AlfalfaGEDB | Msa0133280

M. sativa cultivar zhongmu-4 / Msa0133280

KEGG: K01534
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0133280	XP_024642361.1	96.691	272	9	0	1	272	1	272	0.0	538

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0133280	A0A396JW40	96.691	272	9	0	1	272	1	272	0.0	538

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000880	Msa0133280	0.825913	3.616413e-54	-8.615850e-47
Msa0001940	Msa0133280	0.840160	1.027572e-57	-8.615850e-47
Msa0002710	Msa0133280	0.804674	1.929075e-49	-8.615850e-47
Msa0005890	Msa0133280	0.828249	9.986056e-55	-8.615850e-47
Msa0017950	Msa0133280	0.842993	1.841669e-58	-8.615850e-47
Msa0041850	Msa0133280	0.802885	4.536003e-49	-8.615850e-47
Msa0048510	Msa0133280	0.810712	1.005662e-50	-8.615850e-47
Msa0054690	Msa0133280	0.816126	6.494805e-52	-8.615850e-47
Msa0077340	Msa0133280	0.849535	3.039061e-60	-8.615850e-47
Msa0077560	Msa0133280	0.835844	1.324675e-56	-8.615850e-47
Msa0081390	Msa0133280	0.803798	2.935118e-49	-8.615850e-47
Msa0081450	Msa0133280	0.803470	3.433301e-49	-8.615850e-47
Msa0081520	Msa0133280	0.803415	3.524764e-49	-8.615850e-47
Msa0089980	Msa0133280	0.829394	5.274924e-55	-8.615850e-47
Msa0090010	Msa0133280	0.828714	7.709640e-55	-8.615850e-47
Msa0093850	Msa0133280	0.810140	1.336366e-50	-8.615850e-47
Msa0094780	Msa0133280	0.850936	1.229600e-60	-8.615850e-47
Msa0097770	Msa0133280	0.802203	6.270382e-49	-8.615850e-47
Msa0106620	Msa0133280	0.821439	4.036377e-53	-8.615850e-47
Msa0106710	Msa0133280	0.819016	1.449299e-52	-8.615850e-47
Msa0125360	Msa0133280	0.841712	4.024287e-58	-8.615850e-47
Msa0133150	Msa0133280	0.963689	2.152012e-122	-8.615850e-47
Msa0133280	Msa0173690	0.816069	6.687162e-52	-8.615850e-47
Msa0133280	Msa0173890	0.800487	1.408258e-48	-8.615850e-47
Msa0133280	Msa0174450	0.817594	3.040749e-52	-8.615850e-47
Msa0133280	Msa0175930	0.819899	9.115634e-53	-8.615850e-47
Msa0133280	Msa0179130	0.802957	4.384253e-49	-8.615850e-47
Msa0133280	Msa0181650	0.826296	2.932731e-54	-8.615850e-47
Msa0133280	Msa0190110	0.809150	2.181347e-50	-8.615850e-47
Msa0133280	Msa0190570	0.822558	2.221230e-53	-8.615850e-47
Msa0133280	Msa0221000	0.859131	5.113338e-63	-8.615850e-47
Msa0133280	Msa0223080	0.804094	2.547897e-49	-8.615850e-47
Msa0133280	Msa0225100	0.814380	1.586624e-51	-8.615850e-47
Msa0133280	Msa0225220	0.803182	3.939258e-49	-8.615850e-47
Msa0133280	Msa0225480	0.831571	1.548206e-55	-8.615850e-47
Msa0133280	Msa0225860	0.843356	1.473486e-58	-8.615850e-47
Msa0133280	Msa0237210	0.820060	8.372821e-53	-8.615850e-47
Msa0133280	Msa0242590	0.800632	1.316012e-48	-8.615850e-47
Msa0133280	Msa0247940	0.807958	3.918781e-50	-8.615850e-47
Msa0133280	Msa0260510	0.810361	1.197731e-50	-8.615850e-47
Msa0133280	Msa0266180	0.802976	4.344720e-49	-8.615850e-47
Msa0133280	Msa0268000	0.829256	5.698066e-55	-8.615850e-47
Msa0133280	Msa0271880	0.842232	2.932212e-58	-8.615850e-47
Msa0133280	Msa0274980	0.821649	3.608754e-53	-8.615850e-47
Msa0133280	Msa0279690	0.819777	9.720147e-53	-8.615850e-47
Msa0133280	Msa0281470	0.804358	2.244919e-49	-8.615850e-47
Msa0133280	Msa0287740	0.817875	2.627659e-52	-8.615850e-47
Msa0133280	Msa0298470	0.858332	8.857418e-63	-8.615850e-47
Msa0133280	Msa0300270	0.842941	1.900662e-58	-8.615850e-47
Msa0133280	Msa0300320	0.851294	9.748251e-61	-8.615850e-47
Msa0133280	Msa0307270	0.861256	1.166006e-63	-8.615850e-47
Msa0133280	Msa0310850	0.844561	7.006567e-59	-8.615850e-47
Msa0133280	Msa0315690	0.833156	6.269642e-56	-8.615850e-47
Msa0133280	Msa0321620	0.813532	2.440261e-51	-8.615850e-47
Msa0133280	Msa0330380	0.817947	2.531922e-52	-8.615850e-47
Msa0133280	Msa0337700	0.853836	1.836081e-61	-8.615850e-47
Msa0133280	Msa0339490	0.803643	3.160901e-49	-8.615850e-47
Msa0133280	Msa0340500	0.856284	3.570256e-62	-8.615850e-47
Msa0133280	Msa0341690	0.832742	7.946872e-56	-8.615850e-47
Msa0133280	Msa0342010	0.810275	1.249808e-50	-8.615850e-47
Msa0133280	Msa0361910	0.854828	9.487221e-62	-8.615850e-47
Msa0133280	Msa0371030	0.805454	1.324290e-49	-8.615850e-47
Msa0133280	Msa0378720	0.833130	6.364215e-56	-8.615850e-47
Msa0133280	Msa0380620	0.801241	9.880717e-49	-8.615850e-47
Msa0133280	Msa0409830	0.826815	2.205051e-54	-8.615850e-47
Msa0133280	Msa0428370	0.823942	1.055236e-53	-8.615850e-47
Msa0133280	Msa0428580	0.814300	1.652476e-51	-8.615850e-47
Msa0133280	Msa0428920	0.806208	9.198160e-50	-8.615850e-47
Msa0133280	Msa0432010	0.800782	1.226087e-48	-8.615850e-47
Msa0133280	Msa0474730	0.834870	2.334597e-56	-8.615850e-47
Msa0133280	Msa0481890	0.833552	4.996153e-56	-8.615850e-47
Msa0133280	Msa0483100	0.853783	1.901601e-61	-8.615850e-47
Msa0133280	Msa0529030	0.849692	2.746359e-60	-8.615850e-47
Msa0133280	Msa0534720	0.837935	3.875021e-57	-8.615850e-47
Msa0133280	Msa0571290	0.808469	3.050676e-50	-8.615850e-47
Msa0133280	Msa0573850	0.828610	8.170773e-55	-8.615850e-47
Msa0133280	Msa0574390	0.804205	2.415273e-49	-8.615850e-47
Msa0133280	Msa0580370	0.867786	1.058304e-65	-8.615850e-47
Msa0133280	Msa0581470	0.847863	8.833913e-60	-8.615850e-47
Msa0133280	Msa0582480	0.817114	3.900586e-52	-8.615850e-47
Msa0133280	Msa0583360	-0.806870	6.666885e-50	-8.615850e-47
Msa0133280	Msa0586220	0.809112	2.222772e-50	-8.615850e-47
Msa0133280	Msa0590830	0.829039	6.432946e-55	-8.615850e-47
Msa0133280	Msa0597790	0.816885	4.390934e-52	-8.615850e-47
Msa0133280	Msa0611210	0.833520	5.087302e-56	-8.615850e-47
Msa0133280	Msa0615420	0.803077	4.140566e-49	-8.615850e-47
Msa0133280	Msa0624420	0.808299	3.315834e-50	-8.615850e-47
Msa0133280	Msa0624900	0.860916	1.479419e-63	-8.615850e-47
Msa0133280	Msa0626450	0.817755	2.797237e-52	-8.615850e-47
Msa0133280	Msa0626460	0.805975	1.029552e-49	-8.615850e-47
Msa0133280	Msa0626490	0.814862	1.241180e-51	-8.615850e-47
Msa0133280	Msa0651550	0.807163	5.780415e-50	-8.615850e-47
Msa0133280	Msa0652000	0.823317	1.478567e-53	-8.615850e-47
Msa0133280	Msa0658590	0.812952	3.271663e-51	-8.615850e-47
Msa0133280	Msa0658640	0.812562	3.983492e-51	-8.615850e-47
Msa0133280	Msa0660370	0.801513	8.692618e-49	-8.615850e-47
Msa0133280	Msa0661150	0.803919	2.770394e-49	-8.615850e-47
Msa0133280	Msa0664090	0.814542	1.461190e-51	-8.615850e-47
Msa0133280	Msa0664540	0.803335	3.661890e-49	-8.615850e-47
Msa0133280	Msa0664600	0.816281	5.994755e-52	-8.615850e-47
Msa0133280	Msa0666700	0.801098	1.056799e-48	-8.615850e-47
Msa0133280	Msa0674480	0.802154	6.419820e-49	-8.615850e-47
Msa0133280	Msa0697160	0.803626	3.187007e-49	-8.615850e-47
Msa0133280	Msa0698640	0.811840	5.725784e-51	-8.615850e-47
Msa0133280	Msa0709440	0.835597	1.529478e-56	-8.615850e-47
Msa0133280	Msa0721080	0.854381	1.278278e-61	-8.615850e-47
Msa0133280	Msa0735780	0.836066	1.163648e-56	-8.615850e-47
Msa0133280	Msa0741220	0.812326	4.485873e-51	-8.615850e-47
Msa0133280	Msa0761670	0.836011	1.201639e-56	-8.615850e-47
Msa0133280	Msa0795870	0.870817	1.095220e-66	-8.615850e-47
Msa0133280	Msa0824450	0.813504	2.474940e-51	-8.615850e-47
Msa0133280	Msa0833530	0.822000	2.994019e-53	-8.615850e-47
Msa0133280	Msa0833650	0.868367	6.880458e-66	-8.615850e-47
Msa0133280	Msa0878280	0.802785	4.758554e-49	-8.615850e-47
Msa0133280	Msa0894400	0.805097	1.573373e-49	-8.615850e-47
Msa0133280	Msa0902510	0.816006	6.907136e-52	-8.615850e-47
Msa0133280	Msa0941190	0.820356	7.164044e-53	-8.615850e-47
Msa0133280	Msa0967870	0.810757	9.833762e-51	-8.615850e-47
Msa0133280	Msa0979180	0.800053	1.726311e-48	-8.615850e-47
Msa0133280	Msa1028280	0.811377	7.218035e-51	-8.615850e-47
Msa0133280	Msa1034860	0.809008	2.339094e-50	-8.615850e-47
Msa0133280	Msa1036280	0.807166	5.771023e-50	-8.615850e-47
Msa0133280	Msa1037540	0.805132	1.547019e-49	-8.615850e-47
Msa0133280	Msa1044470	0.803811	2.917437e-49	-8.615850e-47
Msa0133280	Msa1072940	0.851132	1.082770e-60	-8.615850e-47
Msa0133280	Msa1081390	0.822532	2.252288e-53	-8.615850e-47
Msa0133280	Msa1084530	0.825225	5.264687e-54	-8.615850e-47
Msa0133280	Msa1103990	0.823872	1.096116e-53	-8.615850e-47
Msa0133280	Msa1111380	0.804764	1.847272e-49	-8.615850e-47
Msa0133280	Msa1116220	0.806789	6.936441e-50	-8.615850e-47
Msa0133280	Msa1125820	0.829006	6.552136e-55	-8.615850e-47
Msa0133280	Msa1125990	0.831418	1.688062e-55	-8.615850e-47
Msa0133280	Msa1132840	0.872237	3.711353e-67	-8.615850e-47
Msa0133280	Msa1139890	0.800899	1.160812e-48	-8.615850e-47
Msa0133280	Msa1145810	0.833951	3.970512e-56	-8.615850e-47
Msa0133280	Msa1155170	-0.813126	2.996935e-51	-8.615850e-47
Msa0133280	Msa1160920	0.854708	1.028095e-61	-8.615850e-47
Msa0133280	Msa1171290	0.840185	1.012726e-57	-8.615850e-47
Msa0133280	Msa1184120	0.818225	2.190919e-52	-8.615850e-47
Msa0133280	Msa1186020	0.849561	2.988525e-60	-8.615850e-47
Msa0133280	Msa1192570	0.829133	6.104699e-55	-8.615850e-47
Msa0133280	Msa1200890	0.849908	2.390066e-60	-8.615850e-47
Msa0133280	Msa1208430	0.804604	1.994252e-49	-8.615850e-47
Msa0133280	Msa1208950	0.842407	2.635640e-58	-8.615850e-47
Msa0133280	Msa1215870	0.810660	1.032037e-50	-8.615850e-47
Msa0133280	Msa1225860	-0.810566	1.081382e-50	-8.615850e-47
Msa0133280	Msa1226470	0.811759	5.963168e-51	-8.615850e-47
Msa0133280	Msa1226520	0.811272	7.605238e-51	-8.615850e-47
Msa0133280	Msa1228200	0.805298	1.428503e-49	-8.615850e-47
Msa0133280	Msa1228870	0.801939	7.106805e-49	-8.615850e-47
Msa0133280	Msa1240260	0.807238	5.573152e-50	-8.615850e-47
Msa0133280	Msa1253550	0.804923	1.710664e-49	-8.615850e-47
Msa0133280	Msa1258340	0.809839	1.551703e-50	-8.615850e-47
Msa0133280	Msa1276660	0.806058	9.888861e-50	-8.615850e-47
Msa0133280	Msa1277220	0.806912	6.531966e-50	-8.615850e-47
Msa0133280	Msa1292590	0.811673	6.223194e-51	-8.615850e-47
Msa0133280	Msa1298370	0.813659	2.289112e-51	-8.615850e-47
Msa0133280	Msa1307630	0.826983	2.010373e-54	-8.615850e-47
Msa0133280	Msa1320670	0.850451	1.683777e-60	-8.615850e-47
Msa0133280	Msa1325410	-0.807932	3.969105e-50	-8.615850e-47
Msa0133280	Msa1329980	0.813936	1.988699e-51	-8.615850e-47
Msa0133280	Msa1340200	0.805005	1.644730e-49	-8.615850e-47
Msa0133280	Msa1341840	0.821561	3.782254e-53	-8.615850e-47
Msa0133280	Msa1343170	0.811112	8.239220e-51	-8.615850e-47
Msa0133280	Msa1343180	0.819160	1.344001e-52	-8.615850e-47
Msa0133280	Msa1348090	0.815224	1.031654e-51	-8.615850e-47
Msa0133280	Msa1359690	0.818845	1.584333e-52	-8.615850e-47
Msa0133280	Msa1368750	0.802213	6.241483e-49	-8.615850e-47
Msa0133280	Msa1372040	0.818878	1.557546e-52	-8.615850e-47
Msa0133280	Msa1373100	0.828891	6.986650e-55	-8.615850e-47
Msa0133280	Msa1392580	0.848775	4.943075e-60	-8.615850e-47
Msa0133280	Msa1393820	0.811693	6.163031e-51	-8.615850e-47
Msa0133280	Msa1397400	0.817692	2.890483e-52	-8.615850e-47
Msa0133280	Msa1405460	0.849106	4.001496e-60	-8.615850e-47
Msa0133280	Msa1420950	0.807485	4.939769e-50	-8.615850e-47
Msa0133280	Msa1422950	0.829004	6.558441e-55	-8.615850e-47
Msa0133280	Msa1425880	0.805798	1.121703e-49	-8.615850e-47
Msa0133280	Msa1433810	0.802176	6.351018e-49	-8.615850e-47
Msa0133280	Msa1437930	0.801221	9.973912e-49	-8.615850e-47
Msa0133280	Msa1440310	0.809232	2.094837e-50	-8.615850e-47
Msa0133280	Msa1440880	0.837337	5.516756e-57	-8.615850e-47
Msa0133280	Msa1440960	0.812467	4.178686e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0133280	MtrunA17_Chr1g0202751	96.691	272	9	0	1	272	1	272	0.0	538

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 67 sgRNAs with CRISPR-Local

Find 286 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGCACTCATTCACATAATT+TGG	0.078016	1_3:+64541853	None:intergenic
GATAATATTGCTTAGAATAT+TGG	0.195303	1_3:-64542001	Msa0133280:three_prime_UTR
TCAACAATATTATCAGTTAA+AGG	0.201695	1_3:+64541895	None:intergenic
TAACATGATCAATGACATTT+TGG	0.223671	1_3:+64543209	None:intergenic
TCAGTCTGATGATGCATTCT+TGG	0.243516	1_3:-64542341	Msa0133280:intron
CAATTATTACTGTATTCTTT+TGG	0.246495	1_3:-64542087	Msa0133280:three_prime_UTR
ATTGAAATCCTTGAAAATTC+TGG	0.276980	1_3:+64542238	None:intergenic
AACAATAGTGGGGTTGTTCT+TGG	0.286826	1_3:+64542909	None:intergenic
CCTATGATTGCAATTATTGC+TGG	0.293251	1_3:-64541800	Msa0133280:three_prime_UTR
TCTGGACTCTTGCAGCATAC+TGG	0.312031	1_3:+64542256	None:intergenic
AATGTCATTGATCATGTTAA+TGG	0.315572	1_3:-64543205	Msa0133280:intron
TAGCTACTTTAACCGCAAAA+TGG	0.345953	1_3:-64543260	Msa0133280:exon
TGTGTACTTCATATTGTTGC+AGG	0.348192	1_3:+64542142	None:intergenic
GCAGATGGAGACGGCATATC+AGG	0.349775	1_3:+64542292	None:intergenic
TTGCAGGAAAGATGAAATCT+AGG	0.354095	1_3:-64543062	Msa0133280:CDS
TTTCCATCTACTATATCAAT+AGG	0.355572	1_3:+64542994	None:intergenic
TTCTTGGACTTGAGTAGATT+TGG	0.362992	1_3:+64542925	None:intergenic
CGCTCTTCGGCGATTGCATC+AGG	0.383202	1_3:+64542688	None:intergenic
GGCATATCAGGCAACTCTTC+AGG	0.388449	1_3:+64542304	None:intergenic
GTGAAATTGCAATCGAAAGC+TGG	0.396566	1_3:+64542205	None:intergenic
AGCACTCATTCACATAATTT+GGG	0.398276	1_3:+64541854	None:intergenic
ATCCTTATGTAGAACAATAG+TGG	0.407861	1_3:+64542897	None:intergenic
CTGGACTCTTGCAGCATACT+GGG	0.413396	1_3:+64542257	None:intergenic
AAGCCTATTGATATAGTAGA+TGG	0.426272	1_3:-64542997	Msa0133280:CDS
TCCTTATGTAGAACAATAGT+GGG	0.435174	1_3:+64542898	None:intergenic
GCTTCTAAGAAGTCAAATAC+TGG	0.435698	1_3:-64542814	Msa0133280:CDS
CCAGCAATAATTGCAATCAT+AGG	0.437264	1_3:+64541800	None:intergenic
ATCAATAGGCTTCACTGCAT+TGG	0.447906	1_3:+64543008	None:intergenic
CTTGAAATCAACAAAAGAAC+TGG	0.448659	1_3:-64542844	Msa0133280:CDS
ATTATGAAACATCCGCTCTT+CGG	0.450015	1_3:+64542675	None:intergenic
TGATGCATTCTTGGAATTGA+AGG	0.454318	1_3:-64542332	Msa0133280:CDS
CCCCACTATTGTTCTACATA+AGG	0.466740	1_3:-64542899	Msa0133280:CDS
ACCTTCAGTTTGCTAAAAGC+AGG	0.486566	1_3:+64542450	None:intergenic
AACTGATAATATTGTTGAGA+AGG	0.486615	1_3:-64541890	Msa0133280:three_prime_UTR
AAAATCACAAAGTCATGCAT+TGG	0.496042	1_3:-64542638	Msa0133280:CDS
TATTGTTCTACATAAGGATA+AGG	0.500681	1_3:-64542893	Msa0133280:CDS
ATATTATCAGTTAAAGGTAA+TGG	0.505834	1_3:+64541901	None:intergenic
CACAAAATACTATCAAGAAA+AGG	0.510043	1_3:+64541828	None:intergenic
TGCAGGAAAGATGAAATCTA+GGG	0.516990	1_3:-64543061	Msa0133280:CDS
AATATCTAAAACAAACAGTA+AGG	0.520906	1_3:+64541761	None:intergenic
TAGATTTGGTGTTTGATTGA+CGG	0.521873	1_3:+64542939	None:intergenic
GATGGAAAGAAGGTCTTCAA+TGG	0.530258	1_3:-64542979	Msa0133280:CDS
ATTTGGTGTTTGATTGACGG+AGG	0.538176	1_3:+64542942	None:intergenic
TAAGGAAGTTGTTTCTGCCA+AGG	0.545987	1_3:-64542875	Msa0133280:CDS
TGAAATTGCAATCGAAAGCT+GGG	0.549449	1_3:+64542206	None:intergenic
AGAAGTCAAATACTGGCAGC+AGG	0.552216	1_3:-64542807	Msa0133280:CDS
TTTGGTGTTTGATTGACGGA+GGG	0.556271	1_3:+64542943	None:intergenic
CTTTAAAAGAACTAGTGTCC+AGG	0.560073	1_3:+64542725	None:intergenic
TTTCAAGACTGCTATACTTG+GGG	0.563438	1_3:-64542602	Msa0133280:CDS
AAGGAAGTTGTTTCTGCCAA+GGG	0.563895	1_3:-64542874	Msa0133280:CDS
TGATATAGTAGATGGAAAGA+AGG	0.564340	1_3:-64542989	Msa0133280:CDS
GGAAAGATGAAATCTAGGGC+TGG	0.572546	1_3:-64543057	Msa0133280:CDS
GGGGAACCATGCTTAGCAGA+TGG	0.575621	1_3:+64542277	None:intergenic
CCGTCTCCATCTGCTAAGCA+TGG	0.588140	1_3:-64542283	Msa0133280:CDS
AAGACTGCTATACTTGGGGA+TGG	0.598286	1_3:-64542598	Msa0133280:intron
CTGATGCAATCGCCGAAGAG+CGG	0.620812	1_3:-64542687	Msa0133280:CDS
AATAATTGCCATCACAAGAG+TGG	0.626500	1_3:+64542102	None:intergenic
AATAATTGCAATCATAGGAG+TGG	0.634076	1_3:+64541805	None:intergenic
CCATGCTTAGCAGATGGAGA+CGG	0.635945	1_3:+64542283	None:intergenic
AATAGATGCCAACACAAGAG+TGG	0.651321	1_3:+64541956	None:intergenic
AAGATGAAATCTAGGGCTGG+AGG	0.656807	1_3:-64543054	Msa0133280:CDS
ATATTGTGCCACTCTTGTGA+TGG	0.665318	1_3:-64542110	Msa0133280:three_prime_UTR
TGGACTCTTGCAGCATACTG+GGG	0.672423	1_3:+64542258	None:intergenic
GCTGGAGGAAGAAAACCACT+TGG	0.686170	1_3:-64543039	Msa0133280:CDS
TTAAAGGTAATGGCTAAACA+AGG	0.692527	1_3:+64541911	None:intergenic
CCTTATGTAGAACAATAGTG+GGG	0.709845	1_3:+64542899	None:intergenic
GCATTGGATAGATCACCAAG+TGG	0.747744	1_3:+64543024	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAATCATATATTGTAAAA+GGG	+	chr1_3:64542131-64542150	None:intergenic	10.0%
!!	AAAAATCATATATTGTAAAA+GGG	+	chr1_3:64542131-64542150	None:intergenic	10.0%
!!	CAAAAATCATATATTGTAAA+AGG	+	chr1_3:64542132-64542151	None:intergenic	15.0%
!!	AATAGTTAAAAAATCAAAAC+AGG	+	chr1_3:64543496-64543515	None:intergenic	15.0%
!!!	GTTTTGATTTTTTAACTATT+TGG	-	chr1_3:64543496-64543515	Msa0133280:intron	15.0%
!!!	TTTTAGATATTATCTTAGAT+TGG	-	chr1_3:64544120-64544139	Msa0133280:five_prime_UTR	15.0%
!!	CAAAAATCATATATTGTAAA+AGG	+	chr1_3:64542132-64542151	None:intergenic	15.0%
!!	AATAGTTAAAAAATCAAAAC+AGG	+	chr1_3:64543496-64543515	None:intergenic	15.0%
!!!	GTTTTGATTTTTTAACTATT+TGG	-	chr1_3:64543496-64543515	Msa0133280:intron	15.0%
!!!	TTTTAGATATTATCTTAGAT+TGG	-	chr1_3:64544120-64544139	Msa0133280:five_prime_UTR	15.0%
!!	ATTCTTTCTTTAATGTTGAT+TGG	-	chr1_3:64541834-64541853	Msa0133280:three_prime_UTR	20.0%
!!!	AAAGAAATGTATTGATTGTT+GGG	-	chr1_3:64541953-64541972	Msa0133280:three_prime_UTR	20.0%
!!!	TATGATTTTTGTGTTTACAT+TGG	-	chr1_3:64542016-64542035	Msa0133280:three_prime_UTR	20.0%
!!!	AAATCGTAAGTTTTCTTTTT+GGG	-	chr1_3:64542184-64542203	Msa0133280:CDS	20.0%
!!!	CAATTATTACTGTATTCTTT+TGG	-	chr1_3:64543782-64543801	Msa0133280:intron	20.0%
!!	GATAATATTGCTTAGAATAT+TGG	-	chr1_3:64543868-64543887	Msa0133280:intron	20.0%
!!!	ATCTATTGCTGTATTTTTTT+GGG	-	chr1_3:64543929-64543948	Msa0133280:intron	20.0%
!!	ATATTATCAGTTAAAGGTAA+TGG	+	chr1_3:64543971-64543990	None:intergenic	20.0%
!!	TCAACAATATTATCAGTTAA+AGG	+	chr1_3:64543977-64543996	None:intergenic	20.0%
!!!	AATATCTAAAACAAACAGTA+AGG	+	chr1_3:64544111-64544130	None:intergenic	20.0%
!!	ATTCTTTCTTTAATGTTGAT+TGG	-	chr1_3:64541834-64541853	Msa0133280:three_prime_UTR	20.0%
!!!	AAAGAAATGTATTGATTGTT+GGG	-	chr1_3:64541953-64541972	Msa0133280:three_prime_UTR	20.0%
!!!	TATGATTTTTGTGTTTACAT+TGG	-	chr1_3:64542016-64542035	Msa0133280:three_prime_UTR	20.0%
!!!	AAATCGTAAGTTTTCTTTTT+GGG	-	chr1_3:64542184-64542203	Msa0133280:CDS	20.0%
!!!	CAATTATTACTGTATTCTTT+TGG	-	chr1_3:64543782-64543801	Msa0133280:intron	20.0%
!!	GATAATATTGCTTAGAATAT+TGG	-	chr1_3:64543868-64543887	Msa0133280:intron	20.0%
!!!	ATCTATTGCTGTATTTTTTT+GGG	-	chr1_3:64543929-64543948	Msa0133280:intron	20.0%
!!	ATATTATCAGTTAAAGGTAA+TGG	+	chr1_3:64543971-64543990	None:intergenic	20.0%
!!	TCAACAATATTATCAGTTAA+AGG	+	chr1_3:64543977-64543996	None:intergenic	20.0%
!!!	AATATCTAAAACAAACAGTA+AGG	+	chr1_3:64544111-64544130	None:intergenic	20.0%
!!	TTTCTTTAATGTTGATTGGT+AGG	-	chr1_3:64541838-64541857	Msa0133280:three_prime_UTR	25.0%
!!!	CAAAGAAATGTATTGATTGT+TGG	-	chr1_3:64541952-64541971	Msa0133280:three_prime_UTR	25.0%
!!!	TTTACATTGGATTTTGATGA+AGG	-	chr1_3:64542029-64542048	Msa0133280:three_prime_UTR	25.0%
!!	GTTTACATTCAAGTTAGATA+AGG	-	chr1_3:64542153-64542172	Msa0133280:exon	25.0%
!!	GAAATCGTAAGTTTTCTTTT+TGG	-	chr1_3:64542183-64542202	Msa0133280:CDS	25.0%
!!	GGTTTCAAACTTTAGATTTT+GGG	-	chr1_3:64542205-64542224	Msa0133280:CDS	25.0%
!!!	TATGTGAAAGTTTTCTAAGA+TGG	-	chr1_3:64542545-64542564	Msa0133280:intron	25.0%
!!	TAACATGATCAATGACATTT+TGG	+	chr1_3:64542663-64542682	None:intergenic	25.0%
!	AATGTCATTGATCATGTTAA+TGG	-	chr1_3:64542664-64542683	Msa0133280:CDS	25.0%
!!	TCATGTTAATGGTATTTTTC+TGG	-	chr1_3:64542675-64542694	Msa0133280:CDS	25.0%
!	AATTGTTTATGTGTGTAGAA+TGG	-	chr1_3:64542727-64542746	Msa0133280:CDS	25.0%
!	TTTCCATCTACTATATCAAT+AGG	+	chr1_3:64542878-64542897	None:intergenic	25.0%
!	TATTGTTCTACATAAGGATA+AGG	-	chr1_3:64542976-64542995	Msa0133280:CDS	25.0%
!	TGTACCTCATATGAAAAATA+AGG	-	chr1_3:64543117-64543136	Msa0133280:intron	25.0%
!!	AGATTCTGTTATGGTAATTT+TGG	-	chr1_3:64543316-64543335	Msa0133280:intron	25.0%
!	ATACATAACTTGATCAGATT+TGG	-	chr1_3:64543367-64543386	Msa0133280:intron	25.0%
!	ATTGAAATCCTTGAAAATTC+TGG	+	chr1_3:64543634-64543653	None:intergenic	25.0%
!!!	AATTTTCAAGGATTTCAATG+TGG	-	chr1_3:64543635-64543654	Msa0133280:intron	25.0%
!!!	ATTTTCAAGGATTTCAATGT+GGG	-	chr1_3:64543636-64543655	Msa0133280:intron	25.0%
!!!	AATCAGTTTTTAGACGATTT+GGG	+	chr1_3:64543703-64543722	None:intergenic	25.0%
!!!	CATCTATTGCTGTATTTTTT+TGG	-	chr1_3:64543928-64543947	Msa0133280:intron	25.0%
!	AACTGATAATATTGTTGAGA+AGG	-	chr1_3:64543979-64543998	Msa0133280:intron	25.0%
!	CACAAAATACTATCAAGAAA+AGG	+	chr1_3:64544044-64544063	None:intergenic	25.0%
!!	TTTCTTTAATGTTGATTGGT+AGG	-	chr1_3:64541838-64541857	Msa0133280:three_prime_UTR	25.0%
!!!	CAAAGAAATGTATTGATTGT+TGG	-	chr1_3:64541952-64541971	Msa0133280:three_prime_UTR	25.0%
!!!	TTTACATTGGATTTTGATGA+AGG	-	chr1_3:64542029-64542048	Msa0133280:three_prime_UTR	25.0%
!!	GTTTACATTCAAGTTAGATA+AGG	-	chr1_3:64542153-64542172	Msa0133280:exon	25.0%
!!	GAAATCGTAAGTTTTCTTTT+TGG	-	chr1_3:64542183-64542202	Msa0133280:CDS	25.0%
!!	GGTTTCAAACTTTAGATTTT+GGG	-	chr1_3:64542205-64542224	Msa0133280:CDS	25.0%
!!!	TATGTGAAAGTTTTCTAAGA+TGG	-	chr1_3:64542545-64542564	Msa0133280:intron	25.0%
!!	TAACATGATCAATGACATTT+TGG	+	chr1_3:64542663-64542682	None:intergenic	25.0%
!	AATGTCATTGATCATGTTAA+TGG	-	chr1_3:64542664-64542683	Msa0133280:CDS	25.0%
!!	TCATGTTAATGGTATTTTTC+TGG	-	chr1_3:64542675-64542694	Msa0133280:CDS	25.0%
!	AATTGTTTATGTGTGTAGAA+TGG	-	chr1_3:64542727-64542746	Msa0133280:CDS	25.0%
!	TTTCCATCTACTATATCAAT+AGG	+	chr1_3:64542878-64542897	None:intergenic	25.0%
!	TATTGTTCTACATAAGGATA+AGG	-	chr1_3:64542976-64542995	Msa0133280:CDS	25.0%
!	TGTACCTCATATGAAAAATA+AGG	-	chr1_3:64543117-64543136	Msa0133280:intron	25.0%
!!	AGATTCTGTTATGGTAATTT+TGG	-	chr1_3:64543316-64543335	Msa0133280:intron	25.0%
!	ATACATAACTTGATCAGATT+TGG	-	chr1_3:64543367-64543386	Msa0133280:intron	25.0%
!	ATTGAAATCCTTGAAAATTC+TGG	+	chr1_3:64543634-64543653	None:intergenic	25.0%
!!!	AATTTTCAAGGATTTCAATG+TGG	-	chr1_3:64543635-64543654	Msa0133280:intron	25.0%
!!!	ATTTTCAAGGATTTCAATGT+GGG	-	chr1_3:64543636-64543655	Msa0133280:intron	25.0%
!!!	AATCAGTTTTTAGACGATTT+GGG	+	chr1_3:64543703-64543722	None:intergenic	25.0%
!!!	CATCTATTGCTGTATTTTTT+TGG	-	chr1_3:64543928-64543947	Msa0133280:intron	25.0%
!	AACTGATAATATTGTTGAGA+AGG	-	chr1_3:64543979-64543998	Msa0133280:intron	25.0%
!	CACAAAATACTATCAAGAAA+AGG	+	chr1_3:64544044-64544063	None:intergenic	25.0%
!	GGGTTTCAAACTTTAGATTT+TGG	-	chr1_3:64542204-64542223	Msa0133280:CDS	30.0%
!!!	AGATTTTGGGTTTCTTTTGA+TGG	-	chr1_3:64542218-64542237	Msa0133280:CDS	30.0%
	TGGTTGGGAAATTTAAATGT+TGG	-	chr1_3:64542238-64542257	Msa0133280:CDS	30.0%
	GGTTGGGAAATTTAAATGTT+GGG	-	chr1_3:64542239-64542258	Msa0133280:CDS	30.0%
	AAATTCTCTGTCTGATTCTT+CGG	+	chr1_3:64542319-64542338	None:intergenic	30.0%
!!	GGACTTTTAATTATGCAGAA+AGG	+	chr1_3:64542345-64542364	None:intergenic	30.0%
	TGAATTCACAACAATGTTCA+TGG	+	chr1_3:64542366-64542385	None:intergenic	30.0%
!	AACCCTTTTACAATATTTGC+AGG	-	chr1_3:64542571-64542590	Msa0133280:intron	30.0%
!	ACCCTTTTACAATATTTGCA+GGG	-	chr1_3:64542572-64542591	Msa0133280:intron	30.0%
	ACCCTGCAAATATTGTAAAA+GGG	+	chr1_3:64542576-64542595	None:intergenic	30.0%
	CTACACACATAAACAATTGA+TGG	+	chr1_3:64542725-64542744	None:intergenic	30.0%
!	TCTATAAAGACAGATTTTGC+AGG	-	chr1_3:64542791-64542810	Msa0133280:CDS	30.0%
	AAGCCTATTGATATAGTAGA+TGG	-	chr1_3:64542872-64542891	Msa0133280:CDS	30.0%
	TGATATAGTAGATGGAAAGA+AGG	-	chr1_3:64542880-64542899	Msa0133280:CDS	30.0%
!!	TAGATTTGGTGTTTGATTGA+CGG	+	chr1_3:64542933-64542952	None:intergenic	30.0%
	TCCTTATGTAGAACAATAGT+GGG	+	chr1_3:64542974-64542993	None:intergenic	30.0%
	ATCCTTATGTAGAACAATAG+TGG	+	chr1_3:64542975-64542994	None:intergenic	30.0%
!!!	GATTTCAAGATTTTTTCCCT+TGG	+	chr1_3:64543014-64543033	None:intergenic	30.0%
	CTTGAAATCAACAAAAGAAC+TGG	-	chr1_3:64543025-64543044	Msa0133280:CDS	30.0%
!!!	CTGTCCTTATTTTTCATATG+AGG	+	chr1_3:64543124-64543143	None:intergenic	30.0%
!!	TGGACACTAGTTCTTTTAAA+GGG	-	chr1_3:64543146-64543165	Msa0133280:intron	30.0%
	AAAATCACAAAGTCATGCAT+TGG	-	chr1_3:64543231-64543250	Msa0133280:CDS	30.0%
!!!	TTTTTCAAGACTGCTATACT+TGG	-	chr1_3:64543265-64543284	Msa0133280:five_prime_UTR	30.0%
!!	TTTTCAAGACTGCTATACTT+GGG	-	chr1_3:64543266-64543285	Msa0133280:five_prime_UTR	30.0%
!	ACTGATTCTAGATTCTGTTA+TGG	-	chr1_3:64543307-64543326	Msa0133280:intron	30.0%
!!	GAATCAGTTTTTAGACGATT+TGG	+	chr1_3:64543704-64543723	None:intergenic	30.0%
!!	TCTTTTGGAGATGTCTTTAA+TGG	-	chr1_3:64543797-64543816	Msa0133280:intron	30.0%
!	TTAAAGGTAATGGCTAAACA+AGG	+	chr1_3:64543961-64543980	None:intergenic	30.0%
	AGCACTCATTCACATAATTT+GGG	+	chr1_3:64544018-64544037	None:intergenic	30.0%
	AAGCACTCATTCACATAATT+TGG	+	chr1_3:64544019-64544038	None:intergenic	30.0%
	AATAATTGCAATCATAGGAG+TGG	+	chr1_3:64544067-64544086	None:intergenic	30.0%
!	GGGTTTCAAACTTTAGATTT+TGG	-	chr1_3:64542204-64542223	Msa0133280:CDS	30.0%
!!!	AGATTTTGGGTTTCTTTTGA+TGG	-	chr1_3:64542218-64542237	Msa0133280:CDS	30.0%
	TGGTTGGGAAATTTAAATGT+TGG	-	chr1_3:64542238-64542257	Msa0133280:CDS	30.0%
	GGTTGGGAAATTTAAATGTT+GGG	-	chr1_3:64542239-64542258	Msa0133280:CDS	30.0%
	AAATTCTCTGTCTGATTCTT+CGG	+	chr1_3:64542319-64542338	None:intergenic	30.0%
!!	GGACTTTTAATTATGCAGAA+AGG	+	chr1_3:64542345-64542364	None:intergenic	30.0%
	TGAATTCACAACAATGTTCA+TGG	+	chr1_3:64542366-64542385	None:intergenic	30.0%
!	AACCCTTTTACAATATTTGC+AGG	-	chr1_3:64542571-64542590	Msa0133280:intron	30.0%
!	ACCCTTTTACAATATTTGCA+GGG	-	chr1_3:64542572-64542591	Msa0133280:intron	30.0%
	ACCCTGCAAATATTGTAAAA+GGG	+	chr1_3:64542576-64542595	None:intergenic	30.0%
	CTACACACATAAACAATTGA+TGG	+	chr1_3:64542725-64542744	None:intergenic	30.0%
!	TCTATAAAGACAGATTTTGC+AGG	-	chr1_3:64542791-64542810	Msa0133280:CDS	30.0%
	AAGCCTATTGATATAGTAGA+TGG	-	chr1_3:64542872-64542891	Msa0133280:CDS	30.0%
	TGATATAGTAGATGGAAAGA+AGG	-	chr1_3:64542880-64542899	Msa0133280:CDS	30.0%
!!	TAGATTTGGTGTTTGATTGA+CGG	+	chr1_3:64542933-64542952	None:intergenic	30.0%
	TCCTTATGTAGAACAATAGT+GGG	+	chr1_3:64542974-64542993	None:intergenic	30.0%
	ATCCTTATGTAGAACAATAG+TGG	+	chr1_3:64542975-64542994	None:intergenic	30.0%
!!!	GATTTCAAGATTTTTTCCCT+TGG	+	chr1_3:64543014-64543033	None:intergenic	30.0%
	CTTGAAATCAACAAAAGAAC+TGG	-	chr1_3:64543025-64543044	Msa0133280:CDS	30.0%
!!!	CTGTCCTTATTTTTCATATG+AGG	+	chr1_3:64543124-64543143	None:intergenic	30.0%
!!	TGGACACTAGTTCTTTTAAA+GGG	-	chr1_3:64543146-64543165	Msa0133280:intron	30.0%
	AAAATCACAAAGTCATGCAT+TGG	-	chr1_3:64543231-64543250	Msa0133280:CDS	30.0%
!!!	TTTTTCAAGACTGCTATACT+TGG	-	chr1_3:64543265-64543284	Msa0133280:five_prime_UTR	30.0%
!!	TTTTCAAGACTGCTATACTT+GGG	-	chr1_3:64543266-64543285	Msa0133280:five_prime_UTR	30.0%
!	ACTGATTCTAGATTCTGTTA+TGG	-	chr1_3:64543307-64543326	Msa0133280:intron	30.0%
!!	GAATCAGTTTTTAGACGATT+TGG	+	chr1_3:64543704-64543723	None:intergenic	30.0%
!!	TCTTTTGGAGATGTCTTTAA+TGG	-	chr1_3:64543797-64543816	Msa0133280:intron	30.0%
!	TTAAAGGTAATGGCTAAACA+AGG	+	chr1_3:64543961-64543980	None:intergenic	30.0%
	AGCACTCATTCACATAATTT+GGG	+	chr1_3:64544018-64544037	None:intergenic	30.0%
	AAGCACTCATTCACATAATT+TGG	+	chr1_3:64544019-64544038	None:intergenic	30.0%
	AATAATTGCAATCATAGGAG+TGG	+	chr1_3:64544067-64544086	None:intergenic	30.0%
!	GAAACCCTAGGTTTTCATTT+GGG	-	chr1_3:64542058-64542077	Msa0133280:three_prime_UTR	35.0%
!	AAACCCTAGGTTTTCATTTG+GGG	-	chr1_3:64542059-64542078	Msa0133280:three_prime_UTR	35.0%
!!	TTTGGGTTTCTTTTGATGGT+TGG	-	chr1_3:64542222-64542241	Msa0133280:CDS	35.0%
!!	TTGGGTTTCTTTTGATGGTT+GGG	-	chr1_3:64542223-64542242	Msa0133280:CDS	35.0%
	CACCCTGCAAATATTGTAAA+AGG	+	chr1_3:64542577-64542596	None:intergenic	35.0%
!	TAGCTACTTTAACCGCAAAA+TGG	-	chr1_3:64542609-64542628	Msa0133280:CDS	35.0%
	TTTATGTGTGTAGAATGGCT+AGG	-	chr1_3:64542732-64542751	Msa0133280:CDS	35.0%
	TTGCAGGAAAGATGAAATCT+AGG	-	chr1_3:64542807-64542826	Msa0133280:CDS	35.0%
	TGCAGGAAAGATGAAATCTA+GGG	-	chr1_3:64542808-64542827	Msa0133280:CDS	35.0%
	TTCTTGGACTTGAGTAGATT+TGG	+	chr1_3:64542947-64542966	None:intergenic	35.0%
	CCTTATGTAGAACAATAGTG+GGG	+	chr1_3:64542973-64542992	None:intergenic	35.0%
!	GCTTCTAAGAAGTCAAATAC+TGG	-	chr1_3:64543055-64543074	Msa0133280:CDS	35.0%
	TATGAAAAATAAGGACAGCC+TGG	-	chr1_3:64543126-64543145	Msa0133280:intron	35.0%
!	CTTTAAAAGAACTAGTGTCC+AGG	+	chr1_3:64543147-64543166	None:intergenic	35.0%
!!	CTGGACACTAGTTCTTTTAA+AGG	-	chr1_3:64543145-64543164	Msa0133280:intron	35.0%
	ATTATGAAACATCCGCTCTT+CGG	+	chr1_3:64543197-64543216	None:intergenic	35.0%
	TTTCAAGACTGCTATACTTG+GGG	-	chr1_3:64543267-64543286	Msa0133280:five_prime_UTR	35.0%
	TGATGCATTCTTGGAATTGA+AGG	-	chr1_3:64543537-64543556	Msa0133280:intron	35.0%
	TGAAATTGCAATCGAAAGCT+GGG	+	chr1_3:64543666-64543685	None:intergenic	35.0%
	TGTGTACTTCATATTGTTGC+AGG	+	chr1_3:64543730-64543749	None:intergenic	35.0%
	AATAATTGCCATCACAAGAG+TGG	+	chr1_3:64543770-64543789	None:intergenic	35.0%
	CCAGCAATAATTGCAATCAT+AGG	+	chr1_3:64544072-64544091	None:intergenic	35.0%
	CCTATGATTGCAATTATTGC+TGG	-	chr1_3:64544069-64544088	Msa0133280:intron	35.0%
!	GAAACCCTAGGTTTTCATTT+GGG	-	chr1_3:64542058-64542077	Msa0133280:three_prime_UTR	35.0%
!	AAACCCTAGGTTTTCATTTG+GGG	-	chr1_3:64542059-64542078	Msa0133280:three_prime_UTR	35.0%
!!	TTTGGGTTTCTTTTGATGGT+TGG	-	chr1_3:64542222-64542241	Msa0133280:CDS	35.0%
!!	TTGGGTTTCTTTTGATGGTT+GGG	-	chr1_3:64542223-64542242	Msa0133280:CDS	35.0%
	CACCCTGCAAATATTGTAAA+AGG	+	chr1_3:64542577-64542596	None:intergenic	35.0%
!	TAGCTACTTTAACCGCAAAA+TGG	-	chr1_3:64542609-64542628	Msa0133280:CDS	35.0%
	TTTATGTGTGTAGAATGGCT+AGG	-	chr1_3:64542732-64542751	Msa0133280:CDS	35.0%
	TTGCAGGAAAGATGAAATCT+AGG	-	chr1_3:64542807-64542826	Msa0133280:CDS	35.0%
	TGCAGGAAAGATGAAATCTA+GGG	-	chr1_3:64542808-64542827	Msa0133280:CDS	35.0%
	TTCTTGGACTTGAGTAGATT+TGG	+	chr1_3:64542947-64542966	None:intergenic	35.0%
	CCTTATGTAGAACAATAGTG+GGG	+	chr1_3:64542973-64542992	None:intergenic	35.0%
!	GCTTCTAAGAAGTCAAATAC+TGG	-	chr1_3:64543055-64543074	Msa0133280:CDS	35.0%
	TATGAAAAATAAGGACAGCC+TGG	-	chr1_3:64543126-64543145	Msa0133280:intron	35.0%
!	CTTTAAAAGAACTAGTGTCC+AGG	+	chr1_3:64543147-64543166	None:intergenic	35.0%
!!	CTGGACACTAGTTCTTTTAA+AGG	-	chr1_3:64543145-64543164	Msa0133280:intron	35.0%
	ATTATGAAACATCCGCTCTT+CGG	+	chr1_3:64543197-64543216	None:intergenic	35.0%
	TTTCAAGACTGCTATACTTG+GGG	-	chr1_3:64543267-64543286	Msa0133280:five_prime_UTR	35.0%
	TGATGCATTCTTGGAATTGA+AGG	-	chr1_3:64543537-64543556	Msa0133280:intron	35.0%
	TGAAATTGCAATCGAAAGCT+GGG	+	chr1_3:64543666-64543685	None:intergenic	35.0%
	TGTGTACTTCATATTGTTGC+AGG	+	chr1_3:64543730-64543749	None:intergenic	35.0%
	AATAATTGCCATCACAAGAG+TGG	+	chr1_3:64543770-64543789	None:intergenic	35.0%
	CCAGCAATAATTGCAATCAT+AGG	+	chr1_3:64544072-64544091	None:intergenic	35.0%
	CCTATGATTGCAATTATTGC+TGG	-	chr1_3:64544069-64544088	Msa0133280:intron	35.0%
!	TTCCACTGTTTGTTAATCGC+TGG	-	chr1_3:64541890-64541909	Msa0133280:three_prime_UTR	40.0%
	TTCCAGCGATTAACAAACAG+TGG	+	chr1_3:64541895-64541914	None:intergenic	40.0%
	TGAAGGAAAATCGAAACCCT+AGG	-	chr1_3:64542046-64542065	Msa0133280:three_prime_UTR	40.0%
!	CGAAACCCTAGGTTTTCATT+TGG	-	chr1_3:64542057-64542076	Msa0133280:three_prime_UTR	40.0%
	AAGCCCCAAATGAAAACCTA+GGG	+	chr1_3:64542065-64542084	None:intergenic	40.0%
!	TTCATTTGGGGCTTCACAAT+TGG	-	chr1_3:64542071-64542090	Msa0133280:three_prime_UTR	40.0%
!	TCATTTGGGGCTTCACAATT+GGG	-	chr1_3:64542072-64542091	Msa0133280:three_prime_UTR	40.0%
!	GGCTTCACAATTGGGATTTT+GGG	-	chr1_3:64542080-64542099	Msa0133280:three_prime_UTR	40.0%
!	GCTTCACAATTGGGATTTTG+GGG	-	chr1_3:64542081-64542100	Msa0133280:three_prime_UTR	40.0%
!!!	GGATTTTGGGGTTTCTTTTG+TGG	-	chr1_3:64542093-64542112	Msa0133280:three_prime_UTR	40.0%
	ATCAATAGGCTTCACTGCAT+TGG	+	chr1_3:64542864-64542883	None:intergenic	40.0%
	GATGGAAAGAAGGTCTTCAA+TGG	-	chr1_3:64542890-64542909	Msa0133280:CDS	40.0%
!!	TTTGGTGTTTGATTGACGGA+GGG	+	chr1_3:64542929-64542948	None:intergenic	40.0%
!!	ATTTGGTGTTTGATTGACGG+AGG	+	chr1_3:64542930-64542949	None:intergenic	40.0%
!	AACAATAGTGGGGTTGTTCT+TGG	+	chr1_3:64542963-64542982	None:intergenic	40.0%
	CCCCACTATTGTTCTACATA+AGG	-	chr1_3:64542970-64542989	Msa0133280:CDS	40.0%
!!	TAAGGAAGTTGTTTCTGCCA+AGG	-	chr1_3:64542994-64543013	Msa0133280:CDS	40.0%
!!	AAGGAAGTTGTTTCTGCCAA+GGG	-	chr1_3:64542995-64543014	Msa0133280:CDS	40.0%
!!	ACCTTCAGTTTGCTAAAAGC+AGG	+	chr1_3:64543422-64543441	None:intergenic	40.0%
	CATCATCAGACTGAAAAGCA+GGG	+	chr1_3:64543522-64543541	None:intergenic	40.0%
!	TCAGTCTGATGATGCATTCT+TGG	-	chr1_3:64543528-64543547	Msa0133280:intron	40.0%
!	GCAAGAGTCCAGAATTTTCA+AGG	-	chr1_3:64543623-64543642	Msa0133280:intron	40.0%
	GTGAAATTGCAATCGAAAGC+TGG	+	chr1_3:64543667-64543686	None:intergenic	40.0%
!	ATATTGTGCCACTCTTGTGA+TGG	-	chr1_3:64543759-64543778	Msa0133280:intron	40.0%
!!	ATTTTGTGCCACTCTTGTGT+TGG	-	chr1_3:64543905-64543924	Msa0133280:intron	40.0%
	AATAGATGCCAACACAAGAG+TGG	+	chr1_3:64543916-64543935	None:intergenic	40.0%
!	TTCCACTGTTTGTTAATCGC+TGG	-	chr1_3:64541890-64541909	Msa0133280:three_prime_UTR	40.0%
	TTCCAGCGATTAACAAACAG+TGG	+	chr1_3:64541895-64541914	None:intergenic	40.0%
	TGAAGGAAAATCGAAACCCT+AGG	-	chr1_3:64542046-64542065	Msa0133280:three_prime_UTR	40.0%
!	CGAAACCCTAGGTTTTCATT+TGG	-	chr1_3:64542057-64542076	Msa0133280:three_prime_UTR	40.0%
	AAGCCCCAAATGAAAACCTA+GGG	+	chr1_3:64542065-64542084	None:intergenic	40.0%
!	TTCATTTGGGGCTTCACAAT+TGG	-	chr1_3:64542071-64542090	Msa0133280:three_prime_UTR	40.0%
!	TCATTTGGGGCTTCACAATT+GGG	-	chr1_3:64542072-64542091	Msa0133280:three_prime_UTR	40.0%
!	GGCTTCACAATTGGGATTTT+GGG	-	chr1_3:64542080-64542099	Msa0133280:three_prime_UTR	40.0%
!	GCTTCACAATTGGGATTTTG+GGG	-	chr1_3:64542081-64542100	Msa0133280:three_prime_UTR	40.0%
!!!	GGATTTTGGGGTTTCTTTTG+TGG	-	chr1_3:64542093-64542112	Msa0133280:three_prime_UTR	40.0%
	ATCAATAGGCTTCACTGCAT+TGG	+	chr1_3:64542864-64542883	None:intergenic	40.0%
	GATGGAAAGAAGGTCTTCAA+TGG	-	chr1_3:64542890-64542909	Msa0133280:CDS	40.0%
!!	TTTGGTGTTTGATTGACGGA+GGG	+	chr1_3:64542929-64542948	None:intergenic	40.0%
!!	ATTTGGTGTTTGATTGACGG+AGG	+	chr1_3:64542930-64542949	None:intergenic	40.0%
!	AACAATAGTGGGGTTGTTCT+TGG	+	chr1_3:64542963-64542982	None:intergenic	40.0%
	CCCCACTATTGTTCTACATA+AGG	-	chr1_3:64542970-64542989	Msa0133280:CDS	40.0%
!!	TAAGGAAGTTGTTTCTGCCA+AGG	-	chr1_3:64542994-64543013	Msa0133280:CDS	40.0%
!!	AAGGAAGTTGTTTCTGCCAA+GGG	-	chr1_3:64542995-64543014	Msa0133280:CDS	40.0%
!!	ACCTTCAGTTTGCTAAAAGC+AGG	+	chr1_3:64543422-64543441	None:intergenic	40.0%
	CATCATCAGACTGAAAAGCA+GGG	+	chr1_3:64543522-64543541	None:intergenic	40.0%
!	TCAGTCTGATGATGCATTCT+TGG	-	chr1_3:64543528-64543547	Msa0133280:intron	40.0%
!	GCAAGAGTCCAGAATTTTCA+AGG	-	chr1_3:64543623-64543642	Msa0133280:intron	40.0%
	GTGAAATTGCAATCGAAAGC+TGG	+	chr1_3:64543667-64543686	None:intergenic	40.0%
!	ATATTGTGCCACTCTTGTGA+TGG	-	chr1_3:64543759-64543778	Msa0133280:intron	40.0%
!!	ATTTTGTGCCACTCTTGTGT+TGG	-	chr1_3:64543905-64543924	Msa0133280:intron	40.0%
	AATAGATGCCAACACAAGAG+TGG	+	chr1_3:64543916-64543935	None:intergenic	40.0%
!	GGGTTTGTCTGCGCTTTATT+AGG	-	chr1_3:64541973-64541992	Msa0133280:three_prime_UTR	45.0%
	GAAGCCCCAAATGAAAACCT+AGG	+	chr1_3:64542066-64542085	None:intergenic	45.0%
!	GGGCTTCACAATTGGGATTT+TGG	-	chr1_3:64542079-64542098	Msa0133280:three_prime_UTR	45.0%
!	GCTAGGCTGATTCTTAATGC+TGG	-	chr1_3:64542749-64542768	Msa0133280:CDS	45.0%
!	GGAAAGATGAAATCTAGGGC+TGG	-	chr1_3:64542812-64542831	Msa0133280:CDS	45.0%
!	AAGATGAAATCTAGGGCTGG+AGG	-	chr1_3:64542815-64542834	Msa0133280:CDS	45.0%
	GCATTGGATAGATCACCAAG+TGG	+	chr1_3:64542848-64542867	None:intergenic	45.0%
	AGAAGTCAAATACTGGCAGC+AGG	-	chr1_3:64543062-64543081	Msa0133280:CDS	45.0%
	AAGACTGCTATACTTGGGGA+TGG	-	chr1_3:64543271-64543290	Msa0133280:five_prime_UTR	45.0%
!!	GCCTGCTTTTAGCAAACTGA+AGG	-	chr1_3:64543418-64543437	Msa0133280:intron	45.0%
	GCATCATCAGACTGAAAAGC+AGG	+	chr1_3:64543523-64543542	None:intergenic	45.0%
!	GGGTTTGTCTGCGCTTTATT+AGG	-	chr1_3:64541973-64541992	Msa0133280:three_prime_UTR	45.0%
	GAAGCCCCAAATGAAAACCT+AGG	+	chr1_3:64542066-64542085	None:intergenic	45.0%
!	GGGCTTCACAATTGGGATTT+TGG	-	chr1_3:64542079-64542098	Msa0133280:three_prime_UTR	45.0%
!	GCTAGGCTGATTCTTAATGC+TGG	-	chr1_3:64542749-64542768	Msa0133280:CDS	45.0%
!	GGAAAGATGAAATCTAGGGC+TGG	-	chr1_3:64542812-64542831	Msa0133280:CDS	45.0%
!	AAGATGAAATCTAGGGCTGG+AGG	-	chr1_3:64542815-64542834	Msa0133280:CDS	45.0%
	GCATTGGATAGATCACCAAG+TGG	+	chr1_3:64542848-64542867	None:intergenic	45.0%
	AGAAGTCAAATACTGGCAGC+AGG	-	chr1_3:64543062-64543081	Msa0133280:CDS	45.0%
	AAGACTGCTATACTTGGGGA+TGG	-	chr1_3:64543271-64543290	Msa0133280:five_prime_UTR	45.0%
!!	GCCTGCTTTTAGCAAACTGA+AGG	-	chr1_3:64543418-64543437	Msa0133280:intron	45.0%
	GCATCATCAGACTGAAAAGC+AGG	+	chr1_3:64543523-64543542	None:intergenic	45.0%
!!!	TTCTTTATTTTATTTTATTT+TGG	-	chr1_3:64542263-64542282	Msa0133280:CDS	5.0%
!!!	TTCTTTATTTTATTTTATTT+TGG	-	chr1_3:64542263-64542282	Msa0133280:CDS	5.0%
!!	CAGTTCGTGCAGCCATTTTG+CGG	+	chr1_3:64542624-64542643	None:intergenic	50.0%
	CTTAATGCTGGTGCAGATGC+AGG	-	chr1_3:64542761-64542780	Msa0133280:CDS	50.0%
	GCTGGAGGAAGAAAACCACT+TGG	-	chr1_3:64542830-64542849	Msa0133280:CDS	50.0%
	GGCATATCAGGCAACTCTTC+AGG	+	chr1_3:64543568-64543587	None:intergenic	50.0%
	CCATGCTTAGCAGATGGAGA+CGG	+	chr1_3:64543589-64543608	None:intergenic	50.0%
	TGGACTCTTGCAGCATACTG+GGG	+	chr1_3:64543614-64543633	None:intergenic	50.0%
	CTGGACTCTTGCAGCATACT+GGG	+	chr1_3:64543615-64543634	None:intergenic	50.0%
	TCTGGACTCTTGCAGCATAC+TGG	+	chr1_3:64543616-64543635	None:intergenic	50.0%
!!	CAGTTCGTGCAGCCATTTTG+CGG	+	chr1_3:64542624-64542643	None:intergenic	50.0%
	CTTAATGCTGGTGCAGATGC+AGG	-	chr1_3:64542761-64542780	Msa0133280:CDS	50.0%
	GCTGGAGGAAGAAAACCACT+TGG	-	chr1_3:64542830-64542849	Msa0133280:CDS	50.0%
	GGCATATCAGGCAACTCTTC+AGG	+	chr1_3:64543568-64543587	None:intergenic	50.0%
	CCATGCTTAGCAGATGGAGA+CGG	+	chr1_3:64543589-64543608	None:intergenic	50.0%
	TGGACTCTTGCAGCATACTG+GGG	+	chr1_3:64543614-64543633	None:intergenic	50.0%
	CTGGACTCTTGCAGCATACT+GGG	+	chr1_3:64543615-64543634	None:intergenic	50.0%
	TCTGGACTCTTGCAGCATAC+TGG	+	chr1_3:64543616-64543635	None:intergenic	50.0%
	CTGATGCAATCGCCGAAGAG+CGG	-	chr1_3:64543182-64543201	Msa0133280:intron	55.0%
	GCAGATGGAGACGGCATATC+AGG	+	chr1_3:64543580-64543599	None:intergenic	55.0%
	CCGTCTCCATCTGCTAAGCA+TGG	-	chr1_3:64543586-64543605	Msa0133280:intron	55.0%
	GGGGAACCATGCTTAGCAGA+TGG	+	chr1_3:64543595-64543614	None:intergenic	55.0%
	CTGATGCAATCGCCGAAGAG+CGG	-	chr1_3:64543182-64543201	Msa0133280:intron	55.0%
	GCAGATGGAGACGGCATATC+AGG	+	chr1_3:64543580-64543599	None:intergenic	55.0%
	CCGTCTCCATCTGCTAAGCA+TGG	-	chr1_3:64543586-64543605	Msa0133280:intron	55.0%
	GGGGAACCATGCTTAGCAGA+TGG	+	chr1_3:64543595-64543614	None:intergenic	55.0%
	CGCTCTTCGGCGATTGCATC+AGG	+	chr1_3:64543184-64543203	None:intergenic	60.0%
	CGCTCTTCGGCGATTGCATC+AGG	+	chr1_3:64543184-64543203	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	64541731	64544160	64541731	ID=Msa0133280;Name=Msa0133280
chr1_3	mRNA	64541731	64544160	64541731	ID=Msa0133280-mRNA-1;Parent=Msa0133280;Name=Msa0133280-mRNA-1;_AED=0.03;_eAED=0.03;_QI=99\|1\|1\|1\|0.75\|0.6\|5\|440\|272
chr1_3	exon	64541731	64542359	64541731	ID=Msa0133280-mRNA-1:exon:21746;Parent=Msa0133280-mRNA-1
chr1_3	exon	64542452	64542507	64542452	ID=Msa0133280-mRNA-1:exon:21745;Parent=Msa0133280-mRNA-1
chr1_3	exon	64542599	64543114	64542599	ID=Msa0133280-mRNA-1:exon:21744;Parent=Msa0133280-mRNA-1
chr1_3	exon	64543206	64543298	64543206	ID=Msa0133280-mRNA-1:exon:21743;Parent=Msa0133280-mRNA-1
chr1_3	exon	64544097	64544160	64544097	ID=Msa0133280-mRNA-1:exon:21742;Parent=Msa0133280-mRNA-1
chr1_3	five_prime_UTR	64544097	64544160	64544097	ID=Msa0133280-mRNA-1:five_prime_utr;Parent=Msa0133280-mRNA-1
chr1_3	five_prime_UTR	64543264	64543298	64543264	ID=Msa0133280-mRNA-1:five_prime_utr;Parent=Msa0133280-mRNA-1
chr1_3	CDS	64543206	64543263	64543206	ID=Msa0133280-mRNA-1:cds;Parent=Msa0133280-mRNA-1
chr1_3	CDS	64542599	64543114	64542599	ID=Msa0133280-mRNA-1:cds;Parent=Msa0133280-mRNA-1
chr1_3	CDS	64542452	64542507	64542452	ID=Msa0133280-mRNA-1:cds;Parent=Msa0133280-mRNA-1
chr1_3	CDS	64542171	64542359	64542171	ID=Msa0133280-mRNA-1:cds;Parent=Msa0133280-mRNA-1
chr1_3	three_prime_UTR	64541731	64542170	64541731	ID=Msa0133280-mRNA-1:three_prime_utr;Parent=Msa0133280-mRNA-1

Gene Sequence

>Msa0133280

ATGGCTGCACGAACTGTTCGTTTGTTTCAAGACCAAAATGTCATTGATCATGTTAATGATGCAGGAACTATGTCTATAAAGACAGATTTTGCAGGAAAGATGAAATCTAGGGCTGGAGGAAGAAAACCACTTGGTGATCTATCCAATGCAGTGAAGCCTATTGATATAGTAGATGGAAAGAAGGTCTTCAATGGTAGCACAATTAAACCCTCCGTCAATCAAACACCAAATCTACTCAAGTCCAAGAACAACCCCACTATTGTTCTACATAAGGATAAGGAAGTTGTTTCTGCCAAGGGAAAAAATCTTGAAATCAACAAAAGAACTGGCAGCAAAGCTTCTAAGAAGTCAAATACTGGCAGCAGGAGAGCACTTACTGACATCTCAAACTCGCTGCATGTACCTCATATGAAAAATAAGGACAGCCTGGACACTAGTTCTTTTAAAGGGAAATATCTTCATCCTGATGCAATCGCCGAAGAGCGGATGTTTCATAATCATGAAGAATGTATAAAATCACAAAGTCATGCATTGGATATGCATCATTTTTTCAAGACTGCTATACTTGGGGATGATTTGGATGATGACATGAAAATTTCATTTGAACAGCCTGCTTTTAGCAAACTGAAGTCTGATGATGCATTCTTGGAATTGAAGGAGATGCCTGAAGAGTTGCCTGATATGCCGTCTCCATCTGCTAAGCATGGTTCCCCAGTATGCTGCAAGAGTCCAGAATTTTCAAGGATTTCAATGTGGGATGACCCAGCTTTCGATTGCAATTTCACTTTGATAGAATCACCCAAATCGTCTAAAAACTGA

Protein sequence

>Msa0133280

MAARTVRLFQDQNVIDHVNDAGTMSIKTDFAGKMKSRAGGRKPLGDLSNAVKPIDIVDGKKVFNGSTIKPSVNQTPNLLKSKNNPTIVLHKDKEVVSAKGKNLEINKRTGSKASKKSNTGSRRALTDISNSLHVPHMKNKDSLDTSSFKGKYLHPDAIAEERMFHNHEECIKSQSHALDMHHFFKTAILGDDLDDDMKISFEQPAFSKLKSDDAFLELKEMPEELPDMPSPSAKHGSPVCCKSPEFSRISMWDDPAFDCNFTLIESPKSSKN*