AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0242280


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0242280 MtrunA17_Chr2g0294391 93.333 105 7 0 3 107 84 188 4.42e-66 205
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 22 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTAGTACTCTTGGTGTTT+CGG 0.308560 2_2:+18373081 None:intergenic
ACATCTTATGCTGCCTTAGA+AGG 0.333220 2_2:-18373476 Msa0242280:CDS
GTGAAGATCTTTATCATCAT+TGG 0.352123 2_2:+18373434 None:intergenic
TATGCTGCCTTAGAAGGCCT+TGG 0.364084 2_2:-18373470 Msa0242280:CDS
TCATCACCCAAGGCCTTCTA+AGG 0.373122 2_2:+18373463 None:intergenic
GTTAAAACCTTTAGTACTCT+TGG 0.395381 2_2:+18373073 None:intergenic
TTTGTGAAGTAGATTTCTCT+TGG 0.408141 2_2:+18373116 None:intergenic
ATGCTGCCTTAGAAGGCCTT+GGG 0.429919 2_2:-18373469 Msa0242280:CDS
TGGACTGAGATTGCATTAGC+AGG 0.444573 2_2:+18373136 None:intergenic
AACGGTACGGAGCGCTGCTA+TGG 0.484247 2_2:-18372941 Msa0242280:CDS
CGAAACACCAAGAGTACTAA+AGG 0.504880 2_2:-18373080 Msa0242280:intron
CAGTCCGATGGACAGTCCTC+GGG 0.520332 2_2:+18373184 None:intergenic
ACAGTCCGATGGACAGTCCT+CGG 0.535368 2_2:+18373183 None:intergenic
CATGCAGGACGGAGTTATAA+CGG 0.542641 2_2:-18372959 Msa0242280:intron
ACGGAGCGCTGCTATGGCTG+TGG 0.552365 2_2:-18372935 Msa0242280:CDS
GTTGTGAGTGCTGTGAATGA+AGG 0.582088 2_2:-18373516 Msa0242280:CDS
GGACTGAGATTGCATTAGCA+GGG 0.593113 2_2:+18373137 None:intergenic
TGATCCCGAGGACTGTCCAT+CGG 0.604988 2_2:-18373188 Msa0242280:CDS
CTTAACAGAATTTGATCCCG+AGG 0.610386 2_2:-18373200 Msa0242280:intron
CAAGGGCGTGAACAGTCCGA+TGG 0.641778 2_2:+18373172 None:intergenic
TTGGCACTGATCATCACCCA+AGG 0.656005 2_2:+18373453 None:intergenic
AGGACGGAGTTATAACGGTA+CGG 0.662658 2_2:-18372954 Msa0242280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAACTTGTGATGTTTAAT+TGG - chr2_2:18373476-18373495 Msa0242280:CDS 20.0%
! AAAAAAAAGAACTGTCTTAC+TGG + chr2_2:18373066-18373085 None:intergenic 25.0%
! AAAGTTTAATCTATGCTTAC+CGG - chr2_2:18373212-18373231 Msa0242280:intron 25.0%
GTGAAGATCTTTATCATCAT+TGG + chr2_2:18373033-18373052 None:intergenic 30.0%
TAACAACATTGAGTGATTAC+CGG + chr2_2:18373234-18373253 None:intergenic 30.0%
TTTGTGAAGTAGATTTCTCT+TGG + chr2_2:18373351-18373370 None:intergenic 30.0%
GTTAAAACCTTTAGTACTCT+TGG + chr2_2:18373394-18373413 None:intergenic 30.0%
TTTAATTGGATTGAACATGC+AGG - chr2_2:18373490-18373509 Msa0242280:CDS 30.0%
!! CTTTAGTACTCTTGGTGTTT+CGG + chr2_2:18373386-18373405 None:intergenic 35.0%
! ATTTTATCATAGGGGCAAAC+TGG + chr2_2:18373565-18373584 None:intergenic 35.0%
ACATCTTATGCTGCCTTAGA+AGG - chr2_2:18372988-18373007 Msa0242280:intron 40.0%
CTTAACAGAATTTGATCCCG+AGG - chr2_2:18373264-18373283 Msa0242280:intron 40.0%
! CAGGGCAAACATTTTCACAA+GGG + chr2_2:18373312-18373331 None:intergenic 40.0%
!! CGAAACACCAAGAGTACTAA+AGG - chr2_2:18373384-18373403 Msa0242280:intron 40.0%
ATTGGATTGAACATGCAGGA+CGG - chr2_2:18373494-18373513 Msa0242280:CDS 40.0%
! GTTGTGAGTGCTGTGAATGA+AGG - chr2_2:18372948-18372967 Msa0242280:CDS 45.0%
GTATGCTGACGTGTGATGTA+TGG + chr2_2:18373157-18373176 None:intergenic 45.0%
! GCAGGGCAAACATTTTCACA+AGG + chr2_2:18373313-18373332 None:intergenic 45.0%
GGACTGAGATTGCATTAGCA+GGG + chr2_2:18373330-18373349 None:intergenic 45.0%
TGGACTGAGATTGCATTAGC+AGG + chr2_2:18373331-18373350 None:intergenic 45.0%
CATGCAGGACGGAGTTATAA+CGG - chr2_2:18373505-18373524 Msa0242280:CDS 45.0%
AGGACGGAGTTATAACGGTA+CGG - chr2_2:18373510-18373529 Msa0242280:CDS 45.0%
TATGCTGCCTTAGAAGGCCT+TGG - chr2_2:18372994-18373013 Msa0242280:intron 50.0%
ATGCTGCCTTAGAAGGCCTT+GGG - chr2_2:18372995-18373014 Msa0242280:intron 50.0%
TCATCACCCAAGGCCTTCTA+AGG + chr2_2:18373004-18373023 None:intergenic 50.0%
!! TTGGCACTGATCATCACCCA+AGG + chr2_2:18373014-18373033 None:intergenic 50.0%
TGATCCCGAGGACTGTCCAT+CGG - chr2_2:18373276-18373295 Msa0242280:intron 55.0%
ACAGTCCGATGGACAGTCCT+CGG + chr2_2:18373284-18373303 None:intergenic 55.0%
CAGTCCGATGGACAGTCCTC+GGG + chr2_2:18373283-18373302 None:intergenic 60.0%
! CAAGGGCGTGAACAGTCCGA+TGG + chr2_2:18373295-18373314 None:intergenic 60.0%
!! AACGGTACGGAGCGCTGCTA+TGG - chr2_2:18373523-18373542 Msa0242280:CDS 60.0%
!! ACGGAGCGCTGCTATGGCTG+TGG - chr2_2:18373529-18373548 Msa0242280:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 18372900 18373586 18372900 ID=Msa0242280;Name=Msa0242280
chr2_2 mRNA 18372900 18373586 18372900 ID=Msa0242280-mRNA-1;Parent=Msa0242280;Name=Msa0242280-mRNA-1;_AED=0.44;_eAED=0.56;_QI=0|0|0|1|1|1|3|0|124
chr2_2 exon 18373421 18373586 18373421 ID=Msa0242280-mRNA-1:exon:7537;Parent=Msa0242280-mRNA-1
chr2_2 exon 18373081 18373214 18373081 ID=Msa0242280-mRNA-1:exon:7536;Parent=Msa0242280-mRNA-1
chr2_2 exon 18372900 18372974 18372900 ID=Msa0242280-mRNA-1:exon:7535;Parent=Msa0242280-mRNA-1
chr2_2 CDS 18373421 18373586 18373421 ID=Msa0242280-mRNA-1:cds;Parent=Msa0242280-mRNA-1
chr2_2 CDS 18373081 18373214 18373081 ID=Msa0242280-mRNA-1:cds;Parent=Msa0242280-mRNA-1
chr2_2 CDS 18372900 18372974 18372900 ID=Msa0242280-mRNA-1:cds;Parent=Msa0242280-mRNA-1
Gene Sequence

>Msa0242280

ATGTGGTTGATTGCAGTTGACTGCATTGACTGTGCTGCTGATGCATCTGTTGTGAGTGCTGTGAATGAAGGAATTGAAGCTGCAAGAGACATCTTATGCTGCCTTAGAAGGCCTTGGGTGATGATCAGTGCCAATGATGATAAAGATCTTCACTTTCGCAAAGCCAAATTTGATCCCGAGGACTGTCCATCGGACTGTTCACGCCCTTGTGAAAATGTTTGCCCTGCTAATGCAATCTCAGTCCAAGAGAAATCTACTTCACAAATTTCATGTAACACCGAAACACCAAGAGTACTAAAGGACGGAGTTATAACGGTACGGAGCGCTGCTATGGCTGTGGTCGCTGTCTTCCAGTTTGCCCCTATGATAAAATAA

Protein sequence

>Msa0242280

MWLIAVDCIDCAADASVVSAVNEGIEAARDILCCLRRPWVMISANDDKDLHFRKAKFDPEDCPSDCSRPCENVCPANAISVQEKSTSQISCNTETPRVLKDGVITVRSAAMAVVAVFQFAPMIK*