AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0411280


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0411280 MtrunA17_Chr3g0102541 92.308 78 6 0 3 80 658 735 2.46e-42 145
Msa0411280 MtrunA17_Chr3g0102531 84.810 79 9 1 3 81 657 732 3.68e-38 134
Msa0411280 MtrunA17_Chr3g0102551 51.282 78 37 1 1 78 661 737 2.98e-20 82.8
Msa0411280 MtrunA17_Chr1g0186381 35.802 81 46 2 1 80 662 737 7.06e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0411280 AT1G55850.1 44.000 75 39 1 1 75 653 724 1.09e-14 67.4

Find 11 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTATCTTTCCTTAGAAAA+AGG 0.222725 3_2:-53486578 None:intergenic
AGCAAGTGTTACATTATTTG+AGG 0.419258 3_2:+53486491 Msa0411280:CDS
ATCAATATACCTATTTATCA+AGG 0.451343 3_2:+53486555 Msa0411280:CDS
TTTAGCTAAGGGCACTATAG+AGG 0.499956 3_2:-53486659 None:intergenic
TCTTGTTGGGATGTTGCTAA+AGG 0.509119 3_2:+53486452 Msa0411280:CDS
TGCAATTACATCAACAACAT+TGG 0.535135 3_2:+53486620 Msa0411280:CDS
CAAGGACATGGATTTAGCTA+AGG 0.541799 3_2:-53486671 None:intergenic
GATGTAATTGCAACAGACCT+TGG 0.567343 3_2:-53486609 None:intergenic
TGTTGGGATGTTGCTAAAGG+AGG 0.567645 3_2:+53486455 Msa0411280:CDS
AAGGACATGGATTTAGCTAA+GGG 0.609717 3_2:-53486670 None:intergenic
AAGATAAGGGAAGATTGCCA+AGG 0.672929 3_2:+53486592 Msa0411280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAATATACCTATTTATCA+AGG + chr3_2:53486555-53486574 Msa0411280:CDS 20.0%
!!! TGAATTTGTTTTGTCTTGTT+GGG + chr3_2:53486439-53486458 Msa0411280:CDS 25.0%
!! TTATTGCAAGTTTTACTAAG+TGG + chr3_2:53486519-53486538 Msa0411280:CDS 25.0%
!!! CTTTTTCTAAGGAAAGATAA+GGG + chr3_2:53486579-53486598 Msa0411280:CDS 25.0%
!!! CTGAATTTGTTTTGTCTTGT+TGG + chr3_2:53486438-53486457 Msa0411280:CDS 30.0%
AGCAAGTGTTACATTATTTG+AGG + chr3_2:53486491-53486510 Msa0411280:CDS 30.0%
! AGTTTTACTAAGTGGTGTTT+TGG + chr3_2:53486527-53486546 Msa0411280:CDS 30.0%
AGAAAAAGGCCTTGATAAAT+AGG - chr3_2:53486567-53486586 None:intergenic 30.0%
! TTTATCAAGGCCTTTTTCTA+AGG + chr3_2:53486568-53486587 Msa0411280:CDS 30.0%
CCTTATCTTTCCTTAGAAAA+AGG - chr3_2:53486581-53486600 None:intergenic 30.0%
!! CCTTTTTCTAAGGAAAGATA+AGG + chr3_2:53486578-53486597 Msa0411280:CDS 30.0%
TGCAATTACATCAACAACAT+TGG + chr3_2:53486620-53486639 Msa0411280:CDS 30.0%
TCTTGTTGGGATGTTGCTAA+AGG + chr3_2:53486452-53486471 Msa0411280:CDS 40.0%
AAGATAAGGGAAGATTGCCA+AGG + chr3_2:53486592-53486611 Msa0411280:CDS 40.0%
GATGTAATTGCAACAGACCT+TGG - chr3_2:53486612-53486631 None:intergenic 40.0%
TGTTGGGATGTTGCTAAAGG+AGG + chr3_2:53486455-53486474 Msa0411280:CDS 45.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 53486435 53486680 53486435 ID=Msa0411280;Name=Msa0411280
chr3_2 mRNA 53486435 53486680 53486435 ID=Msa0411280-mRNA-1;Parent=Msa0411280;Name=Msa0411280-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|81
chr3_2 exon 53486435 53486680 53486435 ID=Msa0411280-mRNA-1:exon:10687;Parent=Msa0411280-mRNA-1
chr3_2 CDS 53486435 53486680 53486435 ID=Msa0411280-mRNA-1:cds;Parent=Msa0411280-mRNA-1
Gene Sequence

>Msa0411280

ATGCTGAATTTGTTTTGTCTTGTTGGGATGTTGCTAAAGGAGGTTGTGTTAAGTAGAGCAAGTGTTACATTATTTGAGGCAATGTTATTGCAAGTTTTACTAAGTGGTGTTTTGGTTCTTATCAATATACCTATTTATCAAGGCCTTTTTCTAAGGAAAGATAAGGGAAGATTGCCAAGGTCTGTTGCAATTACATCAACAACATTGGCTCTAAGTGCATGTGTCCTCTATAGTGCCCTTAGCTAA

Protein sequence

>Msa0411280

MLNLFCLVGMLLKEVVLSRASVTLFEAMLLQVLLSGVLVLINIPIYQGLFLRKDKGRLPRSVAITSTTLALSACVLYSALS*