AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27987


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27987.t1 MTR_8g089610 96.040 202 8 0 1 202 1 202 2.22e-120 339
MS.gene27987.t1 MTR_3g086230 64.390 205 59 10 1 201 1 195 1.03e-63 196
MS.gene27987.t1 MTR_3g086230 73.333 45 11 1 85 129 154 197 3.13e-13 65.9
MS.gene27987.t1 MTR_2g046810 74.172 151 17 4 10 158 1 131 1.31e-55 173
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27987.t1 AT1G67740 62.420 157 55 2 47 201 32 186 3.45e-49 159
MS.gene27987.t1 AT1G67740 50.769 65 30 2 140 202 51 115 3.06e-12 63.2

Find 56 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTTTGTAATGAGCTTATT+TGG 0.218893 4.4:-11725640 None:intergenic
GGTGGTGGGTTAGCAGCTTC+AGG 0.222861 4.4:+11725976 MS.gene27987:CDS
CAAGCTATGGCTGGAATTAA+TGG 0.225855 4.4:-11725868 None:intergenic
AAGCTGCTGGTAGTGATAAT+AGG 0.242204 4.4:+11726049 MS.gene27987:CDS
TGGTAGCACCAGCTATTCTT+TGG 0.258357 4.4:+11726091 MS.gene27987:CDS
AAAGGAGTTATCATTGGTTT+AGG 0.300660 4.4:+11725949 MS.gene27987:CDS
AACCGTATGCGTAACGATAA+AGG 0.307985 4.4:+11725931 MS.gene27987:CDS
ACTGTCTCATTCTGTTGATT+TGG 0.310184 4.4:-11726146 None:intergenic
GTTATCATTGGTTTAGGTCT+TGG 0.319441 4.4:+11725955 MS.gene27987:CDS
CGTTACGCATACGGTTGATT+TGG 0.341217 4.4:-11725924 None:intergenic
AGCTGCTGGTAGTGATAATA+GGG 0.355815 4.4:+11726050 MS.gene27987:CDS
GGTTTGTAATGAGCTTATTT+GGG 0.370406 4.4:-11725639 None:intergenic
CCTGTTGTGGCAATTCCCTT+TGG 0.390126 4.4:-11725694 None:intergenic
GGTAGCACCAGCTATTCTTT+GGG 0.391322 4.4:+11726092 MS.gene27987:CDS
GTGGTGGGTTAGCAGCTTCA+GGG 0.392842 4.4:+11725977 MS.gene27987:CDS
CTCCTTTATCGTTACGCATA+CGG 0.399509 4.4:-11725933 None:intergenic
ATTGGTTTAGGTCTTGGTGG+TGG 0.402929 4.4:+11725961 MS.gene27987:CDS
AACGATAAAGGAGTTATCAT+TGG 0.410305 4.4:+11725943 MS.gene27987:CDS
TTGAGGGAAATGAGGGAAGT+GGG 0.420587 4.4:-11725661 None:intergenic
GGGTCAGCTTCTCTTGTTTG+TGG 0.421686 4.4:+11726071 MS.gene27987:CDS
CTTTGGAAGAAGGTTCTTGA+GGG 0.435326 4.4:-11725677 None:intergenic
CATTAATTCCAGCCATAGCT+TGG 0.438157 4.4:+11725869 MS.gene27987:CDS
AGCTAGTGCTGTTCCTGCTA+TGG 0.441899 4.4:-11725737 None:intergenic
CAAGGACTTTGAGTTGATCA+TGG 0.443749 4.4:-11725587 None:intergenic
TGGCAATTCCCTTTGGAAGA+AGG 0.444343 4.4:-11725687 None:intergenic
CTCAAGAACCTTCTTCCAAA+GGG 0.458123 4.4:+11725679 MS.gene27987:CDS
ATCATTGGTTTAGGTCTTGG+TGG 0.458537 4.4:+11725958 MS.gene27987:CDS
CCAAAGGGAATTGCCACAAC+AGG 0.466016 4.4:+11725694 MS.gene27987:CDS
TTATTTGGGTTGTGGAACTT+TGG 0.470489 4.4:-11725625 None:intergenic
TTGGTTTAGGTCTTGGTGGT+GGG 0.471445 4.4:+11725962 MS.gene27987:CDS
TAATGAGCTTATTTGGGTTG+TGG 0.472958 4.4:-11725633 None:intergenic
ATTAATTCCAGCCATAGCTT+GGG 0.480687 4.4:+11725870 MS.gene27987:CDS
CTGTTGATTTGGTTAAGAGC+AGG 0.484319 4.4:-11726135 None:intergenic
TGGTGAACTAGTAGTGTTCA+AGG 0.485829 4.4:-11725605 None:intergenic
TAGTTTAATGTTTCCTGTTG+TGG 0.486608 4.4:-11725707 None:intergenic
TAAAGAACCCAAAGAATAGC+TGG 0.496379 4.4:-11726099 None:intergenic
CCTCAAGAACCTTCTTCCAA+AGG 0.498567 4.4:+11725678 MS.gene27987:CDS
AAGGTTCTTGAGGGAAATGA+GGG 0.499781 4.4:-11725668 None:intergenic
ATAGCAGGAACAGCACTAGC+TGG 0.506700 4.4:+11725739 MS.gene27987:CDS
GATCATGGTCATTGCAATGA+TGG 0.513443 4.4:-11725572 None:intergenic
CTTGAGGGAAATGAGGGAAG+TGG 0.514770 4.4:-11725662 None:intergenic
GCTGCAATTGCTGAAGCTGC+TGG 0.514881 4.4:+11726036 MS.gene27987:CDS
TACAGAACCCAAGCTATGGC+TGG 0.516463 4.4:-11725877 None:intergenic
TGAGGGAAATGAGGGAAGTG+GGG 0.522481 4.4:-11725660 None:intergenic
TGCTGCAAAAGCTGCATCAC+AGG 0.523420 4.4:-11725785 None:intergenic
GAAGGTTCTTGAGGGAAATG+AGG 0.523923 4.4:-11725669 None:intergenic
AGATGCAATGGCTAGTGAAA+TGG 0.532397 4.4:+11726014 MS.gene27987:CDS
CGGTTGATTTGGTTAAGTGC+TGG 0.533998 4.4:-11725913 None:intergenic
CCTTTGGAAGAAGGTTCTTG+AGG 0.537577 4.4:-11725678 None:intergenic
AAACTATCAACTTCCATAGC+AGG 0.544376 4.4:+11725724 MS.gene27987:CDS
GTTGTACAGAACCCAAGCTA+TGG 0.571265 4.4:-11725881 None:intergenic
CAAATAGCTGAAATAGCAGA+AGG 0.608735 4.4:+11725811 MS.gene27987:CDS
TGTGTCAACACAAGATGCAA+TGG 0.611402 4.4:+11726002 MS.gene27987:CDS
GCTGCTGGTAGTGATAATAG+GGG 0.720408 4.4:+11726051 MS.gene27987:CDS
GTAACAGAAGTGCTAAACCA+CGG 0.728265 4.4:-11725846 None:intergenic
GAAGGATCATCAGACAACCG+TGG 0.746201 4.4:+11725829 MS.gene27987:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACGATAAAGGAGTTATCAT+TGG + chr4.4:11725943-11725962 MS.gene27987:CDS 30.0%
GGTTTGTAATGAGCTTATTT+GGG - chr4.4:11725642-11725661 None:intergenic 30.0%
TAGTTTAATGTTTCCTGTTG+TGG - chr4.4:11725710-11725729 None:intergenic 30.0%
!! AAAGGAGTTATCATTGGTTT+AGG + chr4.4:11725949-11725968 MS.gene27987:CDS 30.0%
AAACTATCAACTTCCATAGC+AGG + chr4.4:11725724-11725743 MS.gene27987:CDS 35.0%
ATTAATTCCAGCCATAGCTT+GGG + chr4.4:11725870-11725889 MS.gene27987:CDS 35.0%
CAAATAGCTGAAATAGCAGA+AGG + chr4.4:11725811-11725830 MS.gene27987:CDS 35.0%
GGGTTTGTAATGAGCTTATT+TGG - chr4.4:11725643-11725662 None:intergenic 35.0%
TAAAGAACCCAAAGAATAGC+TGG - chr4.4:11726102-11726121 None:intergenic 35.0%
! ACTGTCTCATTCTGTTGATT+TGG - chr4.4:11726149-11726168 None:intergenic 35.0%
!! GTTATCATTGGTTTAGGTCT+TGG + chr4.4:11725955-11725974 MS.gene27987:CDS 35.0%
!! TAATGAGCTTATTTGGGTTG+TGG - chr4.4:11725636-11725655 None:intergenic 35.0%
!! TTATTTGGGTTGTGGAACTT+TGG - chr4.4:11725628-11725647 None:intergenic 35.0%
AACCGTATGCGTAACGATAA+AGG + chr4.4:11725931-11725950 MS.gene27987:CDS 40.0%
AAGGTTCTTGAGGGAAATGA+GGG - chr4.4:11725671-11725690 None:intergenic 40.0%
AGATGCAATGGCTAGTGAAA+TGG + chr4.4:11726014-11726033 MS.gene27987:CDS 40.0%
CAAGCTATGGCTGGAATTAA+TGG - chr4.4:11725871-11725890 None:intergenic 40.0%
CATTAATTCCAGCCATAGCT+TGG + chr4.4:11725869-11725888 MS.gene27987:CDS 40.0%
CTCAAGAACCTTCTTCCAAA+GGG + chr4.4:11725679-11725698 MS.gene27987:CDS 40.0%
CTCCTTTATCGTTACGCATA+CGG - chr4.4:11725936-11725955 None:intergenic 40.0%
GATCATGGTCATTGCAATGA+TGG - chr4.4:11725575-11725594 None:intergenic 40.0%
TGGTGAACTAGTAGTGTTCA+AGG - chr4.4:11725608-11725627 None:intergenic 40.0%
TGTGTCAACACAAGATGCAA+TGG + chr4.4:11726002-11726021 MS.gene27987:CDS 40.0%
! AAGCTGCTGGTAGTGATAAT+AGG + chr4.4:11726049-11726068 MS.gene27987:CDS 40.0%
! AGCTGCTGGTAGTGATAATA+GGG + chr4.4:11726050-11726069 MS.gene27987:CDS 40.0%
! CTTTGGAAGAAGGTTCTTGA+GGG - chr4.4:11725680-11725699 None:intergenic 40.0%
!! ATCATTGGTTTAGGTCTTGG+TGG + chr4.4:11725958-11725977 MS.gene27987:CDS 40.0%
!! CAAGGACTTTGAGTTGATCA+TGG - chr4.4:11725590-11725609 None:intergenic 40.0%
!! CTGTTGATTTGGTTAAGAGC+AGG - chr4.4:11726138-11726157 None:intergenic 40.0%
!! GTAACAGAAGTGCTAAACCA+CGG - chr4.4:11725849-11725868 None:intergenic 40.0%
CCTCAAGAACCTTCTTCCAA+AGG + chr4.4:11725678-11725697 MS.gene27987:CDS 45.0%
CGTTACGCATACGGTTGATT+TGG - chr4.4:11725927-11725946 None:intergenic 45.0%
GAAGGTTCTTGAGGGAAATG+AGG - chr4.4:11725672-11725691 None:intergenic 45.0%
GGTAGCACCAGCTATTCTTT+GGG + chr4.4:11726092-11726111 MS.gene27987:CDS 45.0%
GTTGTACAGAACCCAAGCTA+TGG - chr4.4:11725884-11725903 None:intergenic 45.0%
TGGTAGCACCAGCTATTCTT+TGG + chr4.4:11726091-11726110 MS.gene27987:CDS 45.0%
TTGAGGGAAATGAGGGAAGT+GGG - chr4.4:11725664-11725683 None:intergenic 45.0%
! ATTGGTTTAGGTCTTGGTGG+TGG + chr4.4:11725961-11725980 MS.gene27987:CDS 45.0%
! CCTTTGGAAGAAGGTTCTTG+AGG - chr4.4:11725681-11725700 None:intergenic 45.0%
! GCTGCTGGTAGTGATAATAG+GGG + chr4.4:11726051-11726070 MS.gene27987:CDS 45.0%
! TGGCAATTCCCTTTGGAAGA+AGG - chr4.4:11725690-11725709 None:intergenic 45.0%
! TTGGTTTAGGTCTTGGTGGT+GGG + chr4.4:11725962-11725981 MS.gene27987:CDS 45.0%
!! CGGTTGATTTGGTTAAGTGC+TGG - chr4.4:11725916-11725935 None:intergenic 45.0%
ATAGCAGGAACAGCACTAGC+TGG + chr4.4:11725739-11725758 MS.gene27987:CDS 50.0%
CCAAAGGGAATTGCCACAAC+AGG + chr4.4:11725694-11725713 MS.gene27987:CDS 50.0%
CCTGTTGTGGCAATTCCCTT+TGG - chr4.4:11725697-11725716 None:intergenic 50.0%
CTTGAGGGAAATGAGGGAAG+TGG - chr4.4:11725665-11725684 None:intergenic 50.0%
GAAGGATCATCAGACAACCG+TGG + chr4.4:11725829-11725848 MS.gene27987:CDS 50.0%
TACAGAACCCAAGCTATGGC+TGG - chr4.4:11725880-11725899 None:intergenic 50.0%
TGAGGGAAATGAGGGAAGTG+GGG - chr4.4:11725663-11725682 None:intergenic 50.0%
TGCTGCAAAAGCTGCATCAC+AGG - chr4.4:11725788-11725807 None:intergenic 50.0%
! AGCTAGTGCTGTTCCTGCTA+TGG - chr4.4:11725740-11725759 None:intergenic 50.0%
! GGGTCAGCTTCTCTTGTTTG+TGG + chr4.4:11726071-11726090 MS.gene27987:CDS 50.0%
GCTGCAATTGCTGAAGCTGC+TGG + chr4.4:11726036-11726055 MS.gene27987:CDS 55.0%
!! GTGGTGGGTTAGCAGCTTCA+GGG + chr4.4:11725977-11725996 MS.gene27987:CDS 55.0%
!! GGTGGTGGGTTAGCAGCTTC+AGG + chr4.4:11725976-11725995 MS.gene27987:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 11725562 11726170 11725562 ID=MS.gene27987
chr4.4 mRNA 11725562 11726170 11725562 ID=MS.gene27987.t1;Parent=MS.gene27987
chr4.4 exon 11725562 11726170 11725562 ID=MS.gene27987.t1.exon1;Parent=MS.gene27987.t1
chr4.4 CDS 11725562 11726170 11725562 ID=cds.MS.gene27987.t1;Parent=MS.gene27987.t1
Gene Sequence

>MS.gene27987

ATGGCATCAACCATCATTGCAATGACCATGATCAACTCAAAGTCCTTGAACACTACTAGTTCACCAAAGTTCCACAACCCAAATAAGCTCATTACAAACCCCACTTCCCTCATTTCCCTCAAGAACCTTCTTCCAAAGGGAATTGCCACAACAGGAAACATTAAACTATCAACTTCCATAGCAGGAACAGCACTAGCTGGAGCAATATTCTCAACACTCTCAACCTGTGATGCAGCTTTTGCAGCACAACAAATAGCTGAAATAGCAGAAGGATCATCAGACAACCGTGGTTTAGCACTTCTGTTACCATTAATTCCAGCCATAGCTTGGGTTCTGTACAACATTCTTCAACCAGCACTTAACCAAATCAACCGTATGCGTAACGATAAAGGAGTTATCATTGGTTTAGGTCTTGGTGGTGGGTTAGCAGCTTCAGGGTTTGTGTCAACACAAGATGCAATGGCTAGTGAAATGGCTGCAATTGCTGAAGCTGCTGGTAGTGATAATAGGGGTCAGCTTCTCTTGTTTGTGGTAGCACCAGCTATTCTTTGGGTTCTTTATAATATTTTGCAGCCTGCTCTTAACCAAATCAACAGAATGAGACAGTGA

Protein sequence

>MS.gene27987.t1

MASTIIAMTMINSKSLNTTSSPKFHNPNKLITNPTSLISLKNLLPKGIATTGNIKLSTSIAGTALAGAIFSTLSTCDAAFAAQQIAEIAEGSSDNRGLALLLPLIPAIAWVLYNILQPALNQINRMRNDKGVIIGLGLGGGLAASGFVSTQDAMASEMAAIAEAAGSDNRGQLLLFVVAPAILWVLYNILQPALNQINRMRQ