AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046149.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046149.01.T01 MTR_4g101750 75.113 221 55 0 2 222 553 773 2.84e-114 344
MsG0880046149.01.T01 MTR_4g101750 74.725 182 45 1 2 182 553 734 8.61e-89 277
MsG0880046149.01.T01 MTR_4g101740 59.195 174 35 2 2 175 98 235 3.79e-60 189
MsG0880046149.01.T01 MTR_7g044770 36.607 224 139 2 2 223 345 567 1.26e-44 157
MsG0880046149.01.T01 MTR_7g044770 36.607 224 139 2 2 223 522 744 3.45e-44 157
MsG0880046149.01.T01 MTR_2g020660 34.375 224 124 4 3 223 517 720 5.10e-36 134
MsG0880046149.01.T01 MTR_1g048000 26.633 199 126 4 27 214 619 808 7.12e-12 64.7
MsG0880046149.01.T01 MTR_2g069320 31.507 146 85 2 81 211 660 805 1.34e-11 63.9
MsG0880046149.01.T01 MTR_2g069310 35.000 100 64 1 116 214 709 808 4.79e-11 62.4
MsG0880046149.01.T01 MTR_2g069050 35.000 100 64 1 116 214 709 808 5.21e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046149.01.T01 AT1G62750 71.946 221 62 0 2 222 557 777 1.00e-110 335
MsG0880046149.01.T01 AT1G45332 39.732 224 132 2 2 223 345 567 6.65e-48 166
MsG0880046149.01.T01 AT1G45332 39.732 224 132 2 2 223 525 747 3.12e-47 166
MsG0880046149.01.T01 AT2G45030 39.732 224 132 2 2 223 525 747 3.41e-47 166
MsG0880046149.01.T01 AT3G12915 30.000 170 107 3 48 214 593 753 1.75e-11 63.5

Find 53 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAATTTCAACTTTCATTAT+AGG 0.216886 8:+69865217 None:intergenic
TGGTGTTCTAGCCGGCTTTC+CGG 0.222511 8:-69865371 MsG0880046149.01.T01:CDS
GTTACTCCTGAAGAACATTT+AGG 0.259112 8:-69865196 MsG0880046149.01.T01:CDS
ACATCTCCTAAATGTTCTTC+AGG 0.320044 8:+69865190 None:intergenic
CTTCCAGAAGAATTCATTAC+TGG 0.320246 8:-69865427 MsG0880046149.01.T01:CDS
GAACATTTAGGAGATGTAAT+TGG 0.332221 8:-69865184 MsG0880046149.01.T01:CDS
TGCTTTCCCTATAGTTTACT+TGG 0.338998 8:+69865590 None:intergenic
ACCCCAGTAATGAATTCTTC+TGG 0.342666 8:+69865424 None:intergenic
AAGCTTCTCTTGCTGCCAAC+TGG 0.389694 8:+69865281 None:intergenic
TCAAATTTGTATCCACTACT+TGG 0.404077 8:+69865472 None:intergenic
CTAGCCGGCTTTCCGGTTGT+TGG 0.421178 8:-69865364 MsG0880046149.01.T01:CDS
AGGTTCAAGCATTAACGGTT+TGG 0.428422 8:+69865237 None:intergenic
GTATCCACTACTTGGGTCCA+TGG 0.429904 8:+69865480 None:intergenic
GTCCATGGGTTCAAAATGTA+TGG 0.430865 8:+69865495 None:intergenic
ATTATAGGTTCAAGCATTAA+CGG 0.444891 8:+69865232 None:intergenic
TACTGAAACATCTCAGCAAT+AGG 0.458689 8:+69864847 None:intergenic
GCACGTTTGTCAATAATTTG+TGG 0.461924 8:+69864726 None:intergenic
CCTACTCAATGCAGTTTGAC+AGG 0.466205 8:-69864786 MsG0880046149.01.T01:CDS
GCTTTCCCTATAGTTTACTT+GGG 0.467404 8:+69865591 None:intergenic
TATGTGTTCAATAAACAATC+AGG 0.472517 8:-69865544 MsG0880046149.01.T01:CDS
TCAGTATGTCGGTACACTTA+GGG 0.480771 8:-69864830 MsG0880046149.01.T01:CDS
TATCCACTACTTGGGTCCAT+GGG 0.482451 8:+69865481 None:intergenic
ATACCTCACTTCTGTGACTT+CGG 0.483194 8:+69865564 None:intergenic
TTCAGTATGTCGGTACACTT+AGG 0.488588 8:-69864831 MsG0880046149.01.T01:CDS
TGACTTACCTTGAGTCCACC+TGG 0.492845 8:+69865115 None:intergenic
TACACCAACAACCGGAAAGC+CGG 0.518143 8:+69865360 None:intergenic
GCAGTTTGACAGGTTTGATA+CGG 0.520423 8:-69864776 MsG0880046149.01.T01:CDS
GGAGATGTAATTGGTGATCT+CGG 0.520638 8:-69865175 MsG0880046149.01.T01:CDS
CAAATTTGTATCCACTACTT+GGG 0.533919 8:+69865473 None:intergenic
ACTGCGCGTACACCAACAAC+CGG 0.534986 8:+69865352 None:intergenic
ATGAGCAATGGTGTTCTAGC+CGG 0.543808 8:-69865379 MsG0880046149.01.T01:CDS
GCTGAGATGTTTCAGTATGT+CGG 0.550369 8:-69864841 MsG0880046149.01.T01:CDS
TTCCAGAAGAATTCATTACT+GGG 0.551889 8:-69865426 MsG0880046149.01.T01:CDS
ATGCAGGTAGAAGCTAATGT+TGG 0.557514 8:-69865619 MsG0880046149.01.T01:CDS
TGGTGTACGCGCAGTACTTG+TGG 0.572358 8:-69865344 MsG0880046149.01.T01:CDS
CTTTCCCTATAGTTTACTTG+GGG 0.575513 8:+69865592 None:intergenic
GAACCCATGGACCCAAGTAG+TGG 0.580505 8:-69865484 MsG0880046149.01.T01:CDS
AAATGTGAGGGTATTCCAGT+TGG 0.582925 8:-69865296 MsG0880046149.01.T01:CDS
CCTGTCAAACTGCATTGAGT+AGG 0.586523 8:+69864786 None:intergenic
TGACAAACCAGGTGGACTCA+AGG 0.596794 8:-69865122 MsG0880046149.01.T01:intron
ACACTTAGGGTGCTGACAGA+GGG 0.607819 8:-69864817 MsG0880046149.01.T01:CDS
TACACTTAGGGTGCTGACAG+AGG 0.609312 8:-69864818 MsG0880046149.01.T01:CDS
GGATGGTAATTACCATGATG+TGG 0.612641 8:-69865323 MsG0880046149.01.T01:CDS
TCACATTTGAATCCACATCA+TGG 0.622810 8:+69865311 None:intergenic
TGGTTACATCTGCAAATTGA+CGG 0.633657 8:+69865515 None:intergenic
TCCAGAAGAATTCATTACTG+GGG 0.636103 8:-69865425 MsG0880046149.01.T01:CDS
GTGTTCAATAAACAATCAGG+TGG 0.650549 8:-69865541 MsG0880046149.01.T01:CDS
TTGGAAGAGTGCATGAGCAA+TGG 0.663387 8:-69865391 MsG0880046149.01.T01:CDS
TGATGTGGATTCAAATGTGA+GGG 0.666011 8:-69865308 MsG0880046149.01.T01:CDS
ACTGCATTGAGTAGGATGCA+CGG 0.668859 8:+69864794 None:intergenic
TTTCCGAAGTCACAGAAGTG+AGG 0.671983 8:-69865567 MsG0880046149.01.T01:CDS
GTACGCGCAGTACTTGTGGA+TGG 0.703304 8:-69865340 MsG0880046149.01.T01:CDS
ATGATGTGGATTCAAATGTG+AGG 0.710458 8:-69865309 MsG0880046149.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCGATAATAATTTGAAAAA+TGG + Chr8:69865392-69865411 None:intergenic 15.0%
!! TCAAATTTAATAAATGAACA+TGG + Chr8:69865279-69865298 None:intergenic 15.0%
!! TGTTCATTTATTAAATTTGA+AGG - Chr8:69865279-69865298 MsG0880046149.01.T01:CDS 15.0%
!! ACAATTTCAACTTTCATTAT+AGG + Chr8:69865140-69865159 None:intergenic 20.0%
!! ATATATATTAATGACGTATC+TGG - Chr8:69865334-69865353 MsG0880046149.01.T01:CDS 20.0%
!! TGTCACAAAAACTATTTATT+TGG + Chr8:69865217-69865236 None:intergenic 20.0%
! ATTATAGGTTCAAGCATTAA+CGG + Chr8:69865125-69865144 None:intergenic 25.0%
! TATGTGTTCAATAAACAATC+AGG - Chr8:69864810-69864829 MsG0880046149.01.T01:CDS 25.0%
! TTGAATGTAACTACAAGTAT+TGG + Chr8:69865363-69865382 None:intergenic 25.0%
!! GAATATTGTTTTCTTCCTTT+TGG - Chr8:69865451-69865470 MsG0880046149.01.T01:CDS 25.0%
CAAATTTGTATCCACTACTT+GGG + Chr8:69864884-69864903 None:intergenic 30.0%
GAACATTTAGGAGATGTAAT+TGG - Chr8:69865170-69865189 MsG0880046149.01.T01:CDS 30.0%
TCAAATTTGTATCCACTACT+TGG + Chr8:69864885-69864904 None:intergenic 30.0%
TTCCAGAAGAATTCATTACT+GGG - Chr8:69864928-69864947 MsG0880046149.01.T01:intron 30.0%
! AATAGTTTTTGTGACAAACC+AGG - Chr8:69865221-69865240 MsG0880046149.01.T01:CDS 30.0%
! TATTGTTTTCTTCCTTTTGG+TGG - Chr8:69865454-69865473 MsG0880046149.01.T01:CDS 30.0%
! TTCATTACTGGGGTTTTTAA+AGG - Chr8:69864939-69864958 MsG0880046149.01.T01:intron 30.0%
! TTTTCCCCAAGTAAACTATA+GGG - Chr8:69864758-69864777 MsG0880046149.01.T01:CDS 30.0%
ACATCTCCTAAATGTTCTTC+AGG + Chr8:69865167-69865186 None:intergenic 35.0%
ATGATGTGGATTCAAATGTG+AGG - Chr8:69865045-69865064 MsG0880046149.01.T01:intron 35.0%
CTTCCAGAAGAATTCATTAC+TGG - Chr8:69864927-69864946 MsG0880046149.01.T01:intron 35.0%
CTTTCCCTATAGTTTACTTG+GGG + Chr8:69864765-69864784 None:intergenic 35.0%
GCTTTCCCTATAGTTTACTT+GGG + Chr8:69864766-69864785 None:intergenic 35.0%
GTGTTCAATAAACAATCAGG+TGG - Chr8:69864813-69864832 MsG0880046149.01.T01:CDS 35.0%
GTTACTCCTGAAGAACATTT+AGG - Chr8:69865158-69865177 MsG0880046149.01.T01:CDS 35.0%
TACTGAAACATCTCAGCAAT+AGG + Chr8:69865510-69865529 None:intergenic 35.0%
TCACATTTGAATCCACATCA+TGG + Chr8:69865046-69865065 None:intergenic 35.0%
TCCAGAAGAATTCATTACTG+GGG - Chr8:69864929-69864948 MsG0880046149.01.T01:intron 35.0%
TGATGTGGATTCAAATGTGA+GGG - Chr8:69865046-69865065 MsG0880046149.01.T01:intron 35.0%
TGCTTTCCCTATAGTTTACT+TGG + Chr8:69864767-69864786 None:intergenic 35.0%
TGGTTACATCTGCAAATTGA+CGG + Chr8:69864842-69864861 None:intergenic 35.0%
! AACCATACATTTTGAACCCA+TGG - Chr8:69864857-69864876 MsG0880046149.01.T01:CDS 35.0%
! GTTTTCCCCAAGTAAACTAT+AGG - Chr8:69864757-69864776 MsG0880046149.01.T01:CDS 35.0%
!! TACTGGGGTTTTTAAAGGAT+TGG - Chr8:69864944-69864963 MsG0880046149.01.T01:intron 35.0%
ACCCCAGTAATGAATTCTTC+TGG + Chr8:69864933-69864952 None:intergenic 40.0%
AGGTTCAAGCATTAACGGTT+TGG + Chr8:69865120-69865139 None:intergenic 40.0%
ATACCTCACTTCTGTGACTT+CGG + Chr8:69864793-69864812 None:intergenic 40.0%
ATGCAGGTAGAAGCTAATGT+TGG - Chr8:69864735-69864754 MsG0880046149.01.T01:CDS 40.0%
GCAGTTTGACAGGTTTGATA+CGG - Chr8:69865578-69865597 MsG0880046149.01.T01:CDS 40.0%
GCTGAGATGTTTCAGTATGT+CGG - Chr8:69865513-69865532 MsG0880046149.01.T01:CDS 40.0%
GGATGGTAATTACCATGATG+TGG - Chr8:69865031-69865050 MsG0880046149.01.T01:intron 40.0%
GTCCATGGGTTCAAAATGTA+TGG + Chr8:69864862-69864881 None:intergenic 40.0%
TCAGTATGTCGGTACACTTA+GGG - Chr8:69865524-69865543 MsG0880046149.01.T01:CDS 40.0%
TTCAGTATGTCGGTACACTT+AGG - Chr8:69865523-69865542 MsG0880046149.01.T01:CDS 40.0%
! AGTTTTTGTGACAAACCAGG+TGG - Chr8:69865224-69865243 MsG0880046149.01.T01:CDS 40.0%
! CTGTTTTCACAACCACCAAA+AGG + Chr8:69865469-69865488 None:intergenic 40.0%
! GGAGATGTAATTGGTGATCT+CGG - Chr8:69865179-69865198 MsG0880046149.01.T01:CDS 40.0%
!! AAATGTGAGGGTATTCCAGT+TGG - Chr8:69865058-69865077 MsG0880046149.01.T01:intron 40.0%
!! CTTTTGGTGGTTGTGAAAAC+AGG - Chr8:69865467-69865486 MsG0880046149.01.T01:CDS 40.0%
ACTGCATTGAGTAGGATGCA+CGG + Chr8:69865563-69865582 None:intergenic 45.0%
ATGAGCAATGGTGTTCTAGC+CGG - Chr8:69864975-69864994 MsG0880046149.01.T01:intron 45.0%
CCTACTCAATGCAGTTTGAC+AGG - Chr8:69865568-69865587 MsG0880046149.01.T01:CDS 45.0%
CCTGTCAAACTGCATTGAGT+AGG + Chr8:69865571-69865590 None:intergenic 45.0%
TATCCACTACTTGGGTCCAT+GGG + Chr8:69864876-69864895 None:intergenic 45.0%
TTTCCGAAGTCACAGAAGTG+AGG - Chr8:69864787-69864806 MsG0880046149.01.T01:CDS 45.0%
! GGCAGCAAGAGAAGCTTTTA+GGG - Chr8:69865079-69865098 MsG0880046149.01.T01:intron 45.0%
! TGGCAGCAAGAGAAGCTTTT+AGG - Chr8:69865078-69865097 MsG0880046149.01.T01:intron 45.0%
! TTGGAAGAGTGCATGAGCAA+TGG - Chr8:69864963-69864982 MsG0880046149.01.T01:intron 45.0%
! TTGGTGGTTGTGAAAACAGG+TGG - Chr8:69865470-69865489 MsG0880046149.01.T01:CDS 45.0%
!! GCAAGAGAAGCTTTTAGGGA+AGG - Chr8:69865083-69865102 MsG0880046149.01.T01:intron 45.0%
AAGCTTCTCTTGCTGCCAAC+TGG + Chr8:69865076-69865095 None:intergenic 50.0%
GGTGATCTCGGCAGAAAAAG+AGG - Chr8:69865191-69865210 MsG0880046149.01.T01:CDS 50.0%
GTATCCACTACTTGGGTCCA+TGG + Chr8:69864877-69864896 None:intergenic 50.0%
TACACCAACAACCGGAAAGC+CGG + Chr8:69864997-69865016 None:intergenic 50.0%
TGACTTACCTTGAGTCCACC+TGG + Chr8:69865242-69865261 None:intergenic 50.0%
! TGACAAACCAGGTGGACTCA+AGG - Chr8:69865232-69865251 MsG0880046149.01.T01:CDS 50.0%
!! ACACTTAGGGTGCTGACAGA+GGG - Chr8:69865537-69865556 MsG0880046149.01.T01:CDS 50.0%
!! TACACTTAGGGTGCTGACAG+AGG - Chr8:69865536-69865555 MsG0880046149.01.T01:CDS 50.0%
ACTGCGCGTACACCAACAAC+CGG + Chr8:69865005-69865024 None:intergenic 55.0%
GAACCCATGGACCCAAGTAG+TGG - Chr8:69864870-69864889 MsG0880046149.01.T01:intron 55.0%
GTACGCGCAGTACTTGTGGA+TGG - Chr8:69865014-69865033 MsG0880046149.01.T01:intron 55.0%
TGGTGTACGCGCAGTACTTG+TGG - Chr8:69865010-69865029 MsG0880046149.01.T01:intron 55.0%
! TGGTGTTCTAGCCGGCTTTC+CGG - Chr8:69864983-69865002 MsG0880046149.01.T01:intron 55.0%
! CTAGCCGGCTTTCCGGTTGT+TGG - Chr8:69864990-69865009 MsG0880046149.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 69864735 69865641 69864735 ID=MsG0880046149.01;Name=MsG0880046149.01
Chr8 mRNA 69864735 69865641 69864735 ID=MsG0880046149.01.T01;Parent=MsG0880046149.01;Name=MsG0880046149.01.T01;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|1|1|2|0|223
Chr8 exon 69865123 69865641 69865123 ID=MsG0880046149.01.T01:exon:27327;Parent=MsG0880046149.01.T01
Chr8 exon 69864735 69864887 69864735 ID=MsG0880046149.01.T01:exon:27326;Parent=MsG0880046149.01.T01
Chr8 CDS 69865123 69865641 69865123 ID=MsG0880046149.01.T01:cds;Parent=MsG0880046149.01.T01
Chr8 CDS 69864735 69864887 69864735 ID=MsG0880046149.01.T01:cds;Parent=MsG0880046149.01.T01
Gene Sequence

>MsG0880046149.01.T01

ATGCAGGTAGAAGCTAATGTTGGTTTTCCCCAAGTAAACTATAGGGAAAGCATTTCCGAAGTCACAGAAGTGAGGTATGTGTTCAATAAACAATCAGGTGGACACCGTCAATTTGCAGATGTAACCATACATTTTGAACCCATGGACCCAAGTAGTGGATACAAATTTGAGAATAAAATCAAAGAAGAAGCACTTCCAGAAGAATTCATTACTGGGGTTTTTAAAGGATTGGAAGAGTGCATGAGCAATGGTGTTCTAGCCGGCTTTCCGGTTGTTGGTGTACGCGCAGTACTTGTGGATGGTAATTACCATGATGTGGATTCAAATGTGAGGGTATTCCAGTTGGCAGCAAGAGAAGCTTTTAGGGAAGGAATGAGAAAAGCCAAACCGTTAATGCTTGAACCTATAATGAAAGTTGAAATTGTTACTCCTGAAGAACATTTAGGAGATGTAATTGGTGATCTCGGCAGAAAAAGAGGCCAAATAAATAGTTTTTGTGACAAACCAGGTGGACTCAAGGTGGTTGATTCATTAGTACCTATTGCTGAGATGTTTCAGTATGTCGGTACACTTAGGGTGCTGACAGAGGGCCGTGCATCCTACTCAATGCAGTTTGACAGGTTTGATACGGTATCTCGAAACATTCAGAATGAACTTACCACAAATTATTGA

Protein sequence

>MsG0880046149.01.T01

MQVEANVGFPQVNYRESISEVTEVRYVFNKQSGGHRQFADVTIHFEPMDPSSGYKFENKIKEEALPEEFITGVFKGLEECMSNGVLAGFPVVGVRAVLVDGNYHDVDSNVRVFQLAAREAFREGMRKAKPLMLEPIMKVEIVTPEEHLGDVIGDLGRKRGQINSFCDKPGGLKVVDSLVPIAEMFQYVGTLRVLTEGRASYSMQFDRFDTVSRNIQNELTTNY*