AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36341


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36341.t1 MTR_2g101380 97.906 191 4 0 1 191 1 191 1.10e-139 387
MS.gene36341.t1 MTR_1g011510 53.846 104 46 2 72 174 60 162 1.95e-24 94.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36341.t1 AT3G16250 64.641 181 50 5 18 185 20 199 2.73e-77 230
MS.gene36341.t1 AT4G32590 53.846 104 46 2 72 174 70 172 1.57e-25 97.4
MS.gene36341.t1 AT4G32590 53.763 93 41 2 72 163 70 161 3.36e-19 80.9

Find 43 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTCTGAGTCCTTCCTTTC+AGG 0.071476 2.2:-2165392 None:intergenic
AGTGCCAACTGCTCTAATTT+TGG 0.198239 2.2:-2165366 None:intergenic
CATGCTAATCTCCAATTCTT+TGG 0.246574 2.2:-2166194 None:intergenic
GAGAGAGATGTGAATGCATT+AGG 0.312328 2.2:-2165293 None:intergenic
AGAGAGATGTGAATGCATTA+GGG 0.331294 2.2:-2165292 None:intergenic
TCTCTGAGTCCTTCCTTTCA+GGG 0.356821 2.2:-2165391 None:intergenic
TCTAATGACCCTCCCTCTAT+AGG 0.376560 2.2:+2165425 MS.gene36341:CDS
GACAAATGCAAAGCCTATAG+AGG 0.388630 2.2:-2165438 None:intergenic
GATTCTAATATTGAACTCTA+TGG 0.391241 2.2:+2165695 MS.gene36341:CDS
TAGGGGTTAGTTTGAGTTGG+AGG 0.393377 2.2:-2165274 None:intergenic
CTTGCAAAGAGTGTTGTGTT+TGG 0.404915 2.2:-2165341 None:intergenic
CATTCATGTCCTTTCCACTC+TGG 0.430702 2.2:-2166396 None:intergenic
TGCACATCTGGTGTTCCATC+TGG 0.437999 2.2:-2165626 None:intergenic
CATTAGGGGTTAGTTTGAGT+TGG 0.450485 2.2:-2165277 None:intergenic
AATTGCTATTAGGTTCTTGA+AGG 0.451453 2.2:+2165958 MS.gene36341:intron
GCTGTACGATAATGCACATC+TGG 0.480063 2.2:-2165638 None:intergenic
GTGGGTGAAGCAGATTCTAC+AGG 0.486617 2.2:+2166227 MS.gene36341:CDS
CTATTAGGTTCTTGAAGGCA+AGG 0.503895 2.2:+2165963 MS.gene36341:intron
GAAGCTTAGGAACATAATGT+TGG 0.511451 2.2:+2165673 MS.gene36341:CDS
CTTGTGGTGGACAGAAGCTT+AGG 0.512735 2.2:+2165660 MS.gene36341:CDS
ACATGAATGGAAATATGAAA+AGG 0.521149 2.2:+2166410 MS.gene36341:CDS
CAGAGTGGAAAGGACATGAA+TGG 0.529406 2.2:+2166397 MS.gene36341:CDS
AGAACCAAAATTAGAGCAGT+TGG 0.536986 2.2:+2165362 MS.gene36341:CDS
TGGCACTGTCCCTGAAAGGA+AGG 0.557748 2.2:+2165382 MS.gene36341:CDS
TGGAACTTGTGCTACATGTA+TGG 0.561485 2.2:+2165838 MS.gene36341:CDS
TAGTCTGTGTTGCTACCAGA+TGG 0.569681 2.2:+2165611 MS.gene36341:intron
TGGGTGAAGCAGATTCTACA+GGG 0.575014 2.2:+2166228 MS.gene36341:CDS
TCTTTCTCTTTGTCAGTGCG+AGG 0.589508 2.2:-2165997 None:intergenic
CAGTTGGCACTGTCCCTGAA+AGG 0.596043 2.2:+2165378 MS.gene36341:CDS
TTAGCATGTCAAACAACTGT+GGG 0.604116 2.2:+2166209 MS.gene36341:CDS
TTGTGCTACATGTATGGTAG+AGG 0.614537 2.2:+2165844 MS.gene36341:CDS
ACAAATGCAAAGCCTATAGA+GGG 0.622154 2.2:-2165437 None:intergenic
GTGCATTATCGTACAGCTTG+TGG 0.644594 2.2:+2165644 MS.gene36341:CDS
CAACAATTACCAGAGTGGAA+AGG 0.648231 2.2:+2166387 MS.gene36341:CDS
TGAAGCAGATTCTACAGGGG+TGG 0.651914 2.2:+2166232 MS.gene36341:CDS
AATGCAAAGCCTATAGAGGG+AGG 0.652647 2.2:-2165434 None:intergenic
ATGCAAAGCCTATAGAGGGA+GGG 0.653148 2.2:-2165433 None:intergenic
ATTAGCATGTCAAACAACTG+TGG 0.658456 2.2:+2166208 MS.gene36341:CDS
GAGAGATGTGAATGCATTAG+GGG 0.663309 2.2:-2165291 None:intergenic
TGATTCAACAATTACCAGAG+TGG 0.670303 2.2:+2166382 MS.gene36341:CDS
GAGTCAGCAGACTCTTTGTG+AGG 0.678932 2.2:+2166441 MS.gene36341:CDS
CATTATCGTACAGCTTGTGG+TGG 0.680953 2.2:+2165647 MS.gene36341:CDS
GGGTGAAGCAGATTCTACAG+GGG 0.770570 2.2:+2166229 MS.gene36341:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTTTAATTGCTATT+AGG + chr2.2:2165948-2165967 MS.gene36341:intron 10.0%
!!! TTTATTTTTAAGCAATAATT+GGG + chr2.2:2165756-2165775 MS.gene36341:intron 10.0%
!!! TTTTATTTTTAAGCAATAAT+TGG + chr2.2:2165755-2165774 MS.gene36341:intron 10.0%
!! CAGATTAGAAATTAATAAAA+TGG + chr2.2:2166285-2166304 MS.gene36341:intron 15.0%
!! ATTTATGTTGAGCAATATAT+TGG + chr2.2:2166330-2166349 MS.gene36341:intron 20.0%
!!! AATTAATGTCTAATGTTTTG+TGG + chr2.2:2165586-2165605 MS.gene36341:intron 20.0%
!!! AGCAAAAATACACATTTTAT+TGG + chr2.2:2165896-2165915 MS.gene36341:intron 20.0%
!!! GCAAAAATACACATTTTATT+GGG + chr2.2:2165897-2165916 MS.gene36341:intron 20.0%
!!! GTTAGTACTACTAATTTTTT+GGG + chr2.2:2166254-2166273 MS.gene36341:intron 20.0%
!!! TTTTATAGAAACCAAAGAAT+TGG + chr2.2:2166183-2166202 MS.gene36341:intron 20.0%
! AAGAGAAAGAAAAACTTAAG+AGG + chr2.2:2166010-2166029 MS.gene36341:CDS 25.0%
! AATTTAACAAAATTCTACCC+TGG - chr2.2:2165808-2165827 None:intergenic 25.0%
! ACATGAATGGAAATATGAAA+AGG + chr2.2:2166410-2166429 MS.gene36341:CDS 25.0%
! AGAGAAAGAAAAACTTAAGA+GGG + chr2.2:2166011-2166030 MS.gene36341:CDS 25.0%
! GATTCTAATATTGAACTCTA+TGG + chr2.2:2165695-2165714 MS.gene36341:CDS 25.0%
! GTTAGTGAGATAATTACATA+AGG - chr2.2:2165722-2165741 None:intergenic 25.0%
!! AATTTTGTTAAATTGTGGAG+GGG + chr2.2:2165814-2165833 MS.gene36341:CDS 25.0%
!! AGAATTTTGTTAAATTGTGG+AGG + chr2.2:2165812-2165831 MS.gene36341:CDS 25.0%
!! GAATTTTGTTAAATTGTGGA+GGG + chr2.2:2165813-2165832 MS.gene36341:CDS 25.0%
!! GGTAGAATTTTGTTAAATTG+TGG + chr2.2:2165809-2165828 MS.gene36341:CDS 25.0%
!!! ATTTTATTGTTGCTTTTTCC+AGG + chr2.2:2165787-2165806 MS.gene36341:intron 25.0%
!!! GGTTAGTACTACTAATTTTT+TGG + chr2.2:2166253-2166272 MS.gene36341:intron 25.0%
!!! TTTTATTGTTGCTTTTTCCA+GGG + chr2.2:2165788-2165807 MS.gene36341:intron 25.0%
! AATTGCTATTAGGTTCTTGA+AGG + chr2.2:2165958-2165977 MS.gene36341:intron 30.0%
! ATTTTCATTCCATGTGATGT+AGG + chr2.2:2166356-2166375 MS.gene36341:intron 30.0%
! ATTTTGTTAAATTGTGGAGG+GGG + chr2.2:2165815-2165834 MS.gene36341:CDS 30.0%
!!! TGGTCTGAATTTTTTATGTG+AGG - chr2.2:2165324-2165343 None:intergenic 30.0%
ACAAATGCAAAGCCTATAGA+GGG - chr2.2:2165440-2165459 None:intergenic 35.0%
AGAACCAAAATTAGAGCAGT+TGG + chr2.2:2165362-2165381 MS.gene36341:CDS 35.0%
AGAGAGATGTGAATGCATTA+GGG - chr2.2:2165295-2165314 None:intergenic 35.0%
ATGTGAAATGTGAACTCTTG+TGG + chr2.2:2166126-2166145 MS.gene36341:intron 35.0%
ATTAGCATGTCAAACAACTG+TGG + chr2.2:2166208-2166227 MS.gene36341:CDS 35.0%
CATGCTAATCTCCAATTCTT+TGG - chr2.2:2166197-2166216 None:intergenic 35.0%
GAAGCTTAGGAACATAATGT+TGG + chr2.2:2165673-2165692 MS.gene36341:CDS 35.0%
TGATTCAACAATTACCAGAG+TGG + chr2.2:2166382-2166401 MS.gene36341:CDS 35.0%
TTAGCATGTCAAACAACTGT+GGG + chr2.2:2166209-2166228 MS.gene36341:CDS 35.0%
CAACAATTACCAGAGTGGAA+AGG + chr2.2:2166387-2166406 MS.gene36341:CDS 40.0%
CTATTAGGTTCTTGAAGGCA+AGG + chr2.2:2165963-2165982 MS.gene36341:intron 40.0%
GAATCACAACCTACATCACA+TGG - chr2.2:2166368-2166387 None:intergenic 40.0%
GACAAATGCAAAGCCTATAG+AGG - chr2.2:2165441-2165460 None:intergenic 40.0%
GAGAGAGATGTGAATGCATT+AGG - chr2.2:2165296-2165315 None:intergenic 40.0%
GAGAGATGTGAATGCATTAG+GGG - chr2.2:2165294-2165313 None:intergenic 40.0%
TGGAACTTGTGCTACATGTA+TGG + chr2.2:2165838-2165857 MS.gene36341:CDS 40.0%
TTGTGCTACATGTATGGTAG+AGG + chr2.2:2165844-2165863 MS.gene36341:CDS 40.0%
! AGTGCCAACTGCTCTAATTT+TGG - chr2.2:2165369-2165388 None:intergenic 40.0%
!! CATTAGGGGTTAGTTTGAGT+TGG - chr2.2:2165280-2165299 None:intergenic 40.0%
!! CTTGCAAAGAGTGTTGTGTT+TGG - chr2.2:2165344-2165363 None:intergenic 40.0%
AATGCAAAGCCTATAGAGGG+AGG - chr2.2:2165437-2165456 None:intergenic 45.0%
ATGCAAAGCCTATAGAGGGA+GGG - chr2.2:2165436-2165455 None:intergenic 45.0%
CAGAGTGGAAAGGACATGAA+TGG + chr2.2:2166397-2166416 MS.gene36341:CDS 45.0%
CATTATCGTACAGCTTGTGG+TGG + chr2.2:2165647-2165666 MS.gene36341:CDS 45.0%
CATTCATGTCCTTTCCACTC+TGG - chr2.2:2166399-2166418 None:intergenic 45.0%
GCTGTACGATAATGCACATC+TGG - chr2.2:2165641-2165660 None:intergenic 45.0%
GTGCATTATCGTACAGCTTG+TGG + chr2.2:2165644-2165663 MS.gene36341:CDS 45.0%
TCTAATGACCCTCCCTCTAT+AGG + chr2.2:2165425-2165444 MS.gene36341:CDS 45.0%
TCTCTGAGTCCTTCCTTTCA+GGG - chr2.2:2165394-2165413 None:intergenic 45.0%
TCTTTCTCTTTGTCAGTGCG+AGG - chr2.2:2166000-2166019 None:intergenic 45.0%
TGGGTGAAGCAGATTCTACA+GGG + chr2.2:2166228-2166247 MS.gene36341:CDS 45.0%
TTGTTAAATTGTGGAGGGGG+TGG + chr2.2:2165818-2165837 MS.gene36341:CDS 45.0%
! TAGTCTGTGTTGCTACCAGA+TGG + chr2.2:2165611-2165630 MS.gene36341:intron 45.0%
!! TAGGGGTTAGTTTGAGTTGG+AGG - chr2.2:2165277-2165296 None:intergenic 45.0%
GGGTGAAGCAGATTCTACAG+GGG + chr2.2:2166229-2166248 MS.gene36341:CDS 50.0%
GTCTCTGAGTCCTTCCTTTC+AGG - chr2.2:2165395-2165414 None:intergenic 50.0%
GTGGGTGAAGCAGATTCTAC+AGG + chr2.2:2166227-2166246 MS.gene36341:CDS 50.0%
TGAAGCAGATTCTACAGGGG+TGG + chr2.2:2166232-2166251 MS.gene36341:CDS 50.0%
TGCACATCTGGTGTTCCATC+TGG - chr2.2:2165629-2165648 None:intergenic 50.0%
!! CTTGTGGTGGACAGAAGCTT+AGG + chr2.2:2165660-2165679 MS.gene36341:CDS 50.0%
CAGTTGGCACTGTCCCTGAA+AGG + chr2.2:2165378-2165397 MS.gene36341:CDS 55.0%
TGGCACTGTCCCTGAAAGGA+AGG + chr2.2:2165382-2165401 MS.gene36341:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 2165266 2166461 2165266 ID=MS.gene36341
chr2.2 mRNA 2165266 2166461 2165266 ID=MS.gene36341.t1;Parent=MS.gene36341
chr2.2 exon 2165266 2165463 2165266 ID=MS.gene36341.t1.exon1;Parent=MS.gene36341.t1
chr2.2 CDS 2165266 2165463 2165266 ID=cds.MS.gene36341.t1;Parent=MS.gene36341.t1
chr2.2 exon 2165614 2165724 2165614 ID=MS.gene36341.t1.exon2;Parent=MS.gene36341.t1
chr2.2 CDS 2165614 2165724 2165614 ID=cds.MS.gene36341.t1;Parent=MS.gene36341.t1
chr2.2 exon 2165809 2165865 2165809 ID=MS.gene36341.t1.exon3;Parent=MS.gene36341.t1
chr2.2 CDS 2165809 2165865 2165809 ID=cds.MS.gene36341.t1;Parent=MS.gene36341.t1
chr2.2 exon 2165970 2166032 2165970 ID=MS.gene36341.t1.exon4;Parent=MS.gene36341.t1
chr2.2 CDS 2165970 2166032 2165970 ID=cds.MS.gene36341.t1;Parent=MS.gene36341.t1
chr2.2 exon 2166191 2166253 2166191 ID=MS.gene36341.t1.exon5;Parent=MS.gene36341.t1
chr2.2 CDS 2166191 2166253 2166191 ID=cds.MS.gene36341.t1;Parent=MS.gene36341.t1
chr2.2 exon 2166378 2166461 2166378 ID=MS.gene36341.t1.exon6;Parent=MS.gene36341.t1
chr2.2 CDS 2166378 2166461 2166378 ID=cds.MS.gene36341.t1;Parent=MS.gene36341.t1
Gene Sequence

>MS.gene36341

ATGTCACTCCTCCAACTCAAACTAACCCCTAATGCATTCACATCTCTCTCATCTTCCTCACATAAAAAATTCAGACCAAACACAACACTCTTTGCAAGAACCAAAATTAGAGCAGTTGGCACTGTCCCTGAAAGGAAGGACTCAGAGACAACTACTGATTCTAATGACCCTCCCTCTATAGGCTTTGCATTTGTCAGTGTAAGTTCAAATTCTTAATACTATACTAAATGTACATACATGTAACATAACATAACATAACATGTAACTAATTAATTCTACTTATATATAGTTGATTTTACATTTTTTTATTAATTTTATGAAATTAATGTCTAATGTTTTGTGGATTAGTCTGTGTTGCTACCAGATGGAACACCAGATGTGCATTATCGTACAGCTTGTGGTGGACAGAAGCTTAGGAACATAATGTTGGATTCTAATATTGAACTCTATGGACCTTATGTAATTATCTCACTAACTTAAATTATTTTATTTTATTTTTAAGCAATAATTGGGTGATTATTATTTTATTGTTGCTTTTTCCAGGGTAGAATTTTGTTAAATTGTGGAGGGGGTGGAACTTGTGCTACATGTATGGTAGAGGTGAGCTATAATAATCTTATATAATTTTCTAGCAAAAATACACATTTTATTGGGTAGTATATAGTTATATAGTACTTATTGTATTTTTTTTTAATTGCTATTAGGTTCTTGAAGGCAAGGAGCTTCTGAGTCCTCGCACTGACAAAGAGAAAGAAAAACTTAAGAGGGTAAATGATTAAATCTTAACTTGATTAATGTCATTTTTTAATAAGAACTTAATTGTCATATTGAAAGAAAGAACAAACTTTTAGCATAAGTATATGTGAAATGTGAACTCTTGTGGTAAGAAAGATTACAATTATGATATCTTGTGTTTTTTTTATAGAAACCAAAGAATTGGAGATTAGCATGTCAAACAACTGTGGGTGAAGCAGATTCTACAGGGGTGGTTAGTACTACTAATTTTTTGGGTTAATATACAGATTAGAAATTAATAAAATGGCTGTATTTATTATTTATGCTTAATTTATGTTGAGCAATATATTGGTACATTTTCATTCCATGTGATGTAGGTTGTGATTCAACAATTACCAGAGTGGAAAGGACATGAATGGAAATATGAAAAGGATGATGATGAGTCAGCAGACTCTTTGTGA

Protein sequence

>MS.gene36341.t1

MSLLQLKLTPNAFTSLSSSSHKKFRPNTTLFARTKIRAVGTVPERKDSETTTDSNDPPSIGFAFVSSVLLPDGTPDVHYRTACGGQKLRNIMLDSNIELYGPYGRILLNCGGGGTCATCMVEVLEGKELLSPRTDKEKEKLKRKPKNWRLACQTTVGEADSTGVVVIQQLPEWKGHEWKYEKDDDESADSL