AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1393720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393720 MtrunA17_Chr3g0128491 94.647 467 20 1 1 462 1 467 0.0 863
Msa1393720 MtrunA17_Chr2g0332311 29.341 334 176 10 127 456 376 653 1.92e-25 109
Msa1393720 MtrunA17_Chr4g0003831 27.003 337 186 11 125 456 370 651 5.71e-25 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393720 AT3G10840.1 56.295 421 158 8 49 458 52 457 1.15e-154 447
Msa1393720 AT3G10840.3 57.248 407 148 8 63 458 5 396 7.34e-154 443
Msa1393720 AT3G10840.2 59.067 386 136 7 84 458 8 382 1.83e-153 441
Msa1393720 AT3G10840.4 59.067 386 136 7 84 458 8 382 1.83e-153 441
Msa1393720 AT1G15490.4 27.034 381 213 12 77 449 164 487 3.52e-27 114
Msa1393720 AT1G15490.2 27.110 391 217 13 77 459 245 575 5.06e-27 114
Msa1393720 AT1G15490.1 27.110 391 217 13 77 459 316 646 1.07e-26 114
Msa1393720 AT1G80280.1 27.895 380 202 13 79 449 326 642 2.42e-26 112
Msa1393720 AT1G52750.1 28.440 327 174 9 127 449 356 626 3.59e-23 103
Msa1393720 AT1G15490.3 31.447 159 97 5 77 230 316 467 1.80e-12 69.7

Find 129 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAATTATGTATTCAATTTC+TGG 0.070082 tig0021758:-14798 Msa1393720:three_prime_UTR
AAAATGACTGCAAATGATTC+TGG 0.236466 tig0021758:-14654 Msa1393720:three_prime_UTR
CTCTCTGAATTGTGGACAAT+TGG 0.271000 tig0021758:-17865 Msa1393720:CDS
ATTCACTGCTGCAATGCTTC+TGG 0.272675 tig0021758:-15562 Msa1393720:CDS
CCCCACTAAAATTGCCTTCT+CGG 0.277300 tig0021758:+17118 None:intergenic
TCACGATATTTGCTTCTTCA+TGG 0.284256 tig0021758:-17788 Msa1393720:CDS
ATCCAATTGTTGGACTTTAC+AGG 0.285682 tig0021758:-16317 Msa1393720:CDS
TAGTGTCTTCCTGTATTATA+TGG 0.287923 tig0021758:+15105 None:intergenic
TTGTTATGAGAAGCATAATT+TGG 0.289447 tig0021758:+17762 None:intergenic
AACTGTTGTCTTCTTTCCTT+TGG 0.297738 tig0021758:+16352 None:intergenic
TCTTCTTCCCTCGGTGGTTC+AGG 0.303294 tig0021758:-17720 Msa1393720:intron
ACATGCTCAGAGACCTGTTT+TGG 0.311785 tig0021758:+15758 None:intergenic
CAATTGTTGAAAACTTTCTA+AGG 0.319019 tig0021758:-15222 Msa1393720:CDS
ATGTATTCAATTTCTGGAAA+TGG 0.328235 tig0021758:-14792 Msa1393720:three_prime_UTR
CAGCGTTTGGATTGACATCT+AGG 0.331482 tig0021758:-17241 Msa1393720:CDS
AGCATGACGAGACCAGAAAA+TGG 0.356456 tig0021758:-15016 Msa1393720:three_prime_UTR
TCTATCCTGCGTTCTTCCCC+TGG 0.359604 tig0021758:-16195 Msa1393720:CDS
CCCACAGCCTATGCTATAAA+TGG 0.367868 tig0021758:+14863 None:intergenic
ATTAGAGCAGCAACCCGTTC+AGG 0.371410 tig0021758:+16444 None:intergenic
GCCATGGAGTATGCATTTAG+AGG 0.382748 tig0021758:+17185 None:intergenic
TTGTACAAAATTCATGTTAC+GGG 0.385371 tig0021758:+15163 None:intergenic
ATGATTCTGTTGCATGGATT+CGG 0.385624 tig0021758:-17344 Msa1393720:CDS
AGACCAGAAAATGGAAAGAT+TGG 0.389292 tig0021758:-15007 Msa1393720:three_prime_UTR
CAGCTTGTTGGTTTGTGCTT+GGG 0.390201 tig0021758:+17376 None:intergenic
TTCAGAGCAGTTGTTGGATG+TGG 0.391657 tig0021758:-17549 Msa1393720:intron
GCCATTGAGGGTTAAGGATT+GGG 0.400126 tig0021758:-15601 Msa1393720:CDS
AAATTCATGTTACGGGTTGT+AGG 0.405204 tig0021758:+15170 None:intergenic
GTCACACGAAATCCAATTGT+TGG 0.405598 tig0021758:-16327 Msa1393720:CDS
GTAAACTTAACTTCTGGAAA+TGG 0.406321 tig0021758:-17218 Msa1393720:CDS
GTGATCGACTAGTTCCTTCA+TGG 0.407021 tig0021758:-15345 Msa1393720:CDS
GCTTCTGCTTCTTCTTCCCT+CGG 0.411248 tig0021758:-17729 Msa1393720:CDS
AAATGGTCTTACAGATCATC+CGG 0.412407 tig0021758:+14880 None:intergenic
TGCAATGTACATGATAATCT+TGG 0.413417 tig0021758:+16287 None:intergenic
AATGCAGCCATTGAGGGTTA+AGG 0.418759 tig0021758:-15607 Msa1393720:intron
TCTGGATCAGCTAATAGCTT+TGG 0.418943 tig0021758:+17470 None:intergenic
AGAAAATTGGGTGTAGTAAG+TGG 0.424399 tig0021758:+16396 None:intergenic
AGAATGGCAATTGATAAGTT+TGG 0.426361 tig0021758:-15812 Msa1393720:CDS
ATATCTTCAAGGACTATAGA+AGG 0.438079 tig0021758:+14922 None:intergenic
GCATTGAATGCAGCCATTGA+GGG 0.446087 tig0021758:-15613 Msa1393720:intron
AATCCATGCAACAGAATCAT+AGG 0.447499 tig0021758:+17347 None:intergenic
GTCTCTGAGCATGTCCTTAG+TGG 0.450535 tig0021758:-15749 Msa1393720:CDS
TCTCTGAATTGTGGACAATT+GGG 0.453789 tig0021758:-17864 Msa1393720:CDS
ATATTTCGAAGCTCCTGAAC+GGG 0.453927 tig0021758:-16457 Msa1393720:CDS
TCTAGGGTAAACTTAACTTC+TGG 0.455409 tig0021758:-17224 Msa1393720:CDS
AACTCAATAAATTGAATGAC+TGG 0.455682 tig0021758:+14742 None:intergenic
TTCTGGAAATGGATAGCTGC+AGG 0.456060 tig0021758:-14781 Msa1393720:three_prime_UTR
TTGTGCTTGGGAATGAGATT+CGG 0.459568 tig0021758:+17388 None:intergenic
CATAGGCAAACACAGCTTGT+TGG 0.461823 tig0021758:+17364 None:intergenic
TGCTGCAATGCTTCTGGACG+AGG 0.462142 tig0021758:-15556 Msa1393720:CDS
AGCCATTGAGGGTTAAGGAT+TGG 0.464429 tig0021758:-15602 Msa1393720:CDS
CTTGTACAAAATTCATGTTA+CGG 0.468317 tig0021758:+15162 None:intergenic
GAACATGTAGCCTCGGCTAA+AGG 0.470554 tig0021758:+17287 None:intergenic
ATGTGGGATCAAAGAAGAAA+AGG 0.473757 tig0021758:-17532 Msa1393720:CDS
GTCTTCCTGTATTATATGGA+TGG 0.473909 tig0021758:+15109 None:intergenic
AGGTACTGAATGAGAGTTAG+TGG 0.477591 tig0021758:-17947 Msa1393720:five_prime_UTR
AAGAAAAGGAAAGAGATAGC+TGG 0.481075 tig0021758:-17518 Msa1393720:CDS
GAAAATGGAAAGATTGGAAG+TGG 0.481190 tig0021758:-15001 Msa1393720:three_prime_UTR
AAAATCCATCCATATAATAC+AGG 0.481485 tig0021758:-15114 Msa1393720:three_prime_UTR
AGAGATAGCTGGAATAGATC+AGG 0.481817 tig0021758:-17507 Msa1393720:CDS
TCCCAATCCTTAACCCTCAA+TGG 0.484351 tig0021758:+15600 None:intergenic
TTCATGGTATGATCCAAAAC+AGG 0.485270 tig0021758:-15771 Msa1393720:CDS
CATGAATGTGTAGAGCTAGT+AGG 0.486151 tig0021758:+17831 None:intergenic
TAAGGATTGGGATAGGGCAC+TGG 0.491068 tig0021758:-15589 Msa1393720:CDS
AGAAGAAGCAGAAGCAATGA+AGG 0.493901 tig0021758:+17737 None:intergenic
TTGTTGTTCAGAGCAGTTGT+TGG 0.494913 tig0021758:-17555 Msa1393720:intron
ACATAGAGAAGATATCTTCA+AGG 0.496328 tig0021758:+14911 None:intergenic
AACCTGTAAAGTCCAACAAT+TGG 0.497943 tig0021758:+16315 None:intergenic
TTGCCTATGATTCTGTTGCA+TGG 0.498619 tig0021758:-17350 Msa1393720:CDS
GTTGCATGGATTCGGAGCCT+CGG 0.501535 tig0021758:-17336 Msa1393720:CDS
CACTAGTGCAATTGAACAGA+TGG 0.504881 tig0021758:-15087 Msa1393720:three_prime_UTR
ATGGATAGCTGCAGGGATTG+AGG 0.507871 tig0021758:-14773 Msa1393720:three_prime_UTR
CTGCTGCAGTTCGAAATTCA+TGG 0.508279 tig0021758:-15787 Msa1393720:CDS
CTTCTCTATGTTAACCTGCC+CGG 0.516276 tig0021758:-14899 Msa1393720:three_prime_UTR
ACAGCTTGTTGGTTTGTGCT+TGG 0.518623 tig0021758:+17375 None:intergenic
ATATGAATCTCAGGTAAGAA+TGG 0.520846 tig0021758:-15828 Msa1393720:intron
GAGGGTTAAGGATTGGGATA+GGG 0.521224 tig0021758:-15595 Msa1393720:CDS
AAGAGAATCTCTCTGAATTG+TGG 0.523614 tig0021758:-17873 Msa1393720:CDS
ATTACTTCAAACGATGCTCC+TGG 0.525094 tig0021758:+15295 None:intergenic
AACTTTCTAAGGAGACTAGC+TGG 0.528709 tig0021758:-15211 Msa1393720:CDS
GCAAATATCGTGAAACGTGA+AGG 0.529725 tig0021758:+17798 None:intergenic
ACCATTTATAGCATAGGCTG+TGG 0.530328 tig0021758:-14864 Msa1393720:three_prime_UTR
TGTGCAGTTCTGATTGTAAC+CGG 0.531619 tig0021758:-15373 Msa1393720:intron
TGAGGGTTAAGGATTGGGAT+AGG 0.534539 tig0021758:-15596 Msa1393720:CDS
CTATCCTGCGTTCTTCCCCT+GGG 0.534716 tig0021758:-16194 Msa1393720:CDS
TGCTCTAATCCTAATTGCCC+CGG 0.536471 tig0021758:-16430 Msa1393720:CDS
AGCATTGAATGCAGCCATTG+AGG 0.536896 tig0021758:-15614 Msa1393720:intron
TTACCTTTGTATAACCACTA+AGG 0.537196 tig0021758:+15735 None:intergenic
CTGAATGAAATATCTTGTCC+TGG 0.544198 tig0021758:-15501 Msa1393720:intron
TGTAAGACCATTTATAGCAT+AGG 0.545133 tig0021758:-14870 Msa1393720:three_prime_UTR
TGATATGTCAGGCACTCAGC+TGG 0.545480 tig0021758:-16501 Msa1393720:intron
TTTCTAAAAGAATCGGGAGA+AGG 0.549471 tig0021758:-16378 Msa1393720:CDS
TTCTTCCCCTGGGATAATGC+TGG 0.549567 tig0021758:-16184 Msa1393720:intron
GTCTTACAGATCATCCGGGC+AGG 0.558657 tig0021758:+14885 None:intergenic
CATCATATTCATATATCTTG+TGG 0.560644 tig0021758:+17411 None:intergenic
TCAGAGCAGTTGTTGGATGT+GGG 0.561730 tig0021758:-17548 Msa1393720:intron
TGATTTATTAAAAGCCGAGA+AGG 0.563100 tig0021758:-17132 Msa1393720:CDS
TCCTCTAAATGCATACTCCA+TGG 0.566442 tig0021758:-17186 Msa1393720:CDS
GAGTTGAGTATATTTATCAT+CGG 0.568376 tig0021758:+16240 None:intergenic
GAATGTGTAGAGCTAGTAGG+AGG 0.570661 tig0021758:+17834 None:intergenic
TGTATTACCAGCATTATCCC+AGG 0.576948 tig0021758:+16177 None:intergenic
GGATTGGGATAGGGCACTGG+TGG 0.577488 tig0021758:-15586 Msa1393720:CDS
AACTAGTCGATCACTATCAC+CGG 0.578562 tig0021758:+15354 None:intergenic
TCTGCTTCTTCTTCCCTCGG+TGG 0.585534 tig0021758:-17726 Msa1393720:CDS
AATCGGGAGAAGGCAACCAA+AGG 0.588799 tig0021758:-16368 Msa1393720:CDS
TAATAAATCAAAGAAATGCA+AGG 0.589648 tig0021758:+17145 None:intergenic
TTATCCCAGGGGAAGAACGC+AGG 0.590594 tig0021758:+16190 None:intergenic
TTATCCGTTTGGACAAGTGA+AGG 0.591089 tig0021758:-17967 None:intergenic
AGCAAGCACTGAAAATGCCA+TGG 0.593327 tig0021758:+17169 None:intergenic
AGCGTTTGGATTGACATCTA+GGG 0.594356 tig0021758:-17240 Msa1393720:CDS
AGTCTCTCAGCATTCCATGA+AGG 0.600227 tig0021758:+15331 None:intergenic
CATATTTCGAAGCTCCTGAA+CGG 0.602456 tig0021758:-16458 Msa1393720:CDS
GATTATCATGTACATTGCAA+AGG 0.604945 tig0021758:-16283 Msa1393720:CDS
GTGAGGACCTGAACCACCGA+GGG 0.621442 tig0021758:+17713 None:intergenic
CGAGCTTAAGCAGATAAGAG+AGG 0.621680 tig0021758:-17919 Msa1393720:five_prime_UTR
TCTGGAAATGGATAGCTGCA+GGG 0.627028 tig0021758:-14780 Msa1393720:three_prime_UTR
GAGCTTAAGCAGATAAGAGA+GGG 0.627206 tig0021758:-17918 Msa1393720:five_prime_UTR
GTATTACCAGCATTATCCCA+GGG 0.631989 tig0021758:+16178 None:intergenic
ATGTGTAGAGCTAGTAGGAG+GGG 0.636128 tig0021758:+17836 None:intergenic
AATGGTCTTACAGATCATCC+GGG 0.636488 tig0021758:+14881 None:intergenic
AGACTTTCAAGAGTCATACC+AGG 0.641737 tig0021758:-15313 Msa1393720:CDS
TGTTATGAAGCCTTTAGCCG+AGG 0.647035 tig0021758:-17297 Msa1393720:CDS
TTTGAAGTAATTAAGCAATG+TGG 0.647419 tig0021758:-15283 Msa1393720:CDS
CTAGATGTCAATCCAAACGC+TGG 0.647574 tig0021758:+17242 None:intergenic
CCATTTATAGCATAGGCTGT+GGG 0.648000 tig0021758:-14863 Msa1393720:three_prime_UTR
TGTCCTTAGTGGTTATACAA+AGG 0.659738 tig0021758:-15738 Msa1393720:intron
AATGTGTAGAGCTAGTAGGA+GGG 0.663598 tig0021758:+17835 None:intergenic
GCTGTGGGTCTATATCATAG+AGG 0.710530 tig0021758:-14848 Msa1393720:three_prime_UTR
AGTGAGGACCTGAACCACCG+AGG 0.734066 tig0021758:+17712 None:intergenic
TATTACCAGCATTATCCCAG+GGG 0.766035 tig0021758:+16179 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTATTTTGTTGTTTAT+AGG + tig0021758:17623-17642 None:intergenic 10.0%
!!! CTGTAATATTTTAAAATCTA+AGG - tig0021758:15708-15727 Msa1393720:intron 15.0%
!! CAGAAAAAATATTTCATTAA+GGG + tig0021758:17128-17147 None:intergenic 15.0%
!! ACAGAAAAAATATTTCATTA+AGG + tig0021758:17129-17148 None:intergenic 15.0%
!! TAAATTATGTATTCAATTTC+TGG - tig0021758:17793-17812 Msa1393720:CDS 15.0%
!! TAATAAATCAAAGAAATGCA+AGG + tig0021758:15449-15468 None:intergenic 20.0%
!! TATAAAGTTGACATCTAAAT+AGG - tig0021758:15597-15616 Msa1393720:CDS 20.0%
!! TTTGGAAACTTATAAGAAAA+GGG + tig0021758:16069-16088 None:intergenic 20.0%
!!! TTTTGGAAACTTATAAGAAA+AGG + tig0021758:16070-16089 None:intergenic 20.0%
!! ATAATAATCGAAAAGAAACA+TGG - tig0021758:16494-16513 Msa1393720:intron 20.0%
!! TAGTTACAGAATATGAAAAT+AGG + tig0021758:16901-16920 None:intergenic 20.0%
!! TTGTTATGAGAAGCATAATT+TGG + tig0021758:14832-14851 None:intergenic 25.0%
! ATCTCTAACAAAACAAACAA+TGG + tig0021758:14925-14944 None:intergenic 25.0%
! CATCATATTCATATATCTTG+TGG + tig0021758:15183-15202 None:intergenic 25.0%
! ACTTATTTGATCTTCTTTAC+TGG - tig0021758:15745-15764 Msa1393720:CDS 25.0%
! GAAAAAGGTCTTTATCATAT+AGG + tig0021758:15945-15964 None:intergenic 25.0%
! AGACGTTAAAACTGTATAAT+TGG - tig0021758:15992-16011 Msa1393720:intron 25.0%
! GAGAATATAACACTTTCAAT+TGG + tig0021758:16026-16045 None:intergenic 25.0%
!!! ACCCAATTTTCTAAAAGAAT+CGG - tig0021758:16206-16225 Msa1393720:CDS 25.0%
! GAGTTGAGTATATTTATCAT+CGG + tig0021758:16354-16373 None:intergenic 25.0%
! TTCCAGTTACATATCATTAT+TGG - tig0021758:16466-16485 Msa1393720:CDS 25.0%
! TTCCAATAATGATATGTAAC+TGG + tig0021758:16471-16490 None:intergenic 25.0%
! TGGTCATGACAAATTATATA+TGG + tig0021758:16715-16734 None:intergenic 25.0%
! TGAGATTCATATGAAAGAAA+GGG + tig0021758:16755-16774 None:intergenic 25.0%
!!! TTCAGTTTTTTAGAAAGTTC+TGG + tig0021758:17075-17094 None:intergenic 25.0%
!! TTTGAAGTAATTAAGCAATG+TGG - tig0021758:17308-17327 Msa1393720:CDS 25.0%
! CAATTGTTGAAAACTTTCTA+AGG - tig0021758:17369-17388 Msa1393720:CDS 25.0%
! TTGTACAAAATTCATGTTAC+GGG + tig0021758:17431-17450 None:intergenic 25.0%
! CTTGTACAAAATTCATGTTA+CGG + tig0021758:17432-17451 None:intergenic 25.0%
!! GATTTTCTGTGAAGTAAAAT+GGG + tig0021758:17463-17482 None:intergenic 25.0%
! AAAATCCATCCATATAATAC+AGG - tig0021758:17477-17496 Msa1393720:CDS 25.0%
!! ATGTATTCAATTTCTGGAAA+TGG - tig0021758:17799-17818 Msa1393720:CDS 25.0%
! AACTCAATAAATTGAATGAC+TGG + tig0021758:17852-17871 None:intergenic 25.0%
AACTCACAAAAACAACTATC+TGG + tig0021758:15142-15161 None:intergenic 30.0%
!!! AGTTGTTTTTGTGAGTTCAA+AGG - tig0021758:15145-15164 Msa1393720:three_prime_UTR 30.0%
!!! CAAAAGTTCTAGCTTTTGAT+AGG - tig0021758:15326-15345 Msa1393720:CDS 30.0%
! GTAAACTTAACTTCTGGAAA+TGG - tig0021758:15373-15392 Msa1393720:intron 30.0%
TGATTTATTAAAAGCCGAGA+AGG - tig0021758:15459-15478 Msa1393720:intron 30.0%
!! AAAAATAAGGGCACTAAAAC+AGG + tig0021758:15517-15536 None:intergenic 30.0%
AACATCCGTCTGAAAAATAA+GGG + tig0021758:15529-15548 None:intergenic 30.0%
TTACACATATCTAGATGCTT+TGG + tig0021758:15638-15657 None:intergenic 30.0%
CCTGTAAATGTAAAATTCAG+AGG + tig0021758:15671-15690 None:intergenic 30.0%
! CCTCTGAATTTTACATTTAC+AGG - tig0021758:15668-15687 Msa1393720:intron 30.0%
TTACAGTTGTATCAATGCAT+TGG + tig0021758:15694-15713 None:intergenic 30.0%
ATGAAAACAACTTATGGTCA+TGG + tig0021758:15835-15854 None:intergenic 30.0%
!! GAAGAAGGTTAAGTTGAAAA+AGG + tig0021758:15960-15979 None:intergenic 30.0%
!! GTTTTAACGTCTGATTTCAA+TGG + tig0021758:15984-16003 None:intergenic 30.0%
AGTTTCCAAAATGATATGTC+AGG - tig0021758:16079-16098 Msa1393720:intron 30.0%
!! CCCGATTCTTTTAGAAAATT+GGG + tig0021758:16210-16229 None:intergenic 30.0%
!! CCCAATTTTCTAAAAGAATC+GGG - tig0021758:16207-16226 Msa1393720:CDS 30.0%
!! TCCCGATTCTTTTAGAAAAT+TGG + tig0021758:16211-16230 None:intergenic 30.0%
TGCAATGTACATGATAATCT+TGG + tig0021758:16307-16326 None:intergenic 30.0%
GATTATCATGTACATTGCAA+AGG - tig0021758:16308-16327 Msa1393720:CDS 30.0%
! GGAACATTGATTGAATGAAA+AGG + tig0021758:16560-16579 None:intergenic 30.0%
! CATGAAGACAAGGAATTTTA+AGG + tig0021758:16581-16600 None:intergenic 30.0%
! ATATAATTTGTCATGACCAC+TGG - tig0021758:16716-16735 Msa1393720:intron 30.0%
CTGAGATTCATATGAAAGAA+AGG + tig0021758:16756-16775 None:intergenic 30.0%
CTTTCTTTCATATGAATCTC+AGG - tig0021758:16754-16773 Msa1393720:intron 30.0%
ATATGAATCTCAGGTAAGAA+TGG - tig0021758:16763-16782 Msa1393720:intron 30.0%
AGAATGGCAATTGATAAGTT+TGG - tig0021758:16779-16798 Msa1393720:intron 30.0%
TTACCTTTGTATAACCACTA+AGG + tig0021758:16859-16878 None:intergenic 30.0%
AATATCACCAATTATGACCA+AGG + tig0021758:17191-17210 None:intergenic 30.0%
!!! TTTTTTCTTCATGTGGCAAA+TGG + tig0021758:17334-17353 None:intergenic 30.0%
TTTGCCACATGAAGAAAAAA+TGG - tig0021758:17334-17353 Msa1393720:CDS 30.0%
! ACATGAATTTTGTACAAGTC+AGG - tig0021758:17434-17453 Msa1393720:CDS 30.0%
! GGATTTTCTGTGAAGTAAAA+TGG + tig0021758:17464-17483 None:intergenic 30.0%
TAGTGTCTTCCTGTATTATA+TGG + tig0021758:17489-17508 None:intergenic 30.0%
ATATCTTCAAGGACTATAGA+AGG + tig0021758:17672-17691 None:intergenic 30.0%
ACATAGAGAAGATATCTTCA+AGG + tig0021758:17683-17702 None:intergenic 30.0%
TGTAAGACCATTTATAGCAT+AGG - tig0021758:17721-17740 Msa1393720:CDS 30.0%
AAAATGACTGCAAATGATTC+TGG - tig0021758:17937-17956 Msa1393720:five_prime_UTR 30.0%
AAGAGAATCTCTCTGAATTG+TGG - tig0021758:14718-14737 Msa1393720:three_prime_UTR 35.0%
TCTCTGAATTGTGGACAATT+GGG - tig0021758:14727-14746 Msa1393720:three_prime_UTR 35.0%
TCACGATATTTGCTTCTTCA+TGG - tig0021758:14803-14822 Msa1393720:three_prime_UTR 35.0%
GTATAGATAGAAGAAGAGTG+AGG + tig0021758:14898-14917 None:intergenic 35.0%
ATGTGGGATCAAAGAAGAAA+AGG - tig0021758:15059-15078 Msa1393720:three_prime_UTR 35.0%
AAGAAAAGGAAAGAGATAGC+TGG - tig0021758:15073-15092 Msa1393720:three_prime_UTR 35.0%
AATCCATGCAACAGAATCAT+AGG + tig0021758:15247-15266 None:intergenic 35.0%
ATGATTCTGTTGCATGGATT+CGG - tig0021758:15247-15266 Msa1393720:CDS 35.0%
! ATTTTTCCAAGAGAAAACCG+AGG + tig0021758:15275-15294 None:intergenic 35.0%
! AACTTTTGAACATGTAGCCT+CGG + tig0021758:15314-15333 None:intergenic 35.0%
! TCTAGGGTAAACTTAACTTC+TGG - tig0021758:15367-15386 Msa1393720:CDS 35.0%
!!! TAGTGCCCTTATTTTTCAGA+CGG - tig0021758:15521-15540 Msa1393720:CDS 35.0%
CAACATCCGTCTGAAAAATA+AGG + tig0021758:15530-15549 None:intergenic 35.0%
!! TTTTTCAGACGGATGTTGTT+AGG - tig0021758:15532-15551 Msa1393720:CDS 35.0%
! TAGGTGACTTTTGTGAATCT+AGG - tig0021758:15551-15570 Msa1393720:CDS 35.0%
GAGAGGATGAAAACAACTTA+TGG + tig0021758:15841-15860 None:intergenic 35.0%
!! GTTGTTTTCATAAGCTATCC+TGG + tig0021758:15863-15882 None:intergenic 35.0%
TCTGATTTCAATGGAGAAGA+AGG + tig0021758:15975-15994 None:intergenic 35.0%
AGAAAATTGGGTGTAGTAAG+TGG + tig0021758:16198-16217 None:intergenic 35.0%
! TTTCTAAAAGAATCGGGAGA+AGG - tig0021758:16213-16232 Msa1393720:CDS 35.0%
AACTGTTGTCTTCTTTCCTT+TGG + tig0021758:16242-16261 None:intergenic 35.0%
ATCCAATTGTTGGACTTTAC+AGG - tig0021758:16274-16293 Msa1393720:CDS 35.0%
AACCTGTAAAGTCCAACAAT+TGG + tig0021758:16279-16298 None:intergenic 35.0%
TATGTATGGACATGAAGACA+AGG + tig0021758:16591-16610 None:intergenic 35.0%
CAGCTATGTGAGGAAAAATA+TGG - tig0021758:16613-16632 Msa1393720:intron 35.0%
TTCATGGTATGATCCAAAAC+AGG - tig0021758:16820-16839 Msa1393720:intron 35.0%
TGTCCTTAGTGGTTATACAA+AGG - tig0021758:16853-16872 Msa1393720:intron 35.0%
TCAAATAGTCTGTGCTTACT+GGG - tig0021758:16950-16969 Msa1393720:intron 35.0%
CTGAATGAAATATCTTGTCC+TGG - tig0021758:17090-17109 Msa1393720:intron 35.0%
CTGTTGTGTAAAGATCTAAC+TGG - tig0021758:17145-17164 Msa1393720:CDS 35.0%
AGTTGAACCTTGGTCATAAT+TGG - tig0021758:17181-17200 Msa1393720:CDS 35.0%
!!! TCCTCCATTTTTTCTTCATG+TGG + tig0021758:17341-17360 None:intergenic 35.0%
! AAATTCATGTTACGGGTTGT+AGG + tig0021758:17424-17443 None:intergenic 35.0%
GTCTTCCTGTATTATATGGA+TGG + tig0021758:17485-17504 None:intergenic 35.0%
AGACCAGAAAATGGAAAGAT+TGG - tig0021758:17584-17603 Msa1393720:intron 35.0%
! CTTCCAATCTTTCCATTTTC+TGG + tig0021758:17590-17609 None:intergenic 35.0%
GAAAATGGAAAGATTGGAAG+TGG - tig0021758:17590-17609 Msa1393720:intron 35.0%
! AAATGGTCTTACAGATCATC+CGG + tig0021758:17714-17733 None:intergenic 35.0%
!! AGGTACTGAATGAGAGTTAG+TGG - tig0021758:14644-14663 Msa1393720:three_prime_UTR 40.0%
GAGCTTAAGCAGATAAGAGA+GGG - tig0021758:14673-14692 Msa1393720:three_prime_UTR 40.0%
CTCTCTGAATTGTGGACAAT+TGG - tig0021758:14726-14745 Msa1393720:three_prime_UTR 40.0%
AATGTGTAGAGCTAGTAGGA+GGG + tig0021758:14759-14778 None:intergenic 40.0%
CATGAATGTGTAGAGCTAGT+AGG + tig0021758:14763-14782 None:intergenic 40.0%
GCAAATATCGTGAAACGTGA+AGG + tig0021758:14796-14815 None:intergenic 40.0%
AGAAGAAGCAGAAGCAATGA+AGG + tig0021758:14857-14876 None:intergenic 40.0%
! TTGTTGTTCAGAGCAGTTGT+TGG - tig0021758:15036-15055 Msa1393720:three_prime_UTR 40.0%
AGAGATAGCTGGAATAGATC+AGG - tig0021758:15084-15103 Msa1393720:three_prime_UTR 40.0%
TCTGGATCAGCTAATAGCTT+TGG + tig0021758:15124-15143 None:intergenic 40.0%
TTGTGCTTGGGAATGAGATT+CGG + tig0021758:15206-15225 None:intergenic 40.0%
TTGCCTATGATTCTGTTGCA+TGG - tig0021758:15241-15260 Msa1393720:CDS 40.0%
AGCGTTTGGATTGACATCTA+GGG - tig0021758:15351-15370 Msa1393720:CDS 40.0%
TCCTCTAAATGCATACTCCA+TGG - tig0021758:15405-15424 Msa1393720:intron 40.0%
!! AAGTTGTTTTCATCCTCTCC+AGG - tig0021758:15842-15861 Msa1393720:intron 40.0%
TTTCATAAGCTATCCTGGAG+AGG + tig0021758:15858-15877 None:intergenic 40.0%
GAAAAGGGACTACAATGTAG+AGG + tig0021758:16054-16073 None:intergenic 40.0%
! GAGTGCCTGACATATCATTT+TGG + tig0021758:16087-16106 None:intergenic 40.0%
CATATTTCGAAGCTCCTGAA+CGG - tig0021758:16133-16152 Msa1393720:intron 40.0%
ATATTTCGAAGCTCCTGAAC+GGG - tig0021758:16134-16153 Msa1393720:intron 40.0%
TAAGTGGAGCAAAAATTGCC+GGG + tig0021758:16182-16201 None:intergenic 40.0%
GTAAGTGGAGCAAAAATTGC+CGG + tig0021758:16183-16202 None:intergenic 40.0%
GTCACACGAAATCCAATTGT+TGG - tig0021758:16264-16283 Msa1393720:CDS 40.0%
TATTACCAGCATTATCCCAG+GGG + tig0021758:16415-16434 None:intergenic 40.0%
GTATTACCAGCATTATCCCA+GGG + tig0021758:16416-16435 None:intergenic 40.0%
TGTATTACCAGCATTATCCC+AGG + tig0021758:16417-16436 None:intergenic 40.0%
CTCACATAGCTGACTATGTA+TGG + tig0021758:16605-16624 None:intergenic 40.0%
CATACATAGTCAGCTATGTG+AGG - tig0021758:16603-16622 Msa1393720:intron 40.0%
TGACCACTGGACTAATGTAA+TGG - tig0021758:16729-16748 Msa1393720:intron 40.0%
CTCAAATAGTCTGTGCTTAC+TGG - tig0021758:16949-16968 Msa1393720:intron 40.0%
TATGACCAAGGTTCAACTTG+AGG + tig0021758:17179-17198 None:intergenic 40.0%
! TGTGCAGTTCTGATTGTAAC+CGG - tig0021758:17218-17237 Msa1393720:CDS 40.0%
AACTAGTCGATCACTATCAC+CGG + tig0021758:17240-17259 None:intergenic 40.0%
AGACTTTCAAGAGTCATACC+AGG - tig0021758:17278-17297 Msa1393720:CDS 40.0%
ATTACTTCAAACGATGCTCC+TGG + tig0021758:17299-17318 None:intergenic 40.0%
GCCACATGAAGAAAAAATGG+AGG - tig0021758:17337-17356 Msa1393720:CDS 40.0%
! AACTTTCTAAGGAGACTAGC+TGG - tig0021758:17380-17399 Msa1393720:CDS 40.0%
CACTAGTGCAATTGAACAGA+TGG - tig0021758:17504-17523 Msa1393720:CDS 40.0%
! AATGGTCTTACAGATCATCC+GGG + tig0021758:17713-17732 None:intergenic 40.0%
ACCATTTATAGCATAGGCTG+TGG - tig0021758:17727-17746 Msa1393720:CDS 40.0%
CCATTTATAGCATAGGCTGT+GGG - tig0021758:17728-17747 Msa1393720:CDS 40.0%
CGAGCTTAAGCAGATAAGAG+AGG - tig0021758:14672-14691 Msa1393720:three_prime_UTR 45.0%
ATGTGTAGAGCTAGTAGGAG+GGG + tig0021758:14758-14777 None:intergenic 45.0%
GAATGTGTAGAGCTAGTAGG+AGG + tig0021758:14760-14779 None:intergenic 45.0%
TTCAGAGCAGTTGTTGGATG+TGG - tig0021758:15042-15061 Msa1393720:three_prime_UTR 45.0%
TCAGAGCAGTTGTTGGATGT+GGG - tig0021758:15043-15062 Msa1393720:three_prime_UTR 45.0%
!! CAGCTTGTTGGTTTGTGCTT+GGG + tig0021758:15218-15237 None:intergenic 45.0%
!! ACAGCTTGTTGGTTTGTGCT+TGG + tig0021758:15219-15238 None:intergenic 45.0%
CATAGGCAAACACAGCTTGT+TGG + tig0021758:15230-15249 None:intergenic 45.0%
TGTTATGAAGCCTTTAGCCG+AGG - tig0021758:15294-15313 Msa1393720:CDS 45.0%
CTAGATGTCAATCCAAACGC+TGG + tig0021758:15352-15371 None:intergenic 45.0%
CAGCGTTTGGATTGACATCT+AGG - tig0021758:15350-15369 Msa1393720:CDS 45.0%
GCCATGGAGTATGCATTTAG+AGG + tig0021758:15409-15428 None:intergenic 45.0%
!! AGCAAGCACTGAAAATGCCA+TGG + tig0021758:15425-15444 None:intergenic 45.0%
! AGCCGAGAAGGCAATTTTAG+TGG - tig0021758:15471-15490 Msa1393720:intron 45.0%
!! GCCGAGAAGGCAATTTTAGT+GGG - tig0021758:15472-15491 Msa1393720:intron 45.0%
CCCCACTAAAATTGCCTTCT+CGG + tig0021758:15476-15495 None:intergenic 45.0%
!! CCGAGAAGGCAATTTTAGTG+GGG - tig0021758:15473-15492 Msa1393720:intron 45.0%
TGCTCTAATCCTAATTGCCC+CGG - tig0021758:16161-16180 Msa1393720:intron 45.0%
CAAAAATTGCCGGGGCAATT+AGG + tig0021758:16173-16192 None:intergenic 45.0%
AAGTGGAGCAAAAATTGCCG+GGG + tig0021758:16181-16200 None:intergenic 45.0%
CTGCTGCAGTTCGAAATTCA+TGG - tig0021758:16804-16823 Msa1393720:intron 45.0%
! ACATGCTCAGAGACCTGTTT+TGG + tig0021758:16836-16855 None:intergenic 45.0%
! AGCATTGAATGCAGCCATTG+AGG - tig0021758:16977-16996 Msa1393720:intron 45.0%
GCATTGAATGCAGCCATTGA+GGG - tig0021758:16978-16997 Msa1393720:intron 45.0%
AATGCAGCCATTGAGGGTTA+AGG - tig0021758:16984-17003 Msa1393720:intron 45.0%
AGCCATTGAGGGTTAAGGAT+TGG - tig0021758:16989-17008 Msa1393720:intron 45.0%
GCCATTGAGGGTTAAGGATT+GGG - tig0021758:16990-17009 Msa1393720:intron 45.0%
TCCCAATCCTTAACCCTCAA+TGG + tig0021758:16994-17013 None:intergenic 45.0%
TGAGGGTTAAGGATTGGGAT+AGG - tig0021758:16995-17014 Msa1393720:intron 45.0%
GAGGGTTAAGGATTGGGATA+GGG - tig0021758:16996-17015 Msa1393720:intron 45.0%
ATTCACTGCTGCAATGCTTC+TGG - tig0021758:17029-17048 Msa1393720:intron 45.0%
TAAGGGTCAGTGAAATCACC+AGG + tig0021758:17111-17130 None:intergenic 45.0%
!! CATAGCCTCAAGTTGAACCT+TGG - tig0021758:17171-17190 Msa1393720:CDS 45.0%
! GTGATCGACTAGTTCCTTCA+TGG - tig0021758:17246-17265 Msa1393720:CDS 45.0%
AGTCTCTCAGCATTCCATGA+AGG + tig0021758:17263-17282 None:intergenic 45.0%
AGCATGACGAGACCAGAAAA+TGG - tig0021758:17575-17594 Msa1393720:intron 45.0%
CTTCTCTATGTTAACCTGCC+CGG - tig0021758:17692-17711 Msa1393720:intron 45.0%
CCCACAGCCTATGCTATAAA+TGG + tig0021758:17731-17750 None:intergenic 45.0%
GCTGTGGGTCTATATCATAG+AGG - tig0021758:17743-17762 Msa1393720:CDS 45.0%
! TTCTGGAAATGGATAGCTGC+AGG - tig0021758:17810-17829 Msa1393720:CDS 45.0%
TCTGGAAATGGATAGCTGCA+GGG - tig0021758:17811-17830 Msa1393720:CDS 45.0%
!! TATACAAAAATATATATTTA+AGG - tig0021758:17894-17913 Msa1393720:exon 5.0%
!! ATACAAAAATATATATTTAA+GGG - tig0021758:17895-17914 Msa1393720:exon 5.0%
GCTTCTGCTTCTTCTTCCCT+CGG - tig0021758:14862-14881 Msa1393720:three_prime_UTR 50.0%
GAACATGTAGCCTCGGCTAA+AGG + tig0021758:15307-15326 None:intergenic 50.0%
! GCTTTTGATAGGCCAGCGTT+TGG - tig0021758:15337-15356 Msa1393720:CDS 50.0%
TGATATGTCAGGCACTCAGC+TGG - tig0021758:16090-16109 Msa1393720:intron 50.0%
ATTAGAGCAGCAACCCGTTC+AGG + tig0021758:16150-16169 None:intergenic 50.0%
AATCGGGAGAAGGCAACCAA+AGG - tig0021758:16223-16242 Msa1393720:CDS 50.0%
TTCTTCCCCTGGGATAATGC+TGG - tig0021758:16407-16426 Msa1393720:CDS 50.0%
GGGCCATTACATTAGTCCAG+TGG + tig0021758:16735-16754 None:intergenic 50.0%
GTCTCTGAGCATGTCCTTAG+TGG - tig0021758:16842-16861 Msa1393720:intron 50.0%
TAAGGATTGGGATAGGGCAC+TGG - tig0021758:17002-17021 Msa1393720:intron 50.0%
ATGGATAGCTGCAGGGATTG+AGG - tig0021758:17818-17837 Msa1393720:CDS 50.0%
TCTGCTTCTTCTTCCCTCGG+TGG - tig0021758:14865-14884 Msa1393720:three_prime_UTR 55.0%
TCTTCTTCCCTCGGTGGTTC+AGG - tig0021758:14871-14890 Msa1393720:three_prime_UTR 55.0%
GTTGCATGGATTCGGAGCCT+CGG - tig0021758:15255-15274 Msa1393720:CDS 55.0%
! TCGGAGCCTCGGTTTTCTCT+TGG - tig0021758:15266-15285 Msa1393720:CDS 55.0%
TCTATCCTGCGTTCTTCCCC+TGG - tig0021758:16396-16415 Msa1393720:CDS 55.0%
CTATCCTGCGTTCTTCCCCT+GGG - tig0021758:16397-16416 Msa1393720:CDS 55.0%
TTATCCCAGGGGAAGAACGC+AGG + tig0021758:16404-16423 None:intergenic 55.0%
TGCTGCAATGCTTCTGGACG+AGG - tig0021758:17035-17054 Msa1393720:intron 55.0%
GTCTTACAGATCATCCGGGC+AGG + tig0021758:17709-17728 None:intergenic 55.0%
GTGAGGACCTGAACCACCGA+GGG + tig0021758:14881-14900 None:intergenic 60.0%
AGTGAGGACCTGAACCACCG+AGG + tig0021758:14882-14901 None:intergenic 60.0%
!! GGATTGGGATAGGGCACTGG+TGG - tig0021758:17005-17024 Msa1393720:intron 60.0%
Chromosome Type Strat End Strand Name
tig0021758 gene 14633 17980 14633 ID=Msa1393720;Name=Msa1393720
tig0021758 mRNA 14633 17980 14633 ID=Msa1393720-mRNA-1;Parent=Msa1393720;Name=Msa1393720-mRNA-1;_AED=0.01;_eAED=0.01;_QI=82|1|1|1|1|1|6|541|462
tig0021758 exon 14633 15388 14633 ID=Msa1393720-mRNA-1:exon:13982;Parent=Msa1393720-mRNA-1
tig0021758 exon 15502 15622 15502 ID=Msa1393720-mRNA-1:exon:13981;Parent=Msa1393720-mRNA-1
tig0021758 exon 15739 15837 15739 ID=Msa1393720-mRNA-1:exon:13980;Parent=Msa1393720-mRNA-1
tig0021758 exon 16185 16512 16185 ID=Msa1393720-mRNA-1:exon:13979;Parent=Msa1393720-mRNA-1
tig0021758 exon 17119 17566 17119 ID=Msa1393720-mRNA-1:exon:13978;Parent=Msa1393720-mRNA-1
tig0021758 exon 17721 17980 17721 ID=Msa1393720-mRNA-1:exon:13977;Parent=Msa1393720-mRNA-1
tig0021758 five_prime_UTR 17899 17980 17899 ID=Msa1393720-mRNA-1:five_prime_utr;Parent=Msa1393720-mRNA-1
tig0021758 CDS 17721 17898 17721 ID=Msa1393720-mRNA-1:cds;Parent=Msa1393720-mRNA-1
tig0021758 CDS 17119 17566 17119 ID=Msa1393720-mRNA-1:cds;Parent=Msa1393720-mRNA-1
tig0021758 CDS 16185 16512 16185 ID=Msa1393720-mRNA-1:cds;Parent=Msa1393720-mRNA-1
tig0021758 CDS 15739 15837 15739 ID=Msa1393720-mRNA-1:cds;Parent=Msa1393720-mRNA-1
tig0021758 CDS 15502 15622 15502 ID=Msa1393720-mRNA-1:cds;Parent=Msa1393720-mRNA-1
tig0021758 CDS 15174 15388 15174 ID=Msa1393720-mRNA-1:cds;Parent=Msa1393720-mRNA-1
tig0021758 three_prime_UTR 14633 15173 14633 ID=Msa1393720-mRNA-1:three_prime_utr;Parent=Msa1393720-mRNA-1
Gene Sequence

>Msa1393720

ATGAAGAGAATCTCTCTGAATTGTGGACAATTGGGTCTCACCCCTCCTACTAGCTCTACACATTCATGTAATAAGCGTCCTTCACGTTTCACGATATTTGCTTCTTCATGGCTTCCAAATTATGCTTCTCATAACAACACCTTCATTGCTTCTGCTTCTTCTTCCCTCGGTGGTTCAGAGCAGTTGTTGGATGTGGGATCAAAGAAGAAAAGGAAAGAGATAGCTGGAATAGATCAGGATGAATTAGTAGATCCAAAGCTATTAGCTGATCCAGATAGTTGTTTTTGTGAGTTCAAAGGAGTGCATATACACCACAAGATATATGAATATGATGCCGAATCTCATTCCCAAGCACAAACCAACAAGCTGTGTTTGCCTATGATTCTGTTGCATGGATTCGGAGCCTCGGTTTTCTCTTGGAAAAATGTTATGAAGCCTTTAGCCGAGGCTACATGTTCAAAAGTTCTAGCTTTTGATAGGCCAGCGTTTGGATTGACATCTAGGGTAAACTTAACTTCTGGAAATGGAGACACAAATCCTCTAAATGCATACTCCATGGCATTTTCAGTGCTTGCTACCTTGCATTTCTTTGATTTATTAAAAGCCGAGAAGGCAATTTTAGTGGGGCACTCAGCTGGTTCACTTGTAGCAGTTAACACATATTTCGAAGCTCCTGAACGGGTTGCTGCTCTAATCCTAATTGCCCCGGCAATTTTTGCTCCACTTACTACACCCAATTTTCTAAAAGAATCGGGAGAAGGCAACCAAAGGAAAGAAGACAACAGTTCTGTCACACGAAATCCAATTGTTGGACTTTACAGGTTGCTGTCCAAGATTATCATGTACATTGCAAAGGCAATAGCAGAGATGATGAAGCCGATGATAAATATACTCAACTCTCTCTATAAGAAACTGTTATCATCTATCCTGCGTTCTTCCCCTGGGATAATGCTGGTAAGAATGGCAATTGATAAGTTTGGTGCTGCTGCAGTTCGAAATTCATGGTATGATCCAAAACAGGTCTCTGAGCATGTCCTTAGTGGTTATACAAAGCCATTGAGGGTTAAGGATTGGGATAGGGCACTGGTGGAATTCACTGCTGCAATGCTTCTGGACGAGGAATCTAATACAAAGCCAGAACTTTCTAAAAAACTGAATGAAATATCTTGTCCTGTTCTGATTGTAACCGGTGATAGTGATCGACTAGTTCCTTCATGGAATGCTGAGAGACTTTCAAGAGTCATACCAGGAGCATCGTTTGAAGTAATTAAGCAATGTGGCCATTTGCCACATGAAGAAAAAATGGAGGAGTTTATTTCAATTGTTGAAAACTTTCTAAGGAGACTAGCTGGTGATTCAAACAAACAAGACCTACAACCCGTAACATGA
Protein sequence

>Msa1393720

MKRISLNCGQLGLTPPTSSTHSCNKRPSRFTIFASSWLPNYASHNNTFIASASSSLGGSEQLLDVGSKKKRKEIAGIDQDELVDPKLLADPDSCFCEFKGVHIHHKIYEYDAESHSQAQTNKLCLPMILLHGFGASVFSWKNVMKPLAEATCSKVLAFDRPAFGLTSRVNLTSGNGDTNPLNAYSMAFSVLATLHFFDLLKAEKAILVGHSAGSLVAVNTYFEAPERVAALILIAPAIFAPLTTPNFLKESGEGNQRKEDNSSVTRNPIVGLYRLLSKIIMYIAKAIAEMMKPMINILNSLYKKLLSSILRSSPGIMLVRMAIDKFGAAAVRNSWYDPKQVSEHVLSGYTKPLRVKDWDRALVEFTAAMLLDEESNTKPELSKKLNEISCPVLIVTGDSDRLVPSWNAERLSRVIPGASFEVIKQCGHLPHEEKMEEFISIVENFLRRLAGDSNKQDLQPVT*