	ACAGGTGCATGGGACACAAC+AGG	-	chr2_1:3206814-3206833	Msa0188110:intron	55.0%
	CAGGTGCATGGGACACAACA+GGG	-	chr2_1:3206815-3206834	Msa0188110:intron	55.0%
	AGGTGCATGGGACACAACAG+GGG	-	chr2_1:3206816-3206835	Msa0188110:intron	55.0%
	GCTGCACTGCTTGAAACTGC+TGG	-	chr2_1:3206897-3206916	Msa0188110:intron	55.0%
!	GTTTGGTGGGTGGAAGCTTC+AGG	-	chr2_1:3208230-3208249	Msa0188110:intron	55.0%
	ACGCCCTGACCAAACTGATG+CGG	+	chr2_1:3208368-3208387	None:intergenic	55.0%
!	GGCGATGATTGAGGTGGTGG+TGG	+	chr2_1:3204648-3204667	None:intergenic	60.0%
	TCCGGCGATGATTGAGGTGG+TGG	+	chr2_1:3204651-3204670	None:intergenic	60.0%
	TGCTCCGGCGATGATTGAGG+TGG	+	chr2_1:3204654-3204673	None:intergenic	60.0%
	AAGGATGAGGCGGCGAGAGA+TGG	+	chr2_1:3204767-3204786	None:intergenic	60.0%
	CGGCATGGCGAAGAAGGATG+AGG	+	chr2_1:3204780-3204799	None:intergenic	60.0%
!!	TCGGAGTGAAGGGTGTTCCC+AGG	+	chr2_1:3204809-3204828	None:intergenic	60.0%
	TGGGAACACCCTTCACTCCG+AGG	-	chr2_1:3204808-3204827	Msa0188110:three_prime_UTR	60.0%
	GACCGGCCTCTGAGTAAGTC+AGG	-	chr2_1:3205879-3205898	Msa0188110:intron	60.0%
	CTTGCGACAGCGATGCAGCA+CGG	-	chr2_1:3206047-3206066	Msa0188110:intron	60.0%
!	CAGCGATGCAGCACGGACTA+AGG	-	chr2_1:3206054-3206073	Msa0188110:intron	60.0%
	GGTGCATGGGACACAACAGG+GGG	-	chr2_1:3206817-3206836	Msa0188110:intron	60.0%
	CATGGGACACAACAGGGGGT+GGG	-	chr2_1:3206821-3206840	Msa0188110:intron	60.0%
	ACACAACAGGGGGTGGGAAG+AGG	-	chr2_1:3206827-3206846	Msa0188110:intron	60.0%
!!	TAGCAGCAGGGTTTGGTGGG+TGG	-	chr2_1:3208220-3208239	Msa0188110:intron	60.0%
	GCAGGAACTACTGGACGCCG+AGG	-	chr2_1:3206105-3206124	Msa0188110:intron	65.0%
	GCATGGGACACAACAGGGGG+TGG	-	chr2_1:3206820-3206839	Msa0188110:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	3204513	3208768	3204513	ID=Msa0188110;Name=Msa0188110
chr2_1	mRNA	3204513	3208768	3204513	ID=Msa0188110-mRNA-1;Parent=Msa0188110;Name=Msa0188110-mRNA-1;_AED=0.10;_eAED=0.10;_QI=94\|0.6\|0.83\|1\|0.6\|0.66\|6\|493\|229
chr2_1	exon	3204513	3205068	3204513	ID=Msa0188110-mRNA-1:exon:1387;Parent=Msa0188110-mRNA-1
chr2_1	exon	3206346	3206463	3206346	ID=Msa0188110-mRNA-1:exon:1386;Parent=Msa0188110-mRNA-1
chr2_1	exon	3206970	3206975	3206970	ID=Msa0188110-mRNA-1:exon:1385;Parent=Msa0188110-mRNA-1
chr2_1	exon	3207045	3207224	3207045	ID=Msa0188110-mRNA-1:exon:1384;Parent=Msa0188110-mRNA-1
chr2_1	exon	3207308	3207407	3207308	ID=Msa0188110-mRNA-1:exon:1383;Parent=Msa0188110-mRNA-1
chr2_1	exon	3208452	3208768	3208452	ID=Msa0188110-mRNA-1:exon:1382;Parent=Msa0188110-mRNA-1
chr2_1	five_prime_UTR	3208675	3208768	3208675	ID=Msa0188110-mRNA-1:five_prime_utr;Parent=Msa0188110-mRNA-1
chr2_1	CDS	3208452	3208674	3208452	ID=Msa0188110-mRNA-1:cds;Parent=Msa0188110-mRNA-1
chr2_1	CDS	3207308	3207407	3207308	ID=Msa0188110-mRNA-1:cds;Parent=Msa0188110-mRNA-1
chr2_1	CDS	3207045	3207224	3207045	ID=Msa0188110-mRNA-1:cds;Parent=Msa0188110-mRNA-1
chr2_1	CDS	3206970	3206975	3206970	ID=Msa0188110-mRNA-1:cds;Parent=Msa0188110-mRNA-1
chr2_1	CDS	3206346	3206463	3206346	ID=Msa0188110-mRNA-1:cds;Parent=Msa0188110-mRNA-1
chr2_1	CDS	3205006	3205068	3205006	ID=Msa0188110-mRNA-1:cds;Parent=Msa0188110-mRNA-1
chr2_1	three_prime_UTR	3204513	3205005	3204513	ID=Msa0188110-mRNA-1:three_prime_utr;Parent=Msa0188110-mRNA-1

Gene Sequence

>Msa0188110

ATGCATTCTACCACATGCAGCACCGTCAATTCACCTTTCCACCACCACCTCAATCATCGCCGGAGCAACATTCGGAACCCTAATTTCAGATTACGCGCCAAATCCTCAATCCTCATTCCCAAACAAGACACCAAGCTAAAAGAGCCTTTCTCAGATTCCATCTCTCGCCGCCTCATCCTTCTTCGCCATGCCGAAAGTTCCTGGGAACACCCTTCACTCCGAGATCATGACCGGCCTCTGAGTAAGTCAGGGAAGGAAGATGCTGTGAAAGTTTCCCTCAAACTCCAACAGTTGGGTTGGATTCCTGAACTTATACTATCTAGCGATGCAGCACGGACTAAGGAGACACTCAAGATAATGCAGGAGCAAGTGCAGGAACTACTGGACGCCGAGGTTCATTTTGTTTCTAGTTTCTATTCGATTGCAGCCATGGATGGGCAAACCGCAGATCACCTTCAAAAGGTTATTTGTAAATATTCAAGGGATGAGATATTAACTGTCATGTGGAGGTGCATGGGACACAACAGGGGGTGGGAAGAGGCAGCTTCAATGTTTTGTGGGGCATCTGTAGAATTAAAGACATGCAATGCTGCACTGCTTGAAACTGCTGGGAAATCTTGGAATGAGGCATTTGTAGCAGCAGGGTTTGGTGGGTGGAAGCTTCAGGGCATATTAAAACCAAGTAGCTAG

Protein sequence

>Msa0188110

MHSTTCSTVNSPFHHHLNHRRSNIRNPNFRLRAKSSILIPKQDTKLKEPFSDSISRRLILLRHAESSWEHPSLRDHDRPLSKSGKEDAVKVSLKLQQLGWIPELILSSDAARTKETLKIMQEQVQELLDAEVHFVSSFYSIAAMDGQTADHLQKVICKYSRDEILTVMWRCMGHNRGWEEAASMFCGASVELKTCNAALLETAGKSWNEAFVAAGFGGWKLQGILKPSS*